CN102445439A - Chemiluminescence detection device - Google Patents
Chemiluminescence detection device Download PDFInfo
- Publication number
- CN102445439A CN102445439A CN2010105008938A CN201010500893A CN102445439A CN 102445439 A CN102445439 A CN 102445439A CN 2010105008938 A CN2010105008938 A CN 2010105008938A CN 201010500893 A CN201010500893 A CN 201010500893A CN 102445439 A CN102445439 A CN 102445439A
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- CN
- China
- Prior art keywords
- test board
- hole
- revolving part
- chemiluminescence detector
- catoptron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002038 chemiluminescence detection Methods 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000004806 packaging method and process Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 description 5
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 2
- 108060001084 Luciferase Proteins 0.000 description 2
- 239000005089 Luciferase Substances 0.000 description 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
Images
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a chemiluminescence detection device which comprises a test desk, wherein the test desk is internally provided with an accommodating chamber, a groove is formed on the upper surface of the test desk and communicated with the accommodating chamber, a rotating member is arranged in the accommodating chamber and can rotate in the accommodating chamber, the rotating member is provided with a through hole which can accommodate a to-be-detected sample through the groove, a reflecting mirror is arranged at the bottom of the test desk and used for reflecting fluorescent light of the to-be-detected sample, and the reflecting mirror is located under the through hole when the rotating member rotates to a fixed position. The chemiluminescence detection device further comprises a light detection device which is arranged on the test desk and receives the reflected light and fluorescent light through the through hole, a signal processing device which calculates the quantity of fluorescent light emitted from the to-be-detected sample according to the output signal from the light detection device, and a display device for displaying the quantity of fluorescent light. The test desk, the light detection device and the signal processing device are packaged in a packaging box; and the display device is arranged on the outer surface of the packaging box. The chemiluminescence detection device is high in sensitivity and high in signal-to-noise ratio, and can be conveniently used in on-site detection.
Description
Technical field
The present invention relates to a kind of detector, relate in particular to a kind of chemiluminescence detector.
Background technology
Along with the success of genetic engineering modified luciferase, it is day by day ripe that chemiluminescence is surveyed biological information.Its major advantage is that signal to noise ratio (S/N ratio) is high, good stability.Can be widely used in the potable water monitoring in biological study, medical diagnosis, food security, anti-terrorism, customs inspection and calamity period etc.Luciferase is running into target substrates generation photon, and concentration of substrate is high more, and the photon of generation is many more, just can judge the concentration of target substrates indirectly through surveying light intensity.For example, this method some one-tenth that can be used to detect in the saliva assigns to judge whether the amusement practitioner takes drugs.Classic method often need be delivered to specialized laboratories with saliva sample and detect, reason be existing instrument volume big, can not be portable and cost high, entirely for import, can't popularize.And existing instrument detecting accuracy, sensitivity and signal to noise ratio (S/N ratio) remain further to be improved.
Summary of the invention
In view of the problems referred to above that exist in the prior art, fundamental purpose of the present invention is to address the deficiencies of the prior art, and a kind of portable, highly sensitive, chemiluminescence detector that cost is low is provided.
A kind of chemiluminescence detector; Comprise a test board; Has a host cavity in the said test board; Also offer the groove that is connected with said host cavity along the upper surface of said test board; Have the revolving part that can in said host cavity, rotate in the said host cavity, said revolving part is provided with one and accommodates the through hole of a detected sample through said groove, and the bottom of said test board has the catoptron of a reflection fluorescence that detected sample sends; Said catoptron when said revolving part rotates to a fixed position, be positioned at said through hole under, said chemiluminescence detector comprises that also one is installed on the said test board and can receives the reflected light of said catoptron and the light detection device of fluorescence that detected sample sends through said through hole.
A kind of chemiluminescence detector; Comprise a test board; Has a host cavity in the said test board; Also offer the groove that is connected with said host cavity along the upper surface of said test board; Has the revolving part that can in said host cavity, rotate in the said host cavity; Said revolving part is provided with one and accommodates the through hole of a detected sample through said groove; The bottom of said test board has the catoptron of a reflection fluorescence that detected sample sends, said catoptron when said revolving part rotates to a fixed position, be positioned at said through hole under, said chemiluminescence detector comprises that also one is installed on the said test board and can receives through said through hole the display device of quantity of signal processing apparatus and the said fluorescence of a demonstration of the quantity of the fluorescence that the light detection device, of the reflected light of said catoptron and fluorescence that detected sample sends sends according to the said detected sample of output calculated signals of said light detection device; Said test board, light detection device and signal processing apparatus are encapsulated in the package, and said display device is located on the outside surface of said package.
Beneficial effect of the present invention is: said chemiluminescence detector utilizes catoptron, makes said photomultiplier detect double fluorescence, thereby has improved the sensitivity and the signal to noise ratio (S/N ratio) of fluoroscopic examination.Said chemiluminescence detector is packaged together test board, catoptron, photomultiplier, signal processing apparatus, display device, has portability, and volume is little, and cost is low, can break away from special laboratory and carry out the scene detection.
Description of drawings
Fig. 1 is the structural representation of chemiluminescence detector preferred embodiments of the present invention.
Fig. 2 is the block diagram of chemiluminescence detector among Fig. 1.
Fig. 3 is test board and the structural representation of photomultiplier among Fig. 2.
Fig. 4 is the structural representation of revolving part among Fig. 3.
Fig. 5 is the structural representation of Fig. 3 test board lower cover.
Embodiment
To combine accompanying drawing and specific embodiment that the present invention is described further below.
Please refer to Fig. 1 to Fig. 3; Chemiluminescence detector of the present invention comprises a package 10, is equiped with a test board 20, in the packaged case 10 and is installed in a light detection device and a signal processing apparatus 40 on the said test board, and the upper surface of said package 10 also has a display device 50; The interface 60 that has a connection outer computer on the said package 10; Said interface 60 is a USB interface, and in this embodiment, said light detection device is a photomultiplier 30.
Please continue with reference to figure 4 and Fig. 5; Said test board 20 comprises a loam cake 22 and a lower cover 24 that is fixed together; Has a host cavity between said loam cake 22 and the lower cover 24; Offer a mounting groove 28 on the lower cover 24 of said test board 20, be equiped with a catoptron in the said mounting groove 28, said catoptron be positioned at said photomultiplier 30 under.Be horizontally installed with a revolving part 26 in the said host cavity; Cephalolateral margin along said loam cake 22 and lower cover 24 respectively offers a breach; To form the opening that is connected with said host cavity; A part of lateral margin of said revolving part 26 passes said opening, and exposes outside the said package 10, in this embodiment; Said revolving part 26 is cylindric, through stir part that said revolving part 26 exposes said package 10 can make said revolving part 26 in said host cavity around the rotation of the center of said revolving part 26.
Upper surface along said revolving part 26 offers a through hole 262; Said through hole 262 is used to hold sample to be detected; Along the upper surface of said test board 20, the upper surface of promptly said loam cake 22 also offers the groove 27 that is connected with said host cavity, when said revolving part 26 rotates to a primary importance; Said groove 27 is connected with through hole 262; When said revolving part 26 rotated to a second place, said through hole 262 was connected with said mounting groove 28, said catoptron be positioned at said through hole 262 under.
Also be provided with at least one flexible positioning piece 263 on the said revolving part 26; Each said flexible positioning piece 263 comprises that one is embedded at the ball that spring and in the said revolving part 26 is located at said spring one end; Lower surface at said loam cake 22 offers a plurality of pilot holes that adapt with ball size each flexible positioning piece 263; When said revolving part 26 rotates to said first or during the second place; The ball of each flexible positioning piece 263 is replaced the pilot hole in correspondence, and sends mechanical collision sound and put in place to remind operator's revolving part 26 to rotate, operator's said revolving part that just can stop the rotation.
When a sample is carried out fluoroscopic examination; Can make said revolving part 22 around its center rotation through stirring the part that said revolving part 26 exposes said package 10; Make said revolving part 26 rotate to said primary importance; Sample to be detected is placed in the said through hole 262 through said groove 27, makes it be in a second place through rotating said revolving part 26 again, the detected sample in the through hole 262 is in the top of said catoptron at this moment.In this embodiment, can said through hole 262 be designed to the through hole of an aperture convergent, drop on said lower cover 24 to prevent said detected sample.
The fluorescence that said detected sample sends forms the reflected light identical with said amount of fluorescence through said catoptron 70 reflection backs; Fluorescence that said detected sample sends and said reflected light are through the said photomultiplier 30 of said through hole directive; Said photomultiplier 30 is surveyed fluorescence and the said reflected light that said detected sample sends simultaneously; And the photon that detects is converted into current signal is sent to said signal processing apparatus 40; Said signal processing apparatus 40 calculates the quantity of the fluorescence that said detected sample sends according to the current signal that receives; And according to the amount of fluorescence that calculates, use preset algorithm and calculate the luciferin concentration of substrate in the detected sample, said signal processing apparatus 40 is sent to said display device 50 with the concentration of the quantity of the fluorescence that calculates and luciferin substrate and shows.
Said signal processing apparatus 40 also can be sent to the data that calculate that outer computer stores or further analyzes through said interface 60.
Said chemiluminescence detector utilizes catoptron; Make said photomultiplier detect double fluorescence; Thereby improved the sensitivity and the signal to noise ratio (S/N ratio) of fluoroscopic examination, said chemiluminescence detector is packaged together test board, catoptron, photomultiplier, signal processing apparatus, display device, can provide work required electric energy through reserve battery or vehicle power; Can break away from special laboratory and carry out the scene detection, easy to use.
Claims (10)
1. chemiluminescence detector; It is characterized in that; Said chemiluminescence detector comprises a test board; Have a host cavity in the said test board, also offer the groove that is connected with said host cavity, have the revolving part that can in said host cavity, rotate in the said host cavity along the upper surface of said test board; Said revolving part is provided with one and accommodates the through hole of a detected sample through said groove; The bottom of said test board has the catoptron of a reflection fluorescence that detected sample sends, said catoptron when said revolving part rotates to a fixed position, be positioned at said through hole under, said chemiluminescence detector comprises that also one is installed on the said test board and can receives the reflected light of said catoptron and the light detection device of fluorescence that detected sample sends through said through hole.
2. chemiluminescence detector as claimed in claim 1; It is characterized in that; Also comprise at least one pilot hole in the said test board; Said revolving part is provided with at least one flexible positioning piece, and said at least one flexible positioning piece comprises that one is embedded at the spring on the said revolving part and and is installed in an end of said spring and can corresponding replaces the ball in said at least one pilot hole.
3. chemiluminescence detector as claimed in claim 1 is characterized in that, said light detection device is a photomultiplier.
4. chemiluminescence detector as claimed in claim 1; It is characterized in that said test board comprises a loam cake and a lower cover that interfixes, said groove is opened in and covers on said; Cover under said and offer a mounting groove, said catoptron is installed in the said mounting groove.
5. chemiluminescence detector as claimed in claim 1 is characterized in that, said through hole is the through hole of an aperture convergent, and the part lateral margin of said revolving part exposes outside the said test board.
6. chemiluminescence detector; It is characterized in that; Said chemiluminescence detector comprises a test board; Has a host cavity in the said test board; Also offer the groove that is connected with said host cavity along the upper surface of said test board; Have the revolving part that can in said host cavity, rotate in the said host cavity, said revolving part is provided with one and accommodates the through hole of a detected sample through said groove, and the bottom of said test board has the catoptron of a reflection fluorescence that detected sample sends; Said catoptron when said revolving part rotates to a fixed position, be positioned at said through hole under; Said chemiluminescence detector comprises that also one is installed on the said test board and can shows through the signal processing apparatus and that said through hole receives the quantity of the fluorescence that the light detection device, of the reflected light of said catoptron and fluorescence that detected sample sends sends according to the said detected sample of output calculated signals of said light detection device the display device of the quantity of said fluorescence, and said test board, light detection device and signal processing apparatus are encapsulated in the package, and said display device is located on the outside surface of said package.
7. chemiluminescence detector as claimed in claim 6 is characterized in that, said through hole is the through hole of an aperture convergent, and said revolving part is cylindrical, and the part lateral margin of said revolving part exposes outside the said package.
8. chemiluminescence detector as claimed in claim 6; It is characterized in that said test board comprises a loam cake and a lower cover that interfixes, said groove is opened in and covers on said; Cover under said and offer a mounting groove, said catoptron is installed in the said mounting groove.
9. chemiluminescence detector as claimed in claim 8; It is characterized in that; Said loam cake or under cover and be provided with at least one pilot hole; Said revolving part is provided with at least one flexible positioning piece, and said at least one flexible positioning piece comprises that one is embedded at the spring on the said revolving part and and is installed in an end of said spring and can corresponding replaces the ball in said at least one pilot hole.
10. chemiluminescence detector as claimed in claim 6 is characterized in that, has the interface of a connection outer computer on the said package.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010500893.8A CN102445439B (en) | 2010-10-09 | 2010-10-09 | chemical fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010500893.8A CN102445439B (en) | 2010-10-09 | 2010-10-09 | chemical fluorescence detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102445439A true CN102445439A (en) | 2012-05-09 |
| CN102445439B CN102445439B (en) | 2015-09-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010500893.8A Active CN102445439B (en) | 2010-10-09 | 2010-10-09 | chemical fluorescence detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102445439B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278484A (en) * | 2013-05-11 | 2013-09-04 | 徐培实 | Portable fluorescent brightener detector |
| CN109781680A (en) * | 2018-12-27 | 2019-05-21 | 吉林省农业科学院 | A kind of fluorescence detection device |
| CN110618113A (en) * | 2019-08-09 | 2019-12-27 | 苏州百源基因技术有限公司 | Multicolor fluorescence reader |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6947136B2 (en) * | 1999-01-25 | 2005-09-20 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| CN101086478A (en) * | 2006-06-06 | 2007-12-12 | 同济大学 | Fluorescent quantitative detection device for PCR |
| CN101782519A (en) * | 2009-01-15 | 2010-07-21 | 陈翰民 | Device for exciting fluorescent sample by utilizing visible light |
| CN201553741U (en) * | 2009-11-16 | 2010-08-18 | 杭州博日科技有限公司 | Multiwavelength fluorescence detection device of quantitative PCR |
| CN201828520U (en) * | 2010-10-09 | 2011-05-11 | 北京网新易尚科技有限公司 | Chemical fluorescence detector |
-
2010
- 2010-10-09 CN CN201010500893.8A patent/CN102445439B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6947136B2 (en) * | 1999-01-25 | 2005-09-20 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| CN101086478A (en) * | 2006-06-06 | 2007-12-12 | 同济大学 | Fluorescent quantitative detection device for PCR |
| CN101782519A (en) * | 2009-01-15 | 2010-07-21 | 陈翰民 | Device for exciting fluorescent sample by utilizing visible light |
| CN201553741U (en) * | 2009-11-16 | 2010-08-18 | 杭州博日科技有限公司 | Multiwavelength fluorescence detection device of quantitative PCR |
| CN201828520U (en) * | 2010-10-09 | 2011-05-11 | 北京网新易尚科技有限公司 | Chemical fluorescence detector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278484A (en) * | 2013-05-11 | 2013-09-04 | 徐培实 | Portable fluorescent brightener detector |
| CN109781680A (en) * | 2018-12-27 | 2019-05-21 | 吉林省农业科学院 | A kind of fluorescence detection device |
| CN110618113A (en) * | 2019-08-09 | 2019-12-27 | 苏州百源基因技术有限公司 | Multicolor fluorescence reader |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102445439B (en) | 2015-09-02 |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 100081, Beijing, Zhongguancun, Haidian District South Avenue, No. 18, Beijing international A block 4 Applicant after: YISHANG TOMORROW TECHNOLOGY Co.,Ltd. Address before: 100081, Beijing, Zhongguancun, Haidian District South Avenue, No. 18, Beijing international A block 4 Applicant before: Beijing Wangxin Yishang Technology Co.,Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: BEIJING INNOVATION TECH. CO., LTD. TO: YISHANG TOMORROW TECHNOLOGY CO., LTD. |
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