CN112063521A - Coliform group on-line measuring system - Google Patents
Coliform group on-line measuring system Download PDFInfo
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- CN112063521A CN112063521A CN202010973178.XA CN202010973178A CN112063521A CN 112063521 A CN112063521 A CN 112063521A CN 202010973178 A CN202010973178 A CN 202010973178A CN 112063521 A CN112063521 A CN 112063521A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/07—Means for pre-treatment of biological substances by electrical or electromagnetic forces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
Abstract
The invention discloses an online detection system for coliform groups.A reagent storage unit respectively stores cleaning fluid, culture solution and sample solution; an unused disposable sterile hole disc is stored in the hole disc liquid-filling packaging unit, the liquid mixing and transferring unit absorbs and transfers the liquid filled in the reagent storage unit to the unused disposable sterile hole disc, and the hole disc liquid-filling packaging unit seals the hole disc after the liquid is filled; the hole plate transfer unit is used for accommodating the sealed hole plate and transmitting the hole plate to the culture detection unit, the culture detection unit is used for carrying out constant-temperature culture on the hole plate and carrying out image recognition on the hole plate after a period of culture, and the control processing module is used for giving out a detection result according to the color change of each hole identified by the image and the number of holes with color change and the data in the maximum possible number table, so that the online detection of coliform groups is realized; the system can realize automatic online detection, manual operation is not needed, the hole plate is disposable, and the culture vessel is prevented from affecting a detection result due to unclean cleaning.
Description
Technical Field
The invention relates to the technical field of water quality detection, and further relates to an online detection system for coliform groups.
Background
Escherichia coli is a bacterium with the largest quantity in human and animal excreta, and is a universal index which is internationally recognized for checking the safety of various water qualities and food epidemiology. The more Escherichia coli, the higher the pollution degree of water source by human and animal feces, the higher the possibility of pathogenic bacteria exists, and the potential danger is brought to human health, especially the enterohemorrhagic Escherichia coli can cause human hemorrhagic enteritis and even death. In order to avoid the prevalence and outbreak of the related diseases, in recent years, some countries have performed HACCP (hazard analysis and critical control point) management, using detection of escherichia coli as a monitoring index of the microbial contamination condition and an evaluation index of the HACCP implementation effect.
The traditional detection method of the escherichia coli is a multi-tube fermentation Method (MTF) and a filter membrane filtration Method (MF), in order to achieve confirmation and quantitative detection, bacterial colonies are counted by a plate counting method after a sample is cultured in a culture dish for 24-72 hours, the culture operation is complex, the time consumption is long, the professional requirements are high, pathogenic bacteria with similar forms cannot be distinguished due to no specificity, the sensitivity is poor, and the rapid detection on site is difficult to realize. The enzyme-substrate method is used as a brand new detection method, the selective culture medium has the effects of inhibiting infectious microbes, the false positive is low, the quantification is accurate, the experimental operation process is convenient and quick, the detection result can be obtained in a short time, and the traditional detection method is replaced by a plurality of places at home and abroad to be used as a conventional detection method. At present, the design of the on-line monitoring instrument for escherichia coli at home and abroad refers to a laboratory enzyme-substrate method in the national standard, but the laboratory enzyme-substrate method depends on manual operation, and the automation is more and difficult to realize.
For those skilled in the art, how to automatically implement measurement of escherichia coli is a technical problem to be solved at present.
Disclosure of Invention
The invention provides an online detection system for coliform groups, which can automatically realize the measurement of the coliform groups and reduce the pollution in the culture process by utilizing a disposable hole disc, and the specific scheme is as follows:
an on-line coliform group detection system comprising:
the reagent storage unit is used for respectively containing cleaning fluid, culture solution and sample solution;
the liquid mixing and transferring unit can suck and transfer the liquid contained in the reagent storage unit;
the hole plate liquid filling and packaging unit is used for storing an unused disposable sterile hole plate, and the hole plate is used for containing the sample liquid transferred by the liquid mixing and transferring unit and sealing the hole plate;
the hole plate transferring unit is used for accommodating the hole plate supplied by the hole plate liquid-filling packaging unit and conveying the hole plate;
the culture detection unit is used for culturing the hole trays conveyed by the hole tray transfer unit at constant temperature, identifying images of the cultured hole trays, and controlling the processing module to give a detection result according to the color change of each hole identified by the images and the number of the holes with color change according to the data in the maximum possible number table.
Optionally, the liquid mixing and transferring unit includes a sampling head, a mechanical arm, a first sample suction pump, a sample mixing cup, a second sample suction pump, and an evacuation valve, the sampling head is driven by the mechanical arm to move, the first sample suction pump sucks the corresponding liquid in the reagent storage unit into the sample mixing cup, and a waste liquid in the sample mixing cup is discharged through the evacuation valve; the second sample suction pump is used for sucking the liquid in the sample mixing cup into the hole disc.
Optionally, the liquid mixing and transferring unit further comprises a closed bin, a magnetic stirrer and a first ultraviolet lamp, the sample mixing cup, the magnetic stirrer and the first ultraviolet lamp are located in the closed bin, and the magnetic stirrer is used for stirring the liquid in the sample mixing cup.
Optionally, the reagent storage unit includes a culture solution storage turntable, an aseptic water tank, a sodium hypochlorite solution tank, and a water sample tank, the culture solution storage turntable is used for driving the culture solution tube sealed and stored thereon to move, and the aseptic water contained in the aseptic water tank and the sodium hypochlorite solution contained in the sodium hypochlorite solution tank are used for cleaning the sample mixing cup.
Optionally, hole dish dress liquid encapsulation unit includes hole dish storage module and hole dish sealing module, hole dish storage module is used for storing the hole dish of piling up the placement, the bottom of hole dish storage module lateral wall sets up the passageway, and the hole dish of bottommost can be followed the lateral wall bottom of hole dish storage module is released, pushes away on the hole dish sealing module, the second inhales the appearance pump and follows the liquid that mixes the appearance cup absorption shifts to hole dish and seal on the hole dish sealing module, and the hole dish after sealing is followed the hole dish sealing module pushes away to hole dish transfer unit.
Optionally, the hole tray transfer unit includes a plurality of independently operating conveyor belts, and the hole tray sealing module pushes the hole tray to the middle of the hole tray transfer unit, so as to convey the hole tray to different culture detection units in different directions.
Optionally, the culture detection unit comprises a constant temperature module, and a fluorescent lamp, a second ultraviolet lamp and a camera are arranged in the constant temperature module; the constant temperature module is arranged at two ends of the hole disc transfer unit, and the hole disc transfer unit moves the hole disc into the constant temperature module.
Optionally, the tail end of the hole tray transfer unit is provided with a waste hole tray recovery box, and the hole tray after detection is driven by the hole tray transfer unit to be pushed to the waste hole tray recovery box.
The invention provides an online detection system for coliform groups, which is characterized in that a reagent storage unit is respectively used for containing cleaning fluid, culture solution and sample solution; the hole plate liquid filling and packaging unit is used for storing an unused disposable sterile hole plate, the liquid mixing and transferring unit absorbs and transfers the liquid filled in the reagent storage unit to the unused disposable sterile hole plate, and the hole plate liquid filling and packaging unit seals the hole plate after the liquid is filled; the hole plate transfer unit is used for accommodating the hole plate after sealing and packaging, and transmitting the hole plate to the culture detection unit, the culture detection unit is used for carrying out constant-temperature culture on the hole plate and carrying out image recognition on the hole plate after a period of culture, and the control processing module is used for giving out a detection result according to the color change of each hole identified by the image and the number of holes with color change according to the data in the maximum possible number table, so that the online detection of coliform groups is realized; the system can realize automatic online detection, does not need manual operation, has low requirement on the manual operation, and can prevent the culture vessel from affecting the detection result due to unclean cleaning due to the disposable use of the hole plate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic view of the overall structure of the online detecting system for coliform bacteria provided by the present invention.
The figure includes:
Detailed Description
The core of the invention is to provide an online detection system for coliform groups, which can automatically realize the measurement of the coliform groups and reduce the pollution in the culture process by utilizing a disposable hole disc.
In order to make those skilled in the art better understand the technical solution of the present invention, the online coliform group detection system of the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1, it is a schematic view of the overall structure of the online detecting system for coliform bacteria provided by the present invention; the coliform group online detection system comprises a reagent storage unit 1, a liquid mixing and transferring unit 2, a hole disc liquid filling and packaging unit 3, a hole disc transferring unit 4, a culture detection unit 5 and the like, wherein the reagent storage unit 1 is used for respectively containing cleaning liquid, culture liquid and sample liquid, and the cleaning liquid, the culture liquid and the sample liquid are contained in independent containers and are mutually isolated.
The liquid mixing and transferring unit 2 can suck and transfer the liquid contained in the reagent storage unit 1 to a well plate or the like.
The well plate liquid-containing packaging unit 3 is used for storing an unused disposable sterile well plate, and usually uses a 97-well quantitative plate on which 96 culture liquid tanks for culturing coliform bacteria and an overflow liquid tank for storing an overflow culture liquid are arranged. The hole plate is used for containing the sample liquid transferred by the liquid mixing and transferring unit 2, and after the sample liquid is added, the hole plate liquid containing packaging unit 3 packages the hole plate sealing opening, so that each groove on the hole plate forms an independent closed space; the hole plate is packaged on the upper surface of the hole plate and is pasted with a sealing film, the opening of the groove is sealed by the sealing film, the sealing film is made of transparent materials, and the sealing film does not need to be opened in the photographing process of the subsequent culture detection unit 5.
The hole plate transfer unit 4 receives the hole plate supplied by the hole plate liquid-containing packaging unit 3 and conveys the hole plate; when the well plate is sealed, the well plate is transferred to the well plate transfer unit 4, and the well plate filled with the culture sample is transferred to the culture detection unit 5 by the well plate transfer unit 4.
The culture detection unit 5 is used for culturing the hole plate conveyed by the hole plate transfer unit 4 at a constant temperature, culturing a culture sample in the hole plate at a proper temperature for a period of time, and carrying out image recognition on the cultured hole plate after the culture is finished. The maximum possible number is suitable for determining populations that are not dominant in a heterogeneous microbial community, but have specific physiological functions.
The coliform group online detection system can realize automatic online detection, does not need manual operation, has low requirement on manual operation, and prevents the culture vessel from influencing the detection result due to unclean cleaning because the hole plate is used once.
On the basis of the scheme, the liquid mixing and transferring unit 2 comprises a sampling head 21, a mechanical arm 22, a first sample suction pump 23, a sample mixing cup 24, a second sample suction pump 25 and an exhaust valve 26, wherein the sampling head 21 is driven by the mechanical arm 22 to move, and the sampling head 21 can move in the spatial dimensions of up, down, left, right, front and back so as to suck liquid at different positions conveniently. The sampling head 21 can stretch into the liquid below the liquid level to absorb the liquid, a pipeline is arranged between the sampling head 21 and the sample mixing cup 24, a first sample suction pump 23 is arranged on the pipeline, the corresponding liquid in the reagent storage unit 1 is sucked into the sample mixing cup 24 through the first sample suction pump 23, an emptying pipeline is arranged at the bottom of the sample mixing cup 24, an emptying valve 26 is arranged on the emptying pipeline, and the waste liquid in the sample mixing cup 24 is discharged through the emptying valve 26. The bottom of the sample mixing cup 24 is connected with a pipeline, a second sample suction pump 25 is arranged on the pipeline, and the second sample suction pump 25 is used for sucking the liquid in the sample mixing cup 24 into the hole plate.
Specifically, the liquid mixing and transferring unit 2 further comprises a closed bin 27, a magnetic stirrer 28 and a first ultraviolet lamp 29, the sample mixing cup 24, the magnetic stirrer 28 and the first ultraviolet lamp 29 are located in the closed bin 27, the sample mixing cup 24 is used for mixing samples in a closed environment, interference caused by microorganisms in air is avoided, and the inside of the closed bin 27 is sterilized and disinfected through the first ultraviolet lamp 29. The magnetic stirrer 28 is used for stirring the liquid in the sample mixing cup 24, the magnetic stirrer 28 comprises a magnetic induction coil positioned outside the sample mixing cup 24 and a rotor positioned inside the sample mixing cup 24, the rotor is immersed below the liquid level, the magnetic induction coil controls the rotor to move in the cup through the change of magnetic force to realize stirring, and the liquid in the sample mixing cup 24 is uniformly mixed.
The reagent storage unit 1 comprises a culture solution storage turntable 11, an aseptic water tank 12, a sodium hypochlorite solution tank 13 and a water sample tank 14, wherein a plurality of culture solution pipes are arranged on the culture solution storage turntable 11, the upper parts of the culture solution pipes are sealed, the culture solution storage turntable 11 is used for driving the culture solution pipes arranged on the culture solution storage turntable to move, the culture solution provides nutrients for bacterial reproduction, the culture solution pipes continuously move through the continuous rotation of the culture solution storage turntable 11, the culture solution pipes after being pumped out are taken down and are re-filled into new culture solution pipes, and a sampling head 21 downwards moves to puncture a cover film on the culture solution pipes when extracting the culture solution. Sterile water is filled in the sterile water tank 12, a sodium hypochlorite solution is filled in the sodium hypochlorite solution tank 13, and the sterile water and the sodium hypochlorite solution are used as cleaning liquids for cleaning the sample mixing cup 24. Before each operation, sodium hypochlorite solution is firstly added into the sample mixing cup 24 through the sampling head 21 for disinfection and sterilization, and then sterile water is added for washing, and the sample solution is transferred after the completion of the washing and the sterilization. The sterile water tank 12 is connected with a sterile water bucket through a water pipe, and sterile water is pumped into the sterile water tank 12 through a water pump; the sodium hypochlorite solution tank 13 is connected with a sodium hypochlorite barrel through a water pipe, and the sodium hypochlorite solution is pumped into the sodium hypochlorite solution tank 13 through a water pump, and sterile water and the sodium hypochlorite solution are pumped as required.
The water sample tank 14 is used for containing a sample solution, and the sample solution and the culture solution are respectively added into the sample mixing cup for mixing during operation, and are pumped into the hole plate after mixing.
The hole plate liquid filling and packaging unit 3 comprises a hole plate storage module 31 and a hole plate sealing module 32, wherein the hole plate storage module 31 is used for storing stacked hole plates and limiting the stacked hole plates; the bottom of the side wall of the hole tray storage module 31 is provided with a channel, the channel is arranged at the bottom of two opposite side walls, the height of the channel can accommodate one hole tray to move out, as shown in fig. 1, an external push rod pushes the lowest hole tray from right to left to push the lowest hole tray to the left, the second and above hole trays from bottom to top are blocked by the side wall of the hole tray storage module 31, the hole tray storage module 31 cannot be moved out, and only one hole tray is pushed out at a time.
The hole tray at the bottommost part is pushed to the hole tray sealing module 32 from the bottom of the side wall of the hole tray storage module 31, the sample mixed liquid sucked by the sample mixing cup 24 is transferred to the hole tray on the hole tray sealing module 32 by the second sample sucking pump 25, the hole tray is sealed by the hole tray sealing module 32 after the sample mixed liquid is completed, and the hole tray after the sample mixed liquid is sealed is pushed to the hole tray transfer unit 4 from the hole tray sealing module 32.
As shown in fig. 1, the hole plate transfer unit 4 includes a plurality of independently operating conveyor belts, each of which can independently control forward and reverse rotation to transfer the hole plates in different directions. The hole plate sealing module 32 is located in the middle of the hole plate transfer unit 4, the hole plate sealing module 32 pushes the hole plate to the middle of the hole plate transfer unit 4, the hole plate is conveyed to different culture detection units 5 in different directions through forward rotation or reverse rotation of a conveying belt, the hole plate stops moving after reaching the culture detection units 5, corresponding time is set for constant-temperature culture, and image recognition detection is carried out after the culture is finished. Two culture detection units 5 are arranged in the device shown in figure 1, and can be independently heated and cultured at the same time, different culture temperatures can be set, and multi-condition detection is realized.
Specifically, the culture detection unit 5 includes a constant temperature module 51, the constant temperature module 51 is a hollow box, and the constant temperature module maintains the temperature in the box to be constant through a temperature control device, and the temperature is typically 37 ℃ or 44.5 ℃.
A fluorescent lamp 52, a second ultraviolet lamp 53 and a camera 54 are arranged in the constant temperature module 51, and the fluorescent lamp 52 and the second ultraviolet lamp 53 are positioned on the upper surface of the inner cavity of the constant temperature module 51 and used for irradiating the hole disc; openings are formed in the bottoms of the two opposite side walls of the constant temperature module 51, the hole plate transfer unit 4 penetrates through the openings, the constant temperature module 51 is arranged at the two ends of the hole plate transfer unit 4, the hole plate is moved into the constant temperature module 51 by the hole plate transfer unit 4, and the hole plate is placed still and cultured at constant temperature after being moved.
Furthermore, set up abandonment hole dish collection box 6 at the end of hole dish transfer unit 4, the hole dish that detects the completion is driven by hole dish transfer unit 4 and is pushed to abandonment hole dish collection box 6, and hole dish transfer unit 4 continues to drive the hole dish and removes forward after detecting the completion, and the hole dish reaches and falls into abandonment hole dish collection box 6 after the end.
The coliform group on-line detection system comprises three detection modes of total coliform group detection, Escherichia coli detection and faecal coliform group detection, can be set according to requirements, and after the setting is successful, an instrument runs an appointed flow to complete the detection process.
Total coliform group detection mode
And (3) cleaning and sterilizing the sample mixing cup: the sampling head 21 extracts sodium hypochlorite solution from the sodium hypochlorite solution tank 13 to the sample mixing cup 24, the sample mixing cup 24 is sterilized and disinfected, and after the sterilization and disinfection, the emptying valve 26 is opened to discharge waste liquid out of the sample mixing cup 24; the sampling head 21 extracts the sterile water from the sterile water tank 12 to flush the mixing cup 24, and the emptying valve 26 is opened to discharge the waste liquid out of the mixing cup 24 after the completion. The first ultraviolet lamp 29 is turned on to sterilize the inside of the sealed cabin 27, and the first ultraviolet lamp 29 is turned off after a certain period of time.
Culture sample mixing and sample adding: taking out a hole plate from the hole plate storage module 31, starting the first sample suction pump 23, extracting a sample solution from the water sample tank 14 through the sampling head 21, extracting a culture solution from a culture solution pipe on the culture solution storage rotary plate 11, conveying the culture solution to the sample mixing cup 24, and starting the magnetic stirrer 28 to sufficiently and uniformly mix the sample mixed solution in the sample mixing cup 24. After the mixing is completed, the second sample suction pump 25 is turned on to pump the sample mixed solution into the well plate.
And (3) hole disc sealing and transferring: after the sample adding is completed, the hole plate sealing module 32 is started to cover and seal the hole plate, the hole plate is pushed to the hole plate transfer unit 4 from the hole plate sealing module 32, the hole plate transfer unit 4 conveys the hole plate to the inside of the constant temperature module 51 and then stops, and the temperature of the incubator is adjusted to 37 ℃ to start culture.
A detection result reading and analyzing link: after the incubation time is over, the fluorescent lamp 52 is turned on, the camera 54 recognizes the image of the hole plate and transmits the image to the control processing module, and the control processing module analyzes the color change condition of each hole of the hole plate and compares the color change condition with the data in the MPN table (maximum possible number table) to give a detection result.
And (3) waste disc recovery link: after the detection is finished, the hole plate transfer unit 4 is started to drive the hole plate to move to the end and then push the hole plate to the waste hole plate recovery box 6, and the waste hole plate recovery box is cleaned by operation and maintenance personnel during operation and maintenance.
The operation process of the Escherichia coli detection and the fecal coliform detection is similar to the total Escherichia coli detection mode, and the difference lies in the conditions of culture and detection, the culture heat preservation temperature of the Escherichia coli detection is 37 ℃, and the culture heat preservation temperature of the fecal coliform detection is 44.5 ℃; when the Escherichia coli is detected, the fluorescent lamp 52 is turned on, the camera 54 image-identifies the images of the hole disc, the fluorescent lamp 52 is turned off after the image identification is completed, the second ultraviolet lamp 53 is turned on for irradiation, the camera 54 image-identifies the images of the hole disc again, the two groups of images are transmitted to the control processing module, and the two groups of images are analyzed and compared according to the information of the two groups of images.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. An online detection system for coliform bacteria, comprising:
a reagent storage unit (1) for respectively storing a cleaning solution, a culture solution and a sample solution;
a liquid mixing and transferring unit (2) which can suck and transfer the liquid contained in the reagent storage unit (1);
the hole plate liquid filling and packaging unit (3) is used for storing an unused disposable sterile hole plate, and the hole plate is used for filling the sample liquid transferred by the liquid mixing and transferring unit (2) and sealing the hole plate;
a hole plate transfer unit (4) for receiving the hole plate supplied from the hole plate liquid-containing packaging unit (3) and transferring the hole plate;
and the culture detection unit (5) is used for culturing the hole trays conveyed by the hole tray transfer unit (4) at constant temperature, identifying images of the cultured hole trays, and controlling the processing module to give a detection result according to the color change of each hole identified by the images and the number of holes with color change according to the data in the maximum possible number table.
2. The coliform group online detection system of claim 1, wherein the liquid mixing and transferring unit (2) comprises a sampling head (21), a mechanical arm (22), a first sample sucking pump (23), a sample mixing cup (24), a second sample sucking pump (25) and an emptying valve (26), the sampling head (21) is driven by the mechanical arm (22) to move, the first sample sucking pump (23) sucks the corresponding liquid in the reagent storage unit (1) into the sample mixing cup (24), and the waste liquid in the sample mixing cup (24) is discharged through the emptying valve (26); the second sample suction pump (25) is used for sucking the liquid in the sample mixing cup (24) into the hole plate.
3. The coliform group online detection system of claim 2, wherein the liquid mixing and transferring unit (2) further comprises a closed bin (27), a magnetic stirrer (28) and a first ultraviolet lamp (29), the sample mixing cup (24), the magnetic stirrer (28) and the first ultraviolet lamp (29) are located in the closed bin (27), and the magnetic stirrer (28) is used for stirring the liquid in the sample mixing cup (24).
4. The coliform group online detection system according to claim 2, wherein the reagent storage unit (1) comprises a culture solution storage turntable (11), a sterile water tank (12), a sodium hypochlorite solution tank (13) and a water sample tank (14), the culture solution storage turntable (11) is used for driving the culture solution tube sealed and stored on the culture solution storage turntable to move, and the sterile water contained in the sterile water tank (12) and the sodium hypochlorite solution contained in the sodium hypochlorite solution tank (13) are used for cleaning the sample mixing cup (24).
5. The coliform group online detection system of claim 2, wherein the well plate liquid filling and packaging unit (3) comprises a well plate storage module (31) and a well plate sealing module (32), the well plate storage module (31) is used for storing stacked well plates, a channel is arranged at the bottom of the side wall of the well plate storage module (31), the bottommost well plate can be pushed out from the bottom of the side wall of the well plate storage module (31) and pushed onto the well plate sealing module (32), the second sample suction pump (25) transfers the liquid sucked by the sample mixing cup (24) to the well plate on the well plate sealing module (32) and seals the well plate, and the sealed well plate is pushed to the well plate transfer unit (4) from the well plate sealing module (32).
6. The coliform group online testing system of claim 5, wherein said tray transfer unit (4) comprises a plurality of independently operated conveyor belts, and said tray sealing module (32) pushes trays to the middle of said tray transfer unit (4) and can convey trays to different said culture testing units (5) in different directions.
7. The coliform group online detection system of claim 2, wherein the culture detection unit (5) comprises a constant temperature module (51), and a fluorescent lamp (52), a second ultraviolet lamp (53) and a camera (54) are arranged in the constant temperature module (51); the constant temperature modules (51) are arranged at two ends of the hole disc transfer unit (4), and the hole disc transfer unit (4) moves the hole disc into the constant temperature modules (51).
8. The coliform group online detection system according to claim 2, wherein a waste hole tray recovery box (6) is arranged at the end of the hole tray transfer unit (4), and the detected hole trays are pushed to the waste hole tray recovery box (6) by the hole tray transfer unit (4).
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