CN206411011U - A kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing - Google Patents
A kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing Download PDFInfo
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- CN206411011U CN206411011U CN201720116412.0U CN201720116412U CN206411011U CN 206411011 U CN206411011 U CN 206411011U CN 201720116412 U CN201720116412 U CN 201720116412U CN 206411011 U CN206411011 U CN 206411011U
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- chlorophyll fluorescence
- optical fiber
- sample
- module
- stepper motor
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Abstract
The utility model provides a kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing, mainly includes：Exciting light generation module, chlorophyll fluorescence detection module, MCU module, touch screen type display terminal and USB interface, loop sample placed cavity, stepper motor, optical fiber etc..The system can be automatically to the chlorophyll fluorescences of multiple samples number of repetition and interval time automatic measurement according to setting, overcome existing chlorophyll fluorescence instrument passage few, changing sample every time all needs the shortcoming of manual intervention, more data volumes are provided for research plant physiology and environmental factor etc., substantial amounts of manpower and time are saved simultaneously, difficulty of the night to multiple sample duplicate measurements is especially reduction of.
Description
Technical field
The utility model is related to chlorophyll fluorescence detection technique field, especially designs a set of multiple with Multi-example timesharing
The chlorophyll fluorescence test system of automatic measurement function.
Background technology
Chlorophyll fluorescence signal can reflect photosynthesis of plant mechanism and its protected from environmental, be that a kind of research is planted
The nondestructiving detecting means of thing photosynthesis and environmental concerns, chlorophyll fluorescence techniques are in photosynthesis, plant stress physiology, water
The multiple fields such as raw biology, oceanography are widely used.At present, the price of chlorophyll fluorescence instrument is somewhat expensive, mostly
Only one of which passage, because the test of many chlorophyll fluorescences needs to carry out dark adaptation to leaf, this causes one sample of each pair to enter
Manual intervention is all needed during row manual measurement, causing the measurement to multiple samples becomes extremely difficult, the especially test at night.
Utility model content
In view of the above-mentioned problems existing in the prior art, there is the multiple automatic measurement of Multi-example timesharing this application provides one kind
The chlorophyll fluorescence test system of function.The system can be automatically secondary according to the repetition of setting to the chlorophyll fluorescence of multiple samples
Number and interval time automatic measurement, it is few to overcome existing chlorophyll fluorescence instrument passage, and sample is changed every time all needs manual intervention
Shortcoming, be research plant physiology and environmental factor etc. provide more data volumes, while save substantial amounts of manpower and
Time, especially it is reduction of difficulty of the night to multiple sample duplicate measurements.
The technical solution of the utility model is as follows：
A kind of chlorophyll fluorescence tester, including control section, detection part and sample stage；
The control section includes display and control terminal (1) and the microprocessor i.e. MCU module (2) being attached thereto；Institute
Stating detection part includes chlorophyll fluorescence detection module (3) and exciting light generation module (4)；The chlorophyll fluorescence detects mould
Block (3) and exciting light generation module (4) are connected with MCU module (2) respectively；
The sample stage includes bearing (5) and stepper motor (6), and the stepper motor (6) is connected with MCU module (2)；Institute
State the rotor connection sample disc (7) of stepper motor (6)；
The circumference of the sample disc (7) is the hollow annular chamber of a circle, and the outer wall of the annular chamber can be opened；The ring
Annular chamber is isolated into the loop sample placed cavity (S3) of segmentation perpendicular to the dividing plate (S4) of outer wall by the setting of shape intracavitary；
The side of the loop sample placed cavity (S3) of each section of isolation is mounted on optical fiber (S2), and the optical fiber (S2) is in step
The drive backspin of stepper motor is given to fixed angle, is alignd with optical fiber (S1), reaches and chlorophyll fluorescence detection module (3) and excites
The purpose of light generation module (4) connection, by stepper motor multiple rotary, multiple optical fiber (S2) on annular chamber respectively with optical fiber
(S1) align.
It is preferred that, display and control terminal (1) they are touch screen type display terminal, are provided with USB interface.
It is preferred that, loop sample placed cavity (S3) bottom can selectively set curved tube passage, reach ventilation resistance
The purpose of light.
It is preferred that, it is also an option that property sets LED in the loop sample placed cavity (S3).
The utility model is beneficial to be had technical effect that：
The fluorescence instrument system can complete OJIP the and PAM pulse chlorophyll fluorescence single channels of traditional chlorophyll fluorescence instrument
Function is measured, is had further the advantage that：
1st, multiple samples can automatically be measured, when switching between two test samples, without manual intervention, and
It is using MCU module control stepper motor automatic rotary conversion sample.Substantial amounts of labour has been saved, night test has especially been solved tired
Difficult the problem of.
2nd, test experiments data system is named automatically, convenient management.
3rd, multiple sample timesharing are tested, the manufacturing cost of instrument has been saved, while being easy to the sample that can once test
The expansion of quantity.
4th, sample under MCU coordination can automatic shift, realize to multiple sample automatic measurements.
5th, after measurement terminates every time, automatic it can start to hold according to project name, sample number into spectrum, experiment number numbering, experiment
The combination name file of capable time is simultaneously preserved.
6th, division board can be placed between sample to eliminate when a sample is in test, light can shine on other samples and
Influence is produced on test sample.
7th, LED is can configure in annular chamber, light adaptation condition is provided for sample.
The utility model and the contrast situation of several prior arts are as follows：
Patent CN201220018939 each passage is using an independent probe, because optical sensor compares
Costliness, such total price is higher.
What patent CN201220134401 was surveyed is not and the whole test platform with life and plant in irregular shape
In a simple circular annular seal space, this implements fairly simple, but inconvenient operation, because operator can not
It can stay in annular seal space, while plant can die in annular seal space.What is tested in the design is with life and in irregular shape
Plant, and test platform is divided into cabin and two parts out of my cabin, and this is accomplished by two parts and cleverly designs and coordinate so that swash
It is luminous to travel to intracavitary, while ambient light does not pollute chlorophyll fluorescence again.Patent CN201220134401 circular opticals are put down
Platform can not replace the ring cavity structure in the design.
Patent CN201521141187 table designs are fairly simple, it is not necessary to consider the influence of ambient light.As described above,
Circular optical platform can not replace the ring cavity structure in the design.
Chlorophyll fluorescence is required of excitation source when testing can not pollute other test samples, and ambient light, which can not pollute, appoints
What sample, for the ease of operating and saving optics, excitation source cannot be simply rested in a circular cavity,
But patent CN201610355496 rotation platforms do not consider these factors, therefore it can not also replace the design in the present invention.
As it was previously stated, patent CN201521141187 circular optical platform can not equally replace the annular chamber in the design
Structure.
Brief description of the drawings
Fig. 1 is the system construction drawing of the utility model one embodiment；
Fig. 2 shows floor map for the annular chamber of the utility model one embodiment；
Fig. 3 is the workflow diagram of the utility model one embodiment.
Embodiment
With reference to specific embodiments and the drawings, the utility model is described in further detail, embodiment of the present utility model
Including but not limited to example below, therefore the utility model protection domain should not be limited with this embodiment content.
As shown in Figure 1, the system includes control section, detection part and sample stage；
The control section includes display and control terminal 1 and the microprocessor i.e. MCU module 2 being attached thereto；The inspection
Surveying part includes chlorophyll fluorescence detection module 3 and exciting light generation module 4；The chlorophyll fluorescence detection module 3 and
Exciting light generation module 4 is connected with MCU module 2 respectively；
The sample stage includes bearing 5 and stepper motor 6, and the stepper motor 6 is connected with MCU module 2；The stepping electricity
The rotor connection sample disc 7 of machine 6；
The circumference of the sample disc 7 is the hollow annular chamber of a circle, and the outer wall of the annular chamber can be opened；The annular
Intracavitary sets the loop sample placed cavity S3 that annular chamber is isolated into segmentation perpendicular to the dividing plate S4 of outer wall；
The loop sample placed cavity S3 of each section of isolation side is mounted on optical fiber S2 (Fig. 1 is stereogram), the light
Fine S2 is given to fixed angle in the drive backspin of stepper motor, is alignd with optical fiber S1, reach with chlorophyll fluorescence detection module 3 and
The purpose that exciting light generation module 4 is connected, by stepper motor multiple rotary, multiple optical fiber S2 on annular chamber respectively with optical fiber
S1 aligns.
In one preferred scheme, display and control terminal 1 are touch screen type display terminal, are provided with USB interface.
In one preferred scheme, the loop sample placed cavity S3 bottoms can selectively set curved tube passage, reach
The purpose for light blocking of ventilating.
In one preferred scheme, it is also an option that property sets LED in the loop sample placed cavity S3.
The model selection and effect of each main modular are as follows：
1st, display and control terminal 1 select touch screen type display terminal, are provided with USB interface, and effect is data and ginseng
Number input and output.
2nd, MCU module 2：Receive the parameter setting from display and control terminal 1, the generation for coordinating exciting light and chlorophyll
The sampling of fluorescence, while Driving Stepping Motor 6 rotates the angle specified, preserves the functions such as file.
3rd, chlorophyll fluorescence detection module 3：For testing chlorophyll fluorescence, optical filter is used to select 700nm extremely
750nm photon arrives at photodiode, and the circuit of photodiode converts amplifying circuit and filtering, voltage by Current Voltage
Amplifying circuit, is then connected with MCU AD conversion units.
4th, exciting light generation module 4：Blue light or wavelength for producing excitation Photosystem II (PSII) are less than
680nm feux rouges and excitation Photosystem I (PSI) far-red light (wavelength is higher than 750nm), optical filter is used for light wave
Long limitation.The light source of excitation PSII systems has three, is able to provide high-frequency impulse, saturation pulse and actinic light.MCU moulds
The PWM module of block 2 is connected with power amplification circuit module, and then connects LED, then controls LED intensity.
Exciting light generation module 4, chlorophyll fluorescence detection module 3 are connected with the optical fiber S1 of a Y-shaped, optical fiber S1's
The other end aligns with the optical fiber S2 on the annular chamber under the driving of stepper motor just in test.
5th, loop sample placed cavity S3：The samples such as leaf can be placed in annular chamber, on annular chamber, be mounted with light
Fine S2, each optical fiber S2 is narrowly focused towards a measurand.Loop sample placed cavity S3 can stepper motor 6 drive backspin
Turn the angle of setting so that optical fiber S2 is just with being connected the optical fiber of chlorophyll fluorescence detection module 3 on loop sample placed cavity S3
S1 is aligned.
6th, stepper motor 6：Stepper motor 6 is connected with MCU module 2, and the angle specified is rotated under the control of MCU module 2,
So that the optical fiber S2 alignd on loop sample placed cavity S3 with measurand is just with being connected to chlorophyll fluorescence detection module 3
The optical fiber S1 other ends align.
The application method of the present apparatus is as follows：
During test, sample dispersion in loop sample placed cavity S3, such as the angle between them is decile (angle
What decile was not required, the angle between two samples can be any, need to only be set in program, program can be according to given
Angle controls the rotation of stepper motor 6).
Angled scale on loop sample placed cavity S3, such as every 15 degree of scales, so has on 360 degree of circumference
One optical fiber S2 hole can be installed, so round can place 24 simultaneously in one week on 24 scales, the position of every 15 degree of scales
24 samples automatically can be switched over test, of course, it is possible to which more samples can once be accommodated by extending by individual optical fiber.
Can be with the support and clip of riding position size adjustable in loop sample placed cavity S3 so that sample and optical fiber pair
Together, the S2 (circumferentially thering is the S2 identified on multiple optical fiber, Fig. 1 to be one of them) for example identified on Fig. 1.
By display and control terminal 1, the angle between project name, sample size, each sample is set, if not
Decile, the angular interval between them is inputted can one by one, select the species of test, such as FoFm tests, OJIP is tested,
PAM chlorophyll fluorescences (ChlF) test etc. and other parameters, these parameters are ripe in the literature, not in the utility model
Claim among, be not described in detail one by one herein.Need to set the stand-by period between each sample simultaneously, and from first
To last sample test it is complete after, if need retest, and retest number of times.
The lower testing button of point, stepper motor 6 drives ring rotation, and MCU module 2 can send order, tell stepper motor 6 to revolve
The angle turned so that the optical fiber S2 on loop sample placed cavity S3 aligns with the optical fiber S1 being connected on exciting light generation module 3,
Then accordingly tested, and the automatic time for starting to perform according to project name, sample number into spectrum, experiment number numbering, experiment
Combination life file and preserve.
Preferably, MCU module 2 can include with data transmission network：WIFI network, 2G networks, 3G network, 4G networks,
LTE network is connected with blueteeth network, and test result can remotely pass to client, and client can check in the terminals such as mobile phone
As a result and modification experiment parameter.
The utility model is measured using automatic test sample of changing, can be in the case of unattended to chlorophyll
Fluorescence release object carries out continuously, periodically and automatically duplicate measurements, more effectively provides the dynamic of fluorescence associated parameter
Situation of change.
Claims (4)
1. a kind of chlorophyll fluorescence tester, including control section, detection part and sample stage；It is characterized in that：
The control section includes display and control terminal (1) and the microprocessor i.e. MCU module (2) being attached thereto；The inspection
Surveying part includes chlorophyll fluorescence detection module (3) and exciting light generation module (4)；The chlorophyll fluorescence detection module
And exciting light generation module (4) is connected with MCU module (2) respectively (3)；
The sample stage includes bearing (5) and stepper motor (6), and the stepper motor (6) is connected with MCU module (2)；The step
The rotor connection sample disc (7) of stepper motor (6)；
The circumference of the sample disc (7) is the hollow annular chamber of a circle, and the outer wall of the annular chamber can be opened；The annular chamber
The interior loop sample placed cavity (S3) for setting the dividing plate (S4) perpendicular to outer wall that annular chamber is isolated into segmentation；
The side of the loop sample placed cavity (S3) of each section of isolation is mounted on optical fiber (S2), and the optical fiber (S2) is in stepping electricity
The drive backspin of machine is given to fixed angle, is alignd with optical fiber (S1), reaches and chlorophyll fluorescence detection module (3) and exciting light production
The purpose of raw module (4) connection, by stepper motor multiple rotary, multiple optical fiber (S2) on annular chamber respectively with optical fiber (S1)
Alignment.
2. chlorophyll fluorescence tester according to claim 1, it is characterised in that display and control terminal (1) are touch screen type
Display terminal, is provided with USB interface.
3. chlorophyll fluorescence tester according to claim 1, it is characterised in that loop sample placed cavity (S3) bottom
Portion can selectively set curved tube passage, reach the purpose of ventilation light blocking.
4. chlorophyll fluorescence tester according to claim 1, it is characterised in that in the loop sample placed cavity (S3)
It is also an option that property sets LED.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720116412.0U CN206411011U (en) | 2017-02-08 | 2017-02-08 | A kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720116412.0U CN206411011U (en) | 2017-02-08 | 2017-02-08 | A kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing |
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| Publication Number | Publication Date |
|---|---|
| CN206411011U true CN206411011U (en) | 2017-08-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720116412.0U Expired - Fee Related CN206411011U (en) | 2017-02-08 | 2017-02-08 | A kind of chlorophyll fluorescence test system with the multiple automatic measurement function of Multi-example timesharing |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109490259A (en) * | 2017-09-11 | 2019-03-19 | 台达电子国际（新加坡）私人有限公司 | Multicolor fluorescence excitation and detection device and its foranalysis of nucleic acids equipment application |
| CN113075028A (en) * | 2021-04-30 | 2021-07-06 | 海宁凯兴新材料股份有限公司 | Wear resistance detection equipment for warp knitting cloth |
| CN113092428A (en) * | 2021-04-02 | 2021-07-09 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
-
2017
- 2017-02-08 CN CN201720116412.0U patent/CN206411011U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109490259A (en) * | 2017-09-11 | 2019-03-19 | 台达电子国际（新加坡）私人有限公司 | Multicolor fluorescence excitation and detection device and its foranalysis of nucleic acids equipment application |
| CN113092428A (en) * | 2021-04-02 | 2021-07-09 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
| CN113075028A (en) * | 2021-04-30 | 2021-07-06 | 海宁凯兴新材料股份有限公司 | Wear resistance detection equipment for warp knitting cloth |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170815 Termination date: 20200208 |