CN203300593U - Laser introducing and sample imaging apparatus for MALDI source - Google Patents
Laser introducing and sample imaging apparatus for MALDI source Download PDFInfo
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- CN203300593U CN203300593U CN2013202176729U CN201320217672U CN203300593U CN 203300593 U CN203300593 U CN 203300593U CN 2013202176729 U CN2013202176729 U CN 2013202176729U CN 201320217672 U CN201320217672 U CN 201320217672U CN 203300593 U CN203300593 U CN 203300593U
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Abstract
The utility model provides a laser introducing and sample imaging apparatus for a matrix-assisted laser desorption ionization source, and the apparatus comprises a laser introducing device and a sample imaging device. The laser introducing device leads laser to a sample area to ionize sample, and the sample imaging device enables the sample area in an ionization chamber to be visible. Three independent optical paths for laser, illumination and imaging are employed. A specific fiber is used to conduct laser, the optical system is simplified, and the profile design of the matrix-assisted laser desorption ionization source is convenient. The laser introducing and sample imaging apparatus is suitable for application to an MALDI-TOF mass spectrometer.
Description
Technical field
The utility model belongs to the analytical technique of mass spectrum field, is specifically related to the device of a kind of introducing of laser for substance assistant laser desorpted ionized source and sample imaging, is applicable to the mass spectrometric ion source of MALDI-TOF.
Background technology
Mass spectrometer is that a class can make the material particle ionization become ion and by suitable electric field, magnetic field, whether they be realized to the mass-to-charge ratio separation by locus, time order and function or orbitally stable, and after detected intensity, carries out the instrument of species analysis.Ion source is one of mass spectrometric core.Ion source, be to make neutral atom or molecular ionization, and therefrom draw the device of ion beam current.Emerging in recent years the soft ionization technology that is successfully used to the large biological molecule mass spectral analysis mainly contains following several: 1) electrospray ionization mass spectrometry; 2) substance assistant laser desorpted ionized mass spectrum; 3) fast atom bombardment mass spectroscopy; 4) ionspray MALDI-MS; 5) atmospheric pressure ionization mass spectrometry.In these soft ionization technology, with three kinds of fronts Recent study, obtain at most, apply also the most extensively.
Matrix assisted laser desorption ionization mass-spectrometric technique (Mat rix Assisted Laser Desorption/Ionization, MALDI) basic principle is be dispersed in analyte in substrate molecule and form crystal, when with the Ear Mucosa Treated by He Ne Laser Irradiation crystal, the energy that absorbs through radiation due to substrate molecule, cause energy to be accumulated and heat production rapidly, thereby make the host crystal distillation, cause matrix and analyte expand and enter gas phase.The mass spectrogram that MALDI produces mostly is single charge ion, thereby the quality of the ion in mass spectrogram and peptide and protein has one-to-one relationship.The MALDI-TOF mass spectrum is well suited for the research to large biological molecules such as protein, polypeptide, nucleic acid and polysaccharide.
When using substance assistant laser desorpted ionized source ionized sample, need to observe the zone of sample panel and the deposition conditions of sample.At document " ION FORMATION IN MALDI MASS SPECTROMETRY " thereby in mention the size of laser facula, the size of laser energy, the abundance that laser incident angle all can have influence on sample ionization and affect mass spectrometric measuring range, sensitivity, resolution.
Technology contents
The invention provides the device of a kind of introducing of laser for substance assistant laser desorpted ionized source and sample imaging, this device comprises laser introducing device and sample imaging device.Thereby described laser introducing device is guided laser into the sample area ionized sample, and the sample imaging device is realized the visible of the interior sample area of ionization chamber.
Described laser introducing device comprises optical attenuator, optical fiber, optical adjusting bracket, laser beam expanding instrument, laser mirror, the condenser lens of laser, gradual change.The output of laser is provided with the optical attenuator of gradual change, laser after decay passes through fiber optic conduction, at fiber-optic output, be provided with laser beam expander, the laser beam expander end is provided with optical adjusting bracket, output laser through speculum with plane, sample panel place, to become the direction of miter angle to enter ionization chamber, in ionization chamber, be provided with condenser lens, make laser with certain hot spot, arrive sample area through these lens.Laser attenuation wherein and laser beam expanding element are arranged on the outside of ionization chamber, and laser focusing lens is arranged on the inside of ionization chamber, on the concrete battery lead plate that is arranged on mass analyzer.
The ionization energy that the ionized sample molecule of different molecular weight needs is different, and the sample molecule of molecular weight needs less ionization energy, and the optical attenuator of gradual change can change the size of laser energy continuously, thereby obtains wider mass range.To be in order to obtain meeting the hot spot of crystallized sample ionization demand from laser beam expanding and then the focusing of optical fiber, deriving.The crystallization result of different matrix and sample is different, and therefore different matrix crystallizations needs specific laser facula.The laser facula that only is complementary could, in the situation that the damage sample of minimum ionizes, and obtain higher sensitivity.Adopt fiber optic conduction can better realize the light beam bending, locating laser outbound course more accurately, laser conducts in optical fiber loss will be lower than the loss that is come by optical element light conducting band.
Described sample imaging device comprises illumination LED, speculum, speculum, CCD, computer.At first light beam from the illuminating LED lamp emission enters ionization chamber, then by speculum, vertically guides light into sample area.The reverberation of sample area is drawn ionization chamber through the another one speculum by light and is arrived imaging CCD acquisition interface, and the sample image that this CCD gathers by the data importing computer, is seen the image of sample area by the USB line by computer.
Speculum is arranged at the upper end of mass analyzer, the left side of ion beam, and speculum is positioned at the upper end of mass analyzer, and the right side of ion beam does not affect the movement locus of ion.
This device of the present invention adopts independently light path design of three of laser, illumination, imaging.
The advantage of apparatus of the present invention is that the fiber optic conduction laser energy better realizes light beam bending, locating laser outbound course more accurately, and laser conducts in optical fiber loss will be lower than the loss that is come by optical element light conducting band; Laser beam expanding focuses on the method that obtains hot spot again can, in the situation that the minimum damage sample ionizes, and obtain higher sensitivity; The optical attenuator of gradual change can change the size of laser energy continuously, thereby obtains wider mass range; The design of part optical element can be simplified to the design of instrument ionization source in ionization chamber inside, make the instrument volume less.
The accompanying drawing explanation
Fig. 1 is that a kind of laser of substance assistant laser desorpted ionized source is introduced and the schematic diagram of the device of sample imaging.
Wherein 1 is laser, and 2 are the optical attenuator of gradual change, and 3 is optical fiber, 4 is laser beam expander, and 5 is optical adjusting bracket, and 6 is laser mirror, 7 is condenser lens, and 8 is sample panel, and 9 is mass analyzer, 10 is ion beam, and 11 is ionization chamber, and 12 is illumination LED, 13 is speculum, 14 is speculum, and 15 is IMAQ CCD, and 16 is computer.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
As shown in Figure 1, the invention provides the device of a kind of introducing of laser for substance assistant laser desorpted ionized source and sample imaging, this device comprises laser introducing device and sample imaging device.The laser introducing device is guided laser into sample, thereby ionized sample is ionization source.The sample imaging device is realized the visible of the interior sample area of ionization chamber.
Described laser introducing device comprises optical attenuator 2, optical fiber 3, laser beam expander 4, optical adjusting bracket 5, laser mirror 6, sample panel 8, ionization chamber 11, the condenser lens 7 of ionization source laser 1, gradual change.The output of laser 1 is provided with the optical attenuator 2 of gradual change, laser after decay is by optical fiber 3 conduction, at optical fiber 3 outputs, be provided with laser beam expander 4, laser beam expander 4 ends are provided with optical adjusting bracket 5, output laser through speculum 6 with sample panel 8 planes, place, to become the direction of miter angle to enter ionization chamber 11, in ionization chamber 11, be provided with condenser lens 7, make laser with certain hot spot, arrive sample area through these lens.
Wherein laser 1 is the nitrogen laser, and the Output of laser wavelength is 337nm.The optical attenuator 2 of gradual change adopts circular variable metal neutral-density filter, has minimum loss.Sample panel 8 levels be positioned at ionization chamber 11.The condenser lens 7 that is arranged in ionization chamber 11 is arranged on the battery lead plate of mass analyzer 9.The direction of laser in space of can regulating the optical adjusting bracket 5 of laser beam expander 4 end settings realizes that thereby laser alignment makes laser accurately arrive sample area.Condenser lens 7 can be selected biconvex or planoconvex spotlight.
Ionization chamber laser can be entered in another embodiment to plane, place, hole is designed to and sample panel 45 degree, can directly the laser beam expander Output of laser be introduced in ionization chamber without speculum like this, recycling optical adjusting bracket fine setting laser incident angle, reduce laser loss.
Described sample imaging device comprises illumination LED 12, speculum 13, speculum 14, ccd image collector 15, computer 16.At first light beam from illuminating LED lamp 12 emissions enters ionization chamber 11, then by speculum 13, vertically guides light into sample area.The reverberation of sample area is drawn ionization chamber 11 by light and arrives ccd image collector 15 acquisition interfaces through speculum 14, and the sample image that ccd image collector 15 gathers by data importing computer 16, is seen the image of sample area by the USB line by computer 16.
Device of the present invention adopts independently light path design of three of laser, illumination, imaging.Unique fiber optic conduction laser, simplify optical system, is convenient to the configuration design in substance assistant laser desorpted ionized source.The present invention is suitable for being applied to the MALDI-TOF mass spectrometer and makees ion source.
Claims (9)
1. the laser for the MALDI source is introduced and the device of sample imaging, this device comprises laser introducing device and sample imaging device, it is characterized in that the output of laser (1) is provided with the optical attenuator (2) of gradual change, laser after decay is by optical fiber (3) conduction, at optical fiber (3) output, be provided with laser beam expander (4), laser beam expander (4) end is provided with optical adjusting bracket (5), output laser through speculum (6) with sample panel (8) plane, place, to become the direction of miter angle to enter ionization chamber (11), in ionization chamber (11), be provided with condenser lens (7), make laser with certain hot spot, arrive sample area through these lens.
2. device according to claim 1, wherein sample panel (8) level is positioned at ionization chamber (11).
3. device according to claim 1, wherein condenser lens (7) is arranged in ionization chamber (11) and is positioned on the battery lead plate of mass analyzer (9).
4. device according to claim 3, wherein condenser lens (7) can be biconvex or planoconvex spotlight.
5. device according to claim 1, wherein ionization chamber (11) laser can be entered to plane, place, hole and be designed to become with sample panel (8) 45 degree, can directly laser beam expander (4) Output of laser be introduced in ionization chamber (11) without speculum like this to recycling optical adjusting bracket (5) fine setting laser incident angle.
6. device according to claim 1, wherein at first the light beam from illuminating LED lamp (12) emission enters ionization chamber (11), then by speculum () 13, vertically guide light into sample area, the reverberation of sample area is drawn light ionization chamber (11) and is arrived ccd image collector (15) acquisition interface through speculum (14), the sample image that ccd image collector (15) gathers by data importing computer (16), is seen the image of sample area by the USB line by computer (16).
7. device according to claim 6, wherein speculum (13) is arranged at the upper end of mass analyzer (9), the left side of ion beam, speculum (14) is positioned at the upper end of mass analyzer (9), the right side of ion beam.
8. device according to claim 7, wherein speculum (13), speculum (14) can be right-angle prism.
9. device according to claim 8, wherein ccd image collector (15) is arranged on a translation guide rail, is convenient to focusing and obtains sample area image clearly.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013202176729U CN203300593U (en) | 2013-04-25 | 2013-04-25 | Laser introducing and sample imaging apparatus for MALDI source |
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| CN2013202176729U CN203300593U (en) | 2013-04-25 | 2013-04-25 | Laser introducing and sample imaging apparatus for MALDI source |
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| CN203300593U true CN203300593U (en) | 2013-11-20 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104795306A (en) * | 2015-04-17 | 2015-07-22 | 江苏天瑞仪器股份有限公司 | Sample stimulating and sample imaging light path device for matrix assisted laser desorption ionization |
| CN105388301A (en) * | 2015-12-11 | 2016-03-09 | 安图实验仪器(郑州)有限公司 | Matrix assisted laser desorption ion source laser and imaging integrated system |
| CN109682880A (en) * | 2017-10-18 | 2019-04-26 | 株式会社岛津制作所 | Mass spectrometer apparatus for management of information |
| CN110349832A (en) * | 2019-06-21 | 2019-10-18 | 清华大学 | A kind of device to distort for correcting time of-flight mass spectrometer accelerating field |
| CN113834870A (en) * | 2021-08-27 | 2021-12-24 | 中国科学院大连化学物理研究所 | Laser resolution VUV lamp rear ionization imaging device under atmospheric pressure |
| CN113921368A (en) * | 2021-10-11 | 2022-01-11 | 南京谱联生命科学技术研究院有限公司 | Laser energy control method of time-of-flight mass spectrometer |
| CN114899079A (en) * | 2021-04-28 | 2022-08-12 | 中国科学院江西稀土研究院 | Mass spectrum ionization source of surface coupling induction plasma source and corresponding mass spectrometer |
-
2013
- 2013-04-25 CN CN2013202176729U patent/CN203300593U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104795306A (en) * | 2015-04-17 | 2015-07-22 | 江苏天瑞仪器股份有限公司 | Sample stimulating and sample imaging light path device for matrix assisted laser desorption ionization |
| CN105388301A (en) * | 2015-12-11 | 2016-03-09 | 安图实验仪器(郑州)有限公司 | Matrix assisted laser desorption ion source laser and imaging integrated system |
| CN109682880A (en) * | 2017-10-18 | 2019-04-26 | 株式会社岛津制作所 | Mass spectrometer apparatus for management of information |
| CN109682880B (en) * | 2017-10-18 | 2022-01-07 | 株式会社岛津制作所 | Information management device for mass spectrometer |
| CN110349832A (en) * | 2019-06-21 | 2019-10-18 | 清华大学 | A kind of device to distort for correcting time of-flight mass spectrometer accelerating field |
| CN114899079A (en) * | 2021-04-28 | 2022-08-12 | 中国科学院江西稀土研究院 | Mass spectrum ionization source of surface coupling induction plasma source and corresponding mass spectrometer |
| CN114899079B (en) * | 2021-04-28 | 2023-04-14 | 中国科学院江西稀土研究院 | Mass spectrum ionization source of surface coupling induction plasma source and corresponding mass spectrometer |
| CN113834870A (en) * | 2021-08-27 | 2021-12-24 | 中国科学院大连化学物理研究所 | Laser resolution VUV lamp rear ionization imaging device under atmospheric pressure |
| CN113921368A (en) * | 2021-10-11 | 2022-01-11 | 南京谱联生命科学技术研究院有限公司 | Laser energy control method of time-of-flight mass spectrometer |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
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Owner name: BEIJING YIXIN BOCHUANG BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: MA QINGWEI Effective date: 20150114 |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Free format text: CORRECT: ADDRESS; FROM: 100085 HAIDIAN, BEIJING TO: 100023 DAXING, BEIJING |
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Effective date of registration: 20150114 Address after: 100023 1 building, No. 1, East Sheng Road, Beijing economic and Technological Development Zone, Beijing, B201 Patentee after: BEIJING CLIN BOCHUANG BIOTECHNOLOGY Co.,Ltd. Address before: 100085 Beijing city Haidian District Road West of new Silicon Valley two xi'erqi Lingxiu Hospital No. 38 Building Room 102 Patentee before: Ma Qingwei |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131120 |
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| CF01 | Termination of patent right due to non-payment of annual fee |