CN113176417B

CN113176417B - Method for detecting blood conventional parameters and C-reactive protein parameters in blood sample

Info

Publication number: CN113176417B
Application number: CN202110296868.0A
Authority: CN
Inventors: 郁琦; 谢子贤; 李朝阳; 代勇; 易秋实; 叶燚
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2014-07-01
Filing date: 2014-07-01
Publication date: 2024-04-02
Anticipated expiration: 2034-07-01
Also published as: CN107656085A; CN107656068B; WO2016000216A1; CN107656085B; CN107656068A; CN105378478A; CN105378478B; CN113176417A

Abstract

The blood detector comprises a blood routine measuring module (1) and a C-reactive protein measuring module (2), wherein the C-reactive protein measuring module (2) comprises a reaction container, one or more measuring containers and a hemolytic agent conveying pipeline, the reaction container is used for receiving samples and latex reagents distributed by the sample collecting and distributing module, the measuring containers are used for providing measuring places for reaction liquid, the reaction container and the measuring containers are controllably communicated through the sample conveying pipeline, the control and information processing module also controls the liquid path supporting module to provide power, the hemolytic agent is added into the reaction container through the hemolytic agent conveying pipeline, the hemolytic agent reacts with blood samples and latex reagents which are added later in the reaction container, and then the samples are conveyed into the measuring containers through the sample conveying pipeline, so that the measuring efficiency of C-reactive protein parameters is improved, and the measurement of C-reactive protein parameters of a plurality of whole blood samples can be rapidly completed.

Description

Method for detecting blood conventional parameters and C-reactive protein parameters in blood sample

The application is a divisional application, the application number of the original application is 201480039632.7, the application date is 2014, 7 months and 1 day, and the invention is named as a whole blood sample detection method and a blood detector; and the application number is 201710877017.9, the delivery date is 2017-09-25, and the invention name is a blood detector.

Technical Field

The application relates to the field of blood detection and analysis, in particular to a blood detector which is used for supporting blood cell routine detection and CRP detection by using a whole blood sample alone.

Background

At present, it is often required in clinical diagnosis in hospitals to obtain the results of detection of both blood routine parameters and CRP (C-reactive protein) parameters of the patient's blood.

In most of the existing blood detection apparatuses, blood routine detection such as blood cell count and classification and CRP detection are performed on different apparatuses by using different types of samples, blood cell count and classification are generally performed on blood cell analysis by using whole blood samples, and CRP is measured on biochemical analysis or a special protein analyzer by using serum samples. Since blood routine and CRP are often used in combination clinically, hospitals currently require two samples to be taken from patients or an increased amount of blood to be collected, each on a different machine. Thus causing great pain to the patient, requiring detection on two machines and troublesome inspection operation.

In order to solve the above problems, it is necessary to develop an instrument for detecting blood routine parameters and CRP parameters using the same whole blood sample on one machine. Because of the different measurement methods of these parameters, multiple measurement channels are required to support the measurement of different parameters.

At present, a single-machine product is supported, and a whole blood sample can be used for blood routine parameter measurement and CRP parameter measurement, but the testing speed is slower, the highest testing speed is only 20 samples/hour, and the requirement on efficiency in clinical examination cannot be met.

Disclosure of Invention

The application provides a blood detector which can rapidly complete the measurement of blood routine parameters and CRP parameters on the same machine by adopting the same sample.

According to a first aspect of the present application, there is provided a blood test apparatus comprising:

a blood routine measurement module for providing a measurement location for the allocated sample, performing measurement on the allocated sample for the purpose of obtaining at least one blood routine parameter, and outputting a measurement result;

the C-reactive protein measuring module is used for providing a measuring place for the distributed sample, measuring the distributed sample for the purpose of obtaining C-reactive protein parameters and outputting a measuring result, and comprises a reaction container, at least one measuring container, a sample conveying pipeline and a hemolysis agent conveying pipeline, wherein the hemolysis agent conveying pipeline is used for adding a hemolysis agent into the reaction container, the reaction container also receives the whole blood sample and the latex reagent distributed by the sample collecting and distributing module, the reaction container and the measuring container are controllably communicated through the sample conveying pipeline, and the measuring container is used for providing a measuring place for a reaction liquid;

The sample collection and distribution module is used for collecting a whole blood sample and distributing the collected whole blood sample to the reaction containers in the blood routine measurement module and the C-reactive protein measurement module;

the liquid path support module is used for providing liquid path support for the sample acquisition and distribution module and each measurement module;

the control and information processing module is respectively coupled to the sample collecting and distributing module, each measuring module and the liquid path supporting module and is used for controlling the sample collecting and distributing module to collect whole blood samples and distribute the whole blood samples, controlling the liquid path supporting module to carry out fluid transportation, receiving measuring results output by each measuring module and processing the measuring results; when the sample is continuously measured, the control and information processing module controls the sample collecting and distributing module to collect the whole blood sample and distributes the collected whole blood sample to the reaction containers in the blood routine measuring module and the C-reactive protein measuring module, the control and information processing module also controls the liquid path supporting module to provide power, a hemolytic agent is added into the reaction containers through the hemolytic agent conveying pipeline, the hemolytic agent reacts with the whole blood sample and the latex reagent which are added later in the reaction containers, then the sample output by the reaction containers is conveyed into the measuring containers through the sample conveying pipeline, and when the blood routine measuring module finishes the blood routine measurement of the current whole blood sample and the current whole blood sample does not finish the measurement of the C-reactive protein parameter, the control and information processing module controls the sample collecting and distributing module to start the collection and distribution of the next whole blood sample and distributes the collected next whole blood sample to the reaction containers in the blood routine measuring module and the C-reactive protein measuring module.

According to a second aspect of the present application, there is provided another blood test apparatus comprising:

a blood routine measurement module for providing a measurement location for the dispensed sample, performing a measurement on the dispensed whole blood sample with the aim of obtaining at least one blood routine parameter and outputting a measurement result;

a C-reactive protein measurement module for providing a measurement site for the dispensed sample, performing measurement for the dispensed whole blood sample for the purpose of obtaining C-reactive protein parameters and outputting a measurement result, the C-reactive protein measurement module comprising a reaction vessel, a measurement vessel, a sample transport line, a CRP measurement cell waste liquid discharge mechanism, a reaction cell waste liquid discharge mechanism, and a hemolysis agent transport line for adding a hemolysis agent into the reaction vessel, the reaction vessel further receiving the whole blood sample and the latex reagent dispensed by the sample collection and dispensing module, the reaction vessel and the measurement vessel being controllably communicated through the sample transport line, the measurement vessel for providing a measurement site for the reaction liquid; the CRP measuring cell waste liquid discharging mechanism is communicated with the measuring container and used for discharging waste liquid in the measuring container, and the reaction cell waste liquid discharging mechanism is communicated with the reaction container and used for discharging waste liquid in the reaction container;

the control and information processing module is respectively coupled to the sample collecting and distributing module and each measuring module and is used for controlling the sample collecting and distributing module to collect whole blood samples and distribute the whole blood samples, receiving measuring results output by each measuring module and processing the measuring results; when the sample is continuously measured, the control and information processing module controls the sample collecting and distributing module to collect the whole blood sample, distributes the collected whole blood sample to the reaction containers in the blood routine measuring module and the C-reactive protein measuring module, controls the liquid path supporting module to provide power, adds a hemolytic agent into the reaction container through the hemolytic agent conveying pipeline, reacts with the whole blood sample and the latex reagent which are added later in the reaction container, and conveys the sample into the measuring container through the sample conveying pipeline.

In one embodiment, the control and information processing module controls the sample collection and distribution module to begin collection and distribution of a next whole blood sample when the blood routine measurement module ends the blood routine measurement of the current sample and the current sample does not complete the measurement of the C-reactive protein parameter, and distributes the collected next whole blood sample to the reaction vessels in the blood routine measurement module and the C-reactive protein measurement module.

In one embodiment, after the reaction vessel is operated, the control and information processing module controls the liquid path supporting module to drive the waste liquid in the reaction vessel to be discharged by the waste liquid discharge mechanism of the reaction tank; and/or after the measuring container finishes the operation, the control and information processing module controls the liquid path supporting module to drive the waste liquid in the measuring container to be discharged by the waste liquid discharging mechanism of the measuring CRP measuring pool.

In a further embodiment, after the reaction container finishes the operation and the measurement container does not finish the measurement of the C-reactive protein parameter of the current sample, the control and information processing module, namely the control liquid path support module, drives the waste liquid in the reaction container to be discharged by the reaction tank waste liquid discharging mechanism; and/or after the measuring container finishes the operation and the reaction container does not finish the reaction of the next whole blood sample, the control and information processing module controls the liquid path supporting module to drive the waste liquid in the measuring container to be discharged by the waste liquid discharging mechanism of the measuring CRP measuring pool.

Drawings

FIG. 1 is a sectional view of a blood test apparatus disclosed in example 1 of the present application;

FIG. 2 is a schematic block diagram of a blood detector disclosed in embodiment 1 of the present application;

FIG. 3 is a schematic block diagram showing another construction of the reactive protein measuring module of example 1C of the present application;

FIG. 4 is a schematic diagram of a sample continuous measurement strategy according to example 1 of the present application;

FIG. 5 is a schematic view showing the structure of a sample collection and distribution module according to embodiment 1 of the present application;

FIGS. 6a and 6b are schematic diagrams illustrating the segmented distribution of samples by the sample acquisition and distribution module of example 1 of the present application;

wherein,

FIG. 6a is a schematic illustration of the sample size of a single use collection in example 1 of the present application;

FIG. 6b is a schematic illustration of sample size after one dispensing in example 1 of the present application;

FIG. 7 is a schematic top view of an auto-sampling module according to embodiment 2 of the present application;

FIG. 8 is a schematic and schematic illustration of the structure of a latex reagent storage module in accordance with example 3 of the present application;

FIG. 9 is a flow chart of a method of testing whole blood samples in example 3 of the present application.

Detailed Description

In the embodiment of the application, the CRP parameter measurement function and the blood routine measurement function are integrated on the same blood detector, and the CRP measurement and the blood routine measurement are both obtained by adopting a whole blood sample, and the CRP measurement is obtained by firstly mixing the whole blood sample with the hemolytic agent and then adding the latex reagent. With this measurement mode, the measurement time of the CRP parameter is longer than that of the blood conventional parameter, for example, for the same sample, the blood conventional parameter measurement takes 1 minute, whereas the CRP parameter measurement takes 2 minutes, and if the CRP parameter measurement is waited after the completion of the blood conventional parameter measurement, the measurement speed of the blood conventional parameter is inevitably lowered. In order to rapidly complete measurement of blood routine parameters and CRP parameters on the same machine, in the embodiment of the present application, the C-reactive protein measurement module includes a plurality of measurement channels, and in the continuous measurement process of the sample, the plurality of measurement channels are added to the sample in turn according to a preset sequence and perform measurement. The present application will be described in detail with reference to the accompanying drawings.

Example 1:

please refer to fig. 1 and 2, which illustrate a structure of the blood detector disclosed in the present embodiment. Wherein, FIG. 1 is a schematic perspective view of a blood detector, and FIG. 2 is a schematic block diagram of the blood detector; the stippled arrow lines in fig. 2 are the electrical signal trend, and the solid arrow lines are the fluid path trend. The blood detector includes: a blood routine measurement module 1 (not shown in fig. 1), a C-reactive protein measurement module (hereinafter also referred to as CRP measurement module) 2, a sample collection and distribution module 3, a liquid path support module 8 (not shown in fig. 1), and a control and information processing module 9 (not shown in fig. 1). Wherein:

the blood routine measuring module 1 is used for providing a measuring place for the distributed sample, measuring the distributed sample for the purpose of obtaining at least one blood routine parameter and outputting a measuring result. Referring to fig. 1, in one embodiment, the blood routine measurement module 1 may be further subdivided into various sub-measurement modules according to measurement needs: WBC classification measurement module 11, WBC/HGB measurement module 12, and RBC/PLT measurement module 13. The WBC classification measurement module 11 is used for providing a place for completing the reaction for the allocated sample and measuring five classification results for obtaining WBCs; the WBC/HGB measurement module 12 is configured to perform WBC (white blood cell) count and morphological parameter measurement, and has a function of measuring HGB (hemoglobin); the RBC/PLT measurement module 13 is used to perform RBC (red blood cell), PLT (platelet) count and morphological parameter measurements. It should be noted that, each of the above sub-modules (11, 12 and 13) may be implemented by using an existing measurement mode, and other blood routine measurement sub-modules may be added or some of the above sub-modules may be reduced in the actual blood routine measurement process.

The C-reactive protein measuring module 2 is used for providing a measuring place for the distributed sample, measuring the distributed sample for the purpose of obtaining the C-reactive protein parameter and outputting a measuring result. After the sample is distributed to the C-reactive protein measuring module 2, the sample is reacted with the added hemolytic agent, then the latex reagent is added into the reaction liquid, finally the light transmission amount or the light scattering amount of the reaction liquid added with the latex reagent is detected by photoelectric detection, and the measuring result is output. In this application, the facility of providing a process from reaction, measurement to output of measurement results for a sample is collectively referred to as a measurement channel, which generally includes: a reaction vessel which can be realized as a reaction site for a sample and a reagent, a measurement vessel which can be realized as a reaction solution providing a measurement site, and a detection device which can be realized to measure the reaction solution in the measurement vessel and output a measurement result. In a specific implementation, the reaction vessel and the measurement vessel may be combined, and the reaction vessel may be used as a reaction site for a sample and a reagent, or may be used as a measurement site for a reaction solution.

In this embodiment of the present application, the C-reactive protein measurement module includes at least two measurement containers and at least one set of detection device, so as to implement a plurality of measurement channels, where each measurement channel includes a measurement container, the measurement container is used to provide a measurement location for the allocated sample, and the detection device performs measurement on the samples in the measurement containers respectively for obtaining the C-reactive protein parameter and outputs a measurement result. Because the reagent such as the whole blood sample, the hemolytic agent, the latex reagent and the like needs to be mixed and reacted for a preset time before the measurement of the C-reactive protein. Thus, in certain embodiments, the C-reactive protein measurement module comprises at least one reaction vessel, at least two measurement vessels, and at least one set of detection means, wherein the reaction vessel is in communication with the measurement vessels for providing a reaction site for the dispensed sample and reagent, and the dispensed sample and reagent are dispensed into the measurement vessels in a predetermined sequence after the reaction is completed for measurement of the C-reactive protein. In some embodiments, the reaction vessels and the detection devices are in one-to-one correspondence with the measurement vessels, i.e., each measurement channel comprises a reaction vessel, a detection device, and a measurement vessel. In further embodiments, the reaction vessels and/or detection devices are not in one-to-one correspondence with the measurement vessels, e.g. the number of reaction vessels and/or detection devices is less than the number of measurement vessels, the reaction vessels and/or detection devices being shared by a plurality of measurement channels. In this case, one measuring channel comprises a measuring vessel, a reaction vessel and/or a detection device which are common to the other measuring channels. In other embodiments, the C-reactive protein measurement module may also be devoid of a reaction vessel, the measurement vessel providing both a reaction site and a measurement site.

The sample collection and distribution module 3 is used for collecting a whole blood sample and distributing the collected sample to the blood routine measurement module 1 and the C-reactive protein measurement module 2. When the reaction vessel is included in the measurement channel, the sample collection and distribution module 3 distributes the collected sample to the reaction vessel; when no reaction vessel is included in the measurement channel, the sample collection and distribution module 3 distributes the collected sample to the measurement vessel.

The fluid path support module 8 provides fluid path support for the sample collection and distribution module and each measurement module. In a particular embodiment, the fluid path support module 8 generally includes: valves, pumps, and/or syringes, etc., are mainly used for transporting samples, reagents, and discharging waste liquid in blood test apparatuses.

The control and information processing module 9 is respectively coupled to the sample collection and distribution module 3, each measuring module and the liquid path supporting module 8, and is used for controlling the sample collection and distribution module 3 to collect samples and distribute the samples, controlling the liquid path supporting module 8 to carry out fluid transportation, receiving the measuring results output by each measuring module and processing the measuring results. In this embodiment, the control and information processing module controls the sample collection and distribution module to distribute the samples collected each time to one measurement channel of the blood routine measurement module and the C-reactive protein measurement module according to a predetermined amount, where the measurement channel is determined according to a predetermined rotation sequence, so that one of the plurality of measurement containers in the C-reactive protein measurement module obtains different distribution samples according to the predetermined rotation sequence.

The following describes the blood routine and measurement of parameters of C-reactive protein in a specific configuration of the C-reactive protein measurement module.

As shown in fig. 1, the C-reactive protein measuring module includes two measuring channels 21 and 22, wherein the schematic composition of one measuring channel is shown in fig. 3, and the C-reactive protein measuring module mainly includes a reaction vessel 221, a sample transporting pipeline 222, a measuring vessel 223, a detecting device, a CRP measuring cell waste liquid discharging mechanism 224, a reaction cell waste liquid discharging mechanism 225, and a hemolytic agent transporting pipeline 226, the reaction vessel 221 and the measuring vessel 223 are controllably communicated through the sample transporting pipeline 222, the detecting device is a photodetector, and includes a light emitting end 227 and a light detecting end 228, in this embodiment, the light emitting end 227 is a light source for emitting light capable of irradiating the measuring vessel, and the light detecting end 228 is a photoelectric sensor for receiving transmitted light passing through the measuring vessel. In the present embodiment, the light emitting end 227 and the light detecting end 228 are provided on opposite sides of the measuring container 223, respectively. The two measurement channels may be of the same construction as described above, or of different constructions, e.g. there is no reaction vessel in the other measurement channel, and the reaction and measurement of the sample and reagent is performed in the measurement vessel. It will be appreciated by those skilled in the art that scattered light passing through the measurement vessel may also be detected, and the positions of the light emitting end and the light detecting end may be adjusted as desired.

The basic working principle is as follows: after the measurement is started, the sample is placed on a sample sucking position, the sample is sucked by the sample collecting and distributing module 3, then a moving component on the sample collecting and distributing module 3 moves above each measuring module, and the required sample is distributed to corresponding measuring modules, such as a CRP measuring module 2, a WBC classifying measuring module 11, a WBC/HGB measuring module 12 and an RBC/PLT measuring module 13 respectively. After the samples are distributed, each measuring module immediately starts measuring the corresponding parameters, and after the measurement is completed, the measuring modules are cleaned to enter a standby state, and the next measurement is waited for.

Since the CRP parameter measurement time of a single sample is longer than the blood conventional parameter measurement time (2 minutes and 1 minute respectively), in order to realize a high test speed of 60 samples/hour when the two parameters are measured simultaneously, the embodiment adopts a dual-channel alternate measurement design of the CRP measurement module. The specific principle is as follows: the two separate CRP measurement channels, the CRP first measurement channel 21 and the CRP second measurement channel 22, are integrated together to form the CRP measurement module 2. In the case of continuous measurement of samples, each time a sample is collected, the collected sample is quantitatively distributed to all blood routine measuring modules and alternately to one measuring channel in the CRP measuring module, and for each blood routine measuring module, the blood routine measurement of the next sample is started after the blood routine measurement of one sample is finished. For the two measurement channels of the C-reactive protein measurement module, CRP measurement of each sample is alternately performed in the two CRP measurement channels in sequence, and the CRP measurement processes of the two distributed samples are time-overlapped, so that each sample can start blood routine measurement of the next sample without waiting for completion of CRP parameter measurement of the current sample after completion of blood routine parameter measurement. Finally, after the measurement of the CRP parameter of each sample is finished, the blood routine and the CRP parameter are output simultaneously, so that all measurement results of one sample per minute are output, the speed of the whole test is improved, and the high test speed of 60 samples/hour is achieved.

Please refer to fig. 4. Assuming that the time taken for CRP parameter measurement is 2 minutes, the time for blood routine parameter measurement is 1 minute. In fig. 4, samples 1 to 8 are samples to be measured, which are collected continuously, 0min represents the measurement start time, and 1min to 9min represents the time (min is a time unit, minutes) elapsed from the measurement start time. The blood routine parameter measurement of the 8 samples is sequentially completed in series in the blood routine measurement module 1, and each sample takes 1min. CRP parameter measurements of samples 1-8 were then performed alternately in CRP measurement channel 1 and CRP measurement channel 2, each taking 2 minutes. As shown in fig. 3, the sample collection and distribution module 3 distributes the sample 1 to the CRP first measurement channel 21 to perform CRP parameter measurement of the sample 1, then the sample collection and distribution module 3 distributes the sample 2 to the CRP second measurement channel 22 to perform CRP parameter measurement of the sample 2, then distributes the sample 3 to the CRP first measurement channel 21, then distributes the sample 4 to the CRP second measurement channel 22 to perform CRP parameter measurement, and so on, and the sample collection and distribution module 3 distributes the collected 8 samples to the two measurement channels (CRP first measurement channel 21 and CRP second measurement channel 22) in turn according to a preset sequence. In the above procedure, the blood routine parameters of each sample are measured 1min earlier than the CRP parameters, but all the measurement results of the sample are output together when the CRP parameters are completed. Once the measurement is started, the total measurement result of the first sample (sample 1) is output 2 minutes after the first sample starts to be measured (since CRP parameter measurement takes 2 minutes), and thereafter the total measurement result of one sample is output every 1 minute. Of course, all measurements of the last sample should be output t=1 min after the blood routine parameter measurement, where the value of t is the CRP parameter measurement time elapsed time minus the blood routine parameter measurement time elapsed time. From this, it can be seen that if 60 samples are measured continuously, the measurement results of all 60 samples can be output for about 60 minutes, and the measurement speed is about 60 samples/hour. It should be noted that, the above examples are merely for the convenience of understanding the technical solution by those skilled in the art, and are not to be considered as all the technical solutions, for example, the measurement channels for CRP parameter measurement may be plural, and the blood routine parameter measurement time and the CRP parameter measurement time may be other times. In one embodiment, the sample collection and distribution module 3 includes a moving mechanism and a sampling needle fixed on the moving mechanism, and the moving mechanism drives the sampling needle to move in the horizontal direction and the vertical direction. Fig. 5 is a schematic diagram illustrating an exemplary structure of the sample collection and distribution module 3 according to the present embodiment. The sample collection and distribution module 3 comprises: a fixed bracket 31, an X-direction guide rail 32, an X-direction transmission device 33, a movable bracket 34, a Z-direction guide rail 35, a Z-direction transmission mechanism 36, a sampling needle 37 and a swab 38. The fixing bracket 31 is connected with the fixing bracket of the detector, and of course, in other embodiments, the fixing bracket of the detector can be directly utilized for replacement; the movable bracket 34 is in sliding connection with the fixed bracket 31 through the X-direction guide rail 32 and the X-direction transmission part 33, so that the movable bracket 34 and the components mounted on the movable bracket can move along the X-direction to form a moving mechanism, and the power of the moving mechanism is from the X-direction transmission device 33; the sampling needle 37 is in sliding connection with the movable bracket 34 through the Z-direction guide rail 35 and the Z-direction transmission mechanism 36, so that the sampling needle 37 can move in the Z direction relative to the movable bracket 34; the swab 38 serves to clean the outer wall of the sampling needle 37, and when the sampling needle 37 moves in the Z direction, the swab 38 provides liquid to clean the outer wall of the sampling needle through the liquid path support module 8 while drawing the cleaned liquid away.

The sample collection and distribution module 3 works as follows:

(1) sample collection

The movable holder 34 is moved to the sample sucking position 49 by the driving of the X-direction driving device 33, and the sampling needle 37 is moved down into the test tube at the sample sucking position 49 by the Z-direction driving device 36. At this time, the sampling needle 37 can absorb a predetermined amount of sample stored in the sampling needle 37 by the power supplied from the liquid path support module 8, and the sample collection operation is completed.

(2) Sample post-collection cleaning

After the sample is collected, a small amount of sample is inevitably adhered to the outside of the sampling needle 37, and when the sampling needle 37 ascends, the swab 38 cleans the outer wall of the sampling needle 37, so that quantitative influence caused by the sample on the outer wall is avoided.

(3) Sample distribution

The movable bracket 34 is driven by the X-direction transmission device 33 to move above the corresponding measuring module; the sampling needle 37 is moved down into the measuring module by means of the Z-direction drive 36. When the needle tip of the sampling needle reaches the inside of the measuring module, the liquid path supporting module 8 provides power to quantitatively push out the sample stored in the sampling needle 37, and the sample is added into the measuring module to complete the sample distribution action.

It should be noted that, the above Z direction is a vertical direction, and the X direction is a horizontal direction, in other embodiments, the horizontal direction may be a Y direction, or the X direction and the Y direction may be, for example, a transmission rail is added in the X direction and the Y direction, and a Y direction transmission device is added to enable the movement of the moving mechanism (such as the movable bracket 34) in the X direction and the Y direction; for another example, the X-direction transmission 33 may be replaced by a rotation device that rotates in a horizontal plane.

In a preferred embodiment, each blood routine measurement module 1, C-reactive protein measurement module 2 and sample aspiration site 49 are arranged along the horizontal movement trajectory of the sampling needle 37. The sample sucking position 49 is preferably provided at a position near the start end of the horizontal movement path of the sampling needle.

In the preferred embodiment, the sample collection and distribution module 3 collects a sample once and then distributes it in sections to each of the blood routine measurement module 1 and the C-reactive protein measurement module 2. Referring to fig. 6a and 6b, since the sample amount required for measurement of each item in the blood routine measurement module 1 and the C-reactive protein measurement module 2 is determined, the sample collection and distribution module 3 can complete the collection at one time according to the sample amount required for measurement of each module. Assuming that in one clinical test, the blood routine parameter measurement needs to measure two items (such as WBC classification item and WBC/HGB measurement item), the sample size is V1 and V2, respectively, and the sample size is V3, which is needed for CRP parameter measurement, the sample size collected by the sample collection and distribution module 3 at one time is greater than or equal to v1+v2+v3, as shown in fig. 6 a. The sample collection and distribution module 3 then distributes the sample amounts required by the blood routine measurement module 1 and the C-reactive protein measurement module 2 to the respective measurement containers. For example, a section of sample with the quantity V1 is allocated to the measurement container of the WBC classification item, and the sample collection and allocation module 3 still leaves a sample of v2+v3, as shown in fig. 6 b; the sample collection and distribution module 3 distributes the remaining two segments of samples of v2+v3 to the WBC/HGB measurement items and to the measurement containers for CRP parameter measurement, respectively. In other embodiments, the number of segments may be divided into more segments or reduced according to the needs of the measurement item. The sample distribution method has the advantages that the samples are not required to be collected successively and distributed into each measuring container, the time is saved compared with the mode of collecting the samples for many times by the mode of collecting the samples at one time, and the measuring efficiency is improved. Further, to avoid cross-contamination of samples dispensed into different measurement receptacles, a predetermined volume of discarded sample is provided between the two sample segments. After the sample contacted with the reagent is discarded, the section of sample can be prevented from influencing the measurement result of the next measurement module, and the samples used by two adjacent measurement modules are ensured to have no cross contamination.

When CRP measurement is carried out, the hemolytic agent is added into the CRP reaction container through the hemolytic agent conveying pipeline under the drive of the liquid path supporting module 8, the hemolytic agent reacts with blood samples and latex reagents which are added later in the CRP reaction container, then the sample is conveyed into the CRP measurement container through the sample conveying pipeline, and the light emitted by the light emitting end and emitted by the CRP measurement container and the sample liquid is detected by the light detecting end; after the reaction vessel and the measurement vessel are operated, the waste liquid is respectively discharged from the CRP reaction vessel and the CRP measurement vessel by the waste liquid discharge mechanism of the reaction vessel and the waste liquid discharge mechanism of the measurement vessel under the drive of the liquid path support module. When the hemolytic agent is conveyed into the reaction container, unlike the scheme of adopting the sampling needle to convey in the prior art, the hemolytic agent is added into the reaction container by adopting a special hemolytic agent conveying pipeline in the embodiment, so that the purpose is to save the time occupied by the sampling needle for sucking and distributing the reagent, improve the measurement speed of CRP, and further improve the overall test speed.

Each measurement module is cleaned after the measurement is completed and before the next sample measurement is started.

In other embodiments, multiple measurement channels in the C-reactive protein measurement module may share a reaction vessel that controllably communicates with different measurement vessels through different sample transport lines. The detection means may also be shared, for example, an optional ring mechanism is provided in the C-reactive protein measuring module, and a plurality of measuring vessels are arranged in a row on the ring mechanism, in which case there may be only one detection means in the C-reactive protein measuring module, which detection means is provided in the rotation path of the ring mechanism, the measuring vessels being rotated with the ring mechanism, passing the detection means one by one and stopping for detection, the detection means detecting the reaction liquid in the measuring vessels stopped in the detection area thereof.

According to the blood detector disclosed by the embodiment, the plurality of measuring channels are arranged in the C-reactive protein measuring module, so that when the whole blood sample is used for carrying out conventional blood cell detection and CRP detection by a single machine, the waiting time for C-reactive protein measurement is effectively utilized for carrying out conventional blood cell detection of other samples, and therefore, the conventional blood parameter measurement and the C-reactive protein measurement of each sample can be cooperated, the conventional blood parameter measurement and the C-reactive protein measurement time are comprehensively planned, and the measurement speed is improved.

Example 2:

unlike the above embodiments, the blood detector disclosed in this embodiment further includes an auto-sampling module 4, as shown in fig. 1, where the auto-sampling module 4 provides continuous samples to the sample collection and distribution module 3 and completes sample loading and unloading, and the auto-sampling module 4 is preferably disposed at the front end of the blood detector. Please refer to fig. 7, which is a schematic top view of the auto-sampling module, mainly comprising: a rack conveying mechanism 41, a loading position detecting mechanism 42, a rack loading mechanism 43, a rack unloading mechanism 44, a test tube presence detecting mechanism 45, and a test tube barcode information acquiring mechanism 46. The working process is as follows: the tube rack conveying section 41 conveys the tube rack with the test tube placed therein to the loading area 410 in the X direction, and when the loading position detecting mechanism 42 detects that the tube rack is in place, the tube rack loading mechanism 43 sequentially moves the tube rack in the Y direction along the tube position on the tube rack into the test tube detecting position 47, the sample mixing position 48, and the sample sucking position 49; when each test tube placement position on the test tube rack reaches the test tube detection position 47, the test tube presence detection mechanism 45 detects whether a test tube exists at the position, and the test tube bar code information acquisition mechanism 46 scans the bar code on the test tube; if the test tube is detected, when the test tube at the position moves to a 48 sample mixing position, a mixing module in the device completes mixing of the test tube, and then when the test tube moves to a sample sucking position 49, a sample collecting and distributing module 3 sucks the sample; when the last tube position of the whole tube rack is moved out of the sample sucking position 49, the tube rack unloading mechanism 44 pushes the tube rack into the unloading area 411 in the opposite direction of the X direction, and the unloading of the whole tube rack sample is completed.

In summary, the working flow of the whole automatic sample feeding module 4 is as follows:

(1) placing a test tube and a test tube rack, and starting an automatic sample injection program;

(2) conveying the test tube to a loading position along with a test tube rack;

(3) loading a test tube rack, and sequentially moving the test tube into a test tube detection position 47, a sample mixing position 48 and a sample sucking position 49;

(4) detecting whether a test tube exists or not in the test tube detection bit 47, and scanning the test tube bar code when the test tube exists;

(5) at sample mixing position 48, if test tubes are present, sample mixing is performed, otherwise, sample sucking position 49 is directly shifted;

(6) at sample pick-up location 49: if the test tube exists, sample suction is carried out;

(7) and unloading the test tube rack if the current sample is positioned at the last test tube position of the current test tube rack.

In one embodiment, the sample pick-up location 49 should preferably be located at the beginning of the horizontal movement path of the sampling needle.

In a preferred embodiment, the X-direction of the autosampler module 4 should coincide with the X-direction of the sample acquisition and distribution module 3.

The blood detector disclosed in this embodiment improves the automation degree of the blood detector by adding the automatic sample injection module 4, and is more beneficial to the management of samples (especially the number of samples is various), thereby further improving the blood routine of the whole blood sample and the overall measurement speed of CRP parameters.

Example 3:

unlike the above embodiments, the blood test apparatus disclosed in this embodiment further includes a latex reagent storage module 5, as shown in fig. 1, the latex reagent storage module 5 is configured to provide a low-temperature preservation environment for the latex reagent, and the latex reagent storage module 5 is disposed at a position of the blood test apparatus closer to an edge of the test apparatus and further from the inside. The latex reagent storage module 5 is arranged at a position far away from the inside of the detector, so that latex reagent can be conveniently replaced, and when the latex reagent is replaced, the user can be prevented from stretching hands into the inside of the detector, and the risk of biological pollution to the user is reduced.

In a preferred embodiment, referring to fig. 7, a latex reagent storage module 5 may be disposed between the sample loading area 410 and the sample unloading area 411 of the autosampler module 4 to facilitate sharing of the latex reagent and the sample with the sampling needle and to simplify the movement stroke of the sampling needle.

In one embodiment, referring to fig. 8, the latex reagent storage module 5 comprises: a refrigeration mechanism 51 and a cold room door 52.

The refrigeration mechanism 51 has a refrigeration chamber 53 inside and an opening 54 on the side for providing a low temperature for the latex reagent.

The cold chamber door 52 is used for closing the cold chamber from the side opening of the cold chamber, one surface of the cold chamber door facing the cold chamber is provided with a latex reagent placing position 50, and the cold chamber door can expose the latex reagent placing position outside the measuring instrument or close the latex reagent placing position to the cold chamber under the stress state.

In a specific embodiment, the cold room door and the refrigerating mechanism are of split type, and the cold room door can be far away from the refrigerating room and exposed outside the measuring instrument or close to the refrigerating room and close the refrigerating room by the outside of the measuring instrument in a push-pull and/or overturning mode. For example, the cold room door 52 may be far away from the cold room and exposed to the exterior of the meter in a stressed state, where the cold room door 52 is open, thereby facilitating user access to the latex reagent placement site 50; the cold chamber door 52 is forced by opposing forces from outside the meter to close and enclose the cold chamber, with the latex reagent placement site 50 within the cold chamber cavity.

In a preferred embodiment, the blood test apparatus may further comprise an emergency sample placement site 55, the emergency sample placement site 55 being provided on a side of the cold room door 52 facing away from the cold room. At this time, the emergency sample placement site 55 is exposed to the outside of the blood test apparatus prior to the latex reagent placement site 50, and the design is used to facilitate the measurement of the emergency sample because the frequency of adding the emergency sample to the blood test apparatus is higher than that of adding/replacing the latex reagent to the blood test apparatus in clinical tests.

In the following, taking two consecutive samples (sample 1 and sample 2) for simultaneously measuring the blood normal parameter and the CRP parameter of the whole blood sample as an example, the blood normal measurement module includes a WBC classification measurement module, a WBC/HGB measurement module and a RBC/PLT measurement module, please refer to fig. 9, the flow is as follows:

and step 1, starting measurement, and automatically sampling and uniformly mixing the sample 1. After the measurement is started, the automatic sample injection module 4 completes sample injection of the sample 1, test tube presence detection, test tube bar code information acquisition and sample mixing according to the working procedure described in the embodiment 2.

And 2, sucking the sample 1. When the test tube reaches the sample sucking position 49, the sample collecting and distributing module 3 is driven by the X-direction transmission device 33, the moving mechanism (such as the movable bracket 34) is moved to the sample sucking position 49, the sampling needle 37 is moved downwards into the test tube at the sample sucking position 49 by the Z-direction transmission mechanism 36, and the sample required for measuring the blood routine parameters and CRP parameters is sucked into the sampling needle 37 once. The sampling needle is raised to an initial height by the drive of the Z-direction drive mechanism 36 while the swab 38 cleans the outer wall of the sampling needle 37.

And 3, adding a CRP hemolytic agent into the CRP measurement channel 1. The liquid path support module 8 provides power to add CRP hemolytic agent into the CRP measurement channel 1 in the C-reactive protein measurement module 2.

And 4, separating blood from the CRP measurement channel 1. Driven by the X-direction drive 33, the movable support 34 is moved over the CRP measurement channel 1, and the sampling needle 37 is moved down into the CRP measurement channel 1 by the Z-direction drive 36, and the blood sample required for CRP measurement is added. Sample hemolysis is initiated immediately after the blood sample is added to CRP measurement channel 1, ready for subsequent CRP measurements. The sampling needle is raised to an initial height by the drive of the Z-direction drive mechanism 36 while the swab 38 cleans the outer wall of the sampling needle 37.

And 5, separating blood by the WBC classification measurement module. Driven by the X-direction drive 33, the movable support 34 moves over the WBC classification measurement module 11, and the sampling needle 37 is moved down into the WBC classification measurement module 11 by the Z-direction drive 36 to perform blood separation and initiate WBC classification measurement. After the blood separation is completed, the sampling needle 37 is lifted to an initial height under the drive of the Z-direction transmission mechanism 36, and the swab 38 cleans the outer wall of the sampling needle 37.

And 6, separating blood by the WBC/HGB measurement module. Driven by the X-direction drive 33, the movable carriage 34 moves over the WBC/HGB measurement module 12, moving the sampling needle 37 down into the WBC/HGB measurement module 12 through the Z-direction drive 36, dispensing the module and RBC/PLT measurement module 13 into which the desired blood sample is measured.

Step 7, the diluted sample in the WBC/HGB measurement module 12 is sucked and distributed to the RBC/PLT measurement module 13. After the blood sample is diluted, the fluid circuit support module 8 provides the motive force to draw a portion of the diluted sample from the WBC/HGB measurement module 12 into the sampling needle 37. The sampling needle 37 is raised to an initial height under the drive of the Z-direction drive mechanism 36, then the X-direction drive mechanism 33 drives the movable support 34 to move above the RBC/PLT measurement module 13, and the sampling needle 37 is moved down into the RBC/PLT measurement module 13 by the Z-direction drive mechanism 36 to perform blood separation and initiate RBC and PLT measurement. After the blood separation is completed, the sampling needle 37 is lifted to an initial height under the drive of the Z-direction transmission mechanism 36, and the swab 38 cleans the outer wall of the sampling needle 37.

Step 8, adding a hemolytic agent into the WBC/HGB measurement module 12. The fluid circuit support module 8 adds a hemolytic agent to the WBC/HGB measurement module 12, initiating WBC and HGB measurements.

And 9, sucking the latex reagent. The X-direction transmission device 33 drives the movable support 34 to move to the position above the latex reagent storage module 5, and the sampling needle 37 is moved downwards into the latex reagent storage module 5 through the Z-direction transmission mechanism 36 to suck the latex reagent into the sampling needle 37. The sampling needle 37 is raised to an initial height by the drive of the Z-direction drive mechanism 36 while the swab 38 cleans the outer wall of the sampling needle 37.

Step 10, latex reagent is added into the CRP measurement channel 1. The X-direction drive 33 drives the movable support 34 to move over the C-reactive protein measurement module 2, moving the sampling needle 37 down into the CRP measurement channel 1 via the Z-direction drive 36 to add latex reagent thereto while the CRP measurement is started.

Step 11, wash WBC classification measurement module 11, WBC/HGB measurement module 12, and RBC/PLT measurement module 13. After the WBC classification measurement module 11, WBC/HGB measurement module 12 and RBC/PLT measurement module 13 complete the respective measurements of the sample 1, the fluid path support module 8 conveys the reagent into the corresponding measurement module and completes the cleaning.

Step 12, sample 2 is automatically sampled and mixed uniformly, and the sample 2 is sucked. Repeating the steps 1 and 2, and processing the sample 2.

And 13, adding a CRP hemolytic agent into the CRP measurement channel 2. The liquid path support module 8 provides power to add the CRP hemolytic agent into the CRP measurement channel 2 of the C-reactive protein measurement module 2.

Step 14, CRP measuring channel 2 blood separation. The X-direction actuator 33 drives the movable support 34 to move over the CRP measurement channel 2, and the sampling needle 37 is moved down into the CRP measurement channel 2 by the Z-direction actuator 36, and a blood sample required for CRP measurement is added. Sample hemolysis begins immediately after the blood sample is added to CRP measurement channel 2, ready for subsequent CRP measurements. The sampling needle 37 is raised to an initial height by the Z-drive 36 while the swab 38 cleans the outer wall of the sampling needle 37.

Step 15, conventional measurement of sample 2 blood and sucking of latex reagent. And repeating the steps 5 to 9.

Step 16, latex reagent is added into the CRP measurement channel 2. The X-direction drive 33 drives the movable support 34 to move over the C-reactive protein measurement module 2, moving the sampling needle 37 down into the CRP measurement channel 2 via the Z-direction drive 36 to add latex reagent thereto while the CRP measurement is started.

Step 17, cleaning WBC classification measurement module 11, WBC/HGB measurement module 12, and RBC/PLT measurement module 13. After completion of the routine measurement of the blood of sample 2, step 11 is repeated.

Step 18, cleaning the C-reactive protein measurement module 2. Since the CRP measurement time is longer than the blood routine measurement time, the CRP measurement is completed by the sample 1 in the CRP channel 1 after waiting until the blood routine of the sample 2 is completed, and at this time, the liquid path support module 8 starts the washing of the CRP measurement channel 1. After 1 minute again, the liquid path support module 8 starts the cleaning of the CRP measurement channel 2 until the CRP measurement channel 2 completes the measurement of the sample 2.

Thus, measurement of the whole blood sample blood conventional parameter and CRP parameter of each of the two consecutive samples is completed.

The foregoing description of specific examples has been presented only to aid in the understanding of the present application and is not intended to limit the present application. Variations of the above embodiments may be made by those of ordinary skill in the art in light of the concepts of the present application.

Claims

1. A method of detecting blood routine parameters and C-reactive protein parameters in a blood sample, the method comprising:

providing a blood detector with a blood routine measurement module, a C-reactive protein measurement module, a sample collection and distribution module and an automatic sample injection module, wherein the automatic sample injection module automatically provides continuous samples for the sample collection and distribution module and completes sample loading and unloading, and the automatic sample injection module is provided with a test tube rack conveying mechanism, a loading position detection mechanism, a test tube rack loading mechanism and a test tube rack unloading mechanism; the C-reactive protein measurement module comprises a reaction container, a measurement container, a sample conveying pipeline, a CRP measurement container waste liquid discharge mechanism and a reaction container waste liquid discharge mechanism, wherein the reaction container and the measurement container are controllably communicated through the sample conveying pipeline, and the measurement container is used for providing a measurement place for the reaction liquid; the CRP measuring container waste liquid discharging mechanism is communicated with the measuring container and is used for discharging waste liquid in the measuring container, and the reaction container waste liquid discharging mechanism is communicated with the reaction container and is used for discharging waste liquid in the reaction container;

the test tube rack conveying mechanism conveys test tube racks with test tubes placed to a loading area;

The loading position detection mechanism detects that the test tube rack is in place;

the test tube rack loading mechanism moves the test tube rack to a sample mixing position and uniformly mixes at least one test tube placed on the test tube rack;

the sample collection and distribution module absorbs the uniformly mixed sample in the test tube;

the blood routine measurement module and the C-reactive protein measurement module detect the sucked sample to obtain at least one blood routine parameter and a C-reactive protein parameter, and in the continuous detection process, after the reaction container finishes the operation and the measurement container does not finish the measurement of the C-reactive protein parameter of the current sample, waste liquid in the reaction container is discharged by the reaction container waste liquid discharge mechanism; and/or after the measurement vessel has completed the operation and the reaction vessel has not completed the reaction of the next whole blood sample, the waste liquid in the measurement vessel is discharged by the CRP measurement vessel waste liquid discharge mechanism; and

and the test tube rack unloading mechanism pushes the test tube rack subjected to detection into an unloading area to finish unloading of the test tube rack.

2. The method of claim 1, wherein the sample collection and distribution module collects a sample amount greater than or equal to a sum of sample amounts required by the respective measurement modules at a time, and distributes the collected samples to the respective measurement modules in a predetermined order.

3. The method of claim 1, wherein the blood routine measurement module comprises a WBC classification measurement module and a WBC/HGB measurement module: the method may further comprise the steps of,

the aspirated samples are distributed to the modules in the order of the C-reactive protein measurement module, the WBC class measurement module, and the WBC/HGB measurement module.

4. The method of claim 3, wherein the blood routine measurement module further comprises an RBC/PLT measurement module, the method further comprising aspirating diluted samples from the WBC/HGB measurement module for distribution to the RBC/PLT measurement module.

5. The method of claim 1, wherein the blood routine measurement module comprises a WBC classification measurement module and a WBC/HGB measurement module, the sample collection and distribution module discarding a predetermined volume of whole blood sample within a sampling needle after injecting the whole blood sample into the WBC classification measurement module and before distributing the whole blood sample to the WBC/HGB measurement module; or the sample collection and distribution module discards a predetermined volume of whole blood sample in the sampling needle after injecting the whole blood sample into the WBC/HGB measurement module and before distributing the whole blood sample to the WBC classification measurement module.

6. The method of claim 1, wherein the sample collection and dispensing module discards a predetermined volume of sample within a sampling needle after injecting a whole blood sample into the C-reactive protein measurement module and prior to dispensing a whole blood sample into the blood routine measurement module.

7. The method of claim 1, wherein the blood test apparatus further comprises a fluid circuit support module that provides power to add a hemolytic agent to the C-reactive protein measurement module via a conduit.

8. The method of claim 1, wherein the blood analyzer further has a fluid path support module; the C-reactive protein measurement module comprises a plurality of measurement channels formed by a reaction container and a measurement container; the method comprises the following steps:

the sample collection and distribution module distributes a whole blood sample to the blood routine measurement module and a reaction container in a first measurement channel in the C-reactive protein measurement module;

the liquid path support module provides power to add a hemolytic agent into the reaction container in the first measuring channel, the hemolytic agent reacts with the whole blood sample and the latex reagent in the reaction container in the first measuring channel, and then the reacted solution is conveyed into the measuring container in the first measuring channel of the C-reactive protein measuring module;

When the blood routine measurement module ends at least one blood routine parameter measurement of the current sample and the current sample does not complete the C-reactive protein parameter measurement in the first measurement channel of the C-reactive protein measurement module, the sample collection and distribution module begins to distribute the collected next whole blood sample to the reaction vessel in the second measurement channel of the C-reactive protein measurement module and the blood routine measurement module.

9. The method of claim 8, wherein the washing of the first measurement channel is initiated when the second measurement channel does not complete the measurement of the C-reactive protein parameter.

10. The method of claim 9, wherein the fluid circuit support module cleans the measurement vessel of the C-reactive protein measurement module after the current sample has completed the C-reactive protein parameter measurement.

11. The method of claim 1, wherein the sample collection and dispensing module moves the sampling needle down into the reaction vessel of the C-reactive protein measurement module to inject the whole blood sample while the sample collection and dispensing module dispenses the sucked sample to the C-reactive protein measurement module and then to the blood routine measurement module, and discards a sample of a preset volume within the sampling needle of the sample collection and dispensing module after injecting the whole blood sample into the reaction vessel of the C-reactive protein measurement module and before dispensing the whole blood sample to the blood routine measurement module.

12. A method of detecting blood routine parameters and C-reactive protein parameters in a blood sample, the method comprising:

providing a blood detector with a blood routine measurement module, a C-reactive protein measurement module, a sample collection and distribution module and a liquid path support module, wherein the C-reactive protein measurement module comprises a reaction container, a measurement container, a sample conveying pipeline, a CRP measurement container waste liquid discharge mechanism and a reaction container waste liquid discharge mechanism, the reaction container and the measurement container are controllably communicated through the sample conveying pipeline, and the measurement container is used for providing a measurement place for a reaction liquid; the CRP measuring container waste liquid discharging mechanism is communicated with the measuring container and is used for discharging waste liquid in the measuring container, and the reaction container waste liquid discharging mechanism is communicated with the reaction container and is used for discharging waste liquid in the reaction container;

after the reaction vessel finishes the operation and the measurement vessel does not finish the measurement of the C-reactive protein parameter of the current sample, the waste liquid in the reaction vessel is discharged by the reaction vessel waste liquid discharge mechanism; and/or the waste liquid in the measuring container is discharged by the CRP measuring container waste liquid discharging mechanism after the measuring container finishes the operation and when the reaction container does not complete the reaction of the next whole blood sample.

13. The method as recited in claim 12, further comprising:

14. A blood test apparatus, comprising:

A C-reactive protein measurement module for providing a measurement site for the dispensed sample, performing measurement on the dispensed whole blood sample for the purpose of obtaining C-reactive protein parameters and outputting a measurement result, the C-reactive protein measurement module comprising a reaction vessel, a measurement vessel, a sample transport line, a CRP measurement vessel waste liquid discharge mechanism, a reaction vessel waste liquid discharge mechanism, and a hemolysis agent transport line for adding a hemolysis agent into the reaction vessel, the reaction vessel further receiving the whole blood sample and the latex reagent dispensed by the sample collection and dispensing module, the reaction vessel and the measurement vessel being controllably communicated through the sample transport line, the measurement vessel for providing a measurement site for the reaction liquid; the CRP measuring container waste liquid discharging mechanism is communicated with the measuring container and used for discharging waste liquid in the measuring container, and the reaction container waste liquid discharging mechanism is communicated with the reaction container and used for discharging waste liquid in the reaction container;

the control and information processing module is used for controlling the C-reactive protein measuring module to discharge waste liquid in the reaction container by the reaction container waste liquid discharge mechanism after the reaction container finishes operation and when the measurement container does not finish measurement of the C-reactive protein parameter of the current sample; and/or the waste liquid in the measuring container is discharged by the CRP measuring container waste liquid discharging mechanism after the measuring container finishes the operation and when the reaction container does not complete the reaction of the next whole blood sample.