DE202013102057U1 - Device for extracting nucleic acids by means of magnetic beads - Google Patents

Abstract

A device for extracting nucleic acids by means of magnetic beads (100, 200, 300) comprises: a first reagent cassette (111, 201, 301) which has a plurality of test tubes (15) arranged next to one another, around the magnetic beads (1) and at least one of the To receive the test specimen, a separation and cleaning device for the test specimen by means of magnetic beads (112, 202, 302) to perform the test specimen, the separation method and the extraction method, wherein the separation and cleaning device for the specimen by means of magnetic beads (112, 202, 302 ), a frame (204, 304) and a magnetic control device (210), wherein the frame (204, 304) carries the first reagent cartridge (111, 201, 301), wherein the magnetic control device (210) magnetic force for the movement of the magnetic Beads (1) provides to initiate the nucleic acid extraction method and to recover the extract (214, 314), a detection vessel (113, 203, 303) to receive the extract (214, 314) and a detection device (120, 220, 320), wherein the detection device (120, 220, 320) comprises a receiving area (205, 305), a UV light source (1201) and a sensor unit (1205, 2205, 3205), the receiving area (205, 305) receiving the detection vessel (113, 203, 303), the UV light source (1201) comprising a UV light beam having a plurality of different wavelengths and then projected onto the detection vessel (113, 203, 303), wherein the sensor unit (1205, 2205, 3205) is arranged on the movement path of the light beam in order to pass through the extract (214, 314) of the detection vessel (113, 203, 303) received light beam.

Description

Technical area

The present invention relates to an extraction device, in particular to an extraction device for obtaining nucleic acids.

State of the art

Nowadays, nucleic acid analysis is an indispensable method in medical diagnostics or biomedical research. Therefore, technological development in the separation and extraction of nucleic acids is rapid and already quite mature. How to efficiently obtain nucleic acids of high quality and high purity from various organic specimens is a development goal of the field. For example, magnetic particles (hereinafter referred to as "magnetic beads") having specific biomolecules or functional groups specifically adsorb the nucleic acids from the mixture solution of the specimen, and then together with the magnetic control device (FIG. a magnet) provides the magnetic force or magnetic fields to attract and agitate the magnetic beads to facilitate the various stages of nucleic acid extraction, namely reaction, washing and extraction. This method is referred to herein as "magnetic bead nucleic acid extraction method".

The method and theory of the conventional nucleic acid extraction method using magnetic beads is performed according to the method described in 1 shown flow chart, which is briefly described below: The test specimen in which the lysis process was performed, the mixture solution of test specimen and lysis buffer 11 and the quantitative magnetic beads 1 be first in the test tube 15 loaded. In the meanwhile, the nucleic acids of the test piece are dissolved in the mixture solution, so that the magnetic beads 1 the nucleic acids of the mixture solution of test specimen and lysis buffer 11 Then, the magnetic beads together with the test specimen by a magnet 10 then the solution in the test tube 15 aspirated, then the first buffer solution 12 in the test tube 15 infiltrated, then the contaminants of the nucleic acid adsorbing magnetic beads 1 washed away, then through the magnet 10 the nucleic acid adsorbing magnetic beads 1 then the solution in the test tube 15 sucked off, then the second buffer solution 13 in the test tube 15 infiltrated, then once again the contaminants of the nucleic acid adsorbing magnetic beads 1 washed away, then by the magnet 10 the nucleic acid adsorbing magnetic beads 1 and then the solution in the test tube 15 aspirated. However, the steps of flushing away by means of the buffer solution for the nucleic acid adsorbing magnetic beads 1 adapted to the user's needs, depending on the desired cleaning level, in order to increase flexibility, whereby the steps of flushing away can be reduced to a single pass or increased to multiple passes. Finally, the elution buffer solution 14 into the test tube 15 filled in to the nucleic acid adsorbing magnetic beads 1 to elute, whereby the nucleic acid extract can be obtained, at which moment the nucleic acid extract already in the elution buffer solution 14 is solved. Finally, the magnetic beads 1 by means of the magnet 10 attracted and the solution in the test tube 15 then sucked off. This solution represents a mixed solution containing the nucleic acid extract.

Since the above-mentioned magnetic bead extraction and purification method using magnetic beads uses different magnetic beads with special magnetic field conditions which cause aggregation or dispersion of the magnetic beads, the complicated mechanical processes required, unlike the other commonly used conventional centrifugal Nucleic acid extraction "can be saved. Therefore, the extraction of nucleic acids by means of magnetic beads is ideal for automation and able to deal with a large number of specimens within a short time, thereby ensuring a high throughput in the extraction of nucleic acids. The fully automatic operation system replaces the manual processes to reduce the labor demand, so that not only efficiency improvement and economic advantages but also reduction of problems, such as e.g. human errors in guidance, cross-contamination of specimens and the high risk of specimen infection can be achieved.

By means of the automatic devices for the extraction of nucleic acids by means of magnetic beads which are common in the market, only the test body separation methods and the nucleic acid extraction methods can be carried out in order to obtain nucleic acid extracts. However, the quality of the nucleic acid extracts in this phase plays a crucial role for the subsequent nucleic acid analysis, which is why a qualitative and quantitative examination must be carried out on the nucleic acid extracts. This examination is usually done by a manual method, wherein the application of, for example, the spectrophotometer, the purity and content of the nucleic acid extracts is examined, first the quality of the nucleic acid extracts must be checked so that the nucleic acid extracts can then be used for later applications or diagnostic analysis. As this process requires manual procedures and the use of other equipment, the overall nucleic acid analysis procedure increases time and costs for staff, and also increases the risk of human error and cross-contamination.

Accordingly, the efficiency in the extraction of nucleic acids, taking into account the quality control of nucleic acid extracts and also the simplification of the nucleic acid extraction method, represents an urgent problem for the current magnetic bead nucleic acid extraction method system.

Object of the invention

With the aim of overcoming or solving the mentioned disadvantages and problems, the present invention provides a device for extracting nucleic acids by means of magnetic beads, which uses the magnetic bead automated nucleic acid extraction method system with the spectrophotometric system for qualitative and quantitative analysis of nucleic acid extracts combined.

Another object of the present invention is to provide a magnetic bead extraction apparatus which combines the magnetic bead automatic nucleic acid extraction method system with the spectrophotometric system for qualitative and quantitative analysis of nucleic acid extracts to increase comfort. to reduce equipment costs while improving the overall operational efficiency of nucleic acid extraction and quality control.

The present invention relates to a device for extracting nucleic acids by means of magnetic beads, which comprises a first reagent cassette, which in turn includes a plurality of juxtaposed test tubes to receive the magnetic beads and at least one of the test specimens, a separation and cleaning device for the specimen by means of magnetic beads for carrying out the separation method and the extraction method in the above-mentioned test piece, the magnetic bead separating and cleaning apparatus including a frame and a magnetic control device, the frame carrying the first reagent cassette, the magnetic control means having magnetic force for moving the test piece magnetic beads to initiate the nucleic acid extraction method and recover the extract, a detection vessel to receive the extract and a detection device, wherein the Det tion device includes a receiving area, a UV light source and a sensor unit, wherein the receiving area receives the detection vessel, wherein the UV light source provides a UV light beam having a plurality of different wavelengths and then projected onto the detection vessel, wherein the sensor unit arranged on the movement path of the light beam is to receive the light beam passed through the extract of the detection vessel.

The apparatus for extracting nucleic acids by means of magnetic beads and the detection vessel may be arranged adjacent to the test tubes of the first reagent cassette in order to receive the solutions of the nucleic acid extracts and at the same time carry out a direct quantitative and qualitative examination of the nucleic acid extracts.

The device for extracting nucleic acids by means of magnetic beads may further comprise a control device and an output device, wherein the control device for the control and operation of the device for extracting nucleic acids by means of magnetic beads and is responsible for the detection device and the output device for the output of a plurality of electrical Signals is responsible.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the first reagent cassette may further receive a plurality of extraction reagents or mixtures of the test specimens and the extraction reagents.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the first reagent cassette may include at least one magnetic separation vessel used with the magnetic control device, separating the magnetic beads in this separation vessel from the sample to facilitate separation of the magnetic beads guarantee.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the detection device may include a condenser lens, a light distributor unit and at least one refractor, wherein all are arranged on the movement path of the UV light beam, wherein in the light distribution unit at least one filter mirror is arranged on the movement path of the UV light beam to convert the output UV light beam into a UV light beam having a plurality of different wavelengths.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the detection device may include a signal evaluation unit which can convert the light beam received by the sensor unit into a plurality of optical signals, wherein the signal evaluation unit includes software or firmware, whereby the analysis of the optical signals by means of the software or the firmware can be performed, and then the optical signals are converted into several electrical signals and output, which represent the extinction values of the extracts.

A further object of the present invention is to provide a device for extracting nucleic acids by means of magnetic beads, which combines the automatic nucleic acid extraction method system using magnetic beads with the spectrophotometric system for the qualitative and quantitative analysis of nucleic acid extracts in order to increase comfort, to reduce equipment costs while improving the overall operational efficiency of nucleic acid extraction and quality control.

Based on the above considerations of the present invention, the present invention offers another preferred embodiment of the magnetic bead extraction device comprising a first reagent cartridge, which in turn includes a plurality of test tubes arranged side by side to surround the magnetic beads and at least one To receive the test specimen, a separation and cleaning device for the test piece by means of magnetic beads to perform the above test specimen, the separation method and the extraction method, wherein the separation and cleaning device for the specimen by means of magnetic beads includes a frame and a magnetic control device, said Frame carries the first reagent cassette, wherein the magnetic control device provides a magnetic force for the movement of the magnetic beads to initiate the nucleic acid extraction method and recovering the extract, a second reagent cassette having a plurality of juxtaposed detection vessels for receiving the extract and at least one detection device, the detection device including a receiving area, a UV light source and a sensor unit, the receiving area receiving the detection vessel of the second reagent cassette wherein the UV light source provides a UV light beam having a plurality of different wavelengths and then projecting onto the detection vessel, wherein the sensor unit is arranged on the movement path of the light beam to receive the light beam passed through the extract of the detection vessel.

The above-mentioned device for extracting nucleic acids by means of magnetic beads may further include a control device and an output device, wherein the control device is responsible for the control and operation of the device for extracting nucleic acids by means of magnetic beads and for the detection device and the output device for the output is responsible for several electrical signals.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the first reagent cassette may further receive a plurality of extraction reagents or mixtures of the test specimens and the extraction reagents.

According to the above-mentioned device for extracting nucleic acids by means of magnetic beads, the first reagent cassette may include at least one magnetic separation vessel used with the magnetic control device, separating the magnetic beads in this separation vessel from the sample to facilitate separation of the magnetic beads guarantee.

In summary, the magnetic bead extraction device of the present invention has the advantages of the above-mentioned magnetic bead automated nucleic acid extraction method system, not only for effectively separating and recovering the nucleic acid extracts from the specimen, but also for quality control and detection to perform the qualitative and quantitative analysis of nucleic acid extracts using the same device, so as to improve the ease of use, lower the equipment costs, and also improve the overall operational efficiency of nucleic acid extraction and quality control. The nucleic acid magnetic bead extraction apparatus of the present invention is very useful for high-throughput nucleic acid extraction and analysis and thus can be used as a more suitable nucleic acid analysis in biomedical research and clinical diagnostics.

Brief description of the illustrations

1 Fig. 10 is a flow chart of the operation process of the conventional nucleic acid magnetic bead extraction method;

2 Fig. 10 is a block diagram of the magnetic bead extraction apparatus of the present invention;

3 Fig. 12 is a side view of the detection state of the magnetic bead extraction apparatus of the first embodiment of the present invention;

4A Fig. 12 is a plan view of the second reagent cartridge of the magnetic bead extraction apparatus of the first embodiment of the present invention;

4B Fig. 12 is a front view of the second reagent cartridge of the magnetic bead extraction apparatus of the first embodiment of the present invention;

5 Fig. 12 is a side view of the magnetic bead extraction apparatus of the second embodiment of the present invention;

6 Fig. 12 is a side view of the detection state of the magnetic bead extraction apparatus of the second embodiment of the present invention;

7 Fig. 10 is an enlarged front view of the detection device of the magnetic bead extraction apparatus of the second embodiment of the present invention.

Detailed description of the embodiments

In order to provide those skilled in the art with a better understanding of the objects, technical characteristics and advantages of the present invention, the technical features and preferred embodiments will be described hereinafter together with the accompanying drawings, but the description of the following embodiments is not limited of the present invention. The following accompanying figures illustrate only schemes for the features of the present invention.

2 FIG. 3 provides a schematic block diagram of the device for extracting nucleic acids using magnetic beads. FIG 100 The device for the extraction of nucleic acids by means of magnetic beads 100 includes a separation and cleaning group for the test piece by means of magnetic beads 110 , a detection device 120 , a control device 130 and an output device 140 , The separation and purification method of nucleic acids in the device for extraction of nucleic acids by means of magnetic beads 100 includes a "separation method" and a "cleaning method", wherein the control device 130 the user for the adjustment, control and operation of the device for the extraction of nucleic acids by means of magnetic beads 100 and the detection device 120 serves, the output device 140 outputs the results of the qualitative and quantitative analysis of the nucleic acid extracts.

The "separation method" in the magnetic bead extraction apparatus of the present invention generally refers to biological specimens or clinical specimens prepared by physical methods, chemical methods, or lysis method for enzymes, etc. (such as mechanical polishing, etc.). High salt concentration buffer, trypsin cleavage, organic solvent separation method) lyses the specimen or sample so that the nucleic acids in the cells of the specimen or specimen are completely released in the lysis buffer. The "purification method" refers to further extraction steps for the lysis buffer containing the nucleic acids to recover the nucleic acid extracts.

The separation and cleaning group for the test specimen by means of magnetic beads 110 includes at least a first reagent cartridge 111 , A separation and cleaning device for the specimen by means of magnetic beads 112 and a detection vessel 113 which are devices and components related to the separation and purification of specimens, the device being used for the separation process and for the purification process on biological specimens or clinical specimens to recover the nucleic acid extracts, the nucleic acid being Extracts are normally in the dissolved state and further secondary separation and purification processes, drying processes or investigations can be carried out as required.

The detection device 120 the device for the extraction of nucleic acids by means of magnetic beads 100 is opposite to the corresponding respective detection vessel to be detected 113 arranged. Therefore, the same device for extraction of nucleic acids using magnetic beads 100 several detection devices 120 include the respective optical detection method of the detection vessel 113 and perform the qualitative and quantitative procedures on the nucleic acid extracts.

The detection device 120 the device for the extraction of nucleic acids by means of magnetic beads 100 The present invention includes a UV light source 1201 , a condensing lens 1202 , a light distribution unit 1203 , a filter mirror 12031 , a refractor 1204 , a sensor unit 1205 and a signal evaluation unit 1206 , where the UV light source 1201 provides a UV light beam, wherein the light distribution unit 1203 and the filter mirror 12031 in the light distribution unit 1203 responsible for the division of the UV light beam into different wavelengths, wherein the convergent lens 1202 and the refractor 1204 , which are such optical elements, are arranged on the trajectory of the light beam to pass the UV light beam through the container wall of the container receiving the nucleic acid extract and to irradiate the nucleic acid extract. Then the sensor unit starts 1205 , which is also arranged on the trajectory of the light beam, the UV light beam with a specific wavelength section, wherein the nucleic acid extract previously adsorbed the UV light beam, then reflected a specific wavelength section and then by means of software or firmware the signal evaluation unit 1206 The various optical signals are analyzed to that of the sensor unit 1205 converted light beam into optical signals, in turn, then convert the optical signals into corresponding electrical signals and through the data output to the output device 140 The user then reads the data analysis by the output device 140 can receive. The quantitative and qualitative examination of nucleic acid extracts is carried out according to the principle described above.

In connection with the UV light source 1201 is to be mentioned as further explanation that when multiple samples are to be detected, the UV light source 1201 can be regulated by their number as needed. To achieve a quick examination, can be done by a single UV light source 1201 the required for multiple samples UV light beam are provided to perform the quantitative and qualitative investigation process quickly. When the detection signal is to be amplified or the detection sensitivity is to be increased, a light source for each sample may be used to achieve the above-mentioned object.

The method of operation and mode of operation of the nucleic acid extraction system using magnetic beads have already been briefly described in previous sections of the text. The technique used is common and easy to understand for persons having general knowledge in the relevant technical fields, so the description in the following text will not be detailed. The following describes the preferred embodiments of the present invention.

First embodiment

3 , which is a side view of the device for extracting nucleic acids by means of magnetic beads 200 The first embodiment of the present invention shows the detection state. The device for extraction of nucleic acids by means of magnetic beads 200 The present embodiment includes a first reagent cartridge 201 , A separation and cleaning device for the specimen by means of magnetic beads 202 , a detection vessel 203 a frame 204 , a magnetic control device 210 and a detection device 220 ,

According to the first embodiment of the present invention, the frame becomes 204 for carrying the first reagent cartridge 201 used. In the present invention, the configuration of the frame 204 not limited. The construction of the frame 204 may be according to the number of reagents of the first reagent cassette 201 and the spatial arrangement of all components of the device for extraction of nucleic acids by means of magnetic beads 200 be configured in optimized form. The magnetic control device 210 is responsible for providing the magnetic force to attract, move and move the magnetic beads. In the present invention, the source of the magnetic force of the magnetic control device 210 not limited. For example, natural magnets, artificial magnets or electromagnets can be used. In addition, the details of the separation procedure and method of extracting nucleic acids and the related operation of the pipette device are in themselves known to a person skilled in the art, and therefore the details will not be described further here.

Furthermore, in the present invention, the magnetic beads generally refer to the particles that have the ability to be attracted and controlled by magnetic force or magnetic fields. The particle size distribution can range from the micron to the nanoscale range. The materials and the modifications of the surfaces can be chosen according to the requirements. Which magnetic beads are suitable for the present invention depends primarily on which of them To control magnetic force or magnetic fields. In addition, it will be readily apparent to one of ordinary skill in the art that when using the magnetic beads, because of their particle shapes, typically corresponding amounts of suspending fluid of the specific magnetic beads are used in accordance with the adsorption capacities and other requirements of certain magnetic beads for particular nucleic acids to access a fixed number of magnetic beads.

See again 3 In the present embodiment, the first reagent cartridge 201 a plurality of test tubes arranged side by side, wherein each of the test tubes can receive the necessary materials for the separation and extraction of nucleic acids. The materials may be organic samples, biopsies, clinical samples, reagents (eg, organic solvents, reagents, buffer solutions and enzyme solutions) or magnetic beads. In other words: the test tubes of the first reagent cassette 201 provide several separate and independent receptacles for the individual reactions of the specimens, reagents or enzymes in the separation and purification process by the device for extraction of nucleic acids by means of magnetic beads 200 at. In order to improve the effect of rinsing or separation of the magnetic beads, further, one or more test tubes of the first reagent cassette 201 in the specific form of the magnetic separation tank 2011 be educated.

The detection vessel 203 in 3 serves to hold the liquid extract and is opposite to the detection device 220 arranged. The detection device 220 has a recording area 205 for receiving the detection vessel 203 on, leaving the detection vessel 203 , the UV light source (not shown in the figures) and the sensor unit 2205 are arranged on the trajectory of the UV light beam to ensure the qualitative and quantitative analysis of the extracts.

In the device for extraction of nucleic acids by means of magnetic beads 200 In the present embodiment, a pipette 206 used for transferring or replacing the liquid contents in the test tube. The use and application of the pipette system are in and of themselves known to a person skilled in the art, for which reason the details will not be described in detail here.

According to the aforementioned nucleic acid extraction method, by the separation and purification method, the nucleic acid extract (hereinafter simply referred to as extract) can be obtained, whereby the extract is separated from the magnetic beads and in the elution buffer in the elution step of the separation and purification process in addition, the qualitative and quantitative analysis of the extract in the present invention is carried out in fluid form.

The qualitative and quantitative analysis of nucleic acid extracts is carried out by an optical method, which is why the detection vessel 203 according to the detecting need of the optical method preferably has a good UV-transmission, so that a sufficiently strong UV-light beam through the wall of the detection vessel 203 on the extract within the detection vessel 203 arrives and irradiates it. Subsequently, the UV light beam radiates again through the wall of the detection vessel 203 out, leaving the sensor unit 2205 the detection device 220 can receive a sufficiently strong transmitted UV light beam to perform the subsequent detection and analysis. Therefore, it is necessary that the detection vessel 203 preferably made of a material with good UV transmission. When choosing the material reusable highly transparent quartz glass, plastic and resin material are to be preferred. When using plastic or resin material, the light transmittance of the UV light beam must be more than 50% higher than that of the quartz material in order to be suitable for the detection device 220 to be suitable, such. Polymethyl methacrylate (PMMA), methyl methacrylate (MMA) and other plastic composite materials. In terms of disposable use, convenience, ease of manufacture, and contamination prevention, the above-mentioned plastic materials or resin materials having good transparency are the preferred materials of the present invention.

Because the reagents for nucleic acid extraction are diverse and contain some potentially highly corrosive organic solvents (such as phenol or chloroform) or have extreme pH values in the chemical reagents due to strong acids and strong alkalis, the reagents in the In addition, separation techniques and extraction procedures are also frequently heated to increase the effect of the reaction are the materials of the first reagent cassette 201 and the detection vessel 203 preferably made of a material having a high degree of chemical resistance.

In the device for extraction of nucleic acids by means of magnetic beads 200 At the same time, multiple first reagent cartridges may be used to perform separation and extraction procedures on the various specimens, with the detection vessel 203 according to the design of the first reagent cassette 201 is configured, which is why the detection vessel 203 a plurality of adjacent, tubular container may be arranged. See the second reagent cassette for this 203a in 4A and 4B , in which 4A a perspective top view, while 4B is a front view. The two figures show the second reagent cartridge 203a the device for the extraction of nucleic acids by means of magnetic beads 200 the first embodiment of the present invention. The second reagent cassette 203a includes a test tube holder 2032 and a test tube holder 2032 carrying rack 2031 , where the frame 2031 opposite the test tube holder 2032 is arranged. For the second reagent cartridge 203a Depending on the manufacturing requirements, one-piece disposable reagent cartridges may be used which consist of a test tube holder 2032 and a rack 2031 may or may be in accordance with the overall operation requirement and along with the assembly and operation of the components of the device for extracting nucleic acids by means of magnetic beads 200 Two-part molds for the test tube holder 2032 and the frame 2031 which can be assembled and disassembled to facilitate disassembly and assembly in the manufacturing and application process. If for the second reagent cartridge 203a the two-part design is used is the material selection of the test tube holder 2032 not limited, but care should be taken that the selection is in accordance with the various needs of the user (such as high UV transmission, disposable use, lightness, ease of manufacture, and stain resistance).

The by the separation and extraction method of the device for extraction of nucleic acids by means of magnetic beads 200 Extracted extract is through the automatic pipetting device in the detection vessel 203 transfilled and the quantitative and qualitative examination procedure subsequently together with the detection device 220 carried out.

Please refer 3 : The detection device 220 the device for the extraction of nucleic acids by means of magnetic beads 200 has a downwardly indented recording area 205 on to the in the detection vessel 203 to accommodate accommodated extract, so that the detection vessel 203 and the extract on the trajectory of the UV light beam (hereinafter simply referred to as trajectory) are arranged, wherein the detection device 220 is arranged correspondingly in the end portion of the movement path, so that the UV light beam is divided into specific wavelengths by the light distribution unit (not shown in the figures), which is also arranged on the movement path and includes a filter mirror. Depending on the substances to be detected (eg DNA, RNA or proteins) and the corresponding requirements for the different wavelengths of the UV light beam, the user can use several filter mirrors to obtain a UV light beam with different wavelengths, which together with different refractors , Lenses and optical elements (light-collecting lens, astigmatic lens), which are also arranged on the movement path, are used, wherein the UV light beam with certain wavelengths along the movement path is emitted and the detection vessel 203 crosses. After the UV light beam has been irradiated onto the extract, the UV light beam can turn the wall of the detection vessel 203 traverse and shine out, leaving the sensor unit 2205 can receive the optical signals resulting from the irradiation of the UV light beam to enable the subsequent optical signal analysis.

According to the aforementioned method, the detection device 220 additionally a signal evaluation unit (not shown in the figures), software or firmware to analyze the optical signals. According to the above-mentioned method, the extract is irradiated by the ultraviolet ray on the above-mentioned trajectory, then it is irradiated by the sensor unit 2205 detected by the sensor unit 2205 received light beam through the signal evaluation unit of the detection device 220 is converted into various optical signals, wherein the aforesaid software or firmware then executes the predetermined operational commands to analyze the optical signals, subsequently convert them to electrical signals and finally output by the output device. The output signals appear in the form of numerical data reflecting the extinction values of the extract, the extinction values being the numerical values of the original signals, the numerical values after calculation, the numerical values reflected by different wavelengths of the UV light beam, or the relative relationship between can represent the aforementioned different values. All these different values can be used for the qualitative or quantitative analysis of the concentration and quality of extracts.

Second embodiment

Please refer 5 and 6 , Side view of a device for extraction of nucleic acids by means of magnetic beads 300 according to the second embodiment of the present invention. The two figures show the respective situations with a Detection state and with a non-detection state. The device for extraction of nucleic acids by means of magnetic beads 300 includes a first reagent cartridge 301 , A separation and cleaning device for the specimen by means of magnetic beads 302 , a detection vessel 303 a frame 304 and a detection device 220 , The separation and cleaning device for the test specimen by means of magnetic beads 302 The nucleic acid separation and purification method, the pipetting device, the pipetting operation mode, and the optical detection method are substantially the same as those of the first embodiment in the second embodiment of the present invention, and will not be discussed in detail here. To provide a simple description for those skilled in the art, the related elements that are identical to the elements in the first embodiment are not shown in the figures.

It should be noted that the differences between the device for extraction of nucleic acids by means of magnetic beads 300 the second embodiment and the device for extraction of nucleic acids by means of magnetic beads 200 The first embodiment of the present invention resides in that the detection vessel 303 that the extract 314 in the first reagent cartridge 301 is arranged, bringing the detection vessel 303 in proximity to the test tubes of the first reagent cassette 301 located. The detection vessel 303 also has a high UV light transmission, which is why the UV light beam easily through the wall of the detection vessel 303 can radiate through, then the extract 314 irradiated and again through the detection vessel 303 emits to the sensor unit 3205 the detection device 320 with a sufficiently strong UV light beam and perform the subsequent detection and analysis.

Due to the arrangement of the detection vessel 303 in such a way, in the first reagent cartridge 301 in the second embodiment of the present invention, no further additional separation vessel is used, ie the first reagent cassette 301 of the present embodiment can be used directly for the quantitative and qualitative assay of extracts, thereby facilitating the operation and additional related equipment such as the detection vessel, the reagent cartridge, the detection element and drive elements for the detection vessel and for the reagent cartridge or to save pipetting elements. Therefore, the present embodiment can contribute to the reduction of the volume and the saving in the manufacturing cost of the equipment. Such a construction also allows the detection vessel 303 directly for the detection device 320 can be used, ie a simplification of the pipetting steps achieved and thus the time for the quantitative and qualitative examination method of extracts is shortened.

7 shows an enlarged front view of the detection device 320 the device for the extraction of nucleic acids by means of magnetic beads 300 the second embodiment of the present invention. Because the detection vessel 303 into the first reagent cartridge 301 is integrated and can be used directly for the detection of the extracts, the design of the detection device 320 the device for the extraction of nucleic acids by means of magnetic beads 300 adapted accordingly, so that at this side a receiving area 305 is provided to the in the detection vessel 303 to accommodate accommodated extract, so that the detection vessel 303 and the extract on the trajectory of the UV light beam (hereinafter simply referred to as trajectory) are arranged, wherein the detection device 320 is arranged correspondingly in the end portion of the movement path, so that the UV light beam is divided into specific wavelengths by the light distribution unit (not shown in the figures), which is also arranged on the movement path and includes a filter mirror. Depending on the substances to be detected (eg DNA, RNA or proteins) and the corresponding requirements for the different wavelengths of the UV light beam, the user can use several filter mirrors to obtain a UV light beam with different wavelengths, which together with different refractors , Lenses and optical elements (light-collecting lens, astigmatic lens), which are also arranged on the movement path, are used, wherein the UV light beam with certain wavelengths along the movement path is emitted and the detection vessel 303 crosses. After the UV light beam has been irradiated onto the extract, the UV light beam can turn the wall of the detection vessel 303 traverse and shine out, leaving the sensor unit 3205 can receive the optical signals resulting from the irradiation of the UV light beam to enable the subsequent optical signal analysis. The analysis of the signals is carried out in substantially the same way as described in the first embodiment.

Please refer 7 and at the same time 5 and 6 to understand the detection method of the present embodiment. When the quantitative and qualitative assay procedure is performed on the extracts, the detection vessel is 303 in the lateral receiving area 305 arranged as in 6 shown. The detection vessel 303 and the detection device 320 Both are arranged on the trajectory. The UV light beam for the detection moves along the trajectory and passes through the UV irradiation opening 3207 . 3208 the detection device 320 in the recording area 305 and then radiates through the area of UV radiation 3031 of the detection vessel 303 therethrough. The UV light beam then further irradiates the extract and is removed from the detection vessel 303 blasted to the outside. The transmitted light beam is from the sensor unit 3205 the detection device 320 intercepted and further processed, and finally, as described above, the transformation of the signals and the output of the data are performed by means of the related elements.

The material selection of the detection vessel 303 the first reagent cartridge 301 In the present embodiment, in principle, the same as in the first embodiment, whereby highly transparent plastics are to be preferred in the implementation.

According to the above-mentioned thoughts, aims, demonstrations and embodiments of the present invention, the magnetic bead extraction device integrates the current automatic nucleic acid extraction method system using magnetic beads and the subsequent optical system of the quantitative and qualitative assay methods of the extract such that the Device for extracting nucleic acids by means of magnetic beads of the present invention has the functions of both.

The device for extracting nucleic acids by means of magnetic beads of the present invention also has the advantages of the magnetic bead automated nucleic acid extraction method system, wherein the quantitative and qualitative assay methods of the extract and the quality control can be performed on the same device, so that the Increases efficiency and comfort in the separation and purification of nucleic acids and quality control is improved, thereby simplifying the device setup and operations and human errors or omissions that are associated with the complicated work in the conventional art, and thus reduce to increase the suitability of the magnetic bead extraction device of the present invention for biomedical research and clinical diagnosis of nucleic acid analysis.

The present invention thus relates to a device for extracting nucleic acids by means of magnetic beads, which comprises a first reagent cassette, which in turn contains a plurality of test tubes arranged side by side to receive the magnetic beads and the test piece, a separation and cleaning device for the test piece by means of magnetic beads, to recover the extract by the separation method and the extraction method, a detection vessel to receive the extract and a detection device, the detection device including a receiving area, a UV light source and a sensor unit, the receiving area receiving the detection vessel, the UV light source provides a UV light beam having a plurality of different wavelengths and then projected onto the detection vessel, wherein the sensor unit is arranged on the movement path of the light beam to that through the extract of the detection vessel The optical signals are converted into electrical signals, whereby the extinction values are displayed and thus a quantitative and qualitative examination of the extract is ensured.

LIST OF REFERENCE NUMBERS

1

magnetic beads

10

magnet

11

Mixing solution of test specimen and lysis buffer

12

first buffer solution

13

second buffer solution

14

elution buffer

15

Test tube

100, 200, 300

Device for extracting nucleic acids by means of magnetic beads

110

Separation and cleaning group for the test specimen by means of magnetic beads

111, 201, 301

first reagent cartridge

2011, 3011

magnetic separation tank

112, 202, 302

Separation and cleaning device for the test specimen by means of magnetic beads

113, 203, 303

detection vessel

120, 220, 320

detection device

1201

UV-light source

1202

converging lens

1203

Light distribution unit

12031

filter mirror

1204

refractor

1205-2205-3205

sensor unit

1206

signal evaluation

130

control device

140

output device

203a

second reagent cartridge

2031

frame

2032

test tube holder

204-304

frame

210

magnetic control device

214-314

extract

205-305

reception area

206

pipette

3031

Range of UV radiation

3207-3208

UV irradiation port

Claims (14)

A device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) includes: a first reagent cartridge ( 111 . 201 . 301 ), which contains several test tubes ( 15 ) to the magnetic beads ( 1 ) and at least one of the test specimens, a separation and cleaning device for the specimen by means of magnetic beads ( 112 . 202 . 302 ) in order to carry out the separation process and the extraction process in the test specimen, the test specimen separation and cleaning device being prepared by means of magnetic beads ( 112 . 202 . 302 ) a frame ( 204 . 304 ) and a magnetic control device ( 210 ), whereby the frame ( 204 . 304 ) the first reagent cassette ( 111 . 201 . 301 ), wherein the magnetic control device ( 210 ) Magnetic force for the movement of the magnetic beads ( 1 ) to initiate the nucleic acid extraction procedure and extract ( 214 . 314 ), a detection vessel ( 113 . 203 . 303 ) to the extract ( 214 . 314 ) and a detection device ( 120 . 220 . 320 ), wherein the detection device ( 120 . 220 . 320 ) a receiving area ( 205 . 305 ), a UV light source ( 1201 ) and a sensor unit ( 1205 . 2205 . 3205 ), the receiving area ( 205 . 305 ) the detection vessel ( 113 . 203 . 303 ), wherein the UV light source ( 1201 ) delivers a UV light beam with several different wavelengths and then to the detection vessel ( 113 . 203 . 303 ), whereby the sensor unit ( 1205 . 2205 . 3205 ) is arranged on the trajectory of the light beam to pass through the extract ( 214 . 314 ) of the detection vessel ( 113 . 203 . 303 ) received light beam. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1, characterized in that the detection vessel ( 113 . 203 . 303 ) adjacent to the test tubes ( 15 ) of the first reagent cassette ( 111 . 201 . 301 ) is arranged. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1 or 2, characterized in that a control device ( 130 ) and an output device ( 140 ) is provided, wherein the control device ( 130 ) for the control and the operation of the device for the extraction of nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) and for the detection device ( 120 . 220 . 320 ) and the output device ( 140 ) is responsible for the output of several electrical signals. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1 or 2, characterized in that the first reagent cassette ( 111 . 201 . 301 ) further receives a plurality of extraction reagents. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1 or 2, characterized in that the first reagent cassette ( 111 . 201 . 301 ) at least one magnetic separation vessel ( 2011 . 3011 ), wherein the magnetic separation container ( 2011 . 3011 ) together with the magnetic control device ( 210 ), the magnetic beads ( 1 ) in the magnetic separation vessel ( 2011 . 3011 ) are separated from the specimen. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1 or 2, characterized in that the detection device ( 120 . 220 . 320 ) furthermore a collecting lens ( 1202 ), a light distribution unit ( 1203 ) and at least one refractor ( 1204 ), all being located on the trajectory of the light beam. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 6, characterized in that the light distributor unit ( 1203 ) at least one filter mirror ( 12031 ) to convert the output UV light beam into a UV light beam having a plurality of different wavelengths. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 1 or 2, characterized in that the detection device ( 120 . 220 . 320 ) a signal evaluation unit ( 1206 ) by the sensor unit ( 1205 . 2205 . 3205 ) converted light beam into a plurality of optical signals. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 8, characterized in that the signal evaluation unit ( 1206 ) further includes software, whereby the analysis of the optical signals can be performed by means of the software, wherein subsequently the optical signals are converted into several electrical signals and output. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 9, characterized in that the output electrical signals are the extinction values of the extract ( 214 . 314 ). A device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ), includes: a first reagent cartridge ( 111 . 201 . 301 ), which contains several test tubes ( 15 ) to the magnetic beads ( 1 ) and at least one of the test specimens, a separation and cleaning device for the specimen by means of magnetic beads ( 112 . 202 . 302 ) in order to carry out the separation process and the extraction process in the test specimen, the test specimen separation and purification device being prepared by means of magnetic beads ( 112 . 202 . 302 ) a frame ( 204 . 304 ) and a magnetic control device ( 210 ), whereby the frame ( 204 . 304 ) the first reagent cassette ( 111 . 201 . 301 ), wherein the magnetic control device ( 210 ) Magnetic force for the movement of the magnetic beads ( 1 ) to initiate the nucleic acid extraction procedure and extract ( 214 . 314 ), a second reagent cassette ( 203a ), which has several detection vessels ( 113 . 203 . 303 ) to the extract ( 214 . 314 ) and at least one detection device ( 120 . 220 . 320 ), wherein the detection device ( 120 . 220 . 320 ) a receiving area ( 205 . 305 ), a UV light source ( 1201 ) and a sensor unit ( 1205 . 2205 . 3205 ), the receiving area ( 205 . 305 ) the detection vessel ( 113 . 203 . 303 ) of the second reagent cassette ( 203a ), wherein the UV light source ( 1201 ) supplies a UV light beam with different wavelengths and then onto the detection vessel ( 113 . 203 . 303 ), whereby the sensor unit ( 1205 . 2205 . 3205 ) is arranged on the trajectory of the light beam to pass through the extract ( 214 . 314 ) of the detection vessel ( 113 . 203 . 303 ) received light beam. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 11, characterized in that a control device ( 130 ) and an output device ( 140 ) are provided, wherein the control device ( 130 ) for the control and the operation of the device for the extraction of nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) and for the detection device ( 120 . 220 . 320 ), the output device ( 140 ) is responsible for the output of several electrical signals. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 11, characterized in that the first reagent cassette ( 111 . 201 . 301 ) further receives a plurality of extraction reagents. Device for extracting nucleic acids by means of magnetic beads ( 100 . 200 . 300 ) according to claim 11, characterized in that the first reagent cassette ( 111 . 201 . 301 ) at least one magnetic separation vessel ( 2011 . 3011 ), wherein the magnetic separation container ( 2011 . 3011 ) together with the magnetic control device ( 210 ), the magnetic beads ( 1 ) in the magnetic separation vessel ( 2011 . 3011 ) are separated from the specimen to the magnetic beads ( 1 ) to separate.

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