CN102539366A - Device for detecting swinging bed fermentation liquid by double light sources - Google Patents
Device for detecting swinging bed fermentation liquid by double light sources Download PDFInfo
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- CN102539366A CN102539366A CN2011104491827A CN201110449182A CN102539366A CN 102539366 A CN102539366 A CN 102539366A CN 2011104491827 A CN2011104491827 A CN 2011104491827A CN 201110449182 A CN201110449182 A CN 201110449182A CN 102539366 A CN102539366 A CN 102539366A
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Abstract
The invention provides a device for detecting swinging bed fermentation liquid by double light sources. The device comprises swinging bed equipment, a liquid fermentation triangular bottle and a glass triangular bottle, wherein the liquid fermentation triangular bottle is arranged on a swinging bed, the glass triangular bottle contains fermentation liquid, is coated with a black coating and is fixedly arranged on a silica gel frame, the silica gel frame is fixedly arranged on a swinging bed tray, the inner side of the silica gel frame is provided with two light emitting electrodes and corresponding light receiving electrodes, the two light emitting electrodes mutually form an angle being 90 degrees, in addition, the light emitting electrodes and the light receiving electrodes are anastomotic with the positions of light transmission holes arranged on the triangular bottle containing fermentation liquid in the middle, simultaneously, the inner side of the silica gel frame at the other end is also provided with two light emitting electrodes and corresponding light receiving electrodes, the two light emitting electrodes mutually form an angle being 90 degrees, in addition, the light emitting electrodes and the light receiving electrodes are anastomotic with the other two through holes arranged on the triangular bottle containing the fermentation liquid in the middle, received light signals are converted into electric signals by the light receiving electrodes, the fermentation liquid concentration is increased along with the gradual increase of the biological thallus quantity in the triangular body, the transmitted light is changed, and the electric signals of the light receiving electrodes are also changed, so the growth degree of the fermentation thalli in the triangular bottle is judged. Through the double light sources, the detection sensitivity can be improved, and simultaneously, the device is also suitable for being used for detecting the work states of various kinds of fermentation liquid in industry.
Description
Technical field
The present invention relates to a kind of device of tracer liquid sweat.
Technical background
Detect at present the fermented liquid on the shaking table, all be to see through glass triangle bottle range estimation Direct observation or take out fermented liquid and carry out the thalline counting, or filter centrifugal; Take by weighing the weight of solid content, confirm means such as biomass, analytic process is complicated; Time is long, and error is bigger.
Content of the present invention
Analyze the problem that exists in order to solve above-mentioned fermentation; The present invention provides the device of a kind of pair of light source detection shaker fermentation liquid; Comprise shaking table equipment, place the liquid fermentation triangular flask on the shaking table, fill fermented liquid and scribble the glass triangle bottle of black coating; Be fixed on the silica gel frame; The silica gel frame is fixed on the shaking table pallet, and silica gel frame inboard is provided with two light emitting electrodes that are in an angle of 90 degrees and corresponding light-receiving electrodes, and matches with the light hole position that is equipped with on the triangular flask of fermented liquid the centre; Simultaneously other end silica gel frame inboard also is provided with two light emitting electrodes and corresponding light-receiving electrodes that are in an angle of 90 degrees, and matches with two open-works in addition that are equipped with on the triangular flask of fermented liquid the centre; The light-receiving electrode converts the light signal that receives into electric signal; Increase gradually with the biological bacteria scale of construction in the triangular flask, fermented liquid concentration increases, and the light that sees through is changed; The electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in the triangular flask.
Said triangular flask, except being mutually four light holes of an angle of 90 degrees, and outside the triangular flask bottom, all the other all scribble the black light screening material, and light hole is all below the fermentation liquid level.
Said two ultraviolet rays that transmitting illuminant is infrared ray or selected wavelength, two wavelength or different wave lengths that emission is only identical.
Said two light-receiving electrodes are connected with photoelectric sensor, convert the light signal that receives into electric signal, through being wirelessly transmitted to display screen, or mobile phone, or on the media player, the extent of growth of fermentation liquor thalline in the instant playback shaking table triangular flask.
Advantage of the present invention is: 1) adopt photoelectricity as detection means, the photoelectricity propagation distance is far away, can reach several km, and it is minimum to be used for bottle interior very-short-reach propagated error.2) two light sources can detect two samples in the fermented liquid simultaneously, can distinguish that like infrared light solution and colloid, ultraviolet light wave can confirm the group molecule, and thalline increases can the change strength of fluid; Light absorption value there is direct influence; Therefore two light sources are surveyed fermented liquid, and are highly sensitive, are prone to apply.3) utilize the solid neutral colour filter to obtain light source, method is simple, economy and durability, and technology maturation is easy to realize suitability for industrialized production.4) compare with the traditional glass Erlenmeyer flask, adopt the shading triangular flask, many advantages are arranged: at first the black coating light-proofness is good, can avoid diffracted ray to disturb, and guarantees the photoelectricity operating accuracy; Secondly, chrome-plated material shading triangular flask conduction is good, and the temperature that can effectively utilize shaking table to provide also provides advantage for exploitation bottom alternating temperature shaking table pallet; 5) the silica stationary frame is flexible, and is sturdy and durable, prevents the triangular flask displacement and comes off, and can also place light emission and light absorption electric elements in inside.6) device of two light source detection shaker fermentation liquid can be drawn the photoelectric absorption curve through revision test, and according to the curve programming, the Based Intelligent Control shaking table repeats to ferment each stage, operates production routine accurately for industrial bulk fermentation provides.7) need not take a sample detect can through display screen and (or) mobile phone; (or) multimedia etc. shows fermentation liquor terms and conditions and relevant information; The design of shaker fermentation orthogonal experiment particularly can be provided, accurate experiment parameter is provided, improve scientific research efficient and experimental precision.
Description of drawings
Fig. 1 is the two light source detection shaker fermentation liquid mounting structure figure of the present invention;
The two light source triangular flask structural drawing of Fig. 2.
Code name explanation among the figure
1, triangle shakes bottle; 2, light hole;
3, silica gel frame; 4,7, emitting electrode;
5,9 receiving electrodes (9 4 the back side); 8,10 photoelectric sensors (10 4 the back side);
6, shaking table pallet, 11, the shading coating; 12,13,14,15 light holes (15 14 the back side).
Embodiment
The present invention includes shaking table equipment; Place the liquid fermentation triangular flask 1 on the shaking table; Fill fermented liquid and scribble the glass triangle bottle of black coating, be fixed on the silica gel frame 3, silica gel frame 3 is fixed on the shaking table pallet 6; Silica gel frame 3 inboards are provided with two the light emitting electrodes 4 and light-receiving electrode 5 that are in an angle of 90 degrees; And match with light hole 14,12 positions that are equipped with on the triangular flask 1 of fermented liquid the centre, other end silica gel frame 3 inboards also are provided with two light emitting electrodes 7 that are in an angle of 90 degrees and light-receiving electrode 9 (9 4 the back side) simultaneously, and match with two open-works 13,15 in addition that are equipped with on the triangular flask of fermented liquid the centre; Light-receiving electrode 5,9 converts the light signal that receives into electric signal; Increase gradually with the biological bacteria scale of construction in the triangular flask, fermented liquid concentration increases, and the light that sees through is changed; The electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in the triangular flask.
Said triangular flask, except being mutually four light holes 12,13,14,15 of an angle of 90 degrees, and outside the triangular flask bottom, all the other all scribble black light screening material 11, and light hole is all below the fermentation liquid level.
Said two ultraviolet rays that transmitting illuminant is infrared ray or selected wavelength, two wavelength or different wave lengths that emission is only identical.
Said two light-receiving electrodes 5,9 are connected with photoelectric sensor 8,10, convert the light signal that receives into electric signal, through being wirelessly transmitted to display screen, or mobile phone, or on the media player, the extent of growth of fermentation liquor thalline in the instant playback shaking table triangular flask.
Embodiment 1:
Bacterial classification: get three kinds of different lactic acid bacteria culturers, L
1, L
2And L
3, processing bacteria containing amount respectively is 10
5 ~ 6Individual/ml suspension bacteria liquid is subsequent use;
Sterilization: get 250ml shading triangular flask, respectively add beef broth potato nutrient solution 100ml＜± 0.1% (getting 10% beef broth nutrient solution 20ml adds in the PDA potato nutrient solution) after the numbering, sterilization cooling back is subsequent use for the nutrition fermented liquid;
Inoculation:Measure the bacteria suspension 5ml of different strain with pipettor, add and contain in the shading triangular flask of nutritious fermented liquid, more than each bacterial classification adorn three bottles (they being triplicate), only a fermented liquid triangular flask does not add bacterial classification, is used for contrast, more than totally 10 bottles;
Install: the shading triangular flask is fixed on the silica gel frame, and the turn-knob of screwing matches optoelectronic pole and triangular flask light hole.Open photoelectricity power switch on the control panel, make on the silica gel frame light of two light source luminescent electrodes, see through the triangular flask nutrient liquid, receive to the light-receiving electrode of opposite side;
The start operation: open shaking table control panel photoelectricity master switch,, the light absorption number of all triangular flasks is put be zero through fine setting; Adjustment shaking table temperature is 32 ℃, and revolution is 250 rev/mins, starts shaking table;
Observe: every measured an absorbance value at a distance from 30 minutes, after the peak appears in absorbance value when on a declining curve only.Relatively more two light source gained results are horizontal ordinate with time, are ordinate with the absorbance value, draw absorption curves, are the best fermentation time of this mushroom type with the light absorption peak value when the highest; With light absorption peak value the maximum, be the dominant strain of the best.More than can be the basic operation standard of Intelligent Measurement shaker fermentation liquid bio quality.
Embodiment 2:
Get the advantage lactic acid bacteria strains among the embodiment 1, processing bacteria containing amount is 10
5 ~ 6Individual/ml suspension bacteria liquid is subsequent use; Sterilization, inoculation, installation and start operation are with embodiment 1; Wherein two optical source wavelengths 1 are set at infrared light, detect the fermented liquid colloid concentration; Wavelength 2 is set at ultraviolet light, detects the glucose residual volume in the fermented liquid, and initial light absorption value is made as zero, and with the growth of fermentation thalline, glucose content descends, and wavelength 2 light absorption values can present negative value; Whenever at a distance from absorbance of detection in 30 minutes, sample analysis thalline content, the residual amount of glucose,, suitably add nutrient solution and adjustment ph after the shaking table work according to analysis result; Data storage; Repeated experiments is three times continuously, average, and with time horizontal ordinate; With absorbance, thalline content and the residual amount of glucose is that ordinate is figure, draws the two light source light electro-detection shaking table working curves of this bacterial strain.The working curve method of application is: by the nutrient formulation that this shaking table provides, bottle, sterilization, cooling inoculation, Installation and Debugging, set wavelength 1 and wavelength 2; Start shooting etc., absorbance value and the pairing typical curve result of absorbance value in the close observation fermentation time, close in value shows that then fermentation system is in proper working order; And press the typical curve operation indicating; In time add nutrient solution and adjustment ph,, finally obtain desirable fermentation result until end; If online numerical value and typical curve differ greatly, can report to the police and point out possibly occur unusual automatically, shutdown inspection is avoided loss in case of necessity.
Two electrodes of two light source light electro-detection shaking tables can send the light of identical wavelength or different wave length, help detecting simultaneously detected materials two or more in the fermentation liquor, like the colloid concentration in the fermented liquid or certain biopreparate; With the yeast culture time lengthening, changing appears in bacterial concentration, and bacterial concentration is high, shows that thalline content is many, is directly proportional with the absorbance increase; Through observing the variation of two light source absorbances, help analyzing more accurately, faster fermentation condition to the influence of fermented liquid thalline activity and the product quality of definite fermented liquid, the detection of no longer need taking a sample.Two light source light electro-detection shaker fermentation liquid, used triangular flask are except that adopting the black shading to film and have the sensitization position, and other and common triangular flask are identical; The course of work of two light source detection shaker fermentation liquid is: 1) prepare liquid spawn: according to the different experiments needs, prepare liquid spawn, the preparation suspension bacteria liquid is subsequent use; 2) preparation nutrition fermented liquid:, accurately prepare the nutrient matrix of suitable growth according to the different strain growth needs; When using 250ml shading triangular flask, add nutrient liquid 100 with (or) 120ml, loading amount error＜± 0.1%; 3) sterilization: the shading triangular flask of being added with nutrient liquid is carried out the high-temperature steam sterilization, and in 121 ℃, pressure 0.15Mpa kept 15 minutes, can insert bacterial classification when being cooled to 23 ~ 26 ℃; 4) inoculation: bacterium liquid suspension concentration is controlled at 10
6 ~ 7Individual/ml, with pipettor accurately add 5 with (or) 10ml bacterium liquid/every bottle; When inoculating thread thalline, use 0.5cm
2Card punch get mycoderm 5 with (or) 10 slices, insert and contain in the shading triangular flask of nutritious fermented liquid; During inoculation wherein the nutrient liquid of at least one triangular flask do not add bacterial classification, be contrast; 5) start debugging: open general supply, the adjustment electroluminescent lamp, the light source that electroluminescent lamp is sent sees through the triangular flask nutrient liquid, is received by triangular flask opposite side light-receiving electrode, changes electric signal into, handles obtaining tangible numerical signal again through the multiplication amplifying circuit; 6) wavelength set of two optoelectronic poles: set the light that two optoelectronic poles send identical wavelength or different wave length according to different test sample needs; As measure the remaining concentration of concentration and glucose of thalline in the fermented liquid simultaneously, can set an electrode light source respectively and send the 100nm infrared wavelength and send the 260nm ultraviolet wavelength with another; For satisfying L
9(3)
4Orthogonal test needs, and is one group with 10 usually, can detect three different samples, and wherein 9 are inserted bacterial classification, and 1 does not connect bacterial classification as contrast.Usually triplicate is averaged and is done regretional analysis.The silica stationary frame is anchored on the shaking table pallet, and light emitting electrode, light absorption photoelectric commutator place in the silica gel frame, with wireless transmit, through display screen and (or) mobile phone and (or) demonstration such as multimedia.Two light source detection shaking table liquid fermentation principle of work: fermented liquid will be housed and insert the shading triangular flask of bacterial classification, and be fixed on the shaking table silica gel frame, the frame turn-knob that is tightened makes two light emitting electrodes identical automatically with two light-receiving electrodes and four light holes of triangular flask; Open optoelectronic switch and make two emitting electrodes all luminous, and send specific light wave, see through nutrient liquid until the triangular flask opposite side through optical filter; Received by two light-receiving electrodes, initial through the fine setting turn-knob, photoelectricity is received the display number put that all to transfer to be zero; Start shaking table after setting revolution (150 ~ 160 rev/mins), temperature (25 ~ 28 ℃), with shaking table work time lengthening, fermented liquid change in concentration; Changing appears in numerical value on the photoelectricity receptor, when numerical value is constant till, at this moment numerical value is big; Thalline content is maximum in the corresponding triangular flask, the also significant change of biochemical metabolism index, and contrast triangular flask numerical value still is zero.Thus, can confirm the product quality of shaker fermentation liquid.
Claims (4)
1. the device of two light source detection shaker fermentation liquid; Comprise shaking table equipment; Place the liquid fermentation triangular flask on the shaking table; It is characterized in that: fill fermented liquid and scribble the glass triangle bottle of black coating, be fixed on the silica gel frame, the silica gel frame is fixed on the shaking table pallet; Silica gel frame inboard is provided with two the light emitting electrodes and corresponding light-receiving electrode that are in an angle of 90 degrees; And match with the light hole position that is equipped with on the triangular flask of fermented liquid the centre, other end silica gel frame inboard also is provided with two light emitting electrodes and corresponding light-receiving electrodes that are in an angle of 90 degrees simultaneously, and matches with two light holes in addition that are equipped with on the triangular flask of fermented liquid the centre; The light-receiving electrode converts the light signal that receives into electric signal; Increase gradually with the biological bacteria scale of construction in the triangular flask, fermented liquid concentration increases, and the light that sees through is changed; The electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in the triangular flask.
2. the device of according to claim 1 pair of light source detection shaker fermentation liquid is characterized in that, said triangular flask; Except being mutually four light holes of an angle of 90 degrees; And outside the triangular flask bottom, all the other all scribble the black light screening material, and light hole is all below the fermentation liquid level.
3. the device of according to claim 1 pair of light source detection shaker fermentation liquid is characterized in that, said two ultraviolet rays that transmitting illuminant is infrared ray or selected wavelength, two wavelength or different wave lengths that emission is only identical.
4. the device of according to claim 1 pair of light source detection shaker fermentation liquid; It is characterized in that said two light-receiving electrodes are connected with photoelectric sensor, convert the light signal that receives into electric signal; Through being wirelessly transmitted to display screen; Or mobile phone, or on the media player, the extent of growth of fermentation liquor thalline in the instant playback shaking table triangular flask.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110449182 CN102539366B (en) | 2011-12-29 | 2011-12-29 | Device for detecting swinging bed fermentation liquid by double light sources |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110449182 CN102539366B (en) | 2011-12-29 | 2011-12-29 | Device for detecting swinging bed fermentation liquid by double light sources |
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| CN102539366A true CN102539366A (en) | 2012-07-04 |
| CN102539366B CN102539366B (en) | 2013-12-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323433A (en) * | 2013-06-20 | 2013-09-25 | 上海智城分析仪器制造有限公司 | Shaking bed device of non-contact-type multichannel online detection fermentation liquid as well as testing method |
| CN115436432A (en) * | 2022-09-01 | 2022-12-06 | 东莞市振海电子科技有限公司 | Method for monitoring concentration of liquid in pipe |
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| CN202030764U (en) * | 2011-05-06 | 2011-11-09 | 安徽丰原生物化学股份有限公司 | Fermentation bottle and bio-fermentation culture device provided with same |
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2011
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| US5396075A (en) * | 1993-07-27 | 1995-03-07 | The United States Of America As Represented By The United States Department Of Energy | Method for in situ characterization of a medium of dispersed matter in a continuous phase |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323433A (en) * | 2013-06-20 | 2013-09-25 | 上海智城分析仪器制造有限公司 | Shaking bed device of non-contact-type multichannel online detection fermentation liquid as well as testing method |
| CN103323433B (en) * | 2013-06-20 | 2016-08-10 | 上海智城分析仪器制造有限公司 | The shaking table device of contactless multichannel on-line checking fermentation liquid and method of testing |
| CN115436432A (en) * | 2022-09-01 | 2022-12-06 | 东莞市振海电子科技有限公司 | Method for monitoring concentration of liquid in pipe |
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| CN102539366B (en) | 2013-12-18 |
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