CN205844186U - A kind of portable capillary pipe electrophoresis laser induced fluorescence detector - Google Patents
A kind of portable capillary pipe electrophoresis laser induced fluorescence detector Download PDFInfo
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- CN205844186U CN205844186U CN201620577590.9U CN201620577590U CN205844186U CN 205844186 U CN205844186 U CN 205844186U CN 201620577590 U CN201620577590 U CN 201620577590U CN 205844186 U CN205844186 U CN 205844186U
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- capillary tube
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- induced fluorescence
- capillary
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Abstract
The utility model discloses a kind of portable capillary pipe electrophoresis laser induced fluorescence detector, including a laser-induced fluorescence (LIF) Systems for optical inspection equipped with semiconductor laser and signal processing unit, side in laser-induced fluorescence (LIF) Systems for optical inspection optical window, fixedly mounting a replaceable capillary tube fixing device, this replaceable capillary tube fixing device forms by capillary tube holder with for finely tuning the accurate translation stage of this capillary tube holder；Capillary tube holder is fixed on the removable slide block of accurate translation stage, and makes the capillary tube fluoroscopic examination window on capillary tube holder be positioned at the optical window detection mouth position of laser-induced fluorescence (LIF) Systems for optical inspection.This utility model is by replaceable capillary tube fixing device, when being used for quickly detecting work, more changing jobs of capillary tube can be rapidly completed upon completion of the assays, and utilize accurate translation stage that capillary detection window is accurately adjusted to optimum detection position, thus significantly improve the work efficiency of portable capillary pipe electrophoresis.
Description
Technical field
This utility model belongs to technical field of analysis and detection, relates to a kind of capillary electrophoresis structure design, specifically relates to
And one portable capillary pipe electrophoresis laser induced fluorescence detector.
Background technology
Capillary electrophoresis apparatus is the novel analytical tool of one grown up the eighties in last century, in analytical tool development history
On there is epoch-making meaning.It is driving force that capillary electrophoresis (CE) is based on high voltage electric field, with capillary tube as split tunnel
Novel liquid-phase separate analytical technique, it is to realize based on mobility between component each in sample and the difference in distribution behavior separating
A liquid-like phase isolation technics.Owing to capillary electrophoresis has the highest post effect, typically can reach hundreds of thousands theoretical cam curve；Spirit
Sensitivity is high, uses laser-Induced Fluorescence Detection method and Amperometric Detection Coupled method all can detect to 10-19mol·L-1, even up to 10- 21mol·L-1；Separating rate is exceedingly fast, and the most only needs within tens seconds, just can complete to separate to dozens of minutes；Additionally, capillary electrophoresis
Also having sample size few, solvent consumption is few, and instrument is simple, low cost and other advantages；Analyze object relate to inorganic ions, organic little point
Son, biomacromolecule etc.；And capillary column expense compared with HPLC column or GC post is much lower.Capillary electrophoresis apparatus by people from
Freeing in numerous and diverse tediously long conventional electrophoretic method, (analysis time reduced from several hours to drastically increase work efficiency
To 5～20min), greatly reduce amount of samples (being reduced to a nanoliter level from micro updating), and quantitative level is brought up to one newly
Height.In many fields such as biochemistry, molecular biology, Food Chemistry, pharmaceutical chemistry, Environmental Chemistry, medical science and the administration of justice
Gradually it is used widely.CE detection is easily achieved miniaturization, normal for containing the toxic residual sample of stronger agriculture of this point
Rule detection is extremely important.Thus while capillary electrophoresis separates starting late of analysis for food pesticide residues, but show
Going out great potentiality, development in recent years is the most extremely rapid.
But, owing to current capillary electrophoresis apparatus mostly is universal instrument, instrument cost is higher, need to place laboratory and make
It is to take back laboratory further to sample analysis after field investigation and sampling by, common working method, it is impossible at sampling onsite application,
Therefore the popularization and application of this technology are limited.The feature that this mode of operation makes high performance capillary electrophoresis quickly analyze simultaneously is difficult to
Give full play to, limit the popularization and application of the method.Capillary electrophoresis apparatus is generally by capillary tube, high voltage power supply and capillary electrophoresis
Detector forms, and wherein capillary tube electrophoresis detector is its core.In recent years, the grinding of portable capillary pipe electrophoresis detector
System gradually causes the concern of people.Compared with normal capillary electrophoresis tube detector, portable capillary pipe electrophoresis detector is the most obvious
Feature be that the integrated level of instrument is higher, and use small-sized components and parts." a kind of portable capillary pipe as disclosed in Lin Jinming etc.
Electrophresis apparatus " (CN 101334378 B) patent of invention, it uses pen type mercury lamp to be that light source instead of in normal capillary electrophoresis tube instrument
Conventional deuterium lamp light source, substantially reduces instrument volume, improves the portability of instrument.In addition, domestic associated portable
Capillary tube electrophoresis detector is little, and detection device mostly uses ultraviolet detection, causes detection sensitivity relatively low.In detection mode
On, still using the standard mode of laboratory capillary electrophoresis apparatus, detection efficiency is relatively low.
Existing portable capillary pipe electrophoresis apparatus mostly uses ultraviolet detection, the principle of this detection mode to be according to sample pair
Absorbance be analyzed, due to the restriction of the sample absorption coefficient of light, the sensitivity determining this detection mode is relatively low.And
The detection mode utilizing laser-induced fluorescence (LIF) can be greatly improved the sensitivity of detection.Meanwhile, along with semiconductor laser in recent years
The development of technology, the semiconductor laser commercialization in succession of various wavelength miniaturization so that use laser-Induced Fluorescence Detection skill
The portable capillary pipe electrophoresis apparatus of art is possibly realized.Meanwhile, existing capillary electrophoresis testing process, including sample introduction, detects, cleans
Several steps.The usually more than working time of half is all used for cleaning capillary tube.This substantially prolongs capillary electrophoresis apparatus detection
Time, seriously limit the raising of capillary electrophoresis apparatus detection efficiency.
Optical maser wavelength used by current laser induced fluorescence detector is usually the long wave blue light of 473nm or 488nm
Laser instrument.These laser instrument volumes are big, and the volume causing detector overall is the biggest, is not suitable for the use of portable instrument.
Summary of the invention
For these reasons, the purpose of this utility model is to provide a kind of simple in construction, and easy to install is portable
Capillary electrophoresis laser induced fluorescence detector, it uses new microization blue light or royal purple optical semiconductor laser as light
Source, combine the setting of replaceable capillary tube fixing device, solve the problems referred to above that existing capillary electrophoresis technique exists
This utility model be employed technical scheme comprise that, a kind of portable capillary pipe electrophoresis laser induced fluorescence detector,
Including a laser-induced fluorescence (LIF) Systems for optical inspection A equipped with semiconductor laser and signal processing unit, in described laser
The side of induced fluorescence Systems for optical inspection A optical window, fixedly mounts replaceable capillary tube fixing device B, and described is replaceable
Capillary tube fixing device B, forms by capillary tube holder with for finely tuning the accurate translation stage of this capillary tube holder；Capillary tube holder
It is fixed on the removable slide block of accurate translation stage, and makes the capillary tube fluoroscopic examination window on capillary tube holder be positioned at sharp
The optical window detection mouth position of light induced fluorescence Systems for optical inspection A；
Described laser-induced fluorescence (LIF) Systems for optical inspection A, semiconductor laser therein uses wavelength to be 440～450nm
Blue semiconductor laser.
Portable capillary pipe electrophoresis laser induced fluorescence detector described in the utility model, is further characterized in that,
Described laser-induced fluorescence (LIF) Systems for optical inspection A, semiconductor laser therein use wavelength or be 360～
The royal purple optical semiconductor laser of 410nm.
This utility model portable capillary pipe electrophoresis laser induced fluorescence detector, by examining in laser-induced fluorescence (LIF) optics
The hard-wired replaceable capillary tube fixing device in side of examining system optical window is when needs are used for quickly detecting work, permissible
After one-time detection completes, use the capillary tube holder that pre-wash is good, polylith is standby, be rapidly completed the replacing work of capillary tube
Make, and utilize accurate translation stage that capillary detection window thereon is accurately adjusted to laser-induced fluorescence (LIF) Systems for optical inspection optical window
The optimum detection position of window；The detection and the capillary duct cleaning that make capillary electrophoresis can synchronize to carry out, thus
The work efficiency of portable capillary pipe electrophoresis can be significantly improved.
This utility model portable capillary pipe electrophoresis laser induced fluorescence detector, uses miniaturization blue light or royal purple light to make
For semiconductor laser as light source, optimize light path design, thus reduce instrument volume so that instrument entirety is easy to carry
And application, substantially increase portability.Simultaneously because the use of short wavelength laser, most of classical long wavelength not only can be used to swash
The derivative reagent sent out, is also applied for royal purple light activated short wavelength derivative reagent.
Accompanying drawing explanation
Fig. 1 is this utility model portable capillary pipe electrophoresis laser induced fluorescence detector structural representation；
Fig. 2 is this utility model replaceable capillary tube fixture structure schematic diagram.
In figure, 1. semiconductor laser, 2. anti-blue saturating red green light splitting eyeglass, the most left lens, 4. optical window, 5. accurate translation
Platform, 5 '. removable slide block, 6. capillary tube holder, 7. capillary detection window, the rightest lens, 9. diaphragm, 10. photomultiplier tube,
11. capillary tubies.
Detailed description of the invention
With detailed description of the invention, this utility model is described in detail below in conjunction with the accompanying drawings.
A kind of portable capillary pipe electrophoresis laser induced fluorescence detector, as depicted in figs. 1 and 2, it include one equipped with
The laser-induced fluorescence (LIF) Systems for optical inspection A of semiconductor laser 1 and signal processing unit, in described laser-induced fluorescence (LIF) optics
The side of detecting system A optical window 4, fixedly mounts replaceable capillary tube fixing device B, the fixing dress of described replaceable capillary tube
Put B, form by capillary tube holder 6 with for finely tuning the accurate translation stage 5 of this capillary tube holder 6；Capillary tube holder 6 is installed fixing
On the removable slide block 5 ' of accurate translation stage 5, and make the capillary detection window 7 of capillary tube 11 on capillary tube holder 6
Optical window 4 in laser-induced fluorescence (LIF) Systems for optical inspection A detects mouth position.
This utility model laser-induced fluorescence (LIF) Systems for optical inspection A, semiconductor laser 1 therein uses wavelength to be 440
～the blue semiconductor laser of 450nm；Or the royal purple optical semiconductor laser using wavelength to be 360～410nm.
This utility model portable capillary pipe electrophoresis laser induced fluorescence detector, when detecting, as it is shown in figure 1,
Blueness that semiconductor laser 1 sends or bluish violet color laser, the window on light shielding mask be radiated at install in 45° angle anti-blue thoroughly
On red green light splitting eyeglass 2, assembling through left lens 3 after being reflected, the light transmission optical window 4 of convergence focuses on capillary tube electricity after detecting mouth
At the capillary detection window 7 of swimming detection；It is same that what the sample at capillary detection window 7 sent after being optically excited dissipates fluorescence
It is transformed into directional light after being collected by left lens 3 after optical window 4, then focuses on the aperture on diaphragm 9 after right lens 8 are assembled
On.Owing to the most veiling glares in device are all without being irradiated at this aperture, the interference of veiling glare therefore can be made by greatly
Weaken greatly；Fluorescence on diaphragm 9 unthreaded hole is irradiated to be fixed on photomultiplier tube 10 thereafter, examines through photomultiplier tube 10
After measured light intensity, the gained signal of telecommunication is processed by signal processing system further.
After one-time detection completes, used capillary tube holder 6 can be unloaded, then the capillary tube that a piece standby is propped up
Frame 6 is changed, and is rapidly completed more changing jobs of capillary tube 11, then utilizes the capillary tube inspection that accurate translation stage 5 will be fixed thereon
Survey window 7 and be accurately adjusted to the optimum detection position of laser-induced fluorescence (LIF) Systems for optical inspection A optical window 4 window；Carry out next time
Laser-Induced Fluorescence Detection.
This utility model portable capillary pipe electrophoresis detector, in order to improve analysis speed, uses changeable type capillary tube solid
Determining device B, as shown in Figure 2, capillary tube 11 can be fixed in replaceable capillary tube mounting bracket 6 in a suitable manner, for protecting
Card capillary detection window 7 is accurately located exactly in the focus of exciting light, every time before detection, first will be equipped with capillary tube 11 with screw
Replaceable capillary tube holder 6 be tentatively fixed on the removable slide block 5 ' of accurate translation stage 5, then by rotating precision translation
The adjustment screw of platform 5 adjusts capillary detection window 7, observes corresponding fluorescence intensity signals by signal processing system simultaneously,
When signal intensity is transferred to maximum, i.e. explanation capillary detection window 7 has been adjusted to optimum position, thus ensures to use replaceable
Formula capillary tube obtains good detection sensitivity.
The accurate translation stage 5 of this utility model replaceable capillary tube fixing device B can quick glimmering by capillary tube 11
Light detection window is accurately adjusted to focal position of laser, to obtain high-sensitive fluoroscopic examination, considerably improves capillary tube electricity
The work efficiency of swimming.
This utility model portable capillary pipe electrophoresis laser induced fluorescence detector, wherein semiconductor laser 1 uses ripple
A length of 440～the blue semiconductor laser of 450nm, or the royal purple photosemiconductor laser using wavelength to be 360～410nm
Device, optimizes light path design, makes semiconductor laser 1 be miniaturized, thus reduces volume so that detecting instrument entirety is easy to take
Band and application, substantially increase portability.Due to the use of short wavelength laser, most of classical long-wavelength excitation not only can be used
Derivative reagent, it is also possible to for royal purple light activated short wavelength derivative reagent.
Above-mentioned embodiment is an example of the present utility model, is not intended to limit enforcement and the right model of invention
Enclose, all equivalence changes made according to the content described in this utility model patent protection scope and modification, all should be included in
In this utility model claim.
Claims (2)
1. a portable capillary pipe electrophoresis laser induced fluorescence detector, including swashing equipped with semiconductor laser (1)
Light induced fluorescence Systems for optical inspection A and signal processing unit, it is characterised in that in described laser-induced fluorescence (LIF) optical detection system
The side of system A optical window (4), fixed installation one replaceable capillary tube fixing device B, described replaceable capillary tube fixing device B,
By capillary tube holder (6) be used for finely tuning the accurate translation stage (5) of this capillary tube holder (6) and form；Capillary tube holder (6) is installed
It is fixed on the removable slide block (5 ') of accurate translation stage (5), and makes capillary tube (11) fluoroscopic examination on capillary tube holder (6)
Window (7) is positioned at optical window (4) the detection mouth position of laser-induced fluorescence (LIF) Systems for optical inspection A；
Described laser-induced fluorescence (LIF) Systems for optical inspection A, semiconductor laser therein (1) use optical maser wavelength be 440～
The blue semiconductor laser of 450nm.
Portable capillary pipe electrophoresis laser induced fluorescence detector the most according to claim 1, it is characterised in that described sharp
Light induced fluorescence Systems for optical inspection A, semiconductor laser therein (1) use wavelength or be 360～410nm royal purple light half
Conductor laser.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620577590.9U CN205844186U (en) | 2016-06-14 | 2016-06-14 | A kind of portable capillary pipe electrophoresis laser induced fluorescence detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620577590.9U CN205844186U (en) | 2016-06-14 | 2016-06-14 | A kind of portable capillary pipe electrophoresis laser induced fluorescence detector |
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| CN205844186U true CN205844186U (en) | 2016-12-28 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108152262A (en) * | 2018-01-11 | 2018-06-12 | 南京溯远基因科技有限公司 | A kind of Capillary Electrophoresis method for nucleic acid analysis and system |
| CN110044875A (en) * | 2019-04-26 | 2019-07-23 | 南京信息工程大学 | A kind of aerosol detection sighting device for laser induced breakdown spectroscopy |
| CN110520721A (en) * | 2017-02-24 | 2019-11-29 | 生命技术公司 | Optical system and method for sample separation |
-
2016
- 2016-06-14 CN CN201620577590.9U patent/CN205844186U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110520721A (en) * | 2017-02-24 | 2019-11-29 | 生命技术公司 | Optical system and method for sample separation |
| CN108152262A (en) * | 2018-01-11 | 2018-06-12 | 南京溯远基因科技有限公司 | A kind of Capillary Electrophoresis method for nucleic acid analysis and system |
| CN110044875A (en) * | 2019-04-26 | 2019-07-23 | 南京信息工程大学 | A kind of aerosol detection sighting device for laser induced breakdown spectroscopy |
| CN110044875B (en) * | 2019-04-26 | 2024-04-19 | 南京信息工程大学 | Aerosol detection aiming device for laser-induced breakdown spectroscopy technology |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161228 Termination date: 20190614 |