CN215103229U - Nucleic acid extraction and PCR detection all-in-one machine - Google Patents
Nucleic acid extraction and PCR detection all-in-one machine Download PDFInfo
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- CN215103229U CN215103229U CN202121290962.7U CN202121290962U CN215103229U CN 215103229 U CN215103229 U CN 215103229U CN 202121290962 U CN202121290962 U CN 202121290962U CN 215103229 U CN215103229 U CN 215103229U
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 55
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 55
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 55
- 238000000605 extraction Methods 0.000 title claims abstract description 49
- 238000001514 detection method Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 238000001914 filtration Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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Abstract
The utility model relates to the technical field of nucleic acid detection, in particular to an integrated machine for nucleic acid extraction and PCR detection, which comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the machine shell covers the whole equipment; an air inlet is formed above the shell, and an air outlet is formed in the right side of the shell; a first filter is arranged at the air inlet; the bottom of the nucleic acid extraction module is provided with a first air duct, the bottom of the PCR detection module is provided with a second air duct, and the first air duct is communicated with the second air duct; the rear side of the second air duct is sequentially provided with a turning air duct and a fan, a second filter is arranged on the side face of the PCR detection module, the fan is communicated with the second filter, and the second filter is communicated with the air outlet; the utility model discloses in time filter volatile reagent and arrange to the air in, avoid the polluted environment, ensured the accuracy that PCR detected.
Description
Technical Field
The utility model relates to a nucleic acid detects technical field, especially relates to a nucleic acid draws and PCR detects all-in-one.
Background
Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction for separation and purification of biomolecules. The nucleic acid extractor is an instrument which automatically finishes nucleic acid extraction work by using a matched nucleic acid extraction reagent, and is widely applied to the fields of clinical disease diagnosis, microbiological detection and the like; after nucleic acid extraction, PCR detection, which is a molecular biological technique for amplifying specific DNA fragments, is required. The nucleic acid extraction and PCR detection integrated machine applied to the market at present has the advantages that the nucleic acid extraction module and the PCR detection module are not isolated, the nucleic acid extract is polluted in the using process of equipment, and the quality of the surrounding environment is influenced.
Chinese patent CN212051333U discloses a full-automatic nucleic acid extraction and fluorescence PCR instrument integrated machine, which comprises a nucleic acid extractor main body, a base, an extraction component, a detection module, a sample container top cover, a sample container, an extraction support frame, a temperature raising and reducing module, an extraction basket movement component, a liquid-transferring and sample-adding module, a driving module and a guide rail, wherein the extraction component is fixed on the outer wall of the middle part between two sides of the nucleic acid extractor main body, the base is arranged on the outer wall of the bottom end of the nucleic acid extractor main body, the extractor adopts the combination of the nucleic acid extractor and the fluorescence PCR instrument, so that after nucleic acid is extracted, DNA amplification is carried out by a stand horse, the working efficiency is improved, and an automatic liquid-transferring and sample-adding module and an extraction basket movement device are adopted, so that the sample is added into the reaction container for reaction, the sample and the base are moved relatively, the movement distance of the liquid-transferring and sample-adding module is shortened, the service life of the liquid-transferring and sample-adding module can be prolonged, the complexity of the instrument is reduced, and the reliability of the instrument is improved.
In the prior art, in the working process of the integrated nucleic acid extraction and PCR detection machine, because liquid reagents are used during nucleic acid extraction, the liquid reagents are easy to volatilize, the volatilized reagents leak into the atmosphere to further influence the surrounding air environment, and gaseous reagents left in the equipment can pollute the reagents for extracting nucleic acid at the next time; if the nucleic acid extraction module and the PCR detection module are not isolated, the leaked reagents can also affect the accuracy of the PCR detection.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model provides a nucleic acid extraction and PCR detection all-in-one machine.
The technical scheme of the utility model as follows:
a nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the machine shell covers the whole equipment, the nucleic acid extraction module is positioned on the left side in the machine shell, and the PCR detection module is positioned on the right side of the machine shell; a lifting door is arranged between the nucleic acid extraction module and the PCR detection module; an air inlet is formed above the shell, and an air outlet is formed in the right side of the shell; a first filter is arranged at the air inlet; a first air duct is arranged at the bottom of the nucleic acid extraction module, a second air duct is arranged at the bottom of the PCR detection module, and the first air duct is communicated with the second air duct; the rear side of the second air channel is sequentially provided with a turning air channel and a fan, a second filter is arranged on the side face of the PCR detection module, the fan is communicated with the second filter, and the second filter is communicated with the air outlet.
Furthermore, the air inlets are a plurality of round holes arranged in an array.
Furthermore, an air inlet fan is arranged below the first filter, so that air circulation is increased, and the ventilation effect is improved.
Furthermore, an air receiving opening is formed between the first air duct and the second air duct, so that the air flow in the first air duct can be gathered into the second air duct.
Further, the lifting door is installed on the PCR detection module.
The utility model has the advantages that:
1. the nucleic acid extraction module and the PCR detection module are isolated by the lifting door, and the accuracy of PCR detection cannot be influenced by leaked reagents during nucleic acid extraction;
2. the utility model is provided with the special air channel with high-efficiency filtering function, when the air inlet fan and the fan at the rear side of the equipment start to work, the air flow enters from the air inlet, goes downwards along the left side of the nucleic acid extraction module and enters into the first air channel, then the leaked reagent enters into the second air channel along with the air flow along the air receiving port, then the air flow sequentially passes through the diversion air channel and the fan, enters into the filter screen for filtering, and the filtered air flow is discharged into the air from the air outlet; leaked reagents are quickly filtered, the working environment of equipment is purified, and the next nucleic acid extraction is facilitated; and simultaneously, the pollution of the surrounding atmospheric environment is avoided.
Drawings
FIG. 1 is a schematic structural diagram of the nucleic acid extraction and PCR detection integrated machine provided by the present invention.
FIG. 2 is a schematic view of an air duct of the nucleic acid extraction and PCR detection integrated machine shown in FIG. 1
In the figure, 1, a machine shell, 2, a nucleic acid extraction module, 3, a PCR detection module, 4, a lifting door, 5, an air inlet, 6, an air outlet, 7, a first filter, 8, a first air channel, 9, a second air channel, 10, a direction-changing air channel, 11, a fan, 12, a second filter, 13 and an air receiving port.
Detailed Description
The technical solution of the present invention will be described clearly with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that the terms "center", "upper", "lower", "horizontal", "left", "right", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.
As shown in fig. 1-2, the utility model provides an integrated machine for nucleic acid extraction and PCR detection, comprising a casing 1, a nucleic acid extraction module 2 and a PCR detection module 3, wherein the casing 1 covers the whole device, the nucleic acid extraction module 2 is located at the left side inside the casing 1, and the PCR detection module 3 is located at the right side inside the casing 1; a lifting door 4 is arranged between the nucleic acid extraction module 2 and the PCR detection module 3, the lifting door 4 is installed on the PCR detection module 3, and the lifting door 4 and other components of the PCR detection module 3 together seal the PCR detection module 3 into an independent chamber; an air inlet 5 is arranged above the machine shell 1, and an air outlet 6 is arranged on the right side of the machine shell 1; a first filter 7 is arranged at the position of the air inlet 5; a first air duct 8 is arranged at the bottom of the nucleic acid extraction module 2, a second air duct 9 is arranged at the bottom of the PCR detection module 3, and the first air duct 8 is communicated with the second air duct 9; the rear side of the second air duct 9 is sequentially provided with a turning air duct 10 and a fan 11, the side surface of the PCR detection module 3 is provided with a second filter 12, the fan 11 is communicated with the second filter 12, and the second filter 12 is communicated with the air outlet 6.
Further, the air inlet 5 is a plurality of round holes arranged in an array.
Furthermore, an air inlet fan is arranged below the first filter 7, so that air circulation is increased, and the ventilation effect is increased.
Further, an air receiving opening 13 is arranged between the first air duct 8 and the second air duct 9, so that the air flow of the first air duct 8 can be gathered in the second air duct 9.
As shown by the arrow in fig. 1, when the air intake fan and the fan 11 at the rear side of the device start to work, the air flow enters from the air inlet 5, goes downward along the left side of the nucleic acid extraction module 2, enters the first air channel 8, then enters the second air channel 9 along the air receiving port 13, then sequentially passes through the diversion air channel 10 and the fan 11, enters the filter screen for filtering, and then is discharged into the air from the air outlet 6; the utility model discloses can filter pollutants such as dust, pathogen in the air current, filtration efficiency reaches more than 99.9%.
The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit, although the present invention has been described in detail with reference to the examples, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the scope of the technical solutions of the present invention, and all of them should be covered in the scope of the claims of the present invention.
Claims (5)
1. A nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the machine shell covers the whole equipment, the nucleic acid extraction module is positioned on the left side in the machine shell, and the PCR detection module is positioned on the right side of the machine shell; the method is characterized in that: a lifting door is arranged between the nucleic acid extraction module and the PCR detection module; an air inlet is formed above the shell, and an air outlet is formed in the right side of the shell; a first filter is arranged at the air inlet; a first air duct is arranged at the bottom of the nucleic acid extraction module, a second air duct is arranged at the bottom of the PCR detection module, and the first air duct is communicated with the second air duct; the rear side of the second air channel is sequentially provided with a turning air channel and a fan, a second filter is arranged on the side face of the PCR detection module, the fan is communicated with the second filter, and the second filter is communicated with the air outlet.
2. The integrated machine for nucleic acid extraction and PCR detection as claimed in claim 1, wherein: the air inlet is a plurality of round holes arranged in an array.
3. The integrated machine for nucleic acid extraction and PCR detection as claimed in claim 1, wherein: an air inlet fan is arranged below the first filter.
4. The integrated machine for nucleic acid extraction and PCR detection as claimed in claim 1, wherein: an air receiving opening is arranged between the first air duct and the second air duct.
5. The integrated machine for nucleic acid extraction and PCR detection as claimed in claim 1, wherein: the lifting door is arranged on the PCR detection module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121290962.7U CN215103229U (en) | 2021-06-09 | 2021-06-09 | Nucleic acid extraction and PCR detection all-in-one machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121290962.7U CN215103229U (en) | 2021-06-09 | 2021-06-09 | Nucleic acid extraction and PCR detection all-in-one machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215103229U true CN215103229U (en) | 2021-12-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121290962.7U Active CN215103229U (en) | 2021-06-09 | 2021-06-09 | Nucleic acid extraction and PCR detection all-in-one machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215103229U (en) |
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2021
- 2021-06-09 CN CN202121290962.7U patent/CN215103229U/en active Active
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