CN110736732A - Method and device for measuring body fluid drug concentration based on Raman spectrum - Google Patents
Method and device for measuring body fluid drug concentration based on Raman spectrum Download PDFInfo
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Abstract
The invention relates to a method and a device for measuring body fluid drug concentration based on Raman spectroscopy, which comprises the following steps of extracting body fluid, namely breaking red blood cells, placing the broken red blood cells into a centrifuge for centrifugal treatment, extracting supernatant of the centrifuged red blood cells, vibrating or swinging or rotating the supernatant collected in the third step, adding a nano-gold or nano-silver solution into the supernatant for uniform mixing, carrying out centrifugal concentration, sampling liquid in the fourth step, carrying out transmission Raman spectroscopy detection on the obtained sample to obtain a drug molecule surface enhanced Raman spectrogram, and obtaining the drug content in blood through model calculation.
Description
Technical Field
The invention relates to the technical field of body fluid drug concentration measurement, in particular to a body fluid drug concentration measurement method and a body fluid drug concentration detection device based on Raman spectroscopy.
Background
It is known that the amount of meal varies significantly among people and is not always proportional to body weight and body surface area. The same is true for the administration of standard doses, some patients may be under-dosed due to too rapid metabolism and some patients may be over-metabolized, resulting in substantial overdose and toxic side effects. If the concentration of the drug in blood is high or low, the curative effect is influenced, and the drug resistance of bacteria can be induced.
Therefore, monitoring of blood concentration is necessary, especially for drugs with narrow safety range and strong toxic and side effects, drugs with nonlinear pharmacokinetic properties, drugs with in vivo elimination rate constant dependent on dosage, drugs for patients with diseases of kidney, liver, heart, stomach and intestinal tract, drugs which need to be used for a long time, and drugs which are used in combination to cause the change of absorption, distribution or metabolism of the drugs due to the interaction of the drugs.
At present, phases are not accurate and feasible methods for detecting the content of the medicine in blood extracted after the medicine is taken by a human, so that the concentration content of the medicine in the blood cannot be accurately detected during detection, the medicine absorption rate of the human after the medicine is taken cannot be fully determined, and the risk of side effects is easily caused due to the fact that people with different physiques are not accurate enough during medicine taking.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the invention aims to provide body fluid drug concentration measuring methods and detecting devices based on raman spectroscopy, which can accurately measure the drug concentration in the body fluid of a human body after taking the drug.
In order to achieve the purpose, the invention adopts the following technical scheme, and the method for measuring the body fluid drug concentration based on the Raman spectrum is carried out according to the following method:
, extracting body fluid, namely extracting blood of a patient taking the medicine in a sterile environment, and diluting the extracted blood;
step two: and (3) red blood cell crushing treatment: placing the diluted blood into an ultrasonic power of 400-500W for acting for 25-30 min to carry out erythrocyte crushing treatment;
step three: placing the crushed red blood cells into a centrifuge for centrifugal treatment, and then extracting supernatant of the red blood cells after centrifugal treatment;
step four: shaking or swinging or rotating the supernatant collected in the third step, adding a nano gold or nano silver solution into the supernatant, uniformly mixing, and then carrying out centrifugal concentration;
step five: and C, sampling the liquid in the fourth step, carrying out transmission Raman spectrum detection on the obtained sample to obtain a drug molecule surface enhanced Raman spectrogram, and calculating through a model to obtain the drug content in the blood.
And in the second step, after the red blood cells are crushed, filtering the crushed red blood cells by a filter membrane.
The extracted blood was diluted with PBS buffer in step .
Diluting the extracted blood in step by using double distilled water with the volume of 1-2 times;
the third step is carried out according to the following method: adding anhydrous ethanol and chloroform into the crushed erythrocyte liquid sequentially, stirring while adding, standing for 30min after stirring, putting the mixed liquid into a centrifuge for centrifugation, and collecting the centrifuged supernatant; and then the ethanol and the chloroform are removed by a nitrogen blowing instrument.
The PBS buffer solution consists of the following substances in parts by weight:
8-12% of sodium dihydrogen phosphate, 5-10% of disodium hydrogen phosphate, 1-3% of potassium chloride, 0.5-1.5% of pancreatin and the balance of water.
Detection device of body fluid drug concentration based on raman spectroscopy includes:
an ultrasonic cell disruption and dispersion machine for disrupting red blood cells in the extracted blood;
the centrifugal separation device is used for injecting liquid after being crushed by the ultrasonic cell crushing and dispersing machine into the centrifugal separation device, and the centrifugal separation device is used for carrying out centrifugal separation on the crushed red blood cell liquid and separating out supernatant;
the mixed oscillation device is used for mixing and oscillating the supernatant separated by the centrifugal separation device with the nano gold or nano silver solution entering the mixed oscillation device;
a centrifugal concentrator, which extracts the liquid mixed by the mixing and oscillating device and adds the liquid into the centrifugal concentrator for centrifugal concentration treatment;
and the Raman spectrometer samples the liquid centrifugally concentrated by the centrifugal concentrator, and places the liquid into the Raman spectrometer for transmission Raman spectrum detection to obtain the concentration of the medicine in the blood.
The centrifugal separation device comprises a centrifugal outer barrel and a centrifugal inner barrel, wherein a plurality of centrifugal holes are formed in the centrifugal inner barrel in the circumferential direction, an outer barrel sealing cover is fixedly connected to the upper end of the centrifugal outer barrel, an inner barrel sealing cover is fixedly connected to the upper end of the centrifugal inner barrel, a liquid inlet pipe is fixedly connected to the inner barrel sealing cover, the upper end of the liquid inlet pipe penetrates through the outer barrel sealing cover to extend outwards, and the outer wall of the liquid inlet pipe is fixedly connected with the outer barrel sealing cover through a bearing; the liquid inlet pipe is provided with a one-way valve;
an independent sealing installation cavity is formed in the bottom of the centrifugal outer barrel, a centrifugal motor is fixedly arranged in the independent sealing installation cavity, a rotating shaft of the centrifugal motor penetrates through the top of the independent sealing installation cavity and is fixedly connected with the bottom of the centrifugal inner barrel, and the rotating shaft of the centrifugal motor, which extends out of the independent sealing installation cavity, is located in a sealing rotating barrel, wherein the bottom of the centrifugal inner barrel is movably connected with the top of the independent sealing installation cavity;
a filtering membrane is arranged between the outer side surface of the independent seal installation cavity and the inner side surface of the centrifugal outer cylinder, and a supernatant liquid outlet pipe is communicated with the centrifugal outer cylinder at the lower part of the filtering membrane;
the mixed oscillation device comprises an outer sound insulation box body and an oscillation box body arranged in the outer sound insulation box body, wherein two sides of the oscillation box body are fixedly connected with the inner wall of the sound insulation box body through a plurality of horizontal springs, the bottom of the oscillation box body is fixedly connected with the bottom in the outer sound insulation box body through a plurality of vertical springs, and an oscillation driving device for driving the oscillation box body to oscillate up and down in the outer sound insulation box body is fixedly arranged at the bottom in the outer sound insulation box body;
the top of the vibration box body is communicated with a liquid adding hose, and the end part of the liquid adding hose penetrates through the top of the sound insulation outer box body and extends outwards; the bottom of the vibration box body is communicated with a mixed liquid outlet hose, and the end part of the mixed liquid outlet hose penetrates through the side wall of the lower part of the sound insulation outer box body and extends outwards; the upper portions of the sound-insulation outer box body and the vibration box body are respectively provided with an outer liquid inlet and an inner liquid inlet, and the outer liquid inlet and the inner liquid inlet are communicated through a hose connecting assembly.
Vibrate drive arrangement including fixed establish driving motor, the fixed connection in the outer box that gives sound insulation in vibrate many perpendicular decurrent ejector pins of box, with driving motor's pivot fixed connection's drive shaft, the both ends of drive shaft and the fixed hub connection seat swing joint who is equipped with in outer box bottom gives sound insulation, be equipped with a plurality of cams in the drive shaft, and the circumference face of every cams contacts with the lower extreme of ejector pin.
The method has the advantages that the method can accurately measure the blood concentration of the human body after the medicine is taken, makes contribution to the absorption of different human bodies to the medicine, ensures that different people take more accurate medicine, protects patients from steps, prevents abuse of the medicine, and effectively protects the environment.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the centrifugal separator of the present invention;
FIG. 3 is a schematic structural diagram of a hybrid oscillator device according to the present invention;
fig. 4 is a schematic structural view of the sealed rotary cylinder of the present invention.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Thus, a feature defined as "", "second" may explicitly or implicitly include or more such features, and in the description of the invention, "plurality" means two or more unless otherwise indicated.
Example 1
The method for measuring the body fluid drug concentration based on the Raman spectrum is carried out according to the following method:
and , body fluid extraction, namely extracting blood of a patient taking the medicine in a sterile environment, diluting the extracted blood, extracting the blood of the patient in the sterile environment to ensure that the collected blood is not polluted by bacteria, and diluting the blood by adopting a PBS (phosphate buffer solution) when diluting the blood, wherein the PBS comprises the following substances in parts by weight:
8-12% of sodium dihydrogen phosphate, 5-10% of disodium hydrogen phosphate, 1-3% of potassium chloride, 0.5-1.5% of pancreatin and the balance of water, wherein the PBS buffer solution can be used for fully diluting blood;
step two: and (3) red blood cell crushing treatment: placing the diluted blood into an ultrasonic power of 400-500W for acting for 25-30 min to carry out erythrocyte crushing treatment; after the red blood cells are crushed, filtering the crushed red blood cells through a filtering membrane to ensure that the crushed red blood cells are effectively separated;
step three: placing the crushed red blood cells into a centrifuge for centrifugal treatment, and then extracting supernatant of the red blood cells after centrifugal treatment;
step four: shaking or swinging or rotating the supernatant collected in the third step, adding a nano gold or nano silver solution into the supernatant, uniformly mixing, and then carrying out centrifugal concentration;
step five: and C, sampling the liquid in the fourth step, carrying out transmission Raman spectrum detection on the obtained sample to obtain a drug molecule surface enhanced Raman spectrogram, and calculating through a model to obtain the drug content in the blood.
Example 2
, extracting body fluid, namely extracting blood of a patient taking the medicine in a sterile environment, and diluting the extracted blood by adopting double-distilled water with the volume of 1-2 times;
step two: and (3) red blood cell crushing treatment: placing the diluted blood into an ultrasonic power of 400-500W for acting for 25-30 min to carry out erythrocyte crushing treatment; after the red blood cells are crushed, filtering the crushed red blood cells through a filtering membrane to ensure that the crushed red blood cells are effectively separated;
step three: adding anhydrous ethanol and chloroform into the crushed erythrocyte liquid sequentially, stirring while adding, standing for 30min after stirring, putting the mixed liquid into a centrifuge for centrifugation, and collecting the centrifuged supernatant;
step four: shaking or swinging or rotating the supernatant collected in the third step, adding a nano gold or nano silver solution into the supernatant, uniformly mixing, and then carrying out centrifugal concentration;
step five: and C, sampling the liquid in the fourth step, carrying out transmission Raman spectrum detection on the obtained sample to obtain a drug molecule surface enhanced Raman spectrogram, and calculating through a model to obtain the drug content in the blood.
The device for detecting the concentration of a drug in a body fluid based on Raman spectroscopy, as shown in FIG. 1, comprises:
an ultrasonic cell disruption/dispersion machine 1 for disrupting red blood cells in the extracted blood by the ultrasonic cell disruption/dispersion machine 1;
a centrifugal separation device 2, wherein the liquid is injected into the centrifugal separation device 2 after being crushed by the ultrasonic cell crushing and dispersing machine 1, the centrifugal separation device 2 carries out centrifugal separation on the crushed red blood cell liquid, and supernatant is separated;
the mixed oscillation device 3 is used for feeding the supernatant separated by the centrifugal separation device 2 into the mixed oscillation device 3 to be mixed and oscillated with the nano-gold or nano-silver solution fed into the mixed oscillation device 3;
the centrifugal concentrator 4 is used for extracting the liquid mixed by the mixing and oscillating device 3 and adding the liquid into the centrifugal concentrator 4 for centrifugal concentration treatment;
and the Raman spectrometer 5 is used for sampling the liquid centrifugally concentrated by the centrifugal concentrator 4 and putting the liquid into the Raman spectrometer 5 for transmission Raman spectrum detection to obtain the concentration of the medicine in the blood.
In a specific using process, a liquid outlet of the ultrasonic cell disruption dispersion machine 1 can be communicated with the centrifugal separation device 2, a liquid outlet of the centrifugal separation device 2 is communicated with a liquid inlet of the mixing oscillation device 3, and a liquid outlet of the mixing oscillation device 3 is communicated with an inlet of the centrifugal concentrator 4; the ultrasonic cell disruption disperser 1, the centrifugal concentrator 4 and the Raman spectrometer 5 are conventional instruments, and are not described in detail herein;
specifically, when the device is used, the extracted blood is diluted and then added into an ultrasonic cell disruption dispersion machine 1, the diluted blood is subjected to erythrocyte disruption treatment in the ultrasonic cell disruption dispersion machine 1, the medicine in the erythrocytes is released after the erythrocyte disruption treatment, then the liquid after the disruption treatment is introduced into a mixing oscillation device 3 and then is fully mixed with the nanogold or nano-silver solution entering the mixing oscillation device, the nanogold or nano-silver solution absorbs the medicine in the liquid, the medicine is oscillated and mixed by the mixing oscillation device 3 in the mixing process, the mixed liquid enters a centrifugal concentrator 4 for centrifugal concentration, the liquid after the centrifugal concentration by the centrifugal concentrator is subjected to sample extraction, and then the sample extraction is put into a Raman spectrometer 5 for medicine concentration detection, the nano-gold or nano-silver liquid can increase the detection of Raman spectrum.
In the centrifugal separation process, in order to ensure the centrifugal separation efficiency, the centrifugal separation device 2 shown in fig. 2 includes a centrifugal outer cylinder 201 and a centrifugal inner cylinder 202 having a plurality of centrifugal holes on the circumferential direction, an outer cylinder cover 203 is fixedly connected to the upper end of the centrifugal outer cylinder 201, an inner cylinder cover 204 is fixedly connected to the upper end of the centrifugal inner cylinder 202, a liquid inlet pipe 205 is fixedly connected to the inner cylinder cover 204, the upper end of the liquid inlet pipe 205 passes through the outer cylinder cover 203 and extends outwards, and the outer wall of the liquid inlet pipe 205 is fixedly connected to the outer cylinder cover 203 through a bearing; the liquid inlet pipe 205 is provided with a one-way valve 206; the check valve can prevent liquid entering the centrifugal inner cylinder 202 from flowing back from the liquid inlet pipe, can protect the liquid entering the centrifugal inner cylinder and avoid being polluted by the external environment, and the liquid inlet pipe 205 is fixedly connected with the outer cylinder sealing cover through a bearing, so that the centrifugal inner cylinder can drive the liquid inlet pipe to rotate in the rotating process, and the sealing property of the outer cylinder sealing cover is not influenced;
an independent seal installation cavity 207 is formed in the bottom of the centrifugal outer cylinder 201, a centrifugal motor 208 is fixedly arranged in the independent seal installation cavity 207, a rotating shaft of the centrifugal motor 208 penetrates through the top of the independent seal installation cavity 207 and is fixedly connected with the bottom of the centrifugal inner cylinder 202, and the rotating shaft of the centrifugal motor 208 extending out of the independent seal installation cavity 207 is located in a seal rotating cylinder 209 movably connected with the bottom of the centrifugal inner cylinder 202 and the top of the independent seal installation cavity 207; the centrifugal motor can be ensured not to contact with centrifuged liquid by adopting an independent sealing cavity, so that pollution to the liquid is avoided, meanwhile, the bottom of the centrifugal inner cylinder and the top of the independent sealing installation cavity are movably connected through the sealing rotary cylinder, the centrifugal rotation of the centrifugal inner cylinder cannot be influenced while a rotating shaft of the centrifugal motor is separated from the centrifuged liquid, specifically, annular slots are arranged at the bottoms of the independent sealing installation cavity 207 and the centrifugal inner cylinder 202, the upper end and the lower end of the sealing rotary cylinder 209 are provided with outward-turned flanges as shown in figure 4, the flanges are placed into the annular slots, so that the isolation of the rotating shaft of the centrifugal motor is ensured, and the rotation of the centrifugal inner cylinder cannot be influenced;
a filtering membrane 210 is arranged between the outer side surface of the independent sealing installation cavity 207 and the inner side surface of the centrifugal outer cylinder 201, and a supernatant liquid outlet pipe 211 is communicated with the centrifugal outer cylinder 201 at the lower part of the filtering membrane 210;
specifically, in the using process, the crushed red blood cell liquid enters the centrifugal inner cylinder through the liquid inlet pipe, the centrifugal inner cylinder performs centrifugal motion under the action of the centrifugal motor at the moment, the red blood cell liquid is subjected to centrifugal separation, the separated liquid falls into the bottom of the centrifugal outer cylinder after being filtered by the filtering membrane 210 and then is kept stand, and after the liquid is kept stand, the liquid (supernatant) after the centrifugal separation is discharged through the supernatant liquid outlet pipe 211 and enters the mixing and vibrating device 3;
as shown in fig. 3, the hybrid oscillation device 3 includes a sound-insulation outer box 301 and an oscillation box 302 arranged in the sound-insulation outer box 301, two sides of the oscillation box 302 are fixedly connected with the inner wall of the sound-insulation box 301 through a plurality of horizontal springs 303, the bottom of the oscillation box 302 is fixedly connected with the bottom of the sound-insulation outer box 301 through a plurality of vertical springs 304, and an oscillation driving device for driving the oscillation box 301 to oscillate up and down in the sound-insulation outer box 301 is fixedly arranged at the bottom of the sound-insulation outer box 301; the sound insulation outer box body ensures the noise generated in the vibration process inside; the vibration box body can be connected with the sound insulation outer box body 301 through the arranged vertical spring and the horizontal spring, and meanwhile, the vibration box body can be guaranteed to vibrate in the sound insulation outer box body 301;
the vibration driving device comprises a driving motor 312 fixedly arranged in the sound insulation outer box body 301, a plurality of vertically downward push rods 309 fixedly connected to the vibration box body 302 and a driving shaft 314 fixedly connected with a rotating shaft of the driving motor 301, wherein two ends of the driving shaft 314 are movably connected with a shaft connecting seat 310 fixedly arranged at the bottom of the sound insulation outer box body 301, a plurality of cams 311 are arranged on the driving shaft 314, and the circumferential surface of each cams 311 is in contact with the lower end of the push rod 309;
the top of the oscillation box body 302 is communicated with a liquid adding hose 313, and the end part of the liquid adding hose 313 penetrates through the top of the sound insulation outer box body 301 and extends outwards; the bottom of the oscillating box body 302 is communicated with a mixed liquid outlet hose 308, and the end part of the mixed liquid outlet hose 308 penetrates through the side wall of the lower part of the sound insulation outer box body 301 and extends outwards; the upper parts of the outer sound insulation box body 301 and the oscillation box body 302 are respectively provided with an outer liquid inlet 305 and an inner liquid inlet 306, and the outer liquid inlet 305 and the inner liquid inlet 306 are communicated through a hose connecting assembly 307. The hose assembly comprises plugs which are hermetically plugged into the external liquid inlet 305 and the internal liquid inlet 306, and hoses which connect the plugs, the vibration of the vibration box body is not influenced by adopting the hose connection,
when using specifically, the supernatant after centrifugal separation enters into the oscillation box body through outer inlet 305, mix with nanogold or nano-silver solution that enters into the oscillation box body through adding liquid hose 313, driving motor 301 drives drive shaft 314 and rotates this moment, cam 311 that is equipped with on the drive shaft 314 is along with rotating, the cam promotes the ejector pin at the pivoted in-process and carries out the up-and-down motion, and then promote whole oscillation box 302 and carry out the vertical vibration, vibrate the processing to mixed liquid, liquid after the processing is discharged through mixed liquid play liquid hose 308, carry out subsequent processing.
The above embodiments are merely illustrative of the present invention, and should not be construed as limiting the scope of the present invention, and all designs identical or similar to the present invention are within the scope of the present invention.
Claims (10)
1. The method for measuring the body fluid drug concentration based on the Raman spectrum is characterized by comprising the following steps of:
, extracting body fluid, namely extracting blood of a patient taking the medicine in a sterile environment, and diluting the extracted blood;
step two: and (3) red blood cell crushing treatment: placing the diluted blood into an ultrasonic power of 400-500W for acting for 25-30 min to carry out erythrocyte crushing treatment;
step three: placing the crushed red blood cells into a centrifuge for centrifugal treatment, and then extracting supernatant of the red blood cells after the centrifugal treatment;
step four: shaking or swinging or rotating the supernatant collected in the third step, adding a nano gold or nano silver solution into the collected supernatant, uniformly mixing, and then carrying out centrifugal concentration;
step five: and C, sampling the liquid in the step four, then carrying out transmission Raman spectrum detection on the sampled liquid to obtain a drug molecule surface enhanced Raman spectrogram, and calculating through a model to obtain the drug content in the blood.
2. A method for measuring a drug concentration in a body fluid based on Raman spectroscopy according to claim 1, wherein in the second step, the broken red blood cells are filtered by a filter membrane after the red blood cells are broken.
3. A method for measuring the concentration of a drug in body fluid based on raman spectroscopy according to claim 1, wherein the extracted blood is diluted with PBS buffer in step .
4. The method for measuring the drug concentration in body fluid based on Raman spectroscopy of claim 1, wherein the extracted blood is diluted with 1-2 times volume of double distilled water in step .
5. The method for measuring the concentration of a drug in a body fluid based on Raman spectroscopy according to claim 4, wherein the third step is performed as follows: adding anhydrous ethanol and chloroform into the crushed erythrocyte liquid sequentially, stirring while adding, standing for 30min after stirring, putting the mixed liquid into a centrifuge for centrifugation, and collecting the centrifuged supernatant; and then the ethanol and the chloroform are removed by a nitrogen blowing instrument.
6. A method for measuring body fluid drug concentration based on Raman spectroscopy according to claim 3, wherein said PBS buffer is composed of the following materials in parts by weight:
8-12% of sodium dihydrogen phosphate, 5-10% of disodium hydrogen phosphate, 1-3% of potassium chloride, 0.5-1.5% of pancreatin and the balance of water.
7. The device for detecting the concentration of a drug in a body fluid based on Raman spectroscopy according to claim 1, comprising:
an ultrasonic cell disruption/dispersion machine (1) for disrupting red blood cells in the extracted blood by the ultrasonic cell disruption/dispersion machine (1);
the centrifugal separation device (2) is used for injecting liquid into the centrifugal separation device (2) after the liquid is crushed by the ultrasonic cell crushing and dispersing machine (1), and the centrifugal separation device (2) is used for carrying out centrifugal separation on the crushed red blood cell liquid and separating out supernatant;
the mixed oscillation device (3) is used for feeding the supernatant separated by the centrifugal separation device (2) into the mixed oscillation device (3) to be mixed and oscillated with the nano-gold or nano-silver solution fed into the mixed oscillation device (3);
the centrifugal concentrator (4) extracts the liquid mixed by the mixing and oscillating device (3) and adds the liquid into the centrifugal concentrator (4) for centrifugal concentration treatment;
and the Raman spectrometer (5) is used for sampling the liquid centrifugally concentrated by the centrifugal concentrator (4) and putting the liquid into the Raman spectrometer (5) for transmission Raman spectrum detection to obtain the concentration of the medicine in the blood.
8. The device for detecting the drug concentration in body fluid based on Raman spectrum according to claim 7, wherein the centrifugal separation device (2) comprises a centrifugal outer cylinder (201), a centrifugal inner cylinder (202) with a plurality of centrifugal holes arranged on the circumference, an outer cylinder cover (203) is fixedly connected to the upper end of the centrifugal outer cylinder (201), an inner cylinder cover (204) is fixedly connected to the upper end of the centrifugal inner cylinder (202), a liquid inlet pipe (205) is fixedly connected to the inner cylinder cover (204), the upper end of the liquid inlet pipe (205) extends outwards through the outer cylinder cover (203), and the outer wall of the liquid inlet pipe (205) is fixedly connected with the outer cylinder cover (203) through a bearing; a check valve (206) is arranged on the liquid inlet pipe (205);
an independent sealing installation cavity (207) is formed in the bottom of the centrifugal outer cylinder (201), a centrifugal motor (208) is fixedly arranged in the independent sealing installation cavity (207), a rotating shaft of the centrifugal motor (208) penetrates through the top of the independent sealing installation cavity (207) to be fixedly connected with the bottom of the centrifugal inner cylinder (202), and the rotating shaft of the centrifugal motor (208) extending out of the independent sealing installation cavity (207) is located in a sealing rotating cylinder (209) movably connected with the bottom of the centrifugal inner cylinder (202) and the top of the independent sealing installation cavity (207);
a filtering membrane (210) is arranged between the outer side surface of the independent sealing installation cavity (207) and the inner side surface of the centrifugal outer cylinder (201), and a supernatant liquid outlet pipe (211) is communicated with the centrifugal outer cylinder (201) at the lower part of the filtering membrane (210).
9. The device for detecting the concentration of a body fluid drug based on a Raman spectrum according to claim 7 or 8, wherein the mixing oscillation device (3) comprises an outer sound-insulation box (301) and an oscillation box (302) arranged in the outer sound-insulation box (301), two sides of the oscillation box (302) are fixedly connected with the inner wall of the outer sound-insulation box (301) through a plurality of horizontal springs (303), the bottom of the oscillation box (302) is fixedly connected with the bottom in the outer sound-insulation box (301) through a plurality of vertical springs (304), and an oscillation driving device for driving the oscillation box (301) to oscillate up and down in the outer sound-insulation box (301) is fixedly arranged at the bottom in the outer sound-insulation box (301);
the top of the oscillation box body (302) is communicated with a liquid adding hose (313), and the end part of the liquid adding hose (313) penetrates through the top of the sound insulation outer box body (301) to extend outwards; the bottom of the oscillating box body (302) is communicated with a mixed liquid outlet hose (308), and the end part of the mixed liquid outlet hose (308) penetrates through the side wall of the lower part of the sound insulation outer box body (301) to extend outwards; the upper parts of the outer sound insulation box body (301) and the vibration box body (302) are respectively provided with an outer liquid inlet (305) and an inner liquid inlet (306), and the outer liquid inlet (305) and the inner liquid inlet (306) are communicated through a hose connecting assembly (307).
10. The device for detecting the drug concentration in body fluid based on Raman spectrum according to claim 9, wherein the oscillation driving device comprises a driving motor (312) fixedly arranged in the outer sound-proof box (301), a plurality of vertically downward push rods (309) fixedly connected to the oscillation box (302), and a driving shaft (314) fixedly connected to a rotating shaft of the driving motor (301), two ends of the driving shaft (314) are movably connected to a shaft connecting seat (310) fixedly arranged at the bottom of the outer sound-proof box (301), the driving shaft (314) is provided with a plurality of cams (311), and the circumferential surface of each cams (311) is in contact with the lower end of the push rod (309).
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| CN115046984A (en) * | 2022-06-16 | 2022-09-13 | 海澳华(黑龙江)生物医药技术有限公司 | Method for detecting medicine in blood based on surface enhanced Raman spectroscopy technology |
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