CN103245642A - Light path detecting device for full-automatic time-resolved detector - Google Patents
Light path detecting device for full-automatic time-resolved detector Download PDFInfo
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- CN103245642A CN103245642A CN 201210031787 CN201210031787A CN103245642A CN 103245642 A CN103245642 A CN 103245642A CN 201210031787 CN201210031787 CN 201210031787 CN 201210031787 A CN201210031787 A CN 201210031787A CN 103245642 A CN103245642 A CN 103245642A
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Abstract
The invention relates to a light path detecting device for a full-automatic time-resolved detector. The detecting device comprises a control module, a light source component, a photomultiplier component, a first optical fiber, a second optical fiber and a fluorescence excitation and detection port, wherein the fluorescence excitation and detection port is connected with the light source component and the photomultiplier component through the first optical fiber and the second optical fiber respectively, the control module is in circuit connection with the light source component and the photomultiplier component respectively, the first optical fiber and the second optical fiber are both silica optical fibers, the first optical fiber, the fluorescence excitation and detection port and the second optical fiber are in an integrated structure preferably, the light source component comprises a light source and a first optical filter, the photomultiplier component comprises a photomultiplier, a shutter, a second optical filter and a darkroom structure, and the fluorescence excitation and detection port is connected to the dark room structure in an inserted manner. The light path detecting device provided by the invention is skilful in design, simple in structure, fully automatic, low in cost, good in detection repeatability, high in accuracy, and suitable for large-scale promotion and application.
Description
Technical field
The present invention relates to the time-resolved fluorescence analysis technical field, particularly the light path detection technique field in the full-automatic time resolution detector specifically refers to a kind of for the light path pick-up unit on the full-automatic time resolution detector.
Background technology
Time-resolved fluorescence method is to be that tracer agent comes labelled antigen, antibody, nucleic acid probe or biologically active cell with trivalent rare earth ions (lanthanide series: europium, bismuth, samarium etc.) and huge legendary turtle compound thereof, after trivalent rare earth ions and antigen, the antibody complexing, synergy through reinforcing agent is handled, generation long half time, the fluorescence that fluorescence is strong, thereby improved the sensitivity that detects greatly, accuracy, the method has become a analysis means the most promising in the check of modern biomedical research and clinical ultramicron, is one of the top technology of present ultramicro-analysis and method
The light path pick-up unit is one of key device on the time-resolved fluorescence analytic system, mainly measures the numerical value of fluorescence intensity by photon counting method.The employed light path pick-up unit of time-resolved fluorescence analyser complex structure causes complete machine to have only semi-automatic function at present, has increased personnel cost, and for the repeatability of test, assays such as accuracy rate exert an influence simultaneously.
Therefore, for needs provide a kind of for the light path pick-up unit on the full-automatic time resolution detector, it is simple for structure, it is full-automatic to realize, cost is low, detect good reproducibility, accuracy height.
Summary of the invention
The objective of the invention is to have overcome above-mentioned shortcoming of the prior art, provide a kind of for the light path pick-up unit on the full-automatic time resolution detector, should be used for light path pick-up unit design on the full-automatic time resolution detector ingenious, simple for structure, can realize full-automatic, cost is low, detect good reproducibility, accuracy height, is suitable for large-scale promotion application.
To achieve these goals, of the present invention for the light path pick-up unit on the full-automatic time resolution detector, be characterized in, comprise control module, light source assembly, the photomultiplier assembly, first optical fiber, second optical fiber and fluorescence excitation and detection port, described fluorescence excitation and detection port are connected described light source assembly and described photomultiplier assembly respectively by described first optical fiber and described second optical fiber respectively, described control module circuit respectively connects described light source assembly and described photomultiplier assembly, and described first optical fiber and described second optical fiber are silica fibre.
Preferably, described first optical fiber, described fluorescence excitation and detection port and described second optical fiber are integral structure.
Preferably, described light source assembly comprises light source and first optical filter, and described first optical fiber connects described light source by the described first optical filter light path, and described control module circuit connects described light source.
Preferably, described photomultiplier assembly comprises photomultiplier, shutter and second optical filter, described second optical fiber is connected described photomultiplier by described second optical filter with described fast doors light path successively, and described control module circuit respectively connects described shutter and described photomultiplier.
More preferably, described photomultiplier assembly also comprises the sealing shell, and described photomultiplier, described shutter and described second optical filter all are positioned at described sealing shell, and described second optical fiber wears described sealing shell.
Further, described sealing shell comprises loam cake and housing, and described photomultiplier, described shutter and described second optical filter all are positioned at described housing, and described loam cake lid is located on the described housing, and described second optical fiber wears described loam cake.
More preferably, the cathode plane of described shutter and described photomultiplier is contiguous arranges.
Preferably, describedly also comprise the darkroom structure for the light path pick-up unit on the full-automatic time resolution detector, described fluorescence excitation and detection port are plugged on the structure of described darkroom.
Beneficial effect of the present invention specifically is: of the present inventionly comprise control module for the light path pick-up unit on the full-automatic time resolution detector, light source assembly, the photomultiplier assembly, first optical fiber, second optical fiber and fluorescence excitation and detection port, described fluorescence excitation and detection port are connected described light source assembly and described photomultiplier assembly respectively by described first optical fiber and described second optical fiber respectively, described control module circuit respectively connects described light source assembly and described photomultiplier assembly, described first optical fiber and described second optical fiber are silica fibre, photon from light source through first Optical Fiber Transmission to fluorescence excitation and detection port, mark deposits yields fluorescence in the provocative reaction ware, then by second Optical Fiber Transmission to the photomultiplier assembly, draw check data, design ingenious, simple for structure, can realize full-automatic, cost is low, detect good reproducibility, the accuracy height is suitable for large-scale promotion application.
Description of drawings
Fig. 1 is the structural representation of a specific embodiment of the present invention.
Embodiment
In order more to be expressly understood technology contents of the present invention, describe in detail especially exemplified by following examples.
See also shown in Figure 1, of the present inventionly comprise the control module (not shown) for the light path pick-up unit on the full-automatic time resolution detector, light source assembly 1, photomultiplier assembly 2, first optical fiber 3, second optical fiber 4 and fluorescence excitation and detection port 5, described fluorescence excitation and detection port 5 are connected described light source assembly 1 and described photomultiplier assembly 2 by described first optical fiber 3 respectively with described second optical fiber 4 respectively, described control module circuit respectively connects described light source assembly 1 and described photomultiplier assembly 2,, described first optical fiber and described second optical fiber are silica fibre.
Described first optical fiber 3, described fluorescence excitation and detection port 5 and described second optical fiber 4 can non-Unitaries, see also shown in Figure 1, in specific embodiments of the invention, described first optical fiber 3, described fluorescence excitation and detection port 5 and described second optical fiber 4 are integral structure.
Described light source assembly 1 can adopt any suitable structure, see also shown in Figure 1, in specific embodiments of the invention, described light source assembly 1 comprises light source 11 and first optical filter 12, described first optical fiber 3 connects described light source 11 by described first optical filter, 12 light paths, and described control module circuit connects described light source 11.So-called light path connects and refers to light that light source 11 sends and can see through first optical filter 12 and import first optical fiber 3 into.
Described photomultiplier assembly 2 can adopt any suitable structure, see also shown in Figure 1, in specific embodiments of the invention, described photomultiplier assembly 2 comprises photomultiplier 21, shutter (not shown) and second optical filter 23, described second optical fiber 4 is connected described photomultiplier 21 by described second optical filter 23 with described fast doors light path successively, and described control module circuit respectively connects described shutter and described photomultiplier 21.So-called light path connects the light that refers to from second optical fiber 4 can pass through shutter, second optical filter, 23 arrival photomultipliers 21.
Photomultiplier 21 need place dark surrounds, therefore, see also shown in Figure 1, in specific embodiments of the invention, described photomultiplier assembly 2 also comprises sealing shell 24, described photomultiplier 21, described shutter and described second optical filter 23 all are positioned at described sealing shell 24, and described second optical fiber 4 wears described sealing shell 24.
Described sealing shell 24 can have any suitable structure, see also shown in Figure 1, in specific embodiments of the invention, described sealing shell 24 comprises loam cake 25 and housing 26, described photomultiplier 21, described shutter and described second optical filter 23 all are positioned at described housing 26, described loam cake 25 lids are located on the described housing 26, and described second optical fiber 4 wears described loam cake 25.
Described shutter can be arranged near the correct position of described photomultiplier 21, sees also shown in Figure 1ly, and in specific embodiments of the invention, the cathode plane of described shutter and described photomultiplier 21 is contiguous to be arranged.
The present invention can not comprise the darkroom structure 6 for the reaction utensil reaction, also can comprise, see also shown in Figure 1, in specific embodiments of the invention, describedly also comprise darkroom structure 6 for the light path pick-up unit on the full-automatic time resolution detector, described fluorescence excitation and detection port 5 are plugged on the described darkroom structure 6.
When the present invention uses, reaction utensil carries out immune response in darkroom structure 6, fluorescence excitation and detection port 5 are plugged on the darkroom structure 6, by 11 work of control module control light source, light transmission first optical filter 12 that sends, photon transfers to fluorescence excitation and detection port 5 through first optical fiber, 3 ends, mark deposits yields fluorescence in the provocative reaction ware, and control module control light source 11 quits work.Control module is opened the shutter in the photomultiplier assembly 2 simultaneously, and the fluorescence that label produces transfers to photomultiplier assembly 2 through second optical fiber 4, sees through second optical filter 23, and photon is surveyed by photomultiplier 21, draws the detection data.This process that circulates does not repeatedly need manual work, can realize full-automation fully.
Thereby the invention solves the light path pick-up unit of use complexity in the past and must use artificial filling reagent to influence the accuracy problem of whole testing result, solved the limitation that existing time-resolved fluorescence analytic system has only semi-automatic function.
The present invention is applicable to full-automatic time resolution detector, because employing of the present invention, the present invention has following beneficial effect: (1) is simple in structure, has overturned in the past because of the labyrinth of light path system and can only adopt semi-automatic function design scheme, realizes automation function fully.(2) adopt silica fibre, substitute traditional catoptron, volume is little, transmission security, and light spot energy is evenly distributed, and the bending of energy wide-angle is cheap.
Using the present invention and conventional mirror to detect correlation data please see the following form.
To sum up, of the present invention ingenious, simple for structure for the design of the light path pick-up unit on the full-automatic time resolution detector, can realize full-automatic, cost is low, detect good reproducibility, accuracy height, is suitable for large-scale promotion application.
In this instructions, the present invention is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, instructions and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (8)
1. light path pick-up unit that is used on the full-automatic time resolution detector, it is characterized in that, comprise control module, light source assembly, photomultiplier assembly, first optical fiber, second optical fiber and fluorescence excitation and detection port, described fluorescence excitation and detection port are connected described light source assembly and described photomultiplier assembly respectively by described first optical fiber and described second optical fiber respectively, described control module circuit respectively connects described light source assembly and described photomultiplier assembly, and described first optical fiber and described second optical fiber are silica fibre.
2. according to claim 1 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that described first optical fiber, described fluorescence excitation and detection port and described second optical fiber are integral structure.
3. according to claim 1 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that, described light source assembly comprises light source and first optical filter, described first optical fiber connects described light source by the described first optical filter light path, and described control module circuit connects described light source.
4. according to claim 1 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that, described photomultiplier assembly comprises photomultiplier, shutter and second optical filter, described second optical fiber is connected described photomultiplier by described second optical filter with described fast doors light path successively, and described control module circuit respectively connects described shutter and described photomultiplier.
5. according to claim 4 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that, described photomultiplier assembly also comprises the sealing shell, described photomultiplier, described shutter and described second optical filter all are positioned at described sealing shell, and described second optical fiber wears described sealing shell.
6. according to claim 5 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that, described sealing shell comprises loam cake and housing, described photomultiplier, described shutter and described second optical filter all are positioned at described housing, described loam cake lid is located on the described housing, and described second optical fiber wears described loam cake.
7. according to claim 4ly it is characterized in that the contiguous setting of the cathode plane of described shutter and described photomultiplier for the light path pick-up unit on the full-automatic time resolution detector.
8. according to claim 1 for the light path pick-up unit on the full-automatic time resolution detector, it is characterized in that, describedly also comprise the darkroom structure for the light path pick-up unit on the full-automatic time resolution detector, described fluorescence excitation and detection port are plugged on the structure of described darkroom.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210031787 CN103245642A (en) | 2012-02-13 | 2012-02-13 | Light path detecting device for full-automatic time-resolved detector |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210031787 CN103245642A (en) | 2012-02-13 | 2012-02-13 | Light path detecting device for full-automatic time-resolved detector |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104181140A (en) * | 2014-08-27 | 2014-12-03 | 江苏锐汗德医疗科技有限公司 | Detection disc applied to full-automatic tubular time resolution detector |
| CN104198450A (en) * | 2014-08-27 | 2014-12-10 | 江苏锐汗德医疗科技有限公司 | Light path detection device for full-automatic time resolution detector |
-
2012
- 2012-02-13 CN CN 201210031787 patent/CN103245642A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104181140A (en) * | 2014-08-27 | 2014-12-03 | 江苏锐汗德医疗科技有限公司 | Detection disc applied to full-automatic tubular time resolution detector |
| CN104198450A (en) * | 2014-08-27 | 2014-12-10 | 江苏锐汗德医疗科技有限公司 | Light path detection device for full-automatic time resolution detector |
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Application publication date: 20130814 |