CN214361382U - Data analysis device for digital PCR fluorescence detector with high analysis efficiency - Google Patents

Data analysis device for digital PCR fluorescence detector with high analysis efficiency Download PDF

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Publication number
CN214361382U
CN214361382U CN202022306358.0U CN202022306358U CN214361382U CN 214361382 U CN214361382 U CN 214361382U CN 202022306358 U CN202022306358 U CN 202022306358U CN 214361382 U CN214361382 U CN 214361382U
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slide rail
digital pcr
data analysis
fluorescence detector
standard liquid
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CN202022306358.0U
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Chinese (zh)
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荣章权
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Zhengzhou University
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Zhengzhou University
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Abstract

The utility model discloses a data analysis device for digital PCR fluorescence detector that analysis efficiency is high, the on-line screen storage device comprises a base, the top of base is provided with the casing, and is provided with standard liquid on the terminal surface of casing and places the mouth, standard liquid is placed and is provided with standard liquid dish in the mouth, and the top of standard liquid dish is provided with the support, the upper end of support is provided with the air pump, and the below of air pump is provided with lead screw elevator motor, lead screw elevator motor's below is provided with the lifter plate, is provided with on the lifter plate and gets the liquid test tube, the lower extreme of support is provided with first slide rail, is provided with first gear motor on the first slide rail, and one side of first slide rail is provided with the second slide rail, is provided with on the second slide rail and receives the model. This data analysis device for digital PCR fluorescence detector that analysis efficiency is high gets the liquid test tube and controls through the electric cabinet that sets up and get the liquid test tube through setting up the multiunit, can reach and change the purpose that the sample group number detected improvement inspection efficiency.

Description

Data analysis device for digital PCR fluorescence detector with high analysis efficiency
Technical Field
The utility model relates to a data analysis technical field specifically is a data analysis device for digital PCR fluorescence detector that analysis efficiency is high.
Background
The fluorescent quantitative PCR detector is an instrument for labeling and tracking a PCR product and monitoring the reaction process in real time by a fluorescent dye or a fluorescent labeled specific probe, can perform qualitative and quantitative analysis on the product by combining corresponding software, calculate the initial concentration of a sample template to be detected, add a fluorescent group into a PCR reaction system, monitor the whole PCR process in real time by using fluorescent signal accumulation, and finally perform quantitative analysis on an unknown template by a standard curve, wherein the PCR can relatively quantify the abundance of a specific gene or species, compare the difference between different treatments or samples, such as gene copy number and expression quantity to research the response of gene expression to the treatment, and simultaneously can obtain information such as PCR amplification efficiency and the like, the fluorescent quantitative PCR detector is mainly widely applied to the fields of genetic engineering research, medical research and the like and is very important to the detection of pathogens and medicaments, therefore, a data analysis device for a digital PCR fluorescence detector having high analysis efficiency is very important.
The prior art has the following defects or problems:
the existing data analysis device for the digital PCR fluorescence detector adopts manual titration detection analysis, the manual operation is slow, errors can occur, the amount of each time of the absorption is different, and therefore the result error is caused, reaction units of the existing digital PCR detector are few, the dynamic range of the digital PCR is narrow, the working efficiency is low, and when the existing digital PCR detector detects the configured standard solution, all the reaction units are added into the standard solution, the number of the used reaction units can not be changed at will, so that when the reaction units are not needed according to the requirements, the reaction units are required to be detected and analyzed due to the fact that the number of the reaction units can not be changed, the working amount of the detection effect is increased, and the existing digital PCR detector has the problem of low working efficiency.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to prior art's weak point, provide a data analysis device for digital PCR fluorescence detector that analysis efficiency is high to solve the problem that the data analysis device work efficiency is low for the digital PCR fluorescence detector that proposes in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: a data analysis device for a digital PCR fluorescence detector with high analysis efficiency comprises a base, wherein a shell is arranged above the base, a standard liquid placing opening is formed in one end face of the shell, a standard liquid disc is arranged in the standard liquid placing opening, a support is arranged above the standard liquid disc, an air pump is arranged at the upper end of the support, a lead screw lifting motor is arranged below the air pump, a lifting plate is arranged below the lead screw lifting motor, a liquid taking test tube is arranged on the lifting plate, a first sliding rail is arranged at the lower end of the support, a first speed reducing motor is arranged on the first sliding rail, a second sliding rail is arranged on one side of the first sliding rail, a sample collecting plate is arranged on the second sliding rail, a sample collecting pit is arranged on the sample collecting plate, a second speed reducing motor is arranged on the second sliding rail, and an ultraviolet detector is arranged on one side of the sample collecting plate, and a detection inlet is arranged on one end face of the ultraviolet detector close to the sample receiving plate, and an electric cabinet is arranged at the upper end of the shell.
As the utility model discloses an optimized technical scheme, the internal diameter size phase-match of mouth is placed to the external diameter size and the standard liquid of standard liquid dish, and the standard liquid dish passes through the spout and places a mouthful sliding connection with the standard liquid.
As the utility model discloses a preferred technical scheme, the support passes through pulley and first slide rail sliding connection, and receives the model and pass through pulley and second slide rail sliding connection.
As the utility model discloses an optimal technical scheme, lead screw elevator motor passes through lead screw and lifter plate fixed connection, and the lifter plate passes through spout and support sliding connection.
As the utility model discloses an optimized technical scheme, the level of second slide rail is a little higher than the level of first slide rail, and gets the level of liquid test tube bottom and a little higher than the level of receiving the model.
As the utility model discloses a preferred technical scheme, the figure of getting the liquid test tube is fourteen groups, and fourteen groups get the liquid test tube and divide two rows of equidistance to fix on the lifter plate.
As an optimal technical scheme of the utility model, it is linked together through trachea and air pump to get the liquid test tube, and the position of getting the liquid test tube is corresponding with the position of receiving the appearance nest.
Compared with the prior art, the utility model provides a data analysis device for digital PCR fluorescence detector that analysis efficiency is high possesses following beneficial effect:
this data analysis device for digital PCR fluorescence detector that analysis efficiency is high, get the liquid test tube through setting up the multiunit, and get the liquid test tube with the multiunit and divide into two rows, the lead screw elevator motor that the rethread set up changes the height of getting the liquid test tube, join in marriage again and the air pump makes and gets the liquid test tube and inhale getting in the liquid test tube with the standard solution that the liquid dish was configured, the air pump of setting takes separate control to two rows of liquid test tubes of getting, can select one of them row of getting the liquid test tube to draw standard solution or all get the liquid test tube and all draw standard solution through the electric cabinet, thereby can change the group number of the standard solution of placing on the sampling plate, the purpose that has reached change reaction unit has reduced the work load that will detect, thereby the work efficiency who detects has been improved.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a schematic view of the sample collection structure of the present invention;
fig. 3 is the schematic diagram of the liquid-extracting structure of the present invention.
In the figure: 1. a base; 2. a housing; 3. a standard solution placing port; 4. a standard liquid pan; 5. a support; 6. an air pump; 7. a screw rod lifting motor; 8. a lifting plate; 9. taking a liquid test tube; 10. a first slide rail; 11. a first reduction motor; 12. a second slide rail; 13. collecting a sample plate; 14. collecting a sample nest; 15. a second reduction motor; 16. an ultraviolet detector; 17. an inspection inlet; 18. an electric cabinet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, in this embodiment: the utility model provides a data analysis device for digital PCR fluorescence detector that analysis efficiency is high which characterized in that: comprises a base 1, a shell 2 is arranged above the base 1, a standard liquid placing port 3 is arranged on one end face of the shell 2, a standard liquid disc 4 is arranged in the standard liquid placing port 3, a support 5 is arranged above the standard liquid disc 4, an air pump 6 is arranged at the upper end of the support 5, a screw rod lifting motor 7(SWL1 model) is arranged below the air pump 6, a lifting plate 8 is arranged below the screw rod lifting motor 7, a liquid taking test tube 9 is arranged on the lifting plate 8, a first slide rail 10 is arranged at the lower end of the support 5, a first speed reducing motor 11(DMK36-3650) is arranged on the first slide rail 10, a second slide rail 12 is arranged at one side of the first slide rail 10, a sample collecting plate 13 is arranged on the second slide rail 12, a sample collecting pit 14 is arranged on the sample collecting plate 13, a second speed reducing motor 15(DMK36-3650) is arranged on the second slide rail 12, an ultraviolet detector 16 is arranged at one side of the sample collecting plate 13, and an end face of the ultraviolet detector 16 close to the sample receiving plate 13 is provided with a detection inlet 17, and the upper end of the shell 2 is provided with an electric cabinet 18.
In the embodiment, the outer diameter of the standard liquid disc 4 is matched with the inner diameter of the standard liquid placing port 3, and the standard liquid disc 4 is connected with the standard liquid placing port 3 in a sliding way through a sliding chute, the bracket 5 is connected with the first sliding rail 10 in a sliding way through a pulley, the sample receiving plate 13 is connected with the second slide rail 12 in a sliding way through a pulley, the screw rod lifting motor 7 is fixedly connected with the lifting plate 8 through a screw rod, and the lifting plate 8 is connected with the bracket 5 in a sliding way through a sliding chute, the horizontal height of the second slide rail 12 is slightly higher than that of the first slide rail 10, the horizontal height of the bottom end of the liquid taking test tubes 9 is slightly higher than that of the sample collecting plate 13, the number of the liquid taking test tubes 9 is fourteen groups, and fourteen groups of liquid taking test tubes 9 are divided into two rows and fixed on the lifting plate 8 at equal intervals, the liquid taking test tubes 9 are communicated with the air pump 6 through air pipes, and the positions of the liquid taking test tubes 9 correspond to the positions of the sample collecting pits 14.
The utility model discloses a theory of operation and use flow: when the utility model is used, firstly, standard liquid which needs to be detected and analyzed is placed in a standard liquid disc 4, the standard liquid disc 4 is placed below a support 5 through a standard liquid placing port 3, then an electric cabinet 18 is operated, a screw rod lifting motor 7 works to enable a lifting plate 8 to descend, meanwhile, an air pump 6 starts working, the air pump 6 compresses air, suction force is generated in a liquid taking test tube 9 through an air pipe, the standard liquid in the standard liquid disc 4 which is correspondingly placed at the bottom of the liquid taking test tube 9 is sucked, then the screw rod lifting motor 7 drives the lifting plate 8 to ascend, a first speed reducing motor 11 starts working and drives the support 5 to slide, so that the support 5 is positioned at the upper part of a sample receiving plate 13, the liquid taking test tube 9 corresponds to the sample receiving plate 13, then the screw rod lifting motor 7 drives the lifting plate 8 to descend, meanwhile, the air pump 6 compresses air, and enables the standard liquid sucked in the liquid taking test tube 9 to drop into a plurality of sample receiving nests 14 on the sample receiving plate 13 through the air pipe, then, the second speed reducing motor 15 is operated through the electric cabinet 18 to work, the sample receiving plate 13 is driven to enter the ultraviolet detector 16 through the inspection inlet 17 to perform data analysis, and if the reaction group number of the standard solution to be detected needs to be changed, the quantity of the liquid taking test tubes 9 can be changed by controlling the air pump 6 through controlling the electric cabinet 18.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. The utility model provides a data analysis device for digital PCR fluorescence detector that analysis efficiency is high which characterized in that: comprises a base (1), a shell (2) is arranged above the base (1), a standard liquid placing port (3) is arranged on one end face of the shell (2), a standard liquid disc (4) is arranged in the standard liquid placing port (3), a support (5) is arranged above the standard liquid disc (4), an air pump (6) is arranged at the upper end of the support (5), a lead screw lifting motor (7) is arranged below the air pump (6), a lifting plate (8) is arranged below the lead screw lifting motor (7), a liquid taking test tube (9) is arranged on the lifting plate (8), a first slide rail (10) is arranged at the lower end of the support (5), a first speed reducing motor (11) is arranged on the first slide rail (10), a second slide rail (12) is arranged on one side of the first slide rail (10), and a sample collecting plate (13) is arranged on the second slide rail (12), the sample collecting device is characterized in that a sample collecting nest (14) is arranged on the sample collecting plate (13), a second speed reducing motor (15) is arranged on the second sliding rail (12), an ultraviolet detector (16) is arranged on one side of the sample collecting plate (13), a detection inlet (17) is formed in one end face, close to the sample collecting plate (13), of the ultraviolet detector (16), and an electric cabinet (18) is arranged at the upper end of the shell (2).
2. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the outer diameter of the standard liquid disc (4) is matched with the inner diameter of the standard liquid placing port (3), and the standard liquid disc (4) is in sliding connection with the standard liquid placing port (3) through a sliding groove.
3. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the support (5) is connected with the first sliding rail (10) in a sliding mode through a pulley, and the sample collecting plate (13) is connected with the second sliding rail (12) in a sliding mode through the pulley.
4. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the screw rod lifting motor (7) is fixedly connected with the lifting plate (8) through a screw rod, and the lifting plate (8) is connected with the support (5) in a sliding mode through a sliding groove.
5. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the horizontal height of the second slide rail (12) is slightly higher than that of the first slide rail (10), and the horizontal height of the bottom end of the liquid taking test tube (9) is slightly higher than that of the sample collecting plate (13).
6. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the number of the liquid taking test tubes (9) is fourteen groups, and the fourteen groups of the liquid taking test tubes (9) are fixed on the lifting plate (8) in two rows at equal intervals.
7. The data analysis device for a digital PCR fluorescence detector having high analysis efficiency according to claim 1, wherein: the liquid taking test tube (9) is communicated with the air pump (6) through an air pipe, and the position of the liquid taking test tube (9) corresponds to the position of the sample collecting nest (14).
CN202022306358.0U 2020-10-15 2020-10-15 Data analysis device for digital PCR fluorescence detector with high analysis efficiency Active CN214361382U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022306358.0U CN214361382U (en) 2020-10-15 2020-10-15 Data analysis device for digital PCR fluorescence detector with high analysis efficiency

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022306358.0U CN214361382U (en) 2020-10-15 2020-10-15 Data analysis device for digital PCR fluorescence detector with high analysis efficiency

Publications (1)

Publication Number Publication Date
CN214361382U true CN214361382U (en) 2021-10-08

Family

ID=77968260

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022306358.0U Active CN214361382U (en) 2020-10-15 2020-10-15 Data analysis device for digital PCR fluorescence detector with high analysis efficiency

Country Status (1)

Country Link
CN (1) CN214361382U (en)

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