CN103217392A - Food safety inspection device and food safety inspection method of photoelectric colorimetry based on colored light detector - Google Patents
Food safety inspection device and food safety inspection method of photoelectric colorimetry based on colored light detector Download PDFInfo
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- CN103217392A CN103217392A CN2013101299995A CN201310129999A CN103217392A CN 103217392 A CN103217392 A CN 103217392A CN 2013101299995 A CN2013101299995 A CN 2013101299995A CN 201310129999 A CN201310129999 A CN 201310129999A CN 103217392 A CN103217392 A CN 103217392A
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Abstract
The invention discloses a food safety inspection device of photoelectric colorimetry based on a colored light detector. The device comprises a light source, a colorimetric tank, a light sensor inductor and a signal processing and controlling module, wherein the light source is located at one side of the colorimetric tank; the light source is used for generating multi-wavelength compound light and the multi-wavelength compound light penetrates through the colorimetric tank; liquid to be detected is contained in the colorimetric tank; the light sensor inductor is used on the other side of the colorimetric tank; the light sensor inductor is used for receiving the compound light which penetrates through the colorimetric tank, and converting received light signals into R (Red), G (Green) and B (Blue) electric signals; the signal processing and controlling module is connected with the control module and the light sensor inductor, and is used for processing electric signals sent out by the light sensor inductor, then calculating the absorbance of a solution according to the electric signals, and calculating the concentration or the content of substances according to the absorbance. The food safety inspection device disclosed by the invention is good for removing background noises and avoids interference of sample substrates.
Description
Technical field
The present invention relates to a kind of colorimetric analysis device, relate to a kind of more specifically with the detection method of colorama detector as the concentration of predetermined substance in the photoelectric colorimetry device of photoelectric sensor and the solution.Especially food safety detection device and detection method.
Background technology
The essential structure of common photoelectric colorimetric device is: light source, monochromatic light generating means, colorimetric pool, photoelectric sensor, signal Processing output and control module.
Light source irradiation produces the monochromatic light of specific wavelength behind the monochromator, monochromatic light shines on the light activated element of photoelectric sensor after seeing through colorimetric pool (in detected solution is arranged), the photelectric receiver action, light signal is become electric signal output, analytical calculation through signal Processing and control module, can calculate the absorbance or the absorbance changing value of detected solution, thereby calculate the concentration of certain food security composition in the detected solution.As Fig. 1, photoelectric colorimetry apparatus structure synoptic diagram.
Existing photoelectric colorimetry device, the colorimetric analysis experiment according to different needs a monochromatic device that can produce different centre wavelengths, and monochromator commonly used at present has modes such as the grating beam splitting of employing and narrow band pass filter group, but has following shortcoming respectively:
The first, adopting the characteristics of the monochromatic source generating means of grating original paper is the homogeneous beam that can obtain different centre wavelengths continuously, but its complicated in mechanical structure, wavelength repeatability is poor, and cost is higher.
Second, the combination of employing halogen tungsten lamp and narrow band pass filter also can obtain the homogeneous beam of specific wavelength, though it is simpler relatively than optical grating construction, but when needs obtain the monochromatic light of different wave length, still need to dispose respectively different optical filters, and these optical filters will be fixed on the wavelength switching physical construction of forming by step motor, filter wheel and annex by one, and optical filter is subjected to intrinsic technogenic influence, under wet environment, occur easily going mouldy, influence the light path performance.
Summary of the invention
The purpose of this invention is to provide a kind of with the photoelectric colorimetry device and the concentration detection method of colorama detector as photoelectric sensor, especially food safety detection device and detection method, simplifying photoelectric colorimetry device light channel structure, and overcome conventional photoelectric sensor and have comparatively complicated problems of physical construction.
For achieving the above object, the present invention is with traditional photoelectric colorimetry device: light source-monochromator-colorimetric pool-photoelectric sensor (what have also may be light source-colorimetric pool-monochromator-photoelectric sensor) structure, be reduced to light source-colorimetric pool-photo-detector sensor structure, the monochromatic light generating means is not set.It is characterized in that comprising light source, colorimetric pool, photo-detector inductor, signal Processing and control module.Described light source is positioned at a side of colorimetric pool, described light source is used to produce the multi-wavelength complex light, described multi-wavelength complex light is passed colorimetric pool, hold testing liquid in the described colorimetric pool, described photo-detector inductor is positioned at the colorimetric pool opposite side, described photo-detector inductor is used to receive the multi-wavelength complex light and is separately converted to R through the transmitted light behind the colorimetric pool and with the optical signal transmissive that receives, G, the electric signal of three passages of B, described signal Processing links to each other with the photo-detector inductor with control module, described signal Processing and control module are used to handle the electric signal that the photo-detector inductor sends, and calculate solution absorbency value in the colorimetric pool (or absorbance changing value) according to electric signal, calculate the concentration of target-finding material at last again.The colorama detector comprises the photo-detector of CCD, CMOS or mixed structure.
Further, described light source is the long complex light light source (white light source that comprises halogen tungsten lamp) of all-wave of the semiconductor laser light resource more than two kinds or led light source or visible light wave range in the redgreenblue.
Further, the colorama detector element is used as photoelectric sensor, this colorama detector element has the passage of exporting R, G, three kinds of unlike signals of B respectively, and R, the G in each picture point of photo-detector, B inductor be the wavelength of corresponding different peak value conversion ratios respectively.
Further, with traditional photoelectric colorimetry device: light source-monochromator-colorimetric pool-photoelectric sensor structure is reduced to light source-colorimetric pool-photo-detector sensor structure.
The present invention also proposes the detection method of specific substance concentration in a kind of solution (or content): the multi-wavelength complex light that adopts white light or multi wave length illuminating source to send shines directly into colorimetric pool; The multi-wavelength complex light projects on the colorama detector after seeing through colorimetric pool; Three kinds of unlike signals of R, G, B of colorama detector output are gathered and handled to signal Processing and control module respectively; According to one or more wavelength that needs when measuring are used, use the standard substance of peak absorbance should wavelength being arranged, promptly having that the narrow band pass filter of corresponding absorption peak or standard solution are demarcated the output signal value of R, G, B and the calibration value that obtains; Calculate in the colorimetric pool solution to absorbance under the specific wavelength or absorbance changing value; Calculate at last the content or the concentration of certain material in the liquid according to absorbance or absorbance changing value.
Further, calibration process is: the one or more wavelength that need use during to measurement, with to the standard substance of peak absorbance should wavelength be arranged, be the logical solution of light that the light of one or more single wavelength or the narrow band pass filter with corresponding absorption peak see through to standard substance, to R, G, the output signal value of B is demarcated and is obtained calibration value: standard substance is put into colorimetric pool, after the described monochromatic light irradiation, measure the R that see through this moment, G, the B signal value, and with the baseline signal value that zeroing the time the measures calculating of comparing, calculate R respectively, G, the conversion ratio coefficient of B sensor under different wave length, and obtain f(R+G+B)=the middle a of a%*f (red)+b%*f (gree)+c%*f (blue), b, the conversion ratio coefficient value of c is to determine R, G, weight coefficient during the signal value of B under this wavelength calculates.
Light source is white light or the color light source that comprises halogen tungsten lamp.Narrow band pass filter is used for white light source.
Further, in the calibrating procedure, adopt one or more groups standard substance to repeat above-mentioned demarcating steps as required, to obtain at different absorbance scope internal conversion rate coefficients with different absorption curves.
For same substance, adopt the photo-detector original paper of different model in the calibrating procedure, the conversion ratio coefficient needs to demarcate again.
The present invention is based on because R, G, the B inductor of colorama detector are different to the photoelectric conversion rate of different wave length, electrical signal intensity by measuring R, G, each road of B respectively and mutual intensity rate, can calculate the spectral energy that shines certain particular range of wavelengths on the photo-detector, quantitative according to lambert Bill again, can extrapolate in the colorimetric pool solution to the absorbance under this wavelength (or absorbance changing value), and finally calculate the concentration of certain predetermined substance in the liquid (as nitrite, borax, melamine etc.).
Further, by reference wavelength and two different wave lengths of predominant wavelength are measured, predominant wavelength adopts the absorption peak wavelength of target detection thing, the absorbance that predominant wavelength is measured is the absorbance total value of target detection thing and substrate, reference wavelength employing and predominant wavelength is the wavelength of complementary color each other, what reference wavelength was measured is the absorbance of substrate, and accurate target detects the thing absorbance to obtain more with predominant wavelength absorbance total value deduction reference wavelength absorbance during calculating.LASER Light Source is made corresponding configuration.Light source is for comprising halogen tungsten lamp or light emitting diode white light source.
Beneficial effect of the present invention: the present invention need not to be provided with separately monochromator, has simplified light channel structure, has improved reliability, has reduced manufacturing cost.And can only export one road photosignal value with photoelectric sensors such as traditional photomultiplier, silicon photocells compares, the colorama detector can be exported three kinds of photosignal values of R, G, B of corresponding different wave length respectively simultaneously, help by adopting two kinds of differences and measuring solution absorbency simultaneously for the wavelength of complementary colors, can effectively remove background noise after comparing, avoid the substrate in the sample to disturb.The present invention is based on the photoelectric colorimetry device of colorama detector, adopt the redgreenblue semiconductor laser light resource.By material to the measurement result of the comprehensive more accurate quantitative response of the variation of the absorption of the light of every kind of wavelength to material.Results measured of the present invention is that the measurement result to milk is
Description of drawings
Fig. 1 tradition photoelectric colorimetry apparatus structure synoptic diagram
Fig. 2 photoelectric colorimetry apparatus structure of the present invention synoptic diagram
Embodiment:
Below in conjunction with accompanying drawing the present invention is done further explanation.
As shown in Figure 2, the multi-wavelength complex light that light source sends shines directly into colorimetric pool, and projects on the colorama detector sensor after seeing through colorimetric pool.Gather R, G, the B signal of the output of colorama detector respectively by signal Processing and control system, after analog to digital conversion and data processing, can obtain the photoelectric conversion signal value of R, G, B corresponding wavelength respectively, adopting special-purpose analysis software that these R, G, B signal value are carried out analyzing and processing, to calculate the absorbance (or absorbance changing value) of detected solution under a certain specific wavelength.Be that example illustrates concrete Measurement and analysis process with the weight analysis method below.
(1) zeroing: (in the colorimetric analysis experiment, absorbance that it is generally acknowledged distilled water is zero to distilled water in the colorimetric pool.) return to zero, measure R, G, the B output signal value of this moment respectively, as the reference value of zero absorbance.
(2) demarcate: the one or more wavelength that need use during to measurement, with to should the standard substance (narrow band pass filter or standard solution with corresponding absorption peak) of peak absorbance being arranged to R by wavelength, G, the output signal value of B is demarcated, standard substance is put into colorimetric pool, measure the R of this moment, G, the B signal value, and with the baseline signal value that zeroing the time the measures calculating of comparing, calculate R respectively, G, the conversion ratio coefficient of B sensor under different wave length, be f(R+G+B)=the middle a of a%*f (red)+b%*f (gree)+c%*f (blue), b, the coefficient value of c is to determine R, G, weight coefficient during the signal value of B under this wavelength calculates.As in the determination experiment of nitrite (colour developing product absorption peak wavelength is 535nm in the determination experiment), the weight coefficient of measuring through calibration experiment is respectively: a=14, b=65, c=21; And for example in the determination experiment of borax (determination experiment colour developing product absorption peak is 450nm), the weight coefficient of measuring through calibration experiment is respectively: a=3, b=9, c=88, in different determination experiments, can adopt one or more groups standard substance to repeat above-mentioned demarcating steps as required with different absorption curves, to obtain more accurate weight coefficient value in different absorbance scopes, for same substance, adopt the photo-detector original paper of diverse ways and different model, weight coefficient will have several differences.Obtain weight coefficient by knowing to the demarcation before the photo measure of the solution of material.
(3) measure: according to the peak absorbtivity wavelength of the required mensuration material of experiments of measuring, select corresponding measurement wavelength, software with the RGB weight coefficient substitution computing formula of this wavelength correspondence, records total rgb light value of electrical signals automatically.And, calculate the absorbance (or absorbance changing value) of detected solution, and go out the concentration of the target substance of required mensuration in the liquid in prediction on such basis with respect to a certain wavelength period according to langbobier law.
The above only is a preferred implementation of the present invention; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention; can also be according to different target-finding materials and different reagent; and the photo-detector original paper of different manufacturers and model, make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. based on the photoelectric colorimetry device of colorama detector, it is characterized in that comprising light source, colorimetric pool, photo-detector inductor, signal Processing and control module; Described light source is positioned at a side of colorimetric pool, described light source is used to produce the multi-wavelength complex light, described multi-wavelength complex light is passed colorimetric pool, hold testing liquid in the described colorimetric pool, described photo-detector inductor is used for the colorimetric pool opposite side, described photo-detector inductor is used to receive transmission and crosses the complex light of colorimetric pool and the light signal that receives is separately converted to R, G, B three road electric signal, described signal Processing links to each other with the photo-detector inductor with control module, described signal Processing and control module are used to handle the electric signal that the photo-detector inductor sends, calculate solution absorbency according to electric signal then, and calculate the concentration or the content of material in view of the above.
2. based on the photoelectric colorimetry device of colorama detector, it is characterized in that described light source is semiconductor laser light resource more than two kinds or led light source in the redgreenblue, comprises the white light source of halogen tungsten lamp.
3. colorama detector according to claim 1 is as the photoelectric colorimetry device of photoelectric sensor, it is characterized in that: the colorama detector element is used as photoelectric sensor, this colorama detector element has the passage of exporting R, G, three kinds of unlike signals of B respectively, and R, the G in each picture point of photo-detector, B inductor be the wavelength of corresponding different peak value conversion ratios respectively.
4. according to claim 1 with the photoelectric colorimetry device of colorama detector as photoelectric sensor, it is characterized in that: with traditional photoelectric colorimetry device: light source-monochromator-colorimetric pool-photoelectric sensor structure is reduced to light source-colorimetric pool-photo-detector sensor structure.
5. based on the absorbency detection method of colorama detector, it is characterized in that: the multi-wavelength complex light that adopts white light or multi wave length illuminating source to send shines directly into colorimetric pool; The multi-wavelength complex light projects on the colorama detector after seeing through colorimetric pool; Three kinds of unlike signals of R, G, B of colorama detector output are gathered and handled to signal Processing and control module respectively; According to one or more wavelength that needs when measuring are used, use the standard substance of peak absorbance should wavelength being arranged, promptly having that the narrow band pass filter of corresponding absorption peak or standard solution are demarcated the output signal value of R, G, B and the calibration value that obtains; Calculate in the colorimetric pool solution to absorbance under the specific wavelength or absorbance changing value; Calculate at last the content or the concentration of certain material in the liquid according to absorbance or absorbance changing value.
6. the absorbency detection method based on the colorama detector according to claim 5, it is characterized in that, calibration process is: the one or more wavelength that need use during to measurement, with to the standard substance of peak absorbance should wavelength be arranged, be the logical solution of light that the light of one or more single wavelength or the narrow band pass filter with corresponding absorption peak see through to standard substance, to R, G, the output signal value of B is demarcated and is obtained calibration value: standard substance is put into colorimetric pool, after the described monochromatic light irradiation, measure the R that see through this moment, G, the B signal value, and with the baseline signal value that zeroing the time the measures calculating of comparing, calculate R respectively, G, the conversion ratio coefficient of B sensor under different wave length, and obtain f(R+G+B)=the middle a of a%*f (red)+b%*f (gree)+c%*f (blue), b, the conversion ratio coefficient value of c is to determine R, G, weight coefficient during the signal value of B under this wavelength calculates.
7. the absorbency detection method based on the colorama detector according to claim 5, it is characterized in that in the calibrating procedure, adopt one or more groups standard substance to repeat above-mentioned demarcating steps as required, to obtain at different absorbance scope internal conversion rate coefficients with different absorption curves.
8. the absorbency detection method based on the colorama detector according to claim 5 is characterized in that in the calibrating procedure for same substance, adopts the photo-detector original paper of different model, and the conversion ratio coefficient is demarcated again.
9. the absorbency detection method based on the colorama detector according to claim 5, it is characterized in that by reference wavelength and two different wave lengths of predominant wavelength are measured, predominant wavelength adopts the absorption peak wavelength of target detection thing, the absorbance that predominant wavelength is measured is the absorbance total value of target detection thing and substrate, reference wavelength employing and predominant wavelength is the wavelength of complementary color each other, what reference wavelength was measured is the absorbance of substrate, and accurate target detects the thing absorbance to obtain more with predominant wavelength absorbance total value deduction reference wavelength absorbance during calculating.
10. the absorbency detection method based on the colorama detector according to claim 5 is characterized in that described light source is semiconductor laser light resource more than two kinds or led light source in the redgreenblue, comprises the white light source of halogen tungsten lamp.
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Application publication date: 20130724 |