CN205177772U - Light path device and mass spectrograph - Google Patents
Light path device and mass spectrograph Download PDFInfo
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- CN205177772U CN205177772U CN201520237014.5U CN201520237014U CN205177772U CN 205177772 U CN205177772 U CN 205177772U CN 201520237014 U CN201520237014 U CN 201520237014U CN 205177772 U CN205177772 U CN 205177772U
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Abstract
The utility model discloses a desorption of matrix auxiliary laser for the ionization sample arouse with the light path device of sample formation of image, the light path device includes external interface and 4 way mutually independent light paths, external interface connection is respectively based on the mass spectrum mass analyzer, 4 way light path of matrix auxiliary laser desorption ionization ion source: 2 way sample illumination light path, the sample excites the light path, and sample formation of image light path. According to the utility model discloses the light path device, its light path simple structure, and can combine sensitivity, exposure time, gain and the frame rate of the illumination brightness in the ion source cavity and the imaging device that selects and suitably adopt 1 way or 2 way throwing light on light paths and throw light on of corresponding to greatly improve the poor problem of illumination, make the sample obtain abundant illumination, be beneficial to quick, the clear realtime graphic of gaining the sample of imaging device.
Description
Technical field
The utility model belongs to analytical technique of mass spectrum field, particularly relates to a kind of light path device for substance assistant laser desorpted ionized sample excitation and imaging and mass spectrometer.
Background technology
1988, due to substance assistant laser desorpted ionized (MatrixAssistedLaserDesorptionIonization, be called for short MALDI) invention of technology, solve the ionization problem of large mass number biology, organic mass spectrometry is made to obtain breakthrough, start the biological macromolecular frontier of organic mass spectrometry research, greatly promote the research of Leaf proteins (a kind of cell, tissue or a complete set of protein corresponding to organism complete genome group).Large-scale separation and purification and identification and analysis are carried out to protein exactly in the field, forefront of current life science in cell or tissue level, mainly comprise: the protein that separation and purification is relevant to research purpose; The qualification of protein; The careful research of protein.Wherein, protein identifies it is key one step in proteomics research fast and accurately, has bridge beam action in proteomics.Because Matrix-assisted laser desorption ionization instrument (being called for short MALDI-TOF-MS) has the features such as quick, accurate, highly sensitive, flux is high, the mass range of detection is wide, be easy to correction, sample preparation is simple, structure easily simply is automated, become the first-selected instrument of proteomics Rapid identification.Due to the demand of rapid expansion, MALDI-TOF-MS market rapidly increased in the last few years, and instrument makes rapid progress, and the people of care and application also gets more and more.
Substance assistant laser desorpted ionized is a very complicated process, wherein relates to the processes such as optics, mechanics, thermodynamics and physical chemistry.Its general principle is dispersed in by analyte in matrix (Matrix) molecule and forms cocrystallization, when irradiating crystal with laser (N_2 laser of 337nm or the solid state laser of 355nm), sample molecule obtains laser energy desorption, Charger transfer occurs between matrix and sample and makes ionized sample molecule.Ion source uses pulsed laser; Produce single charge ion and the two single charge ion of part, the mass number one_to_one corresponding of the spectrum peak in mass spectrogram and each component of sample.
When using substance assistant laser desorpted ionized source ionized sample, first sample to be moved to the focus place of Laser Focusing, and need to observe the deposition conditions of sample, this just needs sample imaging optical path to meet fast, accurately, imaging demand clearly.And laser facula size, energy size and incident angle all can have influence on the abundance of sample ionization, and then affect mass spectrometric measuring range, sensitivity, resolution etc., so the design of sample excitation light path is also particularly important.
, there is light channel structure complexity, photographic brightness difference and debug the problems such as complicated difficulty in existing light path device.
Utility model content
The utility model is intended to solve one of technical problem in correlation technique at least to a certain extent.For this reason, an object of the present utility model is to propose a kind ofly have that light channel structure is simple, photographic brightness can effectively regulate to adapt to the substance assistant laser desorpted ionized sample excitation of different lighting requirement and the light path device of sample imaging.
According to the light path device of the utility model embodiment, for substance assistant laser desorpted ionized sample excitation and sample imaging, described light path device comprises the separate light path of external interface and 4 tunnels, described external interface connects based on substance assistant laser desorpted ionized ionogenic mass spectrum mass analyzer, and 4 tunnel light paths are respectively: 2 road illumination paths; Sample excitation light path; And sample imaging optical path.
According to the light path device of the utility model embodiment, by 2 road sample illumination light paths of separate setting, sample excitation light path and sample imaging optical path, make light channel structure simple; And can the sensitivity of brightness of illumination in the cavity of coupled ion source and selected image device, the time for exposure, gain and frame rate and suitably adopt 1 corresponding road or 2 road illumination paths to throw light on, thus greatly improve the problem of illumination difference, sample is thrown light on fully, is beneficial to the realtime graphic that image device obtains sample fast, clearly.
In addition, according to the light path device of the utility model above-described embodiment, following additional technical characteristic can also be had:
According to an embodiment of the present utility model, in described sample excitation light path, from ultraviolet wavelength pulse laser produce laser via the first fiber exit, and through collimating lens collimation, then line focus lens by Laser Focusing to sample place.It should be noted that, condenser lens described herein may also be the focus lens group be made up of multiple lens.When NES, condenser lens described in the utility model all contains condenser lens and this double implication of focus lens group.Adopt the laser of fiber optic conduction can better realize the adjustment of light path, and be convenient to mechanical erection fixed laser, realize the apparatus structure of concision and compact.
Preferably, described first optical fiber adopts core diameter to be 200um, NA is 0.23, joint is the optical fiber of SMA905.
Preferably, described collimating lens adopts planoconvex spotlight, and described condenser lens adopts planoconvex spotlight.
Preferably, described collimating lens and described condenser lens optical glass material used are far ultraviolet optical quartz glass.
According to an embodiment of the present utility model, in described sample imaging optical path, adopt imaging len that the reverberation of the sample be positioned on working plate is converged at image device place through glass filter, obtain the sharply defined image of sample, and record is stored in computer.
Preferably, wherein imaging len is biconvex lens.
Preferably, described glass filter adopts the golden yellow glass GG400 of ultraviolet cut-on type.
Preferably, described image device can adopt black-white CCD or CMOS as electrooptical device.
According to an embodiment of the present utility model, 2 road illumination paths separately adopt any one in the following two kinds design: light is conducted to sample place by (1) lighting source direct-coupling second optical fiber, and described second optical fiber is plastic fiber; (2) light is focused on sample place through collector lens by lighting source.
Preferably, described plastic fiber can adopt the low-loss plastic optical fiber of core diameter 2mm.
According to an embodiment of the present utility model, the 2 tunnel light paths being wherein in diagonal angle are positioned at same plane and the angle of itself and vertical direction is 30 °.
In addition, the purpose of this utility model is also to provide a kind of mass spectrometer with the light path device of above-mentioned any one embodiment.
Accompanying drawing explanation
Fig. 1 is the schematic appearance of the light path device according to the utility model enforcement;
Fig. 2 is the partial structurtes schematic diagram of the light path device according to the utility model embodiment;
Fig. 3 is the partial structurtes schematic diagram of the light path device according to another embodiment of the utility model;
Fig. 4 is the partial structurtes schematic diagram of the light path device according to another embodiment of the utility model;
Fig. 5 is the partial structurtes schematic diagram of the light path device according to another embodiment of the utility model;
Fig. 6 is the partial structurtes schematic diagram of the light path device according to other embodiments of the utility model;
Fig. 7 is the partial structurtes schematic diagram of the light path device according to the other embodiment of the utility model.
Embodiment
Be described below in detail embodiment of the present utility model, the example of described embodiment is shown in the drawings, and wherein same or similar label represents same or similar element or has element that is identical or similar functions from start to finish.Be exemplary below by the embodiment be described with reference to the drawings, be intended to for explaining the utility model, and can not be interpreted as restriction of the present utility model.
It should be noted that, in the utility model, term " first ", " second " only for describing object, and can not be interpreted as instruction or hint relative importance or imply the quantity indicating indicated technical characteristic.Thus, be limited with " first ", the feature of " second " can express or impliedly comprise at least one this feature.In description of the present utility model, the implication of " multiple " is at least two, such as two, three etc., unless otherwise expressly limited specifically.
Below, first composition graphs 1 describe according to the utility model embodiment, for substance assistant laser desorpted ionized sample excitation and sample imaging, light path device.
As shown in Figure 1, the separate light path 10,20,40,50 in external interface 30 and 4 tunnel is comprised according to the light path device of the utility model embodiment.
Wherein, external interface 30 connects based on substance assistant laser desorpted ionized ionogenic mass spectrum mass analyzer.
The separate light path in 4 tunnels is 2 road sample illumination light paths, sample excitation light path and sample imaging optical path respectively.The light path that Fig. 2 to Fig. 7 respectively illustrates 6 kinds of the utility model embodiments is arranged, as seen from the figure, in light path device of the present utility model, about the position relationship of 4 tunnel light paths, do not have special restriction, 2 road illumination paths wherein both can be adjacent, also can be that diagonal is arranged.
In following explanation, for convenience of explanation, only 10,20 to be illumination path, 40 to be described for sample imaging optical path for sample excitation light path, 50.
First, sample excitation light path 40 is described.
As shown in the figure, in sample excitation light path 40, the laser produced from ultraviolet wavelength pulse laser 401, via the first optical fiber 402 outgoing, and to collimate through collimating lens 403, then line focus lens 404 by Laser Focusing to sample 504 place.
Particularly, ultraviolet wavelength pulse laser 401 first collimates through collimating lens 403 after the first optical fiber 402 leaded light, laser-beam divergence angle is collimated to milli arc level, then through condenser lens 404, laser facula is focused to 100um.So not only can improve the energy of laser to ionize macromolecular sample, and can when minimum damage sample ionized sample molecule, obtain higher sensitivity.Here to expect that less laser facula can use the optical fiber that core diameter is thinner, or the focal distance ratio of larger collimating lens 403 and condenser lens (group) 404.Collimating lens 403 generally adopts planoconvex spotlight, and plane is towards optical fiber side, and condenser lens 404 also adopts planoconvex spotlight, and plane is towards sample direction, can reduce optical aberration to greatest extent like this.Consider ionogenic concrete structure, condenser lens 404 another planoconvex spotlight that sometimes needs to be coupled just can reach two aspect requirements of operating distance and little coupling focal length, therefore must be that focus lens group just can meet design requirement sometimes.Consider that the light of common optical glass to ultraviolet wavelength has stronger absorption, therefore all optical glass material of this sample excitation path can use far ultraviolet optical quartz glass (JGS1).
Next, sample imaging optical path is described with reference to the drawings.
As shown in the figure, in sample imaging optical path 50, the reverberation of the sample 504 being positioned on working plate 505 is converged at image device 501 place through glass filter 502 by imaging len 503, obtains the sharply defined image of sample, and record is stored in computer.
Wherein, imaging len 503 can be biconvex lens.
Glass filter 502 adopts the golden yellow glass GG400 of ultraviolet cut-on type.Glass filter 502 mainly plays three effects: one is the laser sponging a large amount of ultraviolet wavelengths, prevents it on the impact of sample imaging; Two are through the light of lighting source through sample reflection, are collected by the picture of sample smoothly by image device; Three is sealing ion source cavitys, because ion source is in ultralow vacuum (10
-7mbar) work under environment, therefore ion source must be sealed.The image information that image device 501 collects can record at any time and be saved in computer.
The image information that image device 501 collects can record at any time and be saved in computer.Image device 501 can adopt black-white CCD or CMOS as electrooptical device.
Next, with reference to accompanying drawing, illumination path 10,20 is described.It should be noted that, illumination path 10,20 independently can take the following two kinds mode, and for the purpose of simplifying the description, this sentences illumination path 20 for example and is described.
Single with regard to illumination path 20, its execution mode has two kinds: (1) as shown in Figure 2, light is conducted to sample 504 place by lighting source 201 direct-coupling second optical fiber and plastic fiber 202, illuminates sample 504; (2) as shown in Figure 3, lighting source 201 is through collector lens 203, and the light sent by light source converges at sample 504 place.The sample area that front a kind of light path can illuminate is larger, but its brightness is more weak, and with the difference of image device 501, the brightness of imaging has a great difference, sometimes can't see the picture of sample even completely.Although the brightness of rear a kind of light path sample promotes very large, can the region illuminating sample is often too little.What kind of illumination path of concrete selection is according to the specific requirement of Instrument Design and the performance of image device.
In addition, in order to improve these two kinds of illumination paths shortcoming separately, can design as Fig. 4-7 etc.
In Fig. 4,10 and 20 passages are all adopted the first light path design, can the brightness of irradiating sample be expanded to original twice like this.
Fig. 5 illumination path 20 and imaging optical path 50 meet the reflection law of light, so also can greatly improve the brightness entering image device.
Fig. 6, when illumination path 20 meets light reflection law with imaging optical path 50, adopts the design of the second illumination path to can further improve the brightness entering image device.
Fig. 7 is Zai Jia mono-tunnel illumination (namely simultaneously adopting illumination path 10 and 20) on the basis of Fig. 5, thus can further improve the brightness entering image device.
Ion source design of the present utility model has four tunnel path channels, and wherein two paths is used for sample excitation and sample imaging, and another two paths is illumination path.The utility model adopts sample excitation, sample imaging and sample illumination light path design independent of each other, the illumination of a-road-through road can be applied as required, or two-way is all used for illuminated sample, and the light path of sample illumination has two kinds of designs, respectively there is its feature, specific embodiment, can determine according to actual conditions.
The utility model is particularly suitable for being applied to substance assistant laser desorpted ionized mass spectrometric ion source light path design, that is, provide a kind of mass spectrometer with above-mentioned light path device, this mass spectrometer is substance assistant laser desorpted ionized mass spectrometer.
In the description of this specification, specific features, structure, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present utility model or example.And the specific features of description, structure, material or feature can combine in one or more embodiment in office or example in an appropriate manner.In addition, when not conflicting, the feature of the different embodiment described in this specification or example and different embodiment or example can carry out combining and combining by those skilled in the art.
Although illustrate and described embodiment of the present utility model above, be understandable that, above-described embodiment is exemplary, can not be interpreted as restriction of the present utility model, those of ordinary skill in the art can change above-described embodiment, revises, replace and modification in scope of the present utility model.
Claims (13)
1. a light path device, for substance assistant laser desorpted ionized sample excitation and sample imaging, it is characterized in that, described light path device comprises the separate light path of external interface and 4 tunnels, described external interface connects based on substance assistant laser desorpted ionized ionogenic mass spectrum mass analyzer, and 4 tunnel light paths are respectively:
2 road illumination paths;
Sample excitation light path; And
Sample imaging optical path.
2. light path device according to claim 1, it is characterized in that, in described sample excitation light path, from ultraviolet wavelength pulse laser produce laser via the first fiber exit, and through collimating lens collimation, then line focus lens by Laser Focusing to sample place.
3. light path device according to claim 2, is characterized in that, it is 0.23 that described first optical fiber adopts core diameter to be 200um, NA, joint is the optical fiber of SMA905.
4. light path device according to claim 2, is characterized in that, described collimating lens adopts planoconvex spotlight, and described condenser lens adopts planoconvex spotlight.
5. light path device according to claim 2, is characterized in that, described collimating lens and described condenser lens optical glass material used are far ultraviolet optical quartz glass.
6. light path device according to claim 1, it is characterized in that, in described sample imaging optical path, adopt imaging len that the reverberation of the sample be positioned on working plate is converged at image device place through glass filter, obtain the sharply defined image of sample, and record is stored in computer.
7. light path device according to claim 6, is characterized in that, wherein imaging len is biconvex lens.
8. light path device according to claim 6, is characterized in that, described glass filter adopts the golden yellow glass GG400 of ultraviolet cut-on type.
9., according to light path device according to claim 6, described image device can adopt black-white CCD or CMOS as electrooptical device.
10. light path device according to claim 1, is characterized in that, the one during described 2 road illumination paths separately adopt the following two kinds to design:
(1) light is conducted to sample place by lighting source direct-coupling second optical fiber, and described second optical fiber is plastic fiber;
(2) light is focused on sample place through collector lens by lighting source.
11. light path devices according to claim 10, is characterized in that, described plastic fiber can adopt the low-loss plastic optical fiber of core diameter 2mm.
12. light path devices according to any one of claim 1-11, is characterized in that, the 2 tunnel light paths being wherein in diagonal angle are positioned at same plane and the angle of itself and vertical direction is 30 °.
13. 1 kinds of mass spectrometers, is characterized in that, described mass spectrometer comprises the light path device according to any one of claim 1-12.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520237014.5U CN205177772U (en) | 2015-04-17 | 2015-04-17 | Light path device and mass spectrograph |
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| CN201520237014.5U CN205177772U (en) | 2015-04-17 | 2015-04-17 | Light path device and mass spectrograph |
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| CN205177772U true CN205177772U (en) | 2016-04-20 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104795306A (en) * | 2015-04-17 | 2015-07-22 | 江苏天瑞仪器股份有限公司 | Sample stimulating and sample imaging light path device for matrix assisted laser desorption ionization |
| CN107658205A (en) * | 2017-09-29 | 2018-02-02 | 珠海美华医疗科技有限公司 | A kind of MALDI light paths and high voltage electric field bringing device and mass spectrograph |
| CN109712862A (en) * | 2019-01-28 | 2019-05-03 | 安图实验仪器(郑州)有限公司 | Light path system suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument |
-
2015
- 2015-04-17 CN CN201520237014.5U patent/CN205177772U/en not_active Ceased
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104795306A (en) * | 2015-04-17 | 2015-07-22 | 江苏天瑞仪器股份有限公司 | Sample stimulating and sample imaging light path device for matrix assisted laser desorption ionization |
| CN107658205A (en) * | 2017-09-29 | 2018-02-02 | 珠海美华医疗科技有限公司 | A kind of MALDI light paths and high voltage electric field bringing device and mass spectrograph |
| CN107658205B (en) * | 2017-09-29 | 2024-05-24 | 珠海美华医疗科技有限公司 | Light path and high-voltage electric field applying device for MALDI and mass spectrometer |
| CN109712862A (en) * | 2019-01-28 | 2019-05-03 | 安图实验仪器(郑州)有限公司 | Light path system suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument |
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Decision date of declaring invalidation: 20181205 Decision number of declaring invalidation: 38143 Granted publication date: 20160420 |