CN109932519A - Blood test device - Google Patents

Blood test device Download PDF

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Publication number
CN109932519A
CN109932519A CN201910319409.2A CN201910319409A CN109932519A CN 109932519 A CN109932519 A CN 109932519A CN 201910319409 A CN201910319409 A CN 201910319409A CN 109932519 A CN109932519 A CN 109932519A
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China
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pond
crp
wbc
saa
reagent
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CN201910319409.2A
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Chinese (zh)
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甘泉
陈新基
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Edan Instruments Inc
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Edan Instruments Inc
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Priority to CN201910319409.2A priority Critical patent/CN109932519A/en
Publication of CN109932519A publication Critical patent/CN109932519A/en
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Abstract

The invention discloses a kind of blood test device, the blood test device sampling needles, counting cell unit;The sampling needle is for drawing and distributing blood sample to the counting cell unit;The counting cell unit includes the pond CRP, the pond WBC, the pond RBC, and the pond CRP measures CRP parameter as dilution, the reaction vessel of CRP reagent and blood sample;The pond WBC measures WBC quantity as dilution, the reaction vessel of WBC reagent and blood sample;The pond RBC is counted for carrying out RBC and PLT.The present invention can do the measurement of blood routine parameter and the measurement of CRP simultaneously, realization automatic measurement blood routine parameter and CRP parameter on an instrument with a venous whole, to provide more fully diagnostic data in lower cost and shorter time for inflammation, hospital outpatient efficiency is improved.

Description

Blood test device
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of blood test devices.
Background technique
In clinic, being frequently run onto some patients' needs while detecting blood routine and certain Special Proteins to diagnose the patient is It is no since bacterium or virus infection bring slight illness, the detection of Special Proteins generally comprises CRP, and (C-reactionprotein, C are anti- Answer albumen) measurement and SAA (serum amyloid A protein) measurement, blood routine detection and blood in Special Proteins detection due to Using different detection methods, so being completed by different instruments.
Due to detect different types of haemocyte or Special Proteins, it is therefore desirable to which purchasing different detecting instruments could be complete At detection, the equipment input cost that is bound to is high, inconvenient.
Summary of the invention
The main object of the present invention is to provide a kind of blood test device, it is intended to solve detection device function in the prior art Single and inconvenient technical problem.
To achieve the above object, blood test device proposed by the present invention includes sampling needle, counting cell unit, syringe list Member, dilution allocation unit and negative pressure chamber;
The sampling needle is for drawing and distributing blood sample to the counting cell unit;
The counting cell unit includes the pond CRP, the pond WBC, the pond RBC, and the pond CRP is as dilution, CRP reagent and blood sample Reaction vessel, the pond WBC as dilution, the reaction vessel of WBC reagent and blood sample, the pond RBC for carry out RBC and PLT is counted;
The dilution allocation unit is separately connected the pond CRP, the pond WBC and the pond RBC;The syringe unit is used for will Dilution is distributed via the dilution allocation unit to the pond CRP, the pond WBC and the pond RBC;The syringe unit be also used to by CRP reagent is assigned to the pond CRP, WBC reagent is assigned to the pond WBC;
The negative pressure chamber provides sample flow driving force for the pond WBC and the pond RBC.
Preferably, the blood test device does not include the pond DIFF and DIFF lane testing unit.
Preferably, the counting cell unit further includes the pond SAA, and the pond SAA is as dilution, SAA reagent and blood sample Reaction vessel, and measure SAA parameter.
Preferably, the pond CRP includes CRP pond body, first laser device and the first photodetector, the CRP pond body Mouth, CRP waste liquid outlet is added with CRP reagent;The first laser device and first photodetector are in CRP pond body CRP measurement of concetration.
Preferably, the pond SAA includes SAA pond body, second laser and the second photodetector, the SAA pond body Mouth, SAA waste liquid outlet is added with SAA reagent;The second laser and second photodetector are in SAA pond body SAA measurement of concetration.
Preferably, the pond WBC and the pond RBC have respectively included impedance measuring unit, each impedance measurement list Member includes forebay electrode, gem hole, after-bay electrode;The negative pressure of cell in the pond WBC and the pond RBC in the negative pressure chamber One by one by the gem hole under effect, when cell passes through the gem hole, the forebay electrode can be produced with the after-bay electrode Raw pulse, calculates WBC quantity, RBC quantity and PLT quantity according to number of pulses.
Preferably, the pond WBC further includes HGB measuring unit, and the HGB measuring unit includes light emitting diode and photoelectricity Receiver, the light that the light emitting diode issues is transmitted to the photelectric receiver by the reaction solution in the pond WBC, according to described The transmitted intensity that photelectric receiver receives calculates HGB concentration.
Preferably, the blood routine detection unit further includes refrigeration unit, and the refrigeration unit is used for the SAA reagent It controls with the CRP temperature of reagent at 2-8 DEG C.
Preferably, the blood routine detection unit further includes preheating unit, and the preheating unit tries the SAA Agent, CRP reagent and dilution are preheated before being added to each reaction tank.
Blood test device proposed by the present invention can be the measurement of blood routine parameter and the measurement of CRP, Ke Yigeng simultaneously Blood routine parameter and the testing result of CRP are obtained in low cost and shorter time, provides more fully diagnostic data for inflammation; Realize with a venous whole on an instrument simultaneously automatic measurement blood routine parameter and CRP parameter, reduction measurement cost The convenience of the above parameter measurement is improved simultaneously, to improve hospital outpatient efficiency.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is the whole theory structure schematic diagram of blood test device embodiment one of the present invention;
Fig. 2 is the structural schematic diagram of dilution allocation unit in blood test device embodiment one of the present invention;
Fig. 3 is the structural schematic diagram in the pond CRP in blood test device embodiment one of the present invention;
Fig. 4 is the theory structure schematic diagram of one middle impedance measuring unit of blood test device embodiment of the present invention;
Fig. 5 is the theory structure schematic diagram of HGB measuring unit in blood test device embodiment one of the present invention;
Fig. 6 is the whole theory structure schematic diagram of blood test device embodiment two of the present invention;
Fig. 7 is the structural schematic diagram of dilution allocation unit in blood test device embodiment two of the present invention;
Fig. 8 is the structural schematic diagram in the pond SAA in blood test device embodiment two of the present invention;
Fig. 9 is the flow diagram of the blood testing of blood test device of the present invention.
Drawing reference numeral explanation:
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.Base Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its His embodiment, shall fall within the protection scope of the present invention.
It is to be appreciated that the directional instruction (such as up, down, left, right, before and after ...) of institute is only used in the embodiment of the present invention In explaining in relative positional relationship, the motion conditions etc. under a certain particular pose (as shown in the picture) between each component, if should When particular pose changes, then directionality instruction also correspondingly changes correspondingly.
In addition, the description for being such as related to " first ", " second " in the present invention is used for description purposes only, and should not be understood as Its relative importance of indication or suggestion or the quantity for implicitly indicating indicated technical characteristic.Define as a result, " first ", The feature of " second " can explicitly or implicitly include at least one of the features.In the description of the present invention, " multiple " contain Justice is at least two, such as two, three etc., unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " connection ", " fixation " etc. shall be understood in a broad sense, For example, " fixation " may be a fixed connection, it may be a detachable connection, or integral;It can be mechanical connection, be also possible to Electrical connection;It can be directly connected, the connection inside two elements or two can also be can be indirectly connected through an intermediary The interaction relationship of a element, unless otherwise restricted clearly.It for the ordinary skill in the art, can basis Concrete condition understands the concrete meaning of above-mentioned term in the present invention.
It in addition, the technical solution between each embodiment of the present invention can be combined with each other, but must be general with this field Based on logical technical staff can be realized, it will be understood that when the combination of technical solution appearance is conflicting or cannot achieve this The combination of technical solution is not present, also not the present invention claims protection scope within.
The present invention proposes a kind of blood test device.
Referring to Fig. 1, in embodiments of the present invention, which includes sampling needle 10, reagent unit 20, counts Pool unit 30, syringe unit 40, dilution allocation unit 50 and negative pressure chamber 60;
The sampling needle 10 is for drawing and distributing blood sample to the counting cell unit 30;
The reagent unit 20 includes: dilution 21, WBC reagent 23, CRP reagent 22
The counting cell unit 30 includes CRP (C-reactionprotein, c reactive protein) pond 33, WBC (White Blood Cell, leucocyte) pond 31, RBC (Red Blood Cell, red blood cell) pond 32, the pond CRP 33 is used as dilution 21, the reaction vessel of CRP reagent 22 and blood sample, and measure CRP parameter;The pond WBC 31 is used as dilution, WBC reagent 23 With the reaction vessel of blood sample, and WBC quantity is measured;The pond RBC 32 is for carrying out RBC and PLT (Platelet, blood platelet) It counts;
The dilution allocation unit 50 is separately connected the pond CRP 33, the pond WBC 31 and the pond RBC 32;The syringe list Member 40 via the dilution allocation unit 50 for distributing dilution 21 to the pond CRP 33, the pond WBC31 and the pond RBC 32;It is described Syringe unit 40 is also used to for CRP reagent 22 to be assigned to the pond CRP 33, WBC reagent 23 is assigned to the pond WBC 31;
The negative pressure chamber 60 is that the pond WBC 31 and the pond RBC 32 provide sample flow driving force.
Blood test device proposed by the present invention can be the measurement of blood routine parameter and the measurement of CRP, Ke Yigeng simultaneously Blood routine parameter and the testing result of CRP are obtained in low cost and shorter time, provides more fully diagnostic data for inflammation; Realize with a venous whole on an instrument simultaneously automatic measurement blood routine parameter and CRP parameter, reduction measurement cost The convenience of the above parameter measurement is improved simultaneously, to improve hospital outpatient efficiency.This is also some to need to do blood inspection simultaneously The Anemic patients of survey or the patient of other anaematopoiesis bring convenience, they only need to extract when doing blood testing One pipe blood sample goes different detecting instruments to detect without extracting multitube blood sample again.
Specifically, the blood test device does not include the pond DIFF and DIFF lane testing unit.It can be understood that The blood test device is the measurement for doing three sorting parameters in the measurement of blood routine.
Specifically, referring to Fig. 1, the blood test device further includes discharging of waste liquid unit 70, the waste liquid row Put discharge of the unit 70 for complete machine waste liquid.Since the discharge of pharmaceutical reagent will meet professional standard, increase discharging of waste liquid Unit 70.But for instrument and equipment, discharging of waste liquid unit 70 is also possible to be arranged in the component in other equipment, or extraneous Individual component.
Specifically, the syringe unit 40 includes the first syringe 41, the second syringe 42 and third syringe 43; First syringe 41 connects the dilution allocation unit 50, and the distribution for the dilution 21 provides power;Described Two syringes 42 are one drag two syringe, and one of syringe connects the absorption and injection that the sampling needle 10 carries out blood sample, Another syringe connects the pond WBC 31, and cooperates the first triple valve 311 that the WBC reagent 23 is assigned to the WBC Pond 31;The third syringe 43 is one drag two syringe, and the CRP reagent 22 includes the first CRP reagent 221 and the 2nd CRP Reagent 222, the third syringe 43 connect the pond CRP 33, and the second triple valve 335, third triple valve 336 is cooperated to distinguish The first CRP reagent 221 and the 2nd CRP reagent 222 are assigned to the pond CRP 33.
Specifically, referring to Figure 2 together, the dilution allocation unit 50 includes liquid storage tank 51, the 6th triple valve 52, the Seven triple valves 53 and the 8th triple valve 54;For storing the dilution 21, the dilution 21 passes through the liquid storage tank 51 6th triple valve 52, the 7th triple valve 53 and the 8th triple valve 54 are added separately to the pond CRP 33, the pond WBC 31, the pond RBC 32。
Specifically, also referring to Fig. 3, the pond CRP 33 includes CRP pond body 331, first laser device 338 and first There is CRP reagent mouth 332, CRP waste liquid outlet 337 is added for photodetector 339, the CRP pond body 331;The CRP reagent adds Entrance 332 includes that mouth 333 and the 2nd CRP reagent addition mouth 334 is added in the first CRP reagent;The first laser device 338 with it is described First photodetector 339 is separately positioned on 331 two sides of CRP pond body.As blood sample, the first CRP reagent 221 and the 2nd CRP Solution starts to react after reagent 222 is added separately in CRP pond body 331, insoluble compound occurs, when first laser device 338 The light of sending scatters when passing through insoluble compound, and scattered light intensity and CRP concentration have certain proportionate relationship, passes through The scattered light intensity that first photodetector 339 receives can calculate CRP concentration.
Specifically, referring to Figure 4 together, the pond WBC 31 and the pond RBC 32 include impedance measuring unit 312, institute Stating impedance measuring unit 312 includes forebay electrode 313, gem hole 314, after-bay electrode 315;The pond WBC 31 and the pond RBC Cell in 32, one by one by the gem hole 314, is put under the suction function of the negative pressure chamber 60 at the both ends of gem hole 314 A pair can provide the forebay electrode 313 and after-bay electrode 315 of constant current, when in sample cell or blood platelet flow through gem hole When 314, the D.C. resistance between two electrodes can change, and the voltage at electrode both ends can also change at this time, will form Pulse relevant to cellular morphology, the number of pulse are exactly the number of cell, and the size of pulse amplitude represents cell amplitude Size, so as to calculate WBC quantity, RBC quantity and PLT quantity.
Further, referring to Fig. 5, the pond WBC 31 further includes HGB (Hemoglobin, hemoglobin) measuring unit 316, the HGB measuring unit 316 includes light emitting diode 317 and photelectric receiver 318;Sample passes through hemolytic agent in measurement Afterwards, red blood cell is dissolved, HGB is reacted with hemolytic agent, forms haemoglobin complexes, there is specific wavelength in the one end in the pond WBC 31 Light emitting diode 317, other side photelectric receiver 318 carry out photoelectric conversion to transmitted light and obtain voltage signal;It allows first only The sample of dilution 21 enters the pond WBC 31, measures a background voltage, and sample is then entered the pond WBC 31 and obtains sample at this time Voltage, wherein hemoglobin concentration is bigger, and the translucency of reactant is poorer, and the voltage value of photoelectric conversion is lower, so as to Calculate HGB concentration.
Embodiment two:
Fig. 6 and Fig. 7 are please referred to, the second embodiment of the present invention is identical with the first embodiment substantially, and difference is: the meter Number pool unit 30 further includes SAA (serum amyloid A protein) pond 34, and reagent unit 20 further includes SAA reagent 24, the SAA examination Agent 24 includes the first SAA reagent 241 and the 2nd SAA reagent 242;The sampling needle 10 is for drawing and distributing blood sample described in The pond SAA 34, the pond SAA 34 measure SAA parameter as dilution 21, the reaction vessel of SAA reagent 24 and blood sample;The Three syringes 43 drag four syringes for one, and third syringe 43 connects the pond CRP 33 and the pond SAA 34, and cooperates second The first CRP reagent 221 and the 2nd CRP reagent 222 are assigned to the CRP respectively by triple valve 335, third triple valve 336 Pond 33, the 4th triple valve 345 of cooperation, the 5th triple valve 346 are respectively by the first SAA reagent 241 and the 2nd SAA reagent 242 It is assigned to the pond SAA 34;Dilution allocation unit 50 further includes the 9th triple valve 55, and the dilution 21 passes through the 9th port valve 55 are added to the pond SAA 34.
Specifically, referring to Fig. 8, the pond SAA 34 includes SAA pond body 341, second laser 348 and the second photoelectricity There is SAA reagent mouth 342, SAA waste liquid outlet 347 is added for detector 349, the SAA pond body 341, and mouth is added in the SAA reagent 342 include that mouth 343 and the 2nd SAA reagent addition mouth 344 is added in the first SAA reagent;The second laser 348 and described second Photodetector 349 is separately positioned on 341 two sides of SAA pond body.When blood sample, the first SAA reagent 241 and the 2nd SAA reagent 242, which are added separately in SAA pond body 341 rear solution, starts to react, and insoluble compound occurs, when second laser 348 issues Light scatter when passing through insoluble compound, scattered light intensity and SAA concentration have certain proportionate relationship, pass through second The scattered light intensity that photodetector 349 receives can calculate SAA concentration.
Based on above-mentioned two embodiments, the blood test device further includes refrigeration unit 80 and preheating unit 90, institute Refrigeration unit 80 is stated for the first CRP reagent 221 and the control of 241 temperature of the first SAA reagent at 2-8 DEG C, to be guaranteed reagent Validity.The preheating unit 90 be used for the first CRP reagent 221, the 2nd CRP reagent 222, the first SAA reagent 241, 2nd SAA reagent 242 and dilution 21 are preheated, and the reaction of each reaction tank is accelerated, and improve measurement efficiency.
Referring to Fig. 9, being based on above-mentioned blood test device, the present invention provides a kind of blood testing, specific method stream Journey is as follows:
Step S1: sampling needle 10 draws whole blood sample;
Step S2: sampling needle 10 is moved to 33 position of the pond CRP, injects appropriate blood sample, while third syringe 43 will pass through Preheating CRP reagent 22 is injected in the pond CRP 33 and is reacted;
Step S3: sampling needle 10 is moved to 34 position of the pond SAA, injects appropriate blood sample, while third syringe 43 will pass through Preheating SAA reagent 24 is injected in the pond SAA 34 and is reacted;
Appropriate dilution 21 is injected in the pond step S4:WBC 31, and sampling needle 10 is moved to 31 position of the pond WBC, injects appropriate blood Sample;
Step S5: sampling needle 10 draws suitable diluted sample in the pond WBC 31, and the second syringe 42 is by WBC reagent later It is reacted in the 23 injection ponds WBC 31;
Step S6: sampling needle 10 is moved to 32 position of the pond RBC, injects the diluted sample drawn from the pond WBC 31;
The pond step S7:CRP 33, the pond SAA 34, the pond WBC 31 and the pond RBC 32 carry out parallel measurement to each parameter respectively, complete The cleaning in measurement result and each channel is exported afterwards.
With no restrictions, other steps guarantee above-mentioned steps sequence to the sequencing of above-mentioned steps S2 and step S3.
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this Under the design of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/it is used in it indirectly He is included in scope of patent protection of the invention relevant technical field.

Claims (9)

1. a kind of blood test device, which is characterized in that including sampling needle, counting cell unit, syringe unit, dilution distribution Unit and negative pressure chamber;
The sampling needle is for drawing and distributing blood sample to the counting cell unit;
The counting cell unit includes the pond CRP, the pond WBC, the pond RBC, and the pond CRP is anti-as dilution, CRP reagent and blood sample Container is answered, and measures CRP parameter;The pond WBC is measured as dilution, the reaction vessel of WBC reagent and blood sample WBC quantity;The pond RBC is counted for carrying out RBC and PLT;
The dilution allocation unit is separately connected the pond CRP, the pond WBC and the pond RBC;The syringe unit will be for that will dilute Liquid is distributed via the dilution allocation unit to the pond CRP, the pond WBC and the pond RBC;The syringe unit is also used to try CRP Agent is assigned to the pond CRP, WBC reagent is assigned to the pond WBC;
The negative pressure chamber provides sample flow driving force for the pond WBC and the pond RBC.
2. blood test device as described in claim 1, which is characterized in that the blood test device do not include the pond DIFF with DIFF lane testing unit.
3. blood test device as claimed in claim 2, which is characterized in that the counting cell unit further includes the pond SAA, described The pond SAA measures SAA parameter as dilution, the reaction vessel of SAA reagent and blood sample.
4. blood test device as claimed in claim 3, which is characterized in that the pond CRP includes CRP pond body, first laser There is CRP reagent mouth, CRP waste liquid outlet is added for device and the first photodetector, the CRP pond body;The first laser device With first photodetector for CRP measurement of concetration in CRP pond body.
5. blood test device as claimed in claim 4, which is characterized in that the pond SAA includes SAA pond body, second laser There is SAA reagent mouth, SAA waste liquid outlet is added for device and the second photodetector, the SAA pond body;The second laser With second photodetector for SAA measurement of concetration in SAA pond body.
6. blood test device as claimed in claim 5, which is characterized in that the pond WBC and the pond RBC have respectively included Impedance measuring unit, each impedance measuring unit include forebay electrode, gem hole, after-bay electrode;The pond WBC and described Cell in the pond RBC passes through the gem hole one by one under the suction function of the negative pressure chamber, when cell passes through the gem hole, The forebay electrode and the after-bay electrode can generate pulse, calculate WBC quantity, RBC quantity and PLT according to number of pulses Quantity.
7. blood test device as claimed in claim 6, which is characterized in that the pond WBC further includes HGB measuring unit, institute Stating HGB measuring unit includes light emitting diode and photelectric receiver, and the light that the light emitting diode issues passes through anti-in the pond WBC It answers liquid to be transmitted to the photelectric receiver, HGB concentration is calculated according to the transmitted intensity that the photelectric receiver receives.
8. blood test device as claimed in claim 7, which is characterized in that the blood routine detection unit further includes that refrigeration is single Member, the refrigeration unit are used to control the SAA reagent and the CRP temperature of reagent at 2-8 DEG C.
9. blood test device as claimed in claim 8, which is characterized in that the blood routine detection unit further includes preheating Unit, the preheating unit carry out the SAA reagent, CRP reagent and dilution in advance before being added to each reaction tank Heating.
CN201910319409.2A 2019-04-19 2019-04-19 Blood test device Pending CN109932519A (en)

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