CN103487375A - Three-color fluorescent detector based on white-light LED - Google Patents
Three-color fluorescent detector based on white-light LED Download PDFInfo
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- CN103487375A CN103487375A CN201310436904.4A CN201310436904A CN103487375A CN 103487375 A CN103487375 A CN 103487375A CN 201310436904 A CN201310436904 A CN 201310436904A CN 103487375 A CN103487375 A CN 103487375A
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Abstract
The invention discloses a three-color fluorescent detector based on a white-light LED. The white-light LED is used as an excitation light source; circular parallel beams with uniform brightness are obtained after white beams are collimated by a beam stray light filtering and shaping group; the irradiation of narrow-band exciting light to a test sample can be realized by a light splitting light path formed by a first color filter group, a color separation color filter group, a focusing lens group and a second color filter group; a narrow-band fluorescence signal, of which stray light is filtered, is received by the test surface of a photomultiplier. According to the fluorescent detector provided by the invention, the light source and light intensity sampled through a photodiode are used as signal reference of the test sample, so that three fluorescent dyes FITC, Cy<3+> and Cy<5+> can be detected simultaneously by the fluorescent detector, the entire detector is compact in structure, and has the characteristics of small volume, low power consumption, high sensitivity and portability, the semi-quantitative analysis to the fluorescence intensity of the test sample is achieved, and the detection sensitivity can meet a sodium fluorescein standard of 2.5 pg/ml.
Description
Technical field
The present invention relates to a kind of fluorescence detector that is applied to the biologic medical field.
Background technology
Fluoroscopic examination is mainly used in biochemical analysis, and especially molecular biological fluorescence signal detects.When Cucumber is excited by visible ray or UV-irradiation, can produce the utilizing emitted light longer than excitation wavelength, by measuring fluorescence intensity and the spectral range composition in can certain material of qualitative and quantitative analysis.
1852, Stokes has illustrated fluorescent emission mechanism: the photon collision of specific wavelength is to molecule, the molecule absorption photon, energy raises but is unstable, the energy that generally will absorb by release is got back to ground state, when excited state molecule with photon form emittance and while getting back to ground state, its light sent just is called fluorescence towards periphery.
The condition that fluorescence produces is that molecule must be got back to the lowest energy level of first excited state after the exciting light that absorbs the certain frequency scope by vibration relaxation, and downward radiation transistion just may produce fluorescence thus.Therefore, the most important condition that produces fluorescence is exactly to excite the light frequency must be consistent with the characteristic frequency of molecule, is exactly secondly that this molecule must have fluorescence efficiency to a certain degree.
Fluorescence efficiency, also claim fluorescence quantum yield, and it means the ability of material emitting fluorescence, and under the exciting light of same intensity, the quantum efficiency of fluorescence molecule is higher, and the fluorescence of emission is also just stronger.It is defined as the ratio of the fluorescent photon number launched after the material extinction and the photon number of the exciting light of absorption, that is:
The fluorescence intensity that fluorescent material is launched is relevant with several factors, comprises environmental factor (as temperature, pH value, ionic strength, with the covalency coupling of other molecule etc.), particularly below several factors:
1) excite light intensity.Generally, excite light intensity larger, the intensity of fluorescence is also larger, and both are linear relationship within the specific limits.But, under the irradiation of high strength exciting light long period, can cause that photobleaching (Photo bleaching) phenomenon or optical damage phenomenon (Photo destruction) to a certain degree occurs fluorescent material, reduced the intensity of fluorescence on the contrary.
2) quantum efficiency.Under the exciting light of same intensity, the quantum efficiency of fluorescence molecule is higher, and the fluorescence of emission is also just stronger.
3) absorptivity.Under the exciting light of same intensity, the absorptivity of fluorescent material is larger, and the fluorescence of emission is stronger.
4) dyeing concentration of fluorescent material.When the dyeing concentration of fluorescent material hangs down, fluorescence intensity and dyeing concentration are linear relationship basically, and when dyeing concentration is higher, fluorescent material there will be quencher (Quenching) phenomenon, has reduced on the contrary the intensity of fluorescence.
Summary of the invention
technical matters:the invention provides a kind of be applied to bioanalysis, compact conformation, but volume is little, low in energy consumption, highly sensitive and the three fluorescence detector of portability based on white light LEDs, can, under laboratory environment or Site Detection, realize detecting the semi-quantitative analysis of sample fluorescence intensity.
technical scheme:a kind of three fluorescence detector based on white light LEDs of the present invention, comprise the white LED light source group set gradually along input path, the filtering of light beam parasitic light and shaping group, the first color filter group, the dichronic mirror group, focus lens group, be positioned at the second color filter group and photomultiplier assembly on reflected light path, white LED light source group and the filtering of light beam parasitic light and shaping group are placed in shading cover, the white LED light source group, the filtering of light beam parasitic light and shaping group, the optical axis of the first color filter group and dichronic mirror group incident light axis overlap, focus lens group is positioned on the emitting light path of dichronic mirror group one side, the second color filter group and photomultiplier assembly are successively set on the emitting light path of dichronic mirror group opposite side, the optical axis of focus lens group and the second color filter group, the incidence window center of photomultiplier assembly all with the emission optical axis coincidence of dichronic mirror group.
In the present invention, the white LED light source group comprises semisphere white light LEDs, collimation plano-convex lens, 45
0reflection-type spectroscope, condenser lens and photodiode, the collimation plano-convex lens is arranged on the utilizing emitted light exit direction of semisphere white light LEDs, 45
0the spectroscopical incident light axis of reflection-type and the optical axis coincidence that collimates plano-convex lens, condenser lens and photodiode are successively set on 45
0spectroscope reflection direction below, and the optical axis of condenser lens and photodiode is all with 45
0the spectroscopical reflection optical axis of reflection-type overlaps.
In the present invention, the filtering of light beam parasitic light and shaping group comprise that numerical aperture is 0.4 ~ 0.5, the first convex lens of common optical axis and the second convex lens, the aperture between the first convex lens and the second convex lens, identical and the focus of the numerical aperture of the first convex lens and the second convex lens overlaps, aperture is arranged on the optical axis of the first convex lens and the second convex lens, and the center of aperture overlaps with the focus of the first convex lens and the second convex lens.
In the present invention, the first color filter group comprises support, is arranged on the first colour filter mirror disk, the first stepper motor and the first optoelectronic switch on support, the first colour filter mirror disk Shang Ju center equidistantly arranges three the first color filter, three the first color filter are respectively to 467 ~ 498nm, 513 ~ 556nm, the exciting light filtering veiling glare of 604 ~ 644nm wave band, the first stepper motor is connected with the first colour filter mirror disk and for driving its rotation, the selection of realization to different-waveband the first color filter, the first optoelectronic switch is for identifying the position of the first colour filter mirror disk.
In the present invention, the dichronic mirror group comprises support, is arranged on three hole color separation mirror disks, the second stepper motor and the second optoelectronic switch on support, three hole color separation mirror disk Shang Ju centers equidistantly arrange three dichronic mirrors, the transmitted light wave cutoff wavelength of three dichronic mirrors is respectively 506nm, 562nm, 660nm, the second stepper motor is connected with three hole color separation mirror disks and for driving its rotation, the selection of realization to different dichronic mirrors, the second optoelectronic switch is for identifying the position of three hole color separation mirror disks.
In the present invention, focus lens group comprises with incident light and becomes 45
0the reflective mirror arranged, be positioned at the 3rd convex lens that reflective mirror below focal length is 10 ~ 50mm and control the stepper motor of the 3rd convex lens upper and lower displacement, the optical axis of the 3rd convex lens overlaps with the reflected light direction of reflective mirror, the 3rd convex lens can move along optical axis direction under the control of stepper motor, realize focusing.
In the present invention, the second color filter group comprises support, is arranged on the second colour filter mirror disk, the 3rd stepper motor and the 3rd optoelectronic switch on support, the second colour filter mirror disk Shang Ju center equidistantly arranges three the second color filter, three the second color filter are respectively to 513 ~ 556nm, 570 ~ 613nm, the utilizing emitted light filtering veiling glare of 672 ~ 712nm wave band, the 3rd stepper motor is connected with the second colour filter mirror disk and for driving its rotation, the selection of realization to different-waveband the second color filter, the 3rd optoelectronic switch is for identifying the position of the second colour filter mirror disk.
In a specific embodiment of fluorescence detector of the present invention, excitation source adopts the constant-current circuit power supply, carrier with 96 orifice plates as the fluoroscopic examination sample, photomultiplier gathers the biological specimen fluorescence signal produced that is excited and is converted into voltage signal, photodiode Real-time Collection semisphere white light LEDs light intensity signal is converted into voltage signal simultaneously, above-mentioned voltage signal after low-pass filter as the input of differential amplifier, process the signal input analog-to-digital converter of output through differential amplifier, microprocessing unit carries out certain filtering algorithm processing to the digital signal after changing, obtain the raw data for experimental analysis.According to the detection signal decision method, further analyze, the form demonstration testing result with data or chart, complete the detection to biological sample.
beneficial effect:the present invention compared with prior art, has the following advantages:
1, this fluorescence detector adopts LED as excitation source, and the stable of the real-time light intensity of excitation source guaranteed in the constant-current source circuit power supply, and the light-intensity variation scope is below 0.5%.Contrast adopts laser instrument or halogen tungsten lamp as light source, LED has advantages of that volume is little, low in energy consumption, luminescence efficiency is high, the life-span is long, reduced heat that light source produces and the volume of corollary apparatus, thereby designed the light path of compact conformation, for microminiaturization and the portability of whole system lays the first stone.The compare late effect of light source sampling feedback circuit, the autonomous feedback regulation of constant current source hardware circuit, its constant output current, guarantee the stable of the real-time light intensity of excitation source.
2, due to light decay and the above-mentioned light-intensity variation of LED, the sample fluorescence signal value of photomultiplier collection also can fluctuate thereupon, causes experimental result to lack feasible degree and comparability.This fluorescence detector adopts the high sensitivity photodiode to sample to light source, its output voltage signal is as the reference of the output voltage signal of photomultiplier, processing by follow-up hardware road and algorithm, get rid of the impact of light-intensity variation on testing result, the testing result of guaranteeing experiment sample has and can quantize and comparability.
3, this fluorescence detector adopts step motor control the 3rd convex lens to move along optical axis direction, realization is carried out automatic focus to sample, during detection, adopt software algorithm to control the motor operation and carry out automatic focus, guarantee that detector has versatility for the sample of different volumes amount.
4, this fluorescence detector adopts 96 orifice plates as the carrier that detects sample, can realize disposable 96 hole scan samples being detected, and guarantees that it has high-throughout characteristics.
5, this fluorescence detector adopts light path and the Machine Design of compact conformation, high 30cm, 30cm, the 15cm of being respectively of whole device length and width, weight is 4kg, the characteristics of the large-scale heaviness of existing fluoroscopic examination quasi-instrument have been overcome, microminiaturization and the portability of whole instrument have been realized, not only be applicable to detect under the environment in laboratory, and can be used for Site Detection.For biomedical engineering field, there are high sensitivity, characteristics cheaply, promoted its development microminiaturized and portability, filled up the blank of biological medicine association area.
The accompanying drawing explanation
The three fluorescence detector plan structure schematic diagram that Fig. 1 is white light LEDs of the present invention.
The side-looking structural representation that Fig. 2 is the white LED light source group.
The side-looking structural representation that Fig. 3 is the filtering of light beam parasitic light and shaping group.
The structural representation that Fig. 4 is the first color filter group.
The structural representation that Fig. 5 is the dichronic mirror group.
Fig. 6 is focus lens group side-looking structural representation.
Fig. 7 is the second color filter group schematic diagram.
Light source sampling and power circuit schematic diagram that Fig. 8 is apparatus of the present invention
In figure, have: white LED light source group 1, the filtering of light beam parasitic light and shaping group 2, the first color filter group 3, dichronic mirror group 4, focus lens group 5, the second color filter group 6, photomultiplier assembly 7, base plate 8, shading cover 9, upper cover plate 10, semisphere white light LEDs 11, collimation plano-convex lens 12,45
0reflection-type spectroscope 13, photodiode 14, condenser lens 15, the first convex lens 21, the second convex lens 22, aperture 23, the first colour filter mirror disk 31, stepper motor 32, optoelectronic switch 33, the first color filter 34, support 35, color separation mirror disk 41, the second stepper motor 42, the second optoelectronic switch 43, dichronic mirror 44, reflective mirror 51, the 3rd convex lens 52, stepper motor 53, the second colour filter mirror disk 61, the 3rd stepper motor 62, the 3rd optoelectronic switch 63, the second color filter 64.
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Embodiment
Below in conjunction with Figure of description and embodiment, the present invention is done further and illustrates.
Consult Fig. 1, three fluorescence detector based on white light LEDs of the present invention, comprise white LED light source group 1, the filtering of light beam parasitic light and the shaping group 2, the first color filter group 3, dichronic mirror group 4, the focus lens group 5 that set gradually along input path, be positioned at the second color filter group 6 and photomultiplier assembly 7 on reflected light path,, also comprise base plate 8, shading cover 9 and upper cover plate 10.White LED light source group 1 and the filtering of light beam parasitic light and shaping group 2 are placed in shading cover 9, the optical axis of white LED light source group 1, the filtering of light beam parasitic light and shaping group 2, the first color filter group 3 and dichronic mirror group 4 incident light axis overlap, focus lens group 5 is positioned on the emitting light path of dichronic mirror group one side, 7 of the second color filter group 6 and photomultiplier groups are successively set on the emitting light path of dichronic mirror group 4 opposite sides, the optical axis of focus lens group 5 and the second color filter group 6, the incidence window center of photomultiplier assembly 7 all with the emission optical axis coincidence of dichronic mirror group 4.
In three fluorescence detector based on white light LEDs of the present invention, launched the white light of collimation by white LED light source group 1, white light beam, after the filtering of light beam parasitic light and shaping group 2, obtains the uniform circular parallel beam of luminance brightness; This light beam, after the first color filter group 3 filtering, sees through the exciting light of corresponding wave band; Colour separation filter group 4 minute surfaces and incident light are 45
0place at angle, to incident angle, is 45
0exciting light high anti-, fluorescence is thoroughly high; After above-mentioned exciting light projects colour separation filter group 4, be reflected to focus lens group 5, by focus lens group 5, focus on the to be detected biological sample tank that contains fluorochrome label; The fluorescence produced after the sample stimulated luminescence irradiates, be back to dichronic mirror group 4 places through the former road of focus lens group 5, and fluorescence sees through dichronic mirror group 4, and the exciting light mixed is reflected onto light source direction; The fluorescence seen through, through the second color filter group 6, further carries out filtering to veiling glare, obtains effective arrowband fluorescence signal; This fluorescence signal shines on the test surface of photomultiplier assembly 7, by photomultiplier, converts fluorescence signal to voltage signal.Photodiode 14 gathers semisphere white light LEDs 11 by 45 simultaneously
0the light intensity of reflection-type spectroscope 13 reflections, be converted into voltage signal.Above-mentioned voltage signal after low-pass filter as the input of differential amplifier, process the signal input analog-to-digital converter of output through differential amplifier, microprocessing unit carries out certain filtering algorithm processing to the digital signal after changing, and obtains the raw data for experimental analysis.According to the detection signal decision method, further analyze, the form demonstration testing result with data or chart, complete the detection to biological sample.Embodiment is as follows:
The first step, white LED light source group 1, the filtering of light beam parasitic light and shaping group 2, the first color filter group 3, dichronic mirror group 4, focus lens group 5, the second color filter group 6, photomultiplier assembly 7 all are fixed on base plate 8, the optical axis, the first color filter group 3 optical axises and dichronic mirror group 4 incident light axis that guarantee optical axis, the filtering of light beam parasitic light and the shaping group 2 of white LED light source group 1 overlap, and guarantee optical axis, dichronic mirror group 4 reflection optical axis, the second color filter group 6 optical axises and photomultiplier assembly 7 optical axis coincidences of focus lens group 5.
Second step, the white light of white LED light source group 1 emission collimation, white light is after the filtering of light beam parasitic light and shaping group 2, obtain the uniform circular light beam of light distribution, shading cover 9 coordinates with base plate 8, white LED light source group 1 and parasitic light filtering and shaping group 2 are covered, and circular light beam is penetrated by the circular hole of shading cover 9.
The 3rd step, outgoing beam, after the first color filter group 3, obtains the exciting light of corresponding wave band after filtering, and after projecting dichronic mirror group 4, in dichronic mirror group 4, the minute surface of three dichronic mirrors 44 and incident light are 45
0place at angle, and the transmitted light wave cutoff wavelength of three dichronic mirrors 44 is respectively 506nm, 562nm, 660nm, be less than this cutoff wavelength with regard to total reflection, equal to be greater than cutoff wavelength with regard to transmission.4 pairs of incident angles of dichronic mirror group are 45
0exciting light high anti-, fluorescence is thoroughly high, exciting light is transmitted to focus lens group 5, by focus lens group 5, exciting light is focused on to the biological specimen surface of containing fluorochrome label to be detected.
The 4th step, after the sample stimulated luminescence irradiates, produce fluorescence, be back to dichronic mirror group 4 places through the former road of focus lens group 5 again, preliminary filtering exciting light, fluorescence sees through dichronic mirror group 4, again through the second color filter group 6, further, to veiling glare filtering in fluorescence, obtain effective fluorescence signal, effectively fluorescence signal shines on the test surface of photomultiplier group 7.
The 5th step, computer software sends instruction to microcontroller by the mode of serial communication, controls on the one hand the required lens combination of stepper motor rotation choice experiment of the first color filter group 3, the second color filter group 6 and dichronic mirror group 4 through hardware circuit; The opposing party controls the unlatching of white LED light source group 1, photomultiplier group 7, white LED light source group 1 light intensity, the enlargement factor of programmable amplifier and the experiment parameters such as reading times of biological specimen through hardware circuit.Experimental actual needs, control electric machine rotation and configuration correlation parameter, realizes to the particular organisms sample fluoroscopic examination of particular detection condition.
The 6th step, provide power supply by constant-current source circuit for white LED light source group 1, guarantees the stable of the real-time light intensity of excitation source.Photomultiplier gathers the biological specimen fluorescence signal produced that is excited and is converted into voltage signal, photodiode Real-time Collection semisphere white light LEDs light intensity signal is converted into voltage signal simultaneously, above-mentioned voltage signal after low-pass filter as the input of differential amplifier, process the signal input analog-to-digital converter of output through differential amplifier, microprocessing unit carries out certain filtering algorithm processing to the digital signal after changing, and obtains the raw data for experimental analysis.According to the detection signal decision method, further analyze, the form demonstration testing result with data or chart, complete the detection to biological sample.
Further introduce apparatus of the present invention below by specific embodiment:
LED in white LED light source group 1 is the semisphere white light LEDs, and luminous power is 3W, and the focal length of its collimation lens is 12.7mm, and diameter is 12.7mm, and the reflectivity of catoptron is 4%, and the photosensitive area of photodiode is 3.6mm * 3.6mm; The focal length of two convex lens in the filtering of light beam parasitic light and shaping group 2 is as 20mm, and diameter is 12.7mm, and the hole diameter of aperture is 1mm; Three filter plate diameters of the first color filter group 3 are 12mm, and filter range is respectively 467 ~ 498nm, 513 ~ 556nm, 604 ~ 644nm; Divide three color separation film diameters penetrating mirror group 4 to be 15mm, light splitting cut-off optical wavelength is respectively 506nm, 562nm, 660nm; Three filter plate diameters of the second color filter group 6 are 12mm, and filter range is respectively 513 ~ 556nm, 570 ~ 613nm, 672 ~ 712nm; The argent catoptron that the mirror diameter of focus lens group 5 is 14mm, the diameter of condenser lens is 12.7mm, focal length is 12.7mm; PMT model in photomultiplier group 7 is CR194.With reference to above-mentioned embodiment, detection sensitivity can reach 2.5pg/ml uranin (standard).
Claims (7)
1. the three fluorescence detector based on white light LEDs, it is characterized in that, this detector comprises the white LED light source group (1) set gradually along input path, the filtering of light beam parasitic light and shaping group (2), the first color filter group (3), dichronic mirror group (4), focus lens group (5), be positioned at the second color filter group (6) and photomultiplier assembly (7) on reflected light path, described white LED light source group (1) and the filtering of light beam parasitic light and shaping group (2) are placed in shading cover (9), white LED light source group (1), the filtering of light beam parasitic light and shaping group (2), the optical axis of the first color filter group (3) and dichronic mirror group (4) incident light axis overlap, described focus lens group (5) is positioned on the emitting light path of dichronic mirror group (4) one sides, the second color filter group (6) and photomultiplier assembly (7) are successively set on the emitting light path of dichronic mirror group (4) opposite side, the optical axis of focus lens group (5) and the second color filter group (6), the incidence window center of photomultiplier assembly (7) all with the emission optical axis coincidence of dichronic mirror group (4).
2. the three fluorescence detector based on white light LEDs according to claim 1, is characterized in that, described white LED light source group (1) comprises semisphere white light LEDs (11), collimation plano-convex lens (12), 45
0reflection-type spectroscope (13), photodiode (14) and condenser lens (15), described collimation plano-convex lens (12) is arranged on the utilizing emitted light exit direction of semisphere white light LEDs (11), 45
0the incident light axis of reflection-type spectroscope (13) and the optical axis coincidence that collimates plano-convex lens (12), condenser lens (15) and photodiode (14) are successively set on 45
0spectroscope (13) reflection direction below, and the optical axis of condenser lens (15) and photodiode (14) is all with 45
0the reflection optical axis of reflection-type spectroscope (13) overlaps.
3. the three fluorescence detector based on white light LEDs according to claim 1, it is characterized in that, the filtering of described light beam parasitic light and shaping group (2) comprise that numerical aperture is 0.4 ~ 0.5, first convex lens (21) of common optical axis and the second convex lens (22), be positioned at the aperture (23) between described the first convex lens (21) and the second convex lens (22), identical and the focus of the numerical aperture of described the first convex lens (21) and the second convex lens (22) overlaps, aperture (23) is arranged on the optical axis of the first convex lens (21) and the second convex lens (22), and the center of aperture (23) overlaps with the focus of the first convex lens (21) and the second convex lens (22).
4. according to claim 1, 2 or the 3 described three fluorescence detectors based on white light LEDs, it is characterized in that, described the first color filter group (3) comprises support, be arranged on the first colour filter mirror disk (31) on described support, the first stepper motor (32) and the first optoelectronic switch (33), described the first colour filter mirror disk (31) Shang Ju center equidistantly arranges three the first color filter (34), described three the first color filter (34) are respectively to 467 ~ 498nm, 513 ~ 556nm, the exciting light filtering veiling glare of 604 ~ 644nm wave band, described the first stepper motor (32) is connected with the first colour filter mirror disk (31) and for driving its rotation, the selection of realization to different-waveband the first color filter (34), described the first optoelectronic switch (33) is for identifying the position of the first colour filter mirror disk (31).
5. according to claim 1, 2 or the 3 described three fluorescence detectors based on white light LEDs, it is characterized in that, described dichronic mirror group (4) comprises support, be arranged on three hole color separation mirror disks (41) on described support, the second stepper motor (42) and the second optoelectronic switch (43), described three hole color separation mirror disk (41) Shang Ju centers equidistantly arrange three dichronic mirrors (44), the transmitted light wave cutoff wavelength of described three dichronic mirrors (44) is respectively 506nm, 562nm, 660nm, described the second stepper motor (32) is connected with three hole color separation mirror disks (41) and for driving its rotation, the selection of realization to different dichronic mirrors (44), described the second optoelectronic switch (33) is for identifying the position of three hole color separation mirror disks (41).
6. according to claim 1, the 2 or 3 described three fluorescence detectors based on white light LEDs, it is characterized in that, described focus lens group (5) comprises with incident light and becomes 45
0the reflective mirror (51) arranged, be positioned at the 3rd convex lens (52) that reflective mirror (51) below focal length is 10 ~ 50mm and the stepper motor (53) of control the 3rd convex lens upper and lower displacement, the optical axis of described the 3rd convex lens (52) overlaps with the reflected light direction of reflective mirror (51), the 3rd convex lens (52) can move along optical axis direction under the control of stepper motor (53), realize focusing.
7. according to claim 1, 2 or the 3 described three fluorescence detectors based on white light LEDs, it is characterized in that, described the second color filter group (6) comprises support, be arranged on the second colour filter mirror disk (61) on described support, the 3rd stepper motor (62) and the 3rd optoelectronic switch (63), described the second colour filter mirror disk (61) Shang Ju center equidistantly arranges three the second color filter (64), described three the second color filter (64) are respectively to 513 ~ 556nm, 570 ~ 613nm, the utilizing emitted light filtering veiling glare of 672 ~ 712nm wave band, described the 3rd stepper motor (62) is connected with the second colour filter mirror disk (61) and for driving its rotation, the selection of realization to different-waveband the second color filter (64), described the 3rd optoelectronic switch (63) is for identifying the position of the second colour filter mirror disk (61).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872362A (en) * | 2017-01-18 | 2017-06-20 | 浙江大学 | LED light source device and its application for visible and near infrared spectrum detection |
| CN108572028A (en) * | 2017-03-10 | 2018-09-25 | 中国科学院物理研究所 | Photoelectric measuring device for low-dimensional quantum structure |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580746A (en) * | 2003-08-06 | 2005-02-16 | 北京航空航天大学 | Matrix type biochip CCD scanning fetch device |
| CN1724997A (en) * | 2005-07-12 | 2006-01-25 | 东华大学 | Fluorescence detection optical device with microflow control chip |
| CN101592659A (en) * | 2009-02-09 | 2009-12-02 | 马义才 | A kind of based on the test strip quantitative detection system and the method thereof that continue fluorescent-substance markers |
| US20110042581A1 (en) * | 2009-08-19 | 2011-02-24 | Shimadzu Corporation | Fluorescence Detector |
| CN102798621A (en) * | 2012-08-17 | 2012-11-28 | 中国科学院上海光学精密机械研究所 | Multi-piece reflection type ultraviolet induced biological fluorescence detection system |
| CN203443886U (en) * | 2013-09-24 | 2014-02-19 | 东南大学 | Three-color fluorescence detector based on white-light LED (Light-Emitting Diode) |
-
2013
- 2013-09-24 CN CN201310436904.4A patent/CN103487375A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580746A (en) * | 2003-08-06 | 2005-02-16 | 北京航空航天大学 | Matrix type biochip CCD scanning fetch device |
| CN1724997A (en) * | 2005-07-12 | 2006-01-25 | 东华大学 | Fluorescence detection optical device with microflow control chip |
| CN101592659A (en) * | 2009-02-09 | 2009-12-02 | 马义才 | A kind of based on the test strip quantitative detection system and the method thereof that continue fluorescent-substance markers |
| US20110042581A1 (en) * | 2009-08-19 | 2011-02-24 | Shimadzu Corporation | Fluorescence Detector |
| CN102798621A (en) * | 2012-08-17 | 2012-11-28 | 中国科学院上海光学精密机械研究所 | Multi-piece reflection type ultraviolet induced biological fluorescence detection system |
| CN203443886U (en) * | 2013-09-24 | 2014-02-19 | 东南大学 | Three-color fluorescence detector based on white-light LED (Light-Emitting Diode) |
Non-Patent Citations (2)
| Title |
|---|
| 卢健 等: "上转换发光免疫试纸条扫描检测系统研究", 《光子学报》 * |
| 卢健 等: "上转换发光免疫试纸条扫描检测系统研究", 《光子学报》, vol. 35, no. 4, 30 April 2006 (2006-04-30) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872362A (en) * | 2017-01-18 | 2017-06-20 | 浙江大学 | LED light source device and its application for visible and near infrared spectrum detection |
| CN106872362B (en) * | 2017-01-18 | 2023-12-12 | 浙江大学 | LED light source device for visible and near infrared spectrum detection and application thereof |
| CN108572028A (en) * | 2017-03-10 | 2018-09-25 | 中国科学院物理研究所 | Photoelectric measuring device for low-dimensional quantum structure |
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Application publication date: 20140101 |