CN113198360A - Vibrating magnetic equipment and vibrating magnetic attraction method for biological sample - Google Patents
Vibrating magnetic equipment and vibrating magnetic attraction method for biological sample Download PDFInfo
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- CN113198360A CN113198360A CN202110486158.4A CN202110486158A CN113198360A CN 113198360 A CN113198360 A CN 113198360A CN 202110486158 A CN202110486158 A CN 202110486158A CN 113198360 A CN113198360 A CN 113198360A
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- 239000012472 biological sample Substances 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000000523 sample Substances 0.000 claims abstract description 58
- 230000007246 mechanism Effects 0.000 claims abstract description 56
- 230000006698 induction Effects 0.000 claims abstract description 51
- 230000003044 adaptive effect Effects 0.000 claims abstract description 18
- 230000010355 oscillation Effects 0.000 claims description 38
- 238000005096 rolling process Methods 0.000 claims description 14
- 230000005389 magnetism Effects 0.000 claims description 11
- 239000011324 bead Substances 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 4
- 230000006978 adaptation Effects 0.000 abstract description 6
- 238000000926 separation method Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 230000003028 elevating effect Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 230000004044 response Effects 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 description 9
- 230000001174 ascending effect Effects 0.000 description 6
- 210000004907 gland Anatomy 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/60—Mixers with shaking, oscillating, or vibrating mechanisms with a vibrating receptacle
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/05—Mixers using radiation, e.g. magnetic fields or microwaves to mix the material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
- B01F33/836—Mixing plants; Combinations of mixers combining mixing with other treatments
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
- B01F35/2216—Time, i.e. duration, of at least one parameter during the operation
- B01F35/22161—Time, i.e. duration, of at least one parameter during the operation duration of the mixing process or parts of it
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a vibration magnetic device and a biological sample vibration magnetic attraction method in the field of vibration separation devices. The vibrating magnetic equipment comprises a base, wherein a vibrating mechanism, a lifting mechanism, a magnetic device and a vibrating adaptive seat for installing a sample seat are arranged on the base, and the vibrating mechanism is connected with the vibrating adaptive seat and drives the vibrating adaptive seat to vibrate; the lifting mechanism is connected with the magnetic device and drives the magnetic device to lift, the magnetic device forms a magnetic field and rises to act on the sample seat, one of the base and the oscillating mechanism is provided with an origin induction sensor, and the other is provided with an origin induction sheet used for triggering the origin induction sensor. The beneficial effects are that: vibrate mechanism, elevating system, magnetic means are integrated on same base, have reduced occupation space, and experiment easy operation is convenient to realize vibrating the location of adaptation seat through initial point inductive transducer and initial point response piece cooperation, guaranteed magnetic means and the counterpoint precision of vibrating the adaptation seat, be favorable to improving the experimental quality.
Description
Technical Field
The invention relates to the field of vibration separation equipment, in particular to vibration magnetic equipment and a vibration magnetic absorption method for a biological sample.
Background
Magnetic bead separation is used as a common separation method for separating samples such as nucleic acid, protein or cells, vibration and magnetism absorption steps are involved in the separation experiment process, and the vibration and magnetism absorption steps are generally respectively arranged on different stations of a common automatic liquid transfer workstation, so that the occupied space is large. At present, some devices integrating vibration and magnetism absorption are also arranged, but the devices are complex in structure and low in positioning precision, and the experimental quality of a sample is difficult to guarantee.
Disclosure of Invention
In view of the above disadvantages of the prior art, the present invention provides an oscillating magnetic device and an oscillating magnetic method for biological samples, which are used to solve the problems of large occupied space and low experimental quality of the separation device for biological and chemical substances in the prior art.
In order to achieve the above and other related objects, the present invention provides an oscillating magnetic device, comprising a base, wherein the base is provided with an oscillating mechanism, a lifting mechanism, a magnetic device and an oscillating adapter seat for mounting a sample seat, the oscillating mechanism is connected with the oscillating adapter seat and drives the oscillating adapter seat to oscillate; the lifting mechanism is connected with the magnetic device and drives the magnetic device to lift, the magnetic device forms a magnetic field and rises to act on the sample seat, one of the base and the oscillating mechanism is provided with an origin induction sensor, and the other is provided with an origin induction sheet used for triggering the origin induction sensor.
The invention has the beneficial effects that: vibrate mechanism, elevating system, magnetic means are integrated on same base, have reduced occupation space, and experiment easy operation is convenient to realize vibrating the location of adaptation seat through initial point inductive transducer and initial point response piece cooperation, guaranteed magnetic means and the counterpoint precision of vibrating the adaptation seat, be favorable to improving the experimental quality.
Optionally, the oscillation mechanism comprises an oscillation motor and a rolling bearing assembly, the rolling bearing assembly comprises a rotating shaft, a bearing outer sleeve and a rolling bearing arranged in the bearing outer sleeve, the bearing outer sleeve is connected with the oscillation adaptive seat, an upper shoulder connected with the rolling bearing is arranged at the upper end of the rotating shaft, a lower shoulder connected with the oscillation motor is arranged at the lower end of the rotating shaft, and the upper shoulder and the lower shoulder are eccentrically arranged.
Optionally, the lifting mechanism comprises a lifting motor, a lifting screw is installed at the output end of the lifting motor, and a lifting nut matched with the lifting screw is installed on the magnetic device.
Optionally, a vibration connecting plate is installed at the bottom of the vibration adapting seat, a positioning hole is formed in the vibration connecting plate, a positioning structure matched with the positioning hole is installed on the magnetic device, and when the magnetic device ascends, the positioning structure is driven to ascend and be matched with the positioning hole for positioning.
Optionally, the positioning structure includes a positioning pin, a lower end of the positioning pin is movably connected with the magnetic device, an upper end of the positioning pin is in positioning fit with the positioning hole, the upper end of the positioning pin is provided with a limiting portion and a spring, and two ends of the spring respectively support against the limiting portion and the magnetic device.
Optionally, the magnetic device includes a magnetic bar rack, and the magnetic bar rack is provided with a magnetic bar group.
Optionally, the magnetic bar group comprises a plurality of magnetic bars with magnetism, and the plurality of magnetic bars are distributed in a matrix; when the magnetic field formed by the magnetic rod acts on the sample tube of the sample holder, the upper end of the magnetic rod is positioned at one side of the lower end of the sample tube.
Optionally, an ascending induction sensor and a descending induction sensor located below the ascending induction sensor are mounted on the base, and a lifting induction sheet for triggering the ascending induction sensor and the descending induction sensor is mounted on the magnetic rod frame.
Optionally, the oscillation magnetic device further comprises an upper computer, a control card is installed on the base, and the upper computer controls the start and stop of the oscillation mechanism and the lifting mechanism through the control card.
The beneficial effect of adopting the above optional scheme is: the magnetic device and the adaptive seat are aligned accurately, so that the magnetic rod can accurately extend into the space between the sample tubes of the sample seat, and the magnetic field effect is ensured.
A biological sample vibrates and attracts the magnetic method, will load the sample seat of the sample into and vibrate the adaptive seat, make the control card control the oscillating mechanism to run in the predetermined time through the upper computer, the oscillating mechanism drives and vibrates the adaptive seat; when the oscillating mechanism finishes the preset oscillating time and the origin induction sheet triggers the origin induction sensor for the first time, the upper computer controls the oscillating mechanism to stop at the origin according to the feedback signal of the origin sensor; when the oscillation mechanism stops operating, the upper computer controls the lifting device to start operating, the lifting device drives the magnetic device to ascend until a magnetic field formed by the magnetic device can act on the magnetic beads in the sample seat, and the magnetic device attracts the magnetic beads in the sample seat; after the magnetism is absorbed, the lifting device drives the magnetic device to descend and keep away from the sample seat.
The invention has the beneficial effects that: the automatic aligning mechanism is simple and convenient to operate, and can automatically return to the original point after the oscillating mechanism completes oscillation, so that the aligning precision and the experimental quality of a sample are guaranteed.
Drawings
FIG. 1 is a schematic structural diagram of a magnetic oscillating apparatus according to the present invention;
FIG. 2 is a top view of the oscillating magnetic device of the present invention;
FIG. 3 shows a cross-sectional view A-A of FIG. 2;
FIG. 4 is a first perspective assembly view of the oscillating magnetic device of the present invention;
FIG. 5 is an enlarged view of portion B of FIG. 4;
fig. 6 is an assembly view of the oscillating magnetic device according to the second aspect of the present invention.
Description of reference numerals
101-a base; 102-a support base; 103-a control card; 104-control card fixing plate; 105-a housing; 106-origin inductive sensor;
201-oscillation adaptation seat; 202-oscillating connecting plates; 203-bearing outer sleeve; 204-rolling bearing; 205-bearing gland; 206-an eccentric shaft; 207-counterweight wheel; 208-oscillating motor fixing plate; 209-oscillating motor; 210-origin sensing piece;
301-magnetic bar; 302-magnetic force bar rack; 303-magnetic frame fixing plate; 304-a lifting nut; 305-a lifting screw; 306-a lift motor; 307-lifting motor fixing plate; 308-lifting induction sheet; 309-rise induction sensor; 310-a descent sensing sensor; 311-a sensor mount; 312-a lift guide shaft; 313-a lifting guide sleeve;
401-locating pins; 402-a spring; 403-a gasket; 404-set screws;
501-sample well plate; 502-orifice plate adapter.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
It should be noted that the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for clarity of description only, and are not intended to limit the scope of the present invention, and changes or modifications of the relative relationship between the terms and the corresponding elements may be made without substantial technical changes.
Before describing embodiments of the present invention in detail, the present invention will be described in an application environment. The technology of the invention is mainly applied to an automatic liquid transfer platform, in particular to the vibration and magnetism absorption of biological samples such as nucleic acid, protein or cells. The invention solves the problems that the traditional biological sample treatment vibration and magnetism absorption need to be carried out by different equipment, the equipment occupies large space, the operation is inconvenient, the sample positioning precision is low, the sample treatment quality is influenced and the like.
As shown in fig. 1 to 6, the oscillating magnetic device of the present invention includes a base 101, wherein the base 101 is mounted with an oscillating mechanism, a lifting mechanism, a magnetic device and an oscillating adapter 105 for mounting a sample holder, the oscillating mechanism is connected with the oscillating adapter 105 and drives the oscillating adapter 105 to oscillate, so that the sample holder mounted in the oscillating adapter 105 and the oscillating adapter oscillate together. Elevating system is connected with magnetic means and drives magnetic means lift, and magnetic means forms magnetic field and rises and act on the sample seat, and base 101 and one of them install origin induction sensor 106 in the oscillation mechanism, and the other installs the origin induction piece 210 that is used for triggering origin induction sensor 106, triggers origin induction sensor 106 through origin induction piece 210 and ensures that the oscillation mechanism whether returns to the origin to ensure positioning accuracy.
As shown in fig. 1-6, in an exemplary embodiment, the magnetic device includes a magnetic bar rack 302, and the magnetic bar rack 302 has a magnetic bar set mounted thereon. The magnetic bar set comprises a plurality of magnetic bars 301 with magnetism, and the plurality of magnetic bars 301 can be distributed in a matrix. The magnetic rod 301 may have a magnetic property at its upper end or the whole magnetic property, when the magnetic field formed by the magnetic rod 301 acts on the sample tube of the sample holder, the upper end of the magnetic rod 301 is located at one side of the lower end of the sample tube, and the axial direction of the magnetic rod 301 is parallel to the axial direction of the sample tube. The sample holder comprises a sample hole plate 501 and a hole plate adapting seat 502, the sample hole plate 501 is installed on the hole plate adapting seat 502, the hole plate adapting seat 502 is installed on the oscillation adapting seat 201, a sample tube is arranged on the sample hole plate 501 in a downward protruding mode, and the sample tube is filled with samples and magnetic beads. The plurality of sample tubes may be arranged in a matrix, and the ends of the sample tubes are staggered from the ends of the magnetic rods 301. By adopting the structure layout, when the magnetic device ascends to reach a working position, the periphery of each sample tube is surrounded by the plurality of magnetic rods 301, each magnetic rod acts on the plurality of sample tubes simultaneously, the stability of magnetic force is ensured, and the phenomenon that the sample tubes cannot finish magnetic attraction due to the fact that the single magnetic rod 301 breaks down is avoided.
As shown in fig. 1 to 6, in an exemplary embodiment, the oscillation mechanism includes an oscillation motor 209 and a rolling bearing assembly, and the oscillation motor 209 is mounted on the base 101 and can be pressed and fixed by an oscillation motor fixing plate 208. The rolling bearing assembly comprises a rolling bearing 204, a bearing outer sleeve 203, a bearing gland 205 and a rotating shaft 206, the rolling bearing 204 is installed in the bearing outer sleeve 203, the bearing gland 205 is connected with the rotating shaft 206 and limits the rolling bearing 204 to be separated from the bearing outer sleeve 203, and the bearing outer sleeve 203 is connected with the oscillation adaptive seat 201 through an oscillation connecting plate 202; the upper end of pivot 206 is equipped with the shaft shoulder, goes up the shaft shoulder and cooperates with antifriction bearing 204, and the lower extreme of pivot 206 is equipped with down the shaft shoulder, and lower shaft shoulder is connected with the output of shock dynamo 209, and the external diameter of going up the shaft shoulder is greater than the external diameter of shaft shoulder down, and goes up the shaft shoulder and set up with lower shaft shoulder off-centre, makes rotatory focus and shock dynamo produce the skew through the last shaft shoulder of off-centre setting and lower shaft shoulder. The lower shaft shoulder is also sleeved with a counterweight wheel 207, and the counterweight wheel, the lower shaft shoulder and the output end of the oscillating motor are fixedly connected through screws; the gravity center during rotation can be transferred through the weight wheel, and during high-speed rotation, the shaking of equipment work can be reduced, and the equipment work is more stable. The oscillating motor drives the oscillating adaptive seat to oscillate along the circumferential direction in the horizontal plane through the eccentric bearing assembly
As shown in fig. 1 to 6, in an exemplary embodiment, the lifting mechanism includes a lifting motor 306, and the lifting motor 306 is mounted on the base 101 and can be pressed and fixed by a lifting motor fixing plate 307. The output end of the lifting motor 306 is provided with a lifting screw 305, and the magnetic device is provided with a lifting nut 302 which is in threaded fit with the lifting screw 305. Wherein, lifting nut 302 can be installed in the bottom of bar magnet frame 302 through magnetic frame fixed plate 303, is equipped with the structure of splining that restricts bar magnet frame 302 pivoted on the bar magnet frame 302, when lifting motor 306 drives lifting screw 305 forward or reverse rotation, just converts lifting screw 305's rotation into lifting nut 302's lift removal to realize magnetic means's elevating movement.
As shown in fig. 1 to 6, in an exemplary embodiment, the oscillating magnetic device further includes a lifting guide shaft 312 and a lifting guide sleeve 313 in guiding fit with the lifting guide shaft 312, one of the lifting guide shaft 312 and the lifting guide sleeve 313 is mounted on the magnetic rod holder 302, and the other is mounted on the base 101. In this embodiment, the elevation guide shaft 312 is installed at the bottom of the magnet bar frame 302, and the elevation guide sleeve 313 is installed on the base. The base 101 is provided with an ascending induction sensor 309 and a descending induction sensor 310 located below the ascending induction sensor 309, and the ascending induction sensor 309 and the descending induction sensor 310 can be mounted on a support base of the base through a sensor fixing base 311. The magnetic bar frame 302 is provided with a lifting induction sheet 308 for triggering a lifting induction sensor 309 and a falling induction sensor 310. When the magnetic bar rack 302 moves upwards, the lifting induction sheet 308 triggers the lifting induction sensor 309, and the upper computer enables the control card to control the lifting motor to stop running according to a feedback signal of the lifting induction sensor; when the magnetic bar rack 302 moves downwards, the lifting induction sheet 308 triggers the lifting induction sensor 310, and the upper computer enables the control card to control the lifting motor to stop running according to the feedback signal of the lifting induction sensor.
As shown in fig. 1 to 6, in an exemplary embodiment, an oscillating connecting plate 202 is mounted at the bottom of the oscillating adapter seat 105. Be equipped with the locating hole on shaking connecting plate 202, install on the magnetic means and shake connecting plate 202 and correspond direction complex location structure, when the magnetic means rose, drive location structure and go upward and shake the locating hole cooperation location on the connecting plate 202. In this embodiment, the positioning structure can be installed on the magnetic rack fixing plate 303, and the positioning structures are installed on the magnetic rack fixing plates located at the two ends of the bottom of the magnetic rod rack 302, so as to improve the positioning accuracy of the magnetic rod rack and the oscillation adapting seat.
As shown in fig. 1 to 6, in an exemplary embodiment, the positioning structure includes a positioning pin 401, and an upper end surface of the positioning pin 401 may be a tapered surface to facilitate guiding positioning. The lower extreme of locating pin 401 and magnetic force frame fixed plate 303 swing joint of magnetic means, the upper end and the locating hole location fit of locating pin 401, the upper end of locating pin 401 is equipped with spacing portion and cover and is equipped with spring 402, the both ends of spring 402 withstand spacing portion and magnetic force frame fixed plate 303 of magnetic means respectively, spring 402 is in compression state, magnetic force frame fixed plate 303 can adopt L type or Z type structure. The lower end of the positioning pin 401 passes through the through hole of the magnetic frame fixing plate, and the positioning pin 401 is limited to move upwards to be separated from the magnetic frame fixing plate 303 by a fixing screw 404 and a gasket 403 which are arranged at the end part of the positioning pin 401. By adopting the structural design, the automatic resetting of the positioning pin can be realized, the connecting plate can be prevented from being vibrated by the transition and jacking of the positioning pin, the operation is flexible and stable, and the damage to parts is avoided. When the magnetic rod rack 302 drives the positioning pin 401 to move upwards, the positioning pin 401 extends into the positioning hole to drive the oscillation adapting seat 105 to be precisely adjusted to the original point, so that the precise positioning of the magnetic device and the oscillation adapting seat is ensured.
As shown in fig. 1 to 6, in an exemplary embodiment, the oscillating magnetic device further includes an upper computer, a control card 103 is installed on the base 101, and the upper computer controls the start and stop of the oscillating mechanism and the lifting mechanism through the control card 103. Wherein, a support seat 102 extending upwards can be arranged on the base 101, and the control card 103 is mounted on the support seat 102 through a control card fixing plate 104.
As shown in fig. 1 to 6, in an exemplary embodiment, the oscillating magnetic device further includes an outer casing 105, a top of the outer casing 105 is open, the base 101, the magnetic device, the lifting mechanism and the oscillating mechanism are installed in the outer casing 105, and the oscillating adapter 201 is located above the lifting mechanism, the magnetic device and the oscillating mechanism and outside the outer casing 105, so as to facilitate the oscillating adapter 201 to oscillate fully along a circumferential direction in a horizontal plane. Vibrate adaptation seat 201 center and vibrate connecting plate center and all be equipped with the hole of dodging that is used for dodging magnetic means lift for the magnetic rod directly stretches into smoothly between each sample cell.
The biological sample oscillation magnetic absorption method comprises the steps that a sample seat provided with a sample is installed in an oscillation adaptive seat, an upper computer enables a control card to control an oscillation mechanism to operate within preset time, and the oscillation mechanism drives the oscillation adaptive seat to oscillate; wherein, the host computer can vibrate the start-up, stop, vibrate time, vibrate rotational speed, vibrate the stop position etc. of mechanism according to demand control, and control operation is simple and convenient, realizes automatic control, reduces the manual operation interference. After the oscillating mechanism finishes the preset oscillating time, when the original point induction sheet triggers the original point induction sensor for the first time, the upper computer controls the oscillating mechanism to stop at the original point according to the feedback signal of the original point sensor. When the oscillation mechanism stops operating, the upper computer controls the lifting device to start operating, the lifting device drives the magnetic device to ascend until a magnetic field formed by the magnetic device can act on the magnetic beads in the sample seat, and the magnetic device attracts the magnetic beads in the sample seat; after the magnetism is absorbed, the lifting device drives the magnetic device to descend to be far away from the sample seat, and the magnetic beads in the sample seat are demagnetized.
The magnetic force oscillation equipment and the biological sample magnetic absorption method realize the combination of sample oscillation and magnetic absorption through a simple structure and an operation method, simplify the equipment structure, reduce the occupied space, realize initial positioning through the matching of the origin induction sensor and the origin induction sheet, realize precise positioning through the matching of the oscillation connecting plate and the positioning pin, and ensure the positioning precision of the magnetic force rod and the sample tube by adopting a step-by-step positioning mode, thereby ensuring the running stability of the equipment and the quality and the efficiency of sample processing.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (10)
1. A vibrate magnetic force equipment which characterized in that: the sample holder comprises a base, wherein a vibration mechanism, a lifting mechanism, a magnetic device and a vibration adaptive seat for mounting a sample holder are arranged on the base, and the vibration mechanism is connected with the vibration adaptive seat and drives the vibration adaptive seat to vibrate; the lifting mechanism is connected with the magnetic device and drives the magnetic device to lift, the magnetic device forms a magnetic field and rises to act on the sample seat, one of the base and the oscillating mechanism is provided with an origin induction sensor, and the other is provided with an origin induction sheet used for triggering the origin induction sensor.
2. The oscillating magnetic force apparatus of claim 1, wherein: the vibration mechanism comprises a vibration motor and a rolling bearing assembly, the rolling bearing assembly comprises a rotating shaft, a bearing outer sleeve and a rolling bearing arranged in the bearing outer sleeve, the bearing outer sleeve is connected with a vibration adaptive seat, an upper shaft shoulder connected with the rolling bearing is arranged at the upper end of the rotating shaft, a lower shaft shoulder connected with the vibration motor is arranged at the lower end of the rotating shaft, and the upper shaft shoulder and the lower shaft shoulder are eccentrically arranged.
3. The oscillating magnetic force apparatus of claim 1, wherein: the lifting mechanism comprises a lifting motor, a lifting screw is installed at the output end of the lifting motor, and a lifting nut matched with the lifting screw is installed on the magnetic device.
4. The oscillating magnetic force apparatus of claim 1, wherein: the bottom of the oscillation adaptive seat is provided with an oscillation connecting plate, the oscillation connecting plate is provided with a positioning hole, the magnetic device is provided with a positioning structure matched with the positioning hole, and when the magnetic device ascends, the positioning structure is driven to ascend and be matched with the positioning hole for positioning.
5. The oscillating magnetic force apparatus of claim 4, wherein: the positioning structure comprises a positioning pin, the lower end of the positioning pin is movably connected with the magnetic device, the upper end of the positioning pin is in positioning fit with the positioning hole, a limiting part and a spring are arranged at the upper end of the positioning pin in a sleeved mode, and the two ends of the spring respectively support against the limiting part and the magnetic device.
6. The oscillating magnetic force apparatus of claim 1, wherein: the magnetic device comprises a magnetic bar rack, and a magnetic bar group is arranged on the magnetic bar rack.
7. The oscillating magnetic force apparatus of claim 6, wherein: the magnetic bar group comprises a plurality of magnetic bars with magnetism, and the magnetic bars are distributed in a matrix manner; when the magnetic field formed by the magnetic rod acts on the sample tube of the sample holder, the upper end of the magnetic rod is positioned at one side of the lower end of the sample tube.
8. The oscillating magnetic force apparatus of claim 6, wherein: the magnetic force bar frame is provided with a lifting induction sensor and a descending induction sensor, wherein the lifting induction sensor and the descending induction sensor are arranged on the base, and the lifting induction sheet is used for triggering the lifting induction sensor and the descending induction sensor.
9. The oscillating magnetic force apparatus of claim 1, wherein: the vibration magnetic equipment further comprises an upper computer, a control card is installed on the base, and the upper computer controls starting and stopping of the vibration mechanism and the lifting mechanism through the control card.
10. A biological sample oscillation magnetic attraction method is characterized in that: installing the sample seat with the sample into the oscillation adaptive seat, and enabling the control card to control the oscillation mechanism to operate within a preset time through the upper computer, wherein the oscillation mechanism drives the oscillation adaptive seat to oscillate; when the oscillating mechanism finishes the preset oscillating time and the origin induction sheet triggers the origin induction sensor for the first time, the upper computer controls the oscillating mechanism to stop at the origin according to the feedback signal of the origin sensor; when the oscillation mechanism stops operating, the upper computer controls the lifting device to start operating, the lifting device drives the magnetic device to ascend until a magnetic field formed by the magnetic device can act on the magnetic beads in the sample seat, and the magnetic device attracts the magnetic beads in the sample seat; after the magnetism is absorbed, the lifting device drives the magnetic device to descend and keep away from the sample seat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110486158.4A CN113198360A (en) | 2021-04-30 | 2021-04-30 | Vibrating magnetic equipment and vibrating magnetic attraction method for biological sample |
Applications Claiming Priority (1)
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