CN106066317A - Optical chopper using method in delayed luminescence measurement system - Google Patents
Optical chopper using method in delayed luminescence measurement system Download PDFInfo
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- CN106066317A CN106066317A CN201610409306.1A CN201610409306A CN106066317A CN 106066317 A CN106066317 A CN 106066317A CN 201610409306 A CN201610409306 A CN 201610409306A CN 106066317 A CN106066317 A CN 106066317A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
Abstract
A kind of method that chopper is applied to photoluminescence measurement, may be used for the measurement of delayed luminescence, has two light paths: light path one is the light path of excitation light irradiation sample in measurement；Light path two is the light path that detector detection sample is luminous；The method utilizes illuminator or prism wherein one or two light paths to be reflected or refraction, makes two light paths all pass through same chopper.Thus achieve the luminescence the most simultaneously to exciting light and sample of the same chopper and carry out chopping the light, thus delayed luminescence and the lifetime of excited state of sample can be detected.The method may be used for spectrogrph and fluoroscopic imaging device, it is possible to arbitrary stable light source of arranging in pairs or groups uses, and without being equipped with pulse generator, delay generator and fast fitting of door etc. again, simplify apparatus and reduce cost.The method can also be arranged in pairs or groups powerful near infrared laser, for upper converting material or the time-resolved imaging of near infrared emission material.
Description
Technical field
The present invention relates to the using method of optical chopper, the method can apply to Instantaneous Spectrometer and time-resolved
In imaging device.Belong to optical instrument and manufacture field.
Background technology
In luminescence generated by light phenomenon, molecule is excited by illumination and transits to excited state, and the molecule of excited state can be by release light
Son returns to ground state, i.e. the fluorescence of molecule or phosphorescence.There is the life-span in the excited state of molecule, i.e. the molecule of excited state will be when crossing one section
After between, just release photon returns to ground state, and the lifetime of excited state of different molecular is the most different, and the molecular-excited state life-span is the longest, its
Lighting time interval is the longest.Under normal circumstances, the fluorescence lifetime of molecule in nanosecond, phosphorescent lifetime can reach Microsecond grade with
On.Delayed fluorescence molecule, its life-span can also reach Microsecond grade even Millisecond.Some phosphorescent molecules developed in recent years, the longevity
Life can reach second level.In addition some inorganic nano materials, its life-span is more than Millisecond.These long-life luminescences are referred to as
Delayed luminescence, also referred to as aura.
In the measurement of fluorescence and phosphorescence, it is generally required to build two light paths: light path one is the light of excitation light irradiation sample
Road, the light i.e. sent from light source, through the parts post-concentration such as lens, grating to sample, its objective is to make the molecule in sample be subject to
Illumination excites；Light path two is the light path that detector detection sample is luminous, and i.e. sample is luminous arrives after the parts such as lens, grating
Detector.During the measurement of stable state, light path one and light path two are opened, under conditions of i.e. exciting light irradiates sample always simultaneously
Measuring, this mode can obtain the information of sample luminescent spectrum and intensity, but can not obtain the letter in sample excitation state life-span
Breath.
Along with the reach of science, the excited state research of molecule more comes the most important in basic science and detection application.In order to
Obtaining the information in molecular-excited state life-span and time-resolved spectrum, people have developed Instantaneous Spectrometer.Instantaneous Spectrometer, including
Transient absorption and transient state fluorescence spectrophotometer, have important application in the photochemical research of optical physics.(referenced patent prospectus
CN201310392018.6, CN200510092520.0, CN201180017387.6, CN201110005032.7 etc..) for reality
Existing time-resolved measurement, can use light-pulse generator to excite sample, utilize simultaneously have high time resolution photomultiplier tube,
Single photon counter or there is the CCD of gate control function measure its absorption postponed or luminous signal, to obtain molecular-excited state
The information such as life-span.But in order to realize high temporal resolution, this kind of spectrogrph generally need to be equipped with expensive pulse and swash
Light device and CCD.And laser instrument is single wavelength, it is impossible to realize full wave measurement.The laser instrument of differently configured wavelength is permissible
Part solves this problem, but but causes the cost of instrument to be greatly increased.In order to reduce cost, optical chopper is applied by people
In the measurement of transient state spectrum.
Optical chopper is called for short chopper, the light that continuous light source can be sent, and is modulated into the optical signal of pulse or alternation.
(referenced patent prospectus CN200710025960.3, CN201310342971.X, CN200410093016.8, China is specially
Profit application number 201520771676.0 etc..) tradition one of chopper critical piece is chopper disk, is also chopper blade, on chopper disk
It is dispersed with the distribution in a center of symmetry centered by axle center of several or multiple light hole, generally these light holes.When motor controls to cut
CD, under certain rotating speed, continuous light source, after the light hole on chopper disk, is i.e. modulated into the periodicity arteries and veins of certain frequency
Wash off.
In some applications, a chopper can be added after the light path of the exciting light of spectrogrph, thus by the light of stable state
Source becomes the transient state light source of simulation, adds a chopper again before detector simultaneously, is used for regulating time delay with fast
The door time, to realize time-resolved measurement.Same scheme may be used for the design of time-resolved fluorescence microscope.(ginseng
Examine document Anal. Chem. 2011,83,2294-2300.) and have important application in bio-imaging field.
But in above-mentioned solution, need two choppers are carried out Collaborative Control, or Collaborative Control pulsed light
Source and chopper, owing to existing chopper typically uses electromechanics to modulate, its precision is affected relatively big by motor speed, and it is adjusted
The light-pulse generator made, per se with regular hour error, adds and necessarily to make error add two chopper Synchronization Control
Greatly, the temporal resolution of overall measurement is reduced.The precision improving single chopper can improve the time precision of overall measurement,
But the cost of chopper increases therewith.
Summary of the invention
In order to solve the problems referred to above, and reducing scattered light interference, the present invention has developed the usage of traditional chopper, has passed through structure
Build optical path, make the luminescence the most simultaneously to exciting light and sample of the same chopper carry out chopping the light, i.e. realize
Twice chopping the light, thus realize the Collaborative Control that pulse signal occurs and postpones detection.Owing to only using a chopper, thus phase
For two choppers of Collaborative Control, greatly improve time precision, and reduce the cost of spectrogrph.
Chopper used by this invention uses motor modulation, as general chopper, containing a chopper disk, chopping the light
Light hole is distributed on dish.When chopper disk is positioned in light path, chopper disk rotates and can make light path repeatedly in the state of on an off.
When light hole forwards in light path, i.e. light path is opened, otherwise, light path is closed.
The usage of the present invention is: is applied in a photoluminescence measurement system by a chopper, wherein has two light
Road: light path one is the light path of excitation light irradiation sample, its objective is to make the molecule in sample be excited by illumination；Light path two is detection
The light path that device detection sample is luminous, i.e. sample luminescence arrive detector.Utilize illuminator or prism by wherein one or two
The reflection of bar light path or refraction, make two light paths all pass through the chopper disk of this chopper, but the not coordination on chopper disk
Put.
Thus achieve the luminescence the most simultaneously to exciting light and sample of the same chopper and carry out chopping the light:
Be equivalent to stable light source to be transformed into light-pulse generator to the chopping the light of light path one；The chopping the light of light path two is equivalent to detector shutter
Folding.Owing to two light paths are different through the position of chopper disk, thus there is time difference in twice chopping the light, thus this measurement system can
Delayed luminescence and lifetime of excited state with detection sample.
In the method chopper is loaded in fluorescence spectrophotometer, builds suitable light path, it is possible to achieve sample delay
Luminous measurement.In such fluorescence spectrophotometer, it is glimmering that it contains general stable state such as light source, specimen holder or sample room, detector etc.
The basic parts of light instrument, possibly together with a chopper and several prism or illuminator.Additionally, the light path of this luminoscope is:
The light that light source sends is through the chopper disk of chopper, and as excitation light irradiation sample, then the light of sample is through prism or anti-
The reflection of light microscopic again passes by chopper disk, arrives detector.
In light path, it is also possible to collocation grating, lens, optical filter, light splitting piece, attenuator etc., it is also possible to increase dichroic
Mirror, prism and illuminator are to change the direction of light path.As long as in Ce Lianging, light source has first passed through chopper, sample before irradiating sample
The luminescence of product arrives detector after chopper again, then the principle measuring delayed luminescence is just as.
In the measurement of delayed luminescence, when light source is irradiated on sample by the light hole of chopper disk when, chopper disk
Another part has just blocked the sample light path to detector；Light source is made to be blocked to the light path of sample when chopper disk rotates
Time, the light hole on chopper disk has the most also turned over an angle around axle so that the luminescence of sample can be by this light hole
Arrive detector, be equivalent to shutter opening and collect optical signal.Signal collected by once irradiating is more weak, can make chopping the light
Dish uniform rotation, the most above-mentioned measurement process, and the signal of accumulation repetitive measurement, to obtain preferable signal intensity.
In addition to the measurement of spectrum, this method can be also used for fluoroscopic imaging device, for time-resolved imaging.?
In such device, it contains the basic of the general steady state imaging device such as light source, specimen holder or sample room, photographing unit or detector
Parts, possibly together with a chopper and several prism or illuminator.The light path of this imaging device is: the light warp that light source sends
Cross the chopper disk of chopper, as excitation light irradiation sample, then the light of sample through prism or illuminator reflection again
Through chopper disk, arrive detector.
In light path, it is also possible to collocation grating, lens, optical filter, light splitting piece, attenuator etc., it is also possible to increase dichroic
Mirror, prism and illuminator are to change the direction of light path.As long as in Ce Lianging, light source has first passed through chopper, sample before irradiating sample
The luminescence of product arrives photographing unit or detector after chopper, and the principle of the most time-resolved imaging is just as.
This device for time-resolved imaging (imaging of time gate, or postpone imaging), its principle with above prolong
The principle of the measurement of tardy light is consistent, is all, by a chopper, light source and sample luminescence are carried out chopping the light simultaneously: to light source
Chopping the light, can be converted into light-pulse generator by light source；To sample luminescence chopping the light, be equivalent to control time delay and aperture time.By
It is to be carried out by a chopper disk in twice chopping the light, places the position of sample, make incident illumination and emergent light not at same time warp
Cross light hole, i.e. may insure that twice chopping the light staggers the most completely.Thus in this approach, even if motor speed is slightly
Instability, makes frequency deviation occur, takes multiple measurements and does not also have light leakage phenomena, i.e. entirely eliminated backscatter light
Interference.Relative to using two choppers to control pulsed light and shutter respectively, greatly improve the signal to noise ratio of detection.Additionally, by
In the method, shutter control is controlled Tong Bu with chopping the light, thus in postponing spectrum and time-resolved imaging applications, it is not necessary to again
It is equipped with pulse generator, delay generator and fast fitting of door etc., simplifies apparatus and reduce cost.
Knowable to the principle of this test, this spectrogrph arbitrary stable light source of can arranging in pairs or groups uses, such as mercury lamp, xenon lamp etc.,
Relative to the pulse laser of use single wavelength, its excitation wavelength can use grating to regulate, and scope covers wide, can test
The material of different excitation wavelengths, greatly reduces cost.The method can also be arranged in pairs or groups powerful near infrared laser, as
980nm, 808nm etc., may be used for the time-resolved imaging of upper converting material or near infrared emission material.
Accompanying drawing explanation
Fig. 1 is for postponing spectral measurement device structural representation, and 101 is chopper disk, and 102 is light hole, and 103 is rotary shaft,
104 is sample cell, and 105 is reflecting prism.
Fig. 2 is the principle of chopper disk delay measurements, and 201 is chopper disk, and 202 is light hole.
Fig. 3 is for postponing image device structure schematic diagram, and 301 is chopper disk, and 302 is light hole, and 303 is lens, and 304 is anti-
Light microscopic, 305 is dichroic mirror, and 306 is sample room, and 307 is sample, and 308 is lens, and 309 is detector.
Fig. 4 is the microscopic structure schematic diagram postponing imaging, and 401 is chopper disk, and 402 is light hole, and 403 is illuminator,
404 is filter, and 405 is dichroic mirror, and 406 is object lens, and 407 is specimen holder, and 408 is sample, and 409 is filter.
Fig. 5 is the microscopic structure schematic diagram postponing imaging, and 501 is chopper disk, and 502 is light hole, and 503 is filter, 504
For dichroic mirror, 505 is object lens, and 506 is specimen holder, and 507 is sample, and 508 is filter, and 509 is detector.
Fig. 6 is the inverted microscope structural representation postponing imaging, and 601 is chopper disk, and 602 is light hole, and 603 for filtering
Mirror, 604 is specimen holder, and 605 is sample, and 606 is object lens, and 607 is illuminator, and 608 is filter lens, and 609 is illuminator, and 610 are
Lens, 611 is detector.
Detailed description of the invention
In order to principle and its advantage of the present invention are described, below by specific embodiment, the present invention is made further
Illustrate, its object is to help and be better understood from present disclosure, but these specific embodiments limit never in any form
Protection scope of the present invention.In actual applications, most suitable scheme can be implemented as the case may be.
Embodiment 1, chopper measures delayed luminescence for fluorescence spectrophotometer.
As it is shown in figure 1, be optical path schematic diagram, containing a fan-shaped light hole 102 on chopper disk 101, exciting light leads to
Crossing chopper disk to be irradiated on sample cell 104, sample sends fluorescence or phosphorescence, a portion fluorescence or phosphorescence after being stimulated
After the reflection of total reflection prism 105, again pass by chopper disk 101, be detected by the detector afterwards.
In this light path, the sample light that exciting light goes out with prismatic reflection through the position of chopper disk is through chopper disk position
Different.The principle of this measurement device delayed luminescence is as illustrated in fig. 2, it is assumed that exciting light arrives through some B through some A, sample luminescence
Detector, chopper disk 201 uniform rotation clockwise, accordingly, light hole 202 rotates clockwise around axle, can be according to this through A, B point
The position at place.
Being a cycle from time t1 to t5, the position forwarded to according to light hole is different, is roughly divided into and excites, postpones, surveys
Amount, four states of closedown.Within the time period of t1 to t2, it is in excited state, in the range of now A point is positioned at light hole, the most sharp
The luminous light hole 202 that can pass through on chopper disk 201 irradiate sample, although now sample is luminous, but B point is cut by chopper disk
Disconnected, light can not pass through, thus detector can't detect signal.Chopper disk 201 rotates clockwise, within the time period of t2 to t3,
Being in delaying state, A, B point is not the most in the range of light hole, and excitation light irradiation is less than sample, but sample returns the most immediately
To ground state, continue radioluminescence, when chopper disk continues to rotate clockwise, within the time period of t3 to t4, be in measuring state, B
Point falls in the range of light hole, and being equivalent to shutter opens, and the most now luminescence of sample can be detected by the detector, and works as chopper disk
Continuing to rotate clockwise, within the time period of t4 to t5, be closed, A, B point is not the most in the range of light hole, though sample
So continue radioluminescence, but excited state decays complete substantially, and do not detected by detector.
In above process, exciting light, sample be luminous, the state of shutter changes over as in figure 2 it is shown, t1 and t5 time
Between difference for cycle time, the in fact cycle of chopper disk chopping the light, the time difference of t1 and t2 is the time of pulsed light photograph, equal to chopping the light
The pulsewidth of dish chopping the light gained pulsed light, the time difference of t2 and t3 is time delay, and with the position of A, B 2, and pulsewidth is relevant,
Under conditions of pulsewidth is constant, change the position of A, B 2, thus it is possible to vary time delay, when the time difference of t3 and t4 is shutter
Between, i.e. measure the time, equal to the pulsewidth of chopper disk chopping the light gained pulsed light, the i.e. time difference equal to t1 and t2.
Knowable to said process, pulse, delay, shutter are controlled by a chopper disk, during multi-cycle measurement, i.e.
Make chopper disk rotary speed unstabilization, and make frequency shake, as long as determining the position of A, B 2, it can be ensured that be pulse, delay, fast
Door occurs according to this, outside making sample scattered light be blocked in, reduces the interference that non-delayed is luminous.
Knowable to this principle, the method is not limited to use the single hole chopper disk in Fig. 1, and porous chopper disk can also reach same
The measurement effect of sample.
The frequency assuming a chopper disk chopping the light is 2.5 kHz, and pulse duty factor is 0.25, then the cycle is 0.4ms, pulsewidth
Being 0.1ms with aperture time, incident illumination is different with emergent light position, and time delay, within 0.2ms, can be easy to survey
The amount life-span is at the sample of more than 0.1ms.
These samples have phosphorescent metal complex, long-life delayed fluorescence molecule, rare earth nano material etc..
The sample that life-span to be measured is shorter, can improve the frequency of chopper, or use more highly sensitive detector.
If chopper chopper frequency is 250 kHz, pulse duty factor is 0.25, then the cycle is 4 μ s, pulsewidth and shutter
Time is 1 μ s, and incident illumination is different with emergent light position, time delay within 2 μ s, can measure the life-span 1 μ s with
On sample.
According to above-mentioned configuration, it is possible to achieve the measurement of the delayed luminescence of existing most of delayed fluorescence molecules.
In above-mentioned light path, it is also possible to increase lens, make exciting light concentrate on sample, or make sample luminescence focus on
On detector, with enhancing signal intensity.
The method can arrange in pairs or groups arbitrary stable light source use, it is also possible to collocation high power laser, send out for upper conversion
The measurement of the time resolved spectroscopy of light.
Embodiment 2, chopper is for the imaging of delayed luminescence.
As shown in Figure 3, exciting light is changed into light-pulse generator through chopper disk 301 to the structural representation of imaging device, then
Through lens 303, reflect through illuminator 304, then the sample 307 in dichroic mirror 305 reflected illumination sample room 306,
Sample luminescence is according to this through dichroic mirror 305, lens 308, then arrives detector 309 after chopper disk 301.
In chopper disk rotation process, exciting light has to pass through the light hole 302 of chopper disk 301 could irradiate sample, sample
Luminescence also has to pass through light hole 302 and gets to detector, but this twice light is different through the time of light hole, there is elder generation
Rear order, thus detector can shoot the delayed luminescence photo of sample.The principle of delay measurements and embodiment 1 phase
With.
Embodiment 3, chopper is for the micro-imaging of delayed luminescence.
As shown in Figure 4, exciting light passes through chopper disk 401 to microscope structure schematic diagram, passes through after being reflected by illuminator 403 again
Filter 404, then through object lens 406 after dichroic mirror 405 reflects, irradiate the sample 408 on specimen holder 407, sample is luminous
Sequentially pass through object lens 406, dichroic mirror 405, filter 409, again pass by chopper blade 401 and arrive detector.
When above light hole 402 is positioned at illuminator 403, exciting light could pass through, when light hole 402 is positioned at filter 409
During top, sample luminescence gets to detector.Owing to light hole 402 can not be positioned on illuminator 403 and filter 409 simultaneously
Side, when chopper blade 401 rotates, light hole 402 can be alternately located in above the two, and in one-shot measurement, exciting light first passes through
Light hole excites sample, and followed by sample luminescence is detected, and i.e. reaches to excite the purpose postponing imaging afterwards.
In general microscope, the light path design that conventional incident illumination is vertical with emergent light is on this microscope, permissible
Being designed as by chopper blade tapered, as it is shown in figure 5, chopper disk 501 is tapered, upper EDS maps light hole 502, this chopper disk rotates
Time, simultaneously to orthogonal two light path chopping the lights, thus the microscopic imaging device shown in structural map 5 can be can be used to.
Exciting light passes sequentially through chopper disk 501, filter 503, after dichroic mirror 504 reflects, then through object lens 505,
Irradiating the sample 507 on specimen holder 506, sample luminescence sequentially passes through object lens 505, dichroic mirror 504, filter 508, again passes by
Chopper disk 501 arrives detector 509.Similar with the device in Fig. 4, exciting light and sample luminescence are entered by same chopper disk simultaneously
Row chopping the light, thus it postpones the principle of imaging as the device in Fig. 4.
Embodiment 4, chopper is for the inverted microscope of delayed luminescence.
As shown in Figure 4, exciting light passes sequentially through chopper blade 601, filter 603 to microscope structure schematic diagram, irradiates specimen holder
Sample 605 on 604, sample is luminous after object lens 606 below, through the reflection of illuminator 607, then through filter
608, then through lens 610 after being reflected by illuminator 609, again pass by chopper blade 601 and arrive detector 611.
When above light hole 602 is positioned at filter 603, exciting light could pass through, when light hole 602 is positioned on lens 610
Fang Shi, sample luminescence gets to detector.Owing to light hole 602 can not be positioned at above filter 603 and lens 610 simultaneously, when
When chopper blade 601 rotates, light hole 602 can be alternately located in above the two, and in one-shot measurement, exciting light first passes through light hole
Exciting sample, followed by sample luminescence is detected, and i.e. reaches to excite the purpose postponing imaging afterwards.
Relative to the general microscopical same category of device of stable state, the device in example 3 and example 4 only more is loaded with one
Chopper and minority eyeglass, relative to the microscope differentiated At All Other Times, this device need not special light-pulse generator, is also not required to
Want delay controller, ccd detector with gate control function, thus with low cost.The various stable light source furthermore, it is possible to arrange in pairs or groups,
Including xenon lamp mercury lamp LED, high-power near-infrared light source, for the Time-resolved imaging of upper conversion.
Additionally, example 1 is in all devices in example 4, prism can replace mutually with illuminator, can reach similar technology
Effect.The chopper disk of other hole count can be used, to change chopper frequency.Can also be according to the character of measured object luminescence, at light
Grating, lens, optical filter, light splitting piece, attenuator etc. are added, it is also possible to increase dichroic mirror, prism and illuminator to change on road
The direction on darkening road, to strengthen the signal to noise ratio of detection.
Claims (7)
1. the method that chopper is applied to photoluminescence measurement, may be used for the measurement of delayed luminescence, it is characterised in that:
Chopper used uses motor modulation, containing a chopper disk, light hole is distributed on chopper disk；
Having two light paths in measurement: light path one is the light path of excitation light irradiation sample, light path two is that detector detection sample is luminous
Light path；
Utilize illuminator or prism wherein one or two light paths to be reflected or refraction, make two light paths all pass through this and cut
The chopper disk of light device, but the diverse location on chopper disk；
Thus achieve the luminescence the most simultaneously to exciting light and sample of the same chopper and carry out chopping the light, due to
Article two, light path is different through the position of chopper disk, thus twice chopping the light exists time difference, thus this measurement system can detect sample
The delayed luminescence of product and lifetime of excited state.
2. the method for claim 1 is in the measurement system of delayed luminescence, and this system contains light source, specimen holder or sample
Product room, detector, possibly together with a chopper and several prism or illuminator；
It is characterized in that: system exists two light paths: light path one is the light path of excitation light irradiation sample, and light path two is detector
The light path that detection sample is luminous；
Exciting light is irradiated on sample by chopper disk, and sample sends fluorescence or phosphorescence, a portion fluorescence after being stimulated
Or phosphorescence again passes by chopper disk after the reflection of prism or illuminator, is detected by the detector afterwards.
3. the method for claim 1 is in the imaging system of delayed luminescence, it is characterised in that:
Exciting light is changed into light-pulse generator through chopper disk 301, then through lens 303, reflects through illuminator 304, then pass through
Sample 307 in dichroic mirror 305 reflected illumination sample room 306, sample luminescence according to this through dichroic mirror 305, lens 308,
Detector is arrived again after chopper disk 301；
Wherein, exciting light is different by the time of light hole 302 with sample luminescence, there is precedence, thus detector is permissible
The delayed luminescence photo of sample can be shot.
4. the method for claim 1 is for the micro-imaging of delayed luminescence, it is characterised in that:
Exciting light passes through chopper disk 401, again through filter 404 after being reflected by illuminator 403, then reflects through dichroic mirror 405
After through object lens 406, irradiate the sample 408 on specimen holder 407, sample luminescence sequentially passes through object lens 406, dichroic mirror 405, filter
Mirror 409, again passes by chopper blade 401 and arrives detector；
Wherein, exciting light and the luminous light hole 402 that can not simultaneously pass through on chopper disk 401 of sample, there is precedence, thus
Detector can shoot the delayed luminescence photo of sample.
5. the method for claim 1 is for the micro-imaging of delayed luminescence, it is characterised in that:
Exciting light passes sequentially through chopper disk 501, filter 503, after dichroic mirror 504 reflects, then through object lens 505, irradiates
Sample 507 on specimen holder 506, sample luminescence sequentially passes through object lens 505, dichroic mirror 504, filter 508, again passes by chopping the light
Dish 501 arrives detector；
Wherein, chopper disk 501 used is tapered, upper EDS maps light hole 502, when this chopper disk rotates, and can be simultaneously to phase
Two the most vertical light path chopping the lights.
6. the method for claim 1 is in inverted microscope, it is characterised in that:
Exciting light passes sequentially through chopper blade 601, filter 603, irradiates the sample 605 on specimen holder 604, and sample luminescence is under it
After the object lens 606 of side, through the reflection of illuminator 607, then through filter 608, then through lens after being reflected by illuminator 609
610, again pass by chopper blade 601 and arrive detector；
Wherein, can not there is precedence simultaneously by the light hole of chopper disk, thus detector can in exciting light and sample luminescence
So that the delayed luminescence photo of sample can be shot.
7. the method as described in claim 1-6 is used for manufacture and the use of pertinent instruments.
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| CN106483656A (en) * | 2016-12-15 | 2017-03-08 | 武汉能斯特科技有限公司 | A kind of column type optical chopper for delayed luminescence measurement |
| CN106525807A (en) * | 2017-01-20 | 2017-03-22 | 武汉能斯特科技有限公司 | Method and device for measuring fluid delayed luminescence |
| CN108627484A (en) * | 2018-04-26 | 2018-10-09 | 华中科技大学 | A kind of automatic locking phase module and fluorescence lifetime detection method and device |
| CN109827935A (en) * | 2019-02-20 | 2019-05-31 | 南京大学 | A kind of fluorescent microscopic imaging devices and methods therefor using delayed fluorescence |
| CN110146493A (en) * | 2018-02-11 | 2019-08-20 | 博阳生物科技（上海）有限公司 | A kind of light path detection and the light-induced chemiluminescent detector with the system |
| CN111398230A (en) * | 2019-03-27 | 2020-07-10 | 上海交通大学 | Time-gated fluorescence imaging system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN205080051U (en) * | 2015-10-07 | 2016-03-09 | 朱泽策 | Can be applied to transient state spectrum appearance and time -resolved image device's photointerrupter |
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| CN205080051U (en) * | 2015-10-07 | 2016-03-09 | 朱泽策 | Can be applied to transient state spectrum appearance and time -resolved image device's photointerrupter |
| CN105527265A (en) * | 2016-01-22 | 2016-04-27 | 复旦大学 | Laser pump time resolution up-conversion luminescence living imaging system |
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| CN106483656A (en) * | 2016-12-15 | 2017-03-08 | 武汉能斯特科技有限公司 | A kind of column type optical chopper for delayed luminescence measurement |
| CN106525807A (en) * | 2017-01-20 | 2017-03-22 | 武汉能斯特科技有限公司 | Method and device for measuring fluid delayed luminescence |
| CN110146493A (en) * | 2018-02-11 | 2019-08-20 | 博阳生物科技（上海）有限公司 | A kind of light path detection and the light-induced chemiluminescent detector with the system |
| CN108627484A (en) * | 2018-04-26 | 2018-10-09 | 华中科技大学 | A kind of automatic locking phase module and fluorescence lifetime detection method and device |
| CN108627484B (en) * | 2018-04-26 | 2020-05-19 | 华中科技大学 | Automatic phase-locking module and fluorescence life detection method and device |
| CN109827935A (en) * | 2019-02-20 | 2019-05-31 | 南京大学 | A kind of fluorescent microscopic imaging devices and methods therefor using delayed fluorescence |
| CN109827935B (en) * | 2019-02-20 | 2020-05-01 | 南京大学 | Fluorescence microscopic imaging device and method using delayed fluorescence |
| CN111398230A (en) * | 2019-03-27 | 2020-07-10 | 上海交通大学 | Time-gated fluorescence imaging system |
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