CN213726586U

CN213726586U - Batch urea feeding device for page process

Info

Publication number: CN213726586U
Application number: CN202021902233.8U
Authority: CN
Inventors: 李见朋; 苏敏
Original assignee: Shanghai Bioligo Biotechnology Co ltd
Current assignee: Briggs Biotechnology Shanghai Co ltd
Priority date: 2020-09-03
Filing date: 2020-09-03
Publication date: 2021-07-20
Anticipated expiration: 2030-09-03

Abstract

The utility model relates to a DNA correlation technique auxiliary assembly field specifically discloses a add urea device in batches for page technology, including first box, first box below is equipped with first baffle, first baffle below is equipped with the second baffle, first baffle with be equipped with quantitative urea pipeline between the second baffle, second baffle below is equipped with the second box. The utility model discloses can add urea in batches, the application of sample has 24 or 36 hole sites at every turn, very big saving time and human cost.

Description

Batch urea feeding device for page process

Technical Field

The utility model relates to a DNA correlation technique auxiliary assembly field specifically is a batch adds urea device for page technology.

Background

The principle of gel electrophoresis is relatively simple. When a molecule is placed in an electric field, it moves to the appropriate electrode at a certain speed, and the migration speed of the electrophoretic molecule under the action of the electric field is called electrophoretic mobility. Since the electrophoretic mobility is inversely proportional to the friction coefficient of the molecule due to the reduced convective motion of the electrophoretic support medium, such as agarose gel and polyacrylamide gel, which are unreactive and stable. It is known that the coefficient of friction is a function of the size, polarity and viscosity of the medium of the molecule, and thus, depending on the size, composition or shape of the molecule, and the amount of net charge carried, the components of a mixture of protein or nucleic acid molecules can be separated from each other by electrophoresis. At a certain electric field strength, the migration speed of DNA molecules, i.e., the electrophoretic mobility, depends on the size and configuration of the nucleic acid molecules themselves, and DNA molecules with smaller molecular weight migrate faster than DNA molecules with larger molecular weight. This is the basic principle for the separation of DNA fragments by gel electrophoresis.

Usually, most of the methods of DNA electrophoresis are agarose gel electrophoresis. In addition to agarose gel, PAGE can be used to run DNA electrophoresis; in 1984, Pulsed-field gel electrophoresis (PFGE) techniques invented by d.c. schwartz and c.r. cantor were successfully used to separate DNA molecules with ultra-large molecular weight such as whole chromosomes.

In standard PFGE, the direction of the electric field of the first pulse is at a 45 ° angle to the direction of nucleic acid movement, while the direction of the electric field of the next pulse is at a 45 ° angle to the direction of nucleic acid movement on the other side. Because the direction of the electric field, the magnitude of the current and the acting time of the agarose gel are alternately changed, the DNA molecules can adjust the swimming direction at any time to adapt to the random change of the gel pores. A larger molecular weight DNA molecule requires more times to change its configuration and orientation than a smaller molecular weight DNA molecule, so that it can swim in a new direction. Therefore, the migration rate in the agarose medium is slower, so as to achieve the purpose of separating the DNA molecules with ultra-large molecular weight. By applying the pulsed electric field gel electrophoresis technology, DNA macromolecules with the molecular weight up to 10bp can be successfully separated.

The DNA urea-PAGE sample solution is a very common sample solution in the PAGE process, and has the advantages of being ready to use and not needing to prepare various solutions independently. Simple to use, the DNA sample electrophoresed was mixed with the sample loading solution 1: 1, mixing, heating to 95 ℃ for 5 minutes, and cooling at 0 ℃ to directly load the sample. The dye has small molecules, and the observation of DNA bands can not be interfered during dyeing. Compatible with the DNA urea-PAGE denaturing electrophoresis suit of the company; however, in the process, the application of the DNA urea-PAGE sample solution is independently and manually operated, so that the efficiency is very low, and a large amount of labor and time cost is wasted.

Disclosure of Invention

An object of the utility model is to provide a add urea device in batches for page technology to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides a add urea device in batches for page technology, includes first box, first box below is equipped with first baffle, first baffle below is equipped with the second baffle, first baffle with be equipped with quantitative urea pipeline between the second baffle, second baffle below is equipped with the second box.

As a further aspect of the present invention: the first partition board is provided with an object guiding hole, and the second partition board is provided with an object guiding hole.

As a further aspect of the present invention: and the two ends of the quantitative urea pipeline are matched with the primer holes.

As a further aspect of the present invention: the number of the object guiding holes in the first partition plate and the number of the object guiding holes in the second partition plate are 24, and the number of the quantitative urea pipelines is 24.

As a further aspect of the present invention: the number of the guiding holes on the first partition plate and the second partition plate is 36, and the number of the quantitative urea pipelines is 36.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses can add urea in batches, the application of sample has 24 or 36 hole sites at every turn, very big saving time and human cost.

Drawings

In order to illustrate the technical solutions in the embodiments of the present invention more clearly, the drawings that are needed in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention.

FIG. 1 is a schematic diagram of a batch urea addition apparatus for use in a page process.

In the figure: 1-a first box body, 2-a first clapboard, 3-a quantitative urea pipeline, 4-a second clapboard, 5-a second box body and 6-an object guide hole.

Detailed Description

In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1

Referring to fig. 1, in an embodiment of the present invention, a batch urea feeding device for a page process includes a first box 1, a first partition plate 2 is disposed below the first box 1, an introducing hole 6 is formed in the first partition plate 2, a second partition plate 4 is disposed below the first partition plate 2, an introducing hole 6 is formed in the second partition plate 4, a quantitative urea pipeline 3 is disposed between the first partition plate 2 and the second partition plate 4, and two ends of the quantitative urea pipeline 3 are matched with the introducing hole 6; a second box body 5 is arranged below the second partition plate 4;

furthermore, there are 24 guiding holes 6 on the first partition board 2 and the second partition board 4, and correspondingly, there are 24 quantitative urea pipelines 3.

The embodiment of the utility model provides a theory of operation is: the method comprises the steps of firstly placing a primer plate for transferring urea to be added at the bottom of the device, closing a first partition plate 2 and a second partition plate 4, placing dried urea particles into a first box body 1, opening the first partition plate 2 to enable the urea to uniformly flow into quantitative urea pipelines 3, ensuring that each quantitative urea pipeline 3 is filled with the urea, closing the first partition plate 2, opening the second partition plate 4 to enable the urea in the quantitative urea pipelines 3 to flow into corresponding lead holes, and taking out the primer plate added with the urea from a second box body 5.

Example 2

furthermore, there are 36 inlet holes 6 on the first partition board 2 and the second partition board 4, and correspondingly, there are 36 dosing urea pipes 3.

The embodiment of the utility model provides a theory of operation is: unlike example 1, in this example, there are 36 inlet holes 6 and dosing urea pipes 3, and compared to example 1, the scale is enlarged and the efficiency is improved.

In the description of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims

1. The utility model provides a add urea device in batches for page technology, a serial communication port, includes first box (1), first box (1) below is equipped with first baffle (2), first baffle (2) below is equipped with second baffle (4), first baffle (2) with be equipped with quantitative urea pipeline (3) between second baffle (4), second baffle (4) below is equipped with second box (5).

2. The batch urea feeding device for the page process according to claim 1, wherein the first partition plate (2) is provided with an inlet hole (6), and the second partition plate (4) is provided with an inlet hole (6).

3. The batch urea feeding device for a page process according to claim 2, wherein both ends of the quantitative urea pipeline (3) are matched with the guiding holes (6).

4. The batch urea feeding device for the page process according to any one of the claims 1 to 3, wherein the number of the introducing holes (6) on the first partition plate (2) and the second partition plate (4) is 24, and the number of the quantitative urea pipes (3) is 24.

5. The batch urea feeding device for the page process according to any one of the claims 1 to 3, wherein the number of the introducing holes (6) on the first partition plate (2) and the second partition plate (4) is 36, and the number of the quantitative urea pipes (3) is 36.