CN116139315B - Detection device for steam sterilizer - Google Patents
Detection device for steam sterilizer Download PDFInfo
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- CN116139315B CN116139315B CN202310306023.4A CN202310306023A CN116139315B CN 116139315 B CN116139315 B CN 116139315B CN 202310306023 A CN202310306023 A CN 202310306023A CN 116139315 B CN116139315 B CN 116139315B
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- detection device
- sterilizer
- rotary platform
- steam sterilizer
- water outlet
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
- A61L2/28—Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Analytical Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of sterilizers, in particular to a detection device for a steam sterilizer, which comprises a sterilizer, wherein an air outlet valve at the middle lower part of the sterilizer is connected with the detection device; the upper end of the condensing pipe is connected with an air outlet valve; 3 conveying belts are respectively arranged at the bottom of the detection device from left to right, conical flasks, a centrifugal machine and test tubes are respectively connected to the 3 conveying belts, an observation mirror is arranged on one side of the conveying belt on the right side, and a beaker is arranged on one side of the observation mirror; the middle part of the top of the detection device is provided with a rail, the rail is connected with a driving part, and the driving part is connected with a liquid transfer device through a telescopic rod; the rotary platform is installed on the right side of the detection device, the connecting frame is connected to the outer side of the rotary platform, a plurality of glass slides are placed on the rotary platform, the biological microscope is arranged on the rotary platform, and the biological microscope is connected with the top of the detection device through the fixing rod. The invention can detect the bacteria inactivation condition in the steam sterilizer and save the electric power according to the inactivation condition.
Description
Technical Field
The invention relates to the technical field of sterilizers, in particular to a detection device for a steam sterilizer.
Background
The sterilizer is an instrument capable of killing or removing all microorganisms on a transmission medium, including bacterial spores and nonpathogenic microorganisms, and comprises three modes of wet heat sterilization, dry heat sterilization and chemical sterilization; the common steam sterilizer is based on that steam formed during boiling of water cannot be diffused to the outside and is accumulated in a sealed container, and under the airtight condition, the steam pressure is increased and the temperature is correspondingly increased along with the boiling of water, so that the sterilization effect is achieved.
In the case of using a steam sterilizer, generally, an experimental apparatus and nutrients to be sterilized are put into the sterilizer for sterilization, so that a long time is required for heating and sterilizing materials in order to avoid incomplete sterilization of the sterilizer, and a large amount of electricity is required to be consumed, which is a waste of resources to a certain extent.
Therefore, a detection device of a steam sterilizer is needed to solve the above problem of wasting electric power due to sterilization.
Disclosure of Invention
The invention aims to solve the technical problem of providing a detection device for a steam sterilizer, which can detect the bacteria inactivation condition in the steam sterilizer and save electric power according to the inactivation condition.
In order to solve the technical problems, the invention adopts the following technical scheme: the detection device for the steam sterilizer comprises a sterilizer, wherein an air outlet valve at the middle lower part of the sterilizer is connected with the detection device;
a condensing pipe is arranged on the left side of the detection device, and the upper end of the condensing pipe is connected with an air outlet valve;
3 conveying belts are respectively arranged at the bottom of the detection device from left to right, conical flasks, centrifuges and test tubes are respectively connected to the 3 conveying belts, an observation mirror is arranged on one side of the conveying belt on the right side, and a beaker is arranged on one side of the observation mirror;
the middle part of the top of the detection device is provided with a rail, the rail is connected with a driving part, and the driving part is connected with a liquid transfer device through a telescopic rod;
the detecting device comprises a detecting device, and is characterized in that a rotating platform is arranged on the right side of the detecting device, a connecting frame is connected to the outer side of the rotating platform, a first water outlet and a second water outlet are formed in the connecting frame, a plurality of glass slides are placed on the rotating platform, a manipulator is arranged on one side of the rotating platform, a biological microscope is arranged on the rotating platform, and the biological microscope is connected with the top of the detecting device through a fixing rod.
As a further scheme of the invention, the condensing tube is obliquely arranged, and the inclination angle range is as follows: 30-60 degrees.
As a further scheme of the invention, the number of the conical flask, the centrifuge and the test tube is at least 3 respectively, and the conical flask, the centrifuge and the test tube are arranged at equal intervals.
As a further scheme of the invention, the water outlet of the condensing pipe is positioned right above the conical flask.
As a further aspect of the present invention, the pipette is located directly above the conical flask.
As a further aspect of the invention, the test tube is filled with a biuret reagent.
As a further scheme of the invention, the first water outlet is a Mailan reagent, and the second water outlet is distilled water.
As a further scheme of the invention, the beaker is filled with alcohol.
As the technical scheme is adopted, the invention has the advantages and positive effects that:
1. judging whether living bacteria exist in the steam sterilizer or not by staining with a methylene reagent and checking the image which is stained with the methylene and is in a hollow state on a glass slide by a biological microscope;
2. the sterilization degree of the steam sterilizer is judged by quantitatively analyzing the amount of complex after the bacteria are deactivated according to the principle that the protein encounters a biuret reagent to turn purple;
3. comprehensively analyzing the inactivation condition of the steam sterilizer by adopting a biological microscope to examine the principle that bacteria and proteins dyed by the methylene blue encounter a biuret reagent to turn purple;
4. the invention can effectively solve the power consumption problem of the steam sterilizer, avoid the steam sterilizer from working for too long time, and has convenient and efficient use and good practicability.
Drawings
Fig. 1 is a schematic structural view of a detection device for a steam sterilizer of the present invention.
Fig. 2 is a top view of the conveyor belt.
In the figure: 1 is a sterilizer, 2 is an air outlet valve, 3 is a detection device, 4 is a condensing tube, 5 is a conveyor belt, 6 is a conical flask, 7 is a centrifuge, 8 is a test tube, 9 is a track, 10 is a driving component, 11 is a telescopic rod, 12 is a liquid shifter, 13 is a rotary platform, 14 is a connecting frame, 15 is a first water outlet, 16 is a second water outlet, 17 is a glass slide, 18 is a manipulator, 19 is a sight glass, 20 is a beaker, 21 is a biological microscope, and 22 is a fixed rod.
Detailed Description
The invention will be described in further detail with reference to the drawings and the detailed description.
As shown in fig. 1-2, the detection device for the steam sterilizer comprises a sterilizer 1, wherein a gas outlet valve 2 at the middle lower part of the sterilizer 1 is connected with a detection device 3;
a condensing pipe 4 is arranged on the left side of the detection device 3, and the upper end of the condensing pipe 4 is connected with an air outlet valve 2;
as can be seen from the above description, the detecting device 3 can detect whether the bacteria in the steam exhausted from the air outlet valve 2 are active or not to determine the inactivation degree of the sterilizer 1;
3 conveying belts 5 are respectively arranged at the bottom of the detection device 3 from left to right, conical flasks 6, centrifuges 7 and test tubes 8 are respectively connected to the 3 conveying belts 5, an observation mirror 19 is arranged on one side of the conveying belt 5 on the right side, and a beaker 20 is arranged on one side of the observation mirror 19;
from the above description, the condensed bacterial liquid is transferred from the conical flask 6 to the centrifuge tube in the centrifuge 7 by the pipetter 12 for centrifugation, then the bacterial liquid at the bottom of the centrifuge tube is transferred to the test tube 8 by the pipetter 12, the observation mirror 19 is used for observing the color change in the test tube 8, and the beaker 20 is used for cleaning the pipetter 12 to avoid the bacterial in the pipetter 12 from influencing the next detection result;
the middle part of the top of the detection device 3 is provided with a track 9, the track 9 is connected with a driving part 10, and the driving part 10 is connected with a liquid shifter 12 through a telescopic rod 11;
as can be seen from the above description, the driving part 10 can drive the pipetter 12 to reciprocate on the track 9 through the telescopic rod 11, so that the pipetter 12 can suck the bacterial liquid from the conical flask 6 into a centrifuge tube in the centrifuge 7, and can suck the bacterial liquid from the centrifuge tube onto the glass slide 17 and into the test tube 8;
the detection device is characterized in that a rotary platform 13 is arranged on the right side of the detection device 3, a connecting frame 14 is connected to the outer side of the rotary platform 13, a first water outlet 15 and a second water outlet 16 are arranged on the connecting frame 14, a plurality of glass slides 17 are placed on the rotary platform 13, a manipulator 18 is arranged on one side of the rotary platform 13, a biological microscope 21 is arranged on the rotary platform 13, and the biological microscope 21 is connected with the top of the detection device 3 through a fixing rod 22.
As can be seen from the above description, the rotating platform 13 can drive the slide glass 17 to rotate, when the bacteria drop is on the slide glass 17, the cover glass is covered on the slide glass 17 by the manipulator 18, the rotating platform 13 operates, the slide glass 17 sequentially passes through the first water outlet 15, the second water outlet 16 and the biological microscope 21, and the biological microscope 21 can inspect the image of the slide glass dyed by the flange and in a hollow state to determine whether the living bacteria exist in the steam sterilizer.
As a further scheme of the invention, the condensing tube 4 is obliquely arranged, and the range of the inclination angle is as follows: 30-60 degrees.
As can be seen from the above description, the preferred inclination angle of the condensation pipe 4 is 45 degrees, and when the hot steam passes through the air outlet valve 2, the hot steam can bring the bacteria together, and then flows into the conical flask 6 after being condensed by the condensation pipe 4, and the condensation pipe 4 is arranged obliquely, so that the bacteria liquid can be more conveniently condensed and smoothly flows into the conical flask 6.
As a further proposal of the invention, the number of the conical flask 6, the centrifuge 7 and the test tube 8 is at least 3 respectively, and the conical flask, the centrifuge 7 and the test tube 8 are arranged at equal intervals.
As can be seen from the above description, the conveyor belt 5 can drive the conical flask 6, the centrifuge 7 and the test tube 8 to be conveyed to the middle when running; the multiple conical flasks 6, the centrifugal machine 7 and the test tube 8 can be used for detecting for multiple times, so that inaccuracy in single or few times of detection is avoided, and the subsequent experimental error is influenced.
As a further scheme of the invention, the water outlet of the condensing pipe 4 is positioned right above the conical flask 6.
From the above description, the bacterial liquid condensed by the condenser tube 4 can smoothly flow into the conical flask 6, so as to avoid the bacterial liquid flowing out of the conical flask 6 due to the inadaptation of the placement position of the conical flask 6.
As a further aspect of the present invention, the pipette 12 is located directly above the conical flask 6.
From the above description, the pipette 12 can aspirate condensed bacterial liquid from the conical flask 6.
As a further scheme of the invention, the test tube 8 is filled with the biuret reagent.
From the above description, the main component of the inactivated bacterial strain is protein, and the protein can undergo a color change reaction with the biuret reagent in the test tube 8, so that the amount of the bacterial strain can be determined according to the amount of the complex.
As a further aspect of the present invention, the first water outlet 15 is a merland reagent, and the second water outlet 16 is distilled water.
As can be seen from the above description, the first water outlet 15 drops the blue reagent on the glass slide 17, and the second water outlet 16 washes out the excess blue reagent on the glass slide 17, so as to avoid affecting the observation of the biological microscope 21.
As a further aspect of the present invention, the beaker 20 contains alcohol.
As can be seen from the above description, after the liquid in the centrifuge tube 7 is sucked by the liquid-transferring device 12, in order to avoid influencing the result of the next detection, the driving part 10 drives the liquid-transferring device 12 to move into the beaker 20 through the telescopic rod 11, and repeatedly sucking and extruding alcohol, so that the alcohol can denature and inactivate the living bacteria.
The detection method of the detection device for the steam sterilizer comprises the following steps: the method comprises the following specific steps:
step one, an air outlet valve 2 is opened, and after the mixed gas of steam and bacteria is condensed by a condenser tube 4, the bacteria flow into a conical flask 6;
step two, the driving part 10 drives the liquid transfer device 12 to move to the position right above the conical flask 6 on the track 9 through the telescopic rod 11, the telescopic rod 11 is extended, after the liquid transfer device 12 absorbs the bacterial liquid, the telescopic rod 11 is contracted, the driving part 10 drives the liquid transfer device 12 to move to the position right above the centrifugal machine 7, after the telescopic rod 11 is extended, the liquid transfer device 12 extrudes the bacterial liquid into the centrifugal tube, and the centrifugal process is carried out;
thirdly, after centrifugation, the liquid transfer device 12 absorbs bacterial liquid from the bottom of the centrifuge tube, the driving part 10 drives the liquid transfer device 12 to move onto the glass slide 17 and drip bacterial liquid, then move to the position right above the test tube 8 and drip 1 drop bacterial liquid, the mechanical arm 18 covers the glass slide on the glass slide 17, the rotating platform 13 operates, and the glass slide 17 sequentially passes through the first water outlet 15, the second water outlet 16 and the biological microscope 21;
step four, the driving part 10 drives the liquid dispenser 12 to move to the position right above the beaker 20, the telescopic rod 11 is extended, and the liquid dispenser 12 repeatedly sucks and extrudes alcohol;
step five, the steps one to four are circularly operated for a plurality of times, and the controller records the observation conditions of the observation mirror 19 and the biological microscope 21.
The invention utilizes blue color of living bacteria in a hollow state after being dyed by the methylene blue, and solid blue color of dead bacteria, so as to distinguish the living bacteria from the dead bacteria; judging the degree of viable bacteria becoming dead bacteria by utilizing the principle that protein encounters a biuret reagent and turning purple, and when bacterial liquid at the bottom of a quantitative centrifuge tube is added into a test tube 8 for a plurality of times, keeping the total amount of the complex unchanged all the time, namely indicating that the total number of dead bacteria tends to be equal; the sterilization degree of the steam sterilizer can be obtained through comprehensive analysis of the steam sterilizer, the steam sterilizer is prevented from working for too long time, the electric quantity is saved, the use is convenient and efficient, and the practicability is good.
While particular embodiments of the present invention have been described above, it will be appreciated by those skilled in the art that these are merely illustrative, and that many variations or modifications may be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined only by the appended claims.
Claims (5)
1. The detection device for the steam sterilizer comprises a sterilizer (1) and is characterized in that: the air outlet valve (2) at the middle lower part of the sterilizer (1) is connected with a detection device (3);
a condensing pipe (4) is arranged on the left side of the detection device (3), and the upper end of the condensing pipe (4) is connected with an air outlet valve (2);
3 conveying belts (5) are respectively arranged at the bottom of the detection device (3) from left to right, conical flasks (6), centrifuges (7) and test tubes (8) are respectively connected to the 3 conveying belts (5), an observation mirror (19) is arranged on one side of the conveying belt (5) on the right side, and a beaker (20) is arranged on one side of the observation mirror (19);
the middle part of the top of the detection device (3) is provided with a track (9), the track (9) is connected with a driving part (10), and the driving part (10) is connected with a liquid transfer device (12) through a telescopic rod (11);
the right side of the detection device (3) is provided with a rotary platform (13), the outer side of the rotary platform (13) is connected with a connecting frame (14), the connecting frame (14) is provided with a first water outlet (15) and a second water outlet (16), the rotary platform (13) is provided with a plurality of glass slides (17), one side of the rotary platform (13) is provided with a manipulator (18), the rotary platform (13) is provided with a biological microscope (21), and the biological microscope (21) is connected with the top of the detection device (3) through a fixed rod (22);
the test tube (8) is filled with a biuret reagent; the first water outlet (15) is a methylene blue reagent, and the second water outlet (16) is distilled water; the beaker (20) is filled with alcohol.
2. The detection device for steam sterilizer as claimed in claim 1, wherein: the condenser pipe (4) is obliquely arranged, and the range of the inclination angle is as follows: 30-60 degrees.
3. The detection device for steam sterilizer as claimed in claim 1, wherein: the number of the conical flask (6), the centrifuge (7) and the test tube (8) is at least 3, and the conical flask, the centrifuge (7) and the test tube (8) are arranged at equal intervals.
4. The detection device for steam sterilizer as claimed in claim 1, wherein: the water outlet of the condensing pipe (4) is positioned right above the conical flask (6).
5. The detection device for steam sterilizer as claimed in claim 1, wherein: the pipettor (12) is positioned right above the conical flask (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310306023.4A CN116139315B (en) | 2023-03-27 | 2023-03-27 | Detection device for steam sterilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310306023.4A CN116139315B (en) | 2023-03-27 | 2023-03-27 | Detection device for steam sterilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116139315A CN116139315A (en) | 2023-05-23 |
| CN116139315B true CN116139315B (en) | 2023-10-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310306023.4A Active CN116139315B (en) | 2023-03-27 | 2023-03-27 | Detection device for steam sterilizer |
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| Country | Link |
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| CN (1) | CN116139315B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4145186A (en) * | 1976-10-04 | 1979-03-20 | H. W. Andersen Products Inc. | Sterilization detection device and method |
| JP2002224198A (en) * | 2001-02-05 | 2002-08-13 | Chiyoda Manufacturing Co Ltd | Steam sterilizer |
| CN101413872A (en) * | 2008-10-27 | 2009-04-22 | 吉林大学 | Method for rapidly detecting microorganism viable bacteria number |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN104056292A (en) * | 2014-07-15 | 2014-09-24 | 山东新华医疗器械股份有限公司 | Detection system for sterilizing effect of sterilizer |
| CN106075516A (en) * | 2016-07-26 | 2016-11-09 | 张剑 | A kind of pressuresteam sterilization process verification device and sterilizing effect monitoring method thereof |
| CN215953529U (en) * | 2021-08-01 | 2022-03-04 | 杭州氢源素生物科技有限公司 | Intelligent cleaning, disinfecting and sterilizing monitoring platform based on Internet of things technology |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2369830A1 (en) * | 1999-05-03 | 2000-11-09 | Ira C. Felkner | Methods, compositions and kits for biological indicator of sterilization |
-
2023
- 2023-03-27 CN CN202310306023.4A patent/CN116139315B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4145186A (en) * | 1976-10-04 | 1979-03-20 | H. W. Andersen Products Inc. | Sterilization detection device and method |
| JP2002224198A (en) * | 2001-02-05 | 2002-08-13 | Chiyoda Manufacturing Co Ltd | Steam sterilizer |
| CN101413872A (en) * | 2008-10-27 | 2009-04-22 | 吉林大学 | Method for rapidly detecting microorganism viable bacteria number |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN104056292A (en) * | 2014-07-15 | 2014-09-24 | 山东新华医疗器械股份有限公司 | Detection system for sterilizing effect of sterilizer |
| CN106075516A (en) * | 2016-07-26 | 2016-11-09 | 张剑 | A kind of pressuresteam sterilization process verification device and sterilizing effect monitoring method thereof |
| CN215953529U (en) * | 2021-08-01 | 2022-03-04 | 杭州氢源素生物科技有限公司 | Intelligent cleaning, disinfecting and sterilizing monitoring platform based on Internet of things technology |
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| CN116139315A (en) | 2023-05-23 |
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