CN206945538U - The high quick fluorescent chromatographic device of group of the lanthanides - Google Patents
The high quick fluorescent chromatographic device of group of the lanthanides Download PDFInfo
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- CN206945538U CN206945538U CN201720742591.9U CN201720742591U CN206945538U CN 206945538 U CN206945538 U CN 206945538U CN 201720742591 U CN201720742591 U CN 201720742591U CN 206945538 U CN206945538 U CN 206945538U
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Abstract
The utility model discloses a kind of high quick fluorescent chromatographic device of group of the lanthanides, including excitation source, mobile device, optical splitter, light source optical path, fluorescence light path, optical-electrical converter, circuit arrangement and communicator, the mobile device includes a stepper motor and the transmission mechanism driven by stepper motor, chromatography detection card is placed on transmission mechanism, the corresponding chromatography detection card of excitation source is set, optical splitter is set between excitation source and chromatography detection card, light source optical path is set between optical splitter and chromatography detection card, fluorescence light path is set between optical splitter and optical-electrical converter, excitation source, optical-electrical converter and mobile device are controlled by circuit arrangement, the excitation source is that can send the excitation source more than 400nm wavelength exciting lights, circuit arrangement drives communicator, communicator connects terminal.The utility model had not only ensured that background fluorescence was low but also had ensured that fluorescence signal is strong, and then improved signal to noise ratio.
Description
Technical field
The utility model belongs to chromatography detection technique field, more particularly to a kind of high quick fluorescent chromatographic device of group of the lanthanides.
Background technology
To meet that POCT detects the application of (point-of-care testing), gold mark chromatography quick determination method immediately
It is widely used, with the demand to quantitative detection, occurs the microballoon of various replacement collaurums in market, be concentrated mainly on glimmering
Light microballoon and time resolution microballoon.
Common fluorescent microsphere has stronger fluorescence signal, substantially increases the detection signal intensity of chromatography strip, solves
The problem of collaurum judges to segment using color.And time-resolved fluorescence has higher specificity, using avoiding swashing
Fluoresce and excite light irradiation reasons for its use fluorescence in irradiation fluorescent microsphere moment, the method for delay collection fluorescence signal, avoidance
Interference, greatly improve signal to noise ratio.However, because time-resolved fluorescence is after excitation source closing, fluorescence intensity sharp-decay,
It is i.e. so-called to be decayed in a manner of exponential curve.Although that is, after delay, ambient interferences fluorescence does not almost have, when
Between resolved fluorometric it is also very weak.Consequently, it is possible to the signal to noise ratio improved is also just relatively limited.
The ambient interferences for how solving common fluorescent are big and contradictory problems that time-resolved fluorescence signal is weak become the neck
The direction that field technique personnel make great efforts.
Utility model content
The purpose of this utility model is just to provide a kind of high quick fluorescent chromatographic device of group of the lanthanides, can be fully solved above-mentioned existing skill
The weak point of art.
The purpose of this utility model is realized by following technical proposals：
A kind of high quick fluorescent chromatographic device of group of the lanthanides, including excitation source, mobile device, optical splitter, light source optical path, fluorescence light
Road, optical-electrical converter, circuit arrangement and communicator, the mobile device include a stepper motor and driven by stepper motor
Dynamic transmission mechanism, chromatography detection card is placed on transmission mechanism, the corresponding chromatography detection card of excitation source is set, in excitation source
Optical splitter is set between chromatography detection card, light source optical path is set between optical splitter and chromatography detection card, in optical splitter and photoelectricity
Fluorescence light path is set between converter, and excitation source, optical-electrical converter and mobile device are controlled by circuit arrangement, and circuit arrangement drives
Dynamic communicator, communicator connection terminal.
Preferably, the excitation wavelength that the excitation source is sent is less than 550nm more than 400nm.
Preferably, it is the long-life that the excitation source, which excites fluorescence caused by the fluorescent marker that chromatography detection blocks,
Fluorescence.
Preferably, the excitation source is LED light source.
Preferably, the circuit arrangement include programmable logic controller (PLC), amplifier, A/D converter, light source switch,
Analog switch and bluetooth, the amplifier, analog switch and A/D converter Electricity Federation successively, A/D converter and bluetooth are with that can compile
Journey logic controller Electricity Federation, amplifier and optical-electrical converter Electricity Federation, light source switch and excitation source Electricity Federation, Programmable logical controller
Device connects mobile device.
Preferably, the circuit arrangement includes USB functional modules, circuit arrangement passes through USB functional modules or bluetooth
Connect terminal.
Preferably, the terminal connection internet, terminal through internet obtains the standard curve of detection sample, and will
Internet is arrived in testing result storage.
Prompt fluorescence detection technique is to begin to detect fluorescence signal when exciting light is closed, and now fluorescence signal includes mark
Remember the interference signals such as fluorescent microsphere fluorescence, impurity fluorescence, exciting light, scattering light, and disturbed with unstable pulse signal.It is logical
It is usually used in the analysis of non-trace fluorescent material.
Fluorescence longevity of the time delay selection of time-resolved fluorescence chromatography collection fluorescence signal substantially according to fluorescent material
Life is set, because fluorescence intensity is with exponential damping speed sharp-decay.Fluorescence signal now is very weak, it is necessary to glimmering by improving
The precision of light instrument or the enlargement ratio of optical-electrical converter are realized.Time-resolved fluorescence detection and analysis sensitivity is high, is applicable
In the fluorescent material detection method of measurement.
Excitation source is opened into close moment defined in the present invention, fluorescent microsphere be excited caused by fluorescence be instantaneous glimmering
Light；After excitation source is closed, fluorescence caused by fluorescent microsphere is time-resolved fluorescence.Fluorescent microsphere in excitation source opening process
Caused prompt fluorescence signals intensity level is a definite value, and time-resolved fluorescence signal strength values be one with the time increase and
The value quickly reduced.
In fluorescent chromatographic method, the material such as serum, toxin, mould and cellulase, NC films (is referred to as impurity in sample
Fluorescent material), impurity fluorescence will be also produced under excitation, ultraviolet excitation is more glimmering than excited by visible light, caused impurity
Light intensity.The present apparatus uses (wavelength is 400nm -480nm ± 5%) excited by visible light, and the time-resolved fluorescence compared to routine uses
340-360nm uv excitation lights, the interference of caused impurity fluorescence are drastically reduced, and are possessed during gathering delay 0-200uS more
The condition of strong time-resolved fluorescence signal, avoid the extremely weak time-resolved fluorescence signal after collection delay 200-400uS.
Compared with prior art, the beneficial effects of the utility model are：It is simple in construction, it is reasonable in design, it is greatly lowered
Background fluorescence, improves fluorescence intensity, and shortens detection time, not only ensured that background fluorescence is low but also ensure fluorescence signal it is strong and then
Improve signal to noise ratio.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of circuit arrangement in Fig. 1；
Fig. 3 is the high quick fluorescence decay curve of group of the lanthanides.
Embodiment
The utility model is further described with reference to specific embodiments and the drawings.
As depicted in figs. 1 and 2, the high quick fluorescent chromatographic device of a kind of group of the lanthanides, including excitation source 1, mobile device 2, light splitting
Device 3, light source optical path 4, fluorescence light path 5, optical-electrical converter 6, circuit arrangement 7 and communicator 8, the mobile device 2 include one
Individual stepper motor and the transmission mechanism driven by stepper motor, chromatography detection card 9, excitation source 1 are placed on transmission mechanism
Corresponding chromatography detection card 9 is set, and sets optical splitter 3 between excitation source 1 and chromatography detection card 9, optical splitter 3 detects with chromatography
Light source optical path 4 is set between card 9, fluorescence light path 5 is set between optical splitter 3 and optical-electrical converter 6, excitation source 1, photoelectricity turn
Parallel operation 6 and mobile device 2 are controlled by circuit arrangement 7, and circuit arrangement 7 drives communicator 8, and communicator 8 connects terminal.It is described
Excitation source 1 is that can send the excitation source more than 420nm wavelength exciting lights.When stepper motor is driven by circuit arrangement 7, pass
Motivation structure can drive chromatography detection card 9 to move left and right, and have the function that scanning.The terminal is smart mobile phone or PC, is led to
Cross terminal and realize detection display and report.The mobile device 2, optical splitter 3, light source optical path 4, fluorescence light path 5, optical-electrical converter
6 and communicator 8 be prior art, will not be repeated here.
As preferable technical scheme, the excitation source 1 is LED light source, and the excitation light wave that excitation source 1 is sent is grown up
It is less than 550nm in 420nm.
At work, exciting light caused by excitation source 1 is glimmering by the group of the lanthanides on the irradiation chromatography detection of light source optical path 4 card 9
Light microballoon, lanthanide fluoro microballoon is excited to produce fluorescence, the fluorescence is long-life phosphors, and fluorescence enters fluorescence light by optical splitter 3
Road 5 focuses on optical-electrical converter 6, and fluorescence signal is converted into electric signal by optical-electrical converter 6, and electric signal is sent into circuit arrangement 7, electricity
Road device 7 controls the movement of chromatography detection card 9 by mobile device 2.The excitation source 1 is closed after opening 10-100us.
Circuit arrangement 7 gathers and recorded the lanthanide fluoro microballoon that chromatography detection card 9 excites while excitation source 1 is closed
Fluorescence signal intensity value, and more than continuous sampling 400us, then circuit arrangement 7 the fluorescence signal intensity value collected is asked
Average value seeks weighted average, obtains fluorescence signal intensity value of the chromatography detection card 9 in the position, then circuit arrangement 7 is again
Chromatography detection is controlled to block the collection that 9 movements carry out subsequent point fluorescence signal intensity value by mobile device 2, until completing whole layer
The detection of analysis detection card 9.
Cellulose membrane, dust, albumen and impurity on chromatography detection card 9 etc. can produce background under the irradiation of exciting light
Fluorescence, especially under the exciting of the exciting light of 340-360nm wavelength, these background fluorescences can be very strong, but background fluorescence
Compared to the time-resolved fluorescence of long-life, the fluorescence that background fluorescence excites after excitation source closing can decline at faster speed
Subtract, can be typically quenched in 200us-300us or so, background fluorescence.The present apparatus uses the exciting light of 420nm wavelengths above, is produced
Raw background fluorescence will be reduced largely, and the fluorescence that then background fluorescence excites after exciting light closing can decline at faster speed
Subtract, can be typically quenched in 50us-100us or so, background fluorescence.
When the fluorescent microsphere of chromatography detection card uses excitation wavelength to be more than the lanthanide fluoro microballoon that 420nm is less than 550nm
When, after excitation source is closed, background fluorescence is weaker in itself, can be quenched within the extremely short time, then can be long-lived by collection
The time of life fluorescence shifts to an earlier date significantly, greatly improving for the fluorescence intensity for meaning to collect in advance of time, reaches and both ensures to carry on the back
Scape fluorescence is low and ensures that fluorescence signal is strong and then improves the purpose of signal to noise ratio.
By the way of continuous acquisition fluorescence, it starts circuit arrangement 7 of the present utility model in exciting light close moment
Continuous acquisition fluorescence in the 400us times, and the weighted average of fluorescence signal intensity value is sought, delay weights over time are gradual
Increase, improve weight of the long-life phosphors signal in average value by delay time, finally obtain the average value of fluorescence intensity.
Fig. 3 is illustrated and the change procedure of fluorescence intensity, circuit arrangement 7 gather at the beginning of between point for excitation source close
Moment (i.e. turning off the light the moment in figure).Characteristic according to fluorescence with time exponential damping, the fluorescence intensity that the moment gathers earlier are
The time-resolved fluorescence of collection after significantly larger than, this needs have benefited from the excitation wavelength for being more than 420nm using wavelength, and
The longer fluorescence excited of wavelength can be stronger.
In the present embodiment, the testing result F of the high quick fluorescence of group of the lanthanides of any one upper position of chromatography card is：
F=∑s [f (t) × t]/N
F (t) is electric signal of the optical-electrical converter in the fluorescent intensity collected at different moments in formula, and t is in exciting light
Sampling number after the closing of source, it is 0 to 400us, time interval 1us, is total to times of collection N=400 times.Collection result is with prolonging
When increase, weights are gradually increased, if necessary may the increase of weights not to scale (NTS), the present embodiment do not provide.
Referring to Fig. 2, the circuit arrangement 7 include programmable logic controller (PLC) 7-1, amplifier 7-2, A/D converter 7-3,
Light source switch 7-4, analog switch 7-5 and bluetooth 7-6, the amplifier 7-2, analog switch 7-5 and A/D converter 7-3 are successively
Electricity Federation, A/D converter 7-3 and bluetooth 7-6 with programmable logic controller (PLC) 7-1 Electricity Federations, amplifier 7-2 and optical-electrical converter 6
Electricity Federation, light source switch 7-4 and the Electricity Federation of excitation source 1, programmable logic controller (PLC) 7-1 connections mobile device 2.The circuit arrangement
7 also include USB functional module 7-7, and circuit arrangement 7 passes through USB functional modules 7-7 or bluetooth 7-6 connection terminals 7-8.It is described
Terminal 7-8 connections internet, terminal 7-8 obtain the standard curve of detection sample by internet, and testing result storage is arrived
Internet.Terminal 7-8 uses smart mobile phone herein.
Under programmable logic controller (PLC) 7-1 (FPGA) SECO, the transmission mechanism of control stepper motor driving makes
Chromatography detection card 9 steppings movement, fluorescence signal caused by convenient each point position on chromatography detection card are acquired.Then,
Excitation source 1 is opened by light source switch 7-4, after irradiating 30us, closes excitation source 1.Chromatography detection card 9 is in excitation source 1
Irradiation after, fluorescence caused by fluorescent microsphere enters optical-electrical converter 6 on chromatography detection card 9, produces electric signal, electric signal process
Enter analog switch 7-5 after amplifier 7-2 processing, and under programmable logic controller (PLC) 7-1 (FPGA) SECO, by adopting
Sample is realized is converted to data signal f (t) by electric signal feeding high speed analog-digital conversion A/D converter 7-3, then f (t) is weighted flat
After, the fluorescence intensity data of this position is sent to smart mobile phone by bluetooth, by the application program logarithm in smart mobile phone
According to being shown and handled.Then the movement chromatography detection of mobile device 2 card 9 is driven, carries out the fluorescence signal acquisition of next position,
Until the detection of the lanthanide fluoro of whole chromatography detection card 9 is completed in scanning.
The circuit arrangement 7 chronologically carries out the Scanning Detction data of fluorescence intensity to chromatography detection card 9, passes through bluetooth 7-6
The scan data of detection means is passed to smart mobile phone, the APP application programs in smart mobile phone calculate the upper C lines of chromatography card and T
The fluorescence signal intensity of line.Smart mobile phone obtains the preset standard curve of kit, root by connecting internet from internet
Block upper C lines and the fluorescence signal intensity of T lines and the contrast relationship of standard curve according to chromatography, calculate detected target detection thing
Content.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
All any modification, equivalent and improvement made within the spirit and principle of utility model etc., should be included in the utility model
Protection domain within.
Claims (7)
1. a kind of high quick fluorescent chromatographic device of group of the lanthanides, including excitation source, mobile device, optical splitter, light source optical path, fluorescence light
Road, optical-electrical converter, circuit arrangement and communicator, the mobile device include a stepper motor and driven by stepper motor
Dynamic transmission mechanism, chromatography detection card is placed on transmission mechanism, the corresponding chromatography detection card of excitation source is set, in excitation source
Optical splitter is set between chromatography detection card, light source optical path is set between optical splitter and chromatography detection card, in optical splitter and photoelectricity
Fluorescence light path is set between converter, and excitation source, optical-electrical converter and mobile device are controlled by circuit arrangement, and circuit arrangement drives
Dynamic communicator, communicator connection terminal.
2. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 1, it is characterised in that：What the excitation source was sent swashs
Emission wavelength is less than 550nm more than 400nm.
3. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 1, it is characterised in that：The excitation source excites chromatography
Fluorescence caused by fluorescent marker on detection card is long-life phosphors.
4. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 1, it is characterised in that：The excitation source is LED light
Source.
5. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 1, it is characterised in that：The circuit arrangement includes compiling
Journey logic controller, amplifier, A/D converter, light source switch, analog switch and bluetooth, the amplifier, analog switch and A/
Electricity Federation, A/D converter and bluetooth are electric with optical-electrical converter with programmable logic controller (PLC) Electricity Federation, amplifier successively for D converters
Connection, light source switch and excitation source Electricity Federation, programmable logic controller (PLC) connection mobile device.
6. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 5, it is characterised in that：The circuit arrangement includes
USB functional modules, circuit arrangement pass through USB functional modules or bluetooth connection terminal.
7. the high quick fluorescent chromatographic device of group of the lanthanides according to claim 1 or 6, it is characterised in that：The terminal connection interconnection
Net, terminal through internet obtains the standard curve of detection sample, and testing result storage is arrived into internet.
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| CN201720742591.9U CN206945538U (en) | 2017-06-24 | 2017-06-24 | The high quick fluorescent chromatographic device of group of the lanthanides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037025A (en) * | 2017-06-24 | 2017-08-11 | 杭州微瑞科技有限公司 | The high quick fluorescent chromatographic device of group of the lanthanides and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037025A (en) * | 2017-06-24 | 2017-08-11 | 杭州微瑞科技有限公司 | The high quick fluorescent chromatographic device of group of the lanthanides and detection method |
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