CN106867890A - A kind of microdisk electrode Optimal Control System and method - Google Patents
A kind of microdisk electrode Optimal Control System and method Download PDFInfo
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Abstract
The present invention relates to a kind of microdisk electrode Optimal Control System, bioreactor includes casing and the LED light group set with casing outside；The casing is connected by gas flow controller, sensor unit and controller；LED light group is connected with controller；Place heating plate and cold plate in the bioreactor bottom.Its method includes：Setting time and CO2The relation value of flow and LED illumination intensity；Flow controller is according to set value calculation to the CO in bioreactor water tank2, air mass flow, LED controller according to setting LED illumination intensity level control LED brightness.The automaticity that the present invention solves existing photo-bioreactor system in China market is not very high, it is impossible to the actual demand of microalgae large-scale production is met well, so as to extensive this problem of industrialization culture of microalgae cannot be realized.The present invention greatly improves the automatization level of microdisk electrode process, shortens the microdisk electrode cycle, increases microalgae yield.
Description
Technical field
It is more particularly to a kind of to be used for microdisk electrode environmental factor Optimal Control System and method the invention belongs to experimental provision control technology field.
Background technology
Microalgae is most low, autotrophy aquatic unicellular microorganism.It is photosynthetic efficiency highest in nature, grows one of primitive organism species the most rapid, its biological substance for being rich in various high added values such as protein, amino acid, polysaccharide, vitamin, unrighted acid and pigment.
Microalgae can accumulate grease as biomass energy, and part algae kind such as splits kettle algae, and the fat content in dry cell weight can be converted into biodiesel up to 50%~77% after extraction.The nutritive salt that microalgae can also be efficiently gone in water removal, therefore microalgae can be further reduced as the three stage treatment unit of sewage treatment plant the nitrogen phosphorus level in secondary effluent, deep purifying sewage.
Microalgae industry culture in recent years is gradually risen, the particularly culture of some high added value microalgaes, such as salt algae, spirulina, rain give birth to red ball, but most domestic microdisk electrode is also in the chemurgy stage, live at the mercy of the elements it is outdoor open raise scattered, be unfavorable for the sustainable development of microdisk electrode.
The parameter of the detection of current heavy industrialization microdisk electrode bioreactor and control mainly has：Frond concentration, microbial metabolic products, for special algae kind or technique, will also be carried out on-line checking and real-time control by temperature, throughput, acid-base value, chlorophyll fluorescence etc. sometimes.
Microdisk electrode optical densitometric method is the effective way for obtaining micro algae growth situation, and nutrient solution is extracted from bioreactor by motor and flexible pipe, and measures its irradiation light and reflected light, and the optical density for calculating can quickly and accurately reflect the biomass of microalgae.
Micro algae growth relies primarily on photosynthesis, and illumination and carbon dioxide are all photosynthetic principal elements.Light application time length and intensity of illumination determine the photosynthetic efficiency of microalgae, and the growth rate to microalgae also has a significant impact；CO2Used as the substrate of photosynthesis of plant, its concentration is largely fixed micro algae growth situation, but is excessively passed through CO2Culture environment acidifying is also resulted in, microalgae mortality is caused；So carrying out CO in algae incubation2Amount, the optimal control of intensity of illumination are very necessary, are also current country's microdisk electrode industry key technology difficulty urgently to be resolved hurrily.
The content of the invention
It is an object of the present invention to provide a kind of microdisk electrode Optimal Control System and method, the influence for laboratory research environmental factor to micro algae growth, so as to instruct heavy industrialization microdisk electrode.
The technical scheme that is used to achieve the above object of the present invention is：A kind of microdisk electrode Optimal Control System, bioreactor includes casing and the LED light group set with casing outside；The casing is connected by gas flow controller, sensor unit and controller；LED light group is connected with controller；Place heating plate and cold plate in the bioreactor bottom.
The gas flow controller includes CO2Flow controller and air flow controller, are respectively used to conveying CO in casing2And air.
The sensor unit includes optical density detection means, temperature sensor and pH value sensor；Temperature sensor and pH value sensor are below the water surface of casing.
The optical density detection means includes laser, transparent vessel and luminous intensity probe；Transparent vessel and luminous intensity probe are set gradually in the Laser emission light path of the laser；The luminous intensity probe is connected with controller.
The transparent vessel is piston type column transparent vessel, and bottom is connected into below the water surface of water tank, for carrying out optical density detection to suction nutrient solution in container.
The heating plate and cold plate are placed in outside bioreactor bottom surface side by side.
A kind of microdisk electrode Optimal Control System, also including the small pump above casing, nutrition salt pump and alkali pump, is respectively used to be manually entered water, salt and alkali.
A kind of microdisk electrode optimal control method, comprises the following steps：
Setting time and CO2The relation value of flow and LED illumination intensity；
CO2CO of the flow controller according to setting2Flow value is controlled to the CO in bioreactor water tank2Flow；CO of the air flow controller according to setting2Flow value multiple is controlled to the air mass flow in bioreactor water tank；LED controller controls the brightness of LED according to the LED illumination intensity level of setting；
The microalgae when setting time is reached.
The setting time and CO2The relation value of flow and LED illumination intensity is comprised the following steps：
Time and optical density, CO are obtained by experiment2The relation curve of flow, LED illumination intensity；Setting boundary curve；Relation curve is different optical density, CO from the time corresponding to the intersection point of boundary curve2Flow, the corresponding setting time of LED illumination intensity.
The optical density gathered by optical density detection means forms the relation curve of actual optical density and time with the time, obtains the relation curve and time difference when setting optical density is reached with the ideal curve of setting；Setting time and time difference summation are obtained into actual collecting time for microalgae
The invention has the advantages that and advantage：
1. the characteristic such as the present invention has for microalgae culture system uncertainty, time-varying, delayed, by setting up in different CO2It is passed through under the conditions of concentration and intensity of illumination, the relational data model of optimal collecting time and optical density, so that the optimal collecting time under predicting current production status, greatly improves the production efficiency of bioreactor.
2. a kind of microdisk electrode Optimal Control System proposed by the invention, the automaticity for successfully solving existing photo-bioreactor system in China market is not very high, the actual demand of microalgae large-scale production can not well be met, so as to extensive this problem of industrialization culture of microalgae cannot be realized.
3rd, the present invention sets up dynamic, static data model by multigroup experiment, and the accurate prediction optimal collecting time of microdisk electrode greatly improves the automatization level of microdisk electrode process, shortens the microdisk electrode cycle, increases microalgae yield.
4th, the various automated control technologies of present invention application, can greatly improve accuracy, shortening cycle, the raising product yield of every control parameter, can also mitigate labor strength, improve operating efficiency, increase economic benefit.
Brief description of the drawings
Fig. 1 is Optimal Control System master-plan block diagram of the invention；
Fig. 2 is CO of the invention2Dynamic Data Model figure between concentration and intensity of illumination and optical density；
Fig. 3 is the static relation illustraton of model of optimal collecting time of the invention and optical density；
Fig. 4 is production application procedure chart of the invention；
Fig. 5 is the optimal collecting time schematic diagram of prediction of the invention.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is described in further detail.
Microdisk electrode Optimal Control System of the present invention master-plan launches around bioreactor.As shown in Figure 1, bioreactor size is 1.2m*0.3m, heating plate and cold plate are placed in left and right respectively immediately below its bottom, real time temperature is detected by PT100, control program is single closed loop PID, heating can not be opened simultaneously with refrigeration, and when error is less than 1 DEG C between Current Temperatures and design temperature, heating is simultaneously closed off with refrigeration.
Be arranged in parallel CO at bioreactor left lower 0.3m high2With air flow controller, for being passed through CO2With air gas mixture, CO necessary to be provided for microalgae photosynthesis2, its intake is drawn by controller, by flow controller execution.
Bioreactor tow sides hang with one group of LED light group, every group of 4 light bar, for intensity of illumination necessary to provide microalgae photosynthesis, by modbus agreements and its brightness of LED controller communications setting；LED illumination intensity is with its intensity of LED light group communications setting, so that LED light group curent change controls intensity of illumination by controller.Therefore, the corresponding relation of LED light group electric current and intensity of illumination is obtained by experiment, then LED light group electric current is controlled according to the LED illumination intensity level of setting by LED controller, and then control the brightness of LED.
Bioreactor top open part sets three pumps, according to pH value and liquid level state, carry out artificial recharge, build up one's health salt and mend alkali operation；Separately there are plug-in type pH detectors and optical density detector.
Wherein, optical density detector is made up of laser, transparent vessel (injector for medical purpose) and luminous intensity probe, rotated forward by controller controlled motor and pull syringe to measure solution suction transparent vessel in bioreactor, in transparent vessel side, laser is set, popped one's head in by the light intensity of opposite side and detect its transmission optical densities, solution is released back into bioreactor by motor reversal after the completion of detection.
By PLC collecting temperatures, pH, intensity of illumination, CO2With the data produced during the microdisk electrode such as air intake, optical density, delivered to via signal bus and can obtain optimal control policy in controller and be sent to each executing agency by control signal bus.Its method for optimally controlling step is：
1st, input CO is set up2It is passed through Dynamic Data Model between concentration and intensity of illumination and output optical density：
Same microalgae is directed to, a reasonable and different CO is set every time in laboratory environments2Concentration and intensity of illumination are passed through, and long lasting for its OD value of track record within the micro algae growth cycle.The experiment is repeated several times, CO can be input into2The Dynamic Data Model between concentration and intensity of illumination and output optical density is passed through, i.e.,
Wherein, CO2It is CO2Concentration, LED is fluorescent lamp group intensity of illumination, and OD is nutrient solution optical density.
As shown in Figure 2.Microalgae starts in the of short duration time of culture as a kind of simple aquatic slender cell space, and biomass does not substantially increase, microalgae number is doubled and redoubled after adapting to after a while, after reaching certain amount, due to factors such as environmental carrying capacities, biomass speedup substantially slows down so that constant.
2nd, the static relation model of optimal collecting time and optical density is set up：
As shown in figure 3, the different CO measured according to experiment2It is passed through the growth curve of microalgae under concentration and intensity of illumination, maximum is basically reached with biomass and speedup starts to slow down as principle, 5% is equal to as separation with biomass rate of rise, numerous separations can determine curve of demarcating, and the curve time corresponding with the intersection point of relation curve is optimal acquisition time.Different CO are obtained by the method for least square data fitting2The static relation model of optimal collecting time and optical density under the conditions of concentration, intensity of illumination, i.e.,
Wherein, CO2It is CO2Concentration, LED is fluorescent lamp group intensity of illumination, and OD is nutrient solution optical density.
3rd, the relational model of the optimal collecting time of gained and optical density will be tested as setting controller, as shown in figure 4, when new culture task is assigned, that is, when giving the biomass or collecting time of this culture, its corresponding CO can be determined according to setting controller2Concentration, intensity of illumination, wherein CO2Concentration set point is by adjusting CO2Realized with the ratio K of air, 485 communication modules and LED controller communications setting its brightness of the intensity of illumination by PLC.
4th, in large-scale industrial production, because optical density detector price is high, it is possible to a small amount of to be equipped with, shortage optical density sensor this case during microdisk electrode is directed to, CO is given by setting controller2The culture environments such as concentration, intensity of illumination, you can according to the optimal collecting time of static relation model prediction of optimal collecting time and optical density, as shown in Figure 5.
5th, optical density sampling Detection can be carried out for some bioreactors in incubation is industrialized, to tackle the situation that the actual incubation being likely to occur because external environment influences and ideal process are not inconsistent, predict that optimal collecting time is more accurate according to actual optical density change curve, while can also increase or correct CO2The Dynamic Data Model storehouse between concentration and intensity of illumination and optical density is passed through, predictablity rate is improved, microdisk electrode efficient stable is ensured.
Claims (10)
1. a kind of microdisk electrode Optimal Control System, it is characterised in that：Bioreactor includes casing and and case
The LED light group that external side is set；The casing passes through gas flow controller, sensor unit and controller
Connection；LED light group is connected with controller；Place heating plate and cold plate in the bioreactor bottom.
2. a kind of microdisk electrode Optimal Control System according to claim 1, it is characterised in that the gas stream
Amount controller includes CO2Flow controller and air flow controller, are respectively used to conveying CO in casing2With
Air.
3. a kind of microdisk electrode Optimal Control System according to claim 1, it is characterised in that the sensor
Unit includes optical density detection means, temperature sensor and pH value sensor；Temperature sensor and pH value are passed
Sensor is below the water surface of casing.
4. a kind of microdisk electrode Optimal Control System according to claim 3, it is characterised in that the optical density
Detection means includes laser, transparent vessel and luminous intensity probe；In the Laser emission light path of the laser
Set gradually transparent vessel and luminous intensity probe；The luminous intensity probe is connected with controller.
5. a kind of microdisk electrode Optimal Control System according to claim 4, it is characterised in that the transparent appearance
Device is piston type column transparent vessel, and bottom is connected into below the water surface of water tank, for suction culture in container
Liquid carries out optical density detection.
6. a kind of microdisk electrode Optimal Control System according to claim 1, it is characterised in that the heating plate
It is placed in side by side outside bioreactor bottom surface with cold plate.
7. a kind of microdisk electrode Optimal Control System according to claim 1, it is characterised in that also including being located at
Small pump, nutrition salt pump and alkali pump above casing, are respectively used to be manually entered water, salt and alkali.
8. a kind of microdisk electrode optimal control method, it is characterised in that comprise the following steps：
Setting time and CO2The relation value of flow and LED illumination intensity；
CO2CO of the flow controller according to setting2Flow value is controlled to the CO in bioreactor water tank2Stream
Amount；CO of the air flow controller according to setting2Flow value multiple is controlled to the sky in bioreactor water tank
Throughput；LED controller controls the brightness of LED according to the LED illumination intensity level of setting；
The microalgae when setting time is reached.
9. a kind of microdisk electrode optimal control method according to claim 8, it is characterised in that during the setting
Between and CO2The relation value of flow and LED illumination intensity is comprised the following steps：
Time and optical density, CO are obtained by experiment2The relation curve of flow, LED illumination intensity；Setting point
Boundary's curve；Relation curve is different optical density, CO from the time corresponding to the intersection point of boundary curve2Flow,
The corresponding setting time of LED illumination intensity.
10. a kind of microdisk electrode optimal control method according to claim 8, it is characterised in that：By light
The optical density of device for detecting density collection forms the relation curve of actual optical density and time with the time, is somebody's turn to do
Relation curve reaches time difference during setting optical density with the ideal curve of setting；By setting time and time
Difference summation obtains actual collecting time for microalgae.
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Cited By (3)
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| CN108004117A (en) * | 2017-12-01 | 2018-05-08 | 福建省东方利洋苗种繁育有限公司 | Micro algae culturing device |
| IT201900000355A1 (en) * | 2019-01-10 | 2020-07-10 | Thema Informatik Srl | Apparatus for the cultivation of algae in particular single-celled algae and a procedure for the cultivation of algae with an apparatus |
| CN113150975A (en) * | 2021-03-26 | 2021-07-23 | 中国科学院半导体研究所 | Photobioreactor and method for microalgae growth |
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| CN104713839A (en) * | 2013-12-13 | 2015-06-17 | 中国科学院大连化学物理研究所 | Automatic online microalgae OD determinator |
| CN105087371A (en) * | 2014-05-04 | 2015-11-25 | 北京工商大学 | Photobioreactor for automatically culturing microalgae |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004117A (en) * | 2017-12-01 | 2018-05-08 | 福建省东方利洋苗种繁育有限公司 | Micro algae culturing device |
| IT201900000355A1 (en) * | 2019-01-10 | 2020-07-10 | Thema Informatik Srl | Apparatus for the cultivation of algae in particular single-celled algae and a procedure for the cultivation of algae with an apparatus |
| CN113150975A (en) * | 2021-03-26 | 2021-07-23 | 中国科学院半导体研究所 | Photobioreactor and method for microalgae growth |
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Application publication date: 20170620 |