CN104568799A - Monochromatic light composited light scanning type luminosity absorption detection system - Google Patents
Monochromatic light composited light scanning type luminosity absorption detection system Download PDFInfo
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Abstract
The invention discloses a monochromatic light composited light scanning type luminosity absorption detection system. The monochromatic light composited light scanning type luminosity absorption detection system is characterized in that a detection light source is a red, green and blue three-primary-color monochromatic source, the light intensity of the red, green and blue three-primary-color monochromatic source is adjusted under the control of a light source driving module, a collimation unit is adopted for light path collimation, and then fusion is carried out to generate detecting light in different wave bands; the working time sequence of the light source driving module is controlled to enable the detection light source to output visible light sequence used as detection light according to a red, orange, yellow, green, blue-green, blue and purple sequence, the detecting light is projected to a sample to be detected in a visible light sequence mode; and a single photoelectric sensor is arranged and is used for receiving transmission light rays of the sample to be detected in a visible light sequence mode, the transmission light rays are subjected to data processing by a data processing unit, and a visible light absorption spectrogram of the sample to be detected can be acquired. According to the monochromatic light composited light scanning type luminosity absorption detection system, the three-primary-color monochromatic source is adopted to replace a white light source in the traditional spectrophotometer, the driving voltage combination is adjusted to generate the spectrogram, the power consumption is low, the luminous effect is stable and the volume is small.
Description
Technical field
The present invention relates to photometric absorbance detection system, absorb sensitive materials based on lambert-Beer law at visible-range interior focusing degree and carry out examination and analysb, be applied to the fields such as biological and chemical metering, water quality and food analysis, medical science and drug inspection.
Background technology
Spectrophotometric method is the detection analytical technology of a kind of routine in chemical industry, medicine, material and environmental area, and it is simple to operate, highly sensitive, good reliability, and most of Check and Inspection laboratory is all equipped with the spectrophotometer of different levels.
When photon acts on material molecule, if when the energy of photon is equal with the energy difference of certain energy inter-stage of material molecule, photon by energy transferring to material molecule, valence electron in molecule transits to excited electronic state from electronic ground state after obtaining energy, simultaneous vibration, the energy level variations of rotating.The structure of different material molecule is different, and the distribution of its molecular entergy level is also different, so the photon wavelength absorbed is also different, Here it is material is to the selective absorbing of light.Absorption photometry is the analytical approach set up the selective absorbing of light based on material, have sensitive, accurate, quick, selectivity is good, be applicable to the advantage such as microcomponent mensuration.
If measure something to the monochromatic degree of absorption of different wave length, take wavelength X as horizontal ordinate, absorbance A is ordinate, can obtain an absorption curve or absorption spectrum, and reflection something is to the monochromatic degree of absorption of different wave length.Different material has different absorption spectrums, so can provide the information of qualitative analysis.And for same substance, concentration c is different, also different to the absorption intensity of special wavelength light, so absorption intensity can provide the information of quantitative test.
When a beam intensity is I
0monochromatic light vertical irradiation something solution after, because a part of light is absorbed, therefore the intensity of transmitted light is down to I, then the transmittance T of solution is defined as: T=I/I
0, absorptivity A is defined as: A=-lgT, that is: T=10
-A.According to lambert-Beer law: A=κ bc, wherein: the concentration that b is solution layer thickness, c is solution, κ are absorptivity, the person's character of absorptivity and solution, the factor such as temperature and wavelength are relevant.It can thus be appreciated that when solution layer thickness b and absorptivity κ fixes, the concentration of absorbance A and solution is linear.When quantitative test, first need measure solution to the absorbing state of different wavelengths of light, therefrom determine maximum absorption wavelength, then with the light of this wavelength for light source, measure the absorbance A of a series of concentration known c solution, make A-c concentration absorption curve.When analyzing unknown solution, according to the absorbance A measured, look into concentration absorption curve and can determine corresponding concentration, this is the ultimate principle of metric measurement concentration.
As can be seen here, the monochromatic light utilizing optical splitter to obtain purity higher in spectrophotometric method is a key.Spectrophotometer of the prior art complex light is decomposed into the spectrum being applicable to detecting.Visible spectrophotometer generally uses tungsten lamp, and tungsten lamp sends the complex light of 400 nanometer ~ 760 nano wave lengths, is obtained the continuous spectrum in visible-range by optical splitter, and arranges photoelectric sensing array and detect.But on the one hand because the instability of tungsten light source reduces photometric measuring accuracy, on the other hand, the setting of light splitting optical path and photoelectric sensing array causes system architecture complicated, easily produces error.
Summary of the invention
The present invention is the weak point for avoiding existing for above-mentioned prior art, there is provided a kind of with the photometric absorbance detection system of the photosynthetic photoscanning of monochrome, by Systematical control three primary light source combination producing different-waveband light, extinction spectrogram is obtained by the mode of scanning, to avoid complicated light splitting light modulation step and expensive photoelectric sensing detection arrays, while effectively completing the scanning of extinction spectrogram, reduce the cost of photometric absorbance pick-up unit.
The present invention is that technical solution problem adopts following technical scheme:
The present invention is with the photometric absorbance detection system of the photosynthetic photoscanning of monochrome, and its design feature is:
Arranging detection light source is adopt red, green, blue three primary colours monochromatic source, the luminous intensity of described red, green, blue three primary colours monochromatic source is regulated under the control of light source driver module, beam path alignment is carried out by collimation unit, and through merging the detection light generating different-waveband; Control the work schedule of described light source driver module, make described detection light source using scanning mode according to red, orange, yellow, green, blue, blue, purple Sequential output visible light sequential as detection light, described detection light is projected to sample with the form of its visible light sequential;
Arrange single photoelectric sensor, described single photoelectric sensor receives the transmitted ray of sample by the form of sequence, utilizes data processing unit to carry out data processing for described transmitted ray, obtains the visible absorption spectrogram of sample.
The present invention is also with the design feature of the photometric absorbance detection system of the photosynthetic photoscanning of monochrome: described collimation unit is assembled by the three primary light source in described red, green, blue three primary colours monochromatic source to be fused on same optical axis.
The present invention is also with the design feature of the photometric absorbance detection system of the photosynthetic photoscanning of monochrome: described single photoelectric sensor is photoresistance, photodiode, phototriode, silicon photo diode, CMOS (Complementary Metal Oxide Semiconductor) light receiving semiconductor CMOS, charge coupled cell CCD or photomultiplier PMT.
The present invention is also with the design feature of the photometric absorbance detection system of the photosynthetic photoscanning of monochrome: described red, green, blue three primary colours monochromatic source is separate three primary light source, or the three primary light source be packaged together.
Compared with the prior art, beneficial effect of the present invention is embodied in:
1, the present invention utilizes red, green, blue three primary light source to replace white light source in traditional spectrophotometer, high-brightness light emitting diode can be directly adopted to form three primary light source, by regulating driving voltage combination producing spectrum, it is low in energy consumption, stable luminescence, compact.
2, in traditional spectrophotometer because arranging photosensor arrays, not only need photoelectric sensing array and light-splitting device proper calibration, and needing the consistance ensureing each photoelectric sensing module performance in array, this has very high requirement to the manufacture of system and assembly technology.Adopt single photoelectric sensor to carry out photoelectric signal transformation in present system, ensure that the consistance of performance, be conducive to increasing effective photoelectric sensing region, improve the stability in faint environment and reliability.Meanwhile, single photoelectric sensing module is adopted also greatly to be conducive to assembling and the calibration of system.
3, present system structure is simple and clear, do not need the parts such as wide spectrum light source, light splitting light modulation device, photoelectric sensing array, by adopting ultra-compact, super low-power consumption components and parts to implement, volume, weight reduction can be effectively reduced, being conducive to the instant detection system of the super portable photometric scanning of exploitation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 A, Fig. 2 B, Fig. 2 C and Fig. 2 D are the performance characterization that the present invention is applied to the detection of carmine photometric absorbance.
Number in the figure: 1 three primary colours monochromatic source; 2 collimation unit; 3 samples; 4 photoelectric sensors; 5 data processings and control module; 6 light source driver modules.
Embodiment
See Fig. 1, in the present embodiment with the version of the photometric absorbance detection system of the photosynthetic photoscanning of monochrome be:
Arranging detection light source is adopt red, green, blue three primary colours monochromatic source 1, the luminous intensity of described red, green, blue three primary colours monochromatic source 1 is regulated under the control of light source driver module 6, beam path alignment is carried out by collimation unit 2, and through merging the detection light generating different-waveband; Control the work schedule of described light source driver module 6, make described detection light source using scanning mode according to red, orange, yellow, green, blue, blue, purple Sequential output visible light sequential as detection light, described detection light is projected to sample 3 with the form of its visible light sequential.
Arrange single photoelectric sensor 4, described single photoelectric sensor 4 receives the transmitted ray of sample 3 by the form of sequence, utilize data processing unit to carry out data processing, obtain the visible absorption spectrogram of sample for described transmitted ray.
In concrete enforcement, collimation unit 2 is assembled by the three primary light source in described red, green, blue three primary colours monochromatic source 1 to be fused on same optical axis.Single photoelectric sensor 4 is photoresistance, photodiode, phototriode, silicon photo diode, CMOS (Complementary Metal Oxide Semiconductor) light receiving semiconductor CMOS, charge coupled cell CCD or photomultiplier PMT; Red, green, blue three primary colours monochromatic source 1 is separate three primary light source, or the three primary light source be packaged together.
In system shown in Figure 1, red, green, blue three primary colours monochromatic source 1 adopts high-brightness LED lamp, red, green and blue three kinds of primary light source are encapsulated in same lamp pearl by LED, generate pulse-width signal by data processing and control module 5, control the luminous intensity of each primary light source.Red, green, blue three coloured light line passes through collimation unit 2 by the light collection of scattering to central axis, and realizes the fusion of three primary colours component at central axis.Utilize data processing and control module 5 can regulate the intensity of each primary color component easily, control three primary colours proportionate relationship, the spectral components required for synthesis.On9658 illumination module selected by photoelectric sensor 4, and it is responsive at visible-range, and the light signal received is converted to electric signal.
Utilize present system and carry out the detection of being correlated with according to lambert-Beer law.
Experimentation:
Reagent: new coccine, deionized water.
Step 1, prepare carmine sample solution: take 30.2mg new coccine sample and be placed in 100mL beaker, add 40mL deionized water, use stirring rod to be stirred to color and no longer change, after solution-stabilized, solution is moved in 50mL volumetric flask, with water constant volume.This solution is the cochineal solution of 1mol/L.
Step 2, preparation blank and sample chromophoric solution: from 50mL volumetric flask, pipette 30uL respectively, the carmine sample solution of 60uL, 90uL, 120uL, 150uL is in the cuvette containing 3mL deionized water, and in corresponding cuvette, the concentration of cochineal solution is respectively 0.01mol/L, 0.02mol/L, 0.03mol/L, 0.04mol/L, 0.05mol/L.First is reference with blank solution, utilizes Hitachi U5100 spectrometer, measures the absorbance of each strength solution at 380-780nm.
Step 3, utilizing system in the present embodiment, take blank solution as reference, the solution to be measured in five cuvettes successively in measuring process 2, obtains each solution and is closing the absorbance that in light photometer, red, orange, yellow, green, blue, blue, purple seven kinds of color light are corresponding.
Step 4, curve plotting: record cochineal solution and see Fig. 2 A in the absorbance of visible spectrum and the working curve under closing light photometer, wherein curve a1, a2, a3, a4 and curve a5 respectively corresponding concentration be 0.01mol/L, 0.02mol/L, 0.03mol/L, 0.04mol/L, 0.05mol/L cochineal solution; The absorption photometric value of each concentration famille rose that Fig. 2 B to be Fig. 2 A medium wavelength be 508nm place is corresponding.
Fig. 2 C illustrates, under red, orange, yellow, green, blue, blue, purple seven kinds of color effect of light under present system setting, sensor is along with the increase of concentration, receive the change curve of light intensity, wherein curve c1, c2, c3, c4 and curve c5 respectively corresponding concentration be the cochineal solution of 0.01mol/L, 0.02mol/L, 0.03mol/L, 0.04mol/L and 0.05mol/L; Fig. 2 D illustrates, under the green glow of present system setting irradiates, and the light absorption value of variable concentrations cochineal solution in cuvette.
More than experiment can be found out, the working curve of present system has good linear relationship, and by known to contrasting of the light absorption value of solution with photometer, the present invention can meet the requirement of detection.In concrete enforcement, detection platform determines the sensitivity spectrum of material to be detected by initialize routine, selects insensitive spectrum to eliminate ground unrest and interference as the interior mark of detection, improves the sensitivity detected.
Present system can be widely used in biological and chemical metering, water quality and food analysis, medical science and drug inspection etc.
Claims (4)
1., with a photometric absorbance detection system for the photosynthetic photoscanning of monochrome, it is characterized in that:
Arranging detection light source is adopt red, green, blue three primary colours monochromatic source (1), the luminous intensity of described red, green, blue three primary colours monochromatic source (1) is regulated under the control of light source driver module (6), beam path alignment is carried out by collimation unit (2), and through merging the detection light generating different-waveband; Control the work schedule of described light source driver module (6), make described detection light source using scanning mode according to red, orange, yellow, green, blue, blue, purple Sequential output visible light sequential as detection light, described detection light is projected to sample (3) with the form of its visible light sequential;
Single photoelectric sensor (4) is set, described single photoelectric sensor (4) receives the transmitted ray of sample (3) by the form of sequence, utilize data processing unit to carry out data processing for described transmitted ray, obtain the visible absorption spectrogram of sample.
2. according to claim 1 with the photometric absorbance detection system of the photosynthetic photoscanning of monochrome, it is characterized in that: described collimation unit (2) is assembled by the three primary light source in described red, green, blue three primary colours monochromatic source (1) to be fused on same optical axis.
3. according to claim 1 with the photometric absorbance detection system of the photosynthetic photoscanning of monochrome, it is characterized in that: described single photoelectric sensor (4) is photoresistance, photodiode, phototriode, silicon photo diode, CMOS (Complementary Metal Oxide Semiconductor) light receiving semiconductor CMOS, charge coupled cell CCD or photomultiplier PMT.
4. according to claim 1 with the photometric absorbance detection system of the photosynthetic photoscanning of monochrome, it is characterized in that: described red, green, blue three primary colours monochromatic source (1) is separate three primary light source, or the three primary light source be packaged together.
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| CN105136684A (en) * | 2015-08-14 | 2015-12-09 | 上海蓝怡科技股份有限公司 | Multi-sample detection device and method |
| CN108196730A (en) * | 2018-01-18 | 2018-06-22 | 安徽三弟电子科技有限责任公司 | A kind of multi-functional laser virtual keyboard system |
| CN115494003A (en) * | 2022-10-09 | 2022-12-20 | 马鞍山市桓泰环保设备有限公司 | Sewage multi-monitoring-factor online monitoring system |
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