CN114965400B - Atmospheric microorganism on-line monitor - Google Patents
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Abstract
The invention provides an atmospheric microorganism on-line monitor, which comprises a gas collecting system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system, wherein the gas collecting system is connected with the multispectral detection system; the gas collecting system is used for collecting atmospheric microorganisms in real time; the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals; the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals; the high-speed data acquisition system is used for carrying out analysis inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram, and generating a data report. The invention realizes real-time monitoring of the variety and concentration change of the atmospheric microorganisms, timely performs inversion and result presentation, and timely performs early warning analysis on the abnormal atmospheric microorganisms.
Description
Technical Field
The invention belongs to the technical field of atmospheric environment monitoring, and particularly relates to an atmospheric microorganism on-line monitor.
Background
The atmosphere is an important environment for human life and is closely related to human production and life. But is especially necessary for the control of the atmospheric composition and concentration variation and the guidance and the daily life of people. And atmospheric microorganisms change at time with changes in the atmosphere. The control of the type, content and change rule of microorganisms in the atmosphere is one of the important problems in the world today. Especially in epidemic situation, the real-time on-line monitoring of the microorganisms such as harmful bacteria and viruses in the atmosphere is very important and necessary.
At present, the instruments for monitoring cloud, sand dust, haze, water vapor, nitrogen, ozone and the like in the atmosphere are more mature, and basically realize real-time online monitoring. For the monitoring of atmospheric microorganisms, the detection is basically performed off-line, i.e. the air sample is collected by using a liquid or solid sampler, and then identified, identified or detected by various means in a laboratory according to the need or purpose. The method has the problems of low timeliness, high cost, complex operation and the like, and a large amount of manpower, material resources and financial resources are required to be input. In recent years, a fluorescent laser radar is widely used as an atmospheric microorganism online monitoring instrument, but the fluorescent laser radar is influenced by a detection blind area and cannot detect the earth surface atmosphere or an indoor environment which is close to the work and the life of people and is just in the blind area range, so that monitored data cannot reflect the atmospheric microorganism change of the life of people reliably, and the fluorescent laser radar needs a high-energy laser as a light source, so that the instrument needs a relatively harsh environment to observe. In particular, fluorescent lidars often emit only 1-2 excitation ultraviolet lasers, or only detect a certain band or range of continuous fluorescence spectra, and cannot obtain a full excitation/emission fluorescence spectrum (EEM), resulting in interference or influence of fluorescence signals of other substances when identifying/identifying atmospheric microorganisms.
Disclosure of Invention
In order to solve the technical problems, the invention provides an atmospheric microorganism online monitor which is used for monitoring the types and concentration changes of atmospheric microorganisms in real time, carrying out inversion and result presentation in time and carrying out early warning analysis on the abnormal atmospheric microorganisms in time.
The invention provides an atmospheric microorganism on-line monitor for achieving the aim, which comprises a gas collecting system, a multispectral detection system, a fluorescence service life detection system and a high-speed data acquisition system;
the gas collecting system is used for collecting atmospheric microorganisms in real time;
the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals;
the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals;
the high-speed data acquisition system is used for carrying out analysis inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram, and generating a data report.
Optionally, the gas collecting system includes: the device comprises a coarse filter screen, a drainage tube, a gas collecting tube, a pump suction tube and a circulating pump;
the rough filter screen is connected with an air inlet of the gas collecting tube through the drainage tube, and an air outlet of the gas collecting tube is connected with the circulating pump through the pump suction tube.
Optionally, the multispectral detection system includes: the high-energy ultraviolet xenon lamp, a 15-bit narrow-band optical filter rotating wheel assembly, a first collecting lens, a first plano-convex lens, a 15-bit chopping optical filter rotating wheel assembly, a second planoconvex lens, an optical fiber connecting plate, multimode optical fibers and a multispectral detector;
the high-energy ultraviolet xenon lamp and the 15-bit narrow-band filter rotating wheel assembly are positioned on one side of the gas collecting tube, and the 15-bit narrow-band filter rotating wheel assembly is positioned on the optical axis of the high-energy ultraviolet xenon lamp;
the first condenser, the first plano-convex lens, the 15-bit chopping filtering rotating wheel assembly, the second plano-convex lens and the optical fiber connecting plate are coaxially positioned on the other side of the gas collecting tube in sequence and positioned in the non-coaxial direction of the high-energy ultraviolet xenon lamp;
the optical fiber connecting plate is connected with the multispectral detector through the multimode optical fiber.
Optionally, the optical axes of the first condenser and the first plano-convex lens are in the same plane with the optical axis of the high-energy ultraviolet xenon lamp and are perpendicular to the central line of the gas collecting tube.
Optionally, the fluorescence lifetime detection system comprises: the high-frequency pulse laser, the high-energy optical filter, the third flat convex lens, the second condenser, the fourth flat convex lens, the long wave transmission optical filter, the fifth flat convex lens and the photomultiplier;
the high-frequency pulse laser, the high-energy optical filter and the third plano-convex lens are positioned on one side of the gas collecting tube;
the high-energy optical filter and the third plano-convex lens are positioned in the transmitting direction of the transmitting optical axis of the high-frequency pulse laser, and the third plano-convex lens is focused at the center line position of the gas collecting tube.
The second condenser, the fourth plano-convex mirror, the long wave transmitting optical filter, the fifth plano-convex mirror and the photomultiplier are coaxially positioned on the other side of the gas collecting tube in sequence and positioned in the non-coaxial direction of the transmitting optical axis of the high-frequency pulse laser;
the axis of the second condenser is in the same plane with the axis of the high-frequency pulse laser and perpendicularly intersects with the central line of the gas collecting tube.
Optionally, the high-frequency pulse laser, the high-energy optical filter, the third plano-convex mirror, the high-energy ultraviolet xenon lamp and the 15-bit narrow-band optical filter rotating wheel assembly are located on the same side of the gas collecting tube and are arranged in a layered mode.
Optionally, the high-speed data acquisition system includes: the device comprises a high-energy ultraviolet xenon lamp power supply, a high-frequency pulse laser power supply, a filter disc rotating wheel driver, a time-dependent single photon counter, an embedded industrial personal computer and on-line monitoring software;
the high-energy ultraviolet xenon lamp power supply is connected with the high-energy ultraviolet xenon lamp, the filter disc rotating wheel driver is respectively connected with the 15-bit narrow-band filter rotating wheel assembly and the 15-bit chopping filter rotating wheel assembly, the high-frequency pulse laser power supply is connected with the high-frequency pulse laser, the time-dependent single photon counter is respectively connected with the high-frequency pulse laser power supply and the photomultiplier, the embedded industrial personal computer is respectively connected with the high-energy ultraviolet xenon lamp power supply, the high-frequency pulse laser power supply, the filter disc rotating wheel driver, the time-dependent single photon counter and the circulating pump, and the on-line monitoring software is located on the embedded industrial personal computer.
Optionally, a total of 15 narrowband filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially installed on the 15-bit narrowband filter runner assembly in a counterclockwise direction;
and a total of 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering rotating wheel assembly in a anticlockwise manner.
Optionally, the narrowband filter on the 15-bit narrowband filter runner assembly and the long-wave pass filter on the 15-bit chopper filter runner assembly sequentially correspond to each other according to a counterclockwise installation sequence;
the on-line monitoring software controls the filter disc rotating wheel driver to drive the 15-bit narrow-band filter rotating wheel assembly to rotate clockwise through the embedded industrial personal computer, and synchronously drives the 15-bit chopping filtering rotating wheel assembly to rotate clockwise.
Compared with the prior art, the invention has the following advantages and technical effects:
the invention provides an on-line monitor specially aiming at atmospheric microorganisms, which is characterized in that the atmospheric microorganisms are input into an instrument in real time through an input detection method, the input atmospheric microorganisms are respectively excited by ultraviolet light in a plurality of wave bands, fluorescence spectrum signals are obtained, the collected fluorescence spectrum signals are further analyzed and processed to form a multi-wavelength excitation-fluorescence spectrum (EEM) and fluorescence service life, so that the identification and concentration measurement of microorganisms such as bacteria and viruses in the atmosphere are realized, a data report is generated, and timely early warning is carried out according to the types and concentration changes of the atmospheric microorganisms in the data report, so that measures are taken to timely kill harmful microorganisms and protect the harmful microorganisms. Meanwhile, the real-time online sampling also avoids the injury of harmful microorganisms to sampling personnel.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application, illustrate and explain the application and are not to be construed as limiting the application. In the drawings:
FIG. 1 is a schematic diagram of an atmospheric microorganism on-line monitor according to an embodiment of the present invention;
wherein: 1. a coarse filter screen; 2. a drainage tube; 3. a gas collecting tube; 4. a pump suction pipe; 5. a circulation pump; 6. a high energy ultraviolet xenon lamp power supply; 7. a high energy ultraviolet xenon lamp; 8. a filter wheel drive; 9. 15 bit narrow band filter runner components; 10. a first condenser; 11. a first plano-convex mirror; 12. 15-bit chopping and filtering rotating wheel assembly; 13. a second plano-convex mirror; 14. an optical fiber connection board; 15. a multimode optical fiber; 16. a multispectral detector; 17. a high frequency pulsed laser power supply; 18. A high frequency pulse laser; 20. a high-energy optical filter; 21. a third plano-convex mirror; 22. a second condenser lens; 23. a fourth plano-convex mirror; 24. a long wave transmitting filter; 25. a fifth plano-convex mirror; 26. a photomultiplier tube; 27. a time-dependent single photon counter; 28. an embedded industrial personal computer; 29. and (5) online monitoring software.
Detailed Description
It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other. The present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
It should be noted that the steps illustrated in the flowcharts of the figures may be performed in a computer system such as a set of computer executable instructions, and that although a logical order is illustrated in the flowcharts, in some cases the steps illustrated or described may be performed in an order other than that illustrated herein.
Examples
As shown in FIG. 1, the embodiment provides an atmospheric microorganism on-line monitor, which comprises a gas collecting system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system; the gas collecting system is used for collecting atmospheric microorganisms in real time; the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals; the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals; the high-speed data acquisition system is used for carrying out analysis inversion according to the fluorescence spectrum signal data and the fluorescence lifetime signal data to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram, and generating a data report.
Wherein, the gas collecting system includes: the device comprises a coarse filter screen 1, a drainage tube 2, a gas collecting tube 3, a pump suction tube 4 and a circulating pump 5;
in this embodiment, the coarse screen 1 is connected to the air inlet of the air collecting pipe 3 through the drainage pipe 2, and the air outlet of the air collecting pipe 3 is connected to the circulation pump 5 through the pump suction pipe 4.
The coarse filter screen 1 blocks large-particle impurities, flies and the like from entering the system, so that the blockage of a gas collecting system is prevented, and the drainage tube 2 introduces external atmosphere into the gas collecting tube 3; the gas collecting tube 3 is used as an important online structure, and a device for preventing microorganisms from flowing through rapidly is arranged inside the gas collecting tube 3, so that microorganisms with sufficient concentration are always ensured to gather in the gas collecting tube 3 for monitoring. The circulating pump 5 provides transmission power for the whole gas collecting system through the pump suction pipe 4.
The multispectral detection system includes: the high-energy ultraviolet xenon lamp 7, the 15-bit narrow-band filter runner assembly 9, the first condenser lens 10, the first plano-convex lens 11, the 15-bit chopping filter runner assembly 12, the second plano-convex lens 13, the optical fiber connecting plate 14, the multimode optical fiber 15 and the multispectral detector 16;
the high-energy ultraviolet xenon lamp 7 and the 15-bit narrow-band filter rotating wheel assembly 9 are positioned on one side of the gas collecting tube 3, and the 15-bit narrow-band filter rotating wheel assembly 9 is positioned on the optical axis of the high-energy ultraviolet xenon lamp 7, is positioned on the same plane with the optical axis of the high-energy ultraviolet xenon lamp 7 and is perpendicular to the central line of the gas collecting tube 3; the first condenser lens 10, the first plano-convex lens 11, the 15-bit chopping filtering rotating wheel assembly 12, the second plano-convex lens 13 and the optical fiber connecting plate 14 are sequentially and coaxially positioned on the other side of the gas collecting tube 3 and positioned in the non-coaxial direction of the high-energy ultraviolet xenon lamp 7; the optical fiber connection plate 14 is connected with the multispectral detector 16 through the multimode optical fiber 15.
In this embodiment, the high-energy ultraviolet xenon lamp 7 is focused on the middle line of the gas collecting tube 3, and the 15-bit narrow-band filter runner assembly 9 is installed on the optical axis of the high-energy ultraviolet xenon lamp 7 between the high-energy ultraviolet xenon lamp 7 and the gas collecting tube 3. The first condenser 10, the first plano-convex mirror 11, the 15-bit chopping filtering rotating wheel assembly 12, the second plano-convex mirror 13 and the optical fiber connecting plate 14 are coaxially arranged in sequence on the other side of the gas collecting tube 3 and in the direction of being non-coaxial with the optical axis of the high-energy ultraviolet xenon lamp 7, the optical fiber connecting plate 14 is connected with the multispectral detector 16 through the multimode optical fiber 15, and the first plano-convex mirror is perpendicular to the central line of the gas collecting tube 3 and is in the same plane with the optical axis of the high-energy ultraviolet xenon lamp 7. Wherein, 15 narrow-band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially arranged on the 15-bit narrow-band filter runner assembly 9 in a anticlockwise manner; 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering runner assembly 12 in a anticlockwise manner.
The high-energy ultraviolet xenon lamp 7 is used as an important excitation light source of the multispectral detection system, so that the output light beam of the high-energy ultraviolet xenon lamp 7 has higher energy distribution in an ultraviolet band, the utilization efficiency of the xenon lamp is greatly improved by adopting the xenon lamp with internal arc reflection, the light beam output by the high-energy ultraviolet xenon lamp 7 is directly focused to one point, and the filter is arranged in front of the focal point of the light beam, so that the volume of an instrument is greatly reduced, and the space utilization rate is improved. 15 narrow-band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially arranged on the 15-bit narrow-band filter runner assembly 9 in a anticlockwise manner, and 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopper filter runner assembly 12 in a anticlockwise manner to synchronously operate, so that one-to-one correspondence of excitation light and excitation obtained fluorescence spectrum signals is ensured. The use of the first condenser lens 10 ensures that a sufficient number of fluorescent signals are received and the use of the second plano-convex lens 13, the fiber optic connection plate 14 and the multimode optical fiber 15 allows the system to be made more compact. The multispectral detector 16 is used as an important acquisition system of the multispectral detection system, and a multi-wavelength fluorescence spectrum acquisition device, such as a P32 multispectral laser radar detector of the licel company of Germany, is adopted to better realize detection of wider fluorescence spectrum signals. Meanwhile, the first collecting lens 10 and the optical axis of the high-energy ultraviolet xenon lamp 7 are arranged on the same plane, so that the first collecting lens 10 can obtain more strong fluorescence spectrum signals, and the signal quality is greatly improved; the first condensing lens 10 and the high-energy ultraviolet xenon lamp 7 are arranged on the side face of the gas collecting tube 3 in a non-coaxial mode, damage of strong light irradiation to the multispectral detector 16 and the like in the coaxial mode is avoided, and the service life of the instrument is greatly prolonged.
The fluorescence lifetime detection system comprises: a high-frequency pulse laser 18, a high-energy filter 20, a third plano-convex mirror 21, a second condenser 22, a fourth plano-convex mirror 23, a long-wave-transmitting filter 24, a fifth plano-convex mirror 25, and a photomultiplier 26;
the high-frequency pulse laser 18, the high-energy optical filter 20 and the third plano-convex mirror 21 are positioned on one side of the gas collecting tube 3; the high-energy optical filter 20 and the third plano-convex mirror 21 are positioned in the emission direction of the emission optical axis of the high-frequency pulse laser 18, and the third plano-convex mirror 21 is focused on the center line position of the gas collecting tube 3 and is positioned on the same plane with the emission optical axis of the high-frequency pulse laser 18; the second condenser 22, the fourth plano-convex lens 23, the long-wave transmission filter 24, the fifth plano-convex lens 25 and the photomultiplier 26 are coaxially positioned on the other side of the gas collecting tube 3 in sequence and positioned in a non-coaxial direction of the emission optical axis of the high-frequency pulse laser 18; the axis of the second condenser 22 perpendicularly intersects with the center line of the header 3.
The high-frequency pulse laser 18, the high-energy optical filter 20 and the third plano-convex mirror 21 and the high-energy ultraviolet xenon lamp 7 and the 15-bit narrow-band optical filter runner assembly 9 are positioned on the same side of the gas collecting tube 3 and are arranged in a layered mode.
In the present embodiment, the high-energy optical filter 20 and the third plano-convex mirror 21 are sequentially arranged in the emission direction of the emission optical axis of the high-frequency pulse laser 18, and the third plano-convex mirror 21 is focused at the center line position of the gas collecting tube 3; the second condenser 22, the fourth plano-convex mirror 23, the long wave transmitting filter 24, the fifth plano-convex mirror 25 and the photomultiplier 26 are coaxially arranged in sequence on the other side of the gas collecting tube 3 in a direction which is not coaxial with the optical axis of the high-frequency pulse laser 18 and is perpendicular to the central line of the gas collecting tube 3 and is in the same plane with the optical axis of the high-frequency pulse laser 18, and the axis of the second condenser 22 intersects with the central line of the gas collecting tube 3. The fluorescence lifetime detection system is provided with an expansion port, and can be expanded into multiple paths of fluorescence lifetime to be detected simultaneously according to the needs. The high frequency pulse laser 18 employs a high repetition rate femtosecond or picosecond laser, greatly improving the accuracy of fluorescence lifetime detection. Photomultiplier tube 26 may employ PMT or APD with built-in amplifier and temperature control, greatly improving detection accuracy and sensitivity. Meanwhile, the second condenser lens 22 and the optical axis of the high-frequency pulse laser 18 are arranged on the same plane, so that the second condenser lens 22 can obtain more strong fluorescence lifetime signals, and the signal quality is greatly improved; the second condenser 22 and the high-frequency pulse laser 18 are arranged on the side face of the gas collecting tube 3 in a non-coaxial mode, damage of strong light irradiation to the photomultiplier 26 and the like in the coaxial mode is avoided, and the service life of the instrument is greatly prolonged.
The high-speed data acquisition system includes: the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter disc rotating wheel driver 8, the time-related single photon counter 27, the embedded industrial personal computer 28 and the on-line monitoring software 29;
the high-energy ultraviolet xenon lamp power supply 6 is connected with the high-energy ultraviolet xenon lamp 7, the filter disc rotating wheel driver 8 is respectively connected with the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filter rotating wheel assembly 12, the high-frequency pulse laser power supply 17 is connected with the high-frequency pulse laser 18, the time-dependent single photon counter 27 is respectively connected with the high-frequency pulse laser power supply 17 and the photomultiplier 26, the embedded industrial personal computer 28 is respectively connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter disc rotating wheel driver 8, the time-dependent single photon counter 27 and the circulating pump 5, and the on-line monitoring software 29 is located on the embedded industrial personal computer 28.
In this embodiment, the high-energy ultraviolet xenon lamp power supply 6 is connected with the high-energy ultraviolet xenon lamp 7 through a control cable, the high-frequency pulse laser power supply 17 is connected with the high-frequency pulse laser 18 through a control cable, and the filter wheel driver 8 is respectively connected with the 15-bit narrow-band filter wheel assembly 9 and the 15-bit chopping filter wheel assembly 12 through a control cable. The time-dependent single photon counter 27 is connected to the high frequency pulsed laser power supply 17 and the photomultiplier 26, respectively, by control cables. The embedded industrial personal computer 28 is respectively connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter disc rotating wheel driver 8, the time-dependent single photon counter 27 and the circulating pump 5 through control cables. The on-line monitoring software 29 is installed on the embedded industrial personal computer 28. The filter disc rotating wheel driver 8 is respectively connected with the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 through control cables, so that the rotation of each rotating wheel assembly can be independently controlled, and meanwhile, the synchronous rotation of the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 can be accurately controlled, and the accuracy of excitation-fluorescence spectrum signals obtained by the multispectral detection system is greatly ensured. The time-dependent single photon counter 27 is connected with the high-frequency pulse laser power supply 17 and the photomultiplier 26 through control cables respectively, so that the timing precision is greatly ensured, and the accuracy of fluorescence lifetime detection is improved. The on-line monitoring software 29 is installed on the embedded industrial personal computer 28, and is connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter disc rotating wheel driver 8, the time-related single photon counter 27 and the circulating pump 5 through control cables of the embedded industrial personal computer 28, so that the working states of all instruments are obtained in real time, the information of all instruments is mastered in time, and the guarantee is provided for the normal operation of the whole instrument.
In this embodiment, the working flow of the on-line monitor for atmospheric microorganisms is specifically as follows:
after the on-line monitor of the atmospheric microorganisms is started, on-line monitoring software 29 firstly runs a gas collecting system, on-line monitoring software 29 on the embedded industrial personal computer 28 firstly controls and starts the circulating pump 5, and the atmospheric microorganisms enter the gas collecting tube 3 for sample collection through the drainage tube 2 after being filtered by the coarse screen 1 under the negative pressure effect of the circulating pump 5 through the pump suction tube 4.
After the gas collecting system operates stably, the online monitoring software 29 controls the multispectral detection system to start operating, and the specific flow is as follows: the on-line monitoring software 29 controls the filter disc rotating wheel driver 8 to obtain the position information of the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 through the embedded industrial personal computer 28, controls the filter disc rotating wheel driver 8 to respectively drive the 15-bit narrow-band filter rotating wheel assembly 9 to rotate to a 390nm narrow-band filter, and drives the 15-bit chopping filtering rotating wheel assembly 12 to rotate to a 400nm long-wave pass filter, so that the rotating wheel position initialization is completed. After the initialization of the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 is completed, the on-line monitoring software 29 controls the high-energy ultraviolet xenon lamp power supply 6 to start the high-energy ultraviolet xenon lamp 7 through the embedded industrial personal computer 28, the light beam of the high-energy ultraviolet xenon lamp 7 is filtered after passing through the 15-bit narrow-band filter rotating wheel assembly 9, only 390nm excitation light is left, the 390nm excitation light is focused and irradiated on the atmospheric microorganisms in the gas collecting tube 3, and the atmospheric microorganisms in the gas collecting tube 3 are excited to generate fluorescence spectrum signals. The fluorescence spectrum signals are received by the first condenser lens 10 on the side of the gas collecting tube 3, transmitted to the first plano-convex lens 11, converted into parallel beams by the first plano-convex lens 11, filtered by a 400nm long-wave pass filter on the 15-bit chopper filter rotating wheel assembly 12 to obtain fluorescence spectrum signals smaller than 400nm, transmitted to the second plano-convex lens 13, focused by the second plano-convex lens 13 into the multimode optical fiber 15 on the optical fiber connecting plate 14, transmitted to the multi-spectral detector 16 by the multimode optical fiber 15, subjected to signal processing, transmitted to the embedded industrial personal computer 28, further processed by the on-line monitoring software 29, displayed on a display interface of the on-line monitoring software 29, and finally initially detected by the multi-spectral detection system.
After the initial detection of the multispectral detection system is completed, the on-line monitoring software 29 controls the filter disc rotating wheel driver 8 to drive the 15-bit narrow-band filter disc rotating wheel assembly 9 to rotate clockwise to a 250nm narrow-band filter disc gear through the embedded industrial personal computer 28, and synchronously drives the 15-bit chopping filter disc rotating wheel assembly 12 to rotate clockwise to a 260nm long-wave pass filter disc gear. At this time, the high-energy ultraviolet xenon lamp 7 focuses on the atmospheric microorganisms in the gas collecting tube 3 through the 15-bit narrow-band filter runner assembly 9, excitation light mainly is 250nm excitation light, scattered fluorescence generated by exciting the atmospheric microorganisms by the 250nm excitation light is received by the first collecting lens 10 and focused and transmitted to the first plano-convex lens 11, the first plano-convex lens 11 converts parallel light and then transmits the parallel light to a 260nm long-wave pass filter of the 15-bit chopping filter runner assembly 12, the 260nm long-wave pass filter filters fluorescence smaller than 260nm, a fluorescence signal larger than 260nm is focused into the multimode optical fiber 15 connected with the optical fiber connecting plate 14 by the second plano-convex lens 13, and then the multimode optical fiber 15 transmits the fluorescence signal to the multispectral detector 16 for signal processing and is transmitted to the embedded industrial personal computer 28 for processing and display by the on-line monitoring software 29, so that the 250nm wavelength spectrum detection is completed. The detection flow of the wavelength spectrum of 250nm is circulated, and then the on-line monitoring software 29 controls the filter disc rotating wheel driver 8 to synchronously drive the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 to rotate clockwise to 260nm narrow-band filter and 270nm long-wave pass filter files through the embedded industrial personal computer 28, so that the detection of the wavelength spectrum of 260nm is completed; driving the 15-bit narrow-band filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 to rotate clockwise to a 270nm narrow-band filter and a 280nm long-wave pass filter to finish 270nm wavelength spectrum detection; … … until the 15-bit narrow-band filter runner assembly 9 and the 15-bit chopping filter runner assembly 12 are driven to rotate clockwise to 390nm narrow-band filter and 400nm long-wave pass filter, 390nm wavelength spectrum detection is completed, and thus a round of 15-path excitation-fluorescence spectrum detection from 250nm to 390nm is completed.
The data obtained by completing a round of detection of the 15-path wavelength spectrum from 250nm to 390nm are transmitted to the embedded industrial personal computer 28 and processed and displayed by the on-line monitoring software 29. Meanwhile, the on-line monitoring software 29 also automatically inverts data obtained according to a round of 15-path wavelength spectrum detection from 250nm to 390nm to generate a multi-wavelength excitation-fluorescence spectrogram (EEM) for display on the on-line monitoring software 29.
While controlling the operation of the multi-spectral detection system, the on-line monitoring software 29 also controls the synchronous operation of the fluorescence lifetime detection system. The operation flow of the fluorescence lifetime detection system is as follows: the on-line monitoring software 29 controls the high-frequency pulse laser power supply 17 to start the high-frequency pulse laser 18 through the embedded industrial personal computer 28, and laser emitted by the high-frequency pulse laser 18 passes through the high-energy optical filter 20 and then is focused on atmospheric microorganisms in the gas collecting tube 3 through the third plano-convex mirror 21 to excite the atmospheric microorganisms in the gas collecting tube 3 to generate fluorescent signals. The fluorescent signal generated in the gas collecting tube 3 is received by the second condenser 22, condensed and projected onto the fourth plano-convex lens 23, converted into parallel light by the fourth plano-convex lens 23 and transmitted to the long wave filter 24, the long wave filter 24 filters out fluorescent light lower than the center wavelength, the fluorescent light higher than the center wavelength is transmitted to the fifth plano-convex lens 25, and the fluorescent light is focused onto the sensing surface of the photomultiplier 26 by the fifth plano-convex lens 25. The photomultiplier 26 converts the obtained optical signal into an electrical signal, the electrical signal is amplified by an internal amplifier and then transmitted to a time-dependent single photon counter 27, and the time-dependent single photon counter 27 transmits the obtained data of the high-frequency pulse laser power supply 17 and the photomultiplier 26 to an embedded industrial personal computer 28 for storage, and the data is further processed by an on-line monitoring software 29 and then displayed. The on-line monitoring software 29 can also control the synchronous operation of the multi-channel fluorescence lifetime detection system simultaneously, and draw a multi-wavelength excitation-fluorescence lifetime graph.
The on-line monitoring software 29 can store, display and further invert the data transmitted by the multispectral detection system and the fluorescence lifetime detection system through the embedded industrial personal computer 28, and can also monitor the working states of the circulating pump 5, the high-energy ultraviolet xenon lamp power supply 6, the multispectral detector 16, the high-frequency pulse laser power supply 17, the filter disc rotating wheel driver 8 and the time-related single photon counter 27 in real time.
The on-line monitor for atmospheric microorganisms in this embodiment is characterized by mainly comprising: the system comprises a gas collecting system, a multispectral detection system, a fluorescence service life detection system and a high-speed data acquisition system. The gas collecting system provides possibility for online monitoring, ensures that the instrument can collect atmospheric microorganisms online in real time, and can be further expanded into real-time online collection of atmospheric pollution, haze, environmental pollution, water pollution and the like. The excitation wavelength of the multispectral detection system almost covers the whole ultraviolet band (from 250nm to 390 nm), the receiving wavelength almost completely covers the fluorescence spectrum band (from 260nm to 900 nm), and the generated multi-wavelength excitation-fluorescence spectrum chart (EEM) provides possibility for identifying more kinds of bacteria, viruses and other microorganisms. The fluorescence life detection system is used as an auxiliary detection system of the multispectral detection system, so that the accumulation of environmental data of microorganisms is ensured, powerful guarantee is provided for timely grasping the characteristic analysis of the survival environment of the microorganisms, and powerful guidance is provided for the disinfection of harmful microorganisms; the fluorescence lifetime detection system also provides a multi-channel expansion module, which is convenient to expand and adjust according to different application scenes. The high-speed data acquisition system realizes real-time analysis inversion of data obtained by the multispectral detection system and the fluorescence lifetime detection system, generates a timely data report, and is convenient to check; the high-speed data acquisition system can also timely early warn detected abnormal microorganisms and environmental changes, so that invasion of harmful microorganisms can be timely killed and effective measures can be timely taken for protection. Meanwhile, the multispectral detection system and the fluorescence lifetime detection system are installed on two sides of the gas collecting system in a layered mode, and the space utilization rate of the instrument and the utilization efficiency of the gas collecting system are greatly improved.
The foregoing is merely a preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions easily conceivable by those skilled in the art within the technical scope of the present application should be covered in the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (6)
1. The atmospheric microorganism on-line monitor is characterized by comprising a gas collecting system, a multispectral detection system, a fluorescence service life detection system and a high-speed data acquisition system;
the gas collecting system is used for collecting atmospheric microorganisms in real time;
the gas collecting system comprises: the device comprises a coarse filter screen (1), a drainage tube (2), a gas collecting tube (3), a pump suction tube (4) and a circulating pump (5);
the rough filter screen (1) is connected with an air inlet of the gas collecting tube (3) through the drainage tube (2), and an air outlet of the gas collecting tube (3) is connected with the circulating pump (5) through the pump suction tube (4);
the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals;
the multispectral detection system includes: the high-energy ultraviolet xenon lamp (7), a 15-bit narrow-band optical filter rotating wheel assembly (9), a first collecting lens (10), a first plano-convex lens (11), a 15-bit chopping optical filter rotating wheel assembly (12), a second plano-convex lens (13), an optical fiber connecting plate (14), multimode optical fibers (15) and a multispectral detector (16);
the high-energy ultraviolet xenon lamp (7) and the 15-bit narrow-band filter rotating wheel assembly (9) are positioned on one side of the gas collecting tube (3), and the 15-bit narrow-band filter rotating wheel assembly (9) is positioned on the optical axis of the high-energy ultraviolet xenon lamp (7);
the first collecting lens (10), the first plano-convex lens (11), the 15-bit chopping filtering rotating wheel assembly (12), the second plano-convex lens (13) and the optical fiber connecting plate (14) are coaxially positioned on the other side of the gas collecting tube (3) in sequence and positioned in the non-coaxial direction of the high-energy ultraviolet xenon lamp (7);
the optical fiber connecting plate (14) is connected with the multispectral detector (16) through the multimode optical fiber (15);
the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals;
the fluorescence lifetime detection system comprises: the high-frequency pulse laser (18), a high-energy optical filter (20), a third plano-convex mirror (21), a second condenser (22), a fourth plano-convex mirror (23), a long-wave transmitting optical filter (24), a fifth plano-convex mirror (25) and a photomultiplier (26);
the high-frequency pulse laser (18), the high-energy optical filter (20) and the third plano-convex mirror (21) are positioned on one side of the gas collecting tube (3);
the high-energy optical filter (20) and the third plano-convex mirror (21) are positioned in the emission direction of the emission optical axis of the high-frequency pulse laser (18), and the third plano-convex mirror (21) is focused at the center line position of the gas collecting tube (3);
the second condenser (22), the fourth plano-convex lens (23), the long-wave-transmitting optical filter (24), the fifth plano-convex lens (25) and the photomultiplier (26) are coaxially positioned on the other side of the gas collecting tube (3) in sequence and positioned in a non-coaxial direction of the emission optical axis of the high-frequency pulse laser (18);
the axis of the second condenser (22) is in the same plane with the axis of the high-frequency pulse laser (18) and perpendicularly intersects with the central line of the gas collecting tube (3);
the high-speed data acquisition system is used for carrying out analysis inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram, and generating a data report.
2. The online monitor for atmospheric microorganisms according to claim 1, wherein the optical axes of the first condenser (10) and the first plano-convex lens (11) are in the same plane with the optical axis of the high-energy ultraviolet xenon lamp (7) and are perpendicular to the central line of the gas collecting tube (3).
3. The online monitor for atmospheric microorganisms according to claim 1, wherein the high-frequency pulse laser (18), the high-energy optical filter (20) and the third plano-convex mirror (21) are positioned on the same side of the gas collecting tube (3) as the high-energy ultraviolet xenon lamp (7) and the 15-bit narrow-band optical filter runner assembly (9) and are arranged in a layered manner.
4. The atmospheric microorganisms online monitor of claim 1, wherein the high-speed data acquisition system comprises: the device comprises a high-energy ultraviolet xenon lamp power supply (6), a high-frequency pulse laser power supply (17), a filter disc rotating wheel driver (8), a time-related single photon counter (27), an embedded industrial personal computer (28) and on-line monitoring software (29);
the high-energy ultraviolet xenon lamp power supply (6) is connected with the high-energy ultraviolet xenon lamp (7), the filter disc rotating wheel driver (8) is respectively connected with the 15-bit narrow-band filter rotating wheel assembly (9) and the 15-bit chopping filter rotating wheel assembly (12), the high-frequency pulse laser power supply (17) is connected with the high-frequency pulse laser (18), the time-dependent single photon counter (27) is respectively connected with the high-frequency pulse laser power supply (17) and the photomultiplier (26), the embedded industrial personal computer (28) is respectively connected with the high-energy ultraviolet xenon lamp power supply (6), the high-frequency pulse laser power supply (17), the filter disc rotating wheel driver (8), the time-dependent single photon counter (27) and the circulating pump (5), and the on-line monitoring software (29) is located on the embedded industrial personal computer (28).
5. The online monitor for atmospheric microorganisms according to claim 4, wherein the 15-bit narrow-band filter wheel assembly (9) is provided with a total of 15 narrow-band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm in turn anticlockwise;
a total of 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering rotating wheel assembly (12) in a anticlockwise mode.
6. The on-line monitor for atmospheric microorganisms according to claim 5, wherein,
the narrow-band filter on the 15-bit narrow-band filter rotating wheel assembly (9) and the long-wave pass filter on the 15-bit chopping filter rotating wheel assembly (12) are sequentially corresponding to each other according to a counterclockwise installation sequence;
the on-line monitoring software (29) controls the filter disc rotating wheel driver (8) to drive the 15-bit narrow-band filter rotating wheel assembly (9) to rotate clockwise through the embedded industrial personal computer (28), and synchronously drives the 15-bit chopping filtering rotating wheel assembly (12) to rotate clockwise.
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