CN209989394U - Novel fluorescence quantitative amplification detector - Google Patents
Novel fluorescence quantitative amplification detector Download PDFInfo
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- CN209989394U CN209989394U CN201920552451.4U CN201920552451U CN209989394U CN 209989394 U CN209989394 U CN 209989394U CN 201920552451 U CN201920552451 U CN 201920552451U CN 209989394 U CN209989394 U CN 209989394U
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Abstract
The utility model relates to a novel fluorescence quantitative amplification detector, which comprises a shell, a nucleic acid amplification module arranged in the shell, an XY axis linear module arranged at the bottom of the shell, at least one fluorescence detection module arranged at the output end of the XY axis linear module, and a control panel arranged on the front side of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescence detection module and the XY axis linear module and displaying detection data; the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, thermoelectric refrigerating sheets and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescence detection module and exposed out of the upper end of the shell; the utility model discloses a XY axle straight line module can drive fluorescence detection module and remove under the sample cell, directly carries out fluorescence detection to the sample cell bottom portion, is favorable to improving exciting light efficiency, obtains higher fluorescence sensitivity and fluorescence signal intensity for the testing result is more accurate.
Description
Technical Field
The utility model relates to a fluorescence detection field refers in particular to a novel fluorescence quantitative amplification detector.
Background
The nucleic acid amplification fluorescence detection system irradiates exciting light to a reagent in a sample tube, and then receives fluorescence instantaneously excited from the reagent in the sample tube to realize qualitative and quantitative detection of a sample.
At present, some fluorescent quantitative detection devices are also present in the market, for example, an optical excitation and detection system of a fluorescent quantitative PCR detector is disclosed in the prior art 201410199206.1, a rapid multi-channel real-time fluorescent quantitative detection device is disclosed in the prior art 201510031523.7, and a real-time fluorescent quantitative PCR gene amplification detector is disclosed in the prior art 201710286911.9, which all adopt a top detection mode, that is, excitation light is transmitted and refracted from the top of a sample tube into the sample tube through a top cover, and a generated fluorescent signal is reflected in the sample tube and then returned through the top cover of the sample tube for receiving, but the detection mode has high requirement on the light transmittance of the top cover of the sample tube, the light transmittance of the top cover of the sample tube seriously affects the sensitivity of sample detection, and when the distance is long, the light emission is weakened and the signal is weakened; for example, 201610152466.2 discloses a multi-fluorescence channel detection system for real-time fluorescence quantitative PCR, 201711310372.4 discloses a real-time fluorescence quantitative amplification detector, which adopts a side detection mode that excitation light enters a sample tube from the side of the sample tube through an optical fiber, and a generated fluorescence signal is reflected in the sample tube and then received by the same or another optical fiber on the side of the sample tube.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provide a novel fluorescence quantitative amplification detector.
In order to achieve the above purpose, the utility model adopts the technical scheme that: a novel fluorescence quantitative amplification detector comprises a shell, a nucleic acid amplification module arranged in the shell, an XY-axis linear module arranged at the bottom of the shell, at least one fluorescence detection module arranged at the output end of the XY-axis linear module, and a control panel arranged on the front surface of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescence detection module and the XY-axis linear module and displaying detection data; the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, a thermoelectric refrigerating piece and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescence detection module and exposed out of the upper end of the shell; and the upper end of the shell is also respectively provided with a shell cover which can be turned outwards and a spring lock catch used for fixing the shell cover.
Preferably, the fluorescence detection module comprises a box body, and a light source, an excitation optical filter, a dichroic mirror, a detection optical filter, a fluorescence detector and a reflection prism which are respectively arranged in the box body; the light source emits horizontal light, the horizontal light passes through the excitation light filter to generate excitation light with corresponding wavelength, the dichroic mirror and the excitation light are placed at an angle of 45 degrees, the excitation light is horizontally reflected to the reflection prism, the horizontal excitation light is reflected by the reflection prism and then vertically enters the bottom of the sample tube, the fluorescent substance in the sample tube is excited to generate fluorescence, part of the fluorescence is horizontally reflected to the dichroic mirror through the reflection prism, then enters the detection light filter through the dichroic mirror to filter out pure fluorescence, and the filtered fluorescence finally enters the fluorescence detector; and lenses are arranged between the light source and the excitation optical filter, between the detection optical filter and the fluorescence detector and between the reflection prism and the sample tube in the box body.
Preferably, thermoelectric refrigeration piece and radiator unit all are provided with two, and place long limit one side at the heating frame from inside to outside respectively.
Preferably, the upper end of the shell is provided with a groove; the shell cover can be arranged in the groove in an outward turning mode; the heating frame is exposed out of the bottom surface of the groove; the shell cover is also provided with a heat preservation cover with the shape corresponding to that of the heating frame.
Preferably, the front face of the housing is inclined upward.
Preferably, the inclination angle of the front face of the housing is 45 degrees.
Preferably, the two side surfaces of the shell are respectively provided with a heat dissipation window.
Preferably, the light source is one of an LED lamp, a xenon lamp, a halogen lamp, or a laser light source.
Preferably, the fluorescence detector is one of a CCD detector, a photodiode, or a PMT photomultiplier tube.
Because of above-mentioned technical scheme's application, compared with the prior art, the utility model have the following advantage:
1. the utility model discloses a XY axle straight line module can drive fluorescence detection module and move under the sample cell, directly carries out fluorescence detection to the sample cell bottom portion, does not need optic fibre to carry out signal transmission, not only is favorable to improving exciting light efficiency, obtains higher fluorescence sensitivity and fluorescence signal intensity for the testing result is more accurate, and can greatly reduced production and cost of maintenance;
2. the fluorescence detection module in the utility model can reflect the horizontal exciting light to vertically inject into the bottom of the sample tube by adding the reflecting prism, thereby greatly reducing the thickness of the box body and reducing the whole volume;
3. the utility model discloses well thermoelectric refrigeration piece and radiator unit all are provided with two, but rapid heating up or cooling to shorten check-out time, improve detection efficiency.
Drawings
The technical scheme of the utility model is further explained by combining the attached drawings as follows:
FIG. 1 is a schematic structural diagram of the novel fluorescence quantitative amplification detector of the present invention;
FIG. 2 is a schematic structural view of the present invention with the housing removed;
FIG. 3 is a schematic structural view of the fluorescence detection module of the present invention mounted on the XY-axis linear module;
FIG. 4 is a light path diagram of the fluorescence detection module of the present invention;
FIG. 5 is a circuit diagram of the fluorescence quantitative amplification detector of the present invention.
Wherein: 1. a control panel; 2. a housing; 3. a sample tube; 4. a groove; 5. a heat preservation cover; 6. a shell cover; 7. a heat dissipation window; 8. a nucleic acid amplification module; 81. a heating rack; 82. a sample through hole; 83. a thermoelectric refrigeration chip; 84. a heat dissipating component; 9. an XY axis linear module; 10. a spring lock catch; 11. a fluorescence detection module; 111. a box body; 112. an LED lamp; 113. a lens; 114. exciting the optical filter; 115. a reflective prism; 116. a dichroic mirror; 117. detecting the optical filter; 118. a CCD detector.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
FIGS. 1-5 show the novel fluorescence quantitative amplification detector of the present invention, which comprises a housing 2, a nucleic acid amplification module 8 disposed in the housing 2, an XY axis linear module 9 disposed at the bottom of the housing 2, two fluorescence detection modules 11 disposed side by side at the output end of the XY axis linear module 9 and having different wavelengths, and a control panel 1 disposed on the front side of the housing 2 for controlling the working states of the nucleic acid amplification module 8, the fluorescence detection modules 11 and the XY axis linear module 9 and displaying the detection data; the nucleic acid amplification module 8 comprises a heating frame 81 which is arranged above the fluorescence detection module 11 and is exposed out of the upper end of the shell 2, a plurality of sample through holes 82 which are arranged on the heating frame 81 in a matrix manner and used for placing the sample tubes 3, thermoelectric cooling sheets 83 which are respectively arranged on one side of the heating frame 81 and are arranged from inside to outside, and a heat dissipation assembly 84; the upper end of the shell 2 is also respectively provided with a shell cover 6 which can be turned outwards and a spring lock catch 10 used for fixing the shell cover 6; when the device is used, the sample tube 3 is inserted into the sample through hole 82 on the heating frame 81, the shell cover 6 is covered and fixed through the spring lock catch 10, then the device is controlled by the control panel 1, the XY-axis linear module 9 can drive the fluorescence detection module 11 with the required wavelength to move to the position under the sample tube 3, the fluorescence detection is directly carried out on the bottom of the sample tube 3, and finally the detection data is uploaded to the control panel 1 to be displayed.
Further, the fluorescence detection module 11 includes a box body, an LED lamp 112, an excitation filter 114, a dichroic mirror 116, a detection filter 117, a CCD detector 118, and a reflection prism 115, which are respectively disposed in the box body; the horizontal light emitted by the LED lamp 112 passes through the excitation filter 114 to generate excitation light with a corresponding wavelength, the dichroic mirror 116 is placed at an angle of 45 degrees with the excitation light, the excitation light is horizontally reflected to the reflection prism 115, the reflection prism 115 reflects the horizontal excitation light and then vertically emits the reflected horizontal excitation light to the bottom of the sample tube 3, the fluorescent substance in the sample tube 3 is excited to generate fluorescence, part of the fluorescence is horizontally reflected to the dichroic mirror 116 through the reflection prism 115, then the pure fluorescence is filtered out through the detection filter 117 by incidence of the dichroic mirror 116, and the filtered fluorescence is finally emitted to the CCD detector 118; the lenses 113 are arranged between the LED lamp 112 and the excitation filter 114, between the detection filter 117 and the CCD detector 118, and between the reflection prism 115 and the sample tube 3 in the box body, and light can be converged through the lenses 113, so that the measurement is more accurate.
Further, thermoelectric refrigeration piece 83 and radiator unit 84 all are provided with two, and respectively by inside toward placing in long limit one side of heating frame 81 outward, but rapid heating up or cooling to shorten check-out time, improve detection efficiency.
Further, a groove 4 is arranged at the upper end of the shell 2; the shell cover 6 can be arranged in the groove 4 in an outward overturning manner; the heating frame 81 exposes the bottom surface of the groove 4; and the shell cover 6 is also provided with a heat-insulating cover 5 with the shape corresponding to that of the heating frame 81, so that the heating or cooling speed is further increased.
Further, the front surface of the housing 2 is inclined upwards by 45 degrees, so that a user can operate the control panel 1 or read data from the control panel 1 conveniently.
Furthermore, the two side surfaces of the shell are respectively provided with a heat dissipation window 7, so that the heat dissipation and ventilation effects are achieved, and the heat dissipation effect is enhanced.
The above is only a specific application example of the present invention, and does not constitute any limitation to the protection scope of the present invention. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (9)
1. A novel fluorescence quantitative amplification detector is characterized in that: the device comprises a shell, a nucleic acid amplification module arranged in the shell, an XY-axis linear module arranged at the bottom of the shell, at least one fluorescence detection module arranged at the output end of the XY-axis linear module, and a control panel arranged on the front surface of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescence detection module and the XY-axis linear module and displaying detection data; the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, a thermoelectric refrigerating piece and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescence detection module and exposed out of the upper end of the shell; and the upper end of the shell is also respectively provided with a shell cover which can be turned outwards and a spring lock catch used for fixing the shell cover.
2. The novel fluorescence quantitative amplification detector according to claim 1, characterized in that: the fluorescence detection module comprises a box body, a light source, an excitation optical filter, a dichroic mirror, a detection optical filter, a fluorescence detector and a reflecting prism, wherein the light source, the excitation optical filter, the dichroic mirror, the detection optical filter, the fluorescence detector and the reflecting prism are respectively arranged in the box body; the light source emits horizontal light, the horizontal light passes through the excitation light filter to generate excitation light with corresponding wavelength, the dichroic mirror and the excitation light are placed at an angle of 45 degrees, the excitation light is horizontally reflected to the reflection prism, the horizontal excitation light is reflected by the reflection prism and then vertically enters the bottom of the sample tube, the fluorescent substance in the sample tube is excited to generate fluorescence, part of the fluorescence is horizontally reflected to the dichroic mirror through the reflection prism, then enters the detection light filter through the dichroic mirror to filter out pure fluorescence, and the filtered fluorescence finally enters the fluorescence detector; and lenses are arranged between the light source and the excitation optical filter, between the detection optical filter and the fluorescence detector and between the reflection prism and the sample tube in the box body.
3. The novel fluorescence quantitative amplification detector according to claim 1 or 2, characterized in that: thermoelectric refrigeration piece and radiator unit all are provided with two, and place long limit one side at the heating frame by inside toward outside respectively.
4. The novel fluorescence quantitative amplification detector according to claim 3, characterized in that: a groove is formed in the upper end of the shell; the shell cover can be arranged in the groove in an outward turning mode; the heating frame is exposed out of the bottom surface of the groove; the shell cover is also provided with a heat preservation cover with the shape corresponding to that of the heating frame.
5. The novel fluorescence quantitative amplification detector according to claim 4, wherein: the right side of the shell is obliquely placed upwards.
6. The novel fluorescence quantitative amplification detector according to claim 5, wherein: the positive inclination angle of casing is 45 degrees.
7. The novel fluorescence quantitative amplification detector according to claim 6, wherein: and heat dissipation windows are respectively arranged on the two side surfaces of the shell.
8. The novel fluorescence quantitative amplification detector according to claim 2, characterized in that: the light source is one of an LED lamp, a xenon lamp, a halogen lamp or a laser light source.
9. The novel fluorescence quantitative amplification detector according to claim 2, characterized in that: the fluorescence detector is one of a CCD detector, a photodiode or a PMT photomultiplier tube.
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| CN201920552451.4U CN209989394U (en) | 2019-04-23 | 2019-04-23 | Novel fluorescence quantitative amplification detector |
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| CN201920552451.4U CN209989394U (en) | 2019-04-23 | 2019-04-23 | Novel fluorescence quantitative amplification detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929751A (en) * | 2019-04-23 | 2019-06-25 | 苏州合惠生物科技有限公司 | A kind of novel fluorescence quantitative augmentation detection instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929751A (en) * | 2019-04-23 | 2019-06-25 | 苏州合惠生物科技有限公司 | A kind of novel fluorescence quantitative augmentation detection instrument |
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