CN206132618U - Induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis - Google Patents
Induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis Download PDFInfo
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- CN206132618U CN206132618U CN201620876163.0U CN201620876163U CN206132618U CN 206132618 U CN206132618 U CN 206132618U CN 201620876163 U CN201620876163 U CN 201620876163U CN 206132618 U CN206132618 U CN 206132618U
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Abstract
The utility model discloses an induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis, including polygenetic laser lamp -house group, polygenetic laser beam combining system, polygenetic laser focusing system, fluorescence collecting system, capillary holder and optical window, polychrome laser lamp -house component do not is " one " font interval distribution, and capillary holder and optical window correspond the setting respectively and be in polygenetic laser lamp -house group under, polygenetic laser beam combining system corresponds respectively and sets up under capillary holder and optical window, the polychrome laser focusing system configuration be in polygenetic laser beam combining systematic one side, fluorescence is collected system configuration and is in one side and the external computer of polygenetic laser focusing system. The utility model provides high sample test's efficiency and precision provides brand -new equipment platform for the development of multiple individualized kit moreover.
Description
Technical field
This utility model is related to technical field of fluorescence detection, more particularly to the multibeam laser light source based on capillary electrophoresis is induced
Fluorescence excitation device.
Background technology
With completing for the Human Genome Project, the mankind are unprecedented to having understood and grasped for itself hereditary information
It is progressive, while so that DNA detection technology has obtained fast development, the continuous improvement of DNA detection efficiency.DNA detection individual identification,
Highly important effect is played under the scenes such as blood relationship identification, criminal case detection, paternity test, disaster identification.
Capillary electrophoresis induced fluorescence method is detection method most widely used in DNA detection device in the market.It with
Elastic quartz capillary tube is split tunnel, with high-voltage dc as driving force so that the sample for preparing is in capillary tube
Realize that fragment is separated and produce unidirectional flowing, when laser is irradiated to the capillary detection window of setting position, sighting colorss
Fragment is discharged fluorescence, fluorescence quilt after the optical filter of specific wavelength by laser excitation to upper state while returning ground state
Light-sensitive detector is collected and is converted into the signal of telecommunication.So as to realize the collection of sample signal.Can be to labelling according to the signal of collection
Sample is analyzed.
More than current capillary electrophoresis fluorescence excitation device using single laser irradiation mode (as single 488nm laser,
532nm laser etc.), the method exists a little not enough：Single laser beam emitting device is used in inside spectra collection device and can only excite very
Several a variety of fluorescence, and wavelength less are closer to, and easily produce the phenomenon of fluorescence cross, and easily lose large stretch of segment information, lead
Cause is imperfect to signal collection, for analysis DNA data, is extremely disadvantageous.
Utility model content
The purpose of this utility model：The fluorescence excitation dress that a kind of multibeam laser light source based on capillary electrophoresis is induced is provided
Put, more rich fluorescence information can be obtained, fluorescence cross is solved the problems, such as.
To achieve these goals, the technical solution of the utility model is：
The fluorescence excitation device that multibeam laser light source based on capillary electrophoresis is induced, including Multi Colour Lasers light sources, polychrome
Laser closes beam system, Multi Colour Lasers focusing system, fluorescence gathering system, capillary tube holder and optical window；Multi Colour Lasers light
Source group is spaced apart in " one " font respectively, and capillary tube holder and optical window are correspondingly arranged at Multi Colour Lasers light sources respectively
Lower right, multiple Multi Colour Lasers close the left side that beam systems are correspondingly arranged at multiple capillary tube holders and optical window respectively；
Multi Colour Lasers focusing system is arranged on the right side that multiple Multi Colour Lasers close beam system, and fluorescence gathering system is arranged on Multi Colour Lasers and gathers
The side of burnt system external computer.
The Multi Colour Lasers light sources close beam system through Multi Colour Lasers and Multi Colour Lasers focusing system is radiated at capillary tube
On clamper and optical window, the fluorescence that sample sends is collected by fluorescence gathering system.
The axial direction of the Multi Colour Lasers light sources is axially vertical with described fluorescence gathering system.
This utility model improves the efficiency and precision of sample detection, and developing to multiple personal test kit
There is provided brand-new equipment platform.
Description of the drawings
Fig. 1 is the front view of the fluorescence excitation device that multibeam laser light source of this utility model based on capillary electrophoresis is induced.
Fig. 2 is the side view of the fluorescence excitation device that multibeam laser light source of this utility model based on capillary electrophoresis is induced.
Specific embodiment
Embodiment of the present utility model is further illustrated below in conjunction with accompanying drawing.
Refer to shown in accompanying drawing 1 and accompanying drawing 2, the fluorescence excitation device that the multibeam laser light source based on capillary electrophoresis is induced,
Beam system 2, Multi Colour Lasers focusing system 3, fluorescence gathering system 4, capillary tube are closed including Multi Colour Lasers light sources 1, Multi Colour Lasers
Clamper and optical window 5；Multi Colour Lasers light sources 1 are spaced apart in " one " font respectively, capillary tube holder and optical window
Mouth is correspondingly arranged at the lower right of Multi Colour Lasers light sources 1 respectively, and multiple Multi Colour Lasers conjunction beam systems 2 are correspondingly arranged at many respectively
The left side of individual capillary tube holder and optical window 5；Multi Colour Lasers focusing system 3 is arranged on multiple Multi Colour Lasers and closes beam system 2
Right side, fluorescence gathering system 4 is arranged on the side of capillary tube holder and optical window 5.
Described Multi Colour Lasers light sources 1 close beam system 2 through Multi Colour Lasers and Multi Colour Lasers focusing system 3 is radiated at hair
On tubule clamper and optical window 5, the fluorescence that sample sends is collected by fluorescence gathering system 4.
Described 1 Multi Colour Lasers of Multi Colour Lasers light sources close the axial direction of beam system 2 and the axle of described fluorescence gathering system 4
To vertical.
The step of being checked using this utility model is as follows：
1. the sample for preparing is input into into capillary tube.
2. cause sample that unidirectional electrophoresis is produced in capillary tube by high-voltage dc to separate with fragment.
3. capillary tube holder and optical window are irradiated to separately or together using Multi Colour Lasers light sources 1, excite sample
Product discharge fluorescence.
4. fluorescence is transferred to optical power detector through the collection of Multi Colour Lasers focusing system 3 and receives and be converted into the signal of telecommunication.
5. the signal of telecommunication of conversion is sent to analysis software by optical power detector, computer output image or number after analyzing and processing
According to.Due to being multigroup laser excitation, therefore need to combine corresponding many laser analysis methods, to be detected and analyzed sample.
Single Laser emission of capillary electrophoresis fluorescence spectrum harvester is changed to multibeam laser light source by this utility model, can be with
Individually launch the laser of different wave length, it is also possible to while launching the laser of multi-wavelength.Such that it is able to excite more kinds of wavelength
Fluorescence, to the compatible higher of sample reagent box.
In description of the present utility model, it is to be understood that term " lower section ", " side ", " axial direction ", " interior ", " outward "
Orientation or position relationship Deng instruction is, based on orientation shown in the drawings or position relationship, to be for only for ease of and describe this practicality newly
Type is described with simplifying, rather than is indicated or implied that the device or element of indication must be with specific orientation, with specific orientation
Construction and operation, therefore it is not intended that to restriction of the present utility model.
In description of the present utility model, " multiple " are meant that at least two, such as two, three etc., unless otherwise
It is clearly specific to limit.
In this utility model, unless otherwise clearly defined and limited, the art such as term " setting ", " distribution ", " external "
Language should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or it is integral；Can be that machinery connects
Connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, can be in two elements
The connection in portion or the interaction relationship of two elements, unless otherwise clearly restriction.For one of ordinary skill in the art
For, concrete meaning of the above-mentioned term in this utility model can be understood as the case may be.
In this utility model, unless otherwise clearly defined and limited, fisrt feature can be with second feature " lower section "
Be fisrt feature immediately below second feature or obliquely downward, or be merely representative of fisrt feature level height less than second feature.
In sum, this utility model improves the efficiency and precision of sample detection, and gives multiple personal test kit
Develop there is provided brand-new equipment platform.
Preferred embodiment of the present utility model is the foregoing is only, the scope of the claims of the present utility model is not thereby limited,
The equivalent structure transformation made by every utilization this utility model description, or it is directly or indirectly related with other are attached to
The technical field of product, is included in scope of patent protection of the present utility model in the same manner.
Claims (3)
1. the fluorescence excitation device that the multibeam laser light source based on capillary electrophoresis is induced, structure include：Multi Colour Lasers light sources
(1), Multi Colour Lasers close beam system (2), Multi Colour Lasers focusing system (3), fluorescence gathering system (4), capillary tube holder and light
Learn window (5)；It is characterized in that, Multi Colour Lasers light sources (1) are spaced apart in " one " font respectively, capillary tube holder and optics
Window (5) is correspondingly arranged at the lower right of Multi Colour Lasers light sources (1) respectively, and Multi Colour Lasers conjunction beam system (2) are corresponded to respectively and set
Put in the left side of capillary tube holder and optical window (5)；Multi Colour Lasers focusing system (3) is arranged on multiple Multi Colour Lasers and closes beam
The right side of system (2), optical power detector are arranged on the side of Multi Colour Lasers focusing system (3) external computer.
2. the fluorescence excitation device that the multibeam laser light source based on capillary electrophoresis according to claim 1 is induced, its feature
It is that described Multi Colour Lasers light sources (1) close beam system (2) and Multi Colour Lasers focusing system (3) irradiation through Multi Colour Lasers
In capillary tube holder and optical window (5), the fluorescence that sample sends is collected by fluorescence gathering system (4).
3. the fluorescence excitation device that the multibeam laser light source based on capillary electrophoresis according to claim 1 is induced, its feature
It is that the axial direction of described Multi Colour Lasers light sources (1) is axially vertical with described fluorescence gathering system (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620876163.0U CN206132618U (en) | 2016-08-15 | 2016-08-15 | Induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620876163.0U CN206132618U (en) | 2016-08-15 | 2016-08-15 | Induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206132618U true CN206132618U (en) | 2017-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620876163.0U Active CN206132618U (en) | 2016-08-15 | 2016-08-15 | Induced fluorescence trigger device of many laser lamp -house based on capillary electrophoresis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113237818A (en) * | 2021-05-28 | 2021-08-10 | 上海睿钰生物科技有限公司 | Cell analysis method and system |
-
2016
- 2016-08-15 CN CN201620876163.0U patent/CN206132618U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113237818A (en) * | 2021-05-28 | 2021-08-10 | 上海睿钰生物科技有限公司 | Cell analysis method and system |
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