CN204347027U - A kind of optical fiber array column immunofluorescence assay device - Google Patents
A kind of optical fiber array column immunofluorescence assay device Download PDFInfo
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- CN204347027U CN204347027U CN201420791175.4U CN201420791175U CN204347027U CN 204347027 U CN204347027 U CN 204347027U CN 201420791175 U CN201420791175 U CN 201420791175U CN 204347027 U CN204347027 U CN 204347027U
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Abstract
The utility model provides a kind of optical fiber array column immunofluorescence assay device, specifically comprises: microcontroller, Fiberoptic Array Sensing device, stepper motor, reagent card bracket, reagent card; Described reagent card, for holding test substance; Described reagent card bracket is used for propping up described reagent card; Described stepper motor is connected with described reagent card bracket, moves for driving described reagent card bracket; Described microcontroller is connected with described stepper motor, moves to assigned address for reagent card bracket described in control step driven by motor; Described Fiberoptic Array Sensing device is connected with microcontroller, and for scanning reagent card, and the data of test substance scanning obtained transfer to microcontroller; With this, achieve the high sensitivity, the high flux that detect at the scene, not only the fiber array immunofluorescence assay that quantitatively detects of simple to operate, good stability but also can be used for, can be widely used in the detection of food security, residue of veterinary drug.
Description
Technical field
The utility model relates to detection of veterinary drugs in food, food security supervision and clinical analysis of diagnosis field, for fluorescence immune chromatography quantitative test.Specifically comprise a kind of optical fiber array column high throughput assay systems, can the concentration of measured object in on-the-spot Quantitative detection sample.
Background technology
Immunological technique comes across the eighties, it is a kind of quick diagnosis technology, its principle is a certain zone special antibody being first fixed on nitrocellulose filter, after sample urine or serum are immersed in cellulose nitrate one end of this drying, due to capillarity, sample will move forward along this film, when moving to the region being fixed with antibody, in sample corresponding antigen namely with this antibody generation specific binding, if this region can be made to show certain color with immune colloid gold or Immunoperoxidase Staining, thus realize specific immunodiagnosis.
At present, the colloidal gold technique of Site Detection is can be used in detection of veterinary drugs in food, food security supervision field.This detection technique is marked at by antigen-antibody on fiber chromatographic material to form detection zone, makes sample swimming on chromatography strip by capillary action.The receptor antibody that sample determinand and chromatographic material are cured or antigen generation specificity compatible reaction, sample determinand in detection zone enrichment, and forms colour band.This colour band reads by chart scanner or passes through the interpretation of people's naked eyes.
The chart scanner of the method is based on CCD imaging technique, yin and yang attribute differentiation is carried out by the depth of detection zone and quality control region color, but because colloid gold label thing is unstable, same thing to be checked changes greatly in different colloidal gold strip detection zones color, and the contrast of this coloured panel and chromatography strip background is low cannot quantitatively detect and high flux detects.
Utility model content
The utility model object is defect for prior art and deficiency, provides a kind of high flux, high sensitivity, simple to operate, good stability, can be used for the fiber array immunofluorescence assay system that quantitatively detects.
For solving the problems of the technologies described above, the utility model provides a kind of optical fiber array column immunofluorescence assay device, and described determinator comprises: microcontroller, Fiberoptic Array Sensing device, stepper motor, reagent card bracket, reagent card; Described reagent card, for holding the test substance with fluorescence antibody; Described reagent card bracket is used for propping up described reagent card; Described stepper motor is connected with described reagent card bracket, moves for driving described reagent card bracket; Described microcontroller is connected with described stepper motor, moves to assigned address for reagent card bracket described in control step driven by motor; Described Fiberoptic Array Sensing device is connected with microcontroller, for scanning reagent card, and the test result that scanning obtains is transferred to microcontroller output.
In technique scheme, described Fiberoptic Array Sensing device comprises: incident optical module and reception optic module; Described incident optical module by the fiber-optic illuminated fluorescence antibody excited on reagent card in test substance, in order to excite the light signal on test substance; Described reception optic module, for receiving the fluorescence signal on reagent card.
In technique scheme, described incident optical module to be connected with laser tube by optical fiber to be made; Described laser tube is used for Emission Lasers signal; Described optical fiber is used for described laser signal to derive.
In technique scheme, described reception light source module to be connected with photoelectric sensor by optical fiber, optical filter to be made; Described optical fiber is for receiving lead-in light signal; Described optical filter is for filtering the veiling glare in optical fiber lead-in light signal; Described photoelectric sensor is used for the light signal after by filtration veiling glare and converts electronic signal to.
In technique scheme, described Fiberoptic Array Sensing device forms by organizing optical fiber sensor more, and described optical fiber sensor is made up of incident optical module and reception optic module.
In technique scheme, described reagent card comprises: reference band, well and many detection zone.
In technique scheme, described microcontroller also connects a touch control display module, and described touch-control module drives described reagent card bracket to move to assigned address for opening microprocessor controls stepper motor, and receives the test result of microcontroller output and show.
In technique scheme, described touch control display module also connects a storage module, and described storage module is for storing the test result of described reception.
In technique scheme, described touch control display module also connects an embedded printer, for the test result received described in printing.
Advantageous Effects of the present utility model is: the utility model have employed the Fiberoptic Array Sensing device be made up of incident light module and reception optical module, achieve array scanning, and achieve a reagent card multi-ribbon detection and the detection of many group reagents card multi-ribbon, effectively improve the detection flux of detection system, improve work efficiency.The use of optical fiber technology, makes the accurate positioning of incident light and reception light prevent ambient light and material to incident light and the interference accepting light, effectively improves the noise when detection sensitivity of system.The utility model devises reference type multiparameter fluorescence immunoassay reagent card, by adding signal to noise ratio (S/N ratio) in the use of antibody (antigen) upper mark fluorescent material fluorescent coumarin dye or the general fluorescent nanometer microsphere of other business and black floor, make the numerical difference of detection background and actual detection signal apart from maximizing, the sensitivity of the detection of increase and stability.Reagent card adds reference detection zone, and the antibody antigen that reference band uses is not combined with measured object, and quantitative mark, and optical output signal is stablized, and can to compensate in sample liquid not clear material to the interference of testing.The detected value of reference band participates in detection zone computing, makes final testing result reliable and stable.
Accompanying drawing explanation
Fig. 1 is schematic diagram of the present utility model
Fig. 2 is optical fiber component schematic diagram of the present utility model
Fig. 3 is reference type multiparameter fluorescence immunoassay reagent card
Fig. 4 is reference type multiparameter fluorescence immunoassay reagent card
Embodiment
For making the object of the utility model embodiment, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the utility model embodiment, technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
The utility model provides a kind of optical fiber array column immunofluorescence assay device, and described determinator comprises: microcontroller, Fiberoptic Array Sensing device, stepper motor, reagent card bracket, reagent card; Described reagent card, for holding the test substance with fluorescence antibody; Described reagent card bracket is used for propping up described reagent card; Described stepper motor is connected with described reagent card bracket, moves for driving described reagent card bracket; Described microcontroller is connected with described stepper motor, moves to assigned address for reagent card bracket described in control step driven by motor; Described Fiberoptic Array Sensing device is connected with microcontroller, for scanning reagent card, and the test result that scanning obtains is transferred to microcontroller output.
In the above-described embodiments, described Fiberoptic Array Sensing device comprises: incident optical module and reception optic module; Described incident optical module by the fiber-optic illuminated fluorescence antibody excited on reagent card in test substance, in order to excite the light signal on test substance; Described reception optic module, for receiving the fluorescence signal on reagent card.
In the above-described embodiments, described microcontroller also connects a touch control display module, and described touch-control module drives described reagent card bracket to move to assigned address for opening microprocessor controls stepper motor, and receives the test result of microcontroller output and show.
In the above-described embodiments, described touch control display module also connects a storage module, and described storage module is for storing the test result of described reception.
In the above-described embodiments, described touch control display module also connects an embedded printer, for the test result received described in printing.
About concrete structure and the annexation of above-described embodiment, please refer to shown in Fig. 1-Fig. 2, optical fiber array column immunofluorescence assay device comprises microcontroller 106, Fiberoptic Array Sensing device 103, incident light source module 104, receives light source module 105, stepper motor 102, reagent card bracket 101, touch display module 108, storage module 109, embedded printer 110.
Wherein reagent card bracket 101 is placed on stepper motor 102, and microcontroller 106 control step motor 102 moves, and drives the reagent card bracket 101 settled thereon to assigned address in stepper motor 102 motion process; After this reagent card bracket 101 arrives assigned address, the test substance held in Fiberoptic Array Sensing device 103 pairs of reagent card brackets 101 detects and exports the testing result obtained after detection to microcontroller 106; Microcontroller 106 exports the test result received to touch display module 108 display translation.
Also provide a storage module and embedded printer in the application one preferred embodiment, as shown in Figure 1, this storage module 109 is all connected in touch display module 108 with embedded printer 110, for test result being stored and printing.
In technique scheme, described incident optical module to be connected with laser tube by optical fiber to be made; Described laser tube is used for Emission Lasers signal; Described optical fiber is used for described laser signal to derive.Wherein, described reception light source module to be connected with photoelectric sensor by optical fiber, optical filter and to make; Described optical fiber is for receiving lead-in light signal; Described optical filter is for filtering the veiling glare in optical fiber lead-in light signal; Described photoelectric sensor is used for the light signal after by filtration veiling glare and converts electronic signal to; Described Fiberoptic Array Sensing device forms by organizing optical fiber sensor more, and described optical fiber sensor is made up of incident optical module and reception optic module.
The application is by the fluorescence signal of the point-to-point acquisition testing liquid of many group optical fiber sensors in Fiberoptic Array Sensing device, make the detection and location of fluorescence accurately also prevent ambient light and material to incident light and the interference accepting light with this, effectively improve the noise when detection sensitivity of system.Specifically can with reference to shown in figure 2, Fiberoptic Array Sensing device 103 is made up of incident optical module 104 and these two fundamental elements of reception optic module 105; Wherein incident optical module 104 is made up of laser tube 201 and optical fiber 202, in real work, by laser tube 201 utilizing emitted light, is sent to region to be measured by optical fiber 202.To excite the light signal of test substance; And receive optic module 105 by optical fiber 203, optical filtering 204 and photoelectric sensor 205 three and form; After above-mentioned incident optical module inspires the light signal of test substance, this light signal is brought into optical filtering 204 place by optical fiber 203, the light signal of importing is removed veiling glare and is exported photoelectric sensor 205 to by optical filtering 204, and photoelectric sensor 205 converts the light signal of reception to electronic signal and exports.
Because the utility model have employed the Fiberoptic Array Sensing device be made up of incident light module and reception optical module, achieve array scanning, therefore, effectively improve the detection flux of detection system, improve work efficiency.The use of optical fiber technology, makes the accurate positioning of incident light and reception light prevent ambient light and material to incident light and the interference accepting light, effectively improves the noise when detection sensitivity of system.
In another embodiment of the application, described reagent card comprises: reference band, well and many detection zone.
Specifically please refer to shown in Fig. 3-Fig. 4; Reference type multiparameter fluorescence immunoassay reagent card by reagent card shell 301,401, detection zone 303,403 can be wall scroll also can be many, reference band 304,404, well 302,402, cellulose acetate film forms; The making of reference type multiparameter fluorescence immunoassay reagent card: the detection zone 303,403 with peristaltic pump, the antibody antigen being used for detecting being uniformly coated on the cellulose acetate film of band liner plate, reference antibody is uniformly coated on reference band 304,404, after drying, puts into reagent cartridge 301,401.
Because the utility model devises reference type multiparameter fluorescence immunoassay reagent card, by adding signal to noise ratio (S/N ratio) in the use of antibody (antigen) upper mark fluorescent material fluorescent coumarin dye or the general fluorescent nanometer microsphere of other business and black floor, make the numerical difference of detection background and actual detection signal apart from maximizing, the sensitivity of the detection of increase and stability.Reagent card adds reference detection zone, and the antibody antigen that reference band uses is not combined with measured object, and quantitative mark, and optical output signal is stablized, and can to compensate in sample liquid not clear material to the interference of testing.The detected value of reference band participates in detection zone computing, makes final testing result reliable and stable.And many detection zone 303,403 are set also effectively achieve reagent card multi-ribbon and to detect and many group reagents card multi-ribbon detects, not only effectively improve the detection flux of detection system, also improve work efficiency.
Embodiment the application provided applies in time service, can obtain good effect, and operation is comparatively easy, and concrete steps are as follows: sample liquid and fluorescence antibody are mixed, and add in the well 302,402 of reagent card 301 or 401, wait for 10 minutes.Reagent card 301 or 401 is put into the reagent card bracket 101 of optical fiber array column immunofluorescence assay instrument.Open microcontroller by touch display screen 108, microcontroller 106 is after the instruction receiving touch display screen 108, and control step motor 102 brings reagent card bracket into detector precalculated position.The reagent card that microprocessor controls Fiberoptic Array Sensing device 103 pairs of stepper motors drag simultaneously scans.The detection zone 303,304,403,404 that the laser beam that laser tube 201 in incident optical module 104 launches wavelength stabilization irradiates reagent card by optical fiber 202 inspires fluorescence, this fluorescence is received optic module 105 and accepts, optical filtering 204 elimination veiling glare is conducted to by optical fiber 203, light signal is changed into electronic signal by photoelectric sensor 205, obtains test result; Test result exports by microcontroller 106, can be presented on touch display screen 108, can be stored on storage module 109, also by embedded printer 110 print result.
Assembling of the present utility model: reagent card bracket, stepper motor 102, Fiberoptic Array Sensing device 103 are put into 260 × 230 × 150mm tool housing after being connected by control circuit with microcontroller, touch display screen 108 printer 110 is arranged on the front panel of shell, data transmission interface is housed after shell to be connected with computing machine, composition the utility model.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (9)
1. an optical fiber array column immunofluorescence assay device, is characterized in that, described determinator comprises: microcontroller, Fiberoptic Array Sensing device, stepper motor, reagent card bracket, reagent card; Described reagent card, for holding the test substance with fluorescence antibody;
Described reagent card bracket is used for propping up described reagent card;
Described stepper motor is connected with described reagent card bracket, moves for driving described reagent card bracket;
Described microcontroller is connected with described stepper motor, moves to assigned address for reagent card bracket described in control step driven by motor;
Described Fiberoptic Array Sensing device is connected with microcontroller, for scanning reagent card, and the test result that scanning obtains is transferred to microcontroller output.
2. optical fiber array column immunofluorescence assay device according to claim 1, it is characterized in that, described Fiberoptic Array Sensing device comprises: incident optical module and reception optic module;
Described incident optical module by the fiber-optic illuminated fluorescence antibody excited on reagent card in test substance, in order to excite the light signal on test substance;
Described reception optic module, for receiving the fluorescence signal on reagent card.
3. optical fiber array column immunofluorescence assay device according to claim 2, is characterized in that, described incident optical module to be connected with laser tube by optical fiber to be made;
Described laser tube is used for Emission Lasers signal;
Described optical fiber is used for described laser signal to derive.
4. optical fiber array column immunofluorescence assay device according to claim 2, is characterized in that, described reception light source module to be connected with photoelectric sensor by optical fiber, optical filter to be made;
Described optical fiber is for receiving lead-in light signal;
Described optical filter is for filtering the veiling glare in optical fiber lead-in light signal;
Described photoelectric sensor is used for the light signal after by filtration veiling glare and converts electronic signal to.
5. the optical fiber array column immunofluorescence assay device according to claim 3 or 4, it is characterized in that, described Fiberoptic Array Sensing device forms by organizing optical fiber sensor more, and described optical fiber sensor is made up of incident optical module and reception optic module.
6. optical fiber array column immunofluorescence assay device according to claim 1, it is characterized in that, described reagent card comprises: reference band, well and many detection zone.
7. optical fiber array column immunofluorescence assay device according to claim 1, it is characterized in that, described microcontroller also connects a touch control display module, described touch-control module drives described reagent card bracket to move to assigned address for opening microprocessor controls stepper motor, and receives the test result of microcontroller output and show.
8. optical fiber array column immunofluorescence assay device according to claim 7, it is characterized in that, described touch control display module also connects a storage module, and described storage module is for storing the test result of described reception.
9. optical fiber array column immunofluorescence assay device according to claim 7, it is characterized in that, described touch control display module also connects an embedded printer, for the test result received described in printing.
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| CN201420791175.4U CN204347027U (en) | 2014-12-12 | 2014-12-12 | A kind of optical fiber array column immunofluorescence assay device |
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| CN201420791175.4U CN204347027U (en) | 2014-12-12 | 2014-12-12 | A kind of optical fiber array column immunofluorescence assay device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107102129A (en) * | 2017-05-31 | 2017-08-29 | 上海理工大学 | A kind of ELIASA |
| CN109596604A (en) * | 2018-12-24 | 2019-04-09 | 东北师范大学 | A kind of adjustable chemiluminescence Fiber imunosensor of the range of linearity and application |
| CN111413510A (en) * | 2019-01-08 | 2020-07-14 | 深圳迎凯生物科技有限公司 | Device control method, device, detection device and storage medium |
-
2014
- 2014-12-12 CN CN201420791175.4U patent/CN204347027U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107102129A (en) * | 2017-05-31 | 2017-08-29 | 上海理工大学 | A kind of ELIASA |
| CN109596604A (en) * | 2018-12-24 | 2019-04-09 | 东北师范大学 | A kind of adjustable chemiluminescence Fiber imunosensor of the range of linearity and application |
| CN109596604B (en) * | 2018-12-24 | 2021-07-09 | 东北师范大学 | Chemiluminescence optical fiber immunosensor with adjustable linear range and application thereof |
| CN111413510A (en) * | 2019-01-08 | 2020-07-14 | 深圳迎凯生物科技有限公司 | Device control method, device, detection device and storage medium |
| CN111413510B (en) * | 2019-01-08 | 2024-03-19 | 深圳迎凯生物科技有限公司 | Device control method, device, detection device and storage medium |
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