CN206591103U - A kind of biochip of achievable PhastGel electrophoresis - Google Patents
A kind of biochip of achievable PhastGel electrophoresis Download PDFInfo
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- CN206591103U CN206591103U CN201720010609.6U CN201720010609U CN206591103U CN 206591103 U CN206591103 U CN 206591103U CN 201720010609 U CN201720010609 U CN 201720010609U CN 206591103 U CN206591103 U CN 206591103U
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- Prior art keywords
- electrophoresis
- biochip
- groove
- electrode
- gel
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 27
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 23
- 238000001502 gel electrophoresis Methods 0.000 claims abstract description 18
- 230000005611 electricity Effects 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005096 rolling process Methods 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 10
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000011810 insulating material Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 229910010271 silicon carbide Inorganic materials 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 12
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 238000003491 array Methods 0.000 abstract description 2
- 239000000872 buffer Substances 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 238000003745 diagnosis Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 229920000936 Agarose Polymers 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010147 laser engraving Methods 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 238000010146 3D printing Methods 0.000 description 1
- 239000004831 Hot glue Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000001259 photo etching Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model is related to a kind of biochip of achievable PhastGel electrophoresis, there is a rule plate gel electrophoresis groove of array arrangement on biochip, groove bottom water white transparency, there is the circular electrode groove that connection is communicated at plate gel electrophoresis groove two ends, positive and negative electrode can be respectively put into the middle of two end electrodes groove, electrode passes through all circular electrode grooves, gel is filled with plate gel electrophoresis electrophoresis tank, sample holes are formed rolling hole on the extreme gel of negative electricity, tbe buffer liquid is added in slot electrode, afterwards by DNA hand-holes bottom, positive and negative electrode, which is powered, carries out gel electrophoresis.By miniaturized arrays, so as to simplify operation, reaction and detection efficiency are improved.Hardware acquisition cost is reduced, artificial operation is simplified, improves efficiency.Suitable for DNA or protein PhastGel electrophoresis detection, to realize the diagnosis of clinical medicine disease and the detection and analysis of biomolecule.
Description
Technical field
The utility model is related to a kind of biochemistry detection equipment, more particularly to a kind of biological core of achievable PhastGel electrophoresis
Piece.
Background technology
With specific gene order（Fragment）Biological or tissue detection and identification are carried out as detection target, it has also become raw
Important technical in the fields such as thing, biochemistry, medical science, environment and food, is widely used.Conventional gel electricity
Swimming detection is present（1）It is long the time required to preparing gel；（2）Chemical reagent consumption is big；（3）Larger reaction volume adds biology
The consumption of reagent, the shortcomings of improving experimental cost.Due to more than operating process and comparatively laborious, this is not only to the hard of laboratory
Part condition and the operation level of experimenter are proposed the high requirement of comparison, and add operational error and sample pollution
Possibility.
The content of the invention
The utility model is to be directed to the problem of conventional gel electrophoresis detection is cumbersome, it is proposed that a kind of achievable PhastGel electricity
The biochip of swimming, be it is a kind of realize miniaturization it is simple in construction, cost is low, chip simple to operate, can be achieved PhastGel
The biochip of electrophoresis, can be used for quickly detecting to DNA.
The technical solution of the utility model is：A kind of biochip of achievable PhastGel electrophoresis, on biochip
There is a rule plate gel electrophoresis groove of array arrangement, bottom land water white transparency, plate gel electrophoresis groove two ends have what connection was communicated
Circular electrode groove, there is positive and negative electrode respectively in the middle of two end electrodes groove, and electrode passes through all circular electrode grooves, plate gel electrophoresis
Gel is filled with groove, sample holes are formed rolling hole on the extreme gel of negative electricity, sample holes are used to inject DNA reaction solutions.
The optional inorganic insulating material of material, organic insulation, insulating polymeric material, the macromolecule of the biochip
One kind in polymer, composite or combined material.
One kind in the material of the biochip in preferred acrylic, glass, quartz, silicon, carborundum.
The gel is the gel being configured to agarose or polyacrylamide.
The beneficial effects of the utility model are：The utility model can realize the biochip of PhastGel electrophoresis, with biography
System gel electrophoresis apparatus and detection method are compared, by by device miniaturized arrays, so as to simplify operation, improving reaction
And detection efficiency.This biochip realizes miniaturization, reduces the quantity of equipment, reduces hardware acquisition cost, simplifies
Artificial operation, improves efficiency.Suitable for DNA or protein PhastGel electrophoresis detection, to realize examining for clinical medicine disease
The detection and analysis of disconnected and biomolecule.
Brief description of the drawings
Fig. 1 is that the utility model can realize the biochip structural representation of PhastGel electrophoresis；
Fig. 2 is that the utility model can realize the biochip matching sample holes comb structure schematic diagram of PhastGel electrophoresis；
Fig. 3 is the experimental result picture one that the utility model 50bp DNA ladder carry out electrophoresis in Ago-Gel；
Fig. 4 is the experimental result picture two that the utility model 100bp DNA ladder carry out electrophoresis in Ago-Gel.
Embodiment
The biochip structural representation of achievable PhastGel electrophoresis, uses engraving or notes on chip as shown in Figure 1
One rule plate gel electrophoresis groove 2 of molding technology array arrangement, bottom land water white transparency, if plate gel electrophoresis groove 2 is cut through, just
Sealed in chip back with biogum paster, there is the circular electrode groove 1 that connection is communicated at the two ends of plate gel electrophoresis groove 2,
There is positive and negative electrode 3 and 4 in the middle of two end electrodes groove 1 respectively, electrode passes through all circular electrode grooves 1, plate gel electrophoresis electrophoresis tank 2
Interior injection agarose hot glue solution, cools down gel afterwards, and empty formation sample holes are being rolled on the gel at the end of negative electrode 4
5, slowly decant(-ation) enters in sample holes 5 DNA sample pipettor, and tbe buffer liquid is added in slot electrode 1 to maintain suitable pH simultaneously
And make solution that there is certain electric conductivity, in favor of the migration of DNA molecular, positive and negative electrode connects power supply, turns on the power, and carries out
Gel electrophoresis.
Required chip and to carve out respective shapes, i.e. multiple electrodes groove 1 and electrophoresis tank 2 on chip with laser engraving machine logical
Road, makes positive and negative electrode 3,4 may be inserted into wherein, is sealed in chip back with biogum paster.The material bag of the chip
Inorganic insulating material, organic insulation, insulating polymeric material, composite, high molecular polymer or combined material are included, it is excellent
Select acrylic, glass, quartz, silicon, carborundum.The gel electrophoresis chip by laser-engraving technique, micro electro mechanical processing technology,
One or more kinds of combination techniques in screen printing technique, 3D printing techniques, photoetching technique are made.
Gel is configured with agarose or polyacrylamide etc., is separately added into slot electrode 1 and electrophoresis tank 2, stands, treats it
Solidification.On gel in electrophoresis tank sample holes 5 are pricked close to one end of negative pole.PhastGel electrophoresis can be achieved as shown in Figure 2
Biochip matches sample holes comb structure schematic diagram.
Example：With the biochip and its relevant apparatus of a kind of achievable PhastGel electrophoresis to 100bp DNA
Ladder is detected.
50bp or 100bp DNA ladder, SYBR GREEN, 6X Loading Buffer are matched somebody with somebody according to a certain percentage
Put and obtain solution to be detected.0.5X TBE are added into beaker, weigh agarose powder and being mixed with reach 1~2% it
Between a certain concentration, beaker is sealed up into preservative film, several holes are pricked, heated 1 minute in micro-wave oven, after being taken out from micro-wave oven,
Solution is put into the electrophoresis tank on chip with pipettor immediately, after after its solidification, positive and negative electrode is inserted in slot electrode respectively,
Hole is pricked close to one end of negative pole on gel in electrophoresis tank, or directly using the chip for being inoculated with pre-prepared colloid, tears its sealing
Film, TBE solution is added into slot electrode, DNA solution to be measured is slowly added into sample holes, electrophoresis apparatus supply voltage is set to
180V, is turned on the power switch, and it is reacted 6~7 minutes, after reaction terminates, under dark room conditions, is carried out with fluoroscopic examination module
Observation.If Fig. 3 and 4 is experimental result picture.
Claims (3)
1. a kind of biochip of achievable PhastGel electrophoresis, it is characterised in that have the one of array arrangement on biochip
There is the circular electrode groove that connection is communicated at rule plate gel electrophoresis groove, bottom land water white transparency, plate gel electrophoresis groove two ends, two
There is positive and negative electrode in the middle of termination electrode groove respectively, electrode is filled with gel by all circular electrode grooves in plate gel electrophoresis groove,
Sample holes are formed rolling hole on the extreme gel of negative electricity, sample holes are used to inject DNA reaction solutions.
2. the biochip of PhastGel electrophoresis can be realized according to claim 1, it is characterised in that the biochip
One in the optional inorganic insulating material of material, organic insulation, insulating polymeric material, high molecular polymer, composite
Kind.
3. the biochip of PhastGel electrophoresis can be realized according to claim 2, it is characterised in that the biochip
One kind in material in preferred acrylic, glass, quartz, silicon, carborundum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720010609.6U CN206591103U (en) | 2017-01-05 | 2017-01-05 | A kind of biochip of achievable PhastGel electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720010609.6U CN206591103U (en) | 2017-01-05 | 2017-01-05 | A kind of biochip of achievable PhastGel electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206591103U true CN206591103U (en) | 2017-10-27 |
Family
ID=60127977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720010609.6U Expired - Fee Related CN206591103U (en) | 2017-01-05 | 2017-01-05 | A kind of biochip of achievable PhastGel electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206591103U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
| CN111812091A (en) * | 2020-06-28 | 2020-10-23 | 上海交通大学 | Chip gel electrophoresis and on-line UV-VIS imaging detection device thereof |
| CN114324543A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Electrode and use thereof |
| CN114317238A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
-
2017
- 2017-01-05 CN CN201720010609.6U patent/CN206591103U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
| CN111812091A (en) * | 2020-06-28 | 2020-10-23 | 上海交通大学 | Chip gel electrophoresis and on-line UV-VIS imaging detection device thereof |
| CN111812091B (en) * | 2020-06-28 | 2023-09-05 | 上海交通大学 | Chip gel electrophoresis and online UV-VIS imaging detection device thereof |
| CN114324543A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Electrode and use thereof |
| CN114317238A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171027 |