CN103245611A - Multiple excitation light source system - Google Patents
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- CN103245611A CN103245611A CN2012100258620A CN201210025862A CN103245611A CN 103245611 A CN103245611 A CN 103245611A CN 2012100258620 A CN2012100258620 A CN 2012100258620A CN 201210025862 A CN201210025862 A CN 201210025862A CN 103245611 A CN103245611 A CN 103245611A
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Abstract
The invention relates to a multiple excitation light source system for providing observation and detection for a biological sample injected by a fluorescent dye. The multiple excitation light source system comprises a machine shell, a sample platform, a first light source module and a second light source module, wherein the machine shell is configured to provide an accommodation space for accommodating the light source modules and a filtering unit; the first light source module and the second light source module are light sources comprising a plurality of visible light wavelengths and/or non-visible light wavelengths; and the fluorescent dye is emitted by different light sources to make the fluorescent dye produce a relative third light wavelength source through fluorescence resonance energy superposition transfer, so as to provide the obviously observed observation and detection for the biological sample.
Description
Technical field
The present invention provides a kind of light-source system, especially for the multiple excitation light source system of the biological specimen that injects fluorescent dye being observed detection is provided.
Background technology
Along with the research of biotechnology comes into one's own gradually, detection of biological samples wherein (for example protein, cell and DNA (deoxyribonucleic acid) (DNA) etc.) also receives concern very.In the known technology, this biological specimen is to utilize fluorescence detection (fluorescence detection) to detect.This fluorescence detection is to utilize fluorescent dye to have specific excited state (excitation state) and the characteristic of radiating attitude (emission state), in order to this biological specimen is carried out mark, detect the complex molecule that comprises about this biological specimen for the result of tester by this mark and form.
Be example with this sample dna, this sample dna is to be placed on by damping fluid (for example TAE buffer) and gel (for example agar gel electrophoresis (agarose gel electrophoresis, AGE) or polyacrylamide gel electrophoresis (polyacrylamide gel electrophoresis, PAGE)) in the electrophoresis liquid of forming, and apply voltage to produce the DNA (deoxyribonucleic acid) electrophoresis, and the electrophoresis film that formation has DNA (deoxyribonucleic acid), take out again again this electrophoresis film and with this colloid inject ethidium bromide fluorescent dye (Ethidium Bromide, EtBr).Moreover the recycling ultraviolet source can be by this fluorescence to confirm the position of this DNA (deoxyribonucleic acid) on this electrophoresis film in order to the tester to be provided in order to excite fluorescent dye on this electrophoresis film to produce fluorescence.Yet this ultraviolet source is to carry out operation in specific darkroom, could observe this fluorescent dye significantly; And the light source that this ultraviolet lamp tube produces is can the effect of human body skin generation as sunshine be used the tester is long-term, is that ten minutes is unfavorable for healthy.
So in view of this present invention proposes a kind of multiple excitation light source system that can solve the shortcoming that above-mentioned known technology causes.
Summary of the invention
A purpose of the present invention provides a kind of multiple excitation light source system, and the multiple light source of forming by visible wavelength and/or non-visible light wavelength is in order to reach the effect of biological specimen being observed detection.
Another object of the present invention provides above-mentioned multiple excitation light source system, is by producing described multiple light source at diverse location, in order to strengthen the effect that manifests of this fluorescent dye on this biological specimen.
For reaching above-mentioned purpose or other purpose, the present invention is a kind of multiple excitation light source system, is be used to providing the biological specimen that injects fluorescent dye to observe detection, and it comprises casing, sample platform, first light source module and secondary light source module.This casing is to have accommodation space; This sample platform is to be arranged at this accommodation space, is for placing this biological specimen; This first light source module is a side that is arranged at this sample platform, and this first light source module is to produce first wavelength light source that is positioned at visible wavelength; And, this secondary light source module is a side that is arranged at this sample platform, this secondary light source module is to produce second wavelength light source that is positioned at visible light ripple and/or non-visible light wavelength, and this first wavelength light source and this second wavelength light source are for exciting this fluorescent dye simultaneously, and the multiple energy superposition by FRET (fluorescence resonance energy transfer) excites and produces relative three-wavelength source.
With known art, but multiple excitation light source system of the present invention provides and comprises the optical wavelength multiplex that visible wavelength and/or non-electric light wavelength are formed, the multiple energy superposition that fluorescent dye in the biological specimen is carried out FRET (fluorescence resonance energy transfer) excites, in order to produce significant wavelength of fluorescence.Moreover.In an other embodiment, the tester receives this wavelength of fluorescence by filter unit again, manifests the testing result of this biological specimen with enhancing with the filtering optical noise.In addition, according to the image difference of placing before and after this biological specimen, dynamically adjust luminance difference, white balance difference and the contrast difference of this biological specimen image again, and make the tester be convenient to carry out the observation to this biological specimen.
Description of drawings
For fully understanding purpose of the present invention, feature and effect, by following specific embodiment, and conjunction with figs., the present invention is described in detail, illustrate as after, wherein:
Fig. 1 is the diagrammatic cross-section of the multiple excitation light source system of first embodiment of the invention;
Fig. 2 is first light source module and secondary light source modules configured synoptic diagram in the key diagram 1;
Fig. 3 is the diagrammatic cross-section of the multiple excitation light source system of second embodiment of the invention;
Fig. 4 is the process synoptic diagram of fluorescent dye stimulated radiation of the multiple excitation light source system of the embodiment of the invention;
Fig. 5 is that the three-wavelength source of the multiple excitation light source system of the embodiment of the invention produces synoptic diagram;
Fig. 6 is the diagrammatic cross-section of the multiple excitation light source system of third embodiment of the invention;
Fig. 7 is the diagrammatic cross-section of the multiple excitation light source system of fourth embodiment of the invention;
Fig. 8 is the diagrammatic cross-section of the multiple excitation light source system of fifth embodiment of the invention;
Fig. 9 is the diagrammatic cross-section of the multiple excitation light source system of sixth embodiment of the invention;
Figure 10 is the diagrammatic cross-section of the multiple excitation light source system of seventh embodiment of the invention; And
Figure 11 to Figure 14 is Bioexperiment result's comparison diagram.
Embodiment
With reference to figure 1, it is the diagrammatic cross-section of the multiple excitation light source system of first embodiment of the invention.In Fig. 1, this multiple excitation light source system 100 is be used to providing the biological specimen 4 that injects fluorescent dye 2 (or can be described as fluorophor) to observe detection.Wherein, this biological specimen 4 be can be inject DNA (deoxyribonucleic acid) (deoxyribonucleic acid, DNA), the electrophoresis film of protein or biological material etc.
This multiple excitation light source system 100 is to comprise casing 12, sample platform 14, first light source module 16 and secondary light source module 18.Wherein, this casing 12 is to form lamp box, and this housing 12 have accommodation space 122 and, this sample platform 14 is to be arranged at this accommodation space 122, and is for placing this biological specimen 4 in a side of this sample platform 14.Wherein, this sample platform 14 is to can be transparence or cloudy surface shape.
This first light source module 16 is the opposite sides that are arranged at this accommodation space 122 with this secondary light source module 18, with so that this first wavelength light source FW and this second wavelength light source SW are incident to this biological specimen 4 by this sample platform 14 discriminably or side by side, as shown in Figure 2, be the configuration mode of an embodiment wherein of first light source module 16 and secondary light source module 18.
This first light source module 16 produces this first wavelength light source FW of visible wavelength.In other words, the wavelength coverage of this first wavelength light source FW is between 380 (purple light) nanometer to 750 (ruddiness) nanometer.In an embodiment, the wavelength coverage of this first wavelength light source FW is the blue light between 435 nanometers and 480 nanometers.In addition, this first light source module 16 can be made up of a plurality of luminescence unit 162, and for example described luminescence unit 162 is to be blue light diode.
This secondary light source module 18 is the second wavelength light source SW that produce visible wavelength or non-visible light wavelength.Wherein, the wavelength coverage of this second wavelength light source SW is except the scope that can comprise as aforementioned visible wavelength, also comprise the non-visible light wavelength of wavelength between 280 (extreme ultraviolet light) nanometer to 380 (black light) nanometer, more even surpass 750 (infrared lights) more than the nanometer, for example this secondary light source module 18 is to be ultraviolet lamp tube (UV), green glow and clarinet.In an embodiment, the wavelength coverage of this second wavelength light source SW is the ultraviolet light between 250 nanometers and 400 nanometers; And the wavelength coverage of this second wavelength light source SW is the green glow between 577 nanometers and 492 nanometers.Wherein, this first wavelength light source FW and this second wavelength light source SW are for exciting this fluorescent dye 2 simultaneously, and shift by fluorescence resonance superposition energy and to make this fluorescent dye produce relative three-wavelength source TW.
In other words, when this first wavelength light source FW and this second wavelength light source SW are when being incident in this fluorescent dye 2 simultaneously, this fluorescent dye 2 is to absorb first of this first wavelength light source FW simultaneously
Energy E g
1(Eg
1=hv, wherein h is Bu Langke constant 6.626 * 10
-34Joule/second; And v is light frequency) with the second energy E g of this second wavelength light source SW
2(Eg
1=hv), and (fluorescence resonance energy transfer FRET) makes this fluorescent dye 2 discharge relative the 3rd energy E g to pass through FRET (fluorescence resonance energy transfer)
3Three-wavelength source TW.
With reference to figure 3, it is the process synoptic diagram of fluorescent dye stimulated radiation of the multiple excitation light source system of second embodiment of the invention.In Fig. 3, this casing 12 that in previous embodiment, comprises, this sample platform 14, this first light source module 16 and this secondary light source module 18, more can comprise filter unit 20, it is a side that is arranged at this sample platform 14, and this filter unit 20 is to receive this three-wavelength source TW and produce relative this three-wavelength source TW three-wavelength source TW ' more clearly via the filtering behind the filtering optical noise, and for example this filter unit 20 is to be amber filter plate.In other words, the wavelength of this three-wavelength source TW ' is between the wavelength coverage of this three-wavelength source TW, only is that this three-wavelength source TW ' is the light source that comparatively is similar to single wavelength.
With reference to figure 4, it is the process synoptic diagram of fluorescent dye stimulated radiation of the multiple excitation light source system of the embodiment of the invention.In Fig. 4 (a), this fluorescent dye 2 is to absorb this first energy E g
1With this second energy E g
2, make the photon of this fluorescent dye 2 receive energy (Eg
1+ Eg
2) afterwards, transit to excited state S1 from ground state S0; Then, after number nanoseconds, this photon drops on another excited state S1 ' a little less than this excited state S1 from this excited state S1 again; And this photon is to get back to this ground state S0 again from this excited state S1 ', makes this fluorescent dye 2 discharge and has the 3rd energy E g
3Three-wavelength source TW, wherein because the minimizing of the energy of energy loss and photon, radiation wavelength always is excited wavelength greater than it, and difference between the two is Stoker displacement (Stokes shift).In other words, to discharge wavelength of fluorescence different for incident wave wavelength and fluorescent dye.In addition, each this fluorescent dye 2 all has a characteristic wavelength best, be to make this fluorescent dye 2 be excited under the wavelength (being characteristic wavelength) can seeing on the spectrum that is stimulated a certain, this fluorescent dye 2 has maximum emitting fluorescence intensity, in the lump with reference to figure 4 (b).
With reference to figure 5, be that the three-wavelength source of the multiple excitation light source system of the embodiment of the invention produces synoptic diagram.In Fig. 5, this first wavelength light source FW at visible light also comprises the first characteristic wavelength FW ', this first characteristic wavelength FW ' excites this fluorescent dye 2 in order to produce this three-wavelength source TW, for example when this fluorescent dye 2 be when selecting SYPRO RUBY for use, the wavelength coverage by this first wavelength light source FW is that the blue light between 435 nanometers and 480 nanometers excites.Wherein, this first characteristic wavelength FW ' is 470 nanometers.This fluorescent dye 2 is according to this first characteristic wavelength FW ' that absorbs, and makes this fluorescent dye 2 give off wavelength at this three-wavelength source TW of 610 nanometers.
Then, this second wavelength light source SW at non-visible light also comprises the second characteristic wavelength SW ', and this second characteristic wavelength SW ' excites this fluorescent dye 2 in order to produce as aforesaid this three-wavelength source TW, when this fluorescent dye 2 is when selecting SYPRO RUBY for use, the wavelength coverage by this second wavelength light source SW is that the ultraviolet light between 250 nanometers and 400 nanometers excites.Wherein, this second characteristic wavelength SW ' is 290 nanometers.This fluorescent dye 2 is according to this second characteristic wavelength SW ' that absorbs, and makes this fluorescent dye 2 give off wavelength at this three-wavelength source TW of 610 nanometers.
This visible light and this non-visible light are side by side to produce the have identical wavelength three-wavelength source TW of (for example 610 nanometers), and by having this first energy E g
1This first characteristic wavelength FW ' and this second energy E g
2This second characteristic wavelength SW ' superposition of carrying out energy shift, make the 3rd energy E g of this three-wavelength source TW
3Be close to or equal this first energy E g
1With this second energy E g
2The energy summation.In other words, absorbing the fluorescence intensity that this fluorescent dyes 2 of two energy produces is far above absorbing the fluorescence intensity that single energy produces.
With reference to figure 6, it is the diagrammatic cross-section of the multiple excitation light source system of third embodiment of the invention.In Fig. 6, this multiple excitation light source system 101 is to comprise this casing 12, this sample platform 14, this first light source module 16, this secondary light source module 18 and this filter unit 20 equally.Yet, different with previous embodiment is, only having only this first light source module 16 is the lower edges that are arranged at this sample platform 14, and this secondary light source module 18 is the lateral margin places that are arranged at this sample platform 14, make this second wavelength light source SW be directly oblique incidence to this biological specimen 4.In addition, in another embodiment, can't be as the light source that detects for the pointolite inequality of avoiding utilizing light emitting diode to provide in this multiple excitation light source system 102, this multiple excitation light source system 102 is that more can to comprise light diffusion unit 22 are sides that are arranged at this first light source cell 16, this the first wavelength light source FW that has area source in order to generation "; as shown in Figure 7, be the diagrammatic cross-section of the multiple excitation light source system of fourth embodiment of the invention.
With reference to figure 8, it is the diagrammatic cross-section of the multiple excitation light source system of fifth embodiment of the invention.In Fig. 8, this multiple excitation light source system 103 with the aforementioned second embodiment difference is, the position of this first light source module 16 with this secondary light source module 18 exchanged, make that this secondary light source module is the lower edge that is arranged at this sample platform 14, and this first light source module 16 is the lateral margin places that are arranged at this sample platform 14, make this first wavelength light source FW be directly oblique incidence to this biological specimen 4.
With reference to figure 9, it is the diagrammatic cross-section of the multiple excitation light source system of sixth embodiment of the invention.In Fig. 9, this first light source module 16 and this secondary light source module 18 of this multiple excitation light source system 104 is the lateral margin places that all are arranged at this sample platform 14 simultaneously, with allow this first wavelength light source FW and this second wavelength light source SW pass through this sample platform 14 directly oblique incidence to this biological specimen 4.
With reference to Figure 10, it is the diagrammatic cross-section of the multiple excitation light source system of seventh embodiment of the invention.In Figure 10, this multiple excitation light source system 105 is except being to comprise this casing 12, this sample platform 14, this first light source module 16, this secondary light source module 18 and this filter unit 20, more can comprise the upper limb place that image acquisition unit 24 is arranged on this filter unit 20, and capture corresponding image respectively in the front and back that this sample platform 14 is placed these biological specimens 4, in order to form background video BIMG and biological specimen image BSIMG.Moreover, this background video BIMG and this biological specimen image BSIMG be the comparing unit 26 by being connected with this image acquisition unit 24 again, and after the image difference between this background video and this biological specimen image relatively, detect image DIMG in order to form, be convenient to detect and observe for the tester.Wherein, this image difference is to be luminance difference, white balance difference and contrast difference.
Moreover, for can evidence multiple excitation source of the present invention be to reach that can carry out biological specimen more obviously can be for observing the effect that detects in the traditional detection.In Figure 11 to Figure 14, provide the test result of a plurality of experiments, to verify that multiple excitation light source system of the present invention is to injecting the effect that biological specimen was produced of fluorescent dye (or can be described as fluorophor).In this, this biological specimen is to be the example explanation with protein electrophorese glue.
Please refer to Figure 11, in the first experiment contrast group, provide protein electrophorese film (SDS-PAGE).Wherein, experimental conditions is to form with three road steps respectively, is that to be respectively first road be standard protein molecular weight marker thing 10; L, second road is 5 μ l, the 3rd road is 2.5 μ l, follow-up decrement successively half, moreover in this group experiment contrast group, this protein electrophorese film is to use SYPRO Ruby dyeing.This first experiment contrast picture group 11 (a) is the state that shows after this protein electrophorese film is via single UV-irradiation; This first experiment contrast picture group 11 (b) is the state that shows after this protein electrophorese film is via single blue light illumination; And this first experiment contrast picture group 11 (c) is to show that this protein electrophorese film is via comprising the state that blue light and ultraviolet light carry out multiple excitation source irradiation simultaneously.Via more above-mentioned three kinds of states, be to find obviously that it is to send obviously to observe the optical wavelength that detects for row with Figure 11 (b) that this first experiment contrast picture group 11 (c) is compared Figure 11 (a).
Please refer to Figure 12, in the second experiment contrast group, provide DNA agar electrophoresis.Wherein, experimental conditions is to form with three road steps respectively, is that to be respectively first road be standard DNA molecular weight marker thing 500ng, and second road is 250ng, and the 3rd road is 125ng, follow-up decrement successively half.Moreover in this group experiment contrast group, this DNA agar electrophoresis is to use SYBR Green I dyeing.This second experiment contrast picture group 12 (a) is the state that shows after this DNA agar electrophoresis is via single UV-irradiation; This second experiment contrast picture group 12 (b) is the state that shows after this DNA agar electrophoresis is via single blue light illumination; And this second experiment contrast picture group 12 (c) is to show that this DNA agar electrophoresis is via comprising the state that blue light and ultraviolet light carry out multiple excitation source irradiation simultaneously.Via more above-mentioned three kinds of states, be to find obviously that it is to send obviously to supply to observe the optical wavelength that detects with Figure 12 (b) that this second experiment contrast picture group 12 (c) is compared Figure 12 (a).
Please refer to Figure 13, in the 3rd experiment contrast group, provide 100ug BSA protein and directly add SYPRO Ruby and be positioned in the little transparent pipe.Wherein, the 3rd experiment contrast picture group 13 (a) is to be presented at this BSA protein in this little transparent pipe state after via single UV-irradiation; The 3rd experiment contrast picture group 13 (b) is to be presented at this BSA protein in this little transparent pipe state after via single blue light illumination; And the 3rd experiment contrast picture group 13 (c) is that this BSA protein that is presented in this little transparent pipe carries out the state that multiple excitation source shines simultaneously via comprising blue light and ultraviolet light.Via more above-mentioned three kinds of states, be to find obviously that it is to send obviously to supply to observe the optical wavelength that detects with Figure 13 (b) that the 3rd experiment contrast picture group 13 (c) is compared Figure 13 (a).
Please refer to Figure 14, in the 4th experiment contrast group, provide 10 μ g DNA protein and directly add SYBR Green I and be positioned in the little transparent pipe.Wherein, the 4th experiment contrast picture group 14 (a) is to be presented at this DNA protein in this little transparent pipe state after via single blue light illumination; The 4th experiment contrast picture group 14 (b) is to be presented at this DNA protein in this little transparent pipe via the postradiation state of single green glow; And the 4th experiment contrast picture group 14 (c) is that this DNA protein that is presented in this little transparent pipe carries out the state that multiple excitation source shines simultaneously via comprising blue light and green glow.Via more above-mentioned three kinds of states, be to find obviously that it is to send obviously to supply to observe the optical wavelength that detects with Figure 14 (b) that the 4th experiment contrast picture group 14 (c) is compared Figure 14 (a).
So but above-described embodiment is to provide the optical wavelength multiplex that visible wavelength and non-electric light wavelength are formed for explanation multiple excitation light source system of the present invention, the multiple energy superposition that fluorescent dye in the biological specimen is carried out FRET (fluorescence resonance energy transfer) excites, in order to produce significant wavelength of fluorescence.In addition, the tester receives this wavelength of fluorescence by filter unit again, manifests the testing result of this biological specimen with enhancing with the filtering optical noise.In addition, the tester according to the image difference of placing before and after this biological specimen, dynamically adjusts luminance difference, white balance difference and the contrast difference of this biological specimen image again, and makes the tester be convenient to carry out the observation to this biological specimen.In addition, according to the image difference of placing before and after this biological specimen, dynamically adjust luminance difference, white balance difference and the contrast difference of this biological specimen image again, and make the tester be convenient to carry out the observation to this biological specimen.
The present invention discloses with preferred embodiment hereinbefore, so has the knack of this operator and it should be understood that this embodiment only is used for describing the present invention, does not limit the scope of the invention and should not be read as.It should be noted that variation and the displacement of every and this embodiment equivalence all should be made as and be covered by in the category of the present invention.Therefore, protection scope of the present invention is worked as with being as the criterion that the claim scope is defined.
Claims (22)
1. a multiple excitation light source system is used for providing the biological specimen that injects fluorescent dye to observe detection, and it comprises:
Casing has accommodation space;
The sample platform is arranged at this accommodation space, for placing this biological specimen;
First light source module is arranged at a side of this sample platform, and this first light source module produces first wavelength light source that is positioned at visible wavelength; And
The secondary light source module, be arranged at a side of this sample platform, this secondary light source module produces the one person's who is positioned at visible wavelength and non-visible light wavelength second wavelength light source, and this first wavelength light source and this second wavelength light source are used for exciting simultaneously this fluorescent dye, and the multiple energy superposition by FRET (fluorescence resonance energy transfer) excites and makes this fluorescent dye produce relative three-wavelength source.
2. multiple excitation light source system as claimed in claim 1 also comprises the side that filter unit is arranged at this sample platform, and this filter unit receives this three-wavelength source and via filter unit filtering optical noise.
3. multiple excitation light source system as claimed in claim 1, wherein this first light source module has a plurality of luminescence units.
4. multiple excitation light source system as claimed in claim 3, wherein said luminescence unit is light emitting diode.
5. multiple excitation light source system as claimed in claim 4 also comprises the light diffusion unit, is arranged at a side of this first light source cell, has this first wavelength light source of area source in order to generation.
6. multiple excitation light source system as claimed in claim 3, wherein this first wavelength light source also comprises first characteristic wavelength, and this first characteristic wavelength excites this fluorescent dye to produce this three-wavelength source.
7. multiple excitation light source system as claimed in claim 6, the wherein blue light of the wavelength coverage of this first wavelength light source between 435 nanometers and 480 nanometers.
8. multiple excitation light source system as claimed in claim 7 when wherein this first characteristic wavelength is 470 nanometers, produces wavelength in this three-wavelength source of 610 nanometers in order to excite this fluorescent dye.
9. multiple excitation light source system as claimed in claim 1, wherein this second wavelength light source also comprises second characteristic wavelength, and this second characteristic wavelength excites this fluorescent dye to produce this three-wavelength source.
10. multiple excitation light source system as claimed in claim 7, the wherein ultraviolet light of the wavelength coverage of this second wavelength light source between 250 nanometers and 400 nanometers.
11. multiple excitation light source system as claimed in claim 10 when wherein this second characteristic wavelength is 290 nanometers, produces wavelength in this three-wavelength source of 610 nanometers in order to excite this fluorescent dye.
12. multiple excitation light source system as claimed in claim 11, wherein this secondary light source module is ultraviolet lamp tube, green glow or clarinet.
13. multiple excitation light source system as claimed in claim 1, wherein this sample platform is the person of one at least of transparence and cloudy surface shape.
14. multiple excitation light source system as claimed in claim 13, wherein this first light source module and this secondary light source module are arranged at the lower edge of this sample platform, are incident to this biological specimen for this first wavelength light source and this second wavelength light source by this sample platform.
15. multiple excitation light source system as claimed in claim 13, wherein the one person of this first light source module or this secondary light source module is arranged at the lower edge of this sample platform, and other one person is arranged at the lateral margin place of this sample platform.
16. multiple excitation light source system as claimed in claim 15, wherein this first wavelength light source or this second wavelength light source directly oblique incidence to this biological specimen.
17. multiple excitation light source system as claimed in claim 13, wherein this first light source module and this secondary light source module are arranged at the lateral margin place of this sample platform, for this first wavelength light source and this second wavelength light source by this sample platform directly oblique incidence to this biological specimen.
18. multiple excitation light source system as claimed in claim 1, also comprise image acquisition unit, be arranged on the upper limb place of this filter unit, and capture corresponding image respectively in the front and back of this this biological specimen of sample platform placement, in order to form background video and biological specimen image.
19. multiple excitation light source system as claimed in claim 18 also comprises comparing unit, is connected to this image acquisition unit in order to the image difference between this background video and this biological specimen image relatively, detects image to form.
20. multiple excitation light source system as claimed in claim 19, wherein this image difference is luminance difference, self-equilibrating difference and contrast difference.
21. multiple excitation light source system as claimed in claim 1, wherein this filter unit is amber filter plate.
22. multiple excitation light source system as claimed in claim 1, wherein this biological specimen is the electrophoresis film.
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Application publication date: 20130814 |