CN213447070U

CN213447070U - Internal circulation device for extracting large-flux nucleic acid

Info

Publication number: CN213447070U
Application number: CN202021412858.6U
Authority: CN
Inventors: 黄挺; 张恒; 张博; 张晓婷
Original assignee: Xi'an Biolab Biotechnology Co ltd
Current assignee: Xi'an Biolab Biotechnology Co ltd
Priority date: 2020-07-16
Filing date: 2020-07-16
Publication date: 2021-06-15
Anticipated expiration: 2030-07-16

Abstract

The utility model discloses an internal circulating device is used in extraction of large flux nucleic acid, internal circulating device at least include the wind channel, wind channel laminating nucleic acid extraction equipment internal surface one end sets up, along inside air intake, circulation power component, filtering component and the air outlet of having set gradually in the wind channel. It can filter the inside air of purifying instrument in time of this device, prevents that the aerosol from spilling over, forms cross infection, influences experiment testing result. Meanwhile, the air inside the instrument is isolated from the outside air in the experiment operation process of the experimenters, and the biological risk and subsequent detection interference factors of the experimenters are eliminated.

Description

Internal circulation device for extracting large-flux nucleic acid

Technical Field

The utility model relates to a nucleic acid extraction technical field, in particular to internal circulation device is used in extraction of large flux nucleic acid.

Background

With the rapid development of molecular biology techniques in recent years, molecular diagnosis and detection techniques typified by nucleic acid hybridization, nucleic acid amplification, and nucleic acid sequence analysis have increasingly highlighted important roles in various fields. Novel molecular biological detection techniques include Polymerase Chain Reaction (PCR), microchips, high-throughput sequencing, and the like. However, all modern molecular biology detection techniques must rapidly and effectively isolate and extract the desired genomic nucleic acid from complex and diverse biological samples, and the quality and integrity of the extracted nucleic acid directly affect the results of subsequent experiments.

At present, a large-flux nucleic acid extraction device generally adopts a nano magnetic bead method to separate and extract biomolecules, and the method needs a magnetic rod, a stirring sleeve and corresponding reagents. However, because the equipment flux is large and the number of samples is large, the whole process lifts the nano magnetic beads adsorbed with target biomolecules away from the liquid surface through the magnetic rod and the stirring sleeve for many times to expose the nano magnetic beads in the air, so that the biomolecules are easy to form aerosol in the air, biological risks exist for experimenters, and the subsequent detection can be interfered.

Disclosure of Invention

The utility model aims at providing a big flux nucleic acid draws uses internal circulating device has solved the problem that exists among the prior art.

In order to realize the technical task, the utility model discloses a following technical scheme realizes:

the utility model provides an internal circulating device is used in extraction of large flux nucleic acid, internal circulating device include the wind channel at least, wind channel laminating nucleic acid extraction equipment internal surface one end sets up, has set gradually air intake, circulation power subassembly, filtering component and air outlet along wind channel inside.

Furthermore, a disinfection module is arranged on one side of the air outlet of the air duct.

Further, the filter assembly is a combination of a filler type filter assembly and a high-efficiency filter assembly.

Preferably, the filler-type filter assembly is an activated carbon filter assembly.

Preferably, the high-efficiency filtering component is a HEPA filter. The disinfection module is an ultraviolet disinfection lamp.

Furthermore, the air inlet is provided with one or more air inlets at intervals along one end of the air duct, and the air inlets and the air outlets are arranged on two sides of the air duct at intervals.

Compared with the prior art, the utility model following profitable technological effect has:

adopt the nucleic acid of this application to draw inside circulating device of instrument, instrument inside air filtration circulation system promptly, it can in time filter the inside air of purification instrument, prevents that the aerosol from spilling over, forms cross infection, influences experiment testing result. Meanwhile, the air inside the instrument is isolated from the outside air in the experiment operation process of the experimenters, and the biological risk and subsequent detection interference factors of the experimenters are eliminated.

Drawings

Fig. 1 is a cross-sectional view of the overall structure of the utility model.

The following are the meanings indicated in the figures, respectively, by reference numerals:

1, an air outlet; 2, a disinfection module; 3, an active carbon filter component; 4, a high-efficiency filtering component; 5, circulating a power assembly; 6, 8 air inlets; 7, an air duct; 9, a nucleic acid extraction instrument shell; 10, a reaction chamber; 11, a sterilization module;

the following detailed description of the present invention will be made with reference to the accompanying drawings and the detailed description.

Detailed Description

Referring to fig. 1, the utility model discloses an internal circulating device is used in extraction of large flux nucleic acid, internal circulating device include wind channel 7 at least, the setting of wind channel 7 laminating nucleic acid extraction equipment internal surface one end has set gradually air intake (6, 8), circulation power component 5, filtering component and air outlet 1 along wind channel 7 inside.

Further, a disinfection module 2 is arranged on one side of the air outlet 1 of the air duct 7.

In order to improve the inside aerosol adsorption filtration effect of nucleotide extraction equipment better, the utility model discloses can set filter assembly to be filler class filter assembly and high-efficient filter assembly's combination.

Preferably, the filler-based filter assembly is an activated carbon filter assembly. The high-efficiency filtering component is a HEPA filter. The disinfection module 2 is an ultraviolet disinfection lamp.

In order to better improve the ventilation circulation effect of the whole large-flux nucleic acid extraction equipment, the air inlets related to the application can be arranged at intervals along one end of the air duct 7 to be one or more, namely the air inlets (6, 8) are shown in the figure, so that the air inlets (6, 8) and the air outlet 1 are arranged at two sides of the air duct 7 at intervals.

The following is the concrete operation process of the internal circulation device for extracting the large-flux nucleic acid of the utility model:

firstly, the power supply of the instrument is turned on, the internal circulation power component 5 is automatically started, so that air in the reaction chamber 10 flows into the air channel 7 from the air inlets 6 and 8, passes through the high-efficiency filtering component 4 and the active carbon filtering component 3, and then flows into the reaction chamber 10 from the air outlet 1, and the internal air circulation of the instrument is completed.

And secondly, continuously and repeatedly circulating in the experiment process, and taking out the kit after the experiment is finished.

Thirdly, manually starting the

ultraviolet disinfection modules

2 and 11 to start disinfection, wherein the disinfection time can be set automatically;

and fourthly, after the disinfection is finished, the power supply of the instrument is turned off, the internal circulation power assembly 4 is turned off, and the circulation is finished.

The disinfection modules (2,11) that this application relates to are ultraviolet disinfection lamp, and in whole experimentation automatic shutdown, manual opening after the experiment finishes. Particularly, the ultraviolet disinfection lamp can inactivate all bacteria and viruses and deteriorate protein, so that after the experiment is finished, the kit must be taken out firstly, and then the ultraviolet disinfection module is manually started for disinfection;

the application provides an internal circulation device is used in extraction of large flux nucleic acid can ensure that the nucleic acid draws the inside air of instrument constantly to purify the circulation in the experimentation, prevents aerosol cross infection, influences the experiment. Simultaneously, make the whole encapsulated situation that is in of experiment in-process instrument, with instrument inside air and outside air isolated, eliminate experimenter's by biological erosion risk. The device related to the application has been successfully applied to the experimental operation process of reality, and the effect is obvious.

Claims

1. An internal circulation device for large-flux nucleic acid extraction is characterized in that: the internal circulation device at least comprises an air duct (7), the air duct (7) is attached to one end of the inner surface of the nucleic acid extraction equipment, and air inlets (6 and 8), a circulation power assembly (5), a filtering assembly and an air outlet (1) are sequentially arranged along the inside of the air duct (7).

2. The internal circulation device for large-throughput nucleic acid extraction according to claim 1, wherein: and a disinfection module (2,11) is also arranged in parallel at one side of the air outlet (1) of the air duct (7).

3. The internal circulation device for large-throughput nucleic acid extraction according to claim 1 or 2, wherein: the filter assembly is a combination of a filler type filter assembly and a high-efficiency filter assembly.

4. The internal circulation device for large-throughput nucleic acid extraction according to claim 3, wherein: the filler filtering component is an activated carbon filtering component.

5. The internal circulation device for large-throughput nucleic acid extraction according to claim 3, wherein: the high-efficiency filtering component is a HEPA filter.

6. The internal circulation device for large-throughput nucleic acid extraction according to claim 2, wherein: the disinfection modules (2,11) are ultraviolet disinfection lamps.

7. The internal circulation device for large-throughput nucleic acid extraction according to claim 3, wherein: one or more air inlets (6, 8) are arranged at intervals along one end of the air duct (7), and the air inlets (6, 8) and the air outlet (1) are arranged at two sides of the air duct (7) at intervals.