CN208026632U - Measure the device of plasmodium content - Google Patents
Measure the device of plasmodium content Download PDFInfo
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- CN208026632U CN208026632U CN201820184127.7U CN201820184127U CN208026632U CN 208026632 U CN208026632 U CN 208026632U CN 201820184127 U CN201820184127 U CN 201820184127U CN 208026632 U CN208026632 U CN 208026632U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The utility model is related to a kind of devices measuring plasmodium content.The device includes：Filter assemblies, for removing the leucocyte in sample to be tested；Processing component includes reaction tank and dosing tank, dosing tank is connected to reaction tank by first pipe, dosing tank is for loading cell treatment fluid, and cell treatment fluid can be flowed by first pipe in reaction tank, reaction tank is connected to filter assemblies, and filtered sample to be tested is reacted with cell treatment fluid to form cell suspension in reaction tank；Separation assembly is connected to the reaction tank, and separation assembly is for being separated by solid-liquid separation cell suspension to obtain sediment, if sample to be tested contains the cell for carrying plasmodium, the cell for carrying plasmodium is uncracked and be separated by solid-liquid separation and be placed in sediment；And detection components obtain the content of plasmodium in sample to be tested for detecting sediment.Above-mentioned apparatus can measure the content of plasmodium without extracting malarial pigment, simple in structure, easy to operate, and accuracy is high.
Description
Technical field
The utility model is related to medical instruments fields, more particularly to a kind of device measuring plasmodium content.
Background technology
Plasmodium is the pathogen of malaria, is a kind of obligate intracellular parasite causing malaria.Plasmodium, which passes through, presses mosquito bite
Or input tape plasmodium person blood and invade in the red blood cell of human or animal, digest hemoglobin and generate the insoluble malaria of black
The problems such as pigment, induction malaria or anaemia.If to plasmodium is carried in the blood product of patient's input, not only bad for patient
Disease treatment, malaria can be also induced, therefore, accurate using before blood product, needing to carry out its plasmodium content
It measures.Meanwhile the worm blood rate of plasmodium under culture conditions is about 0.1%~5%, positioning to plasmodium and quantitative also to malaria
The exploitation of protozoon related basic research and drug is most important.
Currently, mainly carrying out the detection of plasmodium by the methods of smear staining Microscopical Method For Detection or malarial pigment detection method.Wherein,
Smear staining Microscopical Method For Detection is cumbersome, and contains a large amount of leucocyte and normal red blood cell, these cells pair in blood product
There are larger interference for the detection of the cell of carrying plasmodium, and then cause accuracy in detection relatively low.And malarial pigment detection method needs
By all cell crackings including carrying the cell of plasmodium and not carrying the cell of plasmodium, and malarial pigment is extracted
Out, cumbersome, extraction operation be easy to cause malarial pigment and is destroyed and influences subsequent detection, meanwhile, malarial pigment detection method
It is accurate relatively low, when the infective dose of plasmodium in cell to be measured is less or malarial pigment accumulation is less, can not effectively detect.
Utility model content
Based on this, provide a kind of without extracting cruel pigment and the higher device for measuring plasmodium content of accuracy.
A kind of device measuring plasmodium content, including：
Filter assemblies remove the leucocyte in the sample to be tested for filtering sample to be tested；
Processing component, including reaction tank and dosing tank, the dosing tank are connected to the reaction tank by first pipe, institute
Dosing tank is stated for loading cell treatment fluid, and the cell treatment fluid can flow into the reaction tank by the first pipe
In, the reaction tank is connected to the filter assemblies, the filtered sample to be tested in the reaction tank with the cell
Treatment fluid is reacted to form cell suspension；
Separation assembly is connected to the reaction tank, and the separation assembly is for being separated by solid-liquid separation the cell suspension to obtain
Sediment, if the sample to be tested contains the cell for carrying plasmodium, it is described carry plasmodium cell is uncracked and solid-liquid
Separation is placed in the sediment；And
Detection components obtain the content of plasmodium in the sample to be tested for detecting the sediment.
The device of said determination plasmodium content includes filter assemblies, processing component, separation assembly and detection components, mistake
Filter component can remove the leucocyte in sample to be tested, the interference to avoid leucocyte to plasmodium assay；Processing component
Including reaction tank and dosing tank, reaction tank is connected to filter assemblies, so that filtered sample to be tested can enter in reaction tank
It is reacted with cell treatment fluid, dosing tank is connected to reaction tank, and cell treatment fluid can be directly passed through into reaction tank so that filtering
Sample to be tested afterwards can directly be reacted with cell treatment fluid in reaction tank, make the cell cracking for not carrying plasmodium, and be taken
Cell with plasmodium is uncracked, and then forms cell suspension；After separation assembly through being connected to reaction tank is separated by solid-liquid separation, split
The cell of solution is located in supernatant, and the cell for carrying plasmodium is located in sediment, to will carry plasmodium cell enrichment and
Separation avoids interference of a large amount of normal cell to plasmodium assay, and then ensures the accuracy of testing result.Above-mentioned dress
The content of plasmodium can be measured without extracting malarial pigment by setting, simple in structure, easy to operate, and accuracy is high.In addition, above-mentioned dress
The content for the plasmodium that can be used in measuring blood product is set, so that the blood product can be applied to scientific research or disease
In treatment, plasmodium related basic research and drug development are also helped.
The filter assemblies include filter and pressurizer in one of the embodiments, the reaction tank and the mistake
Filter is connected to, and the pressurizer is connect with the filter, and the pressurizer is used to provide power to the filter.
The filter includes filtering body and filter house in one of the embodiments, and the filtering body is hollow
Structure, the filtering body are equipped with adding mouth and outlet, and the pressurizer is connect with the adding mouth, the outlet and institute
The setting of adding mouth interval is stated, the reaction tank is connected to the outlet, so that the reaction tank is connect with the filter assemblies,
The filter house is contained in the filtering body.
The filter assemblies further include liquid storage tank in one of the embodiments, after the liquid storage tank is used for stored filter
The sample to be tested, and the liquid storage tank is connected to the filtering body, and the reaction tank is connected to the liquid storage tank so that
The reaction tank is connected to the filtering body.
The filtering body is equipped with support plate in one of the embodiments, and the support plate is contained in the filtering originally
In body, the support plate is equipped with through-hole, and the filter house is set in the support plate, and filter house masking is described logical
Hole.
Blender is equipped in the reaction tank in one of the embodiments, the blender is for making filtered institute
State sample to be tested and the cell treatment fluid mixing.
The separation assembly includes separator in one of the embodiments, and the separator is described for being separated by solid-liquid separation
Cell suspension, the separator are equipped with inlet, and the reaction tank is equipped with discharge port, and the inlet connects with the discharge port
It is logical, so that the separator is connected to the reaction tank.
The detection components include Raman detector in one of the embodiments, and the Raman detector is for detecting
The sediment, to obtain the corresponding Raman spectrum of the sediment.
The detection components further include analysis processor in one of the embodiments, and the analysis processor is equipped with and connects
Receiving end, the Raman detector are equipped with output end, and the receiving terminal is connect with the output end, so that the analysis processor energy
It is enough that the Raman spectrum is analyzed, if the Raman spectrum is 1368cm in Raman shift-1~1378cm-1There are Ramans
Peak then contains plasmodium in the sample to be tested.
The detection components further include sampler in one of the embodiments, the sampler include handpiece and with
The sampling part of the handpiece connection, the sampler are used for after being sampled in the separation assembly into the Raman detector
Sample-adding.
Description of the drawings
Fig. 1 is the structural schematic diagram of the device of the measurement plasmodium content of an embodiment；
Fig. 2 is that device shown in FIG. 1 omits the structural schematic diagram after detection components；
Fig. 3 is that device shown in FIG. 1 omits the structural schematic diagram after detection components；
Fig. 4 is partial sectional view of the filter along A-A' lines of device shown in Fig. 2；
Fig. 5 is the structural schematic diagram of the sample injector of device shown in FIG. 1；
Fig. 6 is the partial sectional view of the filter of another embodiment.
Specific implementation mode
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms
It realizes, however it is not limited to embodiment described herein.Make to the utility model on the contrary, purpose of providing these embodiments is
The understanding of disclosure is more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model
The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.
As shown in Figure 1, the device 10 of the measurement plasmodium content of an embodiment, including filter assemblies 100, processing component
200, separation assembly 300 and detection components 400.
Also referring to Fig. 2, filter assemblies 100 include filter 110, pressurizer 120 and liquid storage tank 130.Filter 110
For filtering sample to be tested to remove the leucocyte in sample to be tested.Wherein, sample to be tested is experiment blood product or medical
Blood product.Certainly, it should be noted that sample to be tested is not limited to the above-mentioned sample pointed out, can also other need measure malaria
The sample of protozoon content, such as cultivate the culture of plasmodium.
Also referring to Fig. 2, Fig. 3 and Fig. 4, filter 110 includes filtering body 111 and filter house 113.Filtering body
111 be the shell of filter 110.In the shown embodiment, filtering body 111 is stainless steel cylinder, and the substantially cylindrical bodily form.
Certainly, it should be noted that filtering body 111 is not limited to stainless steel cylinder, or glass infuser.Filtering body 111 includes the
One shell 1110, second shell 1111, bolster 1112 and fixing piece 1113.In the shown embodiment, first shell 1110
One end of the substantially cylindrical bodily form, first shell 1110 is equipped with opening, and the edge being open is equipped with the first flange 1114.
First shell 1110 is equipped with adding mouth 1115, and first shell 1110 is equipped with sealing element (not shown), and sealing element is used for
Close adding mouth 1115.In the shown embodiment, adding mouth 1115 is opened in first shell 1110 far from the first flange 1114
One end, and adding mouth 1115 and the opening of first shell 1110 are oppositely arranged.Further, first shell 1110 is equipped with air inlet
Mouth 1116, air inlet 1116 are arranged at intervals at the one end of first shell 1110 far from the first flange 1114 with adding mouth 1115, and
Air inlet 1116 is opposite with the opening of first shell 1110.
Second shell 1111 is oppositely arranged with first shell 1110, and second shell 1111 is affixed and close with first shell 1110
Envelope connection.In the shown embodiment, the 1111 substantially cylindrical bodily form of second shell.One end of second shell 1111 is equipped with and opens
Mouthful, the opening of second shell 1111 is opposite with the opening of first shell 1110, and so that second shell 1111 and first shell
1110 communicate.The edge of the opening of second shell 1111 is equipped with the second flange 1117.
Second shell 1111 is equipped with support plate 1118, and support plate 1118 is contained in second shell 1111 and masking second shell
The opening of body 1111.In the shown embodiment, the substantially disc of support plate 1118, and the outer diameter of support plate 1118 and second
The internal diameter of shell 1111 is suitable, and support plate 1118 is an integral molding structure with second shell 1111, so that support plate 1118 is just
It is good to be fixedly contained in second shell 1111.Certainly, it should be noted that support plate 1118 is not limited to second shell 1111
It is an integral molding structure, or other fixed connection modes, such as support plate 1118 can be welded in the interior of second shell 1111
On wall.Further, support plate 1118 is equipped with through-hole (not shown), and the through-hole of support plate 1118 runs through support plate 1118, and props up
The through-hole of fagging 1118 is opposite with the opening of second shell 1111 and communicates.In the shown embodiment, support plate 1118 is logical
Hole is multiple, the through-hole interval of multiple support plates 1118 and is uniformly distributed.
Further, second shell 1111 is equipped with outlet 1119.In the shown embodiment, second shell 1111 is separate
One end of first shell 1110 is equipped with outlet 1119, and outlet 1119 and the through-hole of support plate 1118 are oppositely arranged.
Bolster 1112 is for being tightly connected first shell 1110 and second shell 1111.In the shown embodiment, delay
Stamping 1112 is sealing ring.Bolster 1112 is between first shell 1110 and second shell 1111, and bolster 1112
Both sides can be abutted with the first flange 1114 with the second flange 1117 respectively, so that bolster 1112 is held on the first flange 1114
Between the second flange 1117, and then first shell 1110 is made to be tightly connected with second shell 1111.Certainly, it needs to illustrate
It is that bolster 1112 can also be directly fixed on the first flange 1114 close to the side of the second flange 1117.
Fixing piece 1113 is for being fixedly connected with first shell 1110 and second shell 1111.In the shown embodiment, Gu
Determine the substantially circular ring shape of part 1113.Fixing piece 1113 be equipped with annular groove 1120, fixing piece 1113 be sheathed on first shell 1110 with
Second shell 1111, the first flange 1114 and the second flange 1117 can be fixedly contained in annular groove 1120.Specifically,
First flange 1114 and the second flange 1117 can be held in fixing piece 1113, so that bolster 1112 is closely held on
Between one flange 1114 and the second flange 1117, and then keeps first shell 1110 affixed with second shell 1111 and be tightly connected.
Filter house 113 is filter medium, for removing the leucocyte in sample to be tested.Filter house 113 is contained in filtering originally
In body 111.In the shown embodiment, filter house 113 is filter membrane, and the aperture of filter house 113 is 10 μm~20 μm.Further
Ground, filter house 113 are contained in second shell 1111, and on side of the support plate 1118 far from first shell 1110, and
The filter house 113 covers the through-hole of support plate 1118.Certainly, it should be noted that filter house 113 is not limited to filter membrane,
It can be filter core, such as can be that fiber fills filter core.
Pressurizer 120 is connect with filter 110, and pressurizer 120 can give filter 110 to provide power.It is real in diagram
It applies in mode, pressurizer 120 is gas compressor, can be that filter 110 provides compressed gas.Further, pressurizer 120
Equipped with connecting tube 121, compressed air is discharged from connecting tube 121, the one end of connecting tube 121 far from pressurizer 120 can with into
Gas port 1116 is connected to and is tightly connected, in order to be passed through compressed gas into filter 110.
Liquid storage tank 130 is used for the sample to be tested after stored filter.Liquid storage tank 130 includes tank body 131, connecting tube 132, stirring
Device 133 and refrigerator 134.Tank body 131 is the main body of liquid storage tank 130.In the shown embodiment, tank body 131 is substantially cylindrical
The bodily form.Tank body 131 is equipped at intervals with inlet 1311 and liquid outlet 1312.In the shown embodiment, inlet 1311 and go out liquid
Mouth 1312 is respectively arranged on the both ends of tank body 131.
Connecting tube 132 is for being connected to liquid storage tank 130 and filter 110.Specifically, one end of connecting tube 132 and outlet
1119 connections, the other end are connected to inlet 1311.Further, connecting tube 132 is equipped with stream close to one end of inlet 1311
Amount controller (not shown), the flow velocity for the liquid for flowing into tank body 131 is monitored and controlled.
Blender 133 is used to keep the uniform of the filtered sample to be tested in tank body 131, in order to avoid taking in sample to be tested
Cell settlement with plasmodium influences the measurement of plasmodium content.Blender 133 include stirring rod 1331, stir plate portion 1332 and
Motor 1333.Stirring rod 1331 is contained in tank body 131, and one end of stirring rod 1331 is rotatably connected at and is fixed on tank body
131 close to one end of inlet 1311.In the shown embodiment, stirring rod 1331 is retractable structure.
Mixing part 1332 is contained in tank body 131, and is fixed on the other end of stirring rod 1331, so that 1332 energy of mixing part
It is enough to be rotated relative to tank body 131 with stirring rod 1331.Wherein, mixing part 1332 is straight leaf disc turbine slurry, straight leaf unlatching turbine
Slurry, oblique leaf open turbine slurry or curved leaf opens turbine slurry.In the shown embodiment, there are two mixing part 1332 is total to, two are stirred
Mix the other end that portion 1332 is arranged at intervals at stirring rod 1331.Certainly, it should be noted that the quantity of mixing part 1332 is not limited to
Two, or one, can also be three, be configured according to actual conditions.
Motor 1333 is for driving stirring rod 1331.Motor 1333 is located at the outside of tank body 131, and affixed with tank body 131.
Further, motor 1333 is set to the one end of stirring rod 1331 far from mixing part 1332, to drive stirring rod 1331 to rotate, into
And mixing part 1332 is driven to rotate.
Refrigerator 134 is waited for for making filtered sample to be tested in tank body 131 keep lower temperature to avoid filtered
This microbiological contamination of test sample or denaturation, and then influence the measurement of follow-up plasmodium content.Refrigerator 134 is condenser pipe, and refrigerator 134 is worn
And be partially housed in tank body 131, simultaneously circulating water can be passed through into refrigerator 134, so that the liquid in tank body 131 is kept
Lower temperature.
Processing component 200 includes reaction tank 210, dosing tank 220 and first pipe 230.Reaction tank 210 is processing component
200 main body, for reacting filtered sample to be tested to form cell suspension.Reaction tank 210 includes retort 211, connection
Pipe 212, blender 213, temperature controller 214 and main controller (not shown).Retort 211 is the main body of reaction tank 210.Scheming
Show in embodiment, the 211 substantially cylindrical bodily form of retort.Retort 211 is equipped at intervals with feed inlet 2111 and discharge port 2112.
In the shown embodiment, feed inlet 2111 and discharge port 2112 are respectively arranged on the both ends of retort 211.Further, it reacts
Tank 211 is equipped with dosing mouth 2113, and the dosing mouth 2113 of retort 211 is set to the top of retort.
Connecting tube 212 is for connected reaction pond 210 and liquid storage tank 130.Specifically, one end of connecting tube 212 and liquid outlet
Connection, the other end are connected to feed inlet 2111.Further, connecting tube 212 is equipped with flow control close to one end of feed inlet 2111
Device (not shown) processed flows into the flow velocity in retort 211 with the liquid being monitored and controlled in liquid storage tank 130.
Blender 213 is used for the liquid in mixing retort 211.Blender 213 includes stirring rod 2131, stirs plate portion 2132
And motor 2133.Stirring rod 2131 is contained in retort 211, and one end of stirring rod 2131 is rotatably connected at and is fixed on
One end of retort 211.In the shown embodiment, stirring rod 2131 is retractable structure.
It stirs plate portion 2132 to be contained in retort 211, and is fixed on the other end of stirring rod 2131, so as to stir plate portion 2132
It can be rotated relative to retort 211 with stirring rod 2131.Wherein, it is straight leaf disc turbine slurry, the unlatching of straight leaf to stir plate portion 2132
Turbine slurry, oblique leaf open turbine slurry or curved leaf opens turbine slurry.Motor 2133 is for driving stirring rod 2131.In diagram embodiment party
In formula, mixing part 2132 altogether there are two, two mixing parts 2132 are arranged at intervals at the other end of stirring rod 2131.Certainly, it needs
Illustrate, the quantity of mixing part 2132 is not limited to two, or one, can also be three, according to actual conditions into
Row setting.
Motor 2133 is fixed on retort 211 close to one end of feed inlet 2111, and positioned at the outside of retort 211.Electricity
Machine 2133 is connect with the one end of stirring rod 2131 far from mixing part 2132, to drive stirring rod 2131 to rotate, and then is driven and is stirred plate
Portion 2132 rotates.
Temperature controller 214 is used to control the temperature of liquid in retort 211.Temperature controller 214 includes heating part
2141, condensation part 2142 and sensor (not shown).In the shown embodiment, heating part 2141 is heating jacket, heating part
2141 are fixedly sheathed on the peripheral surface of retort 211, in order to be heated to retort 211.Condensation part 2142 is condenser pipe, cold
Solidifying portion 2142 wears retort 211, and is partially housed in retort 211.It can be passed through circulating water into condensation part 2142,
To reduce the temperature of liquid in retort 211.Sensor wears and is contained in retort 211, in order to sensor with react
Liquid contacts in tank 211, to incude the temperature of liquid in retort 211.
Main controller is electrically connected with motor 2133, sensor, can control blender 213 according to the program of pre-selection setting
Rotating speed, also can by control condensation part 2142 flow velocity and heating part 2141 power by control liquid in retort 211
Temperature, additionally it is possible to control the time of reaction.
Dosing tank 220 is for loading cell treatment fluid.Wherein, polyoxyethylene ether and 4- hydroxyl piperazines are contained in cell treatment fluid
Piperazine ethanesulfonic acid, a concentration of 4.0g/L~6.0g/L of polyoxyethylene ether, a concentration of 4.0g/L of 4- hydroxyl piperazine ethanesulfonic acids~
5.5g/L.The cell treatment fluid can crack the cell for not carrying plasmodium, and the cell for carrying plasmodium is uncracked.Further
Ground, in cell treatment fluid also the potassium dihydrogen phosphate containing 0.2g/L~0.35g/L, 1.0g/L~2.0g/L disodium hydrogen phosphate,
The bovine serum albumin bletilla 0.5g/ of the sodium chloride of 6.5g/L~9.5g/L, the potassium chloride of 0.1g/L~0.5g/L, 4g/L~8g/L
The EDETATE SODIUM of L~1.0g/L.Certainly, it should be noted that cell treatment fluid be not limited to it is above-mentioned point out to be formulated, as long as can will
The erythrocyte splitting for not carrying plasmodium carries the red blood cell of plasmodium without cracking.
In the shown embodiment, the 220 substantially cylindrical bodily form of dosing tank.Dosing tank 220 is equipped at intervals with feeding opening 221
With medicine outlet 223.In the shown embodiment, feeding opening 221 is respectively equipped with the both ends of dosing tank 220, dosing with medicine outlet 223
The medicine outlet 223 of case 220 is connected to the dosing mouth 2113 of reaction tank 210, so that dosing tank 220 can be flowed into reaction tank 210
Enter cell treatment fluid.
First pipe 230 is for being connected to dosing tank 220 and reaction tank 210.In the shown embodiment, first pipe 230
One end be connected to the dosing mouth 2113 of retort 211, the other end is connected to the medicine outlet 223 of dosing tank 220, so that dosing tank
220 are connected to reaction tank 210.Cell treatment fluid in dosing tank 220 can be flowed by first pipe 230 in reaction tank 210,
And reacted with the filtered sample to be tested in reaction tank 210, to obtain cell suspension.Further, it is set in first pipe 230
There are flow controller (not shown), flow controller to be used to that the stream for flowing into the cell treatment fluid in reaction tank 210 to be monitored and controlled
Speed.
Separation assembly 300 is connected to reaction tank 210, for being separated by solid-liquid separation cell suspension to obtain sediment, if waiting for test sample
Product contain carry plasmodium cell, then carry plasmodium cell it is uncracked and be separated by solid-liquid separation be placed in sediment.Separation
Component 300 includes whizzer 310 and second pipe 320.Whizzer 310 is connected to reaction tank 210, for solid-liquid point
From cell suspension.
Whizzer 310 includes separator body 311, separator lid 312, motor (not shown) and control panel
(not shown).Separator body 311 is the main body of whizzer 310, and separator body 311 is internally provided with host cavity, and (figure is not
Show), host cavity is for accommodating cell suspension.Separator body 311 is equipped with the liquid outlet 313 communicated with host cavity, after centrifugation
Supernatant is flowed out from the liquid outlet 313 of separator body 311.Liquid outlet 313 is equipped with outlet valve (not shown), and outlet valve is for opening
Open or close liquid outlet 313.
Separator lid 312 is covered on separator body 311, so that whizzer 310 forms confined space.Separator
Lid 312 is equipped with the inlet 314 communicated with host cavity, and cell suspension flows into host cavity through inlet 314.In diagram embodiment party
In formula, inlet 314 is set to side of the separator lid 312 far from separator body 311.Inlet 314 is equipped with inlet valve (figure
Do not show), inlet valve is for being turned on and off inlet 314.Motor is used to provide power, to be separated by solid-liquid separation cell suspension.Control
Panel is used to control rotating speed and the time of whizzer 310.
Detection components 400 obtain the content of plasmodium in sample to be tested for detecting sediment.Detection components 400 include
Raman detector 410, sampler 420, analysis processor 430.Raman detector 410 is for detecting sediment, to be settled
The corresponding Raman spectrum of object.Raman detector 410 is equipped with output end (not shown), and output end is for exporting Raman spectrum.
Please refer to fig. 5, sampler 420 is used to add into Raman detector 410 after sampling in separation assembly 300
Sample.Sampler 420 includes the sampling part 422 that handpiece 421 and handpiece 421 connect.In the shown embodiment, handpiece
421 substantially bar shapeds.One end of handpiece 421 is equipped with connecting rod 423, and connecting rod 423 is hollow.The other end of handpiece 421
Equipped with driving portion 424, driving portion 424 can be relative to handpiece 421 to the direction movement closer or far from connecting rod 423.Scheming
Show in embodiment, sampling part 422 substantially cone shape, and is hollow.Sampling part 422 is sheathed on connecting rod 423 far from drive
The one end in dynamic portion 424, and communicated with connecting rod 423.It can enable driving portion 424 by applying active force to driving portion 424
Relative to handpiece 421 to the direction movement closer or far from connecting rod 423, and then suck the sample into sampling part 422 or by liquid
Body is discharged from sampling part 422.
Analysis processor 430 includes analysis module 431 and print module 432.Analysis module 431 be used for Raman spectrum into
Row analysis, if Raman spectrum is 1368cm in Raman shift-1~1378cm-1There are Raman peaks, then former containing malaria in sample to be tested
Worm.Analysis module 431 can also be 750cm by analyzing Raman spectrum in Raman shift in one of the embodiments,-1~
760cm-1With the presence or absence of Raman peaks, and then judge whether sediment is red blood cell.
Analysis module 431 is generally common computer, and corresponding function is completed by built-in specific configuration software.Analysis
Module 431 be equipped with receiving terminal (not shown), the receiving terminal of analysis module 431 be electrically connected with the output end of Raman detector 410 and
Communication connection, so that Raman spectrum can be passed in analysis module 431, so that analysis module 431 can be to Raman spectrum
It is analyzed.Print module 432 is used to print the result of Raman spectrum and analysis.Print module 432 is electrically connected with analysis module 431
It connects and communicates to connect, so that print module 432 can print the result of Raman spectrum and analysis.
One embodiment measurement plasmodium content device 10 use process is as follows：
Sample to be tested is added in filter 110, opens pressurizer 120 with to being passed through compressed air in filter 110, into
And sample to be tested is filtered to remove the leucocyte in sample to be tested, obtain filtered sample to be tested.Filtered sample to be tested
It is flowed into liquid storage tank 130 through connecting tube 132, it can be with turn on agitator 133 and refrigerator 134.
Filtered sample to be tested is flowed into through connecting tube 212 in reaction tank 210 from liquid storage tank 130, can adjust connecting tube
212 flow controller, the flow velocity that filtered sample to be tested flows into retort 211 is monitored and controlled.Meanwhile cell is handled
Liquid is flowed into through first pipe 230 in retort 211 from dosing tank 220, and turn on agitator 212 and temperature controller 214 immediately,
Temperature in rotating speed and retort 211 is controlled in suitable range, and controls cell treatment fluid and sample to be tested after filtering
Reaction time obtains cell suspension.
The cell being cleaved to obtain sediment is separated by solid-liquid separation in cell suspension inflow whizzer 310 to be located at
In supernatant, the cell for carrying plasmodium is located in sediment.With physiological saline or PBS buffer solution diluted settlement object, sampler is used
420 sediments and being added in Raman detector 410 for drawing after dilution are detected, and obtain Raman spectrum.Raman spectrum is passed
It transports in analysis module 431 and is analyzed, if Raman spectrum is 1368cm in Raman shift-1~1378cm-1There are Raman peaks,
Then contain plasmodium in sample to be tested.The result of Raman spectrum and analysis can also be printed by print module 432.
The device 10 of the measurement plasmodium content of one embodiment at least has the following advantages that：
(1) device 10 of said determination plasmodium content includes filter assemblies 100, processing component 200, separation assembly
300 and detection components 400, filter assemblies 100 can remove the leucocyte in sample to be tested, plasmodium is contained to avoid leucocyte
Measure fixed interference；Processing component 200 includes reaction tank 210 and dosing tank 220, and reaction tank 210 is connected to filter assemblies 100,
It is reacted with cell treatment fluid so that the filtered sample to be tested in filter assemblies 100 can enter in reaction tank 210, dosing tank
220 are connected to reaction tank 210 through first pipe 230, and cell treatment fluid can be directly passed through into reaction tank 210 so that filtering
Sample to be tested afterwards can directly be reacted with cell treatment fluid in reaction tank 210, make the cell cracking for not carrying plasmodium, and
The cell for carrying plasmodium is uncracked, and then forms cell suspension；300 solid-liquid of separation assembly through being connected to reaction tank 210 point
From rear, the cell being cleaved is located in supernatant, and the cell for carrying plasmodium is located in sediment, to carry the thin of plasmodium
Born of the same parents' enrichment and separation avoids interference of a large amount of normal cell to plasmodium assay, and then ensures the accurate of testing result
Degree.Above-mentioned apparatus 10 can measure the content of plasmodium without extracting malarial pigment, simple in structure, easy to operate, and accuracy is high,
High degree of automation.In addition, above-mentioned apparatus 10 can be used in measuring the content of the plasmodium of blood product, so that the blood product
It can be applied in scientific research or the treatment of disease, also help plasmodium related basic research and drug development.
(2) detection components 400 of the device 10 of said determination plasmodium content include Raman detector 410 and analyzing processing
Device 430, the high sensitivity of Raman detector 410 are conducive to the accuracy for improving plasmodium assay, meanwhile, analysis module
431 can not only be 1368cm by analyzing Raman spectrum in Raman shift-1~1378cm-1With the presence or absence of Raman peaks, with judgement
Contain plasmodium in sample to be tested, additionally it is possible to by analyze Raman spectrum Raman shift be 750cm-1~760cm-1Whether deposit
In Raman peaks, and then judge whether sediment is red blood cell.
(3) device 10 of said determination plasmodium content further includes print module 432, in order to print Raman spectrum and divide
Analyse result.
It is appreciated that first shell 1110 and the fixed connection mode of second shell 1111 are not limited to aforesaid way, first shell
1110 with second shell 1111 or be spirally connected or be clamped, while in the interconnecting piece of first shell 1110 and second shell 1111
Position setting washer, can equally realize the sealed connection of first shell 1110 and second shell 1111.Certainly, it needs to illustrate
It is first shell 1110 and second shell 1111 or integrated formed structure.
It is appreciated that adding mouth 1115 can also be set to the peripheral surface of first shell 1110, outlet 1119 can also
It is set to the peripheral surface of second shell 1111.
It is appreciated that adding mouth 1115 can be omitted with one in air inlet 1116.At this point it is possible to first by sample to be tested
After being added in one not omitted in middle adding mouth 1115 and air inlet 1116, then by one of connecting tube 121 far from pressurizer 120
End and adding mouth 1115 are connected to do not omitted in air inlet 1116 one, to give the offer power of filter 110.
Also referring to Fig. 6, in other embodiments, the structure of the structure of 110 ' of filter substantially with filter 110
It is roughly the same, the difference is that：Support plate is omitted, and 1119 ' of outlet is set to 1110 ' of first shell far from second shell
One end of 1111 ', and run through 1110 ' of first shell, 1115 ' of adding mouth and 1116 intervals ' of air inlet are set to 1111 ' of second shell
One end far from 1110 ' of first shell.
Further, 113 ' of filter house is filter core, and is cylindrical.In the shown embodiment, 113 ' packets of filter house
Include 1133 ' of 1131 ' of filter house ontology, 1132 ' of connecting tube and sealing element.1131 ' of filter house ontology is contained in 1110 ' of first shell
And 1111 ' of second shell, and and sealed connection affixed with 1110 ' of first shell, and 1131 ' of filter house ontology and first shell
There is gap in the inner wall of 1110 ', the inner wall of 1111 ' of second shell.
One end of 1132 ' of connecting tube is affixed with one end of 1131 ' of filter house ontology and is tightly connected, and 1132 ' of connecting tube is worn
And if being contained in 1119 ' of outlet.1132 ' of connecting tube and 1110 ' of first shell is affixed and is tightly connected, so that filter house ontology
1131 ' and 1110 ' of first shell is affixed and is tightly connected.Further, 1133 ' of sealing element is sheathed on 1132 ' of connecting tube, and close
1133 ' of sealing is held between the outer surface of 1131 ' of inner wall and filter portion ontology of 1110 ' of first shell, so that filter house ontology
1131 ' are tightly connected with 1110 ' of first shell.In the shown embodiment, 1133 ' of sealing element is sealing ring.
Sample to be tested enters 1131 ' of filter house ontology and 1110 ' of first shell, 1111 ' of second shell through 1115 ' of adding mouth
Inner wall in, and be passed through compression gap, under the action of compressed air, sample to be tested enters in 1131 ' of filter house ontology by mistake
Filter, filtered sample to be tested are flowed out through 1132 ' of connecting tube.The structure of 110 ' of above-mentioned filter is conducive to improve filter efficiency.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of device measuring plasmodium content, which is characterized in that including：
Filter assemblies remove the leucocyte in the sample to be tested for filtering sample to be tested；
Processing component, including reaction tank and dosing tank, the dosing tank are connected to the reaction tank by first pipe, described to add
Medicine-chest is for loading cell treatment fluid, and the cell treatment fluid can be flowed by the first pipe in the reaction tank,
The reaction tank is connected to the filter assemblies, and the filtered sample to be tested is handled in the reaction tank with the cell
Liquid is reacted to form cell suspension；
Separation assembly is connected to the reaction tank, and the separation assembly is for being separated by solid-liquid separation the cell suspension to be settled
Object, if the sample to be tested contains the cell for carrying plasmodium, the cell for carrying plasmodium is uncracked and is separated by solid-liquid separation
It is placed in the sediment；And
Detection components obtain the content of plasmodium in the sample to be tested for detecting the sediment.
2. the apparatus according to claim 1, which is characterized in that the filter assemblies include filter and pressurizer, described
Reaction tank is connected to the filter, and the pressurizer is connect with the filter, and the pressurizer is used for the filter
Power is provided.
3. the apparatus of claim 2, which is characterized in that the filter includes filtering body and filter house, described
Filtering body is hollow structure, and the filtering body is equipped with adding mouth and outlet, and the pressurizer is connect with the adding mouth,
The outlet is arranged with the adding mouth interval, and the reaction tank is connected to the outlet, so that the reaction tank and institute
Filter assemblies connection is stated, the filter house is contained in the filtering body.
4. device according to claim 3, which is characterized in that the filter assemblies further include liquid storage tank, the liquid storage tank
For the sample to be tested after stored filter, and the liquid storage tank is connected to the filtering body, the reaction tank with it is described
Liquid storage tank is connected to, so that the reaction tank is connected to the filtering body.
5. device according to claim 3, which is characterized in that the filtering body is equipped with support plate, and the support plate is received
It is dissolved in the filtering body, the support plate is equipped with through-hole, and the filter house is set in the support plate, and the filtering
Cover the through-hole in portion.
6. the apparatus according to claim 1, which is characterized in that be equipped with blender in the reaction tank, the blender is used
In making the filtered sample to be tested and the cell treatment fluid mixing.
7. the apparatus according to claim 1, which is characterized in that the separation assembly includes separator, and the separator is used
In being separated by solid-liquid separation the cell suspension, the separator is equipped with inlet, and the reaction tank is equipped with discharge port, the inlet with
The discharge port connection, so that the separator is connected to the reaction tank.
8. the apparatus according to claim 1, which is characterized in that the detection components include Raman detector, the Raman
Detector is for detecting the sediment, to obtain the corresponding Raman spectrum of the sediment.
9. device according to claim 8, which is characterized in that the detection components further include analysis processor, described point
It analyses processor and is equipped with receiving terminal, the Raman detector is equipped with output end, and the receiving terminal is connect with the output end, so that institute
The Raman spectrum can be analyzed by stating analysis processor, if the Raman spectrum is 1368cm in Raman shift-1~
1378cm-1There are Raman peaks, then contain plasmodium in the sample to be tested.
10. device according to claim 8, which is characterized in that the detection components further include sampler, the sampler
The sampling part being connect including handpiece and with the handpiece, the sampler are used for after being sampled in the separation assembly to institute
It states in Raman detector and is loaded.
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| CN201820184127.7U CN208026632U (en) | 2018-02-02 | 2018-02-02 | Measure the device of plasmodium content |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820184127.7U CN208026632U (en) | 2018-02-02 | 2018-02-02 | Measure the device of plasmodium content |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110736732A (en) * | 2019-12-09 | 2020-01-31 | 南阳理工学院 | Method and device for measuring body fluid drug concentration based on Raman spectrum |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110736732A (en) * | 2019-12-09 | 2020-01-31 | 南阳理工学院 | Method and device for measuring body fluid drug concentration based on Raman spectrum |
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