CN112945921A - Fluorescence quantitative analysis device and method - Google Patents
Fluorescence quantitative analysis device and method Download PDFInfo
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- CN112945921A CN112945921A CN202110138603.8A CN202110138603A CN112945921A CN 112945921 A CN112945921 A CN 112945921A CN 202110138603 A CN202110138603 A CN 202110138603A CN 112945921 A CN112945921 A CN 112945921A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The present application provides a fluorescent quantitative analysis device, the device comprising: the device comprises an excitation light source, a receiver, an excitation light filter and an emission filter; the exciting light filter is positioned between the excitation light source and the motion trail of the detected target object, and the exciting light emitted by the excitation light source passes through the exciting light filter; the emission filter is positioned between the receiver and the motion track of the detected target object, and the emission light received by the receiver passes through the emission filter. Therefore, the fluorescent quantitative analysis device can effectively avoid the problems of dichromatic mirror reflection and transmission efficiency, substrate autofluorescence of the detected target object and interference of other fluorescent substances in the detected target object, thereby improving the accuracy of fluorescent quantitative detection.
Description
Technical Field
The application relates to the field of fluorescence detection, in particular to a fluorescence quantitative analysis device and method.
Background
The current fluorescence quantitative analysis device adopts an excitation light source to irradiate on a detected target object with fluorescein, the fluorescein generates emission light with different wavelengths from the excitation light source, the emission light is received by a receiver through an emission light filter, and the receiver can deduce the content of the detected target object according to quantitative analysis of the emission light because the intensity of the emission light is strictly and positively correlated with the intensity of the emission light. As shown in fig. 1, in this conventional method, the excitation light source and the emission light are arranged vertically, and vertically hit on the target object to be detected after being semi-reflected by the dichroic mirror, so as to form a detection environment in which the excitation light and the emission light are coaxial in fact; when the emission light is detected, the excitation light is coaxial with the emission light, the excitation light and the emission light are separated by a filter, and the emission light is quantitatively analyzed.
However, this conventional method has problems of emission efficiency of the dichroic mirror for excitation light and transmission efficiency for emission light; there is also a problem that since the excitation light is coaxial with the emission light, the excitation light interferes with the detection of the emission light; in addition, when the excitation light coaxially hits the target object to be detected, the autofluorescence of the target object bearing substrate and the fluorescence of other substances in the target object to be detected are mixed in the emitted light to interfere with quantitative detection. Therefore, a solution capable of reducing interference of background and other luminescent substances in the reactant to the fluorescence quantitative analysis in the fluorescence detection process and improving the accuracy of the fluorescence quantitative analysis is needed.
Disclosure of Invention
The application provides a fluorescence quantitative analysis device to make the fluorescence quantitative analysis device of this application can effectively avoid dichromatic mirror reflection and transmission efficiency, the base plate of being detected the target object from fluorescence, be detected the problem that other fluorescent material disturbed in the target object, thereby improve fluorescence quantitative determination's accuracy.
In a first aspect, the present application provides a fluorescent quantitative analysis device, the device comprising: the device comprises an excitation light source, a receiver, an excitation light filter and an emission filter;
the excitation light filter is positioned between the excitation light source and the motion trail of the detected target object, and the excitation light emitted by the excitation light source passes through the excitation light filter;
the emission filter is positioned between the receiver and the motion trail of the detected target object, and the emission light received by the receiver passes through the emission filter;
the excitation light source is used for responding to an emission instruction and emitting excitation light;
the receiver is used for receiving the emitted light emitted by the detected target object and quantitatively analyzing the fluorescein in the detected target object according to the emitted light.
In a second aspect, the present application provides a fluorescent quantitative analysis method applied to the fluorescent quantitative analysis device according to any one of the first aspect, the method including:
responding to an emission instruction, controlling an excitation light source to emit excitation light, and irradiating the excitation light to a detected target object through an excitation light filter;
controlling the detected target object to move along the target movement direction at a preset speed;
if the detected target object moves to the position corresponding to the receiver, controlling the receiver to receive the emitted light which is emitted by the detected target object and passes through the emission filter;
and carrying out quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
In a third aspect, the present application provides a fluorescent quantitative analysis device, the device comprising:
the first control unit is used for responding to an emission instruction and controlling the excitation light source to emit excitation light, and the excitation light irradiates the detected target object through the excitation light filter;
the second control unit is used for controlling the detected target object to move along the target movement direction at a preset speed;
a third control unit, configured to control the receiver to receive the emitted light that is emitted by the detected object and passes through the emission filter if the detected object moves to a position corresponding to the receiver;
and the analysis unit is used for carrying out quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
In a fourth aspect, the present application provides a readable medium comprising executable instructions, which when executed by a processor of an electronic device, cause the electronic device to perform the method according to any of the second aspects.
In a fifth aspect, the present application provides an electronic device comprising a processor and a memory storing execution instructions, wherein when the processor executes the execution instructions stored in the memory, the processor performs the method according to any one of the second aspects.
As can be seen from the above technical solutions, the present application provides a fluorescence quantitative analysis apparatus, including: the device comprises an excitation light source, a receiver, an excitation light filter and an emission filter; the excitation light filter is positioned between the excitation light source and the motion trail of the detected target object, and the excitation light emitted by the excitation light source passes through the excitation light filter; the emission filter is positioned between the receiver and the motion trail of the detected target object, and the emission light received by the receiver passes through the emission filter; the excitation light source is used for responding to an emission instruction and emitting excitation light; the receiver is used for receiving the emitted light emitted by the detected target object and quantitatively analyzing the fluorescein in the detected target object according to the emitted light. Compared with the prior art, because the fluorescent quantitative analysis device in the application can realize the fluorescent quantitative analysis without the dichroic mirror in the fluorescent quantitative analysis process, the fluorescent quantitative analysis device in the application cancels the dichroic mirror, thus effectively avoiding the problems of dichroic mirror reflection and transmission efficiency, substrate autofluorescence of the detected target object and interference of other fluorescent substances in the detected target object, and improving the accuracy of the fluorescent quantitative detection.
Further effects of the above-mentioned unconventional preferred modes will be described below in conjunction with specific embodiments.
Drawings
In order to more clearly illustrate the embodiments or prior art solutions of the present application, the drawings needed for describing the embodiments or prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and that other drawings can be obtained by those skilled in the art without inventive exercise.
FIG. 1 is a schematic view of a prior art fluorescence quantitative analysis apparatus;
FIG. 2 is a schematic structural diagram of a fluorescence quantitative analysis apparatus according to an embodiment of the present application;
FIG. 3 is a schematic flow chart of a fluorescence quantitative analysis method according to an embodiment of the present application;
FIG. 4 is a schematic structural diagram of a fluorescence quantitative analysis apparatus according to an embodiment of the present application;
fig. 5 is a schematic structural diagram of an electronic device according to an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the technical solutions of the present application will be described in detail and completely with reference to the following embodiments and accompanying drawings. It should be apparent that the described embodiments are only some of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Various non-limiting embodiments of the present application are described in detail below with reference to the accompanying drawings.
Referring to fig. 2, a fluorescence quantitative analysis device in an embodiment of the present application is shown. In this embodiment, the fluorescence quantitative analysis apparatus includes: excitation light source, receiver, excitation light filter and emission filter.
In one implementation manner of this embodiment, the excitation light source and the receiver may be disposed on the same side of the motion trajectory of the detected object. In an implementation manner, the moving direction of the moving track of the target object is a direction from the excitation light source to the receiver, that is, the target object moves from the excitation light source to the receiver, that is, the target object passes through the excitation light source and then passes through the receiver. In one implementation, the target object to be detected moves along the target moving direction at a preset speed in the same direction, for example, the moving speed of the target object to be detected is set to be between 0-2m/s, the target moving direction may be the direction from the excitation light source to the receiver, in this case, the moving track of the target object to be detected is a straight line, and the excitation light source and the receiver may be disposed on the upper side or the lower side of the moving track of the target object to be detected.
In an implementation manner of this embodiment, the excitation light emitted by the excitation light source and the emission light received by the receiver may be two parallel light beams. Specifically, the movement direction of the excitation light emitted by the excitation light source may be perpendicular to the movement track of the detected target object; and/or the moving direction of the emitted light received by the receiver can be vertical to the moving track of the detected target object. It should be noted that, in one implementation, the moving direction of the excitation light emitted by the excitation light source and the moving direction of the emission light received by the receiver may be opposite. Like this, compare with prior art, because this application sets up excitation light source and emission photoreceiver in the same one side of being detected the motion trail of target object, just the excitation light that the excitation light source launched with the emission light that the receiver received is two bundles of light that are parallel, so fluorescence quantitative analysis device in this application does not need the dichroscope, can effectively avoid dichroscope reflection and transmission efficiency like this, the base plate of being detected the target object from fluorescence, the problem of being detected other fluorescent material interferences in the target object to improve fluorescence quantitative determination's accuracy. It should be noted that, in other implementation manners of the present embodiment, the excitation light emitted by the excitation light source and the emission light received by the receiver may be two non-parallel light beams, for example, there may be an included angle between the excitation light and the emission light, for example, two non-parallel light beams (for example, two non-perpendicular light beams or two perpendicular light beams) may be provided.
In one implementation, the excitation light source may be disposed in parallel with the receiver. It is understood that the vertical distances between the excitation light source and the receiver and the motion trail of the detected target object are the same.
In one implementation, the excitation light source and the receiver are separated by a preset distance, and the preset distance is determined according to the fluorescence duration of the detected target object. It is emphasized that the preset distance between the excitation light source and the receiver needs to be ensured, and the time for moving the detected target object from the position corresponding to the excitation light source to the position corresponding to the receiver is less than the fluorescence duration time of the detected target object, so that the problem of interference caused by coaxiality of excitation light and emission light in the prior art can be effectively avoided.
In this embodiment, the excitation light filter is located between the excitation light source and the motion track of the target object to be detected, and the excitation light emitted by the excitation light source passes through the excitation light filter, specifically, the excitation light filter may be perpendicular to the excitation light source, and the excitation light filter is located between the excitation light source and the motion track of the target object to be detected. The emission filter is located between the receiver and the motion track of the detected target object, and the emission light received by the receiver passes through the emission filter, specifically, the emission filter may be perpendicular to the receiver, and the emission filter is located between the receiver and the motion track of the detected target object. In an implementation manner, the excitation light filter and the emission filter may be disposed in parallel, and it is understood that the vertical distances between the excitation light filter and the emission filter and the motion trajectory of the target object to be detected are the same.
In this embodiment, the excitation light source may be configured to emit excitation light in response to the emission instruction, that is, the excitation light source may emit the excitation light when receiving the emission instruction, so that the target object to be detected can receive the excitation light for irradiation.
The receiver is used for receiving the emitted light emitted by the detected target object and quantitatively analyzing the fluorescein in the detected target object according to the emitted light. Since the fluorescein can continuously generate fluorescence of a certain time T1 after being irradiated by the excitation light (typically, the time is ms level, depending on the fluorescein material), and the non-fluorescein does not generate fluorescence when not being irradiated by the excitation light, after the detected target object can receive the excitation light, when the detected target object moves to a position corresponding to the receiver, the receiver can receive the emitted light generated by the detected target object, and the receiver can receive the emitted light emitted by the detected target object, and perform quantitative analysis on the fluorescein in the detected target object according to the emitted light. Therefore, the fluorescent quantitative analysis device provided by the application cancels a dichroic mirror, an excitation light source and a transmission light receiving channel (namely a receiver) are vertically placed and staggered by a short distance, the relation between potential difference time and fluorescein fluorescence duration time is reasonably arranged, the problems of dichromatic reflection and transmission efficiency, autofluorescence of a detected target object substrate and interference of other fluorescent substances in the detected target object can be effectively avoided, the accuracy of fluorescent quantitative detection is improved, the original noise of a signal detected by the receiver is reduced, and the correlation between the light intensity and the concentration of the detected target object is better during quantitative analysis. The detection target substrate is a substrate on which an immunoreaction is carried out by carrying fluorescein, that is, a substance having fluorescein, and can perform an immunoreaction on the substrate.
As can be seen from the above technical solutions, the present application provides a fluorescence quantitative analysis apparatus, including: the device comprises an excitation light source, a receiver, an excitation light filter and an emission filter; the excitation light source and the receiver are arranged on the same side of the motion track of the detected target object, and the excitation light emitted by the excitation light source and the emission light received by the receiver are two parallel light beams; the excitation light filter is positioned between the excitation light source and the motion trail of the detected target object, and the excitation light emitted by the excitation light source passes through the excitation light filter; the emission filter is positioned between the receiver and the motion trail of the detected target object, and the emission light received by the receiver passes through the emission filter; the excitation light source is used for responding to an emission instruction and emitting excitation light; the receiver is used for receiving the emitted light emitted by the detected target object and quantitatively analyzing the fluorescein in the detected target object according to the emitted light. Compared with the prior art, the fluorescent quantitative analysis device in the application cancels a dichroic mirror, and arranges the excitation light source and the emission light receiver at the same side of the motion track of the detected target object, and the excitation light emitted by the excitation light source and the emission light received by the receiver are two parallel beams of light, so that the problems of dichromatic mirror reflection and transmission efficiency, substrate autofluorescence of the detected target object and interference of other fluorescent substances in the detected target object can be effectively avoided, and the accuracy of fluorescent quantitative detection is improved.
Accordingly, the present embodiment also provides a method for using the fluorescence quantitative analysis device, namely a fluorescence quantitative analysis method applied to the fluorescence quantitative analysis device mentioned above, for the fluorescence quantitative analysis device corresponding to fig. 2. As shown in fig. 3, the method includes:
s301: and responding to the emission instruction, controlling the excitation light source to emit excitation light, and irradiating the excitation light to the detected target object through the excitation light filter.
In this embodiment, when a user performs an emission operation (for example, triggers a preset button), an emission instruction may be generated, and then, in response to the emission instruction, the excitation light source may be started and controlled to emit excitation light, so that the excitation light may be irradiated to the target object to be detected through the excitation light filter, and the fluorescein in the target object to be detected may continue to generate fluorescence for a certain time after being irradiated by the excitation light.
S302: and controlling the detected target object to move along the target movement direction at a preset speed.
And after the detected target object is irradiated by the exciting light, controlling the detected target object to move along the target movement direction at a preset speed. Wherein the target moving direction is a direction from the excitation light source to the receiver.
In one implementation, the target object to be detected moves along the target moving direction at a preset speed in the same direction, for example, the moving speed of the target object to be detected is set to be between 0-2m/s, in this case, the moving track of the target object to be detected is a straight line, and the excitation light source and the receiver may be disposed on the upper side or the lower side of the moving track of the target object to be detected.
S303: if the detected target object moves to the position corresponding to the receiver, controlling the receiver to receive the emitted light which is emitted by the detected target object and passes through the emission filter;
s304: and carrying out quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
When the target object moves to a position corresponding to the receiver, for example, as shown in fig. 2, when the receiver is located on the upper side of the target object, if the target object moves to a position right below the receiver, the receiver may receive the emitted light emitted by the target object and passing through the emission filter. Thus, the receiver can receive the emitted light generated by the detected target object, namely, the receiver can receive the emitted light emitted by the detected target object and carry out quantitative analysis on the fluorescein in the detected target object according to the emitted light. Therefore, the fluorescent quantitative analysis device provided by the application cancels a dichroic mirror, an excitation light source and a transmission light receiving channel (namely a receiver) are vertically placed and staggered by a short distance, the relation between potential difference time and fluorescein fluorescence duration time is reasonably arranged, the problems of dichromatic reflection and transmission efficiency, autofluorescence of a detected target object substrate and interference of other fluorescent substances in the detected target object can be effectively avoided, the accuracy of fluorescent quantitative detection is improved, the original noise of a signal detected by the receiver is reduced, and the correlation between the light intensity and the concentration of the detected target object is better during quantitative analysis. The detection target substrate is a substrate on which an immunoreaction is carried out by carrying fluorescein, that is, a substance having fluorescein, and can perform an immunoreaction on the substrate.
In an implementation manner of this embodiment, the fluorescence quantitative analysis apparatus may further include a processor, and the processor may perform quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
In one implementation, the quantitative analysis of fluorescein in the detected target according to the emitted light received by the receiver may include:
and determining the quantity of the fluorescein in the detected target object according to the preset speed, the preset distance between the excitation light source and the receiver and the light intensity of the emitted light.
Specifically, the fluorescence intensity attenuation amount of the fluorescein in the detected target object can be calculated according to a preset speed and a preset distance between the excitation light source and the receiver, and then the number of the fluorescein in the detected target object is determined according to the fluorescence intensity attenuation amount of the fluorescein in the detected target object and the light intensity of the emitted light.
In an implementation manner of this embodiment, the excitation light source and the receiver are separated by a predetermined distance, and the predetermined distance is determined according to the fluorescence duration of the detected target.
As can be seen from the above technical solutions, the present application provides a fluorescence quantitative analysis method, including: responding to an emission instruction, controlling an excitation light source to emit excitation light, and irradiating the excitation light to a detected target object through an excitation light filter; controlling the detected target object to move along the target movement direction at a preset speed; if the detected target object moves to the position corresponding to the receiver, controlling the receiver to receive the emitted light which is emitted by the detected target object and passes through the emission filter; and carrying out quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver. Compared with the prior art, the fluorescent quantitative analysis method in the application cancels a dichroic mirror, and arranges the excitation light source and the emission light receiver at the same side of the motion track of the detected target object, and the excitation light emitted by the excitation light source and the emission light received by the receiver are two parallel beams of light, so that the problems of dichromatic mirror reflection and transmission efficiency, substrate autofluorescence of the detected target object and interference of other fluorescent substances in the detected target object can be effectively avoided, and the accuracy of fluorescent quantitative detection is improved.
FIG. 4 shows an embodiment of a fluorescence quantitative analysis apparatus according to the present application. The device of this embodiment is a physical device for performing the fluorescence quantitative analysis method of the above embodiment. The technical solution is essentially the same as that in the above embodiment, and the corresponding description in the above embodiment is also applicable to this embodiment. The fluorescence quantitative analysis apparatus in this embodiment includes:
a first control unit 401, configured to control the excitation light source to emit excitation light in response to an emission instruction, where the excitation light is irradiated to the target object to be detected through the excitation light filter;
a second control unit 402, configured to control the detected target object to move along a target movement direction at a preset speed;
a third control unit 403, configured to control the receiver to receive the emitted light emitted by the detected object and passing through the emission filter if the detected object moves to a position corresponding to the receiver;
an analysis unit 404, configured to perform a quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
Optionally, the target moving direction is a direction from the excitation light source to the receiver.
Optionally, the analysis unit is specifically configured to:
and determining the quantity of the fluorescein in the detected target object according to the preset speed, the preset distance between the excitation light source and the receiver and the light intensity of the emitted light.
Fig. 5 is a schematic structural diagram of an electronic device according to an embodiment of the present application. On the hardware level, the electronic device comprises a processor and optionally an internal bus, a network interface and a memory. The Memory may include a Memory, such as a Random-Access Memory (RAM), and may further include a non-volatile Memory, such as at least 1 disk Memory. Of course, the electronic device may also include hardware required for other services.
The processor, the network interface, and the memory may be connected to each other via an internal bus, which may be an ISA (Industry Standard Architecture) bus, a PCI (Peripheral Component Interconnect) bus, an EISA (Extended Industry Standard Architecture) bus, or the like. The bus may be divided into an address bus, a data bus, a control bus, etc. For ease of illustration, only one double-headed arrow is shown in FIG. 5, but this does not indicate only one bus or one type of bus.
And the memory is used for storing the execution instruction. In particular, a computer program that can be executed by executing instructions. The memory may include both memory and non-volatile storage and provides execution instructions and data to the processor.
In a possible implementation manner, the processor reads the corresponding execution instruction from the nonvolatile memory to the memory and then runs the corresponding execution instruction, and the corresponding execution instruction can also be obtained from other equipment so as to form the fluorescence quantitative analysis device on a logic level. The processor executes the execution instructions stored in the memory to implement the fluorescence quantitative analysis method provided in any embodiment of the present application through the executed execution instructions.
The method performed by the fluorescence quantitative analysis apparatus according to the embodiment shown in fig. 3 of the present application can be applied to or implemented by a processor. The processor may be an integrated circuit chip having signal processing capabilities. In implementation, the steps of the above method may be performed by integrated logic circuits of hardware in a processor or instructions in the form of software. The Processor may be a general-purpose Processor, including a Central Processing Unit (CPU), a Network Processor (NP), and the like; but also Digital Signal Processors (DSPs), Application Specific Integrated Circuits (ASICs), Field Programmable Gate Arrays (FPGAs) or other Programmable logic devices, discrete Gate or transistor logic devices, discrete hardware components. The various methods, steps, and logic blocks disclosed in the embodiments of the present application may be implemented or performed. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like.
The steps of the method disclosed in connection with the embodiments of the present application may be directly implemented by a hardware decoding processor, or implemented by a combination of hardware and software modules in the decoding processor. The software module may be located in ram, flash memory, rom, prom, or eprom, registers, etc. storage media as is well known in the art. The storage medium is located in a memory, and a processor reads information in the memory and completes the steps of the method in combination with hardware of the processor.
The embodiment of the present application also provides a readable storage medium, which stores an execution instruction, and when the stored execution instruction is executed by a processor of an electronic device, the electronic device can be caused to execute the fluorescence quantitative analysis method provided in any embodiment of the present application, and is specifically used for executing the fluorescence quantitative analysis method.
The electronic device described in the foregoing embodiments may be a computer.
It will be apparent to those skilled in the art that embodiments of the present application may be provided as a method or computer program product. Accordingly, the present application may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects.
The embodiments in the present application are described in a progressive manner, and the same and similar parts among the embodiments can be referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, as for the apparatus embodiment, since it is substantially similar to the method embodiment, the description is relatively simple, and for the relevant points, reference may be made to the partial description of the method embodiment.
It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (10)
1. A fluorescent quantitative analysis device, characterized in that the device comprises: the device comprises an excitation light source, a receiver, an excitation light filter and an emission filter;
the excitation light filter is positioned between the excitation light source and the motion trail of the detected target object, and the excitation light emitted by the excitation light source passes through the excitation light filter;
the emission filter is positioned between the receiver and the motion trail of the detected target object, and the emission light received by the receiver passes through the emission filter;
the excitation light source is used for responding to an emission instruction and emitting excitation light;
the receiver is used for receiving the emitted light emitted by the detected target object and quantitatively analyzing the fluorescein in the detected target object according to the emitted light.
2. The apparatus according to claim 1, wherein the excitation light source emits excitation light in a direction perpendicular to the movement track of the target object; and/or the moving direction of the emitted light received by the receiver is perpendicular to the moving track of the detected target object.
3. The device according to claim 1 or 2, wherein the excitation light source and the receiver are disposed on the same side of the motion trajectory of the detected object, and the excitation light emitted by the excitation light source and the emission light received by the receiver are two parallel light beams; and/or the excitation light source and the receiver are arranged in parallel.
4. The device according to claim 1 or 2, wherein the excitation light filter and the emission filter are arranged in parallel.
5. The device according to claim 1 or 2, wherein the excitation light emitted by the excitation light source moves in a direction opposite to the direction of movement of the emission light received by the receiver.
6. The apparatus of claim 1 or 2, wherein the excitation light source is spaced from the receiver by a predetermined distance, and the predetermined distance is determined according to the fluorescence duration of the detected target.
7. The apparatus according to claim 1 or 2, wherein the moving direction of the moving track of the detected object is a direction from the excitation light source to the receiver.
8. A method for quantitative fluorescence analysis, which is applied to the quantitative fluorescence analysis device according to any one of claims 1 to 7, comprising:
responding to an emission instruction, controlling an excitation light source to emit excitation light, and irradiating the excitation light to a detected target object through an excitation light filter;
controlling the detected target object to move along the target movement direction at a preset speed;
if the detected target object moves to the position corresponding to the receiver, controlling the receiver to receive the emitted light which is emitted by the detected target object and passes through the emission filter;
and carrying out quantitative analysis on the fluorescein in the detected target object according to the emitted light received by the receiver.
9. The method of claim 8, wherein the target motion direction is a direction from the excitation light source to the receiver; and/or a preset distance is arranged between the excitation light source and the receiver, and the preset distance is determined according to the fluorescence duration of the detected target object.
10. The method of claim 8, wherein the quantitative analysis of fluorescein in the target object is performed based on the emitted light received by the receiver, comprising:
and determining the quantity of the fluorescein in the detected target object according to the preset speed, the preset distance between the excitation light source and the receiver and the light intensity of the emitted light.
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| CN108469525A (en) * | 2018-06-30 | 2018-08-31 | 武汉生之源生物科技股份有限公司 | A kind of fluoroimmunoassay device and method |
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