CN103555562A - Blood culture instrument - Google Patents
Blood culture instrument Download PDFInfo
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- CN103555562A CN103555562A CN201310531267.9A CN201310531267A CN103555562A CN 103555562 A CN103555562 A CN 103555562A CN 201310531267 A CN201310531267 A CN 201310531267A CN 103555562 A CN103555562 A CN 103555562A
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- Prior art keywords
- rocker
- connecting rod
- motor
- cultivation instrument
- blood cultivation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Abstract
The invention relates to a blood culture instrument comprising a fixing frame (13), a swinging frame (8), a motor (4) and a speed reducer (5), wherein the fixing frame (13) is arranged on a base (12); the swinging frame (8) is articulated in the fixing frame (13), and the motor (4) and the speed reducer (5) are fixedly arranged on the base (12); the swinging frame (8) is of a hollow rectangle structure, the swinging frame (8) is arranged in the fixing frame (13) in a swinging manner, a first connection rod (6) is articulated with the swinging frame (8) through a second connection rod (7) on the swinging frame (8) and is also articulated on the speed reducer (5); an optical detection sensor is arranged on the swinging frame (8) and swings along the swinging of the swinging frame (8). Therefore, according to the blood culture instrument disclosed by the invention, a situation of inaccurate detection caused by nonalignment in the movement of the motor and difference in positions of detection holes is avoided; in addition, each detector is mutually independent, and the position of each detector is found out without depending on the movement of the motor, so that the time required by the movement of the motor is saved, and the detection speed is increased.
Description
Technical field
The present invention relates to technical field of medical instruments, specifically relate to a kind of blood cultivation instrument.
Background technology
Automated blood culture instrument is comprised of constant-temperature incubation system and detection system.Traditional blood bacteria culture instrument adopts rotary type, the hemoculture instrument of rotary type makes nutrient solution in the culturing bottle state in constantly shaking up by the mode of rotating, on the rotation face of the hemoculture instrument of rotary type, there is cylindrical detection hole inside, diffusion by different level from inside to outside, every layer is detected hole and is uniformly distributed, every layer is equipped with a detecting position, by stepper-motor, is walked and is found one by one Blood culture bottle to be detected.
The Chinese utility model patent that the same Applicant of present patent application is ZL201220738836.8 in the patent No. of first to file discloses a kind of rotary type motion control device for fully automatic blood bacteria culture instrument, and it comprises rotating disk, stepper-motor, signal control processing unit, locating stop piece, start bit position transducer.Wherein, the culture apparatus that rotating disk is sample, is distributed with a plurality of sample positions of placing Blood culture bottle, locating stop piece is fixed in the start bit of disk edge, start bit position transducer is fixed under rotating disk, and in order to detection and location catch, rotating disk is connected with stepper-motor.Stepper-motor is all connected by data line with signal control processing unit with start bit position transducer.This kind of motion control device has comparatively accurate positioning function, can automatically move according to control signal, realizes rotary cultivation.But, nutrient solution in this kind of revolving sample flashing lightning magnetic field detector rotates along with rotating the rotation of face, the speed that nutrient solution in culturing bottle shakes up is slower, and stepper-motor is found bottle to be detected by walking, and for detecting, the accuracy of hole site and the accuracy requirement of motor are higher.In addition, detect the deviation that walking of gap small between hole site or motor all can cause detecting position and detect contraposition between hole, thereby cause detecting the huge change of data, affect detected result.
Summary of the invention
For overcoming above-mentioned shortcomings and deficiencies of the prior art, the invention provides a kind of shuttle-type blood cultivation instrument, proofing unit in this blood cultivation instrument swings along with the swing of rocker, avoid motor walk inaccurate and detect the inaccurate phenomenon of detection that hole site difference is brought, and each proofing unit is separate, do not need to walk to find position by motor, saved motor and walked the needed time, accelerated detection speed.
For achieving the above object, the present invention is achieved through the following technical solutions: a kind of blood cultivation instrument, comprises the anchor being arranged on base; Be hinged on the rocker in anchor; Be fixedly mounted on motor, step-down gear on base, described rocker is hollow type rectangular configuration and swingable be arranged in described anchor and hinged with the first connecting rod being hinged on described step-down gear by the second connecting rod on it.
Further preferably, described step-down gear is hinged by one end of rotating disk mounted thereto and first connecting rod, and the other end and the second connecting rod on rocker of described first connecting rod are hinged;
Further preferably, rotating being arranged on the rotating disk that departs from described center of turntable position of described first connecting rod.
In above-mentioned arbitrary scheme, preferably, in the side of described anchor, offer successively from top to down spacing hole.
In above-mentioned arbitrary scheme, preferably, the both sides of described rocker are installed with cylindrical boss.
In above-mentioned arbitrary scheme, preferably, described rocker is arranged in described spacing hole by the cylindrical boss on it is rotating.
In above-mentioned arbitrary scheme, preferably, the quantity of described spacing hole equates with the quantity of described cylindrical boss.
In above-mentioned arbitrary scheme, preferably, described rocker is hierachical structure, and every layer of rocker is mutually permanently connected by connecting rod.
In above-mentioned arbitrary scheme, preferably, on the base of described connecting rod lower end, displacement sensor is housed.Described position transducer detects the displacement of described rocker.
In above-mentioned arbitrary scheme, preferably, the end of described connecting rod is fixedly connected with position catch, and position catch has avoided causing due to the drive of motor the mistake load of rocker effectively.
Preferably, on described base, be also installed with controlling box, in described controlling box, signal control device be installed.
In above-mentioned arbitrary scheme, preferably, described motor is direct-current machine.
In above-mentioned arbitrary scheme, preferably, described motor is alternating current machine.
In above-mentioned arbitrary scheme, preferably, described rocker is at least divided into one deck, and every layer is at least provided with the location of listing and indexing.
In above-mentioned arbitrary scheme, preferably, on the described location of often listing and indexing, be at least distributed with a detecting position.
In above-mentioned arbitrary scheme, preferably, on described detecting position, optical detecting sensor is installed.
Preferably, described optical detecting sensor comprises light emitting diode array, spectral filter, photorectifier, interior hollow round column body base, light emitting diode constant current power supply, described light emitting diode matrix is comprised of a plurality of LED, described spectral filter is positioned at hollow round column body center near apical position, a little less than top; Described photorectifier is positioned at the vertical lower of spectral filter, the sensitive surface of described photorectifier towards spectral filter and with spectral filter plane parallel.
Preferably, the number of described a plurality of LED is 4.
Preferably, described a plurality of LED are evenly embedded in interior hollow round column body base top surrounding, and position is a little less than the top planes of right cylinder base, and become 45 degree overturning angles with top planes.
Preferably, described spectral filter plane is vertical with right cylinder axis, and is fixed by thread complete set.
Preferably, the output line of described photorectifier connects highly sensitive lower noise linear amplifier circuit.
Preferably, described light emitting diode array is connected in series and is connected to light emitting diode constant current power supply, to guarantee the stability of LED brightness.
Preferably, described light emitting diode selects the highlighted LED of same model, same parameter, emission center wavelength 630nm as excitation light source.
Preferably, described spectral filter is vacuum plating spectral filter or red opticglass.
Preferably, the centre wavelength of described red opticglass is 630nm, and bandwidth 10nm-20nm, with the interference of effective filtering veiling glare.
Preferably, described photorectifier is arranged on photorectifier seat, and photorectifier seat is placed on and detects in base.
Further preferably, described detection base is by being screwed on interior hollow round column body base, and described spectral filter is placed on and detects on base, by detector tube lid, is fixed on and is detected on base.
Compared with prior art the invention has the advantages that: the proofing unit in this blood cultivation instrument swings up and down to mix the nutrient solution in Blood culture bottle by rocker, on the rocker of shuttle-type hemoculture instrument, be uniformly distributed cylindrical detection hole, each detects after hole and fixes a detecting position corresponding with it.Rocker to swing up and down amplitude large, the speed that shakes up is accelerated, after each detection hole, fix a proofing unit, proofing unit swings along with the swing of rocker, avoided the motor phenomenon inaccurate and that detect the detection misalignment that hole site difference brings that walks to occur, and each proofing unit is separate, does not need to walk to find position by motor, saved the time that motor walks, accelerated detection speed, improved detection efficiency.
Accompanying drawing explanation
Fig. 1 is the perspective view of optical detection apparatus one preferred embodiment of the blood cultivation instrument in the present invention;
Fig. 2 is the optical detection apparatus of blood cultivation instrument of the present invention main TV structure schematic diagram embodiment illustrated in fig. 1;
Fig. 3 is the optical detection apparatus of blood cultivation instrument of the present invention plan structure schematic diagram embodiment illustrated in fig. 1;
Fig. 4 is the optical detection apparatus of blood cultivation instrument of the present invention left TV structure schematic diagram embodiment illustrated in fig. 2;
Fig. 5 is the three-dimensional arrangement view of optical detecting sensor one preferred embodiment in embodiment illustrated in fig. 3 in the optical detection apparatus of blood cultivation instrument of the present invention;
Fig. 6 is the sectional structure schematic diagram of the optical detecting sensor shown in Fig. 5 in the optical detection apparatus of blood cultivation instrument of the present invention;
Fig. 7 is the internal frame diagram of the signal control processing unit in the optical detection apparatus of blood cultivation instrument of the present invention;
Fig. 8 is the optical detection apparatus of blood cultivation instrument of the present invention detection journey schema embodiment illustrated in fig. 1.
Embodiment
Below in conjunction with drawings and Examples, a preferred embodiment of the present invention is further elaborated to explanation.
As Figure 1-5, a kind of optical detection apparatus of blood cultivation instrument, comprises the anchor 13 being arranged on base 12; Be hinged on the rocker 8 in anchor 13; Described rocker 8 can swing up and down in described anchor 13.The motor 4 being fixedly mounted on base 12 is coaxially connected with step-down gear 5.The Sports band movable pendulum frame 8 of described motor 4 swings up and down the mixing of the nutrient solution in the culturing bottle 10 of realizing on rocker 13 in described rocker 13.Described rocker 8 is hollow type rectangular configuration, and rocker 8 is swingable is arranged in described anchor 13 and hinged with the first connecting rod 6 being hinged on described step-down gear 5 by the second connecting rod 7 on it.The swing mode of rocker 8 realizes in the following way: described motor 4 drives described step-down gear 5 motions, on the output shaft of described step-down gear 5, permanent sleeve is connected to rotating disk 14, described step-down gear 5 is hinged with one end of first connecting rod 6 by rotating disk 14, and the second connecting rod 7 on the other end of described first connecting rod 6 and rocker 8 is hinged; Junction between first connecting rod 6 and described rotating disk 14 can rotate, and rotation can be realized equally in the hinged place between first connecting rod 6 and second connecting rod 7; Second connecting rod 7 is fixedly connected with described rocker.First connecting rod 6 is rotating to be arranged on the rotating disk 14 that departs from described rotating disk 14 central positions, when the motion of motor 4 drives described step-down gear 5 motions, then drives the motion of described rotating disk 14 motions, described rotating disk 14 to drive described rocker to move reciprocatingly.
Side at described anchor 13 offers spacing hole 15 from top to down successively; The both sides of described rocker 8 are installed with cylindrical boss 16.Rocker 8 is arranged in spacing hole 15 by the cylindrical boss 16 on it is rotating, and the quantity of spacing hole 15 equates with the quantity of described cylindrical boss 16.When driven by motor first connecting rod 6, second connecting rod 7 motion, rocker 8 swings up and down along with the swing of the two.Rocker 8 is hierachical structure, and every layer of rocker 8 is mutually permanently connected by connecting rod 17, and rocker 8 is at least divided into one deck, and every layer is at least provided with the location 1 of listing and indexing, is at least distributed with a check point on the location 1 of often listing and indexing.In the present embodiment, described rocker 8 is six layers of structure, and every one deck is provided with two locations of listing and indexing, and the location of often listing and indexing is provided with ten check points.On described detecting position 1, optical detecting sensor is installed.The end of described connecting rod 17 is fixedly connected with position catch 2, and the arranging of position catch 2 effectively avoided due to the overload of rocker 8 or the damage of crossing the mechanical means that motion causes.
As shown in Fig. 3-4 and Fig. 8, on the base of connecting rod 17 lower ends, displacement sensor 20 is housed, described displacement sensor 20 detects the displacement of described rocker 8 and converts electric signal transmission to being arranged on the signal control device in the controlling box 18 on base 12.In the present embodiment, described controlling box 18 is provided with array fan on interior.Signal control processing unit in described signal control device comprises clock 501, timer 502, controller 503, arithmetical unit 504, storer 505, motor-drive circuit 506, LED drive circuit 507, pre-amplification circuit 508, multiplex electronics 509.Clock 501 provides basis of time for whole detection system, in timer 502, sets detection time, and controller is controlled the motion of 503 motors, LED bright, go out etc.Arithmetical unit 504 carries out logic, arithmetical operation.For example, motor movement arrives step number of concrete sample position etc., and the result feedback of computing, to controller 503, is carried out to relating operation by controller 503.Storer 505 is preserved the relevant information of culturing bottle 10 interior samples and the intermediate operations result of internalarithmetic and net result etc.Motor-drive circuit 506 control motors motion, stop and speed etc.LED drive circuit 507 is controlled the light on and off of LED.In view of general photorectifier 403 is when changing into current signal by optical signal, the current signal changing into is generally all more weak, so the signal of photorectifier 403 is input to after pre-amplification circuit 508 amplifies in advance again and carries out computing in arithmetical unit, after each detection hole, be fixed with corresponding detecting position, so many signal can not be exported and gather to controller, so all will increase multiplex electronics 509 between controller and LED drive circuit and data acquisition circuit.
As shown in Fig. 5-6, optical detecting sensor comprises: light emitting diode array 401, spectral filter 402, photorectifier 403, interior hollow round column body base 404, light emitting diode constant current power supply 405.Light emitting diode matrix 401 is composed in series by 3-8 LED, is preferably 4, is evenly embedded in the top surrounding of interior hollow round column body base 404, and position is a little less than the top planes of interior hollow round column body base 404, and becomes 45 degree overturning angles with top planes.Photorectifier 403 is arranged on photorectifier seat 406, photorectifier seat 406 is placed on and detects in base 407, detect base 407 by being screwed on interior hollow round column body base 404, spectral filter 402 is placed on and detects on base 407, by detector tube lid 408, is fixed on and is detected on base 407.Photorectifier 403 is fixed on the bottom of spectral filter 402, sensitive surface towards spectral filter 402 and with spectral filter 402 plane parallel, photorectifier output line 409 connects highly sensitive lower noise linear amplifier circuit.Described 4 light emitting diodes are connected in series and are connected to the constant current source power supply circuit of LED, to guarantee the stability of LED brightness.4 light emitting diodes are selected the highlighted LED that same model, same parameter, emission center wavelength are 630nm, as excitation light source.Described spectral filter 402 is red opticglass), centre wavelength is 630nm, bandwidth 10nm, narrow bandwidth is the interference of filtering veiling glare effectively.At the bottom of light emitting diode illuminates special culturing bottle with 45 degree angles, for the voltage sensor indicator in special culturing bottle polymeric membrane provides excitation light source, adopt 4 just in order to have enough brightness and brightness even, simultaneously also in order to guarantee that photorectifier is in linear work district.Photodiode constant current power supply guarantees stable luminescence.
Fig. 8 is the detection journey schema in the optical detection apparatus of blood cultivation instrument, after bringing into operation, can put into sample, the relevant information of input sample, name such as this sample patient, sex, at age etc., waits the detection time that runs to default, such as it is 10 minutes, within every 10 minutes, detect once, motor 4 reduces speed now, until position catch 2 blocks displacement sensor 20.Such as, displacement sensor 20 is a kind of photo-sensors, position catch 2 blocks the optical signal of displacement sensor 20, exports from lower level and becomes high level, and this high level signal is input to controller, motor stop motion, start to detect, LED lamp is lighted in order successively, and illumination is mapped to the bottom of sample culturing bottle 10, through culturing bottle 10, reflex to spectral filter 402, and then to photorectifier 403.Reflected light is different according to the color of bottle bottom, the light intensity being received by photorectifier 403 is also just different, photorectifier 403 is transformed into current signal optical signal, through pre-amplification circuit 508, change into voltage signal, be transferred to arithmetical unit 504, arithmetical unit, through arithmetical operation, is depicted as the growth curve of bacterium and reports to the police.For example the mode of this arithmetical operation is to calculate the slope of the nearest growth curve of bacteria detecting for 30 times of some samples, and judges positive and negative with this.If after testing the 40th time now, the voltage signal detecting is V (40), and the detectable voltage signals of the 10th time is V (10), and the slope of so nearest 30 bacterial detection growth curves is K=V (40)-V (10)/30, for another example, when this slope is greater than 1.5, just can define bacterial growth, so as K > 1.5, this sample is reported to the police positive, when K≤1.5, enter normal operating condition, wait for next time and detecting.If this Samples detection number of times reaches considerable number of times, such as this number of times is 800 times, all do not report to the police positive, according to microorganism growth characteristic, can judge in this sample there is no bacterium, this sample is by the feminine gender of reporting to the police.In the present embodiment, to be minicomputer etc. have the machinery equipment of controlling function with described controller.
Above combination specific embodiments of the invention are described in detail, but be not limitation of the present invention, every foundation technical spirit of the present invention all belongs to technical scope of the present invention to any simple modification made for any of the above embodiments, also it should be noted that: in embodiment not all matters, big and small list one by one associated components, but do not affect the understanding of those skilled in the art to related art scheme.According to the category of technical scheme of the present invention, comprise the arbitrary combination between each part mentioned above.
Claims (10)
1. a blood cultivation instrument, comprises the anchor (13) being arranged on base (12); Be hinged on the rocker (8) in anchor (13); Be fixedly mounted on motor (4), step-down gear (5) on base (12), it is characterized in that: described rocker (8) is hollow type rectangular configuration, being arranged in described anchor (13) that rocker (8) is swingable is also hinged with the first connecting rod (6) being hinged on described step-down gear (5) by the second connecting rod on it (7).
2. blood cultivation instrument as claimed in claim 1, it is characterized in that: step-down gear (5) is hinged with one end of first connecting rod (6) by rotating disk (14) mounted thereto, and the second connecting rod (7) on the other end of described first connecting rod (6) and rocker (8) is hinged.
3. blood cultivation instrument as claimed in claim 1 or 2, is characterized in that: described first connecting rod (6) is rotating to be arranged on the rotating disk (14) that departs from described rotating disk (14) central position.
4. blood cultivation instrument as claimed in claim 1, is characterized in that: the side at described anchor (13) offers spacing hole (15) from top to down successively.
5. blood cultivation instrument as claimed in claim 1, is characterized in that: the both sides of described rocker (8) are installed with cylindrical boss (16).
6. blood cultivation instrument as claimed in claim 5, is characterized in that: described rocker (8) is arranged in spacing hole (15) by the cylindrical boss on it (16) is rotating.
7. blood cultivation instrument as claimed in claim 6, is characterized in that: the quantity of described spacing hole (15) equates with the quantity of described cylindrical boss (16).
8. the blood cultivation instrument as described in claim 1 or 6, is characterized in that: described rocker (8) is hierachical structure, and every layer of rocker (8) is mutually permanently connected by connecting rod (17).
9. blood cultivation instrument as claimed in claim 8, is characterized in that: on the base of described connecting rod lower end, displacement sensor is housed.
10. blood cultivation instrument as claimed in claim 8, is characterized in that: the end of described connecting rod (17) is fixedly connected with position catch (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310531267.9A CN103555562A (en) | 2013-11-01 | 2013-11-01 | Blood culture instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310531267.9A CN103555562A (en) | 2013-11-01 | 2013-11-01 | Blood culture instrument |
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| CN103555562A true CN103555562A (en) | 2014-02-05 |
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| CN201310531267.9A Pending CN103555562A (en) | 2013-11-01 | 2013-11-01 | Blood culture instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881904A (en) * | 2014-03-28 | 2014-06-25 | 山东鑫科生物科技股份有限公司 | Oscillating movement control device and control method |
| CN105039151A (en) * | 2015-06-25 | 2015-11-11 | 山东鑫科生物科技股份有限公司 | Blood culture system |
| CN106318850A (en) * | 2016-11-07 | 2017-01-11 | 曲雪红 | Blood bacterial culture device |
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| CN103063639A (en) * | 2012-12-31 | 2013-04-24 | 山东鑫科生物科技股份有限公司 | Microbial growth optical detection sensor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881904A (en) * | 2014-03-28 | 2014-06-25 | 山东鑫科生物科技股份有限公司 | Oscillating movement control device and control method |
| CN105039151A (en) * | 2015-06-25 | 2015-11-11 | 山东鑫科生物科技股份有限公司 | Blood culture system |
| CN105039151B (en) * | 2015-06-25 | 2017-08-01 | 山东鑫科生物科技股份有限公司 | A kind of Blood culture machine |
| CN106318850A (en) * | 2016-11-07 | 2017-01-11 | 曲雪红 | Blood bacterial culture device |
| CN106318850B (en) * | 2016-11-07 | 2018-09-28 | 青岛市妇女儿童医院 | A kind of blood germ culturing device |
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Application publication date: 20140205 |