CN112444620A - Blood cell analyzer and counting method thereof - Google Patents
Blood cell analyzer and counting method thereof Download PDFInfo
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- CN112444620A CN112444620A CN201910815336.6A CN201910815336A CN112444620A CN 112444620 A CN112444620 A CN 112444620A CN 201910815336 A CN201910815336 A CN 201910815336A CN 112444620 A CN112444620 A CN 112444620A
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- 238000000034 method Methods 0.000 title claims abstract description 99
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 48
- 210000004027 cell Anatomy 0.000 claims abstract description 199
- 238000001514 detection method Methods 0.000 claims abstract description 148
- 238000012360 testing method Methods 0.000 claims abstract description 112
- 239000012085 test solution Substances 0.000 claims abstract description 88
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- 230000008569 process Effects 0.000 claims abstract description 32
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- LRFVTYWOQMYALW-UHFFFAOYSA-N Xanthine Natural products O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 1
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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Abstract
The application provides a blood cell analyzer and a counting method thereof, wherein the counting method comprises the following steps: carrying out impedance counting test on the original test solution to be tested in the counting cell, and detecting whether the detection hole is blocked or not in the counting test process; when the occurrence of hole blockage is detected, sucking out at least part of the original test solution from the counting cell; performing blockage removal operation on the detection hole; injecting the original test solution sucked out of the counting cell back to the counting cell; and carrying out impedance counting test again on the original test solution injected back to the counting cell. The original test solution can be obtained by initial sample preparation or diluted by a diluent. Through the scheme, the problems of resampling and sample preparation of a user can be avoided, the user experience and the test efficiency are improved, and the accuracy of a counting result can be ensured.
Description
Technical Field
The invention relates to the technical field of blood analysis, in particular to a blood cell analyzer and a counting method thereof.
Background
The impedance method blood cell analyzer has a large market share in the market of the blood cell analyzer, and the main principle is that a diluted blood sample is subjected to tests on parameters such as white blood cells, red blood cells and platelets through micropores (also called detection holes in the text), the micropores can be reduced by substances except cells mixed in the sample passing through the micropores, whether the micropores are blocked or not can be monitored by testing the pore voltage on the two sides of the micropores in real time, and when the pore voltage exceeds a set value, the sample blocking the pores is alarmed, and a relevant result of the micropore test is not given. After the instrument performs the blockage removal operation, the user performs the test again, so that the operation problem is that the current test result is invalid as long as the hole is blocked in the test process; for testing human blood such as capillary venous blood and animal samples such as rats and mice, blood needs to be taken again by a user, and the use experience of the user is influenced.
Therefore, in view of the above problems, the present application provides a new blood cell analyzer and a counting method thereof.
Disclosure of Invention
One aspect of the present application provides a counting method of a blood cell analyzer, the counting method including:
carrying out impedance counting test on the original test solution to be tested in the counting cell, and detecting whether the detection hole is blocked or not in the counting test process;
when the occurrence of hole blockage is detected, sucking out at least part of the original test solution from the counting cell;
performing blockage removal operation on the detection hole;
injecting the original test solution sucked out of the counting cell back to the counting cell;
and carrying out impedance counting test again on the original test solution injected back to the counting cell.
Illustratively, the plugging removal operation is performed on the detection hole, and comprises the following steps:
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the original test solution remained in the front cell of the counting cell,
the detection hole is backflushed to remove the blockage, so that the liquid in the rear pool of the counting pool reversely flows back to the front pool of the counting pool to flush out foreign matters which cause the blockage of the hole,
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to discharge liquid in the front cell of the counting cell;
or
Adding a first diluent to the counting cell, wherein the first diluent is used as a dielectric medium,
applying voltage on two sides of the detection hole to burn the detection hole,
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
Illustratively, the plugging removal operation is performed on the detection hole, and comprises the following steps:
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the residual original test solution in the front cell of the counting cell;
performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage;
adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium;
applying voltage to two sides of the detection hole to burn the detection hole;
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
Illustratively, the plugging removal operation is performed on the detection hole, and comprises the following steps:
adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium;
applying voltage to two sides of the detection hole to burn the detection hole;
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell;
performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage;
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
After the plugging removal operation is performed on the detection hole, before or after the original test solution sucked from the counting cell is injected back into the counting cell, the method further includes:
adding a second diluent into the counting cell to dilute the original test solution;
or
Adding a second diluent into the counting cell to dilute the original test solution;
and uniformly mixing the original test solution and the second diluent which are injected back to the counting cell.
Illustratively, the amount of the second diluent is equal to the amount of the original test solution sucked out of the counting cell.
Illustratively, the method further comprises:
obtaining a detection signal generated after the original solution is detected based on an impedance method;
determining a test result based on the detection signal, the test result including a count result;
and storing and displaying the test result on a display interface.
Illustratively, detecting whether the detection hole is blocked in the counting test process comprises the following steps:
detecting the voltage of the detection hole in real time in the counting test process;
and judging whether hole blockage occurs or not according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, the hole blockage is judged not to occur, and when the voltage of the detection hole is larger than or equal to the threshold voltage, the hole blockage is judged to occur.
Illustratively, the method further comprises:
after the blockage removing operation, continuously detecting whether the detection hole is blocked;
when the hole plugging is still detected, alarming to prompt the hole plugging; and/or
And calling a hole plugging processing flow according to the alarm to process the plugged holes.
A further aspect of the present application provides a blood cell analyzer, comprising:
the impedance method counting test system comprises a counting pool and a sensor, wherein the sensor is provided with a detection hole, and the impedance method counting test system is used for carrying out impedance method counting test on original test solution to be tested in the counting pool;
a sampling needle assembly for sucking at least part of the original test solution out of the counting cell when the occurrence of hole blockage is detected, and injecting the original test solution sucked out of the counting cell back into the counting cell after blockage removal operation;
the blockage removing device is used for performing blockage removing operation on the detection hole;
the signal processing and control device is used for: and detecting whether the detection hole is blocked or not in the counting test process, and controlling the impedance method counting test system to perform impedance method counting test on the original test solution injected back to the counting cell again.
Illustratively, the blockage removing device comprises a burning circuit and/or a backflushing blockage removing device, wherein the burning circuit is used for applying voltage to two sides of the detection hole to burn the detection hole, and the backflushing blockage removing device is used for backflushing the detection hole to remove blockage, so that liquid in the rear pool of the counting pool reversely flows back to the front pool of the counting pool to flush out foreign matters causing the blockage.
Illustratively, the signal processing and control device controls a sampling needle assembly to suck at least part of the original test solution out of the counting cell and then outputs an emptying control signal to a drainage control valve, and the drainage control valve is switched to an open state according to the emptying control signal to empty the residual original test solution in the counting cell.
Illustratively, the hemocyte analyzer further comprises a priming system for adding a first diluent to the counting cell prior to firing to serve as a dielectric for the firing circuit.
The liquid adding system is further used for adding a second diluent into the counting cell after the blockage removing operation is performed on the detection hole so as to dilute the original test solution.
The impedance counting test system is further used for detecting the voltage of the detection hole in real time during the counting test process;
the signal processing and control device is used for judging whether hole plugging occurs or not according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, the detection hole is judged not to be plugged, and when the voltage of the detection hole is larger than or equal to the threshold voltage, the detection hole is judged to be plugged.
In conclusion, by the scheme, the counting process of the hole blocking event can still obtain an accurate test result through the test of the original test solution, the problems of re-sampling and sample preparation of a user are avoided, the user experience and the test efficiency are improved, and the accuracy of the counting result can be ensured.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive labor.
FIG. 1 shows a schematic block diagram of a blood cell analyzer of one embodiment of the present application;
FIG. 2 shows a partial schematic view of a blood cell analyzer according to an embodiment of the present application;
FIG. 3 shows a partial schematic view of a blood cell analyzer of another embodiment of the present application;
FIG. 4 shows a flow chart of a counting method of the blood cell analyzer according to one embodiment of the present application;
FIG. 5 is a flowchart showing a counting method of a blood cell analyzer according to still another embodiment of the present application;
FIG. 6 shows a schematic diagram of bubble blending in an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, exemplary embodiments according to the present application will be described in detail below with reference to the accompanying drawings. It should be understood that the described embodiments are only some embodiments of the present application and not all embodiments of the present application, and that the present application is not limited by the example embodiments described herein. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the application described in the application without inventive step, shall fall within the scope of protection of the application.
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present application. It will be apparent, however, to one skilled in the art, that the present application may be practiced without one or more of these specific details. In other instances, well-known features of the art have not been described in order to avoid obscuring the present application. It is to be understood that the present application is capable of implementation in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the application to those skilled in the art.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. As used herein, the term "and/or" includes any and all combinations of the associated listed items.
In order to provide a thorough understanding of the present application, a detailed structure will be presented in the following description in order to explain the technical solutions presented in the present application. Alternative embodiments of the present application are described in detail below, however, the present application may have other implementations in addition to these detailed descriptions.
Specifically, the blood cell analyzer and the counting method thereof according to the present application will be described in detail below with reference to the accompanying drawings. The features of the following examples and embodiments may be combined with each other without conflict.
First, fig. 1 shows a schematic block diagram of a blood cell analyzer in one embodiment of the present application.
Blood cell analyzers are used for various analyses of blood components, such as counting and classifying leukocytes in blood, detecting the concentration of Hemoglobin (HGB) in erythrocytes, counting platelets, and the like.
As shown in fig. 1, the blood cell analyzer includes at least one reaction cell 12 and a sampling needle assembly 11, wherein the reaction cell 12 is used for preparing a blood sample to be analyzed into a sample solution, and the sampling needle assembly 11 is used for discharging the blood sample to be analyzed into the reaction cell 12. In further embodiments, the sampling needle assembly 11 can be implemented in other ways than a sampling needle.
In one example, the blood cell analyzer further includes a priming system 13, which may include a reagent storage device (not shown) connected to the reaction cell 12 for supplying a reagent for preparing a test solution, such as a hemolytic agent, a diluent, or the like, into the reaction cell 12. The number of the reagent storage devices is set according to the type of the reagent, for example, the reagent storage device includes a storage device for storing a hemolytic agent and a storage device for storing a diluent, wherein the storage device for storing the hemolytic agent may be further divided into one or more according to the type of the hemolytic agent.
In one example, the priming system 13 further comprises a reagent pushing component (not shown) for pushing a reagent such as a hemolytic agent, a diluent, or the like to the reaction cell 12, and the reagent pushing component may be connected to the hemolytic agent storage device and the reaction cell to push the corresponding hemolytic agent to the reaction cell 12. After the reagent is injected into the reaction cell, the blood sample, the hemolyzing agent, the diluent, and the like are mixed in the reaction cell 12 to obtain an original sample solution for the impedance method counting test
The blood cell analyzer 10 further comprises a delivery device (not shown) for delivering the original sample solution in the reaction cell 12 to the impedance method counting test system 14, in this embodiment, the delivery device comprises an injector and a delivery pipeline communicated with the injector, the original sample solution output port of the reaction cell and the inlet of the impedance method counting test system are communicated through the delivery pipeline, the number of the injectors may be multiple, and each injector performs suction and discharge actions under the control of the control device.
In one example, as shown in FIG. 1, the blood cell analyzer 10 further includes an impedance counting test system 14, and it is understood that the impedance counting test system 14 may be integrally formed with the reaction cell 12 or may be separately provided, and is not limited thereto. Impedance counting test systems are used to detect cells in an original test solution and generate a detection signal, such as a pulse signal. For example, the impedance counting test system is used for detecting white blood cells in an original test solution and outputting a pulse signal when the white blood cells pass through a detection hole in the impedance counting test system, wherein the number of the pulse signals is proportional to the number of the cells, the height of the pulse signals is proportional to the volume of the cells, and therefore the number and the volume value of blood cell particles in blood are obtained.
In one example, as shown in fig. 2, the structure of the impedance method counting test system may include a counting cell 141 and a pulse sensor, optionally, the pulse sensor may include a small-hole tube, the small-hole tube is provided with a detection hole 142, optionally, the diameter of the detection hole is less than 100 micrometers, the thickness range is 60 micrometers to 90 micrometers, for example, about 75 micrometers, the counting cell is filled with a conductive solvent (e.g., a raw test solution), and the detection hole 142 is divided into a front cell 1411 and a rear cell 1412; the front cell 1411 and the rear cell 1412 are respectively provided with a positive electrode 143 and a negative electrode 144, the positive and negative electrodes are connected with one end of a constant current source, the electrode arranged in the front cell, the conductive solvent, the electrode arranged in the rear cell and the constant current source form a series-connected closed loop (such as an analysis circuit), the rear cell 1412 of the counting cell is also provided with a liquid outlet (not shown) communicated with a negative pressure chamber, wherein, because the rear cell 1412 of the counting cell is under negative pressure, the front cell is in the front cell. When the power is switched on, the electrodes positioned at the two sides of the small-hole tube generate stable current, and under the action of negative pressure of the rear pool, diluted cell suspension (also called original test solution in the text) flows from the outer side of the small-hole tube (namely the front pool of the counting pool) to the rear pool through the detection hole, so that the resistance in the small-hole induction area is increased, instantaneous voltage change is caused to form a pulse signal, the amplitude of the pulse signal is in direct proportion to the size of the cell volume, and the pulse number is in direct proportion to the cell number, thereby obtaining the number and the volume value of blood cells in the test solution, and distinguishing different types of cells according to the volume distribution.
The counting cell and the reaction cell may be the same device or different devices, and when the counting cell and the reaction cell are the same device, the liquid adding system and the sampling needle assembly in the foregoing may be directly connected to the counting cell, and the preparation of the test solution is performed in the counting cell to obtain the original test solution, for example, the sampling needle assembly is controlled to suck a blood sample to be tested and inject the blood sample into the counting cell; and performing sample preparation operation on the blood sample injected into the counting cell to obtain the original test solution, wherein the sample preparation operation comprises injecting a sample preparation reagent into the counting cell to perform sample preparation operation to obtain the original test solution, and the sample preparation reagent comprises diluent and/or hemolytic agent and the like.
Continuing with fig. 1, the signal processing and control device 15 receives the detected pore voltage (i.e., the pulse signal) and performs shaping and signal recognition processes to form a recognition and statistical result, which may optionally be printed out directly or output to a display for viewing by an operator. The recognition and statistical results may also be stored for later review.
The diluted blood sample (namely original test solution) is subjected to the test of parameters such as white blood cells, red blood cells and platelets through the detection hole, substances except cells mixed in the sample passing through the detection hole can make the detection hole smaller. After the instrument performs the blockage removal operation, the user performs the test again, so that the operation problem is that the current test result is invalid as long as the hole is blocked in the test process; for testing human blood such as capillary venous blood and animal samples such as rats and mice, blood needs to be taken again by a user, and the use experience of the user is influenced.
Therefore, in order to solve the above technical problem, an embodiment of the present application provides a counting method of a blood cell analyzer, the counting method including: carrying out impedance counting test on the original test solution to be tested in the counting cell, and detecting whether the detection hole is blocked or not in the counting test process; when the occurrence of hole blockage is detected, sucking out at least part of the original test solution from the counting cell; performing blockage removal operation on the detection hole; injecting the original test solution sucked out of the counting cell back to the counting cell; and carrying out impedance counting test again on the original test solution injected back to the counting cell. The original test solution can be obtained by initial sample preparation or diluted by a diluent. Through the scheme, the counting process of the hole blocking event can still obtain an accurate test result through the test of the original test solution, the problems of re-sampling and sample preparation of a user are avoided, the user experience and the test efficiency are improved, and the accuracy of the counting result can be ensured.
Hereinafter, the counting method of the blood cell analyzer according to the embodiment of the present application will be explained and explained in detail with reference to the drawings.
The counting method of the blood cell analyzer in the embodiment of the application comprises the following steps:
first, as shown in fig. 4, in step S401, an impedance counting test is performed on an original test solution to be tested in a counting cell, and whether a hole is blocked in the detection hole is detected in the counting test process.
The original sample solution is prepared by the method described above, and may be any animal or human blood sample, including white blood cells, red blood cells, platelets, etc., which can be obtained by treating whole blood drawn from a human or animal with a diluent, a hemolytic agent, etc. The diluent is an isotonic solution with acid-base buffering effect, proper ionic strength and conductivity, for example, the diluent mainly contains hypoxanthine or xanthine compound or its salt as main component or other diluent capable of playing the above role, and the hemolytic agent is used for lysing erythrocytes for classifying and counting leukocytes. The hemolytic agent includes a surfactant, which may specifically include a cationic surfactant and a nonionic surfactant. The amount and concentration of the hemolytic agent may be selected reasonably according to actual sample preparation requirements, and are not specifically limited herein. The hemolytic agent may be a quaternary ammonium salt ionic surfactant as a main component, or may be any other surfactant capable of exerting the above-described effects.
In one example, the procedure of the method of performing an impedance counting test on the original test solution to be tested in the counting cell may be as follows: the sampling needle assembly sucks a certain amount of blood from the blood collection tube, injects the blood into the counting cell and uniformly mixes the blood with a certain amount of diluent to form diluted sample solution with a fixed dilution ratio, namely original sample solution, and then carries out impedance counting test in the counting cell to further obtain a counting result. The method comprises the steps of firstly diluting in a front pool of a counting pool to obtain original test solution, and then passing the front pool through a detection hole to a rear pool under the action of negative pressure. In the counting process, the original test solution flows into the rear tank from the front tank to form a stable flow field. Since a constant current source is applied between the positive electrode and the negative electrode, a stable electric field is formed as shown in fig. 2. A pulse signal is formed when the blood cells pass through the detection hole 142, as shown in fig. 2. When foreign objects are blocked in front of the detection holes, the voltage of the detection holes can be increased or abnormally fluctuated, and therefore whether the detection holes are blocked or not can be judged.
In one specific example, detecting whether the detection hole is blocked in the counting test process comprises: detecting the voltage of the detection hole in real time in the counting test process; and judging whether hole blockage occurs or not according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, the hole blockage is judged not to occur, and when the voltage of the detection hole is larger than or equal to the threshold voltage, the hole blockage is judged to occur. The threshold voltage may be reasonably set according to a priori experience, and is not specifically limited herein.
Then, as shown in fig. 4, in step S402, when the occurrence of hole blockage is detected, at least part of the original sample solution is sucked out of the counting cell.
In step S402, the sampling needle assembly may be controlled to suck out a portion of the original sample solution in the counting chamber from the counting chamber, wherein the amount of the sucked out original sample solution may be appropriately set according to actual needs, for example, about 50% of the original sample solution is sucked out from the counting chamber, and the sucked out original sample solution may be stored in the sampling needle assembly and/or a pipeline connected to the sampling needle assembly, or other sample solution storage device.
Next, as shown in fig. 4, in step S403, performing a blockage removing operation on the detection hole;
performing a blockage removing operation on the test hole, where the blockage removing operation may be any suitable blockage removing operation process known to those skilled in the art, for example, in this embodiment, performing a blockage removing operation on the test hole includes: controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the residual original test solution in the front cell of the counting cell; and (3) performing backflushing blockage removal operation on the detection hole to enable liquid in the rear pool of the counting pool to reversely flow back to the front pool of the counting pool so as to flush the foreign matters causing the blockage holes, specifically, applying positive pressure to the rear pool of the counting pool through a positive and negative pressure device to enable the liquid in the rear pool of the counting pool to reversely flow back to the front pool of the counting pool so as to flush the foreign matters causing the blockage holes, thereby removing the blockage. Optionally, the blockage removing operation may further include: after the back flushing blockage removing operation, the liquid discharge control valve of the counting cell is controlled to be switched to an open state so as to discharge the liquid in the front cell of the counting cell, and the step can be selectively performed according to actual needs, for example, when only the back flushing blockage removing operation is performed, the discharging step can be performed, and if other blockage removing operations are performed after the back flushing blockage removing operation, and the liquid in the front cell of the counting cell does not influence the subsequent blockage removing operation, the discharging step can also not be performed.
In one example, the plugging removal operation is performed on the detection hole, and the method further comprises the following steps: adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium; applying voltage to two sides of the detection hole to burn the detection hole, and under the premise that the first diluent is used as a dielectric medium, for example, in the example shown in fig. 3, by a burning circuit, namely, adding a strong current between the positive electrode 143 and the negative electrode 144, when blockage needs to be removed, the burning circuit is electrified to form local high temperature at the detection hole 142, even to boil the liquid, so as to destroy a foreign matter structure near the detection hole 142; after ignition, controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell, namely, to empty the liquid in a front cell of the counting cell, so as to facilitate the subsequent counting process. Optionally, the following steps may optionally be performed prior to adding the first diluent: and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to discharge the liquid (such as the residual original test solution) in the front cell of the counting cell, or directly adding the first diluent as a dielectric medium without performing the discharge step, wherein the discharge control valve can be reasonably selected according to actual needs.
In one example, the blockage removing operation is performed on the detection hole, and the backflushing operation and the burning operation are performed, for example, a liquid discharge control valve of the counting cell is controlled to be switched to an open state, so that the original test liquid remaining in the front cell of the counting cell is emptied; performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage; adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium; applying voltage to two sides of the detection hole to burn the detection hole; and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell. The burning operation can be performed first and then the backflushing operation can be performed as required, for example: adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium; applying voltage to two sides of the detection hole to burn the detection hole; controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell; performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage; and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
Continuing with FIG. 4, in step S404, the original sample solution sucked out of the counting cell is injected back into the counting cell.
In one example, after the plugging removal operation is performed on the detection hole, before or after the original test solution sucked from the counting cell is injected back into the counting cell, optionally: the second diluent is added into the counting cell to dilute the original sample solution, that is, dilute the original sample solution injected back into the counting cell, because only a part of the original sample solution is sucked out in the foregoing step, when the part of the original sample solution is injected back into the front cell of the counting cell again, the amount of the part of the original sample solution is insufficient, for example, the liquid level of the part of the original sample solution is lower than the detection hole, so that the counting test cannot be performed, and therefore the part of the original sample solution injected back into the counting cell needs to be diluted. The amount of the second diluent can be reasonably selected according to the requirement, and preferably, the amount of the second diluent is equal to the amount of the original test solution sucked out from the counting cell.
It should be noted that the second diluent may be the same as the first diluent or may be a different diluent, and is not particularly limited herein.
In one example, it is also possible to selectively: and uniformly mixing the original test solution injected back to the counting cell with the second diluent, so that blood cells and the like in the diluted original test solution are uniformly distributed.
As shown in fig. 4, in step S405, the impedance counting test is performed again on the original sample solution injected back to the counting cell, where the original sample solution may be an original sample prepared to obtain an original sample solution, or an original sample solution diluted by a diluent. By the scheme, the counting process of the hole blocking event can still obtain an accurate test result through the test of the original test solution, the problems of re-sampling and sample preparation of a user are avoided, and the user experience and the test efficiency are improved. The method of impedance counting test can refer to the description above, and is not described herein.
Fig. 5 shows a specific embodiment of sucking out a part of original test solution, performing a blockage removal operation, and finally performing a counting test, and the specific flow is as shown in fig. 5:
firstly, obtaining a diluted blood sample, namely an original test solution, through a sample preparation operation, wherein the specific operation can be referred to the foregoing description and is not described herein any more; then, in the counting process, under the action of negative pressure, the diluted blood sample (namely, the original test solution) flows from the front pool to the rear pool through the detection hole to form a stable flow field. Since a constant current source is applied between the positive electrode and the negative electrode, a stable electric field is formed. When blood cells pass through the detection hole, a pulse signal is formed, and when foreign objects are blocked in front of the detection hole, the voltage of the detection hole is increased or abnormally fluctuated, so that whether the detection hole is blocked or not can be judged; then, in the counting process, detecting the voltage of the detection hole in real time, and judging whether hole plugging occurs according to whether the voltage of the detection hole is smaller than a threshold voltage, wherein when the voltage of the detection hole is smaller than the threshold voltage, judging that the hole plugging does not occur, and executing the following steps:
acquiring a detection signal, such as a pulse signal, generated after the original solution is detected based on an impedance method; determining a test result based on the detection signal, wherein the test result comprises a counting result, and specifically, calculating the detection signal to determine the test result; and storing and displaying the test result on a display interface.
Continuing as shown in fig. 5, when the voltage of the detection hole is not less than the threshold voltage, that is, is greater than or equal to the threshold voltage, it is determined that the hole blockage occurs, and the following operation steps are performed:
firstly, 50% of a blood sample (namely original test solution) diluted in a counting cell is sucked into a pipeline connected with a sampling needle through the sampling needle;
then, emptying the counting cell, for example, controlling a liquid discharge control valve of the counting cell to be switched to an open state, so as to empty the original test solution remaining in the front cell of the counting cell;
then, carrying out backflushing blockage removal operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush out foreign matters which cause hole blockage;
then, adding a diluent into the counting cell; applying voltage to two sides of the detection hole to burn the detection hole;
then, after ignition, controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell;
then, adding diluent which is equal to the original test solution stored in the sampling needle assembly and the corresponding pipeline into the counting cell;
then, uniformly mixing the original test solution and the diluent which are injected back to the counting cell;
continuing as shown in fig. 5, after the above operation, performing impedance counting test again on the original test solution (in this case, the diluted original test solution) in the counting cell, that is, in the counting process, under the action of the negative pressure, the diluted original test solution flows from the front cell to the rear cell through the detection hole, so as to form a stable flow field. And after the blockage removing operation, continuously detecting whether the detection hole is blocked, namely continuously detecting the voltage of the detection hole in real time in the counting process, and when the voltage of the detection hole is smaller than the threshold voltage, judging that the hole is not blocked, and executing the following steps: acquiring a detection signal, such as a pulse signal, generated after the original solution is detected based on an impedance method; determining a test result based on the detection signal, wherein the test result comprises a counting result, and specifically, calculating the detection signal to determine the test result; and storing and displaying the test result on a display interface.
Continuing with fig. 5, when the voltage of the detection hole is not less than the threshold voltage, that is, is greater than or equal to the threshold voltage, it is determined that the hole is still blocked, and obviously the blocking removal operation does not play a role in removing the blocking, the following steps are performed: alarming to prompt the plugging of the hole, namely reporting the plugging fault; the method comprises the steps of calling a plugging processing flow according to the alarm to process a plugged hole, wherein the plugging processing flow is also used for starting fault processing, and the plugging removal operation can be different from the plugging removal operation described in the previous step. It will be appreciated that both the plugging hole failure and the start failure processing may be performed alternatively or simultaneously.
Based on the counting method of the blood cell analyzer, the present embodiment also provides a blood cell analyzer, which can be used for executing the counting method in the foregoing embodiment, and the description of some structures of the blood cell analyzer can also refer to the description in the foregoing, and only some features in the blood cell analyzer are explained and illustrated herein.
Continuing with fig. 1, the blood cell analyzer includes an impedance counting test system 14, which, as shown in fig. 2 and 3, includes a counting cell 141 and a pulse sensor, wherein the pulse sensor is provided with a detection hole 142, and the impedance counting test system 14 is used for performing an impedance counting test on the original test solution to be tested in the counting cell.
In one example, the blood cell analyzer 100 further includes a signal processing and control device 15 for detecting whether the detection hole is blocked during the counting test, and controlling the impedance counting test system to perform the impedance counting test again on the original test solution injected back into the counting chamber. The signal processing and control device 15 may include an analysis circuit as shown in fig. 2, and specifically, the signal processing and control device may receive a detection signal, such as a pulse signal, output by the impedance method counting test system 14, and perform processing, operation, and the like on the pulse signal, so as to obtain a corresponding counting result.
The blood cell analyzer 100 also includes a storage device (not shown) that may include one or more computer program products that may include various forms of computer-readable storage media, such as volatile memory and/or non-volatile memory. The volatile memory may include, for example, Random Access Memory (RAM), cache memory (cache), and/or the like. The non-volatile memory may include, for example, Read Only Memory (ROM), hard disk, flash memory, etc., which may be used to store test results, etc.
In a specific example, the signal processing and control device 15 is further configured to: when the proportion of the time that the voltage of the detection hole is greater than or equal to the threshold voltage in the impedance counting test exceeds the time threshold in the total time of the impedance counting test, the counting result is in accordance with the condition, the counting result is recorded, for example, the counting result is transmitted to an output device, for example, the output device can output various information (such as images or sound) to the outside (such as a user), and can comprise one or more of a display, a loudspeaker and the like.
In one specific example, the blood cell analyzer further comprises a blocking removal device for: when the hole blockage is detected to occur, carrying out blockage removal operation on the detection hole; the signal processing and control device is used for: and after the blockage removal operation, controlling the impedance method counting test system to perform impedance method counting test on the original test solution in the counting cell again.
In one example, as shown in fig. 3, the blockage removing device includes a burning circuit, which is configured to apply a voltage to two sides of the detection hole in the original test solution environment, so as to burn the detection hole. Or, the burning circuit can be used for applying voltage to two sides of the detection hole under the environment of the diluent so as to burn the detection hole. Wherein, the burning circuit is connected between the positive electrode 143 and the negative electrode 144, and is electrically connected with the power supply during operation.
In one example, the blockage removing device may further include a backflushing blockage removing device (not shown) for performing a backflushing blockage removing operation on the detection hole to reversely flow the liquid in the rear pool of the counting pool back to the front pool of the counting pool so as to flush out foreign matters causing the blockage hole. The back-flushing blockage removing device can comprise a positive and negative pressure device to form positive pressure in the rear pool of the counting pool so as to enable liquid in the rear pool of the counting pool to reversely flow back to the front pool of the counting pool.
In an example, the blockage removing device 16 further includes a blending device, as shown in fig. 6, configured to perform blending operation on the original test solution in the counting cell after the ignition, so as to reduce an influence of bubbles generated by the ignition on the impedance counting test result.
In one example, the blood cell analyzer further comprises a blocking device 16 and a sampling needle assembly 11, when the occurrence of hole blocking is detected, the sampling needle assembly sucks at least part of the original test solution out of the counting cell; the blockage removing device 16 is used for performing blockage removing operation on the detection hole; the sampling needle assembly 11 is further used for injecting the original test solution sucked out from the counting cell back to the counting cell after the blockage removing operation; the signal processing and control device 15 is further configured to control the impedance counting test system 14 to perform the impedance counting test on the original test solution injected back to the counting cell again.
In one example, the counting cell further comprises a liquid inlet pipe coupled to the liquid feeding system, a liquid outlet pipe coupled to the liquid storage system (not shown), and a liquid discharge pipe provided with a liquid discharge control valve (not shown) switchable between a closed state and an open state according to a control signal.
In one example, the signal processing and control device 15 controls the sampling needle assembly to suck at least part of the original test solution out of the counting cell and then outputs an emptying control signal to the liquid discharge control valve, the liquid discharge control valve is switched to an open state according to the emptying control signal to empty the remaining original test solution in the counting cell, or after burning, the signal processing and control device 15 controls the liquid discharge control valve of the counting cell to be switched to an open state to empty the liquid in the counting cell.
In one example, the blood cell analyzer further includes a priming system 13 for adding a first diluent to the counting cell prior to firing to serve as a dielectric for the firing circuit. The method can also be used for adding a second diluent into the counting cell after the blockage removing operation is carried out on the detection hole so as to dilute the original test solution.
In one example, the impedance counting test system 14 is also used to detect the voltage of the detection well in real time during the counting test; the signal processing and control device 15 is configured to determine whether a hole plugging occurs according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, it is determined that the hole plugging does not occur, and when the voltage of the detection hole is greater than or equal to the threshold voltage, it is determined that the hole plugging occurs.
In one example, the blood cell analyzer further includes an alarm device (not shown) for giving an alarm to prompt clogging when clogging is still detected after the clogging removal operation. And the user can call the hole plugging processing flow according to the alarm to process the plugged holes.
In summary, according to the blood cell analyzer and the counting method thereof in the embodiment of the application, when a hole blockage is detected, the test is suspended, the original test solution is partially sucked out, then the blockage removing operation is performed, and finally the sucked original test solution is injected back to the counting cell for retesting.
Although the example embodiments have been described herein with reference to the accompanying drawings, it is to be understood that the above-described example embodiments are merely illustrative and are not intended to limit the scope of the present application thereto. Various changes and modifications may be effected therein by one of ordinary skill in the pertinent art without departing from the scope or spirit of the present application. All such changes and modifications are intended to be included within the scope of the present application as claimed in the appended claims.
Those of ordinary skill in the art will appreciate that the various illustrative elements and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware or combinations of computer software and electronic hardware. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the implementation. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present application.
In the several embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other ways. For example, the above-described device embodiments are merely illustrative, and for example, the division of the units is only one logical functional division, and other divisions may be realized in practice, for example, a plurality of units or components may be combined or integrated into another device, or some features may be omitted, or not executed.
In the description provided herein, numerous specific details are set forth. However, it is understood that embodiments of the application may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.
Similarly, it should be appreciated that in the description of exemplary embodiments of the present application, various features of the present application are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the application and aiding in the understanding of one or more of the various inventive aspects. However, the method of the present application should not be construed to reflect the intent: this application is intended to cover such departures from the present disclosure as come within known or customary practice in the art to which this invention pertains. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this application.
It will be understood by those skilled in the art that all of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and all of the processes or elements of any method or apparatus so disclosed, may be combined in any combination, except combinations where such features are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, abstract and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise.
Furthermore, those skilled in the art will appreciate that while some embodiments described herein include some features included in other embodiments, rather than other features, combinations of features of different embodiments are meant to be within the scope of the application and form different embodiments. For example, in the claims, any of the claimed embodiments may be used in any combination.
The various component embodiments of the present application may be implemented in hardware, or in software modules running on one or more processors, or in a combination thereof. Those skilled in the art will appreciate that a microprocessor or Digital Signal Processor (DSP) may be used in practice to implement some or all of the functionality of some of the modules according to embodiments of the present application. The present application may also be embodied as apparatus programs (e.g., computer programs and computer program products) for performing a portion or all of the methods described herein. Such programs implementing the present application may be stored on a computer readable medium or may be in the form of one or more signals. Such a signal may be downloaded from an internet website or provided on a carrier signal or in any other form.
It should be noted that the above-mentioned embodiments illustrate rather than limit the application, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The application may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The usage of the words first, second and third, etcetera do not indicate any ordering. These words may be interpreted as names.
Claims (15)
1. A counting method of a blood cell analyzer, comprising:
carrying out impedance counting test on the original test solution to be tested in the counting cell, and detecting whether the detection hole is blocked or not in the counting test process;
when the occurrence of hole blockage is detected, sucking out at least part of the original test solution from the counting cell;
performing blockage removal operation on the detection hole;
injecting the original test solution sucked out of the counting cell back to the counting cell;
and carrying out impedance counting test again on the original test solution injected back to the counting cell.
2. The counting method of claim 1, wherein performing a plug removal operation on the test well comprises:
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the original test solution remained in the front cell of the counting cell,
the detection hole is backflushed to remove the blockage, so that the liquid in the rear pool of the counting pool reversely flows back to the front pool of the counting pool to flush out foreign matters which cause the blockage of the hole,
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to discharge liquid in the front cell of the counting cell;
or
Adding a first diluent to the counting cell, wherein the first diluent is used as a dielectric medium,
applying voltage on two sides of the detection hole to burn the detection hole,
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
3. The counting method of claim 1, wherein performing a plug removal operation on the test well comprises:
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the residual original test solution in the front cell of the counting cell;
performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage;
adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium;
applying voltage to two sides of the detection hole to burn the detection hole;
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
4. The counting method of claim 1, wherein performing a plug removal operation on the test well comprises:
adding a first diluent into the counting cell, wherein the first diluent is used as a dielectric medium;
applying voltage to two sides of the detection hole to burn the detection hole;
controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell;
performing backflushing and blockage removing operation on the detection hole to enable liquid in a rear pool of the counting pool to reversely flow back to a front pool of the counting pool so as to flush foreign matters causing hole blockage;
and controlling a liquid discharge control valve of the counting cell to be switched to an open state so as to empty the liquid in the counting cell.
5. The counting method of claim 1, wherein after the detecting hole is removed, the original sample solution sucked from the counting chamber is injected back to the counting chamber, and before or after the original sample solution is injected back to the counting chamber, further comprising:
adding a second diluent into the counting cell to dilute the original test solution;
or
Adding a second diluent into the counting cell to dilute the original test solution;
and uniformly mixing the original test solution and the second diluent which are injected back to the counting cell.
6. The counting method of claim 5, wherein the amount of the second diluent is equal to the amount of the original sample solution sucked out of the counting chamber.
7. The counting method of claim 1, further comprising:
obtaining a detection signal generated after the original solution is detected based on an impedance method;
determining a test result based on the detection signal, the test result including a count result;
and storing and displaying the test result on a display interface.
8. The counting method of any one of claims 1 to 7, wherein detecting whether the detection hole is blocked during the counting test comprises:
detecting the voltage of the detection hole in real time in the counting test process;
and judging whether hole blockage occurs or not according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, the hole blockage is judged not to occur, and when the voltage of the detection hole is larger than or equal to the threshold voltage, the hole blockage is judged to occur.
9. The counting method of claim 1, further comprising:
after the blockage removing operation, continuously detecting whether the detection hole is blocked;
when the hole plugging is still detected, alarming to prompt the hole plugging; and/or
And calling a hole plugging processing flow according to the alarm to process the plugged holes.
10. A blood cell analyzer, comprising:
the impedance method counting test system comprises a counting pool and a sensor, wherein the sensor is provided with a detection hole, and the impedance method counting test system is used for carrying out impedance method counting test on original test solution to be tested in the counting pool;
a sampling needle assembly for sucking at least part of the original test solution out of the counting cell when the occurrence of hole blockage is detected, and injecting the original test solution sucked out of the counting cell back into the counting cell after blockage removal operation;
the blockage removing device is used for performing blockage removing operation on the detection hole;
the signal processing and control device is used for: and detecting whether the detection hole is blocked or not in the counting test process, and controlling the impedance method counting test system to perform impedance method counting test on the original test solution injected back to the counting cell again.
11. The blood cell analyzer of claim 10, wherein the blockage removing device comprises a burning circuit and/or a backflushing blockage removing device, the burning circuit is used for applying voltage to two sides of the detection hole to burn the detection hole, and the backflushing blockage removing device is used for backflushing the detection hole to enable liquid in the rear pool of the counting pool to reversely flow back to the front pool of the counting pool so as to flush out foreign matters causing the blockage hole.
12. The blood cell analyzer of claim 10, wherein the signal processing and control device controls the sampling needle assembly to output an evacuation control signal to the drain control valve after at least a portion of the original sample solution is aspirated from the counting chamber, and the drain control valve is switched to an open state according to the evacuation control signal to evacuate the remaining original sample solution from the counting chamber.
13. The blood cell analyzer of claim 12, further comprising a priming system for adding a first diluent to said counting cell prior to firing to serve as a dielectric for said firing circuit.
14. The blood cell analyzer of claim 13, wherein the priming system is further configured to add a second diluent to the cuvette to dilute the primary sample after the detection well is cleared.
15. The blood cell analyzer of any one of claims 10 to 14,
the impedance method counting test system is also used for detecting the voltage of the detection hole in real time in the counting test process;
the signal processing and control device is used for judging whether hole plugging occurs or not according to the voltage of the detection hole, wherein when the voltage of the detection hole is smaller than a threshold voltage, the detection hole is judged not to be plugged, and when the voltage of the detection hole is larger than or equal to the threshold voltage, the detection hole is judged to be plugged.
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