CN116410842A - Full-automatic nucleic acid methylation treatment equipment and treatment mechanism - Google Patents
Full-automatic nucleic acid methylation treatment equipment and treatment mechanism Download PDFInfo
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- CN116410842A CN116410842A CN202111675068.6A CN202111675068A CN116410842A CN 116410842 A CN116410842 A CN 116410842A CN 202111675068 A CN202111675068 A CN 202111675068A CN 116410842 A CN116410842 A CN 116410842A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
Abstract
The full-automatic nucleic acid methylation treatment equipment comprises a control mechanism, a sample adding mechanism and a treatment mechanism, wherein the control mechanism respectively controls the sample adding mechanism and the treatment mechanism, the treatment mechanism comprises a plurality of groups of parallel porous deep pore plates, magnetic rod rack assemblies, driving assemblies and heating assemblies, each magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves which are arranged in an array manner, and each magnetic rod is positioned above each magnetic rod sleeve and can downwards move to extend into each magnetic rod sleeve; the magnetic rod rack assembly is positioned above the porous deep pore plate, the magnetic rod rack assembly or the magnetic rod sleeve is driven by the driving assembly to move above the porous deep pore plate, and the magnetic rod rack assembly or the magnetic rod sleeve can move downwards to extend into the porous deep pore plate; the heating component is positioned below the porous deep pore plate and is used for heating liquid in the porous deep pore plate. The application also provides a processing mechanism and full-automatic nucleic acid methylation analysis equipment. In the apparatus of the present application, a high degree of automation of the methylation extraction of nucleic acids can be achieved.
Description
Technical Field
The application relates to the technical field of molecular diagnosis, in particular to full-automatic nucleic acid methylation treatment equipment and a treatment mechanism.
Background
DNA methylation is an important study of Epigenetics (Epigenetics). Changes in DNA methylation status, which include a decrease in overall methylation levels of the genome and an abnormally elevated local methylation levels of CpG islands, lead to genomic instability and thus to the induction of cellular canceration, are an important factor in the initiation of tumorigenesis.
Variations occur more frequently at the epigenetic level than at the genetic mutation, so that the use of epigenetic variations to diagnose a tumor has higher sensitivity and better specificity than detection at the genetic mutation level. Since DNA methylation is an early event in tumorigenesis, early diagnosis of tumors by detecting the methylation level of genes has significant clinical application value.
The circulating tumor DNA (ctDNA, circulating TumorDNA) is a DNA fragment from the tumor genome carried in the human blood system. The main sources are necrotic tumor cells, apoptotic tumor cells, circulating tumor cells, exosomes secreted by tumor cells. These tumor DNA often contain methylation characteristics specific for the tumor genome.
At present, manual methods are mostly adopted for purifying and sulfite treating free DNA in plasma, and even a plurality of kits can be possibly used for combining the purposes. And the manual time is long, the steps are numerous and complex, the operation pressure of more than 32 samples is greatly increased, the dead time of the front and rear samples is different due to the numerous intermediate steps, and the repeatability of the detection of the samples is not high. One of the difficulties in developing automated equipment is the handling of large volumes of sample, the volume of intermediate processing mixtures can reach 10mL, and no corresponding consumables are matched with automated equipment.
Disclosure of Invention
In view of this, the present application provides a fully automatic nucleic acid methylation processing apparatus, and the whole process from the entry of a plasma sample into the apparatus to the completion of obtaining the target DNA extraction is completed completely by the apparatus, without manual intervention, and can realize high automation of nucleic acid methylation extraction.
The technical scheme of the application is as follows:
the application provides full-automatic nucleic acid methylation treatment equipment which comprises a control mechanism, a sample adding mechanism and a treatment mechanism, wherein the control mechanism respectively controls the sample adding mechanism and the treatment mechanism,
the processing mechanism comprises a plurality of groups of parallel porous deep pore plates, a magnetic rod rack assembly, a driving assembly and a heating assembly,
The magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves which are arranged in an array manner, wherein the magnetic rods are positioned above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves;
the magnetic rod rack assembly is positioned above the porous deep pore plate, the magnetic rod rack assembly or the magnetic rod sleeve is driven by the driving assembly to move above the porous deep pore plate, and the magnetic rod rack assembly or the magnetic rod sleeve can move downwards to extend into the porous deep pore plate;
the heating component is positioned below the porous deep pore plate and is used for heating liquid in the porous deep pore plate.
Further, the driving assembly drives the magnetic rod frame assembly or the magnetic rod sleeve to extend into the porous deep hole plate and make circular movement or linear reciprocating movement for uniformly oscillating and mixing liquid in the porous deep hole plate.
Further, the processing mechanism further comprises a first bracket and a second bracket, wherein the second bracket is positioned right below the first bracket;
the processing mechanism further comprises a fourth bracket, the heating component is movably arranged on the fourth bracket, and the heating component is fixedly connected with the porous deep pore plate;
The first bracket, the second bracket and the fourth bracket are mutually parallel.
Further, m rows of magnetic bars are arranged on the first support, the distance between the m rows of magnetic bars is adjustable, each row of magnetic bars is provided with n magnetic bars, and the distance between the n magnetic bars is adjustable;
m rows of magnetic rod sleeves are arranged on the second bracket, the distance between the m rows of magnetic rod sleeves is adjustable, each row is provided with n magnetic rod sleeves, the distance between the n magnetic rod sleeves is adjustable,
wherein m is greater than or equal to 2, and n is greater than or equal to 2.
Further, the driving assembly also comprises a first driving assembly, a second driving assembly, a third driving assembly and a fourth driving assembly,
the first driving component is used for driving the first bracket to do lifting motion above the porous deep hole plate;
the second driving component is used for driving the second bracket to do lifting motion above the porous deep hole plate;
the third driving assembly is used for driving the first bracket and the second bracket to do reciprocating motion relative to the fourth bracket along a first direction above the porous deep hole plate;
the fourth driving component is used for driving the heating component and the porous deep pore plate to make reciprocating motion on the fourth bracket along a second direction;
The first direction is perpendicular to the second direction.
Further, the first driving assembly and the third driving assembly run simultaneously to drive the first bracket to do circular motion, so that the magnetic rod is driven to do circular motion;
the second driving assembly and the third driving assembly run simultaneously to drive the second bracket to do circular motion, so that the magnetic rod sleeve is driven to do circular motion.
Further, the first driving assembly comprises a first motor, a first sliding rod and a first sliding block, and the first motor drives the first sliding block to move on the first sliding rod;
the first sliding block is fixedly connected with the first bracket.
Further, the second driving assembly comprises a second motor, a second sliding rod and a second sliding block, and the second motor drives the second sliding block to move on the second sliding rod;
the second sliding block is fixedly connected with the second bracket.
Further, the processing mechanism further comprises a third bracket, the third bracket is perpendicular to the first bracket and the second bracket, the first sliding rod and the second sliding rod are arranged in parallel, and the first sliding rod and the second sliding rod are fixedly arranged on the third bracket;
The first sliding rod and the second sliding rod are perpendicular to the first bracket and the second bracket.
Further, the third driving assembly comprises a third motor, a third sliding rod and a third sliding block, and the third motor drives the third sliding block to move on the third sliding rod;
the third sliding block is fixedly connected with the third bracket.
Further, the fourth driving assembly comprises a fourth motor, a fourth sliding rod and a fourth sliding block, and the fourth motor drives the fourth sliding block to move on the fourth sliding rod;
the fourth sliding rod and the third sliding rod are fixedly arranged on the same side face of the fourth bracket;
the fourth sliding block is fixedly arranged below the heating component.
Further, the porous deep hole plate is provided with a first hole site, a second hole site and an N-th hole site which are sequentially arranged, wherein N is more than or equal to 3, and the first hole site, the second hole site and the N-th hole site are provided with a plurality of deep holes which are arranged at equal intervals;
the first hole site is used for primarily treating the liquid to be treated so as to obtain a sample to be treated;
the other hole sites between the first hole site and the N-th hole site are used for washing the sample to be treated for multiple times and converting sulfite;
And the N hole site is used for eluting the sample to be treated, so as to obtain a detection sample.
Further, the capacity of the medium deep hole in the first hole site is larger than that of the medium deep holes in other hole sites.
Further, when the N is 7, the porous deep hole plate has a first hole site, a second hole site, a third hole site, a fourth hole site, a fifth hole site, a sixth hole site, and a seventh hole site arranged in sequence;
further preferably, each hole site has 8 deep holes.
Further, the heating assembly comprises at least two mutually independent heating units;
each heating unit is provided with a plurality of heating holes;
each heating unit is used for heating the liquid in the corresponding hole site in the porous deep hole plate.
Further, the liquid to be treated is one of blood plasma, urine or epidermal tissue,
the sample to be treated is DNA containing impurities, and the detection sample is free DNA.
Further, the device also comprises a sample area, wherein the sample area comprises a liquid storage area to be treated, a reagent storage area and a liquid sample identification device to be treated, and the liquid to be treated in the liquid storage area to be treated is identified and distinguished through the liquid sample identification device to be treated.
Further, the sampling mechanism further comprises a mechanical arm and a liquid-transferring gun, wherein the mechanical arm is connected with the control mechanism and is used for controlling the liquid-transferring gun to add reagents or liquid to be treated into the porous deep hole plate.
Further, the apparatus also includes a waste storage mechanism for storing waste items after the test.
Further, the device also comprises a display mechanism, wherein the display mechanism is connected with the control mechanism and is used for giving instructions to the control mechanism.
Further, the device further comprises moving means for moving the device.
Further, the device also includes a multifunctional housing assembly including an ultraviolet disinfection unit, a lighting unit, and an operation alert unit.
The application provides a processing mechanism which comprises a plurality of groups of parallel porous deep hole plates, a magnetic rod rack assembly, a driving assembly and a heating assembly,
the magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves which are arranged in an array manner, wherein the magnetic rods are positioned above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves;
the magnetic rod rack assembly is positioned above the porous deep pore plate, the magnetic rod rack assembly or the magnetic rod sleeve is driven by the driving assembly to move above the porous deep pore plate, and the magnetic rod rack assembly or the magnetic rod sleeve can move downwards to extend into the porous deep pore plate;
The heating component is positioned below the porous deep pore plate and is used for heating liquid in the porous deep pore plate.
Further, the driving assembly drives the magnetic rod frame assembly or the magnetic rod sleeve to extend into the porous deep pore plate and make circular movement, so that liquid in the porous deep pore plate is uniformly mixed by vibration.
Further, the processing mechanism further comprises a first bracket and a second bracket, wherein the second bracket is positioned right below the first bracket;
the processing mechanism further comprises a fourth bracket, the heating component is movably arranged on the fourth bracket, and the heating component is fixedly connected with the porous deep pore plate;
the first bracket, the second bracket and the fourth bracket are mutually parallel.
Further, m rows of magnetic bars are arranged on the first support, the distance between the m rows of magnetic bars is adjustable, each row of magnetic bars is provided with n magnetic bars, and the distance between the n magnetic bars is adjustable;
m rows of magnetic rod sleeves are arranged on the second bracket, the distance between the m rows of magnetic rod sleeves is adjustable, each row is provided with n magnetic rod sleeves, the distance between the n magnetic rod sleeves is adjustable,
wherein m is greater than or equal to 2, and n is greater than or equal to 2.
Further, the driving assembly also comprises a first driving assembly, a second driving assembly, a third driving assembly and a fourth driving assembly,
the first driving component is used for driving the first bracket to do lifting motion above the porous deep hole plate;
the second driving component is used for driving the second bracket to do lifting motion above the porous deep hole plate;
the third driving assembly is used for driving the first bracket and the second bracket to do reciprocating motion relative to the fourth bracket along a first direction above the porous deep hole plate;
the fourth driving component is used for driving the heating component and the porous deep pore plate to make reciprocating motion on the fourth bracket along a second direction;
the first direction is perpendicular to the second direction.
Further, the first driving assembly and the third driving assembly run simultaneously to drive the first bracket to do circular motion, so that the magnetic rod is driven to do circular motion;
the second driving assembly and the third driving assembly run simultaneously to drive the second bracket to do circular motion, so that the magnetic rod sleeve is driven to do circular motion.
Further, the first driving assembly comprises a first motor, a first sliding rod and a first sliding block, and the first motor drives the first sliding block to move on the first sliding rod;
The first sliding block is fixedly connected with the first bracket.
Further, the second driving assembly comprises a second motor, a second sliding rod and a second sliding block, and the second motor drives the second sliding block to move on the second sliding rod;
the second sliding block is fixedly connected with the second bracket.
Further, the processing mechanism further comprises a third bracket, the third bracket is perpendicular to the first bracket and the second bracket, the first sliding rod and the second sliding rod are arranged in parallel, and the first sliding rod and the second sliding rod are fixedly arranged on the third bracket;
the first sliding rod and the second sliding rod are perpendicular to the first bracket and the second bracket.
Further, the third driving assembly comprises a third motor, a third sliding rod and a third sliding block, and the third motor drives the third sliding block to move on the third sliding rod;
the third sliding block is fixedly connected with the third bracket.
Further, the fourth driving assembly comprises a fourth motor, a fourth sliding rod and a fourth sliding block, and the fourth motor drives the fourth sliding block to move on the fourth sliding rod;
the fourth sliding rod and the third sliding rod are fixedly arranged on the same side face of the fourth bracket;
The fourth sliding block is fixedly arranged below the heating component.
Further, the porous deep hole plate is provided with a first hole site, a second hole site and an N-th hole site which are sequentially arranged, wherein N is more than or equal to 3, and the first hole site, the second hole site and the N-th hole site are provided with a plurality of deep holes which are arranged at equal intervals;
the first hole site is used for primarily treating the liquid to be treated so as to obtain a sample to be treated;
the other hole sites between the first hole site and the N-th hole site are used for washing the sample to be treated for multiple times and converting sulfite;
and the N hole site is used for eluting the sample to be treated, so as to obtain a detection sample.
Further, the capacity of the medium deep hole in the first hole site is larger than that of the medium deep holes in other hole sites.
Further, when the N is 7, the porous deep hole plate has a first hole site, a second hole site, a third hole site, a fourth hole site, a fifth hole site, a sixth hole site, and a seventh hole site arranged in sequence;
further preferably, each hole site has 8 deep holes.
Further, the heating assembly comprises at least two mutually independent heating units;
each heating unit is provided with a plurality of heating holes;
Each heating unit is used for heating the liquid in the corresponding hole site in the porous deep hole plate.
The application also provides full-automatic nucleic acid methylation analysis equipment, which comprises the full-automatic nucleic acid methylation processing equipment.
Further, one of a QPCR detection platform, an NGS detection platform or a FISH detection platform is also included.
According to the full-automatic nucleic acid methylation treatment equipment, the sample adding mechanism transfers reagents such as blood plasma, lysate and the like into the porous deep pore plate, and the blood plasma is subjected to steps such as cracking, washing, sulfite conversion and the like in the porous deep pore plate to obtain target DNA. In the device, the whole process from the entering of the plasma sample into the device to the completion of the extraction of the target DNA is completely completed by the device, and the high automation of the methylation extraction of the nucleic acid can be realized without manual intervention. Moreover, the plasma volume of the single sample processed by the device exceeds 3.5ml at one time, which is far more than the processing volume of the nucleic acid processing device in the prior art, so that the single plasma samples from a plurality of different sources can be processed at high flux, and the high flux detection can be realized.
Drawings
The drawings are included to provide a better understanding of the present application and are not to be construed as unduly limiting the present application. Wherein:
FIG. 1 is a schematic structural view of a fully automated nucleic acid methylation processing apparatus according to the present application.
FIG. 2 is a schematic structural view of a fully automated nucleic acid methylation processing apparatus of the present application.
Fig. 3 is a schematic structural view of a processing mechanism according to the present application.
Fig. 4 is a schematic structural view of a processing mechanism according to the present application.
Description of the reference numerals
1-display mechanism, 2-control mechanism, 3-sample application mechanism, 4-processing mechanism, 41-porous deep well plate, 421-magnetic bar, 422-magnetic bar sleeve, 423-first bracket, 424-second bracket, 425-first slider, 426-second slider, 43-third bracket, 44-fourth bracket, 45-first motor, 46-second motor, 47-third motor, 48-fourth motor, 49-fourth slider, 5-sample carrier seat, 6-waste gun head groove, 7-sample carrier, 8-reagent groove carrier, 9-eight-connecting tube carrier, 10-refrigeration module, 11-drawer, 12-bar code scanner, 13-lifting bar.
Detailed Description
Exemplary embodiments of the present application are described below in conjunction with the accompanying drawings, which include various details of the embodiments of the present application to facilitate understanding, and should be considered as merely exemplary. Accordingly, one of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the present application. Also, descriptions of well-known functions and constructions are omitted in the following description for clarity and conciseness.
As shown in fig. 1-2, the application discloses a full-automatic nucleic acid methylation treatment device, which comprises a control mechanism 2, a sampling mechanism 3 and a treatment mechanism 4, wherein the control mechanism 2 controls the sampling mechanism 3 and the treatment mechanism 4 respectively,
the treatment mechanism 4 comprises a plurality of groups of porous deep hole plates 41, a magnetic rod rack assembly, a driving assembly and a heating assembly (not shown) which are arranged side by side,
the magnetic rod rack assembly comprises a plurality of magnetic rods 421 and magnetic rod sleeves 422 which are arranged in an array mode, wherein the magnetic rods 421 are located above the magnetic rod sleeves 422 and can move downwards to extend into the magnetic rod sleeves 422.
The magnetic rod rack assembly is positioned above the porous deep hole plate 41, and the magnetic rod rack assembly or the magnetic rod sleeve 422 can move above the porous deep hole plate 41 and can move downwards so that the magnetic rod rack assembly or the magnetic rod sleeve 422 extends into the porous deep hole plate 41;
the driving assembly drives the magnetic rod frame assembly or the magnetic rod sleeve 422 to extend into the porous deep hole plate 41 and make circular movement or linear reciprocating movement for uniformly oscillating and mixing the liquid in the porous deep hole plate 41;
specifically, in the first hole site, the magnetic rod sleeve 422 makes a circular movement in the porous deep hole plate 41. The bar magnet sleeve 422 reciprocates linearly (i.e., reciprocates along the y-axis) within the porous deep well plate 41 at other well locations than the first well location.
The heating component is positioned below the porous deep pore plate 41 and is used for heating the liquid in the porous deep pore plate 41;
in the present application, the magnetic rod sleeve 422 may be formed of a material that is not magnetic, such as a plastic tube that is commonly used.
In this application, the sample adding mechanism 3 transfers reagents such as plasma and lysate to the porous deep well plate 41, and the plasma is subjected to steps such as lysis, washing, sulfite conversion, etc. in the porous deep well plate 41 to obtain the target DNA. In the device, the whole process from the entering of the plasma sample into the device to the completion of the extraction of the target DNA is completely completed by the device, and the high automation of the methylation extraction of the nucleic acid can be realized without manual intervention.
In this application, the number of the processing mechanisms 4 may be plural, and when the processing sample size is large, the plural processing mechanisms 4 operate simultaneously, so that plural samples can be processed rapidly, and the efficiency is high.
In this application, the handling mechanism further comprises a first support 423 and a second support 424, the second support 424 being located directly below the first support 423;
the treatment mechanism 4 further comprises a fourth bracket 44, the heating component is movably arranged on the fourth bracket 44, and the heating component is fixedly connected with the porous deep hole plate 41;
The first bracket 423, the second bracket 424, and the fourth bracket 44 are parallel to each other.
Specifically, the first support 423 is provided with m rows of magnetic bars 421, the intervals between the m rows of magnetic bars 421 are adjustable, each row has n magnetic bars 421, the intervals between the n magnetic bars 421 are adjustable, wherein m is greater than or equal to 2, and n is greater than or equal to 2; m can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., n can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., and the number of m and n can be designed according to practical needs.
When the hole positions between the porous deep hole plates 41 are equidistant, the m rows of the magnetic bars 421 are equidistant, and when the hole positions between the porous deep hole plates 41 are non-equidistant, the m rows of the magnetic bars 421 are correspondingly non-equidistant.
The adjustable spacing between adjacent magnetic bars 421 can be achieved by telescoping rods.
M rows of magnetic rod sleeves 422 are arranged on the second support 424, the intervals among the m rows of magnetic rod sleeves 422 are adjustable, each row is provided with n magnetic rod sleeves 422, the intervals among the n magnetic rod sleeves 422 are adjustable, and m is more than or equal to 2, and n is more than or equal to 2; m can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., n can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., and the number of m and n can be designed according to practical needs. The adjustable spacing between adjacent magnetic sleeves 422 may be achieved by telescoping rods.
Specifically, three rows of magnetic bars 421 are disposed on the first support 423, the three rows of magnetic bars 421 are disposed at equal intervals, and each row has 8 magnetic bars 421,8 magnetic bars 421 disposed at equal intervals;
the second support 424 is provided with three rows of magnetic rod sleeves 422, the magnetic rod sleeves 422 in three rows are arranged at equal intervals, and each row is provided with 8 magnetic rod sleeves 422,8 magnetic rod sleeves 422 at equal intervals.
Three sets of the porous deep hole plates 41 may be placed under the magnetic bar frame assembly.
Further, when the sample treatment is large, the number of the magnetic bar frame components can be multiple, and the number of the magnetic bar frame components can be set according to actual needs.
In the present application, as shown in fig. 3-4, the drive assembly further comprises a first drive assembly, a second drive assembly, a third drive assembly and a fourth drive assembly,
the first driving component is used for driving the first bracket to do lifting motion above the porous deep hole plate 41, namely to move up and down along the z-axis; that is, the first driving assembly may drive the magnetic rod 421 to move up and down above or in the porous deep hole plate 41 through the first bracket.
The second driving component is used for driving the second bracket to do lifting motion above the porous deep hole plate 41, namely to move up and down along the z-axis; that is, the second driving assembly may drive the magnetic rod sleeve to make lifting movement above or in the porous deep hole plate 41 through the second bracket.
The third driving assembly is configured to drive the first support and the second support to reciprocate in a first direction, i.e. along a y-axis, relative to the fourth support 44 above the porous deep hole plate 41; that is, the third driving assembly may drive the magnetic rod 421 and the magnetic rod sleeve to move along the y-axis relative to the fourth bracket 44 through the first bracket and the second bracket.
The fourth driving component is used for driving the heating component and the porous deep hole plate 41 to make reciprocating motion along the second direction, namely along the x-axis, on the fourth bracket 44; the first direction is perpendicular to the second direction, i.e. the x-axis direction is perpendicular to the y-axis direction.
In this application, the first driving assembly and the third driving assembly operate simultaneously to drive the first bracket to do circular motion, so as to drive the magnetic rod 421 to do circular motion.
The second driving assembly and the third driving assembly run simultaneously to drive the second bracket to do circular motion, so that the magnetic rod sleeve is driven to do circular motion.
In the present application, the first driving assembly includes a first motor 45, a first sliding rod, and a first sliding block 425, where the first motor 45 drives the first sliding block 425 to move on the first sliding rod; the first slider 425 is fixedly connected to the first bracket. The first sliding rod is perpendicular to the first support.
When the magnetic bar 421 is required to lift, the first motor 45 drives the first slider 425, so that the first slider 425 moves up and down on the first slide bar, and further drives the first bracket and the magnetic bar 421 to move up and down.
In this application, the second driving assembly includes a second motor 46, a second sliding rod, and a second sliding block 426, where the second motor 46 drives the second sliding block 426 to move on the second sliding rod; the second slider 426 is fixedly connected with the second bracket. The second slide bar is arranged perpendicular to the second support.
When the magnetic rod sleeve is required to be lifted, the second motor 46 drives the second slider 426, so that the second slider 426 is lifted on the second slide bar, and further drives the second bracket and the magnetic rod sleeve to lift.
In this application, processing mechanism still includes third support 43, third support 43 is perpendicular with first support and second support, just first slide bar and second slide bar set firmly in on the third support 43, just first slide bar with second slide bar parallel arrangement.
The third driving assembly comprises a third motor 47, a third sliding rod and a third sliding block, and the third motor 47 drives the third sliding block to move on the third sliding rod; the third slider is fixedly connected with the third bracket 43. The third slide bar is fixedly connected with the fourth bracket 44. When the third driving assembly moves, the third motor 47 drives the third slider to move along the third sliding rod (i.e. move along the y-axis), and the third bracket 43 moves along the y-axis along with the third slider, so as to drive the first slider 425 and the second slider 426 to move along the y-axis, and further drive the first bracket, the second bracket, the magnetic rod 421 and the magnetic rod sleeve to move along the y-axis, i.e. the magnetic rod 421 and the magnetic rod sleeve to move between different holes of the porous deep hole plate 41, for example, from the first hole to the second hole or from the second hole to the first hole. When the first driving assembly and the third driving assembly operate simultaneously, the magnetic bar 421 moves up and down and moves left and right, i.e. moves in a circle. When the second driving assembly and the third driving assembly operate simultaneously, the magnetic rod sleeve can move up and down and move left and right in the porous deep hole plate 41, namely, do circular movement, so that the liquid in the porous deep hole plate 41 is uniformly stirred.
In this application, the fourth driving assembly includes a fourth motor 48, a fourth sliding rod and a fourth sliding block 49, where the fourth motor 48 drives the fourth sliding block 49 to move on the fourth sliding rod. The fourth slide bar is perpendicular to the third slide bar, and the fourth slide bar and the third slide bar are fixedly arranged on the same side face of the fourth bracket 44. When a reagent or other conditions need to be injected into the porous deep hole plate 41, the fourth motor 48 drives the fourth sliding block 49 to move along the fourth sliding rod, so as to drive the porous deep hole plate 41 to move along the x axis.
In the present application, the porous deep hole plate 41 has a first hole site, a second hole site and an nth hole site that are sequentially arranged, wherein N is greater than or equal to 3, and the first hole site, the second hole site and the nth hole site each have a plurality of deep holes that are arranged at equal intervals;
the first hole site is used for primarily treating the liquid to be treated so as to obtain a sample to be treated;
the other hole sites between the first hole site and the N-th hole site are used for washing the sample to be treated for multiple times and converting sulfite;
and the N hole site is used for eluting the sample to be treated, so as to obtain a detection sample.
N may be 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, etc., and the number of N may be designed according to actual needs.
The first hole site, the second hole site and the N-th hole site are all provided with M deep holes which are arranged at equal intervals, M is more than or equal to 1, M can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and the like, and the number of M can be determined according to actual needs. The apparatus can thus process M samples simultaneously.
The capacity of the medium deep hole in the first hole site is larger than that of the medium deep holes in other hole sites. The capacity of the deep holes in the same hole site may be the same.
The cross sections of the deep holes of the first hole site, the second hole site and the N-th hole site can be geometric figures such as circles, ellipses, concentric circles, rectangles, triangles, pentagons, hexagons and the like.
The deep holes of the first hole site can be the same as the deep holes of other hole sites or can be different.
The heating assembly comprises at least two mutually independent heating units; each heating unit is provided with a plurality of heating holes, and each heating unit can be independently heated without mutual influence; each heating unit is used for heating the liquid in the corresponding hole site in the porous deep hole plate. The number of the heating holes is the same as the number of the deep holes of the porous deep hole plate 41.
Specifically, when N is 7, the porous deep hole plate 41 includes a first hole site, a second hole site, a third hole site, a fourth hole site, a fifth hole site, a sixth hole site, and a seventh hole site that are sequentially arranged. The first hole site, the second hole site, the third hole site, the fourth hole site, the fifth hole site, the sixth hole site and the seventh hole site are all provided with eight deep holes which are arranged at equal intervals, the capacity of the deep holes in the first hole site is 0-20ml, and the deep holes do not comprise 0, for example, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml, 10ml, 11ml, 12ml, 13ml, 14ml, 15ml, 16ml, 17ml, 18ml, 19ml or 20ml, the capacity of the deep holes in the other hole sites is 0-15ml, the deep holes in the other hole sites do not comprise 0, for example, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml, 10ml, 11ml, 12ml, 13ml, 14ml or 15ml, so that each group of the deep hole plates 41 can process at least 8 human samples, and the deep holes in the first hole site can process at least 10ml plasma at one time.
The first hole site is used for primarily treating the liquid to be treated so as to obtain a sample to be treated;
the second hole site is used for washing a sample to be treated;
the third hole site is used for eluting a sample to be treated and converting sulfite;
The fourth hole site, the fifth hole site and the sixth hole site are sequentially used for washing the sample to be treated after sulfite conversion for multiple times, and a detection sample is obtained in the seventh hole site;
the seventh hole site is used for eluting the sample to be treated, thereby obtaining a detection sample.
In another embodiment, when N is 7, the porous deep hole plate 41 includes a first hole site, a second hole site, a third hole site, a fourth hole site, a fifth hole site, a sixth hole site, and a seventh hole site that are sequentially arranged. The first hole site, the second hole site, the third hole site, the fourth hole site, the fifth hole site, the sixth hole site and the seventh hole site are all provided with 10 deep holes which are arranged at equal intervals, so that 10 different liquids to be treated can be treated simultaneously.
Specifically, the heating component is provided with two heating units, and the two heating units are respectively positioned below the third hole site and the seventh hole site and are used for heating the liquid in the third hole site and the seventh hole site.
The liquid to be treated is one of blood plasma, urine or epidermal tissue, the epidermal tissue can be an epidermal cell, the detection sample to be treated is DNA containing impurities, and the detection sample is DNA.
In this application, the apparatus further comprises a sample zone comprising a liquid storage zone to be treated and a reagent storage zone. The liquid storage area to be treated stores plasma, and the reagent storage area can be used for storing reagents such as magnetic beads, lysate, washing liquid A (washing liquid A is a conventional washing liquid in the prior art), washing liquid B (washing liquid B is a conventional washing liquid in the prior art), eluent (the eluent is a conventional eluent in the prior art) and the like.
The liquid storage area to be treated comprises a sample carrier 7, a sample carrier seat 5 and liquid sample identification equipment to be treated. The sample carrier 7 is located on the sample carrier seat 5, and the sample carrier 7 is provided with a plurality of sample grooves for placing sampling tubes for storing plasma samples. The reagent storage area comprises a reagent tank carrier 8, an eight-connecting-tube carrier 9, a refrigerating module 10 and the like, wherein a plurality of reagent tanks are arranged on the reagent tank carrier 8 and used for storing required reagents, an eight-connecting-tube or 96-hole PCR deep hole plate is arranged on the eight-connecting-tube carrier 9 and used for configuring a PCR system. The refrigeration module 10 is used for storing reagents, samples, etc. at low temperatures.
The liquid to be treated in the liquid storage area is identified and distinguished by the liquid sample to be treated identifying device, the liquid sample to be treated identifying device can be a bar code scanner 12, the bar code scanner 12 is connected with the control mechanism 2, the bar code scanner 12 is used for scanning a bar code on the surface of the sampling tube, information of a sample is obtained by scanning the bar code, and the sample information is sent to the display mechanism 1.
In this application, the sampling mechanism 3 includes a mechanical arm and a pipette, where the mechanical arm is connected to the control mechanism 2, and the mechanical arm is used to control the pipette to add a reagent or a liquid to be treated into the porous deep-well plate 41. The mechanical arm can be connected with a grabbing arm or a pipetting arm.
In a specific embodiment, the sampling mechanism 3 further includes a liquid transferring pump, the liquid transferring pump is connected with the liquid transferring gun, and the liquid transferring pump drives the liquid transferring gun to perform liquid transferring or liquid spitting.
The sampling mechanism 3 further comprises a gun head frame and a waste storage mechanism, wherein the waste storage mechanism can be a waste gun head groove, the gun head frame is used for placing gun heads of a pipetting gun, and the waste gun head groove 6 is used for storing the used gun heads.
In this application, the apparatus further comprises a display mechanism 1, the display mechanism 1 is connected with the control mechanism 2, and the display mechanism 1 is used for giving an instruction to the control mechanism 2, so that the processing mechanism 4, the sample adding mechanism 3 and the PCR reaction plate preparing mechanism are controlled by the control mechanism 2.
In this application, the apparatus further comprises a stage, and the control mechanism 2, the sampling mechanism 3, the processing mechanism 4, the display mechanism 1, and the sample area are all disposed on an upper surface of the stage.
The side of objective table has drawer 11 for deposit the required article of experiment or deposit experiment abandonment consumptive material, the lower surface of objective table is close to the tip and has at least one recess, have moving part in the recess, through moving part is convenient for remove the instrument.
The moving part may be a handle, a lifting lever 13 or the like.
In a specific embodiment, the lower surface of the objective table is rectangular, two grooves parallel to the long sides of the rectangle are formed in the lower surface of the objective table, the lengths of the grooves are equal to those of the long sides, lifting rods 13 are arranged in the two grooves, the length of each lifting rod 13 is greater than that of the corresponding groove, the lifting rods 13 extend out of the corresponding groove, and when the objective table is moved, the lifting rods 13 can be manually grasped to move. The lifting rod 13 may be a telescopic rod, and the lifting rod 13 is retracted in the groove without moving the stage.
In this application, the device further comprises a multifunctional housing assembly comprising an ultraviolet disinfection unit, a lighting unit and an operation warning unit.
The present application also provides a treatment mechanism that is identical to the treatment mechanism in the aforementioned fully automated nucleic acid methylation treatment apparatus, and thus the treatment mechanism can refer to the description of the treatment mechanism in the aforementioned fully automated nucleic acid methylation treatment apparatus.
The application also provides full-automatic nucleic acid methylation analysis equipment, which comprises the full-automatic nucleic acid methylation processing equipment and one of a QPCR detection platform, an NGS detection platform or a FISH detection platform.
When the full-automatic nucleic acid methylation treatment equipment is used, firstly, the bar code scanner is adopted to scan the bar code on the sampling tube, the bar code scanner transmits acquired information to the display mechanism, then plasma in the sampling tube is transferred to the first hole site through the sampling mechanism 3, then required reagent is transferred to the first hole site through the sampling mechanism 3, the first driving assembly drives the magnetic rod 421 to stretch into the magnetic rod sleeve 422, the positions of the magnetic rod 421 and the magnetic rod sleeve 422 are the first positions, the second driving assembly drives the magnetic rod sleeve 422 to descend to stretch into the first hole site, then the second driving assembly and the third driving assembly simultaneously operate to drive the magnetic rod sleeve 422 to make circular motion in the porous deep hole plate 41, and then the plasma and the pyrolysis are uniformly mixed, so that DNA is cracked from the plasma, and the pyrolysis is completed. The third drive assembly then stops and the second drive assembly brings the bar magnet sleeve 422 back to the first position. Then the fourth driving component drives the porous deep hole plate 41 to move along the x axis, so as to move in a direction away from the magnetic rod 421 and the magnetic rod sleeve, the magnetic beads are transferred from the sample area to the first hole site through the sample adding mechanism 3, then the anhydrous ethanol is added into the first hole site through the sample adding mechanism 3 from the sample area, then the fourth driving component moves to drive the porous deep hole plate 41 to move along the x axis and restore to the original position, then the second driving component drives the magnetic rod sleeve 422 to descend and stretch into the first hole site, and then the second driving component and the third driving component simultaneously operate so as to drive the magnetic rod sleeve 422 to move in the porous deep hole plate 41, and the first combination is completed. Then the third driving assembly stops running, and the second driving assembly drives the magnetic rod sleeve 422 to restore to the first position; then the fourth driving component drives the porous deep hole plate 41 to move along the x axis, so as to move in a direction away from the magnetic rod 421 and the magnetic rod sleeve, the sampling mechanism 3 sucks the washing liquid A from the reagent area, adds the washing liquid A into the second hole site, and then the fourth driving component moves to drive the porous deep hole plate 41 to move along the x axis and restore to the original position; the first driving component and the second driving component simultaneously drive the magnetic rod 421 and the magnetic rod sleeve 422 to descend into the first hole site, the magnetic beads adsorbed with DNA are adsorbed on the outer wall of the magnetic rod sleeve 422, the first driving component and the second driving component drive the magnetic frame and the magnetic rod sleeve 422 to ascend to a first position, then the first driving component and the second driving component stop running, the third driving component is started, the magnetic rod 421 and the magnetic rod sleeve are driven by the third driving component to move along the y axis, namely move towards the direction of the second hole site, when the magnetic rod 421 reaches the position right above the second hole site, the first driving component and the second driving component drive the magnetic beads to stretch into the second hole site, then the first driving component drives the magnetic rod 421 to ascend, when the magnetic rod 421 ascends to a certain height, the magnetic beads adsorbed with DNA on the outer wall of the magnetic rod sleeve 422 automatically fall into the second hole site, the second driving component drives the second hole site to move along the y axis, and the second driving component drives the second hole site to reciprocate in the second hole site, and the second hole site is driven by the second driving component to reciprocate to the second hole site, and the second hole site is washed by the second vibration. Then the fourth driving component drives the porous deep hole plate 41 to move along the x axis, so as to move towards the direction far away from the magnetic rod 421 and the magnetic rod sleeve, the sampling mechanism 3 sucks eluent from the reagent area, adds the eluent into the third hole site, after the heating unit below the third hole site heats up until reaching a specified temperature, the fourth driving component moves to drive the porous deep hole plate 41 to move along the x axis, and returns to the original position, the third driving component drives the magnetic rod 421 and the magnetic rod sleeve to move along the y axis, namely move towards the direction where the third hole site is located, when reaching the position right above the third hole site, the first driving component and the second driving component drive the magnetic beads to stretch into the third hole site, then the first driving component drives the magnetic rod to rise, when the magnetic rod 421 rises to a certain height, the magnetic beads with DNA adsorbed on the outer wall of the magnetic rod sleeve 422 automatically fall into the third hole site, and the second driving component drives the magnetic rod sleeve 422 to move along the y axis, so that the magnetic rod 421 can reciprocate in the porous deep hole plate 41, and the liquid is completely and linearly eluted in the third hole site. The first driving assembly drives the magnetic rod 421 to extend into the magnetic rod sleeve 422, the magnetic beads are adsorbed on the outer wall of the magnetic rod sleeve 422, the first driving assembly and the second driving assembly drive the magnetic rod 421 and the magnetic rod sleeve 422 to ascend, then the third driving assembly moves along the y-axis direction, namely, moves towards the direction where the second hole site is located, and then the magnetic beads are discarded in the second hole site. And the sampling mechanism 3 respectively absorbs sulfite and protective solution from the reagent area, both the sulfite and the protective solution are added into a third hole site, the second driving assembly drives the magnetic rod sleeve 422 to extend into the third hole site after the heating unit is heated to a specified temperature, the magnetic rod sleeve 422 does linear reciprocating motion along the y axis in the third hole site, the liquid in the magnetic rod sleeve 422 oscillates, and the sulfite conversion is completed after the specified time of standing. The sampling mechanism 3 sucks the washing liquid A from the reagent area, adds the washing liquid A into the third hole site, meanwhile, the sampling mechanism 3 sucks new magnetic beads from the reagent area and adds the new magnetic beads into the third hole site, after the heating unit is heated to a specified temperature, the second driving component drives the magnetic rod sleeve 422 to enter the liquid in the third hole site to oscillate, and after the oscillation is finished, the second combination is finished. And transferring the DNA adsorbed with the magnetic beads into the fourth hole site through the magnetic rod rack assembly, and adopting a washing liquid B to finish the second washing. Transferring the DNA adsorbed with the magnetic beads into the fifth hole site through the magnetic rod rack assembly, and finishing the third washing by adopting the washing liquid B. And transferring the DNA adsorbed with the magnetic beads into the sixth hole site through the magnetic rod rack assembly, and finishing the fourth washing by adopting the washing liquid B. And transferring the DNA adsorbed with the magnetic beads into the seventh hole site through the magnetic rod rack assembly, heating a heating unit below the seventh hole site to a specified temperature, and performing second elution by using eluent to obtain free DNA. And then discarding the magnetic beads into the fifth hole site by using the magnetic rod frame assembly. The sample loading mechanism 3 then transfers the liquid in the seventh well to the PCR reaction plate of the PCR reaction plate preparing mechanism. The sampling mechanism 3 completes the configuration of the PCR pre-reaction liquid at the PCR reaction plate preparation mechanism according to the proportion. Then, the sample adding mechanism 3 sucks the PCR pre-reaction solution from the PCR reaction plate preparing mechanism (low temperature reagent zone), transfers the solution into the PCR reaction plate, and after the PCR reaction system is constructed, the control mechanism 2 sends the data to the display mechanism 1 last time, extracts DNA from blood plasma, and carries out PCR methylation treatment. And the control mechanism 2 controls the sampling mechanism 3, the processing mechanism 4, the sampling mechanism 3 and the PCR reaction plate preparation mechanism to restore to the original positions.
Therefore, the device of the application realizes the whole process from the sample entering the device to the completion of obtaining the target DNA extraction, does not need manual intervention, can realize the high automation of nucleic acid methylation extraction, can save labor compared with the prior art, can process 96 samples a day, greatly saves labor, reduces manual errors, saves hundreds of uncaps and liquid transfer, standardizes the process, simultaneously greatly saves time, and reduces the processing time of 48 samples from 7 hours to 4.5 hours. The devices described herein can detect multiple types of samples, such as blood, urine, exfoliative cells, and the like.
Although embodiments of the present application have been described above with reference to the accompanying drawings, the present application is not limited to the specific embodiments and fields of application described above, which are merely illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may make numerous forms, and equivalents thereof, without departing from the scope of the invention as defined by the claims.
Claims (10)
1. A full-automatic nucleic acid methylation processing device is characterized by comprising a control mechanism, a sample adding mechanism and a processing mechanism, wherein the control mechanism respectively controls the sample adding mechanism and the processing mechanism,
The processing mechanism comprises a plurality of groups of parallel porous deep pore plates, a magnetic rod rack assembly, a driving assembly and a heating assembly,
the magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves which are arranged in an array manner, wherein the magnetic rods are positioned above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves;
the magnetic rod rack assembly is positioned above the porous deep pore plate, the magnetic rod rack assembly or the magnetic rod sleeve is driven by the driving assembly to move above the porous deep pore plate, and the magnetic rod rack assembly or the magnetic rod sleeve can move downwards to extend into the porous deep pore plate;
the heating component is positioned below the porous deep pore plate and is used for heating liquid in the porous deep pore plate.
2. The fully automatic nucleic acid methylation processing apparatus of claim 1, wherein the driving assembly drives the magnetic rod rack assembly or the magnetic rod sleeve to extend into the porous deep pore plate and make circular movement or linear reciprocating movement for oscillating and uniformly mixing the liquid in the porous deep pore plate.
3. The fully automated nucleic acid methylation processing apparatus of claim 2, wherein the processing mechanism further comprises a first rack and a second rack, the second rack being located directly below the first rack;
The processing mechanism further comprises a fourth bracket, the heating component is movably arranged on the fourth bracket, and the heating component is fixedly connected with the porous deep pore plate;
the first bracket, the second bracket and the fourth bracket are mutually parallel.
4. The fully automatic nucleic acid methylation processing apparatus according to claim 3,
m rows of magnetic bars are arranged on the first support, the distance between the m rows of magnetic bars is adjustable, each row of magnetic bars is provided with n magnetic bars, and the distance between the n magnetic bars is adjustable;
m rows of magnetic rod sleeves are arranged on the second bracket, the distance between the m rows of magnetic rod sleeves is adjustable, each row is provided with n magnetic rod sleeves, the distance between the n magnetic rod sleeves is adjustable,
wherein m is greater than or equal to 2, and n is greater than or equal to 2.
5. The apparatus according to claim 3, wherein the driving means further comprises a first driving means, a second driving means, a third driving means and a fourth driving means,
the first driving component is used for driving the first bracket to do lifting motion above the porous deep hole plate;
the second driving component is used for driving the second bracket to do lifting motion above the porous deep hole plate;
The third driving assembly is used for driving the first bracket and the second bracket to do reciprocating motion relative to the fourth bracket along a first direction above the porous deep hole plate;
the fourth driving component is used for driving the heating component and the porous deep pore plate to make reciprocating motion on the fourth bracket along a second direction;
the first direction is perpendicular to the second direction.
6. The apparatus according to claim 5, wherein the first driving assembly and the third driving assembly are operated simultaneously to drive the first support to perform a circular movement, thereby driving the magnetic bar to perform a circular movement;
the second driving assembly and the third driving assembly run simultaneously to drive the second bracket to do circular motion, so that the magnetic rod sleeve is driven to do circular motion.
7. The fully automatic nucleic acid methylation processing apparatus according to claim 5,
the first driving assembly comprises a first motor, a first sliding rod and a first sliding block, and the first motor drives the first sliding block to move on the first sliding rod;
the first sliding block is fixedly connected with the first bracket.
8. The fully automatic nucleic acid methylation processing apparatus according to claim 5,
the second driving assembly comprises a second motor, a second sliding rod and a second sliding block, and the second motor drives the second sliding block to move on the second sliding rod;
the second sliding block is fixedly connected with the second bracket.
9. The fully automated nucleic acid methylation processing apparatus of claim 8, wherein the processing mechanism further comprises a third support, the third support is perpendicular to the first support and the second support, the first slide bar is parallel to the second slide bar, and the first slide bar and the second slide bar are fixedly arranged on the third support;
the first sliding rod and the second sliding rod are perpendicular to the first bracket and the second bracket.
10. A processing mechanism is characterized by comprising a plurality of groups of parallel porous deep hole plates, a magnetic rod rack assembly, a driving assembly and a heating assembly,
the magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves which are arranged in an array manner, wherein the magnetic rods are positioned above the magnetic rod sleeves and can move downwards to extend into the magnetic rod sleeves;
the magnetic rod rack assembly is positioned above the porous deep pore plate, the magnetic rod rack assembly or the magnetic rod sleeve is driven by the driving assembly to move above the porous deep pore plate, and the magnetic rod rack assembly or the magnetic rod sleeve can move downwards to extend into the porous deep pore plate;
The heating component is positioned below the porous deep pore plate and is used for heating liquid in the porous deep pore plate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111675068.6A CN116410842A (en) | 2021-12-31 | 2021-12-31 | Full-automatic nucleic acid methylation treatment equipment and treatment mechanism |
| PCT/CN2022/071992 WO2023123571A1 (en) | 2021-12-31 | 2022-01-14 | Fully-automatic nucleic acid methylation treatment device and treatment mechanism |
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| CN202111675068.6A CN116410842A (en) | 2021-12-31 | 2021-12-31 | Full-automatic nucleic acid methylation treatment equipment and treatment mechanism |
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| WO (1) | WO2023123571A1 (en) |
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| CN116751674A (en) * | 2023-08-22 | 2023-09-15 | 广州凯普医药科技有限公司 | Magnetic bead method DNA draws and DNA methylation conversion purification device |
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| GB0717452D0 (en) * | 2007-09-07 | 2007-10-17 | Mole Genetics As | Separation apparatus |
| CN103923162B (en) * | 2013-01-15 | 2016-08-10 | 常州金麦格生物技术有限公司 | Vibrating mechanism for bar magnet method bioactive substance extraction apparatus and application thereof |
| CN103789198B (en) * | 2014-02-27 | 2015-09-16 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acid |
| CN104059848A (en) * | 2014-06-26 | 2014-09-24 | 广州冠科生物科技有限公司 | Uniform mixing device for nucleic acid extraction |
| CN104977349B (en) * | 2015-03-17 | 2019-04-23 | 北京毅新博创生物科技有限公司 | Full-automatic polypeptide extracts flight time mass spectrum detector |
| CN108048302B (en) * | 2018-02-01 | 2024-03-19 | 北京同立创辉仪器有限公司 | Full-automatic nucleic acid extractor |
| CN108949505B (en) * | 2018-08-15 | 2024-04-02 | 烟台艾德康生物科技有限公司 | Nucleic acid extraction device and method |
| CN211112037U (en) * | 2019-08-29 | 2020-07-28 | 中国科学院苏州生物医学工程技术研究所 | High-flux nucleic acid extraction system |
| CN111004797B (en) * | 2019-12-06 | 2021-07-30 | 深圳市宝安区沙井人民医院 | Nucleic acid extraction device and nucleic acid extraction method |
| CN112608829B (en) * | 2019-12-16 | 2023-01-31 | 博尔诚（北京）科技有限公司 | Full-automatic nucleic acid methylation amplification detection equipment |
| CN113846009A (en) * | 2021-08-19 | 2021-12-28 | 韦玉军 | Liquid separating extractor by magnetic bead method |
-
2021
- 2021-12-31 CN CN202111675068.6A patent/CN116410842A/en active Pending
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| CN116751674A (en) * | 2023-08-22 | 2023-09-15 | 广州凯普医药科技有限公司 | Magnetic bead method DNA draws and DNA methylation conversion purification device |
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