CN113186082A - Microorganism detection system - Google Patents
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- CN113186082A CN113186082A CN202110356835.0A CN202110356835A CN113186082A CN 113186082 A CN113186082 A CN 113186082A CN 202110356835 A CN202110356835 A CN 202110356835A CN 113186082 A CN113186082 A CN 113186082A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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Abstract
The invention belongs to the field of microorganism detection, and particularly relates to a microorganism detection system which comprises an analysis module, a culture dish, a constant temperature device and a detection device; the analysis module is connected with the constant temperature device and the detection device through a network and is used for data transmission; the analysis module processes and analyzes the data; the culture dish is used for culturing the growth of microorganisms; the constant temperature device is used for controlling the temperature of the microorganism growth environment; the detection device is used for comparing the concentration of the microorganisms in the culture dish; the culture dish is driven by the turntable to be positioned under the light emitter, and then the visible light emitted by the light emitter passes through the culture dish and is received and matched by the photosensitive sheet, so that the analysis module performs comparison and analysis on the detection result, the concentration difference among a plurality of microbial samples to be detected can be rapidly compared, and the invention has the advantages of convenience, rapidness and high working efficiency.
Description
Technical Field
The invention belongs to the field of microbial detection, and particularly relates to a microbial detection system.
Background
In order to provide scientific basis for various health management works and provide prevention and treatment measures for infectious diseases and human, animal and food poisoning, the detection of microorganisms is often required, which is different from the accurate requirements of a biological laboratory or related departments on the detection of the concentration of the microorganisms, and most of the detection of the microorganisms only needs to be capable of comparing the concentration of a microorganism detection sample with the concentration of a microorganism standard sample; due to the defects of the prior art, the traditional microorganism detection methods such as a volume measurement method and a dry weight method need to firstly centrifuge a culture solution to be detected of microorganisms for a period of time, pour out the centrifuged supernatant for volume measurement or dry weighing, have high detection working strength, take long time and need relatively precise equipment, and can not meet the requirements of people on simple and rapid comparison of concentrations of different microorganism concentrations.
Some technical schemes related to microorganism detection also appear in the prior art, for example, a chinese patent with application number CN201921209629.1 discloses a microorganism concentration detection system, which comprises a processor, a high efficiency filter, a microorganism mass concentration acquisition mechanism, a microorganism number concentration acquisition mechanism, a flow meter and a pump, wherein the high efficiency filter is connected with an air inlet of the microorganism mass concentration acquisition mechanism through a three-way electromagnetic valve, a part of the gas flowing out from an air outlet of the microorganism mass concentration acquisition mechanism directly flows out through a pipeline, the pipeline is provided with a flow meter i and a pump i, the other part of the gas directly flows into the air inlet of the microorganism number concentration acquisition mechanism, the pipeline connected with the air outlet of the microorganism number concentration acquisition mechanism is provided with a flow meter ii and a pump ii, and the microorganism mass concentration acquisition mechanism, the microorganism number concentration acquisition mechanism, the flow meter and the pump are all electrically connected with the processor, the structure is simple, the sensitivity is high, and the accuracy of the finally obtained detection result is high; but the problem of the concentration height of two little biological detection appearance of rapid contrast still can not be solved to this technique, can not improve the efficiency that little biological concentration contrast detected to can not satisfy the demand of rapid contrast between the microorganism concentration, and then cause the limitation of this scheme.
In view of the above, in order to overcome the above technical problems, the present invention provides a microorganism detection system, which solves the above technical problems.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a microorganism detection system, a culture dish is driven by a turntable to be positioned under a light emitter, and then the culture dish and visible light emitted by the light emitter pass through the culture dish and are received and matched by a photosensitive sheet, so that an analysis module carries out contrastive analysis on a detection result, the concentration difference among a plurality of microorganism samples to be detected can be rapidly contrasted, and the microorganism detection system has the advantages of convenience, rapidness and high working efficiency.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a microorganism detection system, which comprises an analysis module, a culture dish, a constant temperature device and a detection device; the analysis module is connected with the constant temperature device and the detection device through a network and is used for data transmission; the analysis module processes and analyzes the data; the culture dish is formed by clamping an upper disc and a lower disc and is used for culturing the growth of microorganisms; the constant temperature device is used for controlling the temperature of the microorganism growth environment; the detection device is used for comparing the concentration of the microorganisms in the culture dish;
the detection device comprises a base, a turntable, a light emitter, a photosensitive film and a controller; the upper end of the base is fixedly connected with a first electric push rod; one end of the first electric push rod is fixedly connected with a connecting plate; the other end of the connecting plate is fixedly connected with a light emitter; the light emitter is used for emitting visible light, and the emitting end of the light emitter faces the base; the outer surface of the transmitting end of the light emitter is fixedly connected with a first light shield; a first motor is installed at the upper end of the base; the output shaft of the first motor is fixedly connected with a turntable; through holes are uniformly formed in the upper end of the rotary table; the through hole is positioned right below the light emitter after being rotated by the turntable; the culture dish is placed in the through hole; the first light shield is used for shielding light from the outside between the light emitter and the through hole; the upper end of the base is fixedly connected with a photosensitive sheet; the photosensitive film is positioned right below the light emitter; a second light shield is sleeved outside the photosensitive film; the second light shield is used for shielding light rays from the outside between the through hole and the photosensitive film; the controller is used for controlling the detection device to automatically operate;
during working, due to the defects of the prior art, the traditional microorganism detection methods such as a volume measurement method and a dry weight method need to firstly centrifuge a culture solution to be detected of microorganisms for a period of time, pour out the centrifuged supernatant for volume measurement or dry weighing, have high detection working strength, take long time and need more precise equipment, and can not meet the requirement of people on simple and rapid comparison of concentrations of different microorganism concentrations;
therefore, the culture dish is placed in the through hole of the rotary table by a worker, the controller is started, the controller controls the first electric push rod to drive the connecting plate, the light emitter and the first light shield to slide towards the direction far away from the rotary table, the controller controls the first motor to rotate, the output shaft of the first motor drives the rotary table to synchronously rotate when rotating, the culture dish is driven to be positioned under the light emitter when the rotary table rotates, the controller controls the first electric push rod to drive the connecting plate, the light emitter and the first light shield to slide towards the rotary table until the bottom end of the first light shield is attached to the upper surface of the rotary table, the controller controls the light emitter to emit visible light, the visible light emitted by the light emitter passes through the culture dish and is received by the light sensing sheet, when the visible light emitted by the light emitter passes through the culture dish, partial light is blocked by microorganisms and cannot pass through, so that the light intensity received on the light sensing sheet is influenced by the concentration of the microorganisms in the culture dish, the lower the light intensity received by the photosensitive chip is, the higher the microorganism concentration is, the higher the light intensity received by the photosensitive chip is, the lower the microorganism concentration is, the optical signal received by the photosensitive chip is converted into an electric signal and then transmitted to the analysis module, and the analysis module compares and analyzes the data with the standard sample stored in advance to obtain the concentration of the microorganism detection sample, which is higher than the concentration of the standard sample;
according to the invention, the detection result of the microorganism detection sample and the data of the standard sample determined in advance are compared and analyzed, the culture dish can be driven by the turntable to be positioned under the light emitter, and then the visible light emitted by the light emitter passes through the culture dish and is received and matched by the photosensitive film, so that the analysis module can compare and analyze the detection result, and the concentration difference among a plurality of microorganism samples to be detected can be rapidly compared, and further the invention has the advantages of convenience, rapidness and high working efficiency.
Preferably, first grooves are symmetrically formed in the inner side wall of the through hole; a clamping unit is arranged in the first groove; the clamping unit comprises a second electric push rod and an arc-shaped clamp; the bottom of the first groove is fixedly connected with a second electric push rod; one end of the second electric push rod is provided with an arc-shaped clamp; the arc-shaped clamp is connected in the first groove in a sliding manner; the two arc-shaped clamps move close to each other after being pushed by the corresponding second electric push rods; during operation, the culture dish is placed in the through hole in the rotary table, the controller controls the second electric push rod to drive the two arc clamps to clamp the culture dish, so that visible light beams emitted by the light emitter vertically penetrate through the culture dish, light refraction caused by inclined placement of the culture dish is avoided, light signal change caused by light refraction is avoided, experimental errors are reduced, accuracy of the detection device is improved, meanwhile, the culture dish is clamped and fixed by the arc clamps, culture dishes of different specifications are conveniently clamped, practicability of the detection device is improved, and using effect of a microorganism detection system is enhanced.
Preferably, a second motor is arranged in the first groove; the second motor is positioned between the second electric push rod and the arc-shaped clamp; one side of the arc-shaped clamp recess is an inner side wall; the second motor is fixedly connected to one end of the second electric push rod, and an output shaft of the second motor is fixedly connected with the outer side wall of the arc-shaped clamp; when the microorganism detection device works, after the arc clamp clamps the culture dish, the controller controls the output shaft of the second motor to drive the culture dish to rotate, so that microorganisms in the culture dish are uniformly shaken and distributed discretely when the culture dish rotates, experimental errors possibly caused by accumulation and bundling of the microorganisms sampled in the culture dish are avoided, the accuracy of the detection device is improved, and the use effect of the microorganism detection system is enhanced.
Preferably, the inner side wall of the through hole is symmetrically provided with a second groove; a cleaning unit is arranged in the second groove; the cleaning unit comprises a spring, a connecting plate and a cleaning sponge; the connecting plate is connected in the second groove in a sliding manner; one end of the spring is fixedly connected to the bottom of the second groove, and the other end of the spring is fixedly connected with the connecting plate; a cleaning sponge is arranged on the connecting plate; when the device works, the controller controls the second motor to rotate, the second motor output shaft drives the culture dish to rotate, the cleaning sponge is attached to the outer surface of the culture dish under the action of the spring, and when the culture dish rotates, the cleaning sponge uniformly cleans the outer surface of the culture dish under the action of the spring, so that the phenomenon that visible light penetrates due to the fact that impurities exist on the outer surface of the culture dish to be influenced is avoided, experimental errors are reduced, and the accuracy of the detection device is improved.
Preferably, the cross section of the connecting plate is U-shaped, and a roller is rotatably connected between the two inner side walls of the connecting plate; the cleaning sponge is sleeved on the outer wall of the roller; the cleaning sponge is attached to the outer wall of the culture dish under the action of the spring; when the device works, the cleaning sponge is attached to the outer surface of the culture dish in a rolling mode through the rolling rod, sliding friction between the cleaning sponge and the culture dish is changed into rolling friction, so that the wear rate of the culture dish is reduced, the acting area of the cleaning sponge is increased, the cleaning effect of the cleaning unit is enhanced, the accuracy of the detection device is improved, and the service life of the culture dish is prolonged.
Preferably, a strip-shaped groove is formed in the inner side wall of the arc-shaped clamp; the section of the strip-shaped groove is V-shaped; two adjacent strip-shaped grooves are arranged in a crossed manner; when the device works, after the culture dish is clamped by the arc clamp, the contact area between the inner side wall of the arc clamp and the outer side wall of the culture dish is reduced through the strip-shaped groove arranged on the inner side wall of the arc clamp, and the pressure intensity of the outer side wall of the culture dish is increased because the extrusion force exerted on the outer side wall of the culture dish is unchanged and the contact area is reduced, so that the friction force between the arc clamp and the culture dish is increased, the stability of the detection device is improved, and the use effect of the microorganism detection system is enhanced.
The invention has the following beneficial effects:
1. the culture dish is driven by the turntable to be positioned under the light emitter, and then the visible light emitted by the light emitter passes through the culture dish and is received and matched by the photosensitive sheet, so that the analysis module performs comparison and analysis on the detection result, the concentration difference among a plurality of microbial samples to be detected can be rapidly compared, and the invention has the advantages of convenience, rapidness and high working efficiency.
2. When the detection device provided by the invention is used for detection, the culture dish is placed in the through hole in the turntable, the controller controls the second electric push rod to drive the two arc-shaped clamps to clamp the culture dish, so that visible light beams emitted by the light emitter vertically pass through the culture dish, light refraction caused by inclined placement of the culture dish is avoided, light signal change caused by light refraction is avoided, experimental errors are reduced, the accuracy of the detection device provided by the invention is improved, meanwhile, the culture dish is clamped and fixed by the arc-shaped clamps 232, culture dishes of different specifications are also conveniently clamped, the practicability of the detection device provided by the invention is improved, and the using effect of a microorganism detection system is enhanced.
3. When the detection device provided by the invention is used for detecting, after the culture dish is clamped by the arc-shaped clamp, the output shaft of the second motor is controlled by the controller to drive the culture dish to rotate, so that microorganisms in the culture dish are uniformly shaken and discretely distributed when the culture dish rotates, the possible experimental error caused by the accumulation and the piling of the microorganisms sampled in the culture dish is avoided, the accuracy of the detection device provided by the invention is improved, and the use effect of a microorganism detection system is enhanced.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a perspective view of the detection device of the present invention;
FIG. 2 is a top view of the detection device of the present invention;
FIG. 3 is a cross-sectional view A-A of FIG. 2;
FIG. 4 is a cross-sectional view B-B of FIG. 2;
FIG. 5 is an enlarged view at C of FIG. 3;
FIG. 6 is an enlarged view at D of FIG. 4;
in the figure: the device comprises a base 1, a turntable 2, a through hole 21, a first groove 22, a clamping unit 23, a second electric push rod 231, an arc clamp 232, a second motor 233, a strip-shaped groove 234, a second groove 24, a cleaning unit 25, a spring 251, a connecting plate 252, a cleaning sponge 253, a rolling rod 254, a first electric push rod 3, a connecting plate 4, a light emitter 5, a first light shield 6, a first motor 7, a light sensing sheet 8 and a second light shield 9.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 6, a microorganism detection system according to the present invention includes an analysis module, a culture dish, a thermostat, and a detection device; the analysis module is connected with the constant temperature device and the detection device through a network and is used for data transmission; the analysis module processes and analyzes the data; the culture dish is formed by clamping an upper disc and a lower disc and is used for culturing the growth of microorganisms; the constant temperature device is used for controlling the temperature of the microorganism growth environment; the detection device is used for comparing the concentration of the microorganisms in the culture dish;
the detection device comprises a base 1, a turntable 2, a light emitter 5, a photosensitive film 8 and a controller; the upper end of the base 1 is fixedly connected with a first electric push rod 3; one end of the first electric push rod 3 is fixedly connected with a connecting plate 4; the other end of the connecting plate 4 is fixedly connected with a light emitter 5; the light emitter 5 is used for emitting visible light, and the emitting end of the light emitter 5 faces the base 1; the outer surface of the transmitting end of the light emitter 5 is fixedly connected with a first light shield 6; the upper end of the base 1 is provided with a first motor 7; an output shaft of the first motor 7 is fixedly connected with a rotary disc 2; through holes 21 are uniformly formed in the upper end of the rotary table 2; the through hole 21 is positioned under the light emitter 5 after being rotated by the turntable 2; the culture dish is placed in the through hole 21; the first light shield 6 is used for shielding light from the outside between the light emitter 5 and the through hole 21; the upper end of the base 1 is fixedly connected with a photosensitive sheet 8; the photosensitive sheet 8 is positioned right below the light emitter 5; a second light shield 9 is sleeved outside the photosensitive film 8; the second light shield 9 is used for shielding light rays from the outside between the through hole 21 and the light sensing sheet 8; the controller is used for controlling the detection device to automatically operate;
during working, due to the defects of the prior art, the traditional microorganism detection methods such as a volume measurement method and a dry weight method need to firstly centrifuge a culture solution to be detected of microorganisms for a period of time, pour out the centrifuged supernatant for volume measurement or dry weighing, have high detection working strength, take long time and need more precise equipment, and can not meet the requirement of people on simple and rapid comparison of concentrations of different microorganism concentrations;
therefore, the culture dish is placed in the through hole 21 of the rotary table 2 by a worker, the controller is started, the controller controls the first electric push rod 3 to drive the connecting plate 4, the light emitter 5 and the first light shield 6 to slide towards the direction far away from the rotary table 2, the controller controls the first motor 7 to rotate, the output shaft of the first motor 7 drives the rotary table 2 to synchronously rotate when rotating, the culture dish is driven to be positioned under the light emitter 5 when the rotary table 2 rotates, at the moment, the controller controls the first electric push rod 3 to drive the connecting plate 4, the light emitter 5 and the first light shield 6 to slide towards the rotary table 2 until the bottom end of the first light shield 6 is attached to the upper surface of the rotary table 2, the controller controls the light emitter 5 to emit visible light, the visible light emitted by the light emitter 5 passes through the culture dish and is received by the light sensing sheet 8, and when the visible light emitted by the light emitter 5 passes through the culture dish, part of the light is blocked by microorganisms and cannot pass through, the light intensity received by the light-sensitive sheet 8 is influenced by the concentration of microorganisms in the culture dish, the lower the light intensity received by the light-sensitive sheet 8 is, the higher the concentration of the microorganisms is, the higher the light intensity received by the light-sensitive sheet 8 is, the lower the concentration of the microorganisms is, the light signals received by the light-sensitive sheet 8 are converted into electric signals and then transmitted to the analysis module, and the analysis module compares and analyzes the electric signals with the data of the standard sample stored in advance to obtain the concentration of the microorganism detection sample, which is higher than the concentration of the standard sample;
according to the invention, the culture dish is driven by the turntable 2 to be positioned under the light emitter 5, and then the visible light emitted by the light emitter 5 passes through the culture dish and is received and matched by the photosensitive sheet 8, so that the analysis module carries out comparison and analysis on the detection result, and the concentration difference among a plurality of microbial samples to be detected can be rapidly compared, and further, the invention has the advantages of convenience, rapidness and high working efficiency.
As an embodiment of the present invention, a first groove 22 is symmetrically arranged on the inner side wall of the through hole 21; a clamping unit 23 is arranged in the first groove 22; the clamping unit 23 comprises a second electric push rod 231 and an arc-shaped clamp 232; the groove bottom of the first groove 22 is fixedly connected with a second electric push rod 231; one end of the second electric push rod 231 is provided with an arc-shaped clamp 232; the arc-shaped clamp 232 is connected in the first groove 22 in a sliding mode; the two arc-shaped clamps 232 move close to each other after being pushed by the corresponding second electric push rods 231; during operation, the culture dish is placed in the through hole 21 in the turntable 2, the controller controls the second electric push rod 231 to drive the two arc-shaped clamps 232 to clamp the culture dish, so that visible light beams emitted by the light emitter 5 vertically penetrate through the culture dish, light refraction caused by inclined placement of the culture dish is avoided, light signal change caused by light refraction is avoided, experimental errors are reduced, accuracy of the detection device is improved, meanwhile, the culture dish is clamped and fixed by the arc-shaped clamps 232, culture dishes of different specifications are convenient to clamp, practicability of the detection device is improved, and using effect of a microorganism detection system is enhanced.
As an embodiment of the present invention, a second motor 233 is disposed in the first groove 22; the second motor 233 is positioned between the second electric push rod 231 and the arc-shaped clamp 232; one side of the arc-shaped clamp 232 which is sunken is an inner side wall; the second motor 233 is fixedly connected to one end of the second electric push rod 231, and an output shaft of the second motor 233 is fixedly connected with the outer side wall of the arc-shaped clamp 232; when the microorganism detection device works, after the arc clamp 232 clamps the culture dish, the controller controls the output shaft of the second motor 233 to drive the culture dish to rotate, so that microorganisms in the culture dish are uniformly shaken and distributed discretely when the culture dish rotates, experimental errors possibly caused by accumulation and bundling of the microorganisms sampled in the culture dish are avoided, the accuracy of the detection device is improved, and the use effect of the microorganism detection system is enhanced.
As an embodiment of the invention, a second groove 24 is symmetrically arranged on the inner side wall of the through hole 21; a cleaning unit 25 is arranged in the second groove 24; the cleaning unit 25 includes a spring 251, a connection plate 252, and a cleaning sponge 253; the connecting plate 252 is connected in the second groove 24 in a sliding manner; one end of the spring 251 is fixedly connected with the bottom of the second groove 24, and the other end of the spring 251 is fixedly connected with the connecting plate 252; a cleaning sponge 253 is arranged on the connecting plate 252; when the device works, the controller controls the second motor 233 to rotate, the output shaft of the second motor 233 drives the culture dish to rotate, the cleaning sponge 253 is attached to the outer surface of the culture dish under the action of the spring 251, and when the culture dish rotates, the cleaning sponge 253 uniformly wipes the outer surface of the culture dish under the action of the spring 251, so that the phenomenon that visible light penetrates due to the fact that impurities exist on the outer surface of the culture dish is avoided, experimental errors are reduced, and the accuracy of the detection device is improved.
As an embodiment of the present invention, the connection plate 252 has a U-shaped cross section, and a roller 254 is rotatably connected between two inner sidewalls of the connection plate 252; the cleaning sponge 253 is sleeved on the outer wall of the roller 254; in one embodiment of the present invention, a strip-shaped groove 234 is disposed on the inner sidewall of the arc-shaped clamp 232; the section of the strip-shaped groove 234 is V-shaped; two adjacent strip-shaped grooves 234 are arranged in a crossing manner; when the device works, after the culture dish is clamped by the arc clamp 232, the contact area between the inner side wall of the arc clamp 232 and the outer side wall of the culture dish is reduced through the strip-shaped groove 234 arranged on the inner side wall of the arc clamp 232, and because the extrusion force applied to the outer side wall of the culture dish is unchanged and the contact area is reduced, the pressure applied to the outer side wall of the culture dish is increased, so that the friction force between the arc clamp 232 and the culture dish is increased, the clamping effect of the arc clamp on the culture dish is enhanced, the stability of the detection device is improved, and the use effect of the microorganism detection system is enhanced.
When the culture dish is used, a worker places the culture dish in the through hole 21 of the rotary table 2, the controller is started, the controller controls the first electric push rod 3 to drive the connecting plate 4, the light emitter 5 and the first light shield 6 to slide towards the direction far away from the rotary table 2, the controller controls the first motor 7 to rotate, the output shaft of the first motor 7 drives the rotary table 2 to synchronously rotate when rotating, the culture dish is driven to be positioned under the light emitter 5 when the rotary table 2 rotates, at the moment, the controller controls the first electric push rod 3 to drive the connecting plate 4, the light emitter 5 and the first light shield 6 to slide towards the rotary table 2 until the bottom end of the first light shield 6 is attached to the upper surface of the rotary table 2, the controller controls the light emitter 5 to emit visible light, the visible light emitted by the light emitter 5 passes through the culture dish and is received by the light sensing sheet 8, and when the visible light emitted by the light emitter 5 passes through the culture dish, part of the light is blocked by microorganisms and cannot pass through, the light intensity received by the light-sensitive sheet 8 is influenced by the concentration of microorganisms in the culture dish, the lower the light intensity received by the light-sensitive sheet 8 is, the higher the concentration of the microorganisms is, the higher the light intensity received by the light-sensitive sheet 8 is, the lower the concentration of the microorganisms is, the light signals received by the light-sensitive sheet 8 are converted into electric signals and then transmitted to the analysis module, and the analysis module compares and analyzes the electric signals with the data of the standard sample stored in advance to obtain the concentration of the microorganism detection sample, which is higher than the concentration of the standard sample; the culture dish is placed in the through hole 21 in the rotary table 2, the controller controls the second electric push rod 231 to drive the two arc-shaped clamps 232 to clamp the culture dish, so that visible light beams emitted by the light emitter 5 vertically penetrate through the culture dish, light refraction caused by inclined placement of the culture dish is avoided, light signal change caused by light refraction is avoided, experimental errors are reduced, accuracy of the detection device is improved, meanwhile, the culture dish is clamped and fixed by the arc-shaped clamps 232, culture dishes of different specifications are convenient to clamp, practicability of the detection device is improved, and using effect of a microorganism detection system is enhanced; after the arc clamp 232 clamps the culture dish, the controller controls the output shaft of the second motor 233 to drive the culture dish to rotate, so that microorganisms in the culture dish are uniformly shaken and discretely distributed when the culture dish rotates, experimental errors possibly caused by accumulation and bundling of the microorganisms sampled in the culture dish are avoided, the accuracy of the detection device is improved, and the use effect of a microorganism detection system is enhanced; the controller controls the second motor 233 to rotate, the output shaft of the second motor 233 drives the culture dish to rotate, the cleaning sponge 253 is attached to the outer surface of the culture dish under the action of the spring 251, and when the culture dish rotates, the cleaning sponge 253 uniformly wipes the outer surface of the culture dish under the action of the spring 251, so that the influence of impurities on the outer surface of the culture dish on passing of visible light is avoided, the experimental error is reduced, and the accuracy of the detection device is improved; the cleaning sponge 253 is attached to the outer surface of the culture dish in a rolling manner through the rolling rod 254, so that sliding friction between the cleaning sponge 253 and the culture dish is changed into rolling friction, the wear rate of the culture dish is reduced, the action area of the cleaning sponge 253 is increased, the cleaning effect of the cleaning unit 25 is enhanced, the accuracy of the detection device is improved, and the service life of the culture dish is prolonged; after the culture dish is clamped by the arc clamp 232, the contact area between the inner side wall of the arc clamp 232 and the outer side wall of the culture dish is reduced through the strip-shaped groove 234 arranged on the inner side wall of the arc clamp 232, and the contact area is reduced because the extrusion force exerted on the outer side wall of the culture dish is unchanged, so that the pressure exerted on the outer side wall of the culture dish is increased, and the friction force between the arc clamp 232 and the culture dish is increased, so that the clamping effect of the arc clamp on the culture dish is enhanced, the stability of the detection device is improved, and the use effect of the microorganism detection system is enhanced.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A microorganism detection system comprises an analysis module, a culture dish and a constant temperature device, and is characterized in that: the microorganism detection system also comprises a detection device; the analysis module is connected with the constant temperature device and the detection device through a network and is used for data transmission; the analysis module processes and analyzes the data; the culture dish is formed by clamping an upper disc and a lower disc and is used for culturing the growth of microorganisms; the constant temperature device is used for controlling the temperature of the microorganism growth environment; the detection device is used for comparing the concentration of the microorganisms in the culture dish;
the detection device comprises a base (1), a turntable (2), a light emitter (5), a light sensing sheet (8) and a controller; the upper end of the base (1) is fixedly connected with a first electric push rod (3); one end of the first electric push rod (3) is fixedly connected with a connecting plate (4); the other end of the connecting plate (4) is fixedly connected with a light emitter (5); the light emitter (5) is used for emitting visible light, and the emitting end of the light emitter (5) faces the base (1); the outer surface of the transmitting end of the light emitter (5) is fixedly connected with a first light shield (6); a first motor (7) is installed at the upper end of the base (1); an output shaft of the first motor (7) is fixedly connected with a turntable (2); through holes (21) are uniformly formed in the upper end of the rotary table (2); the through hole (21) is positioned right below the light emitter (5) after being rotated by the turntable (2); the culture dish is placed in the through hole (21); the first light shield (6) is used for shielding light from the outside between the light emitter (5) and the through hole (21); the upper end of the base (1) is fixedly connected with a photosensitive sheet (8); the photosensitive sheet (8) is positioned right below the light emitter (5); a second light shield (9) is sleeved outside the photosensitive film (8); the second light shield (9) is used for shielding light rays from the outside between the through hole (21) and the photosensitive film (8); the controller is used for controlling the automatic operation of the detection device.
2. A microbiological detection system according to claim 1 wherein: a first groove (22) is symmetrically arranged on the inner side wall of the through hole (21); a clamping unit (23) is arranged in the first groove (22); the clamping unit (23) comprises a second electric push rod (231) and an arc-shaped clamp (232); the bottom of the first groove (22) is fixedly connected with a second electric push rod (231); one end of the second electric push rod (231) is provided with an arc-shaped clamp (232); the arc-shaped clamp (232) is connected in the first groove (22) in a sliding manner; the two arc-shaped clamps (232) move close to each other after being pushed by the corresponding second electric push rods (231).
3. A microbiological detection system according to claim 2 wherein: a second motor (233) is arranged in the first groove (22); the second motor (233) is positioned between the second electric push rod (231) and the arc-shaped clamp (232); one side of the arc-shaped clamp (232) which is sunken is an inner side wall; the second motor (233) is fixedly connected to one end of the second electric push rod (231), and an output shaft of the second motor (233) is fixedly connected with the outer side wall of the arc-shaped clamp (232).
4. A microbiological detection system according to claim 3 wherein: a second groove (24) is symmetrically arranged on the inner side wall of the through hole (21); a cleaning unit (25) is arranged in the second groove (24); the cleaning unit (25) comprises a spring (251), a connecting plate (252) and a cleaning sponge (253); the connecting plate (252) is connected in the second groove (24) in a sliding manner; one end of the spring (251) is fixedly connected with the bottom of the second groove (24), and the other end of the spring is fixedly connected with the connecting plate (252); the connecting plate (252) is provided with a cleaning sponge (253).
5. A microbiological detection system according to claim 4 wherein: the cross section of the connecting plate (252) is U-shaped, and a roller (254) is rotatably connected between the two inner side walls of the connecting plate (252); the cleaning sponge (253) is sleeved on the outer wall of the roller (254); the cleaning sponge (253) is attached to the outer wall of the culture dish under the action of a spring (251).
6. A microbiological detection system according to claim 5 wherein: a strip-shaped groove (234) is formed in the inner side wall of the arc-shaped clamp (232); the section of the strip-shaped groove (234) is V-shaped; two adjacent strip-shaped grooves (234) are arranged in a crossing manner.
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| CN202110356835.0A CN113186082A (en) | 2021-04-01 | 2021-04-01 | Microorganism detection system |
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| CN202110356835.0A CN113186082A (en) | 2021-04-01 | 2021-04-01 | Microorganism detection system |
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Cited By (1)
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