CN109781680A - A kind of fluorescence detection device - Google Patents
A kind of fluorescence detection device Download PDFInfo
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- CN109781680A CN109781680A CN201811611863.7A CN201811611863A CN109781680A CN 109781680 A CN109781680 A CN 109781680A CN 201811611863 A CN201811611863 A CN 201811611863A CN 109781680 A CN109781680 A CN 109781680A
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- detection device
- fluorescence detection
- supporting body
- pedestal
- optical
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Abstract
The present invention provides a kind of fluorescence detection devices, are related to detection device technical field, solve the fluorescence detection device existing in the prior art technical problem that structure is complicated, detection effect is bad.The device includes outer housing, sample placing modules and optical de-tection means is equipped in the outer housing, wherein the bottom of the sample placing modules forms a fixed connection with the outer housing；The optical de-tection means are connect to receive the fluorescent of the sample placing modules and be translated into electric signal with the sample placing modules.Fluorescence detection device structure of the invention is simple, can detect simultaneously to multiple samples to be tested, while improving the transmission efficiency of exciting light and fluorescence, reduce optical path loss, improve the utilization rate of light, improve detection effect.
Description
Technical field
The present invention relates to detection device technical fields, more particularly, to a kind of fluorescence detection device.
Background technique
Fluorescence detection device is the common detector of high pressure liquid chromatography, chromatographic fraction is irradiated with ultraviolet light, when sample group
When dividing with fluorescence property, it can be detected.With the success of genetic engineering transformation luciferase, chemiluminescence detection biology letter
Breath is increasingly mature.In corresponding chemical reaction, the generation of fluorescence comes from the oxidation of fluorescein.By luciferase and
ATP is reacted, and can detecte human body cell, bacterium, mould, swill.Luciferase can when encountering target substrates
Photon is generated, concentration of substrate is higher, and the photon of generation is more, can judge the dense of target substrates indirectly by detecting luminous intensity
Degree.
Applicants have discovered that fluorescence detection device in the prior art at least has a disadvantage that existing fluorescence detection equipment
Volume is larger, and structure is complicated, not readily portable, and cost is high, can not popularize.Meanwhile the optics of existing fluorescence detection device
System, there are potential fluorescence crosstalks, cause detection error larger, Detection accuracy decline.
Summary of the invention
The purpose of the present invention is to provide a kind of fluorescence detection devices, to solve fluorescence detection dress existing in the prior art
Set the technical problem that structure is complicated, detection effect is bad.Optimal technical scheme institute in many technical solutions provided by the invention
The many technical effects elaboration as detailed below that can be generated.
To achieve the above object, the present invention provides following technical schemes:
A kind of fluorescence detection device provided by the invention, including outer housing are equipped with sample placement group in the outer housing
Part and optical de-tection means, wherein the bottom of the sample placing modules forms a fixed connection with the outer housing；The optics
Testing agency is connect to receive the fluorescent of the sample placing modules and be translated into electricity with the sample placing modules
Signal.
A preferred embodiment of the invention, the sample placing modules include pedestal and are arranged in the pedestal
It can be equipped with above and in the pedestal accommodating chamber for accommodating the supporting body relative to the supporting body of the base rotation,
The upper end of the accommodating chamber is open-type structure.
A preferred embodiment of the invention is equipped with opens with what the accommodating chamber was connected on the base
Mouthful, the opening is for making the supporting body be put into or take out in the accommodating chamber.
A preferred embodiment of the invention is equipped with annular slide track, described on the bottom surface of the accommodating chamber
The bottom of supporting body is equipped with sliding block, and the sliding block can be connected on the slideway, so that the supporting body passes through the sliding block
It is rotated relative to the slideway.
A preferred embodiment of the invention is symmetrically equipped with multiple putting holes on the top of the supporting body,
Wherein, the putting hole is centrosymmetric setting about the center of the supporting body.
A preferred embodiment of the invention is additionally provided with top cover, the top cover and institute on the top of the base
It states and forms a fixed connection above the accommodating chamber of pedestal.
A preferred embodiment of the invention, being equipped on the top cover about the center of the top cover is in center
Symmetrical at least two peep hole, the size of the peep hole are consistent with the size of the putting hole.
The top of the top cover is arranged in a preferred embodiment of the invention, the optical de-tection means, and
And the fixing piece of the optical de-tection means is arranged above at least one described peep hole, the fixing piece is that cylinder is hollow
Type structure.
A preferred embodiment of the invention, in the optical de-tection means and be located at the fixing piece just on
Side is provided tiltedly with dichroscope, parallel with the dichroscope to be equipped with reflecting mirror, wherein the dichroscope and described anti-
Mirror inclination is penetrated horizontal by 45° angle.
A preferred embodiment of the invention, the putting hole are located at a light direction of the dichroscope
On；It is equipped with light source in the top of the reflecting mirror, is successively arranged fluorescence band logical on another light direction of the dichroscope
Optical filter, collector lens and photoelectric sensor, wherein the photoelectric sensor is connected with signal processing apparatus.
Based on the above-mentioned technical proposal, the fluorescence detection device of the embodiment of the present invention at least has the following technical effect that:
1, a kind of fluorescence detection device provided by the invention, compact, structure is simple, can carry at any time and to sample
Product are detected.
2, by rotating supporting body relative to pedestal, while the opening being connected with accommodating chamber is set on pedestal, side
Just it the taking-up of supporting body or is put into, convenient for placing sample to be tested in supporting body.
3, it by the multiple putting holes of setting being centrosymmetric in the top of supporting body about its center, can detect simultaneously
Multiple samples to be tested.
4, optical texture is simple, improves the transmission efficiency of exciting light and fluorescence, reduces optical path loss, improves light
Utilization rate improves detection effect.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the overall structure diagram of fluorescence detection device of the invention；
Fig. 2 is the schematic diagram of internal structure of fluorescence detection device of the invention；
Fig. 3 is the structural schematic diagram of the pedestal of fluorescence detection device of the invention；
Fig. 4 is the top view of the accommodating chamber of the pedestal of fluorescence detection device of the invention；
Fig. 5 is the sliding rail of fluorescence detection stake of the invention and the connection structure diagram of sliding block.
In figure: 10- sample placing modules；11- supporting body；12- top cover；13- optical de-tection means；14- signal processing device
It sets；15- outer housing；16- display device；17- switch gate；101- pedestal；102- slideway；103- peep hole；104- accommodating chamber；
105- opening；111- putting hole；112- sliding block；131- reflecting mirror；132- dichroscope；133- light source；The filter of 134- fluorescence band logical
Mating plate；135- collector lens；136- photoelectric sensor；137- fixing piece.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
1 to 5 pair of technical solution of the present invention is described in detail with reference to the accompanying drawings of the specification.
The present invention provides a kind of fluorescence detection devices, including outer housing 15, and sample placement group is equipped in outer housing 15
Part 10 and optical de-tection means 13.Sample placing modules 10 are for placing sample to be tested.Wherein, the bottom of sample placing modules 10
It forms a fixed connection with outer housing 15；Optical de-tection means 13 are connect to receive sample placing modules 10 with sample placing modules 10
Fluorescent and be translated into electric signal.To be detected to sample to be tested.
With reference to Fig. 2, Fig. 2 shows the schematic diagram of internal structures of fluorescence detection device of the invention.Further, sample is put
Component 10 is set to include pedestal 101 and be arranged on pedestal 101 and can be relative to the supporting body 11 of base rotation.In pedestal 101
Equipped with the accommodating chamber 104 for accommodating supporting body 11.The upper end of accommodating chamber 104 is open-type structure.So that supporting body 11 is put
It sets in accommodating chamber.Preferably, supporting body 11 is cylindrical structure.Preferably, accommodating chamber 104 is the shape phase with supporting body 11
Consistent and upper end opening cylindrical structure.
Further, the opening 105 being connected with accommodating chamber 104 is equipped on pedestal 101.Opening 105 is for making to carry
Body 11 is put into or is taken out in accommodating chamber 104.Preferably, opening 105 is arranged on the side wall of pedestal 101, convenient from the side of pedestal
Supporting body 11 is taken in and out accommodating chamber 104 by wall.Preferably, on the side wall of outer housing 15 and with 10 phase of sample placing modules
Corresponding position is equipped with switch gate 17, by turning on the switch door 17, to realize being put into or taking out for supporting body 11.
The top view of the accommodating chamber of the pedestal of fluorescence detection device of the invention is shown with reference to Fig. 4, Fig. 4.In order to realize
Rotation of the supporting body 11 relative to pedestal 101 is equipped with annular slide track 102 on the bottom surface of accommodating chamber 104.At the bottom of supporting body 11
Portion is equipped with sliding block 112, and sliding block 112 can be connected on slideway 102 and can slide along slideway 102, so that supporting body 11 passes through
Sliding block 112 is rotated relative to slideway 102, realizes the rotation of supporting body 11.
The structural schematic diagram of the pedestal of fluorescence detection device of the invention is shown with reference to Fig. 3, Fig. 3.In supporting body 11
Top is symmetrically equipped with multiple putting holes 111.Putting hole 111 is for placing sample to be tested.Preferably, multiple putting holes 111 close
It is centrosymmetric setting in the center of supporting body 11.Multiple samples are detected simultaneously to convenient.Putting hole 111 is set as
The mode being centrosymmetric, the first purpose are that whether observation sample to be tested aligns with optical de-tection means for convenience, are
It is no in the optical path of optical de-tection means.
Further, top cover 12 is additionally provided in the top of pedestal 101.Top cover 12 with it is rectangular in the accommodating chamber 104 of pedestal 101
At being fixedly connected.At least two peep holes 103 being centrosymmetric about the center of top cover 12 are equipped on top cover 12.It is preferred that
, the size of peep hole 103 is consistent with the size of putting hole 111.To be placed in accommodating chamber 104 by supporting body 11
Afterwards, rotate it along slideway 102 by stirring supporting body 11, until peep hole 103 and putting hole 111 are overlapped.Preferably, as schemed
Shown in 2, wherein optical de-tection means 13 are arranged in the surface of a peep hole 103 of inside, another peep hole
103 are centrosymmetric with it, therefore, when observing putting hole 111 in the peep hole 103 for being located at outside, then illustrate to place
The putting hole of sample to be tested is located in the optical path of optical de-tection means 13.Preferably, the top cover of outer housing 15 is transparence knot
Structure can be glass workpiece, facilitate whether observation sample to be tested is located in the optical path of photoelectric detection mechanism.
Further, with reference to Fig. 2, the top of top cover 12 is arranged in optical de-tection means 13.Also, optical de-tection means 13
Fixing piece 137 be arranged at least one peep hole 103 above, fixing piece 137 be cylinder hollow structure.To prevent from blocking
The transmitting of optical path.It can be interpreted as, optical de-tection means 13 are connected by fixing piece 137 with the accommodating chamber 104 of pedestal.
Further, dichroscope 132 is provided tiltedly in optical de-tection means 13 and right above fixing piece 137.
It is parallel with dichroscope 132 to be equipped with reflecting mirror 131, wherein dichroscope 132 and the inclination of reflecting mirror 131 horizontal by
45° angle.Preferably, putting hole 111 is located on a light direction of dichroscope 132.Preferably, putting hole 111 is located at two
To on the vertical optical path of Look mirror 132, it is located on the reflected light path of dichroscope 132 so as to the sample to be tested in putting hole 111.?
The top of reflecting mirror 131 is equipped with light source 133, and the mirror surface of chief ray and reflecting mirror 131 that light source issues is incident in 45° angle.It is preferred that
, dichroscope 132 is arranged on the reflected light path of reflecting mirror 131.It is successively set on another light direction of dichroscope 132
There are fluorescence bandpass filter 134, collector lens 135 and photoelectric sensor 136, wherein photoelectric sensor 136 and signal processing
Device 14 is connected.Preferably, display device 16 is equipped on outer housing, signal processing apparatus 14 is connected with display device 16
It connects, to be shown by display device 16 to fluorescence data detected.
Fluorescence detection device of the invention when in use, first takes out supporting body 11 from the opening 105 of pedestal 101, then
It is put into sample to be tested in the putting hole 111 of supporting body 11, then supporting body 11 is returned in accommodating chamber 104, and carries
The sliding block 112 of body 11 is stuck on slideway 102, manual toggle supporting body 11, to observe from the peep hole 103 for being located at 12 outside of top cover
When to putting hole 111, then optical de-tection means are opened, carries out fluorescence detection.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of fluorescence detection device, which is characterized in that including outer housing (15), put in the outer housing (15) equipped with sample
Set component (10) and optical de-tection means (13), wherein the sample placing modules (10) and the outer housing (15) are formed admittedly
Fixed connection；The optical de-tection means (13) connect with the sample placing modules (10) to receive the sample placing modules
(10) fluorescent is simultaneously translated into electric signal.
2. fluorescence detection device according to claim 1, which is characterized in that the sample placing modules (10) include pedestal
(101) and be arranged on the pedestal (101) and can relative to the pedestal (101) rotate supporting body (11), at the bottom
The accommodating chamber (104) for accommodating the supporting body (11) is equipped in seat (101), the upper end of the accommodating chamber (104) is opening
Type structure.
3. fluorescence detection device according to claim 2, which is characterized in that on the pedestal (101) be equipped with it is described
The opening (105) that accommodating chamber (104) is connected, the opening (105) is for making described in the supporting body (11) is put into or takes out
In accommodating chamber (104).
4. fluorescence detection device according to claim 3, which is characterized in that set on the bottom surface of the accommodating chamber (104)
There are annular slide track (102), is equipped with sliding block (112) in the bottom of the supporting body (11), the sliding block (112) can be connected to institute
It states on slideway (102), so that the supporting body (11) is rotated by the sliding block (112) relative to the slideway (102).
5. fluorescence detection device according to claim 4, which is characterized in that be equipped on the top of the supporting body (11) more
A putting hole (111), wherein multiple putting holes (111) are centrosymmetric setting about the center of the supporting body (11).
6. fluorescence detection device according to claim 5, which is characterized in that be additionally provided with above the pedestal (101)
It forms a fixed connection above top cover (12), the top cover (12) and the accommodating chamber (104) of the pedestal (101).
7. fluorescence detection device according to claim 6, which is characterized in that be equipped on the top cover (12) about described
At least two peep holes (103) that the center of top cover (12) is centrosymmetric, the size and the placement of the peep hole (103)
The size in hole (111) is consistent.
8. fluorescence detection device according to claim 7, which is characterized in that the optical de-tection means (13) are arranged in institute
The top of top cover (12) is stated, also, the fixing piece (137) of the optical de-tection means (13) is arranged at least one observation
Above hole (103), the fixing piece (137) is cylinder hollow structure.
9. fluorescence detection device according to claim 8, which is characterized in that and position interior in the optical de-tection means (13)
Dichroscope (132) are provided tiltedly with right above the fixing piece (137), parallel with the dichroscope (132) is equipped with
Reflecting mirror (131), wherein the dichroscope (132) and the reflecting mirror (131) are tilted horizontal by 45° angle.
10. fluorescence detection device according to claim 9, which is characterized in that be equipped in the top of the reflecting mirror (131)
Light source (133)；The putting hole (111) is located on a light direction of the dichroscope (132)；In the dichroscope
(132) fluorescence bandpass filter (134), collector lens (135) and photoelectric sensor are successively arranged on another light direction
(136), wherein the photoelectric sensor (136) is connected with signal processing apparatus (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811611863.7A CN109781680A (en) | 2018-12-27 | 2018-12-27 | A kind of fluorescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811611863.7A CN109781680A (en) | 2018-12-27 | 2018-12-27 | A kind of fluorescence detection device |
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| Publication Number | Publication Date |
|---|---|
| CN109781680A true CN109781680A (en) | 2019-05-21 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811611863.7A Pending CN109781680A (en) | 2018-12-27 | 2018-12-27 | A kind of fluorescence detection device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1815196A (en) * | 2005-02-03 | 2006-08-09 | 三星电子株式会社 | Multi-channel fluorescence measuring optical system and multi-channel fluorescence sample analyzer |
| CN102445439A (en) * | 2010-10-09 | 2012-05-09 | 北京网新易尚科技有限公司 | Chemiluminescence detection device |
| CN104076023A (en) * | 2014-07-22 | 2014-10-01 | 中国人民解放军第三军医大学第一附属医院 | Body fluid Raman spectrum testing device |
| CN205262944U (en) * | 2015-12-30 | 2016-05-25 | 天津速伦科技有限公司 | Detection platform of spectrum analyzer |
| CN208091919U (en) * | 2018-04-24 | 2018-11-13 | 桂林优利特医疗电子有限公司 | A kind of fluorescence detection device |
-
2018
- 2018-12-27 CN CN201811611863.7A patent/CN109781680A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1815196A (en) * | 2005-02-03 | 2006-08-09 | 三星电子株式会社 | Multi-channel fluorescence measuring optical system and multi-channel fluorescence sample analyzer |
| CN102445439A (en) * | 2010-10-09 | 2012-05-09 | 北京网新易尚科技有限公司 | Chemiluminescence detection device |
| CN104076023A (en) * | 2014-07-22 | 2014-10-01 | 中国人民解放军第三军医大学第一附属医院 | Body fluid Raman spectrum testing device |
| CN205262944U (en) * | 2015-12-30 | 2016-05-25 | 天津速伦科技有限公司 | Detection platform of spectrum analyzer |
| CN208091919U (en) * | 2018-04-24 | 2018-11-13 | 桂林优利特医疗电子有限公司 | A kind of fluorescence detection device |
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| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Tiezhu Inventor after: Zhang Jie Inventor after: Lv Chengwei Inventor after: Li Zhuolin Inventor before: Zhang Liang Inventor before: Zhao Tiegang |
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| CB03 | Change of inventor or designer information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190521 |
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| RJ01 | Rejection of invention patent application after publication |