CN213803792U - PCR optical test system and PCR instrument - Google Patents
PCR optical test system and PCR instrument Download PDFInfo
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- CN213803792U CN213803792U CN202022713450.9U CN202022713450U CN213803792U CN 213803792 U CN213803792 U CN 213803792U CN 202022713450 U CN202022713450 U CN 202022713450U CN 213803792 U CN213803792 U CN 213803792U
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Abstract
The utility model relates to a PCR optics test system and PCR appearance. A PCR optical test system comprising: a reaction tube; a light source opposite to the lower part of the reaction tube; the first light guide rod is arranged between the light source and the reaction tube; a detecting device opposite to a side of the reaction tube; and the second light guide rod is arranged between the reaction tube and the detection device. PCR instrument, including PCR optical test system as above. The PCR optical test system and the PCR instrument are used for qualitatively detecting the RNA of the specific virus, only a single reaction tube or two reaction tubes are used for carrying out reaction at a single temperature, and a light source can be directly emitted below the reaction tubes on a light path structure, so that the light transmittance around the reaction tubes and the cross-sectional area of reaction liquid are larger, and the fluorescent signals can be collected from the side surfaces of the reaction tubes. Compared with the traditional scheme, the structure is simpler.
Description
Technical Field
The utility model relates to a PCR reaction technical field especially relates to PCR optical test system and PCR appearance.
Background
PCR (Polymerase Chain Reaction) is a molecular biology technique for amplifying and amplifying specific DNA or RNA fragments, and can be regarded as specific DNA or RNA replication in vitro, and the greatest feature of PCR is that it can greatly increase trace amounts of DNA or RNA, and it has important application in the detection of DNA, bacteria or viruses.
However, the existing PCR optical test system for qualitative detection of specific viruses generally has the condition of complex structure and higher cost.
SUMMERY OF THE UTILITY MODEL
Therefore, it is necessary to provide a PCR optical test system and a PCR instrument for solving the problem of complicated structure of the conventional PCR optical test system.
A PCR optical test system comprising: a reaction tube; a light source opposite to a lower portion of the reaction tube; the first light guide rod is arranged between the light source and the reaction tube; a detecting device opposite to a side of the reaction tube; and the second light guide rod is arranged between the reaction tube and the detection device.
In one embodiment, the PCR optical test system further comprises a negative lens disposed between the light source and the second light guide rod.
In one embodiment, the PCR optical test system further comprises an optical filter disposed between the second light guide rod and the detection device.
In one embodiment, the PCR optical testing system further includes a fixing cylinder, the first light guiding rod is disposed in the fixing cylinder, and the light source is disposed in the fixing cylinder and located at an end of the first light guiding rod away from the reaction tube.
In one embodiment, a first annular limiting table is formed inside the fixed cylinder, the light source and the first light guide rod are respectively disposed on two sides of the first annular limiting table, and the first light guide rod is inserted into the fixed cylinder and abuts against the first annular limiting table.
In one embodiment, the PCR optical test system further includes an optical channel tube and a fixing member, the second light guide rod is disposed in the optical channel tube, and the detection device is fixed in the optical channel tube through the fixing member and located at an end of the second light guide rod away from the reaction tube.
In one embodiment, the inner wall of the end of the optical path channel tube away from the reaction tube is provided with an internal thread, and the fixing member is provided with an external thread which is in threaded connection with the internal thread.
In one embodiment, a second annular limiting table is formed inside the optical path channel tube, the second light guide rod is located on one side, close to the reaction tube, of the second annular limiting table, the detection device is located on one side, far away from the reaction tube, of the second annular limiting table, and the second light guide rod penetrates through the optical path channel tube and abuts against the second annular limiting table.
In one embodiment, the PCR optical test system further comprises an optical filter disposed between the second light guide rod and the detection device;
and a third annular limiting table is formed inside the optical path channel tube, and the optical filter is in limiting fit with the third annular limiting table.
A PCR instrument comprising a PCR optical test system as described above.
The PCR optical test system and the PCR instrument are used for qualitatively detecting the RNA of the specific virus, only a single reaction tube or two reaction tubes are used for carrying out reaction at a single temperature, and a light source can be directly emitted below the reaction tubes on a light path structure, so that the light transmittance around the reaction tubes and the cross-sectional area of reaction liquid are larger, and the fluorescent signals can be collected from the side surfaces of the reaction tubes. Compared with the traditional scheme, the structure is simpler.
Drawings
FIG. 1 is a schematic diagram of a conventional PCR optical test system;
fig. 2 is a schematic structural diagram of a PCR optical test system according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a PCR optical test system according to an embodiment of the present invention.
Detailed Description
In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.
The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
As shown in fig. 1, the conventional PCR optical test system generally has a confocal structure, and specifically includes a light source 1, a lens 2, a dichroic beam splitter 3, a diaphragm 4, and a detection device 5, and the structure is complex because a fluorescent substance is excited by coaxial illumination and stray light is filtered from collected fluorescence.
As shown in fig. 2, an embodiment of the present invention relates to a PCR optical test system, which includes a reaction tube 100, a light source 200, a first light guide rod 300, a second light guide rod 600, and a detection device 400.
The light source 200 is opposite to the lower portion of the reaction tube 100; the first light guide rod 300 is disposed between the light source 200 and the reaction tube 100; the detection device 400 is opposite to the side of the reaction tube 100; the second light guide rod 600 is disposed between the reaction tube 100 and the detection device 400.
In the above-mentioned PCR optical test system, the reaction tube 100 is used for loading a reaction solution (the reaction solution is a solution added with a substance to be tested, and the substance to be tested may be DNA or RNA, etc.), and after the environmental temperature of the reaction tube 100 is controlled by the temperature control unit, the substance to be tested in the reaction tube 100 can be rapidly propagated and increased for detection.
Further, after the substance to be detected is increased through breeding, a fluorescent qualitative detection substance capable of being adhered to the substance to be detected can be added into the reaction solution, and then the light source 200 is turned on, so that light emitted by the light source 200 is irradiated on the reaction solution through the first light guide rod 300, so that the fluorescent detection substance on the reaction solution emits light and is irradiated on the detection device 400 through the second light guide rod 600, and then detection is completed through the detection device 400.
Wherein the light source 200 may be an LED light source 200; the reaction tube 100 is an amplification tube; the first light guide rod 300 is used for converging the light emitted by the light source 200, ensuring the intensity of incident light and reducing the attenuation of the light emitted by the light source 200 in the air; the second light guide rod 600 is used for converging fluorescence, so that the intensity of the fluorescence is ensured, the attenuation of the fluorescence in the air is reduced, and the accuracy of a detection result is further ensured; the detector can adopt a small-area CCD (charge coupled device) chip or a photomultiplier, and can copy the substance to be detected for several times on the premise of qualitative measurement so as to achieve the detection capability of the photodiode.
The PCR optical test system is used for RNA qualitative detection of specific viruses, only a single reaction tube or two reaction tubes are used for carrying out reaction at a single temperature, and on the light path structure, a light source 200 can be directly irradiated below the reaction tube 100, the light transmittance around the reaction tube 100 and the cross-sectional area of reaction liquid are larger, so that the fluorescent signals can be collected from the side surface of the reaction tube 100. Compared with the traditional scheme, related lenses, dichroic beam splitters and other devices are omitted, and the structure is simpler.
In one embodiment, the PCR optical test system further comprises a negative lens 500, wherein the negative lens 500 is disposed between the light source 200 and the second light guide rod 600. The negative lens 500 is used to collect the fluorescence at a larger angle to ensure that more fluorescence impinges on the detection device 400. The negative lens 500 may be a concave lens or a negative focal length meniscus lens, the largest diameter of which does not exceed 10 mm.
Furthermore, the optical PCR test system further includes an optical filter 700, wherein the optical filter 700 is disposed between the second light guiding rod 600 and the detecting device 400. The optical filter 700 is used for filtering noise, the wavelength parameter of the optical filter changes with different fluorescent dyes, and the influence of the noise on the detection result is reduced, so that the accuracy of the detection result is ensured, the maximum diameter of the optical filter is not more than 10mm, and the thickness of the optical filter is generally between 1mm and 2 mm.
As shown in FIG. 3, in one embodiment, the PCR optical testing system further includes a fixing cylinder 800, the first light guide rod 300 is disposed in the fixing cylinder 800, and the light source 200 is disposed in the fixing cylinder 800 and located at an end of the first light guide rod 300 away from the reaction tube 100.
Specifically, the fixing cylinder 800 is used for fixing the first light guide rod 300 and the light source 200, so that the first light guide rod 300 and the light source 200 form an assembly, which is convenient to install.
More specifically, the fixed cylinder 800 is internally provided with a first annular limiting table, the light source 200 and the first light guide rod 300 are respectively arranged on two sides of the first annular limiting table, the first light guide rod 300 is arranged in the fixed cylinder 800 in a penetrating manner and is abutted against the first annular limiting table, the first annular limiting table has a limiting effect on the first light guide rod 300, and the first light guide rod 300 can be abutted against the first annular limiting table after being arranged in the fixed cylinder 800 in a penetrating manner, so that the installation and the installation are convenient.
Further, the PCR optical testing system further includes an optical channel tube 900 and a fixing member 410, the second light guiding rod 600 is disposed in the optical channel tube 900, and the detecting device 400 is fixed in the optical channel tube 900 through the fixing member 410 and is located at an end of the second light guiding rod 600 far away from the reaction tube 100.
Specifically, the optical channel tube 900 is used to fix the second light guiding rod 600 and the detection device 400, so that the second light guiding rod 600 and the detection device 400 form an assembly, which is convenient for installation.
Further, the fixing member 410 is disposed through an end of the optical path tube 900 far from the reaction tube 100, and seals an opening of the end of the optical path tube 900 far from the reaction tube 100 to shield light, so that the detection result of the detection device 400 is more accurate.
Specifically, the inner wall of the end of the optical path passage tube 900 away from the reaction tube 100 is provided with an internal thread, and the fixing member 410 is provided with an external thread, which is in threaded connection with the internal thread. Thus, the fixing member 410 can be installed, and the fixing member 410 is used for fixing the detection device 400, so that the detection device 400 is opposite to the end of the second light guiding rod 600 far away from the reaction light.
In one embodiment, the optical filter 700 is disposed in the optical channel tube 900 and located between the second light guiding rod 600 and the detection device 400, and the optical channel tube 900 is used to fix the second light guiding rod 600, the optical filter 700 and the detection device 400, so that the second light guiding rod 600, the optical filter 700 and the detection device 400 form an assembly.
Further, a second annular limiting table is formed inside the optical path channel tube 900, the first light guide rod 300 is located on one side, close to the reaction tube 100, of the second annular limiting table, the light filter 700 and the detection device 400 are located on one side, far away from the reaction tube 100, of the second annular limiting table, the second light guide rod 600 penetrates through the optical path channel tube 900 and is abutted against the second annular limiting table, the second annular limiting table has a limiting effect on the second light guide rod 600, and after the second light guide rod 600 penetrates through the optical path channel tube 900, the second light guide rod 600 can be abutted against the second annular limiting table, so that the fact that the second light guide rod is installed in place is indicated.
Further, a third annular limiting table is formed inside the optical path channel tube 900, and the optical filter 700 is in limiting fit with the third annular limiting table. After the optical filter 700 is mounted to the optical path passage tube 900, the optical filter 700 abuts against a third annular limiting table, and the third annular limiting table is used for limiting the position of the optical filter 700, thereby completing the mounting of the optical filter 700.
An embodiment is also directed to a PCR instrument comprising a PCR optical test system as described above.
In the above PCR instrument, the optical test system is used for qualitative detection of RNA of a specific virus, and only a single reaction tube 100 or two reaction tubes 100 are used to perform a reaction at a single temperature, and the light source 200 can be directly projected below the reaction tube 100 in the optical path structure, so that the light transmittance around the reaction tube 100 and the cross-sectional area of the reaction solution are increased, and thus, a fluorescent signal can be collected from the side surface thereof. Compared with the traditional scheme, related lenses, dichroic beam splitters and other devices are omitted, and the structure is simpler.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. A PCR optical test system, comprising:
a reaction tube;
a light source opposite to a lower portion of the reaction tube;
the first light guide rod is arranged between the light source and the reaction tube;
a detecting device opposite to a side of the reaction tube; and
and the second light guide rod is arranged between the reaction tube and the detection device.
2. The PCR optical test system of claim 1, further comprising a negative lens disposed between the light source and the second light guide rod.
3. The PCR optical test system according to claim 1, further comprising a filter disposed between the second light guiding rod and the detection device.
4. The optical PCR test system as claimed in claim 1, further comprising a fixing barrel, wherein the first light guide rod is disposed in the fixing barrel, and the light source is disposed in the fixing barrel and located at an end of the first light guide rod away from the reaction tube.
5. The PCR optical test system of claim 4, wherein a first annular limit table is formed inside the fixed cylinder, the light source and the first light guide rod are respectively disposed at two sides of the first annular limit table, and the first light guide rod is inserted into the fixed cylinder and abuts against the first annular limit table.
6. The optical PCR test system as claimed in claim 1, further comprising an optical channel tube and a fixing member, wherein the second light guide rod is disposed in the optical channel tube, and the detection device is fixed in the optical channel tube by the fixing member and located at an end of the second light guide rod away from the reaction tube.
7. The optical PCR test system as claimed in claim 6, wherein the inner wall of the optical path tube at the end away from the reaction tube is provided with an internal thread, and the fixing member is provided with an external thread, and the external thread is threadedly coupled with the internal thread.
8. The PCR optical test system according to claim 6, wherein a second annular position-limiting table is formed inside the optical channel tube, the second light guide rod is located on a side of the second annular position-limiting table close to the reaction tube, the detection device is located on a side of the second annular position-limiting table far away from the reaction tube, and the second light guide rod is inserted into the optical channel tube and abuts against the second annular position-limiting table.
9. The PCR optical test system according to claim 8, further comprising an optical filter disposed between the second light guiding rod and the detection device;
and a third annular limiting table is formed inside the optical path channel tube, and the optical filter is in limiting fit with the third annular limiting table.
10. A PCR instrument comprising the PCR optical test system according to any one of claims 1 to 9.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022713450.9U CN213803792U (en) | 2020-11-20 | 2020-11-20 | PCR optical test system and PCR instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022713450.9U CN213803792U (en) | 2020-11-20 | 2020-11-20 | PCR optical test system and PCR instrument |
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| CN213803792U true CN213803792U (en) | 2021-07-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093941A (en) * | 2022-06-25 | 2022-09-23 | 北京金诺美科技股份有限公司 | Reaction tube and fluorescence detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093941A (en) * | 2022-06-25 | 2022-09-23 | 北京金诺美科技股份有限公司 | Reaction tube and fluorescence detection device |
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Address after: Room 1401, headquarters building, Changsha Zhongdian Software Park Co., Ltd., 39 Jianshan Road, Changsha hi tech Development Zone, Changsha, Hunan 410000 Patentee after: HUNAN KAIQI SHIDAI BIOTECHNOLOGY CO.,LTD. Patentee after: Hunan Kaikai Times Technology Co.,Ltd. Address before: Room 1401, headquarters building, Changsha Zhongdian Software Park Co., Ltd., 39 Jianshan Road, Changsha hi tech Development Zone, Changsha, Hunan 410000 Patentee before: HUNAN KAIQI SHIDAI BIOTECHNOLOGY CO.,LTD. Patentee before: HUNAN UNSEAL TIMES ELECTRONICS INFORMATION TECHNOLOGY Co.,Ltd. |
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