CN104198450B - Light path detection device for full-automatic time resolution detector - Google Patents
Light path detection device for full-automatic time resolution detector Download PDFInfo
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- CN104198450B CN104198450B CN201410432610.9A CN201410432610A CN104198450B CN 104198450 B CN104198450 B CN 104198450B CN 201410432610 A CN201410432610 A CN 201410432610A CN 104198450 B CN104198450 B CN 104198450B
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Abstract
The invention discloses a light path detection device for a full-automatic time resolution detector. The light path detection device comprises a control module, a light source component, a photomultiplier component, a Y-shaped optical fiber, a photocell feedback module, a fluorescence excitation interface, a detection interface and a dark room structure, wherein the fluorescence excitation interface and the detection interface are arranged on the dark room structure in a separation manner and are arranged vertically; the photomultiplier component is connected with the detection interface; a single end of the Y-shaped optical fiber is connected with the light source component and is used for splitting a light beam into two halves; one of the two ends of the Y-shaped optical fiber is connected with the fluorescence excitation interface; the other one of the two ends of the Y-shaped optical fiber is connected with the photocell feedback module; the control module is respectively connected with the light source component, the photomultiplier component and the photocell feedback module; the Y-shaped optical fiber is an quartz optical fiber. The light path detection device for the full-automatic time resolution detector is smart in design and simple in structure, can achieve full automation, is low in cost, good in detection repeatability and high in accuracy, and is applicable to on-scale popularization and application.
Description
Technical field
The present invention relates to a kind of full-automatic time resolution detector, more particularly to a kind of to detect for full-automatic time resolution
The light path detecting device of instrument.
Background technology
Time-resolved fluorescence method is with trivalent rare earth ionses (lanthanide series：Europium, bismuth, samarium etc.) and its sequestration thing be tracer
Come labelled antigen, antibody, nucleic probe or biologically active cell, after trivalent rare earth ionses and antigen, antibody complexation, through strengthening
The potentiation of agent is processed, and produces the strong fluorescence of long half time, fluorescence, so as to substantially increase the sensitivity of detection, accuracy, this
Method has become a most promising analysis means in modern biomedical research and clinical ultramicron inspection, is mesh
One of the top technology of front ultramicroanalysis and method.
Light path detecting device is one of key device in Time-resolved fluorescence assay system, mainly by single photon counting
Method measures the numerical value of fluorescence intensity.The at present light path detecting device complex structure used by Time-resolved fluorescence assay instrument, light path
Short, exciting light and launching light easily make detection signal produce interference in same optical axis, realize that full automatic difficulty, than larger, increases
Personnel cost is added, easily also produce interference between reaction utensil while having detected, for the inspection knot such as the repeatability of test, accuracy
Fruit produces impact.
The content of the invention
It is an object of the invention to provide a kind of light path detecting device for full-automatic time resolution detector, which has
The high spy of simple structure, achievable full-automatic continuous detecting, good ductility, low cost, the reproducible and accuracy in detection of detection
Point.With the problems referred to above that the light path detecting device for solving full-automatic time resolution detector in prior art is present.
It is that, up to this purpose, the present invention is employed the following technical solutions：
A kind of light path detecting device for full-automatic time resolution detector, which includes control module, light source assembly, light
Electricity multiplication tube assembly, y-type optical fiber, light cell feedback module, fluorescence excitation interface, detection interface and darkroom structure, wherein, it is described
Fluorescence excitation interface and detection interface are separately positioned in the darkroom structure, and both are arranged vertically, the photomultiplier tube
Component is connected with the detection interface, and light beam is divided into two by the single-ended connection light source assembly of the y-type optical fiber, and the one of its both-end
End connection fluorescence excitation interface, other end connection light cell feedback module, the control module are electrically connected light source assembly, light
Electricity multiplication tube assembly and light cell feedback module, and the y-type optical fiber is silica fibre.
Especially, the light source assembly includes xenon flash lamp, saturating ultraviolet light collecting lenss group and blue light bandpass filter, described
Blue light bandpass filter is arranged between the ultraviolet light collecting lenss group, and the control module electrically connects the xenon flash lamp.
Especially, the photomultiplier transit tube assembly is arranged in a closure.
Especially, the photomultiplier transit tube assembly includes that single photon counting PMT components, mechanical shutter, saturating HONGGUANG light harvesting are saturating
Microscope group and HONGGUANG bandpass filter, the single photon counting PMT components pass sequentially through mechanical shutter, saturating HONGGUANG light collecting lenss group and
HONGGUANG bandpass filter connecting detection interface, the HONGGUANG bandpass filter are arranged between the HONGGUANG light collecting lenss group,
The control module electrical connection single photon counting PMT components and mechanical shutter.
Especially, the both-end of the y-type optical fiber is respectively single silica fibre and multicore silica fibre, the single quartz
Optical fiber connects light cell feedback module, and the multicore silica fibre connects fluorescence excitation interface.
Especially, the mechanical shutter is neighbouring with the cathode plane of the single photon counting PMT components is arranged.
Beneficial effects of the present invention are that the light path for full-automatic time resolution detector is examined compared with prior art
Survey device and there is advantages below：
1) simple structure, changes and can only adopt semi-automatic functional design side because of the labyrinth of light path system in the past
Case, is capable of achieving full-automatic continuous detecting, low cost；With more preferable ductility, large-scale promotion application is suitable to；
2) fluorescence excitation interface and detection interface are arranged vertically, and make exciting light and detection light or not same optical axis, detection
Signal will not produce interference, and accuracy in detection is high；
3) light cell feedback module is provided with for feeding back polishing number of times, is mutually compared with single photon counting PMT components, improve
The accuracy of detection；
Description of the drawings
Fig. 1 is the light path detecting device for full-automatic time resolution detector that the specific embodiment of the invention 1 is provided
Structural representation.
In figure：
1st, light source assembly；11st, xenon flash lamp；12nd, saturating ultraviolet light collecting lenss group；13rd, blue light bandpass filter；2nd, photoelectricity times
Increase tube assembly；20th, single photon counting PMT components；21st, mechanical shutter；22nd, saturating HONGGUANG light collecting lenss group；23rd, HONGGUANG bandpass filter
Piece；3rd, y-type optical fiber；30th, single silica fibre；31st, multicore silica fibre；4th, light cell feedback module；5th, fluorescence excitation interface；
6th, detect interface；7th, darkroom structure.
Specific embodiment
Technical scheme is further illustrated below in conjunction with the accompanying drawings and by specific embodiment.
Refer to shown in Fig. 1, Fig. 1 is the offer of the specific embodiment of the invention 1 for full-automatic time resolution detector
Light path detecting device structural representation.
In the present embodiment, a kind of light path detecting device for full-automatic time resolution detector includes control module, light
Source component 1, photomultiplier transit tube assembly 2, y-type optical fiber 3, light cell feedback module 4, fluorescence excitation interface 5, detection interface 6 and dark
Be used for placing response ware in cell structure 7, the darkroom structure 7, the fluorescence excitation interface 5 and detection interface 6 it is separately positioned
In the darkroom structure 7, and both are arranged vertically, and the photomultiplier transit tube assembly 2 is arranged in a closure.Which includes list
Photon counting PMT component 20, mechanical shutter 21, saturating HONGGUANG light collecting lenss group 22 and HONGGUANG bandpass filter 23, the single photon
Counting PMT components 20 pass sequentially through mechanical shutter 21, saturating HONGGUANG light collecting lenss group 22 and HONGGUANG bandpass filter 23 and connect described
Detection interface 6, the HONGGUANG bandpass filter 23 are arranged between the HONGGUANG light collecting lenss group 22, the control module electricity
Connection single photon counting PMT components 20 and mechanical shutter 21, the mechanical shutter 21 and the single photon counting PMT components 20
Cathode plane is neighbouring to be arranged.
Light beam is divided into two by the single-ended connection light source assembly 1 of the y-type optical fiber 3, and its both-end is respectively single quartz light
Fibre 30 and multicore silica fibre 31, the connection light cell of single silica fibre 30 feedback module 4, the multicore silica fibre 31
Connection fluorescence excitation interface 5, the light source assembly 1 includes xenon flash lamp 11, ultraviolet light collecting lenss group 12 and the filter of blue light band logical thoroughly
Mating plate 13, the blue light bandpass filter 13 are arranged between the ultraviolet light collecting lenss group 12, and the control module is electrically connected
Connect the xenon flash lamp 11.
The xenon flash lamp 11 sends pulse light via saturating ultraviolet light collecting lenss group 12 and blue light band logical in control module
The ultraviolet light of specific wavelength is assembled and is a little coupled with y-type optical fiber 3 by optical filter 13, and via 3 light of y-type optical fiber light, one point is two
Road, is delivered to light cell feedback module 4 via single silica fibre 30 all the way, and the light cell feedback module 4 is used to count simultaneously
Control module is passed to after data again will.Fluorescence excitation interface 5 is delivered to via multicore silica fibre 31 all the way and enters into darkroom knot
Reaction utensil in structure 7.Reactant be stimulated produced by light via detection interface 6 by saturating HONGGUANG light collecting lenss group 22 and red band
Pass filter 23 is by the light of specific wavelength incoming single photon counting PMT components 20.And light is controlled by mechanical shutter 21
The time for passing through.
During use, reaction utensil carries out immunoreation in darkroom structure 7, and fluorescence excitation interface 5 and detection interface 6 are plugged on
In darkroom structure 7, worked by control module control xenon flash lamp 11, the pulsed light for sending by saturating ultraviolet light collecting lenss group 12 and
The blue light bandpass filter 13 of specific wavelength is delivered to fluorescence excitation interface 5 with the y-type optical fiber 3 for coupling and enters reaction utensil, excites
Label in reaction utensil produces fluorescence, and control module control light source assembly 1 quits work, while control module is by mechanical shutter
21 open, label produce fluorescence after testing interface 6 by saturating HONGGUANG light collecting lenss group 22 and specific wavelength HONGGUANG bandpass filter
The photon of specific wavelength is detected by piece 23 by single photon counting PMT components 20, draws detection data, iterative cycles this mistakes
Journey, it is not necessary to manual work, you can fully achieve full-automation.
The above-mentioned light path detecting device design for full-automatic time resolution detector is ingenious, simple for structure, it is complete to be capable of achieving
Automatically, low cost, the reproducible, accuracy of detection are high, are suitable to large-scale promotion application.
Above example simply elaborates the ultimate principle and characteristic of the present invention, and the present invention is not limited by above-mentioned example,
Without departing from the spirit and scope, the present invention also has various change and change, and these changes and change both fall within
In scope of the claimed invention.The claimed scope of the invention is by appending claims and its equivalent thereof.
Claims (6)
1. a kind of light path detecting device for full-automatic time resolution detector, which includes control module, light source assembly, photoelectricity
Multiplication tube assembly, y-type optical fiber, light cell feedback module, fluorescence excitation interface, detection interface and darkroom structure, it is characterised in that
The fluorescence excitation interface and detection interface are separately positioned in the darkroom structure, and both are arranged vertically, the photoelectricity times
Increase tube assembly to be connected with the detection interface, light beam is divided into two by the single-ended connection light source assembly of the y-type optical fiber, its both-end
One end connection fluorescence excitation interface, other end connection light cell feedback module, the control module is electrically connected light sources
Part, photomultiplier transit tube assembly and light cell feedback module, and the y-type optical fiber is silica fibre.
2. the light path detecting device for full-automatic time resolution detector according to claim 1, it is characterised in that institute
Stating light source assembly includes xenon flash lamp, saturating ultraviolet light collecting lenss group and blue light bandpass filter, and the blue light bandpass filter sets
It is placed between the ultraviolet light collecting lenss group, the control module electrically connects the xenon flash lamp.
3. the light path detecting device for full-automatic time resolution detector according to claim 1, it is characterised in that institute
State photomultiplier transit tube assembly to be arranged in a closure.
4. the light path detecting device for full-automatic time resolution detector according to any one of claims 1 to 3, which is special
Levy and be, the photomultiplier transit tube assembly includes single photon counting PMT components, mechanical shutter, saturating HONGGUANG light collecting lenss group and red
Light bandpass filter, the single photon counting PMT components pass sequentially through mechanical shutter, saturating HONGGUANG light collecting lenss group and HONGGUANG band logical
Optical filter connecting detection interface, the HONGGUANG bandpass filter are arranged between the HONGGUANG light collecting lenss group, the control
Module electrically connects single photon counting PMT components and mechanical shutter.
5. the light path detecting device for full-automatic time resolution detector according to claim 1, it is characterised in that institute
The both-end for stating y-type optical fiber is respectively single silica fibre and multicore silica fibre, and the single silica fibre connection light cell is anti-
Feedback module, the multicore silica fibre connect fluorescence excitation interface.
6. the light path detecting device for full-automatic time resolution detector according to claim 4, it is characterised in that institute
Mechanical shutter is stated with the neighbouring setting of the cathode plane of the single photon counting PMT components.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410432610.9A CN104198450B (en) | 2014-08-27 | 2014-08-27 | Light path detection device for full-automatic time resolution detector |
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| CN201410432610.9A CN104198450B (en) | 2014-08-27 | 2014-08-27 | Light path detection device for full-automatic time resolution detector |
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| CN108717052A (en) * | 2018-05-29 | 2018-10-30 | 福建师范大学 | A kind of singlet oxygen luminescent spectrum device for fast detecting of optical fiber transmission |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2252991Y (en) * | 1995-09-15 | 1997-04-23 | 剧庆贺 | Time-resolved fluorescence immunoassay analyzer |
| CN2392181Y (en) * | 1999-08-24 | 2000-08-16 | 王立莉 | Bulb and tube two purpose type light signal immunological testing instrument |
| CN1737538B (en) * | 2005-08-26 | 2012-02-08 | 四川大学 | Fluorescent capillary reaction apparatus and fluorescent capillary analytical method |
| CN100593712C (en) * | 2007-07-06 | 2010-03-10 | 中国科学院上海技术物理研究所 | Laser induction atomic emission spectrum investigating system and method of time resolution |
| US8325337B2 (en) * | 2007-07-13 | 2012-12-04 | Purdue Research Foundation | Time resolved raman spectroscopy |
| CN103245642A (en) * | 2012-02-13 | 2013-08-14 | 上海圭石工业产品设计研发有限公司 | Light path detecting device for full-automatic time-resolved detector |
| CN202929352U (en) * | 2012-09-10 | 2013-05-08 | 昆明理工大学 | Digital real-time holographic shooting system based on fiber |
| CN103728446B (en) * | 2013-12-11 | 2016-02-24 | 天津大学 | Photon counting-type hyperchannel time resolved fluoro-immunoassay system and method for counting |
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