CN209210832U - A kind of portable augmentation detection instrument - Google Patents

Abstract

The utility model provides a kind of portable augmentation detection instrument, is related to testing equipment field, including cabinet, detection cabin, battery, control module and fluorescence detection module；It detects and is equipped with heating module in cabin, heating module is electrically connected with battery；Fluorescence detection module includes the image storage apparatus for glimmering light activated optical module and for recording fluorescent reaction；Optical module includes the light emitting diode set gradually, lens, optical filter and dichroscope, and light emitting diode is electrically connected with battery；Control module includes the mobile phone of logic control element and control logic control unit, and battery, heating module, light emitting diode are electrically connected with logic control element and are controlled by logic control element；The logic control element is set as single-chip microcontroller.The beneficial effects of the utility model are: the integrated of amplified reaction and fluorescence detection may be implemented, it is convenient for carrying simultaneously.

Description

A kind of portable augmentation detection instrument

Technical field

The utility model relates to testing equipment field, in particular to a kind of portable augmentation detection instrument.

Background technique

Detection of nucleic acids based on microflow control technique can be by 3 steps --- the nucleic acid extraction, nucleic acid of traditional detection of nucleic acids Amplification, amplified production detection --- be integrated on a platform, can be very good to solve traditional nucleic acid detection method it is cumbersome, The numerous and jumbled disadvantage of instrument, while the advantage for having and saving reagent, save the time, automate prospect.

Although current existing micro-fluidic nucleic acid detection system possesses good integrated level and flux properties, but because designing module Numerous, detection system is still bulky, testing cost is expensive, it is difficult to realize real-time test, especially augmentation detection instrument, process It is at high cost, it cannot achieve miniaturization, can not carry.

Utility model content

In order to solve the above-mentioned technical problem, a kind of portable augmentation detection instrument is disclosed in the utility model, this is practical new The technical solution of type is implemented:

A kind of portable augmentation detection instrument, including cabinet and setting the intracorporal detection cabin of case, battery, control module and Fluorescence detection module；Heating module is equipped in the detection cabin, the heating module is electrically connected with the battery；The fluorescence inspection Surveying module includes the image storage apparatus for glimmering light activated optical module and for recording fluorescent reaction；The light Learning module includes the light emitting diode set gradually, optical filter, dichroscope and lens, the light emitting diode and the battery Electrical connection；The control module includes the mobile phone of logic control element and control logic control unit, the battery, it is described plus Thermal modules, the light emitting diode are electrically connected with the logic control element and are controlled by the logic control element；It is described Logic control element is set as single-chip microcontroller.

Preferably, the heating module includes heating copper post, and the heating copper post is set as internal several resistance wires of setting Copper post.

Preferably, the heating module further includes the heating heat lid of covering heating copper post, and the heating heat lid is by ITO glass Glass is made.

Preferably, radiator is equipped in the cabinet, the radiator is set as several towards the light emitting diode Cooling fin.

Preferably, described image storage equipment is the camera of the mobile phone.

Preferably, the optical module is arranged above heating module, and described image stores equipment and is arranged in the optics Above module.

Implement the beneficial effects of the utility model are:

1, augmentation detection instrument can provide stable amplification environment, and be excited by ultraviolet light to sample, utilize mobile phone Amplification is detected, realizes the integrated of amplified reaction and fluorescence detection, battery is arranged in augmentation detection instrument, can get rid of reality Room environmental is tested, is convenient for carrying.

2, sample amplification may be implemented by the control of mobile phone and fluorescence detection is integrated together, and be automatically finished, wrap The setting of amplification program and the presentation of amplification are included, human-computer interaction may be implemented.

Detailed description of the invention

In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is an embodiment of the present invention, for those of ordinary skill in the art, in the premise not made the creative labor Under, it is also possible to obtain other drawings based on these drawings.

Fig. 1 is the structural schematic diagram of specific embodiment；

Fig. 2 is in specific embodiment for embodying the structural schematic diagram of control module；

Fig. 3 is in specific embodiment for embodying the structural schematic diagram of optical module；

Fig. 4 is in specific embodiment for embodying the structural schematic diagram of mobile phone and cabinet connection type；

Fig. 5 is in specific embodiment for embodying the structural schematic diagram of heating heat lid

In above-mentioned attached drawing, each figure number label is respectively indicated:

1- cabinet；11- detects cabin；12- battery；13- logic control element；2- optical module；21- light emitting diode；22- Lens；23- optical filter；24- dichroscope；3- mobile phone；5- heating heat lid；51- heats copper post；6- radiator.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts Every other embodiment obtained, fall within the protection scope of the utility model.

Wherein identical components are presented with like reference characters.It should be noted that word used in the following description Language "front", "rear", "left", "right", "up" and "down" refer to the direction in attached drawing, word " bottom surface " and " top surface ", "inner" and "outside" refers respectively to the direction towards or away from geometric center of specific component.

In a specific embodiment, a kind of portable augmentation detection instrument, as shown in figures 1 and 3, including cabinet 1 With detection cabin 11, battery 12, control module 13 and the fluorescence detection module being arranged in cabinet 1；It detects to be equipped in cabin 11 and add Thermal modules, heating module are electrically connected with battery 12；Fluorescence detection module includes for glimmering light activated optical module 2 and being used for Record the image storage apparatus of fluorescent reaction；Optical module 2 include set gradually light emitting diode 21, optical filter 23, Dichroscope 24 and lens 22, light emitting diode 21 are electrically connected with battery 12；Control module include logic control element 13 and The mobile phone 3 of control logic control unit 13, battery 12, heating module, light emitting diode 21 and logic control element 13 are electrically connected It connects and is controlled by logic control element 13.

As depicted in figs. 1 and 2, by the setting of heating module, sample can be provided and increase the reaction condition for expanding reaction, made With in the process, sample to be amplified is placed in the heating module in test chamber 11 first, heating module is carried out by mobile phone 3 Parameter setting, and heating module work is controlled by logic control element 13, heating module provides for the amplified reaction of sample Certain temperature environment completes the constant-temperature amplification of sample.

As shown in Figures 2 and 3, after sample completes amplified reaction, control light emitting diode 21 works, Light-Emitting Diode 21 Luminous energy is converted electrical energy into, the light that light emitting diode 21 gives out is filtered by optical filter 23, is realized by dichroscope 24 Burst of ultraviolel focuses on image storage apparatus finally by lens 22, and being generated fluorescence performance by burst of ultraviolel on sample can be with It is recorded by image storage apparatus, it is yellowish green to release calcium in conjunction with manganese ion for the pyrophosphate ion generated during sample amplification Element, therefore the varying strength shown by fluorescence can reflect the degree of amplification, realize and examine to the fluorescence after sample amplification reaction Survey, the integrated of amplified reaction device and detection device may be implemented, can also during sample completes amplified reaction into Row detection, and then the case where can be observed in real time during example reaction.

As depicted in figs. 1 and 2, augmentation detection instrument can provide stable amplification environment to sample, and by ultraviolet light to sample Product are excited, and are finally detected using mobile phone 3 to amplification, battery 12 is built in augmentation detection instrument, heating module The electric energy to work with light emitting diode 21 is provided by the battery 12 being arranged in cabinet 1, and battery 12 is convenient for carrying and facilitates more It changes, laboratory environment can be got rid of, expanded in field, for realizing that the real-time test of sample has great importance.One In a preferred embodiment, as shown in figure 4, image storage apparatus is the camera of above-mentioned mobile phone 3, mobile phone 3 is taken the photograph when use As head alignment detection cabin, directly use the camera of mobile phone 3 as image storage apparatus and record the fluorescence reaction of sample, conveniently It is laborsaving, it is possible to reduce external equipment is hindered caused by carrying, 3 one side control logic control unit 13 of mobile phone, on the other hand The fluorescence reaction for recording sample, can also reduce the additional operations of extraneous instrument, streamline operation also can be used existing Small image storage equipment is external, can be replaced according to actually detected situation, cabinet 1 is dimensioned in present embodiment 15x8x6cm3 is close with mobile phone size.

As shown in Fig. 2, human-computer interaction may be implemented by 3 control logic control unit 13 of mobile phone, including amplification program The presentation of setting and amplification, mobile phone 3 can be connect by wireless modes such as bluetooths with logic control element 13, can also be led to The connection of the wired modes such as USB interface is crossed, sample amplification may be implemented by the control of mobile phone 3 and fluorescence detection is integrated together, And be automatically finished, realize the detection function being just able to achieve by huge detecting instrument in the prior art, solve due to Augmentation detection instrument it is larger or it is more accurate be difficult to the problem of carrying, increase augmentation detection instrument portability, sample may be implemented Real-time detection.

In one preferred embodiment, as shown in Fig. 2, logic control element 13 is set as single-chip microcontroller, single-chip microcontroller is allusion quotation The embedded microcontroller (Microcontroller Unit) of type, by arithmetic unit, controller, memory, input-output equipment Deng composition, it is equivalent to a miniature computer, compared with applying the universal microprocessor in PC, it is more emphasized From supply (do not have to external hardware) and save the cost, its great advantage be it is small in size, can be set in cabinet 1 (with reference to figure 1) internal, and then keep augmentation detection instrument volume smaller, more portable, other can be used for being arranged in cabinet 1 in the prior art Small logic control unit 13 is also suitable, then this does not illustrate in detail.

In one preferred embodiment, as shown in Figure 1 and Figure 4, optical module 2 is arranged above heating module, figure It is arranged above optical module 2 as storing equipment, optical module 2 and test chamber 11 are arranged in cabinet 1, and image storage is set Standby, i.e., mobile phone 3 can be placed in cabinet 1, can also be directly placed on 1 upper surface of cabinet, when mobile phone 3 is placed on cabinet 1 Offer the through-hole of the camera observation for mobile phone 3 when on upper surface on cabinet 1 above test chamber 11, optical module setting exists 11 top of cabin is detected, optical module work can be directly controlled after heating module heats in sample, and then reduce sample It is mobile, while the overall volume of augmentation detection instrument can be reduced, save space.

In one preferred embodiment, as shown in figure 5, heating module includes heating copper post 51, heating copper post 51 is set For the internal copper post that several resistance wires are arranged, heating copper post 51 is to send out thermal property when being powered by resistance wire to be heated, by adding Hot 5 outer surface of copper post equably rejects heat in sample, and realization is evenly heated sample, and temperature is arranged on heating module It spends detection function (not embodied in figure), for controlling the temperature of heating copper post 51, temperature detecting function is by Logic control module 13 It controls, in the temperature detection result feedback to Logic control module 13 on heating module, the control to sample temperature may be implemented, It is reacted to sample amplification and isoperibol is provided, heating copper post 51 can be set one, several also can be set.

In one preferred embodiment, as shown in figure 5, heating module further includes the heating of covering heating copper post 51 Hot lid 5 heats hot lid 5 above heating copper post 51, heats hot lid 5 and be made of ito glass, heating hot lid 5 is led to by ito glass Thermal property is sent out when electric to be heated, and sample is placed in the hot lid 5 of heating when use, is heated the temperature of hot lid 5 generally than adding High 5 DEG C of temperature or so of hot copper post 51, can not only reduce evaporation and the thermal loss of sample, and can be improved add simultaneously Heating rate in thermal modules shortens the heating-up time, improves efficiency, other, which can be placed in, reduces thermal loss in cabinet 1 The hot lid 5 of heating can be substituted, then this is not repeated, heating hot lid 5 can be set into various shape, also can be set into various Size can be arranged according to sample size, not influence heating effect.

In one preferred embodiment, such as Fig. 1, radiator 6 is additionally provided in cabinet 1, radiator 6 is for giving Light emitting diode 21 (referring to Fig. 3) cooling and heat dissipation, guarantees that 21 temperature of light emitting diode is not too high, guarantees to a certain extent Light emitting diode 21 emits the stability of light intensity, improves the accuracy of the testing result of fluorescence detection module, and radiator 6 is set as Several cooling fins towards the light emitting diode 21, in addition to cooling fin, other existing radiators 6 also be can be used, This does not make too many restrictions.

The above-mentioned various embodiments enumerated can be combined with each other implementation, those skilled in the art under the premise of reconcilable In combination with attached drawing and above to the explanation of embodiment, as the foundation being combined to the technical characteristic in different embodiments.

It should be pointed out that the above is only the preferred embodiment of the utility model only, not to limit this reality With novel, within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on should all include It is within the protection scope of the utility model.

Claims (6)

1. a kind of portable augmentation detection instrument, it is characterised in that:

Including cabinet and it is arranged in the intracorporal detection cabin of case, battery, control module and fluorescence detection module；In the detection cabin Equipped with heating module, the heating module is electrically connected with the battery；

The fluorescence detection module includes the image for glimmering light activated optical module and for recording fluorescent reaction Store equipment；

The optical module includes the light emitting diode set gradually, optical filter, dichroscope and lens, the light emitting diode It is electrically connected with the battery；

The control module includes the mobile phone of logic control element and control logic control unit, the battery, the heating Module, the light emitting diode are electrically connected with the logic control element and are controlled by the logic control element；

The logic control element is set as single-chip microcontroller.

2. a kind of portable augmentation detection instrument according to claim 1, it is characterised in that:

The heating module includes heating copper post, and the heating copper post is set as the internal copper post that several resistance wires are arranged.

3. a kind of portable augmentation detection instrument according to claim 2, it is characterised in that:

The heating module further includes the heating heat lid of covering heating copper post, and the heating heat lid is made of ito glass.

4. a kind of portable augmentation detection instrument according to claim 1, it is characterised in that:

Radiator is equipped in the cabinet, the radiator is set as several cooling fins towards the light emitting diode.

5. a kind of portable augmentation detection instrument according to claim 1, it is characterised in that:

Described image stores the camera that equipment is the mobile phone.

6. a kind of portable augmentation detection instrument according to claim 5, it is characterised in that:

The optical module is arranged above heating module, and described image stores equipment and is arranged above the optical module.