CN201477073U - Agarose gel DNA/RNA electrophoresis apparatus - Google Patents

Agarose gel DNA/RNA electrophoresis apparatus Download PDF

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Publication number
CN201477073U
CN201477073U CN2009201538872U CN200920153887U CN201477073U CN 201477073 U CN201477073 U CN 201477073U CN 2009201538872 U CN2009201538872 U CN 2009201538872U CN 200920153887 U CN200920153887 U CN 200920153887U CN 201477073 U CN201477073 U CN 201477073U
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gel
electrolytic bath
ago
platform
dna
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Expired - Fee Related
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CN2009201538872U
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Chinese (zh)
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康景轩
程浩然
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Individual
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Individual
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Abstract

The utility model relates to an agarose gel DNA/RNA electrophoresis apparatus, which comprises an electrolytic bath, wherein the wall of the electrolytic bath is made of a nontransparent material, a gel platform is arranged in the electrolytic bath, a pair of electrodes are arranged at the left end and the right end in the electrolytic bath, the electrodes are connected with electrode bars, an ultraviolet lamp chamber is arranged below the gel platform, at least one ultraviolet lamp tube with the wavelength of 305-315millimeters is arranged in the ultraviolet lamp chamber, the ultraviolet lamp tube is connected with an ultraviolet lamp power supply switch through a lead, a top plate of the ultraviolet lamp chamber simultaneously forms a plane for placing gel on the gel platform, and the top plate of the ultraviolet lamp chamber is made of a transparent material capable of permeating ultraviolet rays. Ultraviolet lamps are installed in the gel platform, so the electrophoretic separation process of DNA/RNA can be observed under the condition without transferring the gel.

Description

Ago-Gel DNA/RNA electrophoresis apparatus
Technical field
The utility model relates to a kind of electrophoresis apparatus, particularly a kind of Ago-Gel DNA/RNA electrophoresis apparatus.
Background technology
The DNA/RNA sample is experiment common in the Biochemical Lab, so the DNA/RNA electrophoresis apparatus is the essential instrument of each Biochemical Lab to analyze (or separation) by agarose gel electrophoresis.Use when having instrument now; the Ago-Gel that the experimenter needs DNA/RNA will be housed is delivered to the darkroom from the instrument location; put on the clothes of UV protection; observe or take DNA/RNA image in the gel with a kind of ultraviolet imagery device again; this experimentation is very consuming time; and need additional expensive equipment, for example: the darkroom.
The utility model content
The technical problems to be solved in the utility model provides a kind of Ago-Gel DNA/RNA electrophoresis apparatus, and cost is low and can observe DNA/RNA sample electrophoresis result quickly and easily.
For solving the problems of the technologies described above, the utility model adopts following technical scheme:
The utility model is a kind of Ago-Gel DNA/RNA electrophoresis apparatus, wherein, comprise electrolytic bath, the wall of electrolytic bath is made with lighttight material, in electrolytic bath, be provided with the gel platform, there is pair of electrodes at two ends, the left and right sides in the electrolytic bath, electrode links to each other with electrode bar, the below of gel platform is provided with ultraviolet lamp house, UV-lamp is indoor to be provided with the quartz burner that at least one wavelength is 305-315mm, quartz burner links to each other with the UV-lamp power switch by lead, and the top board of ultraviolet lamp house also constitutes be the plane of putting gel on the gel platform simultaneously, and the top board of ultraviolet lamp house is with making through ultraviolet transparent material.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, wherein, the two side of gel platform accompanies the anchor clamps that a cross section is roughly the X type, and the two clamping plate length of anchor clamps and the left and right sides wall of gel platform adapt, and are close on the wall of the left and right sides.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, wherein, electrolytic bath is provided with lighttight rectangle electrolytic bath top cover, two electrode connectors and an observation window are arranged on it, observation window be positioned at the gel platform directly over, observation window is provided with uvioresistant clear covering, and the position of two electrode connectors is corresponding with electrode bar.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model wherein, is installed an outer cover on the electrolytic bath top cover, video camera is housed in the outer cover, and the axle at the camera lens place of video camera is vertical with the plane at observation window place, and the center by observation window.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, wherein, the camera lens on the described video camera is the CCD camera lens, the focus adjustment button is installed on the outer wall of outer cover.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, wherein, the base plate of ultraviolet lamp house is made by reflectorized material.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, wherein, described video camera links to each other with computer.
Ago-Gel DNA/RNA electrophoresis apparatus described in the utility model, described UV-lamp power switch is on the outside surface of electrolytic bath, also be provided with timer on the outside surface of electrolytic bath, have at least an end to be provided with a pair of groove in the two ends, the left and right sides of gel platform, be used to lay the comb of preparation gel.
The utility model has been installed ultraviolet lamp house in electrolytic bath, can be under the situation that does not shift gel the detachment process of Direct observation and control DNA/RNA, need not the darkroom etc. expensive equipment, reduced cost.Simultaneously, the utility model has installed the electrolytic bath top cover additional on electrolytic bath, and coverture of observation window can filter ultraviolet ray on it, so, the observation personnel, need not to wear protective clothing just observe can be directly detachment process degree by naked-eye observation DNA/RNA.The utility model links to each other video camera and can realize at a distance the DNA/RNA detachment process being monitored with computer.Use anchor clamps, make that to make Ago-Gel on the gel platform convenient, again Ago-Gel is moved into the process of gel platform after avoiding making glue in other place.
Description of drawings
Fig. 1 is the structural representation of the utility model Ago-Gel DNA/RNA electrophoresis apparatus;
Fig. 2 is the partial sectional view of ultraviolet lamp house;
Fig. 3 is that anchor clamps are clipped in the synoptic diagram on the gel platform.
Embodiment
The utility model Ago-Gel DNA/RNA electrophoresis apparatus as depicted in figs. 1 and 2, comprise electrolytic bath 2, the wall of electrolytic bath 2 uses lighttight material to make, there is a gel platform 3 in central authorities in electrolytic bath 2, the left end 20 ' of gel platform 3 is provided with a pair of groove 19,19 ', two ends, the left and right sides are respectively equipped with pair of electrodes 4 ' in the electrolytic bath 2,4, electrode 4 ', 4 with electrode bar 1 ', 1 links to each other, ultraviolet ray lamp house 5 is positioned at the below of gel platform, the quartz burner 6 that it is 310mm that ultraviolet ray is provided with two wavelength in the lamp house 5, quartz burner 6 links to each other with UV-lamp power switch 13 by lead, the top board 7 of ultraviolet ray lamp house 5 also constitutes be the plane of putting gel on the gel platform 3 simultaneously, be used to be and put gel, top board 7 usefulness of ultraviolet lamp house 5 can see through ultraviolet transparent material and make.
Operation for convenience, UV-lamp power switch 13 is arranged on the outside surface of electrolytic bath 2, also is provided with timer 15 on the outside surface.
In order to prevent the infringement of ultraviolet ray to human body, electrolytic bath 2 is provided with lighttight rectangle electrolytic bath top cover 9, two electrode connectors 10,10 ' and observation window 11 are arranged above it, observation window 11 be positioned at gel platform 3 directly over, uvioresistant clear covering 22 is arranged on the observation window 11, two electrode connectors 10,10 ' position are corresponding with electrode bar 1,1 ', and electrode bar 1,1 ' passes electrode connector 10,10 ' respectively.
For carrying out video shooting in the process of distant surveillance electrophoresis with to electrophoresis process, one outer cover 18 is installed on the observation window 11, video camera 16 is equipped with in the bottom of outer cover 18, the axle at the camera lens place of video camera 16 is vertical with the plane at observation window 11 places, and the center by observation window, camera lens on the video camera is the CCD camera lens, and focus adjustment button 17 is installed on the outer cover 18, and video camera 16 links to each other with computer.
In order to strengthen the uitraviolet intensity in the ultraviolet lamp house 5, the base plate of ultraviolet lamp house 5 is made by reflectorized material.
On gel platform 3, make glue for convenient as shown in Figure 3, at the left and right sides of gel platform 3 wall 8 ', 8 cross section being arranged is the floating holder 21 of X type, the clamping plate 14 of anchor clamps 21 are rectangle, the left and right sides wall 8 ', 8 of its length and gel platform 3 adapts, and is close on the gel platform left and right sides wall 8 ', 8.
The course of work:
End groove 19,19 ' at gel platform 3 is gone up the insertion comb, anchor clamps 21 are clipped in left and right sides wall 8 ', 8 places of gel platform 3, gel is injected platform, treat that gel solidifies the back and takes out anchor clamps 21 and comb, in electrolytic tank, inject electrolyte solution to not having gel.Stay the pitting place to inject DNA on gel, energising beginning electrophoresis is opened timer 15 simultaneously, closes electrolytic bath top cover 9, uviol lamp power switch 13 can be opened when needs are observed.Just can with the naked eye directly observe the DNA fluoroscopic image by the uvioresistant clear covering 22 on the observation window 11.Start video camera 16, the switch 17 of focusing is focused and can be realized shooting to the DNA fluoroscopic image, and video camera 16 gained signals import computer into.
The utility model has been installed ultraviolet lamp house in electrolytic bath, can observe and control the detachment process of DNA/RNA under the situation that does not shift gel.Installed the electrolytic bath top cover on electrolytic bath additional, the uvioresistant clear covering on the observation window on it can filter ultraviolet ray, so, testing crew, need not to wear protective clothing just can be directly detachment process degree by naked-eye observation DNA/RNA.
For further convenient use, anchor clamps 21 are clipped in left and right sides wall 8 ', 8 places of gel platform 3, by gel is injected platform, can guarantee the thickness that gel forms, and make gel can not flow out the scope of gel platform, saved the consumption of gel, again Ago-Gel has been moved into the process of gel platform after having avoided simultaneously making glue in other place.The outside surface of electrolytic bath install timer 15 and power switch 13 make manipulate convenient, video camera 16 link to each other with computer make its can remote observation to the shooting of RNA fluoroscopic image, in order to increase ultraviolet light intensity, the end of ultraviolet lamp house 5, made by reflectorized material.

Claims (8)

1. Ago-Gel DNA/RNA electrophoresis apparatus, it is characterized in that, comprise electrolytic bath (2), the wall of electrolytic bath (2) is made with lighttight material, two ends, the interior left and right sides of electrolytic bath (2) (12 ', 12) pair of electrodes (4 ' is arranged, 4), electrode (4 ', 4) with electrode bar (1 ', 1) links to each other, in electrolytic bath (2), be provided with gel platform (3), the below of gel platform is provided with ultraviolet lamp house (5), be provided with the quartz burner that at least one wavelength is 305-315mm (6) in the ultraviolet ray lamp house (5), quartz burner (6) links to each other with UV-lamp power switch (13) by lead, the top board (7) of ultraviolet ray lamp house (5) also constitutes on the gel platform (3) simultaneously and is the plane of putting gel, and the top board (7) of ultraviolet lamp house (5) is with making through ultraviolet transparent material.
2. Ago-Gel DNA/RNA electrophoresis apparatus as claimed in claim 1, it is characterized in that, the two side (8) of gel platform (3), (8 ') accompany the anchor clamps (21) that a cross section is roughly the X type, the left and right sides wall (8 ', 8) of two clamping plate (14) length of anchor clamps (21) and gel platform (3) adapts, and is close on the left and right sides wall (8 ', 8).
3. Ago-Gel DNA/RNA electrophoresis apparatus as claimed in claim 2, it is characterized in that, electrolytic bath (2) is provided with lighttight rectangle electrolytic bath top cover (9), two electrode connectors (10 ', 10) and an observation window (11) are arranged on it, observation window (11) be positioned at the gel platform directly over, observation window (11) is provided with uvioresistant clear covering (22), and the position of two electrode connectors (10,10 ') is corresponding with electrode bar (1 ', 1).
4. Ago-Gel DNA/RNA electrophoresis apparatus as claimed in claim 3, it is characterized in that, electrolytic bath top cover (9) is gone up an outer cover (18) is installed, video camera (16) is housed in the outer cover (18), the axle at the camera lens place of video camera (16) is vertical with the plane at observation window (11) place, and the center by observation window.
5. Ago-Gel DNA/RNA electrophoresis apparatus as claimed in claim 4 is characterized in that the camera lens on the described video camera (16) is the CCD camera lens, and focus adjustment button (17) is installed on the outer wall of outer cover (18).
6. as 5 described Ago-Gel DNA/RNA electrophoresis apparatuses of claim, it is characterized in that the base plate of ultraviolet lamp house (5) is made by reflectorized material.
7. as 6 described Ago-Gel DNA/RNA electrophoresis apparatuses of claim, it is characterized in that described video camera links to each other with computer.
8. as 1 to 7 any described Ago-Gel DNA/RNA electrophoresis apparatus of claim, it is characterized in that, described UV-lamp power switch (13) is arranged on the outside surface of electrolytic bath (2), also be provided with timer (15) on the outside surface of electrolytic bath (2), have at least an end to be provided with a pair of groove (19,19 ') in the two ends, the left and right sides of gel platform (20 ', 20).
CN2009201538872U 2009-05-07 2009-05-07 Agarose gel DNA/RNA electrophoresis apparatus Expired - Fee Related CN201477073U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009201538872U CN201477073U (en) 2009-05-07 2009-05-07 Agarose gel DNA/RNA electrophoresis apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2009201538872U CN201477073U (en) 2009-05-07 2009-05-07 Agarose gel DNA/RNA electrophoresis apparatus

Publications (1)

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CN201477073U true CN201477073U (en) 2010-05-19

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102866194A (en) * 2012-09-05 2013-01-09 上海市东方医院 Multivariate electrophoresis interpretoscope
CN107340327A (en) * 2017-07-24 2017-11-10 西工大常熟研究院有限公司 A kind of gel electrophoresis groove with LED light source
CN108970402A (en) * 2018-07-16 2018-12-11 陈叶 A kind of medicine electrophoresis structure-improved
CN109085231A (en) * 2018-08-31 2018-12-25 广州市众为生物技术有限公司 A kind of new type gel electrophoresis apparatus

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102866194A (en) * 2012-09-05 2013-01-09 上海市东方医院 Multivariate electrophoresis interpretoscope
CN107340327A (en) * 2017-07-24 2017-11-10 西工大常熟研究院有限公司 A kind of gel electrophoresis groove with LED light source
CN108970402A (en) * 2018-07-16 2018-12-11 陈叶 A kind of medicine electrophoresis structure-improved
CN109085231A (en) * 2018-08-31 2018-12-25 广州市众为生物技术有限公司 A kind of new type gel electrophoresis apparatus

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C17 Cessation of patent right
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Granted publication date: 20100519

Termination date: 20110507