CN201247221Y - Micro-fluidic chip capillary tube cataphoresis apparatus system used for multi-channel and multi-detection unitedly - Google Patents
Micro-fluidic chip capillary tube cataphoresis apparatus system used for multi-channel and multi-detection unitedly Download PDFInfo
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- CN201247221Y CN201247221Y CNU2008200467108U CN200820046710U CN201247221Y CN 201247221 Y CN201247221 Y CN 201247221Y CN U2008200467108 U CNU2008200467108 U CN U2008200467108U CN 200820046710 U CN200820046710 U CN 200820046710U CN 201247221 Y CN201247221 Y CN 201247221Y
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Abstract
The utility model relates to the field of an analyzing device of the electrophoretic separation of capillary tubes, in particular to an electrophoresis system of micro-fluidic chip capillary tubes, which aims at overcoming defects in the prior art, combines two kinds of detecting means, can simultaneously analyze the component of different nature in a complex system, improves the selectivity of the method, speeds up the analyzing speed, improves the working efficiency and jointly used with multi-channel and multi-detecting. The improved structure of the system at least comprises an optical detection channel and an electrochemical detection channel, the specific structure is that the optical detection channel and the electrochemical detection channel comprise quartz capillary tubes, microchip electrochemical detecting tanks which are arranged on the tail ends of the quartz capillary tubes, and optical detectors which are arranged on the rear portions of the quartz capillary tubes and at least one electrochemical detector which is connected with the microchip electrochemical detecting tanks. The optical detector and the electrochemical detector are connected with a data collecting device.
Description
Technical field
The utility model relates to capillary electrophoresis separation analytical equipment field, and a kind of hyperchannel of saying so more specifically detects the micro flow control chip capillary electrophoresis instrument system of coupling more.
Background technology
(Capillary Electrophoresis CE) is development in recent years one of compartment analysis means the most rapidly to Capillary Electrophoresis.It is that ion or charged particle are driving force with the electric field, carries out efficiently or/and partition factor is different by its mobility in quartz capillary, a kind of isolation technics fast.Efficiently (the plate theory number is greater than 10 because CE has
5/ rice), fast (analysis time was less than 30 minutes), sampling volume are little (is generally and receives upgrading-10
-9L), advantage such as solvent consumption is few and sample pretreatment is simple, since the eighties is come out, all be widely used in fields such as chemistry, environment, pharmacy, clinical, life sciences.
The exploitation of detecting device is one of key problem of CE development always.Detecting device commonly used among the CE comprises fluorescence detector and electrochemical detector.Ultraviolet absorption detector is the commodity capillary electrophoresis apparatus fluorescence detector of normal outfit, have the advantages that versatility is good, sensitivity is moderate, be applicable to the mensuration that the compound of absorption is arranged in the UV, visible light optical range, its shortcoming is that optical detection is subjected to the restriction that capillary inner diameter is little, light path is short because the mensuration of sample depends on the length of light path.It is that CE future is the most potential and make chip technology become possible a kind of detection technique that Electrochemical Detection is called, and is characterized in that instrument is simple, be easy to microminiaturization, cheap, is easy to popularize.It is good that electricity is wherein led the detection versatility, as long as the conductivity of measured matter and buffering electrical conductivity of solution have certain difference just can be detected, is applicable to the mensuration of various easy disassociation materials, and its shortcoming is that sensitivity is low; Amperometric Detection Coupled has highly sensitive (being one of the highest detecting device of CE medium sensitivity), and specificity, the advantage that detection volume is little can be used for the detection of individual cells, but it requires detected material to have electroactive group, and this has limited its range of application.
The utility model content
The purpose of this utility model is to overcome deficiency of the prior art, merge two kinds of detection meanss, has component of different nature in the Analysis of Complex system simultaneously, the selectivity of raising method, accelerate analysis speed, the hyperchannel of increasing work efficiency detects the micro flow control chip capillary electrophoresis instrument system of coupling more.
The utility model is achieved through the following technical solutions its purpose.
The utility model has designed a kind of multichannel micro-fluidic chip Capillary Electrophoresis instrument system, its structure-improved is to comprise optical detection passage and Electrochemical Detection passage at least, to be described optical detection passage be made up of quartz capillary, the microchip electrochemical investigating pond that is installed in the quartz capillary end, the fluorescence detector that is installed in the quartz capillary rear portion are connected the microchip electrochemical investigating pond with at least one electrochemical detector with the Electrochemical Detection passage concrete structure, and described fluorescence detector is connected with data acquisition equipment with electrochemical detector.Also comprise a high pressure generator, described microchip electrochemical investigating pond directly is connected with high pressure generator, and the quartz capillary front end is connected with high pressure generator by a Buffer Pool.This patent is the micro flow control chip capillary electrophoresis instrument system with hyperchannel-many detection techniques coupling of characteristics such as cheap, microminiaturized, integrated, high-performance.
The utility model adopts hyperchannel and the coupling of many detection techniques, and the microchip electrochemical investigating pond connects electrochemical detector, and described electrochemical detector is connected with data acquisition equipment respectively.The general data collecting card that is connected with computing machine that adopts of described data acquisition equipment also can be the data acquisition unit etc. of standalone feature.
This patent is a stock with cheap macromolecular material, glass and quartz capillary, adopt little processing of facture of microchip and quartz capillary coupling technique, fluorescence detector and electrochemical detector are connected in parallel in the same chip capillary cataphoresis piece-rate system, several method is replenished mutually, bring out one's strengths to make up for one's weaknesses, a sample introduction just can obtain all information of a plurality of detection compositions simultaneously.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
A kind of hyperchannel as shown in Figure 1 detects the micro flow control chip capillary electrophoresis instrument system of coupling more, main detection architecture comprises optical detection passage and Electrochemical Detection passage, described optical detection passage and Electrochemical Detection passage are made up of with the electrochemical detector 3 that is connected microchip electrochemical investigating pond 2 quartz capillary 1, the microchip electrochemical investigating pond 2 that is installed in quartz capillary 1 end, the fluorescence detector 4 that is installed in the quartz capillary rear portion, and described fluorescence detector 4 is connected with computing machine 6 by data collecting card 5 with electrochemical detector 3.
Above-mentioned hyperchannel detects the micro flow control chip capillary electrophoresis instrument system of coupling more, also comprise a high pressure generator 8, described microchip electrochemical investigating pond 2 directly is connected with high pressure generator 8, and quartz capillary 1 front end is connected with high pressure generator 8 by a Buffer Pool 7.
Above-mentioned design considers that the absorption cell of fluorescence detector 4 ultraviolet detection should adopt quartz material, and the difficulty of processing of ultraviolet detection system is bigger on the chip of quartz material, so the separation of sample is carried out in quartz capillary 1.On the other hand that electrochemical investigating pond 2 is microminiaturized, integrated as much as possible, so the design of total system is to adopt quartz capillary and microchip coupling technique.The utility model will separate from the sample that sample Buffer Pool 7 comes out, the optical detection part is carried out at quartz capillary 1, and the Electrochemical Detection part is carried out on the chip of microchip electrochemical investigating pond 2.Successively fluorescence detector 4, electrochemical detector 3 are placed on the rear portion and the end of quartz capillary 1, make it not disturb mutually.
The utility model adopts little processing of facture of microchip and quartz capillary coupling technique, fluorescence detector and electrochemical detector are connected in parallel in the same chip capillary cataphoresis piece-rate system, several method is replenished mutually, bring out one's strengths to make up for one's weaknesses, a sample introduction just can obtain all information of a plurality of detection compositions simultaneously.
Claims (3)
1. a hyperchannel detects the micro flow control chip capillary electrophoresis instrument system of coupling more, it is characterized in that comprising at least optical detection passage and Electrochemical Detection passage, described optical detection passage and Electrochemical Detection passage are made up of quartz capillary, the microchip electrochemical investigating pond that is installed in the quartz capillary end, the fluorescence detector that is installed in the quartz capillary rear portion are connected the microchip electrochemical investigating pond with at least one electrochemical detector, and described fluorescence detector is connected with data acquisition equipment with electrochemical detector.
2. hyperchannel according to claim 1 detects the micro flow control chip capillary electrophoresis instrument system of coupling more, it is characterized in that also comprising a high pressure generator, described microchip electrochemical investigating pond directly is connected with high pressure generator, and the quartz capillary front end is connected with high pressure generator by a Buffer Pool.
3. hyperchannel according to claim 1 and 2 detects the micro flow control chip capillary electrophoresis instrument system of coupling more, it is characterized in that the data collecting card of described data acquisition equipment for being connected with computing machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200467108U CN201247221Y (en) | 2008-04-22 | 2008-04-22 | Micro-fluidic chip capillary tube cataphoresis apparatus system used for multi-channel and multi-detection unitedly |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200467108U CN201247221Y (en) | 2008-04-22 | 2008-04-22 | Micro-fluidic chip capillary tube cataphoresis apparatus system used for multi-channel and multi-detection unitedly |
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| CN201247221Y true CN201247221Y (en) | 2009-05-27 |
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| CNU2008200467108U Expired - Fee Related CN201247221Y (en) | 2008-04-22 | 2008-04-22 | Micro-fluidic chip capillary tube cataphoresis apparatus system used for multi-channel and multi-detection unitedly |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614690B (en) * | 2009-07-27 | 2012-05-16 | 中国科学院长春应用化学研究所 | Multi-channel electrochemical analyzer |
| CN102539512A (en) * | 2012-02-10 | 2012-07-04 | 上海康盈生物科技有限公司 | Multichannel capillary electrophoresis method for detecting glycosylated hemoglobin HbA1c in multiple samples simultaneously |
-
2008
- 2008-04-22 CN CNU2008200467108U patent/CN201247221Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614690B (en) * | 2009-07-27 | 2012-05-16 | 中国科学院长春应用化学研究所 | Multi-channel electrochemical analyzer |
| CN102539512A (en) * | 2012-02-10 | 2012-07-04 | 上海康盈生物科技有限公司 | Multichannel capillary electrophoresis method for detecting glycosylated hemoglobin HbA1c in multiple samples simultaneously |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090527 Termination date: 20120422 |