CN114965400A - Atmospheric microorganism on-line monitor - Google Patents
Atmospheric microorganism on-line monitor Download PDFInfo
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Abstract
The invention provides an atmospheric microorganism on-line monitor, which comprises a gas collection system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system, wherein the multispectral detection system is connected with the gas collection system; the gas collection system is used for collecting atmospheric microorganisms in real time; the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals; the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals; the high-speed data acquisition system is used for carrying out analysis and inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram and generate a data report. The invention realizes real-time monitoring of the variety and concentration change of the atmospheric microorganisms, inversion and result presentation in time, and early warning analysis in time for the abnormal atmospheric microorganisms.
Description
Technical Field
The invention belongs to the technical field of atmospheric environment monitoring, and particularly relates to an atmospheric microorganism online monitor.
Background
The atmosphere is an important environment for human life and is closely related to human production and life. The control of atmospheric composition and concentration change is especially necessary for guidance and daily life of people. And atmospheric microorganisms change at the moment along with the change of atmosphere. The mastering of the variety, content and change rule of microorganisms in the atmosphere is one of the important problems in the world today. Especially, in epidemic situation, real-time online monitoring of harmful bacteria, viruses and other microorganisms in the atmosphere is very important and necessary.
At present, the instruments for monitoring cloud, dust, haze, water vapor, nitrogen, ozone and the like in the atmosphere are more and mature, and the real-time online monitoring is basically realized. For monitoring of atmospheric microorganisms, detection is basically performed in an off-line manner, i.e., an air sample is first collected by using a liquid or solid sampler, and then identified, identified or detected by various means in a laboratory according to needs or purposes. The method has the problems of low timeliness, high cost, complex operation and the like, and a large amount of manpower, material resources and financial resources are required to be input. In recent years, a fluorescence laser radar is widely used as an atmospheric microorganism on-line monitoring instrument, but the fluorescence laser radar is influenced by a detection blind area and cannot detect the surface atmosphere close to the work and life of people or the indoor environment just in the blind area range, so that the monitored data cannot reliably reflect the change of atmospheric microorganisms living by people, and the fluorescence laser radar needs a high-energy laser as a light source, so that the instrument needs a relatively harsh environment to observe. In particular, fluorescence lidar often emits only 1-2 excitation uv lasers or detects only a certain band or range of continuous fluorescence spectra, and does not have a full excitation/emission fluorescence spectrum (EEM), resulting in interference or influence from other substance fluorescence signals when identifying/identifying atmospheric microorganisms.
Disclosure of Invention
In order to solve the technical problems, the invention provides an online atmospheric microorganism monitor, which can monitor the variety and concentration change of atmospheric microorganisms in real time, carry out inversion and result presentation in time and carry out early warning analysis on abnormal atmospheric microorganisms in time.
In order to realize the aim, the invention provides an atmospheric microorganism on-line monitor, which comprises a gas collection system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system;
the gas collection system is used for collecting atmospheric microorganisms in real time;
the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals;
the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals;
and the high-speed data acquisition system is used for carrying out analysis and inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum graph and a multi-wavelength excitation-fluorescence lifetime graph and generate a data report.
Optionally, the gas collection system comprises: a coarse filter screen, a drainage tube, a gas collecting tube, a pump suction tube and a circulating pump;
the coarse strainer is connected with the air inlet of the air collecting pipe through the drainage pipe, and the air outlet of the air collecting pipe is connected with the circulating pump through the pump suction pipe.
Optionally, the multispectral detection system comprises: the system comprises a high-energy ultraviolet xenon lamp, a 15-bit narrowband filter rotating wheel component, a first condenser lens, a first plano-convex lens, a 15-bit chopping filtering rotating wheel component, a second plano-convex lens, an optical fiber connecting plate, a multimode optical fiber and a multispectral detector;
the high-energy ultraviolet xenon lamp and the 15-bit narrow-band optical filter rotating wheel component are positioned on one side of the gas collecting tube, and the 15-bit narrow-band optical filter rotating wheel component is positioned on an optical axis of the high-energy ultraviolet xenon lamp;
the first condenser lens, the first plano-convex lens, the 15-bit chopping filtering rotating wheel assembly, the second plano-convex lens and the optical fiber connecting plate are coaxially arranged on the other side of the gas collecting tube in sequence and are arranged in the non-coaxial direction of the high-energy ultraviolet xenon lamp;
the optical fiber connecting plate is connected with the multispectral detector through the multimode optical fiber.
Optionally, the optical axes of the first condenser and the first plano-convex mirror and the optical axis of the high-energy ultraviolet xenon lamp are on the same plane and perpendicular to the central line of the gas collecting tube.
Optionally, the fluorescence lifetime detection system comprises: the high-frequency pulse laser, the high-energy optical filter, the third plano-convex lens, the second condenser, the fourth plano-convex lens, the long-wave transmission optical filter, the fifth plano-convex lens and the photomultiplier;
the high-frequency pulse laser, the high-energy optical filter and the third plano-convex lens are positioned on one side of the gas collecting pipe;
the high-energy optical filter and the third plano-convex mirror are positioned in the emission direction of the emission optical axis of the high-frequency pulse laser, and the third plano-convex mirror is focused on the middle line position of the gas collecting pipe.
The second condenser, the fourth plano-convex lens, the long-wave transmission optical filter, the fifth plano-convex lens and the photomultiplier are coaxially arranged on the other side of the gas collecting tube in sequence and are positioned in the non-coaxial direction of the emission optical axis of the high-frequency pulse laser;
the axis of the second condenser is in the same plane with the axis of the high-frequency pulse laser and is vertically intersected with the central line of the gas collecting pipe.
Optionally, the high-frequency pulse laser, the high-energy optical filter, the third plano-convex mirror, the high-energy ultraviolet xenon lamp and the 15-bit narrow-band optical filter rotating wheel assembly are located on the same side of the gas collecting tube and are arranged in layers.
Optionally, the high speed data acquisition system comprises: the system comprises a high-energy ultraviolet xenon lamp power supply, a high-frequency pulse laser power supply, a filter disc runner driver, a time-dependent single photon counter, an embedded industrial personal computer and online monitoring software;
the high-energy ultraviolet xenon lamp power supply is connected with the high-energy ultraviolet xenon lamp, the filter rotating wheel driver is respectively connected with the 15-bit narrow-band filter rotating wheel component and the 15-bit chopping filtering rotating wheel component, the high-frequency pulse laser power supply is connected with the high-frequency pulse laser, the time-dependent single photon counter is respectively connected with the high-frequency pulse laser power supply and the photomultiplier, the embedded industrial personal computer is respectively connected with the high-energy ultraviolet xenon lamp power supply, the high-frequency pulse laser power supply, the filter rotating wheel driver, the time-dependent single photon counter and the circulating pump, and the online monitoring software is located on the embedded industrial personal computer.
Optionally, 15 narrow band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially mounted on the 15-bit narrow band filter rotating wheel assembly anticlockwise;
15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering rotating wheel component in a counterclockwise manner.
Optionally, the narrow band filters on the 15-bit narrow band filter rotating wheel assembly and the long-wave pass filters on the 15-bit chopping filtering rotating wheel assembly correspond to each other in sequence according to a counterclockwise installation sequence;
the online monitoring software controls the filter rotating wheel driver to drive the 15-bit narrow-band filter rotating wheel component to rotate clockwise through the embedded industrial personal computer, and synchronously drives the 15-bit chopping filter rotating wheel component to rotate clockwise.
Compared with the prior art, the invention has the following advantages and technical effects:
the invention provides an on-line monitor specially for atmospheric microorganisms, which is characterized in that the atmospheric microorganisms are input into an instrument in real time through an input type detection method, the input atmospheric microorganisms are respectively excited by ultraviolet light of a plurality of wave bands to obtain fluorescence spectrum signals, and the collected fluorescence spectrum signals are further analyzed and processed to form a multi-wavelength excitation-fluorescence spectrum (EEM) and fluorescence life, so that the identification and concentration measurement of microorganisms such as bacteria, viruses and the like in the atmosphere are achieved, a data report is generated, and the timely early warning is carried out according to the types and concentration changes of the atmospheric microorganisms in the data report, thereby facilitating the taking of measures for timely killing harmful microorganisms and protecting the harmful microorganisms. Meanwhile, the harm of harmful microorganisms to sampling personnel is also avoided by real-time online sampling.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application. In the drawings:
FIG. 1 is a schematic view of an atmospheric microorganism on-line monitor according to an embodiment of the present invention;
wherein: 1. coarse filtration; 2. a drainage tube; 3. a gas collecting pipe; 4. a pump suction pipe; 5. a circulation pump; 6. a high-energy ultraviolet xenon lamp power supply; 7. a high energy uv xenon lamp; 8. a filter disc rotary wheel driver; 9. 15 bit narrow band filter rotating wheel components; 10. a first condenser lens; 11. a first plano-convex mirror; 12. 15 bit chopping filtering rotating wheel components; 13. a second plano-convex mirror; 14. an optical fiber connection plate; 15. a multimode optical fiber; 16. a multispectral detector; 17. a high frequency pulsed laser power supply; 18. A high-frequency pulse laser; 20. a high-energy optical filter; 21. a third plano-convex mirror; 22. a second condenser lens; 23. a fourth plano-convex mirror; 24. a long-wave transmission filter; 25. a fifth plano-convex mirror; 26. a photomultiplier tube; 27. a time-dependent single photon counter; 28. an embedded industrial personal computer; 29. and (4) online monitoring software.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.
It should be noted that the steps illustrated in the flowcharts of the figures may be performed in a computer system such as a set of computer-executable instructions and that, although a logical order is illustrated in the flowcharts, in some cases, the steps illustrated or described may be performed in an order different than presented herein.
Examples
As shown in FIG. 1, the embodiment provides an atmospheric microorganism on-line monitor, which comprises a gas collection system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system; the gas collection system is used for collecting atmospheric microorganisms in real time; the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals; the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals; the high-speed data acquisition system is used for carrying out analysis and inversion according to the fluorescence spectrum signal data and the fluorescence lifetime signal data to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram and generate a data report.
Wherein, gas collection system includes: a coarse strainer 1, a drainage tube 2, a gas collecting tube 3, a pump suction tube 4 and a circulating pump 5;
in this embodiment, the coarse filter screen 1 is connected to the air inlet of the air collecting pipe 3 through the drainage pipe 2, and the air outlet of the air collecting pipe 3 is connected to the circulating pump 5 through the pumping pipe 4.
The coarse filter 1 blocks large particle impurities, flies, insects and the like from entering the system, so as to prevent the blockage of the gas collecting system, and the drainage tube 2 introduces external atmosphere into the gas collecting tube 3; the gas collecting pipe 3 is an important online structure, and a device for preventing microorganisms from flowing through rapidly is arranged in the gas collecting pipe 3, so that the microorganisms with enough concentration are always gathered in the gas collecting pipe 3 for monitoring. The circulating pump 5 provides transmission power for the whole gas collection system through the pump suction pipe 4.
The multispectral detection system comprises: the system comprises a high-energy ultraviolet xenon lamp 7, a 15-bit narrowband filter rotating wheel component 9, a first condenser 10, a first plano-convex mirror 11, a 15-bit chopping filtering rotating wheel component 12, a second plano-convex mirror 13, an optical fiber connecting plate 14, a multimode optical fiber 15 and a multispectral detector 16;
the high-energy ultraviolet xenon lamp 7 and the 15-bit narrow-band filter rotating wheel assembly 9 are positioned on one side of the gas collecting tube 3, and the 15-bit narrow-band filter rotating wheel assembly 9 is positioned on the optical axis of the high-energy ultraviolet xenon lamp 7, is positioned on the same plane with the optical axis of the high-energy ultraviolet xenon lamp 7 and is vertical to the central line of the gas collecting tube 3; the first condenser 10, the first plano-convex mirror 11, the 15-bit chopping filtering rotating wheel assembly 12, the second plano-convex mirror 13 and the optical fiber connecting plate 14 are coaxially arranged on the other side of the gas collecting pipe 3 in sequence and are arranged in the non-coaxial direction of the high-energy ultraviolet xenon lamp 7; the optical fiber connection board 14 is connected with the multispectral detector 16 through the multimode optical fiber 15.
In the embodiment, the high-energy ultraviolet xenon lamp 7 is condensed on the middle line of the gas collecting tube 3, and the 15-bit narrow-band filter rotating wheel assembly 9 is arranged on the optical axis of the high-energy ultraviolet xenon lamp 7 between the high-energy ultraviolet xenon lamp 7 and the gas collecting tube 3. The multi-spectral detector is characterized in that the multi-spectral detector is perpendicular to the center line of the gas collecting tube 3, is positioned on the same plane with the optical axis of the high-energy ultraviolet xenon lamp 7, is sequentially coaxially provided with a first collecting lens 10, a first plano-convex lens 11, a 15-bit chopping and filtering rotating wheel assembly 12, a second plano-convex lens 13 and an optical fiber connecting plate 14 in the non-coaxial direction of the optical axis of the high-energy ultraviolet xenon lamp 7 on the other side of the gas collecting tube 3, and the optical fiber connecting plate 14 is connected with a multi-spectral detector 16 through a multi-mode optical fiber 15. Wherein 15 narrow-band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially arranged on the 15-bit narrow-band filter rotating wheel component 9 anticlockwise; 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering rotating wheel component 12 in a counterclockwise manner.
The high-energy ultraviolet xenon lamp 7 is used as an important excitation light source of a multispectral detection system, so that the output light beam of the high-energy ultraviolet xenon lamp has high energy distribution in an ultraviolet band, the xenon lamp with internal arc reflection is adopted, the utilization efficiency of the xenon lamp is greatly improved, the light beam output by the high-energy ultraviolet xenon lamp 7 is directly focused to one point, and the optical filter is arranged in front of the focus of the light beam, so that the volume of an instrument is greatly reduced, and the space utilization rate is improved. 15 narrow-band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are sequentially arranged on a 15-bit narrow-band filter rotating wheel component 9 anticlockwise, and 15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on a 15-bit chopping wave filter rotating wheel component 12 anticlockwise to synchronously act, so that the one-to-one correspondence of exciting light and fluorescence spectrum signals obtained by excitation is ensured. The use of the first condenser lens 10 ensures that a sufficient amount of the fluorescent signal is received, and the use of the second plano-convex lens 13, the optical fiber patch 14 and the multimode optical fiber 15 together allows the system to be more compact. The multispectral detector 16 is an important collection system of the multispectral detection system, and a multi-wavelength fluorescence spectrum collector such as a P32 multispectral laser radar detector of the german licel company is adopted, so that detection of a wider fluorescence spectrum signal is better realized. Meanwhile, the optical axes of the first condenser lens 10 and the high-energy ultraviolet xenon lamp 7 are arranged on the same plane, so that the first condenser lens 10 can obtain more strong fluorescence spectrum signals, and the signal quality is greatly improved; the first condenser 10 and the high-energy ultraviolet xenon lamp 7 are arranged on the side surface of the gas collecting tube 3 in a non-coaxial mode, damage to the multispectral detector 16 and the like caused by strong light irradiation in the coaxial installation is avoided, and the service life of the instrument is greatly prolonged.
The fluorescence lifetime detection system includes: a high-frequency pulse laser 18, a high-energy optical filter 20, a third plano-convex mirror 21, a second condenser 22, a fourth plano-convex mirror 23, a long-wave transmission optical filter 24, a fifth plano-convex mirror 25 and a photomultiplier 26;
the high-frequency pulse laser 18, the high-energy optical filter 20 and the third plano-convex mirror 21 are positioned at one side of the gas collecting pipe 3; the high-energy optical filter 20 and the third plano-convex mirror 21 are located in the emission direction of the emission optical axis of the high-frequency pulse laser 18, and the third plano-convex mirror 21 is focused on the central line position of the gas collecting tube 3 and is located on the same plane as the emission optical axis of the high-frequency pulse laser 18; the second condenser 22, the fourth plano-convex lens 23, the long-wave transmission filter 24, the fifth plano-convex lens 25 and the photomultiplier 26 are coaxially positioned on the other side of the gas collecting tube 3 in sequence and are positioned in the non-coaxial direction of the emission optical axis of the high-frequency pulse laser 18; the axis of the second condenser 22 perpendicularly intersects the center line of the gas collecting pipe 3.
The high-frequency pulse laser 18, the high-energy optical filter 20, the third plano-convex mirror 21 and the high-energy ultraviolet xenon lamp 7 and 15-bit narrow-band optical filter rotating wheel assembly 9 are positioned on the same side of the gas collecting tube 3 and are arranged in a layered mode.
In the present embodiment, a high-energy optical filter 20 and a third plano-convex mirror 21 are sequentially arranged in the emission direction of the emission optical axis of the high-frequency pulse laser 18, and the third plano-convex mirror 21 is focused at the central line position of the gas collecting tube 3; the second condenser 22, the fourth plano-convex mirror 23, the long-wave transmission filter 24, the fifth plano-convex mirror 25 and the photomultiplier 26 are coaxially arranged in sequence on the same plane perpendicular to the center line of the gas collecting tube 3 and on the same plane with the emission optical axis of the high-frequency pulse laser 18 on the other side of the gas collecting tube 3 and in the direction of non-coaxial with the optical axis of the high-frequency pulse laser 18, and the axis of the second condenser 22 intersects with the center line of the gas collecting tube 3. The fluorescence lifetime detection system is provided with an extension port and can be extended into simultaneous detection of multiple paths of fluorescence lifetimes as required. The high-frequency pulse laser 18 adopts a femtosecond laser or a picosecond laser with high repetition frequency, and the precision of the fluorescence life detection is greatly improved. The photomultiplier tube 26 may employ a PMT or APD with an internal amplifier and temperature control, greatly improving detection accuracy and sensitivity. Meanwhile, the second condenser 22 and the high-frequency pulse laser 18 are installed on the same plane, so that the second condenser 22 can obtain more strong fluorescence life signals, and the signal quality is greatly improved; the second condenser 22 and the high-frequency pulse laser 18 are arranged on the side of the gas collecting tube 3 in a non-coaxial mode, damage to the photomultiplier 26 and the like caused by strong light irradiation of coaxial arrangement is avoided, and the service life of the device is greatly prolonged.
The high-speed data acquisition system comprises: a high-energy ultraviolet xenon lamp power supply 6, a high-frequency pulse laser power supply 17, a filter disc rotating wheel driver 8, a time-related single photon counter 27, an embedded industrial personal computer 28 and online monitoring software 29;
the high-energy ultraviolet xenon lamp power supply 6 is connected with the high-energy ultraviolet xenon lamp 7, the filter rotating wheel driver 8 is respectively connected with the 15-bit narrow-band filter rotating wheel component 9 and the 15-bit chopping filter rotating wheel component 12, the high-frequency pulse laser power supply 17 is connected with the high-frequency pulse laser 18, the time-dependent single photon counter 27 is respectively connected with the high-frequency pulse laser power supply 17 and the photomultiplier 26, the embedded industrial personal computer 28 is respectively connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter rotating wheel driver 8, the time-dependent single photon counter 27 and the circulating pump 5, and the online monitoring software 29 is positioned on the embedded industrial personal computer 28.
In this embodiment, the high-energy ultraviolet xenon lamp power supply 6 is connected with the high-energy ultraviolet xenon lamp 7 through a control cable, the high-frequency pulse laser power supply 17 is connected with the high-frequency pulse laser 18 through a control cable, and the filter rotating wheel driver 8 is respectively connected with the 15-bit narrowband filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 through control cables. The time-correlated single photon counter 27 is connected with the high-frequency pulse laser power supply 17 and the photomultiplier 26 through control cables, respectively. The embedded industrial personal computer 28 is respectively connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter rotating wheel driver 8, the time-related single photon counter 27 and the circulating pump 5 through control cables. The online monitoring software 29 is installed on the embedded industrial personal computer 28. The filter rotating wheel driver 8 is respectively connected with the 15-bit narrowband filter rotating wheel component 9 and the 15-bit chopping filtering rotating wheel component 12 through control cables, so that the rotation of each rotating wheel component can be independently controlled, the synchronous rotation of the 15-bit narrowband filter rotating wheel component 9 and the 15-bit chopping filtering rotating wheel component 12 can be accurately controlled, and the accuracy of the multispectral detection system for obtaining excitation-fluorescence spectrum signals is greatly guaranteed. The time-related single photon counter 27 is respectively connected with the high-frequency pulse laser power supply 17 and the photomultiplier 26 through control cables, so that the timing precision is greatly ensured, and the accuracy of fluorescence life detection is improved. The online monitoring software 29 is installed on the embedded industrial personal computer 28, and is respectively connected with the high-energy ultraviolet xenon lamp power supply 6, the high-frequency pulse laser power supply 17, the filter rotating wheel driver 8, the time-dependent single photon counter 27 and the circulating pump 5 through control cables of the embedded industrial personal computer 28, so that the working state of each instrument can be obtained in real time, the information of each instrument can be timely mastered, and the normal operation of the whole instrument can be guaranteed.
In this embodiment, the working process of the online atmospheric microorganism monitor is as follows:
after the on-line air microorganism monitor is started, the on-line monitoring software 29 firstly operates the gas collecting system, the on-line monitoring software 29 on the embedded industrial personal computer 28 firstly controls the circulating pump 5 to be started, and the air microorganisms are filtered and decontaminated by the coarse filter screen 1 under the negative pressure action of the circulating pump 5 through the pump suction pipe 4 and then enter the gas collecting pipe 3 through the drainage pipe 2 for sample collection.
After the gas collection system operates stably, the online monitoring software 29 controls the multispectral detection system to start to operate, and the specific flow is as follows: the online monitoring software 29 controls the filter rotating wheel driver 8 to obtain the position information of the 15-bit narrowband filter rotating wheel component 9 and the 15-bit chopping filtering rotating wheel component 12 through the embedded industrial personal computer 28, controls the filter rotating wheel driver 8 to respectively drive the 15-bit narrowband filter rotating wheel component 9 to rotate to the 390nm narrowband filter, and drives the 15-bit chopping filtering rotating wheel component 12 to rotate to the 400nm long-wave pass filter, and completes the initialization of the rotating wheel position. After the 15-bit narrowband filter rotating wheel assembly 9 and the 15-bit chopping filtering rotating wheel assembly 12 are initialized, the online monitoring software 29 controls the high-energy ultraviolet xenon lamp power supply 6 to start the high-energy ultraviolet xenon lamp 7 through the embedded industrial personal computer 28, light beams of the high-energy ultraviolet xenon lamp 7 are filtered after passing through the 15-bit narrowband filter rotating wheel assembly 9, only 390nm exciting light is left, the 390nm exciting light is focused and irradiated on atmospheric microorganisms in the gas collecting tube 3, and the atmospheric microorganisms in the gas collecting tube 3 are excited to generate fluorescence spectrum signals. The fluorescence spectrum signals are received by a first condenser lens 10 at the side of the gas collecting pipe 3, transmitted to a first plano-convex lens 11 and converted into parallel light beams by the first plano-convex lens 11, the parallel light beams behind the first plano-convex lens 11 pass through a 400nm long-wave pass filter on a 15-bit chopping filtering and rotating wheel assembly 12 to filter fluorescence spectrum signals smaller than 400nm, the fluorescence spectrum signals larger than 400nm are transmitted to a second plano-convex lens 13, the second plano-convex lens 13 is focused into a multimode optical fiber 15 on an optical fiber connecting plate 14, the multimode optical fiber 15 transmits the fluorescence signals to a multispectral detector 16 for signal processing, then the data are transmitted to an embedded industrial personal computer 28, the data are further processed by an online monitoring software 29 and then displayed on a display interface of the online monitoring software 29, and initial detection of the multispectral detection system is completed.
After the initial detection of the multispectral detection system is completed, the online monitoring software 29 controls the filter rotor driver 8 to drive the 15-bit narrowband filter rotor assembly 9 to rotate clockwise to the 250nm narrowband filter gear thereof through the embedded industrial personal computer 28, and synchronously drives the 15-bit chopping filter rotor assembly 12 to rotate clockwise to the 260nm long-wavelength bandpass filter gear thereof. At the moment, the high-energy ultraviolet xenon lamp 7 is focused on atmospheric microorganisms in the gas collecting tube 3 through the 15-bit narrow-band filter rotating wheel assembly 9, the exciting light is mainly 250nm exciting light, the 250nm exciting light excites the scattered fluorescence generated by the atmospheric microorganisms, the scattered fluorescence is received, focused and transmitted to the first plano-convex mirror 11 through the first condenser lens 10, is converted into parallel light through the first plano-convex mirror 11 and transmitted to the 260nm long-wave pass filter of the 15-bit chopping filtering rotating wheel assembly 12, the fluorescence smaller than 260nm is filtered through the 260nm long-wave pass filter, a fluorescence signal larger than 260nm is focused into the multimode optical fiber 15 connected with the optical fiber connecting plate 14 through the second plano-convex mirror 13, and then is transmitted to the multi-spectrum detector 16 through the multimode optical fiber 15 for signal processing and is transmitted to the embedded industrial personal computer 28 for processing and displaying through the online monitoring software 29, and the spectrum detection with the wavelength of 250nm is completed. The detection process of the spectrum with the wavelength of 250nm is circulated, and then the online monitoring software 29 controls the filter rotating wheel driver 8 through the embedded industrial personal computer 28 to synchronously drive the 15-bit narrow-band filter rotating wheel component 9 and the 15-bit chopping filtering rotating wheel component 12 to clockwise rotate to the gear of the 260nm narrow-band filter and the 270nm long-wave pass filter, so that the spectrum detection with the wavelength of 260nm is completed; driving a 15-bit narrowband filter rotating wheel assembly 9 and a 15-bit chopping filtering rotating wheel assembly 12 to rotate clockwise to a 270nm narrowband filter and a 280nm long-wave pass filter gear to finish 270nm wavelength spectrum detection; … … until the 15-bit narrow band filter rotating wheel assembly 9 and the 15-bit chopping filter rotating wheel assembly 12 are driven to rotate clockwise to 390nm narrow band filter and 400nm long-wave pass filter gear, 390nm wavelength spectrum detection is completed, and thus a round of 250nm to 390nm 15-path excitation-fluorescence spectrum detection is completed.
Data obtained by completing a round of detection of 15 paths of wavelength spectrums with the wavelengths of 250nm to 390nm are transmitted to the embedded industrial personal computer 28 and processed and displayed by the online monitoring software 29. Meanwhile, the online monitoring software 29 also automatically inverts data obtained according to a round of detection of 15 wavelength spectrums from 250nm to 390nm to generate a multi-wavelength excitation-fluorescence spectrum (EEM) and displays the EEM on the online monitoring software 29.
While controlling the operation of the multi-spectral detection system, the on-line monitoring software 29 also controls the synchronous operation of the fluorescence lifetime detection system. The operation flow of the fluorescence lifetime detection system is as follows: the online monitoring software 29 controls the high-frequency pulse laser power supply 17 to turn on the high-frequency pulse laser 18 through the embedded industrial personal computer 28, laser emitted by the high-frequency pulse laser 18 penetrates through the high-energy optical filter 20 and is focused on atmospheric microorganisms in the gas collecting pipe 3 through the third convex lens 21, and the atmospheric microorganisms in the gas collecting pipe 3 are excited to generate fluorescent signals. The fluorescent signal generated in the gas collecting tube 3 is received by the second condenser 22, condensed and projected onto the fourth plano-convex mirror 23, converted into parallel light by the fourth plano-convex mirror 23 and transmitted to the long-wave filter 24, the long-wave filter 24 filters the fluorescent light lower than the central wavelength, the fluorescent light higher than the central wavelength is transmitted to the fifth plano-convex mirror 25, and the fluorescent light is focused on the sensing surface of the photomultiplier 26 by the fifth plano-convex mirror 25. The photomultiplier 26 converts the obtained optical signal into an electrical signal, the electrical signal is amplified by an internal amplifier and then transmitted to a time-dependent single photon counter 27, the time-dependent single photon counter 27 transmits the obtained data of the high-frequency pulse laser power supply 17 and the photomultiplier 26 to an embedded industrial personal computer 28 for storage, and the data is further processed by online monitoring software 29 and then displayed. The on-line monitoring software 29 can also control the synchronous operation of the multi-channel fluorescence lifetime detection system and draw a multi-wavelength excitation-fluorescence lifetime map.
The online monitoring software 29 can store, display and further invert the data transmitted by the multispectral detection system and the fluorescence lifetime detection system through the embedded industrial personal computer 28, and can also monitor the working states of the circulating pump 5, the high-energy ultraviolet xenon lamp power supply 6, the multispectral detector 16, the high-frequency pulse laser power supply 17, the filter rotating wheel driver 8 and the time-dependent single photon counter 27 in real time.
The atmospheric microorganism on-line monitoring instrument in the embodiment is characterized by mainly comprising: the system comprises a gas collection system, a multispectral detection system, a fluorescence life detection system and a high-speed data acquisition system. The gas collection system provides possibility for online monitoring, ensures that the instrument can collect atmospheric microorganisms on line in real time, and can be expanded to collect atmospheric pollution, haze, environmental pollution and water pollution on line in real time. The excitation wavelength of the multi-spectrum detection system almost covers the whole ultraviolet band (from 250nm to 390nm), the receiving wavelength more completely covers the fluorescence spectrum band (from 260nm to 900nm), and the generated multi-wavelength excitation-fluorescence spectrogram (EEM) provides possibility for identifying more kinds of microorganisms such as bacteria, viruses and the like. The fluorescence lifetime detection system is used as an auxiliary detection system of the multispectral detection system, so that the accumulation of environmental data of microorganisms is guaranteed, powerful guarantee is provided for timely mastering the characteristic analysis of the survival environment of the microorganisms, and powerful guidance is provided for killing harmful microorganisms; the fluorescence lifetime detection system also provides a multi-channel expansion module, which is convenient for expansion and adjustment according to different application scenes. The high-speed data acquisition system realizes real-time analysis and inversion of data obtained by the multispectral detection system and the fluorescence life detection system, and generates a timely data report which is convenient to check; the high-speed data acquisition system can also carry out timely early warning on detected abnormal microorganisms and environmental changes, and is convenient for timely killing the invasion of harmful microorganisms and timely taking effective measures to protect. Meanwhile, the multispectral detection system and the fluorescence life detection system are installed on two sides of the gas collection system in a layered mode, and therefore the space utilization rate of the instrument and the utilization efficiency of the gas collection system are greatly improved.
The above description is only for the preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present application should be covered within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (9)
1. The atmospheric microorganism on-line monitor is characterized by comprising a gas collection system, a multispectral detection system, a fluorescence lifetime detection system and a high-speed data acquisition system;
the gas collection system is used for collecting atmospheric microorganisms in real time;
the multispectral detection system is used for exciting the atmospheric microorganisms to generate fluorescence spectrum signals;
the fluorescence lifetime detection system is used for exciting the atmospheric microorganisms to generate fluorescence lifetime signals;
the high-speed data acquisition system is used for carrying out analysis and inversion according to the fluorescence spectrum signal and the fluorescence lifetime signal to generate a multi-wavelength excitation-fluorescence spectrum diagram and a multi-wavelength excitation-fluorescence lifetime diagram and generate a data report.
2. The atmospheric microbe on-line monitor of claim 1, wherein the gas collection system comprises: a coarse filter screen (1), a drainage tube (2), a gas collecting tube (3), a pump suction tube (4) and a circulating pump (5);
the coarse filter screen (1) is connected with the air inlet of the air collecting pipe (3) through the drainage pipe (2), and the air outlet of the air collecting pipe (3) is connected with the circulating pump (5) through the pump suction pipe (4).
3. The atmospheric microbe on-line monitor of claim 2, wherein the multispectral detection system comprises: the system comprises a high-energy ultraviolet xenon lamp (7), a 15-bit narrowband filter rotating wheel component (9), a first condenser (10), a first plano-convex mirror (11), a 15-bit chopping filtering rotating wheel component (12), a second plano-convex mirror (13), an optical fiber connecting plate (14), a multimode optical fiber (15) and a multispectral detector (16);
the high-energy ultraviolet xenon lamp (7) and the 15-bit narrowband filter rotating wheel assembly (9) are positioned on one side of the gas collecting tube (3), and the 15-bit narrowband filter rotating wheel assembly (9) is positioned on the optical axis of the high-energy ultraviolet xenon lamp (7);
the first condenser lens (10), the first plano-convex lens (11), the 15-bit chopping filtering rotating wheel assembly (12), the second plano-convex lens (13) and the optical fiber connecting plate (14) are coaxially arranged on the other side of the gas collecting tube (3) in sequence and are arranged in the non-coaxial direction of the high-energy ultraviolet xenon lamp (7);
the optical fiber connecting plate (14) is connected with the multispectral detector (16) through the multimode optical fiber (15).
4. The atmospheric microbe on-line monitor as claimed in claim 3, wherein the optical axes of the first condenser (10) and the first plano-convex mirror (11) and the optical axis of the high-energy ultraviolet xenon lamp (7) are in the same plane and perpendicular to the central line of the gas collecting pipe (3).
5. The on-line atmospheric microbe monitor of claim 3, wherein the fluorescence lifetime detection system comprises: a high-frequency pulse laser (18), a high-energy optical filter (20), a third plano-convex mirror (21), a second condenser (22), a fourth plano-convex mirror (23), a long-wave transmission optical filter (24), a fifth plano-convex mirror (25) and a photomultiplier (26);
the high-frequency pulse laser (18), the high-energy optical filter (20) and the third plano-convex mirror (21) are positioned on one side of the gas collecting pipe (3);
the high-energy optical filter (20) and the third plano-convex mirror (21) are positioned in the emission direction of the emission optical axis of the high-frequency pulse laser (18), and the third plano-convex mirror (21) is focused at the central line position of the gas collecting pipe (3);
the second condenser (22), the fourth plano-convex lens (23), the long-wave transmission filter (24), the fifth plano-convex lens (25) and the photomultiplier (26) are coaxially arranged on the other side of the gas collecting tube (3) in sequence and are arranged in the non-coaxial direction of the emission optical axis of the high-frequency pulse laser (18);
the axis of the second condenser (22) and the axis of the high-frequency pulse laser (18) are in the same plane and are perpendicularly intersected with the central line of the gas collecting pipe (3).
6. The atmospheric microorganism on-line monitor according to claim 5, wherein the high-frequency pulse laser (18), the high-energy filter (20), the third plano-convex mirror (21), the high-energy ultraviolet xenon lamp (7) and the 15-bit narrowband filter rotating wheel assembly (9) are positioned on the same side of the gas collecting pipe (3) and are arranged in layers.
7. The online atmospheric microbe monitor of claim 5, wherein the high-speed data acquisition system comprises: the system comprises a high-energy ultraviolet xenon lamp power supply (6), a high-frequency pulse laser power supply (17), a filter disc rotating wheel driver (8), a time-dependent single photon counter (27), an embedded industrial personal computer (28) and online monitoring software (29);
the high-energy ultraviolet xenon lamp power supply (6) is connected with the high-energy ultraviolet xenon lamp (7), the filter rotating wheel driver (8) is respectively connected with the 15-bit narrowband filter rotating wheel component (9) and the 15-bit chopping filtering rotating wheel component (12), the high-frequency pulse laser power supply (17) is connected with the high-frequency pulse laser (18), the time-dependent single photon counter (27) is respectively connected with the high-frequency pulse laser power supply (17) and the photomultiplier (26), the embedded industrial personal computer (28) is respectively connected with the high-energy ultraviolet lamp power supply (6), the high-frequency pulse laser power supply (17), the filter rotating wheel driver (8), the time-dependent single photon counter (27) and the circulating pump (5), and the online monitoring software (29) is located on the embedded industrial personal computer (28).
8. The atmospheric microbe on-line monitor according to claim 7, wherein 15 narrow band filters of 250nm, 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm and 390nm are installed on the 15-position narrow band filter rotating wheel assembly (9) in turn anticlockwise;
15 long-wave pass filters of 260nm, 270nm, 280nm, 290nm, 300nm, 310nm, 320nm, 330nm, 340nm, 350nm, 360nm, 370nm, 380nm, 390nm and 400nm are sequentially arranged on the 15-bit chopping filtering rotating wheel component (12) in a counterclockwise manner.
9. The atmospheric microbe on-line monitor according to claim 8,
the narrow band filters on the 15-bit narrow band filter rotating wheel component (9) and the long-wave pass filters on the 15-bit chopping filtering rotating wheel component (12) sequentially correspond to each other according to a counterclockwise installation sequence;
and the online monitoring software (29) controls the filter rotating wheel driver (8) to drive the 15-bit narrow-band filter rotating wheel component (9) to rotate clockwise through the embedded industrial personal computer (28), and synchronously drives the 15-bit chopping filtering rotating wheel component (12) to rotate clockwise.
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