CN204679421U - Fluorescence detection device - Google Patents
Fluorescence detection device Download PDFInfo
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- CN204679421U CN204679421U CN201520319714.9U CN201520319714U CN204679421U CN 204679421 U CN204679421 U CN 204679421U CN 201520319714 U CN201520319714 U CN 201520319714U CN 204679421 U CN204679421 U CN 204679421U
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- fluorescence
- exciting light
- dichroic mirror
- detection device
- reflective mirror
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Abstract
The utility model embodiment provides a kind of fluorescence detection device, and this fluorescence detection device comprises: excitation source launches exciting light; Exciting light is reflexed to microcobjective place by dichroic mirror; Microcobjective, for exciting light being focused on the fluorescence radiation point of detected sample, the fluorescence that optical excitation sends and the fluorescence radiation point gathering detected sample is excited, fluorescence is irradiated on reflective mirror through dichroic mirror; Reflective mirror is arranged on directly over dichroic mirror, and reflective mirror is identical with inclination angle that horizontal direction becomes with dichroic mirror with inclination angle that horizontal direction becomes, for by fluorescent reflection to the first photoelectric detector; The fluorescence received is converted to electric current by the first photoelectric detector, and electric current provides foundation for detecting the fluorescent intensity parameter of detected sample.The program can realize the feature that fluorescence detection device volume is little, cost is low, simple to operate, and this fluorescence detection device can meet the requirement of clinical quick detection fluorescence simultaneously.
Description
Technical field
The utility model relates to technical field of fluorescence detection, particularly a kind of fluorescence detection device.
Background technology
Fluorescence, is counted as the important tool in biology, clinical diagnosis and cytology research for a long time, and meanwhile, market is increasing for the demand of clinical quick detection fluorescence.The general volume of traditional fluorescence detector is large, price is high, complicated operation, is difficult to the requirement meeting clinical quick detection.
Utility model content
The utility model embodiment provides a kind of fluorescence detection device, and to realize the feature that fluorescence detection device volume is little, cost is low, simple to operate, this fluorescence detection device can meet the requirement of clinical quick detection fluorescence simultaneously.This device comprises: excitation source, dichroic mirror, microcobjective, reflective mirror and the first photoelectric detector, and wherein, described excitation source, for launching exciting light; Described dichroic mirror, for reflexing to described microcobjective place by described exciting light; Described microcobjective, for described exciting light being focused on the fluorescence radiation point of described detected sample, and the fluorescence radiation point gathering described detected sample the fluorescence that sends by described excitation, described fluorescence is irradiated on described reflective mirror through described dichroic mirror; Described reflective mirror, described reflective mirror is arranged on directly over described dichroic mirror, and described reflective mirror is identical with inclination angle that horizontal direction becomes with described dichroic mirror with inclination angle that horizontal direction becomes, for by described fluorescent reflection to described first photoelectric detector; Described first photoelectric detector, for the fluorescence received is converted to electric current, described electric current provides foundation for the fluorescent intensity parameter detecting described detected sample.
In one embodiment, also comprise: exciting light optical filter, be arranged between described excitation source and described dichroic mirror; First lens, are arranged between described exciting light optical filter and described dichroic mirror; Utilizing emitted light optical filter, is arranged between described reflective mirror and described first photoelectric detector; Second lens, are arranged between described reflective mirror and described utilizing emitted light optical filter.
In one embodiment, also comprise: communication interface, for the fluorescent intensity parameter of described detected sample is sent to external unit.
In one embodiment, also comprise: temperature sensor, for detecting the temperature of described excitation source, the exciting light that this temperature is used for launching for adjusting described excitation source preset strength provides foundation; Second photoelectric detector, for detecting the intensity of the exciting light that described excitation source is launched, the exciting light that the intensity of this exciting light is used for launching for adjusting described excitation source preset strength provides foundation.
In one embodiment, also comprise: main body, for carrying all parts that fluorescence detection device comprises.
In one embodiment, all parts that described fluorescence detection device comprises is installed on the body by pilot hole.
In the utility model embodiment, by integrated excitation source, can directly the fluorescence radiation of detected sample point not be excited to send fluorescence by other equipment, and by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, to detect the fluorescent intensity of detected sample, fluorescence detection device is made to can be used alone to detect the fluorescent intensity of detected sample, compared with the large-scale fluorescence detector of tradition, while meeting the requirement of clinical quick detection fluorescence, fluoroscopic examination operation can be simplified; In addition, due to by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, make fluorescence detection device compact conformation, reduce the volume of fluorescence detection device, cost is low, strong adaptability.
Accompanying drawing explanation
Accompanying drawing described herein is used to provide further understanding of the present utility model, forms a application's part, does not form restriction of the present utility model.In the accompanying drawings:
Fig. 1 is the cut-away view of a kind of fluorescence detection device that the utility model embodiment provides;
Fig. 2 is the schematic appearance of a kind of fluorescence detection device that the utility model embodiment provides;
Fig. 3 is the assembling schematic diagram of a kind of fluorescence detection device that the utility model embodiment provides.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with embodiment and accompanying drawing, the utility model is described in further details.At this, exemplary embodiment of the present utility model and illustrating for explaining the utility model, but not as to restriction of the present utility model.
In the utility model embodiment, provide a kind of fluorescence detection device, as shown in Figure 1, this fluorescence detection device comprises: excitation source 101, dichroic mirror 102, microcobjective 103, reflective mirror 104 and the first photoelectric detector 105, wherein,
Described excitation source 101, for launching exciting light; θ is the lighting angle of excitation source 101;
Described dichroic mirror 102, for reflexing to described microcobjective place by described exciting light;
Described microcobjective 103, for described exciting light being focused on the fluorescence radiation point of described detected sample, and the fluorescence radiation point gathering described detected sample the fluorescence that sends by described excitation, described fluorescence is irradiated on described reflective mirror through described dichroic mirror;
Described reflective mirror 104, described reflective mirror is arranged on directly over described dichroic mirror, and described reflective mirror is identical with inclination angle that horizontal direction becomes with described dichroic mirror with inclination angle that horizontal direction becomes, for by described fluorescent reflection to described first photoelectric detector;
Described first photoelectric detector 105, for the fluorescence received is converted to electric current 113, described electric current provides foundation for the fluorescent intensity parameter detecting described detected sample.
Known as shown in Figure 1, in the utility model embodiment, by integrated excitation source (such as, LED light source), can directly the fluorescence radiation of detected sample point not be excited to send fluorescence by other equipment, and by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, to detect the fluorescent intensity of detected sample, fluorescence detection device is made to can be used alone to detect the fluorescent intensity of detected sample, compared with the large-scale fluorescence detector of tradition, while meeting the requirement of clinical quick detection fluorescence, fluoroscopic examination operation can be simplified, in addition, due to by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, make fluorescence detection device compact conformation, reduce the volume of fluorescence detection device, cost is low, strong adaptability.
During concrete enforcement, after the fluorescence received is converted to electric current by the first photoelectric detector 105, single-chip microcomputer can be adopted with above-mentioned electric current for obtaining the fluorescent intensity parameter of detected sample according to detection.
During concrete enforcement, as shown in Figure 1, above-mentioned fluorescence detection device also comprises: exciting light optical filter 107, is arranged on (as shown in Figure 1, d is the focal length between exciting light optical filter 107 and excitation source 101) between described excitation source 101 and described dichroic mirror 102; First lens 108, are arranged between described exciting light optical filter 107 and described dichroic mirror 102; Utilizing emitted light optical filter 109, is arranged between described reflective mirror 104 and described first photoelectric detector 105; Second lens 110, are arranged between described reflective mirror 104 and described utilizing emitted light optical filter 109.Known as shown in Figure 1, the light path of exciting light is successively by focusing on the fluorescence radiation point of detected sample 106 after exciting light optical filter 107, first lens 108, dichroic mirror 102 and microcobjective 103, the light path of fluorescence is successively by microcobjective 103, through dichroic mirror 102, reflect through reflective mirror 104, focus on the receiving plane of the first photoelectric detector 105 after second lens 110 and utilizing emitted light optical filter 109, namely the light path of exciting light and the light path of fluorescence constitute common focusing structure, in this common focusing structure, exciting light and fluorescence share the light path between microcobjective 103 to dichroic mirror 102, also because dichroic mirror 102 and the alignment of reflective mirror 104 in the vertical direction arrange (namely reflective mirror 104 is arranged on directly over dichroic mirror 102) and the two is identical with the inclination angle of horizontal direction, namely reflected at reflective mirror 104 place fluorescence, by the fluorescence after reflecting above exciting light and with the light ray parallel of exciting light, make the more compact structure of fluorescence detection device, strong adaptability, the fluorescence simultaneously only having the sample at focal beam spot place to send could arrive detection window, the receiving plane of the first photoelectric detector 105 in this detection window corresponding diagram 1, and other position nonspecific signals is optionally shielded, make to affect by external stray light less, improve accuracy in detection.
During concrete enforcement, in order to improve the use dirigibility of above-mentioned fluorescence detection device, in the present embodiment, above-mentioned fluorescence detection device, also comprises: communication interface, for the fluorescent intensity parameter of described detected sample is sent to external unit.Such as, this communication interface can be RS485.Concrete, when adopting single-chip microcomputer to detect the fluorescent intensity parameter of detected sample with above-mentioned electric current for foundation, as shown in Figure 2, above-mentioned communication interface and single-chip microcomputer are (such as, this single-chip microcomputer can be C8051F020) can be arranged on circuit board 201, after the fluorescence received is converted to electric current by the first photoelectric detector 105, the current detection circuit that this electric current can enter circuit board carries out AD conversion (analog to digital conversion), electric current after AD conversion enters above-mentioned single-chip microcomputer, this single-chip microcomputer is according to detecting with electric current, and export the fluorescent intensity parameter corresponding with electric current, by the communication interface on circuit board, fluorescent intensity parameter is sent to external unit again.
During concrete enforcement, in order to ensure the accuracy detected, excitation source is needed to keep sending the exciting light (exciting light required during detection fluorescence) of preset strength, in the present embodiment, above-mentioned fluorescence detection device, also comprise: temperature sensor 111, for detecting the temperature of described excitation source, the exciting light that this temperature is used for launching for adjusting described excitation source preset strength provides foundation; Second photoelectric detector 112, for detecting the intensity of the exciting light that described excitation source is launched, the exciting light that the intensity of this exciting light is used for launching for adjusting described excitation source preset strength provides foundation.Concrete, the intensity of the exciting light that single-chip microcomputer can be adopted to launch with the temperature of described excitation source and described excitation source, for foundation, adjusts the exciting light that described excitation source launches preset strength.
During concrete enforcement, the conveniently use of above-mentioned fluorescence detection device, in the present embodiment, as shown in Figure 2, fluorescence detection device also comprises: main body 202, for carrying all parts that fluorescence detection device comprises.Such as, the parts such as excitation source 101, dichroic mirror 102, microcobjective 103, reflective mirror 104, first photoelectric detector 105, exciting light optical filter 107, first lens 108, utilizing emitted light optical filter 109 and second lens 110 of comprising of fluorescence detection device all can be arranged in aforementioned body 202.
Concrete, in order to adjust and change parts, as shown in Figure 3, the all parts that described fluorescence detection device comprises is arranged in described main body 202 by pilot hole, such as, the excitation source 101 that fluorescence detection device comprises, dichroic mirror 102, microcobjective 103, reflective mirror 104, first photoelectric detector 105, exciting light optical filter 107, first lens 108, the parts such as utilizing emitted light optical filter 109 and the second lens 110 all can be arranged in aforementioned body 202, wherein, first side panel 303 of main body 202 and the second side panel 304 are used for fixing dichroic mirror 102 and reflective mirror 104, circuit board 201 is fixed on the first side panel 303 or the second side panel 304, fabrication hole in main body 202 is configured with fabrication hole cover plate 305, microcobjective 103 comprises diaphragm 302 and the 3rd lens 301.
In the utility model embodiment, by integrated excitation source, can directly the fluorescence radiation of detected sample point not be excited to send fluorescence by other equipment, and by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, to detect the fluorescent intensity of detected sample, fluorescence detection device is made to can be used alone to detect the fluorescent intensity of detected sample, compared with the large-scale fluorescence detector of tradition, while meeting the requirement of clinical quick detection fluorescence, fluoroscopic examination operation can be simplified; In addition, due to by the reflective mirror identical with dichroic mirror setting direction by fluorescent reflection to the first photoelectric detector, make fluorescence detection device compact conformation, reduce the volume of fluorescence detection device, cost is low, strong adaptability.
The foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, for a person skilled in the art, the utility model embodiment can have various modifications and variations.All within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (6)
1. a fluorescence detection device, is characterized in that, comprising: excitation source, dichroic mirror, microcobjective, reflective mirror and the first photoelectric detector, wherein,
Described excitation source, for launching exciting light;
Described dichroic mirror, for reflexing to described microcobjective place by described exciting light;
Described microcobjective, for described exciting light being focused on the fluorescence radiation point of described detected sample, and the fluorescence radiation point gathering described detected sample the fluorescence that sends by described excitation, described fluorescence is irradiated on described reflective mirror through described dichroic mirror;
Described reflective mirror, described reflective mirror is arranged on directly over described dichroic mirror, and described reflective mirror is identical with inclination angle that horizontal direction becomes with described dichroic mirror with inclination angle that horizontal direction becomes, for by described fluorescent reflection to described first photoelectric detector;
Described first photoelectric detector, for the fluorescence received is converted to electric current, described electric current provides foundation for the fluorescent intensity parameter detecting described detected sample.
2. device as claimed in claim 1, is characterized in that, also comprise:
Exciting light optical filter, is arranged between described excitation source and described dichroic mirror;
First lens, are arranged between described exciting light optical filter and described dichroic mirror;
Utilizing emitted light optical filter, is arranged between described reflective mirror and described first photoelectric detector;
Second lens, are arranged between described reflective mirror and described utilizing emitted light optical filter.
3. device as claimed in claim 1, is characterized in that, also comprise:
Communication interface, for sending to external unit by the fluorescent intensity parameter of described detected sample.
4. device as claimed in claim 1, is characterized in that, also comprise:
Temperature sensor, for detecting the temperature of described excitation source, the exciting light that this temperature is used for launching for adjusting described excitation source preset strength provides foundation;
Second photoelectric detector, for detecting the intensity of the exciting light that described excitation source is launched, the exciting light that the intensity of this exciting light is used for launching for adjusting described excitation source preset strength provides foundation.
5. the device according to any one of Claims 1-4, is characterized in that, also comprises:
Main body, for carrying all parts that fluorescence detection device comprises.
6. device as claimed in claim 5, it is characterized in that, all parts that described fluorescence detection device comprises is installed on the body by pilot hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520319714.9U CN204679421U (en) | 2015-05-18 | 2015-05-18 | Fluorescence detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520319714.9U CN204679421U (en) | 2015-05-18 | 2015-05-18 | Fluorescence detection device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105759025A (en) * | 2016-03-01 | 2016-07-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Photoelectric detection system and time-resolved fluorescence immunoassay analyzer |
| CN106770092A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of hand-held fluorescence fast detector |
| CN112683869A (en) * | 2020-12-25 | 2021-04-20 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN112945921A (en) * | 2021-02-01 | 2021-06-11 | 广州市鹏达智能科技有限公司 | Fluorescence quantitative analysis device and method |
-
2015
- 2015-05-18 CN CN201520319714.9U patent/CN204679421U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105759025A (en) * | 2016-03-01 | 2016-07-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Photoelectric detection system and time-resolved fluorescence immunoassay analyzer |
| CN106770092A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of hand-held fluorescence fast detector |
| CN112683869A (en) * | 2020-12-25 | 2021-04-20 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN112683869B (en) * | 2020-12-25 | 2023-03-14 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN112945921A (en) * | 2021-02-01 | 2021-06-11 | 广州市鹏达智能科技有限公司 | Fluorescence quantitative analysis device and method |
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