CN202149881U - Time-resolved photo-induced organism ultra-weak photon detection system - Google Patents
Time-resolved photo-induced organism ultra-weak photon detection system Download PDFInfo
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- CN202149881U CN202149881U CN201120169134U CN201120169134U CN202149881U CN 202149881 U CN202149881 U CN 202149881U CN 201120169134 U CN201120169134 U CN 201120169134U CN 201120169134 U CN201120169134 U CN 201120169134U CN 202149881 U CN202149881 U CN 202149881U
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Abstract
The utility model discloses a time-resolved photo-induced organism ultra-weak photon detection system. The system consists of a light source, an optical module and a photon counting and time sequence control system, wherein the light source is coupled to an incident shutter of the optical module through an optical fiber; the module is a double-confocal light path system consisting of two electronic light shutters, two collimating lenses, a band-pass and long-pass filter, a spectrophotometric two-color mirror and an objective lens; and after induced light is collimated, the induced light is reflected to the objective lens through the spectrophotometric two-color mirror and is focalized to a measured sample. Ultra-weak light produced by the sample is acquired by the same objective lens, is returned along an original path, enters the other light path which is conjugated with the spectrophotometric two-color mirror, and is coupled to a photomultiplier by a lens after passing through an exit shutter. The output signal of the photomultiplier enters a multi-channel photon counter and a data processor after passing through preamplifier. All measurement is program-controlled by a time sequence circuit. The system can perform sensitive, rapid and nondestructive examination on organisms and pollutants thereof, and is widely applied to the fields such as food security, crop product quality evaluation and monitoring and the like.
Description
Technical field
The utility model belongs to the optical engineering technical field; Be specifically related to a kind of ultraweak photon detection of high-sensitive biosome system to portable development, with this system can to biosome and attached on the biosome/material composition of interior other molecular level carries out sensitivity fast and Non-Destructive Testing.For example applicable to the pesticide concentration in fruit, the vegetables; The content of antibiotic in the aquatic products; Non-Destructive Testing or monitoring (comprising that import, outlet flow to) occasion is carried out in the evaluation of food security such as the adjuvant in granulating grain and the powdery grain and biosome characteristic, the quality of agricultural byproducts and the evaluation of seed quality etc.
Background technology
The content that food safety detection contains is extensive, mainly comprises the analysis etc. of general constituent analysis, trace element analysis, pesticide residue analysis, residue of veterinary drug analysis, mycotoxin analysis, food additives analysis and other objectionable impuritiess of food.Main at present physics and chemistry technological (like AAS, atomic fluorescence spectrometry, electrochemical process, atomic absorption spectrography (AAS), vapor-phase chromatography, high performance liquid chromatography etc.) and the biology techniques of adopting carries out Food Inspection.Wherein most of detection need be carried out in the laboratory, and operating process is complicated, inspection cost is expensive, length, some testing processes consuming time also can damage sample.Therefore; Instrument portability, detection scene and non-destructive analysis have become the direction of current Food Inspection development; Carrying out food security Non-Destructive Testing or the monitoring occasion of (comprising that import, outlet flow to) especially, as: the pesticide concentration in fruit, the vegetables, the content of antibiotic in the aquatic products; Adjuvant in granulating grain and the powdery grain or the like needs quick, harmless, portable detecting instrument device.In addition, need develop simple and effective analytical approach and technology for the quality of agricultural byproducts and the evaluation of seed quality.
Summary of the invention
Whether the purpose of the utility model is in the food security field biosome self property to be changed and receive and polluting and food quality problems such as pollution level are carried out easily and fast, Non-Destructive Testing; The ultraweak photon detection of a kind of time-resolved photoinduction biosome system is provided, can becomes the useful of existing detection technique and replenish.
The superweak photon radiation of photoinduction is the intrinsic phenomenon of biosome, when the character of biosome self changes, or receives the influence (like agricultural chemicals, adjuvant etc.) of extraneous factor, and the superweak characteristics of luminescence of the photoinduction of biosome can change.Therefore, the superweak photon radiation of photoinduction can reflect the Global Information of biosome by interior change and the caused biological effect of environmental impact, and an overall target about the biosystem fundamental characteristics is provided.Use the utility model system and device to realize quick, Non-Destructive Testing through the superweak characteristics of luminescence of the photoinduction of biosome intrinsic to food quality.In addition, native system adopts Optical Fiber Transmission and conjugated optical channels design, has simplified optical system, can realize miniaturization and portable, is applicable to the superweak luminous measurement of photoinduction biosome of multiple occasion and various sample forms.
The ultraweak photon detection of the time-resolved photoinduction biosome system that the utility model provides comprises:
Wideband light source or narrow-band light source, wideband light source comprise xenon lamp, deuterium lamp and xenon flash lamp, and narrow-band light source comprises laser, semiconductor laser and LED, and wideband light source or narrow-band light source are connected to incident end electronic light shutter through coupled fiber or coupled lens;
Incident end electronic light shutter connects the first optical alignment lens, bandpass filter and beam split dichroscope successively;
The beam split dichroscope is divided into two-way, and one the tunnel connects object lens, and through object lens will from said light source as the luminous irradiates light of photoinduction biosome or induce illumination to be mapped on the test sample in the sample chamber; Dichroiscopic another road of beam split connects successively to grow logical or be with and leads to or narrow band pass filter, the second optical alignment lens and exit end electronic light shutter; The beam split dichroscope connects simultaneously and is used to eliminate parasitic light to reduce the attenuator of interference of stray light;
Exit end electronic light shutter connects collimation or imaging len and photomultiplier successively, and photomultiplier connects the preposition electron-amplifier in broadband, multi-channel photon counter and data processor successively;
Exit end electronic light shutter, multi-channel photon counter, incident end electronic light shutter and wideband light source or narrow-band light source are connected with time schedule controller simultaneously.Time schedule controller sends four tunnel programme controlled level signals, triggers the opening and closing of light source and multi-channel photon counter, two electronic light shutters of control respectively.
Described incident end electronic light shutter, the first optical alignment lens, bandpass filter, beam split dichroscope, object lens, attenuator, length are led to or band is logical or narrow band pass filter, the second optical alignment lens, collimation or imaging len, exit end electronic light shutter and sample chamber adopt optical module design to process an integral body.
The course of work of the utility model
From above-mentioned wideband light source or narrow-band light source as the luminous irradiates light of photoinduction biosome or induce light; Be coupled to the Guang Lan center (calling " incident shutter " in the following text) of the incident end electronic light shutter of said optical system; Behind the incident shutter opening; Arrive beam split dichroscope (short anti-length is logical, or opposite) through first optical alignment lens combination and the bandpass filter that is installed in thereafter successively.The parasitic light that sees through beam split dichroscope (short anti-long logical type) gets into and is positioned at attenuator thereafter, to reduce interference of stray light; The object lens with different multiples by the light of most of useful wave band of this beam split dichroscope reflection is formed via lens combination focus on it on sample, behind the incident light shutter close, and completion photoinduction effect.The photoinduction superweak luminous (calling " superweak photon " in the following text) that sample produces is gathered by same object lens; And get into same beam split dichroscope with directional light mode reverse transfer once more; Its permeation parts gets into length logical (or band the is logical or the arrowband) optical filter that vertical objective lens optical axis is installed then; Superweak photon beam after the optical filtering is positioned at the Guang Lan center that thereafter second optical alignment lens combination focuses on exit end electronic light shutter (calling " outgoing shutter " in the following text), and the Guang Lan center of two electronic light shutters is in mutual conjugate position with respect to the beam split dichroscope.After this shutter was opened, the superweak photon beam of outgoing was collimated on the photocathode of photomultiplier by convex lens thereafter, accomplished the photosignal conversion.The photomultiplier output terminal is connected to the preposition electron-amplifier in broadband, and this prime amplifier and multi-channel photon counter and data processor have been formed high-sensitive time discrimination measurement and register system.
Advantage of the utility model and good effect
Advantages such as the retardation time that the utility model system has is highly sensitive, dark noise is little, measure delayed luminescence (inducing light with respect to closing) is short, application flexibility is high, extendability is strong; Can realize biosome and carry out quick nondestructive attached to the material composition of other molecular level of biosome and detect; Be applicable to the pesticide concentration in fruit, the vegetables; The content of antibiotic in the aquatic products; Three chlorocyanamides in the milk, the adjuvants in granulating grain and the powdery grain etc. need carry out the occasion of Non-Destructive Testing or monitoring (comprising that such biosome import of goods, such biosome export of goods flow to) to food security; The method for quick that also can be used for development evaluation agricultural quality and seed quality.
Description of drawings
Fig. 1 is the ultraweak photon detection systematic schematic diagram of time-resolved photoinduction biosome.
Fig. 2 obtains time-resolved photon steradiance change curve.
Among the figure, 101 is broadband or narrow-band light source; 201 are incident end electronic light shutter; 301 is the first optical alignment lens; 401 is bandpass filter; 501 are the beam split dichroscope; 601 is object lens; 701 is attenuator; 801 are long logical (or band is logical or the arrowband) optical filter; 901 is the second optical alignment lens; 902 is collimation or imaging len; 1001 is exit end electronic light shutter; 1002 are photomultiplier (comprising refrigeration and high pressure); 1003 is the preposition electron-amplifier in broadband; 1004 is the multi-channel photon counter; 1005 is data processor; 1006 is time schedule controller; 100 is the sample chamber.
The optical module design comprises following optical element: 201 incident end electronic light shutters, 301 first optical alignment lens, 401 bandpass filters; 501 beam split dichroscopes, 601 object lens, 701 attenuators; 801 long logical (or band is logical or the arrowband) optical filter; 901 second optical alignment lens, 902 collimations or imaging len, 1001 is exit end electronic light shutter and 100 sample chambers.
The integral module design comprises: optical module, 101 light sources and 1002 photomultipliers.
Embodiment
Embodiment 1
As shown in Figure 1, the concrete formation of the utility model system is:
Flash of light xenon lamp 101 through being directed into incident end electronic light shutter 201 with the multimode optical fiber coupling, becomes parallel beam as the photoinduction light source behind the first optical alignment lens 301.(transmitance of cutoff wavelength part is 10 to select the light conduct of ultraviolet band to induce light through bandpass filter 401
-5), directional light focuses on sample 100 through object lens 601 after 501 reflections of beam split dichroscope.Simultaneously, visible light and short wave ultraviolet light composition residual after bandpass filter 401 filters get into delustring device 701 through beam split dichroscope 501, avoid its scattering in device that the measurement of weak signal light is exerted an influence.
The superweak photo emissions that sample receives to produce after the illumination is gathered through object lens 601, and (transmitance to irradiation optical wavelength part is 10 through beam split dichroscope 501, long pass filter 801 successively with the directional light form
-5) after, converge at the smallcolumn diaphragm center of exit end electronic light shutter 1001 by the second optical alignment lens 901, get into photomultipliers 1002 through collimation lens 902 then.
Photomultiplier 1002 is an electric impulse signal with superweak photon conversion, and input multi-channel photon counter 1004 obtains time-resolved photon steradiance change curve after preposition electron-amplifier 1003 amplifies, and is as shown in Figure 2.At last this curve is imported data processor 1005 and carry out data analysis.
The operational process of the utility model system is following:
Under the original state, incident shutter 201 is closed with outgoing shutter 1001, and light source 101 is in holding state with multi-channel photon counter 1004.
During measurement, at first open incident shutter 201, after shutter is opened fully and is stable, trigger flashing xenon lamp 101, light is induced in output to make it (preestablish irradiates light power and irradiation time) in the intended manner.When illumination finishes, close incident shutter 201 to eliminate the influence of exciting light twilight sunset and the outer parasitic light of device.When closing incident shutter 201, to open outgoing shutter 1001 and trigger multi-channel photon counter 1004, the faint photon that sample is sent is collected in time and is measured.After measuring end, close outgoing shutter 1001, system and device is returned to original state, measures the repetition said process next time.Accurate, the synthetic operation under the level signal control of time schedule controller 1006 of total system device.
The superweak photon steradiance of photoinduction biosome that multi-channel photon counter 1004 measures die-away curve in time is imported into data processor 1005 dynamic analysis that decays; Obtain corresponding decay of luminescence dynamic characteristic parameter (luminescent lifetime T, luminous initial strength I
0And total photon number N).According to T, I
0, the N value, draw respective detection or monitoring conclusion value D through the data storage matrix table.
Utility model system applies example
Below in conjunction with the practical application example, further specify the utility model system, said example does not influence the further protection of the utility model.
For example, randomly draw the residual quantity that a fruit detects its agricultural chemicals.Fruit is placed on 100 position.
Under the control of time schedule controller 1006, light source 101 sends induces light successively through incident shutter 201, the first optical alignment lens 301, bandpass filter 401, after 501 reflections of beam split dichroscope, focuses on tested fruit 100 by object lens 601 again;
Tested fruit 100 is after irradiation, and under the control of time schedule controller 1006, incident shutter 201 is closed, and the shutter of outgoing simultaneously 1001 is opened.Tested fruit places in the sample chamber 100; Successively through beam split dichroscope 501, long pass filter 801, the second optical alignment lens 901, outgoing shutter 1001, collimation lens 902 then were transformed into electric impulse signal by photomultiplier 1002 after the faint photo emissions that receives to produce after the photoinduction was gathered by object lens 601.This electric impulse signal obtains time-resolved photon steradiance change curve through preposition electron-amplifier 1003 and multi-channel photon counter 1004.
Experimental data is imported into data processor 1005 dynamic analysis that decays, and obtains corresponding decay of luminescence dynamic characteristic parameter (luminescent lifetime T, luminous initial strength I
0And total photon number N).According to T, I
0, N value and the persticide residue and the corresponding relations between these decay of luminescence kinetic parameters set up in advance through a large amount of experiments, draw this fruit respective detection or monitor persticide residue conclusion value D.
Claims (2)
1. the ultraweak photon detection of time-resolved photoinduction biosome system is characterized in that this system comprises:
Wideband light source or narrow-band light source, wideband light source comprise xenon lamp, deuterium lamp and xenon flash lamp, and narrow-band light source comprises laser, semiconductor laser and LED, and wideband light source or narrow-band light source are connected to incident end electronic light shutter through coupled fiber or coupled lens;
Incident end electronic light shutter connects the first optical alignment lens, bandpass filter and beam split dichroscope successively;
The beam split dichroscope is divided into two-way, and one the tunnel connects object lens, and through object lens will from said light source as the luminous irradiates light of photoinduction biosome or induce illumination to be mapped on the test sample in the sample chamber; Dichroiscopic another road of beam split connects successively to grow logical or be with and leads to or narrow band pass filter, the second optical alignment lens and exit end electronic light shutter; The beam split dichroscope connects simultaneously and is used to eliminate parasitic light to reduce the attenuator of interference of stray light;
Exit end electronic light shutter connects collimation or imaging len and photomultiplier successively, and photomultiplier connects the preposition electron-amplifier in broadband, multi-channel photon counter and data processor successively;
Exit end electronic light shutter, multi-channel photon counter, incident end electronic light shutter and wideband light source or narrow-band light source are connected with time schedule controller simultaneously.
2. detection system according to claim 1 is characterized in that described incident end electronic light shutter, the first optical alignment lens, bandpass filter, beam split dichroscope, object lens, attenuator, length are led to or band is logical or narrow band pass filter, the second optical alignment lens, collimation or imaging len, exit end electronic light shutter and sample chamber adopt optical module design to process an integral body.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590171A (en) * | 2012-03-02 | 2012-07-18 | 南开大学 | System for rapidly detecting ocean oil pollution |
| CN103536277A (en) * | 2013-10-30 | 2014-01-29 | 中南民族大学 | Automated ultra-weak light imaging system and operation method |
| CN104062004A (en) * | 2014-06-13 | 2014-09-24 | 中国科学院西安光学精密机械研究所 | Extremely-weak light signal detection device and method |
| CN109738410A (en) * | 2019-01-29 | 2019-05-10 | 依利特(苏州)分析仪器有限公司 | It is a kind of for detecting the automation equipment and method of mycotoxin |
| CN110376937A (en) * | 2019-06-26 | 2019-10-25 | 中国船舶重工集团公司第七一九研究所 | A kind of time sequence control logic and signal processing algorithm suitable for releasing the quasi real time Dose rate measurement device of light technology based on pulsed light |
| CN111157494A (en) * | 2019-12-24 | 2020-05-15 | 新绎健康科技有限公司 | Method and system for controlling and analyzing delayed luminescence |
| CN114740517A (en) * | 2022-03-22 | 2022-07-12 | 国家电网有限公司 | Nondestructive testing system and method for nuclear power station environmental radiation |
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2011
- 2011-05-25 CN CN201120169134U patent/CN202149881U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590171A (en) * | 2012-03-02 | 2012-07-18 | 南开大学 | System for rapidly detecting ocean oil pollution |
| CN103536277A (en) * | 2013-10-30 | 2014-01-29 | 中南民族大学 | Automated ultra-weak light imaging system and operation method |
| CN104062004A (en) * | 2014-06-13 | 2014-09-24 | 中国科学院西安光学精密机械研究所 | Extremely-weak light signal detection device and method |
| CN109738410A (en) * | 2019-01-29 | 2019-05-10 | 依利特(苏州)分析仪器有限公司 | It is a kind of for detecting the automation equipment and method of mycotoxin |
| CN109738410B (en) * | 2019-01-29 | 2021-11-12 | 依利特(苏州)分析仪器有限公司 | Automated device and method for detecting mycotoxins |
| CN110376937A (en) * | 2019-06-26 | 2019-10-25 | 中国船舶重工集团公司第七一九研究所 | A kind of time sequence control logic and signal processing algorithm suitable for releasing the quasi real time Dose rate measurement device of light technology based on pulsed light |
| CN111157494A (en) * | 2019-12-24 | 2020-05-15 | 新绎健康科技有限公司 | Method and system for controlling and analyzing delayed luminescence |
| CN114740517A (en) * | 2022-03-22 | 2022-07-12 | 国家电网有限公司 | Nondestructive testing system and method for nuclear power station environmental radiation |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120222 Termination date: 20140525 |