CN203658307U - Real-time visible and image-sharing nucleic acid electrophoresis device - Google Patents
Real-time visible and image-sharing nucleic acid electrophoresis device Download PDFInfo
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- CN203658307U CN203658307U CN201320888634.6U CN201320888634U CN203658307U CN 203658307 U CN203658307 U CN 203658307U CN 201320888634 U CN201320888634 U CN 201320888634U CN 203658307 U CN203658307 U CN 203658307U
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- imaging
- nucleic acid
- acid electrophoresis
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- led excitation
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- NOIIUHRQUVNIDD-UHFFFAOYSA-N 3-[[oxo(pyridin-4-yl)methyl]hydrazo]-N-(phenylmethyl)propanamide Chemical compound C=1C=CC=CC=1CNC(=O)CCNNC(=O)C1=CC=NC=C1 NOIIUHRQUVNIDD-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 238000003384 imaging method Methods 0.000 claims abstract description 101
- 230000005284 excitation Effects 0.000 claims abstract description 40
- 230000003287 optical effect Effects 0.000 claims description 32
- 238000001962 electrophoresis Methods 0.000 claims description 21
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 8
- 230000005540 biological transmission Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 108020004707 nucleic acids Proteins 0.000 description 20
- 150000007523 nucleic acids Chemical class 0.000 description 20
- 102000039446 nucleic acids Human genes 0.000 description 20
- 239000000980 acid dye Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 230000009182 swimming Effects 0.000 description 3
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
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- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000031709 bromination Effects 0.000 description 1
- 238000005893 bromination reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005685 electric field effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- -1 sypro green Chemical compound 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a real-time visible and image-sharing nucleic acid electrophoresis device which comprises a device base, a set-top cover, a nucleic acid electrophoresis device, an imaging LED (Light-Emitting Diode) excitation light source, an imaging light source filter, observation filter plates, a lens filter and an imaging lens, wherein the imaging LED excitation light source is arranged on the lower surface of the set-top cover, the imaging light source filter is arranged between the imaging LED excitation light source and a nucleic acid electrophoresis bath and between the observation filter plates; the imaging lens is arranged below the nucleic acid electrophoresis bath, and the imaging lens filter is arranged above the imaging lens; the centers of the imaging LED excitation light source, the imaging light source filter, observation filter plates, the lens filter and the imaging lens are in the same one axis. The real-time visible and image-sharing nucleic acid electrophoresis device can be used for effectively combining fluorescent nucleic acid electrophoresis, instant imaging, image wireless transmission, global data sharing and other advanced technologies and filling a blank of the national market, and can be widely applied to the fields of biology, medicals, agricultures and the like needing molecular biology experiments.
Description
Technical field
The utility model relates to a kind of nucleic acid electrophoresis appts, relates to more specifically the shared nucleic acid electrophoresis appts of a kind of Real time visible and imaging, belongs to molecular biology experiment field.
Background technology
The gel electrophoresis experiment of DNA and RNA is at the normal experiment in biological study and medical research field one, no matter be the checking of extracting in plasmid, the displaying of PCR result, or gene order-checking early stage sample preliminary work recruitment evaluation, the gel electrophoresis of nucleic acid is all to provide nucleic acid purity for experimenter, concentration, the effective method of the information such as clip size, along with the development of biological medicine technology, for ease for use and the wide usage of DNA and RNA gel electrophoresis, more and more higher demand is proposed.
Now widely used nucleic acid developing technique be mainly by ultraviolet source to being embedded in exciting of bromination pyridine (EB) in sample of nucleic acid, observe the position of nucleic acid swimming in gel and reclaim nucleic acid.Mainly there is following defect in current technology: (1) EB is that height causes toxicant, there is very strong mutagenesis, easily cause cell generation canceration, and high volatility is difficult to again degraded, require to use the laboratory of EB to set up special EB contaminated area, the experimenter who simultaneously carries out nucleic acid electrophoresis and experiment need carry out very careful protection, otherwise will produce harm to the even whole laboratory of experimenter; (2) ultraviolet source possesses the harmfulness of DNA damage equally, easily experimenter is produced to damage; (3) EB develops needs the specialized equipment of ultraviolet imagery, and not only price is higher but also make technically electrophoresis process separate with developing process, has reduced the controllability of electrophoresis experiment, has wasted valuable experimental period.In recent years, countries in the world are all in the possibility of being devoted to study EB substitute, as genefinder, the fluorescent core acid dyes such as sypro green, these fluorescent core acid dyes are ensureing under the prerequisite of sensitivity, adopt visible light source fluorescence excitation nucleic acid dye, produce visible detection light source, reach the object that detects nucleic acid.The appearance of these fluorescent core acid dyes, to having proposed active demand taking nontoxic low toxicity fluorescent core acid dye as the research and development of basic fluorescence nucleic acid electrophoresis equipment, at present, on market, existing nucleic acid electrophoresis equipment, has greatly limited its use owing to not reusing.
In addition, user needs the image result of permanent reservation nucleic acid electrophoresis, generally needs the gel imaging equipment of an other platform independent to complete.When operation, the gel that contains nucleic acid need to be transferred to gel imaging equipment from nucleic acid electrophoresis equipment, just can be completed imaging process; And the general computing machine that also needs an other platform independent, carries out storage operation to the gel images of taking; Finally, the image in computing machine is downloaded by memory elements such as USB flash disks, then uploaded in terminal user's computing machine, complex operation, workload is large.
Utility model content
Technical problem to be solved in the utility model is, overcomes the shortcoming of prior art, a kind of real time imagery, Real time visible is provided, can carries out reclaimer operation and the remote shared nucleic acid electrophoresis appts of imaging.
In order to solve above technical matters, the utility model provides a kind of Real time visible and the shared nucleic acid electrophoresis appts of imaging, comprise device pedestal, machine cap and nucleic acid electrophoresis appts, described device pedestal is the box body of a cavity, described nucleic acid electrophoresis appts is arranged at the top of described base, and described machine cap is arranged at described nucleic acid electrophoresis appts top; Described nucleic acid electrophoresis appts comprises electrophoresis tank and observes filter, described observation filter is arranged at the top of described electrophoresis tank, also comprise imaging LED excitation source, imaging source optical filter, camera lens optical filter and imaging lens, described imaging LED excitation source is arranged at the lower surface of described machine cap, and described imaging source optical filter is arranged between described imaging LED excitation source and described electrophoresis tank; Described imaging lens is arranged at the bottom of described device pedestal, and described camera lens optical filter is arranged at the top of described imaging lens; The center of described imaging LED excitation source, imaging source optical filter, observation filter, electrophoresis tank, camera lens optical filter and imaging lens is on same axis.Observing filter is orange optical filter, guarantees to only have the fluorescence of nucleic acid to see through, and reaches the object of Real Time Observation nucleic acid electrophoresis with this; And in order to ensure the unicity of light source, imaging source optical filter is blue color filter, make the nucleic acid electrophoresis can blur-free imaging.Observe and test through experiment, each light source and optical filter are arranged on the axis of electrophoresis tank, imaging LED excitation source, imaging source optical filter and camera lens optical filter and imaging lens are used in conjunction with each other, and can make the imaging of nucleic acid electrophoresis more clear, use convenient.
Being further defined to of technical solutions of the utility model, also comprise central processing unit and touch display screen, described central processing unit is connected with described imaging lens and touch display screen data, receives the image that imaging lens gathers, and by extremely described touch display screen of described image transmitting.
Further, also comprise and the wireless launcher of described central processing unit communication connection, described wireless launcher is Wifi, bluetooth or 3G.
Further, described imaging lens is CCD imaging lens or cmos imaging camera lens.
Further, also comprise side LED excitation source and side light source optical filter, described side LED excitation source is arranged at the side of described device pedestal, and described side light source optical filter is arranged at the front end of described LED excitation source.Side LED excitation source and side light source optical filter have been avoided the axis of imaging source and imaging lens, although side light source optical filter is just not right with electrophoresis tank, but can provide for electrophoresis tank amplifying nucleic acid fluorescent dye the light source of sufficient intensity, having reduced intense light source may be to the stimulation of eyes, is convenient to observe and reclaim.
Further, described imaging LED excitation source is connected with different power switches from described side LED excitation source.
The beneficial effects of the utility model are: a kind of real time imagery, Real time visible that the utility model provides, can carry out reclaimer operation and the remote shared nucleic acid electrophoresis appts of imaging, when imaging, make the fluorescent dye of being combined with nucleic acid luminous by top LED matrix excitation source, see through imaging filter and be inverted taking lens the real time imagery object that reaches nucleic acid electrophoresis; When observing and reclaiming, make the fluorescent dye of being combined with nucleic acid luminous by side LED matrix excitation source, see through and observe filter and reach the object that Real Time Observation and nucleic acid reclaim; By built-in W iFi or bluetooth or 3G function, use the modern network means such as cloud storage of micro-letter group or QQ group or image/video to reach the wireless transmission of electrophoretic image result and the object of multi-user whole world Instantaneous sharing.The utility model adopts two light source Real Time Observation and reclaims fluorescence nucleic acid electrophoresis, fluorescence nucleic acid electrophoresis is inverted into picture, share in the instant nucleic acid electrophoresis result whole world, effectively combine fluorescence nucleic acid electrophoresis, instant imaging, image wireless transmission and the data whole world and the advanced technology such as share, fill up home market blank, can be widely used in biology, medicine, agricultural etc. need to be carried out the field of molecular biology experiment.
Brief description of the drawings
Fig. 1 is the structural representation of the shared nucleic acid electrophoresis appts of a kind of Real time visible of the present utility model and imaging.
Embodiment
A kind of Real time visible that the present embodiment provides and the shared nucleic acid electrophoresis appts of imaging, its structural representation as shown in Figure 1, comprises device pedestal 11 and nucleic acid electrophoresis appts.
Nucleic acid electrophoresis appts is arranged at the top of described base 11, is the miniature nucleic acid electrophoresis appts of insertion slot type, comprises electrophoresis tank 6 and observes filter 4, and observation filter 4 is arranged at the top of electrophoresis tank 6.Miniature nucleic acid electrophoresis appts can adopt the conventional nucleic acid electrophoresis appts in this area, in its internal feature the present embodiment of its structure, repeats no more.
The shared nucleic acid electrophoresis appts of the Real time visible of the present embodiment and imaging also comprises real time imagery part, Real Time Observation part and imaging shared portion, will this three part be done to the introduction of emphasis below.
Real time imagery part comprises imaging LED excitation source 2, imaging source optical filter 3, camera lens optical filter 9 and imaging lens 10.Imaging LED excitation source 2 is arranged at the lower surface of machine cap 5, imaging source optical filter 3 be arranged at imaging LED excitation source 2 and and described electrophoresis tank 6 between, described observation filter 4 is on described electrophoresis tank 6.Imaging lens 10 is arranged at the bottom of device pedestal 11, and camera lens optical filter 9 is arranged at the top of imaging lens 10; Imaging LED excitation source 2, imaging source optical filter 3, camera lens optical filter 9 and imaging lens 10 center are on same axis.Imaging lens 10 is CCD imaging lens or cmos imaging camera lens.
Real Time Observation and recovery section comprise side LED excitation source 8 and side light source optical filter 7, and observation LED excitation source is arranged at the side of described device pedestal 11, and side light source optical filter 7 is arranged at the front end of described side LED excitation source 8.
Imaging shared portion comprises central processing unit and touch display screen 1, and central processing unit is connected with imaging lens 10 and touch display screen 1 data, receives the image that imaging lens 10 gathers, and by extremely described touch display screen 1 of described image transmitting.Touch display screen 1 is arranged at the top of base 11, is convenient to observe and use.
Meanwhile, the wireless launcher that imaging shared portion also comprises and described central processing unit communicates to connect, described wireless launcher is Wifi, bluetooth or 3G.Use the modern network means such as cloud storage of micro-letter group or QQ group or image/video to reach the wireless transmission of electrophoresis result and the object of multi-user whole world Instantaneous sharing.
In addition, imaging LED excitation source 2 is connected with different power switches from side LED excitation source 8, and it is controlled separately, easy to use.
The method of operating of the shared nucleic acid electrophoresis appts of a kind of Real time visible of the present embodiment and imaging is:
(1) on request fluorescent core acid dye (as sypro green etc.) is added in the gel of fusing in the time preparing gel, the Ago-Gel that solidifies preparing is placed in electrophoresis tank and adds a small amount of electrophoresis liquid, the nucleic acid that adds needs to analyze in gel pore.
(2), in the time that machine cap 5 is opened, this device is in observing and recovery state.Now, imaging LED excitation source 2 and imaging lens 10 are in closed condition, and side LED excitation source 8 is in opening.The light source of side LED light source 8 is through side light source optical filter 7, excite the fluorescent core acid dye of being combined with nucleic acid in electrophoresis tank 6 to send fluorescence, see through and observe filter 4, user can directly observe nucleic acid and under stationary electric field effect, do directed swimming, reaches the object of Real Time Observation nucleic acid electrophoresis.Lift and observe filter 4, user can carry out cutting operation to the nucleic acid in gel, reaches the object that reclaims target nucleic acid.
(3) in the time taking away observation filter 4 and close machine cap 5, this device is in imaging, data transmission and shared state.Now, side LED excitation source 8 is in closed condition, and imaging LED excitation source 2 and imaging lens 10 are in opening.User can by touch display screen 1 to nucleic acid the directed swimming in stationary electric field observe, take pictures, and can use at any time the modern network means such as cloud storage of micro-letter group or QQ group or image/video, with co-worker, Project Unit, customer group, tutor or related personnel share nucleic acid electrophoresis result in the world.
In addition to the implementation, the utility model can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of the utility model requirement.
Claims (6)
1. a Real time visible and the shared nucleic acid electrophoresis appts of imaging, comprise device pedestal (11), machine cap (5) and nucleic acid electrophoresis appts, described device pedestal (11) is the box body of a cavity, described nucleic acid electrophoresis appts is arranged at the top of described base (11), and described machine cap (5) is arranged at described nucleic acid electrophoresis appts top; Described nucleic acid electrophoresis appts comprises electrophoresis tank (6) and observes filter (4), described observation filter (4) is arranged at the top of described electrophoresis tank (6), it is characterized in that, also comprise imaging LED excitation source (2), imaging source optical filter (3), camera lens optical filter (9) and imaging lens (10), described imaging LED excitation source (2) is arranged at the lower surface of described machine cap (5), and described imaging source optical filter (3) is arranged between described imaging LED excitation source (2) and described electrophoresis tank (6); Described imaging lens (10) is arranged at the bottom of described device pedestal (11), and described camera lens optical filter (9) is arranged at the top of described imaging lens (10); The center of described imaging LED excitation source (2), imaging source optical filter (3), observation filter (4), electrophoresis tank (6), camera lens optical filter (9) and imaging lens (10) is on same axis.
2. a kind of Real time visible according to claim 1 and the shared nucleic acid electrophoresis appts of imaging, it is characterized in that, also comprise central processing unit and touch display screen (1), described central processing unit is connected with described imaging lens (10) and touch display screen (1) data, receive the image that imaging lens (10) gathers, and by extremely described touch display screen (1) of described image transmitting.
3. a kind of Real time visible according to claim 2 and the shared nucleic acid electrophoresis appts of imaging, is characterized in that, also comprises and the wireless launcher of described central processing unit communication connection, and described wireless launcher is Wifi, bluetooth or 3G.
4. a kind of Real time visible according to claim 1 and the shared nucleic acid electrophoresis appts of imaging, is characterized in that, described imaging lens (10) is CCD imaging lens or cmos imaging camera lens.
5. a kind of Real time visible according to claim 1 and the shared nucleic acid electrophoresis appts of imaging, it is characterized in that, also comprise side LED excitation source (8) and side light source optical filter (7), described side LED excitation source is arranged at the side of described device pedestal (11), and described side light source optical filter (7) is arranged at the front end of described side LED excitation source (8).
6. a kind of Real time visible according to claim 5 and the shared nucleic acid electrophoresis appts of imaging, is characterized in that, described imaging LED excitation source (2) is connected from different power switches with described side LED excitation source (8).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320888634.6U CN203658307U (en) | 2013-12-31 | 2013-12-31 | Real-time visible and image-sharing nucleic acid electrophoresis device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320888634.6U CN203658307U (en) | 2013-12-31 | 2013-12-31 | Real-time visible and image-sharing nucleic acid electrophoresis device |
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| CN203658307U true CN203658307U (en) | 2014-06-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103713033A (en) * | 2013-12-31 | 2014-04-09 | 南京山诺生物科技有限公司 | Real-time, visible and image-shared nucleic acid electrophoresis device |
| CN112285189A (en) * | 2020-09-28 | 2021-01-29 | 上海天能生命科学有限公司 | Method for remotely controlling electrophoresis apparatus based on image recognition |
-
2013
- 2013-12-31 CN CN201320888634.6U patent/CN203658307U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103713033A (en) * | 2013-12-31 | 2014-04-09 | 南京山诺生物科技有限公司 | Real-time, visible and image-shared nucleic acid electrophoresis device |
| CN112285189A (en) * | 2020-09-28 | 2021-01-29 | 上海天能生命科学有限公司 | Method for remotely controlling electrophoresis apparatus based on image recognition |
| CN112285189B (en) * | 2020-09-28 | 2021-06-25 | 上海天能生命科学有限公司 | Method for remotely controlling electrophoresis apparatus based on image recognition |
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