CN110871202A - Sampling needle cleaning method and blood cell analyzer - Google Patents
Sampling needle cleaning method and blood cell analyzer Download PDFInfo
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- CN110871202A CN110871202A CN201811012023.9A CN201811012023A CN110871202A CN 110871202 A CN110871202 A CN 110871202A CN 201811012023 A CN201811012023 A CN 201811012023A CN 110871202 A CN110871202 A CN 110871202A
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- 238000005070 sampling Methods 0.000 title claims abstract description 167
- 238000000034 method Methods 0.000 title claims abstract description 66
- 238000004140 cleaning Methods 0.000 title claims abstract description 59
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 142
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 77
- 210000000265 leukocyte Anatomy 0.000 claims description 46
- 210000003743 erythrocyte Anatomy 0.000 claims description 42
- 238000005259 measurement Methods 0.000 claims description 41
- 210000004027 cell Anatomy 0.000 claims description 34
- 102000001554 Hemoglobins Human genes 0.000 claims description 23
- 108010054147 Hemoglobins Proteins 0.000 claims description 23
- 239000003085 diluting agent Substances 0.000 claims description 22
- 210000001772 blood platelet Anatomy 0.000 claims description 21
- 238000005406 washing Methods 0.000 claims description 9
- 210000003924 normoblast Anatomy 0.000 claims 2
- 239000000523 sample Substances 0.000 description 51
- 210000004369 blood Anatomy 0.000 description 11
- 239000008280 blood Substances 0.000 description 11
- 238000010586 diagram Methods 0.000 description 8
- 230000036647 reaction Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000002699 waste material Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000004820 blood count Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000002949 hemolytic effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/023—Cleaning the external surface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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Abstract
The application discloses sampling needle cleaning method and blood cell analyzer, and the sampling needle cleaning method comprises the following steps: in the working process of a first measuring system connected with a first cavity, a driving device drives a sampling needle to move above the first cavity and suck liquid in the first cavity, wherein the liquid is liquid containing hemolytic agent components; the driving means drives the sampling needle to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle. By the mode, the sampling needle can be cleaned, and the cost is low.
Description
Technical Field
The application relates to the technical field of medical treatment, in particular to a sampling needle cleaning method and a blood cell analyzer.
Background
The hematology analyzers currently on the market, whether for counting white blood cells, red blood cells and platelets, all need to use a sampling needle to suck a whole blood sample, and add the whole blood sample to each cavity through the sampling needle for counting, wherein the counting sample liquid of a red blood cell/platelet counting cell generally sucks diluted sample from a white blood cell/hemoglobin reaction cell.
The inventor of the application finds that before secondary sample suction, if the inner wall of the sampling needle is not cleaned completely, the sampling needle can carry red blood cells and platelets to enter a red blood cell/platelet counting pool, so that the counting of the red blood cells and the platelets is higher. Or after the blood separation is finished, if the inner wall of the sampling needle is not cleaned, the blood cells, lipid or protein and other residues carried on the sampling needle can influence the next measurement precision. When the inner wall of the sampling needle is cleaned by the existing blood cell analyzer, the inner wall is generally cleaned by using diluent or external cleaning liquid, and the problems of unclean cleaning or higher cost and the like exist.
Disclosure of Invention
The technical problem that this application mainly solved provides a sampling needle cleaning method, blood cell analysis appearance, can wash sampling needle and the cost is lower.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a sampling needle cleaning method for a blood cell analyzer, the method comprising: in the working process of a first measuring system connected with a first cavity, a driving device drives a sampling needle to move above the first cavity and suck liquid in the first cavity, wherein the liquid is liquid containing hemolytic agent components; the driving means drives the sampling needle to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle.
In order to solve the above technical problem, another technical solution adopted by the present application is: provided is a blood cell analyzer, including: a sampling needle for aspirating a sample; a plurality of chambers including a first chamber for containing a liquid containing a hemolytic agent component; the first measuring system is connected with the first cavity and used for detecting liquid contained in the first cavity; and the driving device is connected with one end of the sampling needle and used for driving the sampling needle to absorb the liquid containing hemolytic agent components in the first cavity in the working process of the first measuring system so as to clean the sampling needle.
The beneficial effect of this application is: different from the situation of the prior art, the sampling needle cleaning method provided by the application comprises the following steps: in the working process of a first measuring system connected with the first cavity, the driving device drives the sampling needle to move to the position above the first cavity containing liquid of hemolytic agent components and suck and spit the liquid in the first cavity. Compared with the traditional method of cleaning by using diluent, the liquid containing hemolytic agent components can clean blood cells, lipid or protein and other residues on the inner wall of the sampling needle, the cleaning effect is good, and the problem that counting of red blood cells, platelets and the like is abnormally high due to the fact that the diluent is not cleaned completely can be avoided. Compare with current cleaning solution washing that utilizes, commonly used hemolytic agent washs in this application utilizes the blood cell analysis appearance, need not professional cleaning solution, and the washing cost is lower.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic diagram of the structure of one embodiment of a blood cell analyzer according to the present application;
FIG. 2 is a schematic diagram of the structure of one embodiment of the sampling needle of FIG. 1;
FIG. 3 is a schematic structural view of an embodiment of the cleaning apparatus of FIG. 1;
FIG. 4 is a schematic flow chart diagram illustrating one embodiment of a method for cleaning a sampling needle according to the present application;
FIG. 5 is a schematic flow chart diagram of another embodiment of the present application of a method for cleaning a sampling needle;
FIG. 6 is a schematic flow chart diagram of another embodiment of the present application of a method for cleaning a sampling needle;
FIG. 7 is a schematic flow chart of another embodiment of the method for cleaning a sampling needle of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a blood cell analyzer according to the present application. The blood cell analyzer is one of the most commonly used screening instruments for clinical examination at home and abroad at present, and has the functions of: a complete blood cell count function (including red blood cell, white blood cell, and platelet counts and their associated computational parameters), a white blood cell sorting function (including four sorting white blood cells, etc.), a nucleated red blood cell count, a hematopoietic stem cell count, and the like. The blood cell analyzer provided by the application comprises a sampling needle 1, a plurality of cavities 3, a first measuring system 5 and a driving device 7.
In particular, the sampling needle 1 is used to aspirate a sample. In one application scenario, the blood cell analyzer further comprises a sample tube 2, and the sampling needle 1 draws a sample, which may be a whole blood sample, from the sample tube 2. In another application scenario, the sampling needle 1 may also draw a sample from one of the plurality of cavities 3, which may be a diluted blood sample. In another application scenario, please refer to fig. 2, fig. 2 is a schematic structural diagram of an embodiment of the sampling needle in fig. 1. The sampling needle 1 is of a hollow structure and comprises a needle head 10, a needle tail 12 and a liquid storage cavity 14 between the needle head 10 and the needle tail 12, wherein samples or reagents in the cavities 3 enter the liquid storage cavity 14 of the sampling needle 1 through the needle head 10, and the samples or reagents in the liquid storage cavity 14 of the sampling needle 1 flow out to the cavities 3 or the waste liquid cavity 8 through the needle head 10; the needle tail 12 part can inject related reagents (such as diluent and the like), samples and the like into the liquid storage cavity 14 of the sampling needle 1 according to needs through an external pipeline; of course, the needle tail 12 can also be used in connection with the drive 7. Generally, after the sampling needle 1 sucks a sample, the inner and outer walls of the sampling needle 1 need to be cleaned to reduce the influence thereof on the next red blood cell or platelet count.
The plurality of cavities 3 includes a first cavity 30, the first cavity 30 being for containing a liquid containing a hemolytic agent component; hemolytic agents are commonly used reagents in existing blood cell analyzers, and are mainly used in cell-related measurements. For example, in the case of white blood cell counting, in order to avoid interference of red blood cells with white blood cell detection, a diluted blood sample needs to be hemolyzed by a hemolytic agent to destroy the red blood cells, fragments of the red blood cells are far smaller than the white blood cells, and the number of the white blood cells can be counted in a special white blood cell micropore. In addition, after the blood is treated by the hemolytic agent, the red blood cells release hemoglobin while being destroyed, and the hemoglobin reacts with the hemolytic agent to form a hemoglobin derivative which enters a hemoglobin test system, so that the concentration of the hemoglobin can be measured. Of course, the hemolytic agent is also used in other detection items, such as nucleated red blood cell count, white blood cell classification, and the like. Of course, the hemolytic agent may also have other effects, for example, it may make the cell membrane of the white blood cells more permeable, make the laser more permeable, facilitate the contents of the respective cells, etc.
In an application scenario, the first cavity 30 includes at least one of a leukocyte/hemoglobin reaction cell 30a, a leukocyte four-classification reaction cell 30b, and a nucleated red blood cell reaction cell 30c, and the liquid containing a hemolytic agent component may be a whole blood sample or a liquid obtained by diluting the whole blood sample with a diluent and reacting the hemolytic agent. Wherein, the hemolytic agent is a hemolytic agent commonly used in the existing blood cell analyzer. The diluent is a diluent commonly used in the existing hematology analyzers.
In another application scenario, with continued reference to fig. 1, the blood cell analyzer provided herein further includes a plurality of reagent bottles 4 for respectively containing the diluent and the plurality of hemolytic agents, wherein the diluent and the hemolytic agents contained in the plurality of reagent bottles 4 can enter each chamber 3 along a pipeline under the action of an automatic distribution system.
The first measuring system 5 is connected to the first chamber 30, and is configured to detect the liquid contained in the first chamber 30. In one application scenario, the first measurement system 5 includes a leukocyte/hemoglobin measurement system 5a, a leukocyte four-classification measurement system 5b, and a nucleated red blood cell measurement system 5c corresponding to the first cavity 30. The first measurement system 5 is a measurement system used in the prior art, and will not be described in detail herein. In one application scenario, the leukocyte four-classification measurement system 5b and the nucleated red blood cell measurement system 5c may be the same measurement system.
The driving device 7 is connected with one end of the sampling needle 1, and is used for driving the sampling needle 1 to suck the liquid containing the hemolytic agent component in the first cavity 30 in the working process of the first measurement system 5 so as to clean the sampling needle 1. The drive means 7 may be drive means 7 as used in the prior art and will not be described in detail here. In an application scenario, the driving device 7 may include a driving motor and an injector, the needle tail 12 of the sampling needle 1 is connected with one end of the injector through a pipeline, the driving motor drives the sampling needle 1 to move under the action of a control command, and the injector connected with the sampling needle 1 performs a suction and discharge action, so as to complete the operation of sucking and spitting a sample or a liquid containing a hemolytic agent component by the sampling needle 1.
In another embodiment, with continued reference to fig. 1, the hematology analyzer further includes a cleaning device 6, and the cleaning device 6 includes a swab 60, a fluid inlet conduit 62, and a fluid outlet conduit 64 for further cleaning the inner and outer walls of the sampling needle 1. Referring to fig. 3, fig. 3 is a schematic structural diagram of an embodiment of the cleaning apparatus in fig. 1. The swab 60 comprises a body 600 provided with a cavity a, and a liquid inlet 602 and a liquid outlet 604 which are positioned on a side wall (not labeled) of the body 600 and are communicated with the cavity a, wherein a liquid inlet pipeline 62 is connected with the liquid inlet 602 so that diluent enters the cavity a through the liquid inlet pipeline 62; the outlet line 64 is connected to the outlet 604 for allowing the liquid in the chamber a to flow out. One end of the sampling needle 1 (for example, the needle tail 12 of the sampling needle 1 shown in fig. 2) is connected with the driving device 7, the other end of the sampling needle 1 (for example, the needle head 10 of the sampling needle 1 shown in fig. 2) penetrates through the cavity a of the swab 60, and the sampling needle 1 moves up and down along the cavity a of the swab 60 under the driving of the driving device 7.
In one application scenario, with continued reference to fig. 3, the liquid outlet 604 of the swab 60 is located above the liquid inlet 602, and the liquid containing the hemolytic agent component sucked by the sampling needle 1 can be discharged through the swab 60. Specifically, the driving device 7 drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 of the swab 60 until the needle 10 of the sampling needle 1 is close to the liquid outlet 604, preferably, the driving device 7 drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 of the swab 60 until the needle 10 of the sampling needle 1 is flush with the liquid outlet 604, the driving device 7 makes the liquid containing hemolytic agent component in the sampling needle 1 flow out, the liquid containing hemolytic agent component flows into the liquid outlet pipeline 64 to the waste liquid cavity 8 through the liquid outlet 604, and the waste liquid cavity 8 may be located inside the blood cell analyzer or outside the blood cell analyzer, which is not limited in this application.
In yet another application scenario, continuing to refer to fig. 3, the swab 60 may be used to further clean the inner and outer walls of the sampling needle 1. Drive arrangement 7 drive sampling needle 1's syringe needle 10 moves to liquid outlet 604 direction, and during sampling needle 1's syringe needle 10 got into cavity A, the outer wall of sampling needle 1 was sprayed to behind the diluent passed through inlet conduit 62 to inlet 602, and then washs sampling needle 1's outer wall, and the liquid that has washd flows into outlet conduit 64 to waste liquid chamber 8 behind liquid outlet 604. Drive arrangement 7 further drives the needle head 10 of sampling needle 1 and moves to the liquid outlet 604 of cavity A until needle head 10 of sampling needle 1 is close to liquid outlet 604, and preferably, drive arrangement 7 further drives needle head 10 of sampling needle 1 and moves to the liquid outlet 604 of cavity A until needle head 10 of sampling needle 1 and liquid outlet 604 flush, and the diluent flows out from needle tail 12 to stock solution chamber 14 through the external pipeline, and drive arrangement 7 makes the diluent in sampling needle 1 flow out to wash the inner wall of sampling needle 1, and the liquid that has washed flows into liquid outlet pipe 64 to waste liquid chamber 8 behind liquid outlet 604.
In yet another embodiment, with continued reference to fig. 1, chamber 3 further includes a second chamber 32, and second chamber 32 includes a red blood cell/platelet count cell 32 a. The hematology analyzer further comprises a second measurement system 9 connected to the second chamber 32; wherein the second measurement system 9 comprises a red blood cell/platelet measurement system 9 a. In one application scenario, the second measurement system 9 may be the same as the red blood cell/platelet count cell, i.e. the red blood cell/platelet count cell is measured directly.
In another embodiment, the blood cell analyzer further comprises an automatic control system 11, the automatic control system 11 is connected to the driving device 7, the first measurement system 5, and the second measurement system 9, and the automatic control system 11 may be electrically connected to the driving device 7, the first measurement system 5, and the second measurement system 9, or may be electrically connected to each other; during the operation of the first measuring system 5, the automatic control system 11 controls the driving device 7 to drive the sampling needle 1 to suck up the liquid of the hemolytic agent component contained in the first cavity 30, so as to clean the sampling needle 1.
Referring to fig. 4, fig. 4 is a schematic flow chart of an embodiment of a method for cleaning a sampling needle according to the present application, the method is applied to a blood cell analyzer in any of the above embodiments, and it should be noted that specific structures of components involved in the following method processes may be referred to in the description of the above embodiments, and are not repeated herein. The sampling needle cleaning method provided by the application comprises the following steps:
s101: during the operation of the first measuring system 5 connected to the first cavity 30, the driving device 7 drives the sampling needle 1 to move above the first cavity 30 and suck the liquid in the first cavity 30, wherein the liquid is the liquid containing the hemolytic agent component.
Specifically, in an application scenario, the first cavity 30 includes at least one of a leukocyte/hemoglobin reaction cell 30a, a leukocyte four-classification reaction cell 30b, and a nucleated red blood cell reaction cell 30c, which are commonly found in a blood analyzer, and the liquid containing a hemolytic agent component is a liquid after a sample reacts with a hemolytic agent. The first measurement system 5 comprises a leukocyte/hemoglobin measurement system 5a and/or a leukocyte four-class measurement system 5b and/or a nucleated red blood cell measurement system 5c corresponding to the first cavity 30.
S102: the driving means 7 drives the sampling needle 1 to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle 1.
Specifically, in an application scenario, the blood cell analyzer includes a cleaning device 6, the cleaning device 6 includes a swab 60, an inlet conduit 62, and an outlet conduit 64, and the step S102 specifically includes: the driving device 7 drives the sampling needle 1 to discharge the suctioned hemolyzing agent component-containing liquid through the swab 60 to the liquid discharge pipe 64 connected to the swab 60.
The method for cleaning the sampling needle can be applied to any occasions needing to clean the sampling needle, such as before secondary sample suction and after sample separation.
Referring to fig. 5, fig. 5 is a schematic flow chart of another embodiment of the method for cleaning a sampling needle according to the present application, the method is applied to the blood cell analyzer in any of the above embodiments, and it should be noted that specific structures of components involved in the following method processes may be referred to in the description of the above embodiments, and are not repeated herein. The sampling needle cleaning method provided by the application comprises the following steps:
s201: the driving device 7 drives the sampling needle 1 to suck the first sample stored in the sample tube 2 from the initial position to the sample tube 2.
Specifically, the initial position may be set in advance, and may be located at a position closer to the sample tube 2. The first sample stored in the sample tube 2 may be a whole blood sample or other sample to be tested. In one application scenario, in step S201, the driving device 7 may drive the needle 10 of the sampling needle 1 to penetrate through and protrude from the swab 60, so that the needle 10 of the sampling needle 1 may suck the first sample from the sample tube 2.
S202: cleaning the outer wall of the sampling needle 1 by using a cleaning device 6;
specifically, in an application scenario, the step S202 includes: the driving device 7 drives the needle head 10 of the sampling needle 1 to move up and down along the swab 60, the diluent is sprayed to the outer wall of the sampling needle 1 after passing through the liquid inlet pipe 62 to the liquid inlet 602, so as to clean the outer wall of the sampling needle 1, and the cleaned liquid flows into the liquid outlet pipe 64 to the waste liquid cavity 8 after passing through the liquid outlet 604.
S203: the driving means 7 drives the sampling needle 1 to add the first sample into the plurality of cavities 3.
Specifically, in an application scenario, the plurality of cavities 3 includes a first cavity 30 and a second cavity 32, the first cavity 30 includes a leukocyte/hemoglobin reaction cell 30a, a leukocyte four-classification reaction cell 30b, a nucleated red blood cell reaction cell 30c, and the like, and the second cavity 32 includes a red blood cell/platelet count cell 32 a. In one embodiment, before or after the step S203, the method provided by the present application further includes: a certain proportion of diluent is added to the plurality of cavities 3, and a certain proportion of hemolytic agent is added to the first cavity.
S204: during operation of the first measuring system 5 connected to the first cavity 30, the driving device 7 drives the sampling needle 1 to move to above the part of the first cavity 30 added with the hemolytic agent and suck the liquid in the first cavity 30.
Specifically, in the present embodiment, the first cavity 30 may be a white blood cell/hemoglobin reaction cell 30a, a white blood cell four-classification reaction cell 30b, a nucleated red blood cell reaction cell 30c, and the first measurement system 5 connected to the first cavity 30 may be a white blood cell/hemoglobin measurement system 5a, a white blood cell four-classification measurement system 5b, a nucleated red blood cell measurement system 5c, and the like corresponding to the first cavity 30.
S205: the driving means 7 drives the sampling needle 1 to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle 1.
Specifically, in an application scenario, the swab 60 in the cleaning device 6 may be used to discharge the liquid containing the hemolytic agent component sucked in the sampling needle 1, the driving device 7 drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 of the swab 60 until the needle 10 of the sampling needle 1 is close to the liquid outlet 604, preferably, the driving device 7 drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 of the swab 60 until the needle 10 of the sampling needle 1 is flush with the liquid outlet 604, the driving device 7 flows out the liquid containing the hemolytic agent component in the sampling needle 1, and the liquid containing the hemolytic agent component flows into the liquid outlet pipe 64 through the liquid outlet 604.
S206: the inner and outer walls of the sampling needle 1 are further cleaned by means of a cleaning device 6.
Specifically, in an application scenario, before the step S206 is started, the driving device 7 drives the needle 10 of the sampling needle 1 to penetrate through and protrude from the swab 60, and the step S206 specifically includes: the driving device 7 drives the sampling needle 1 to move up and down along the swab 60, the diluent is sprayed to the outer wall of the sampling needle 1 after passing through the liquid inlet pipeline 62 to the liquid inlet 602, so that the outer wall of the sampling needle 1 is cleaned, and the cleaned liquid flows into the liquid outlet pipeline 64 to the waste liquid cavity 8 after passing through the liquid outlet 604. The driving device 7 further drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 until the needle 10 of the sampling needle 1 is close to the liquid outlet 604, preferably, the driving device 7 drives the needle 10 of the sampling needle 1 to move towards the liquid outlet 604 of the swab 60 until the needle 10 of the sampling needle 1 is flush with the liquid outlet 604, the driving device 7 makes the diluent in the sampling needle 1 flow out to clean the inner wall of the sampling needle 1, and the cleaned liquid flows into the liquid outlet pipeline 64 after passing through the liquid outlet 604.
S207: the drive means 7 drives the sampling needle 1 back to the initial position.
Of course, in other embodiments, the step S206 may be omitted, and the omitted method flow is included in the scope of the present application.
In general, the concentration of normal red blood cells is several hundred times higher than that of white blood cells, and therefore, the sample in the red blood cell/platelet count cell 32a can be obtained by performing a secondary dilution by sucking a sample, to which a hemolytic agent has not been added, in the white blood cell/hemoglobin reaction cell 30 a. The process of adding the sample in the red blood cell/platelet count cell 32a may be referred to as secondary sample addition, and the following two methods are provided in the present application for the method of cleaning the sampling needle 1 in the secondary sample addition process.
Fig. 6 is a schematic flow chart of another embodiment of the cleaning method for a sampling needle according to the present application, and the method includes:
s301: the driving device 7 drives the sampling needle 1 to suck the first sample stored in the sample tube 2 from the initial position to the sample tube 2.
Specifically, this step is the same as step S201 in the above embodiment, and is not described herein again.
S302: the outer wall of the sampling needle 1 is cleaned by means of a cleaning device 6.
Specifically, the step is the same as step S202 in the above embodiment, and is not described herein again.
S303: the driving device 7 drives the sampling needle 1 to add the first sample into the first cavity 30 to form a second sample, wherein a part of the first cavity is not added with the hemolytic agent.
Specifically, in an application scenario, the first cavity 30 includes a leukocyte/hemoglobin reaction cell 30a, a leukocyte four-classification reaction cell 30b, a nucleated red blood cell reaction cell 30c, and the like. In one embodiment, before or after the step S303, the method provided by the present application further includes: a certain proportion of diluent is added to the first cavity 30, and a certain proportion of hemolytic agent is added to a part of the first cavity 30. In an application scenario, the first cavity 30 may include a leukocyte four-classification reaction cell 30b, a nucleated red blood cell reaction cell 30c, and a leukocyte/hemoglobin reaction cell cavity 30a, wherein a hemolytic agent is not added in the leukocyte/hemoglobin reaction cell cavity 30 a. Of course, the hemolytic agent may not be added in other reaction tanks, as long as it is ensured that at least one chamber in the first chamber 30 is not added with hemolytic agent. The second sample is a liquid sample formed after the first sample and the diluent.
S304: during the operation of the first measuring system 5 connected with the part of the first cavity 30 added with the hemolytic agent, the driving device 7 drives the sampling needle 1 to move to the position above the part of the first cavity 30 added with the hemolytic agent and suck the liquid in the first cavity 30.
Specifically, in the present embodiment, the first measurement system 5 connected to the first cavity 30 may be the leukocyte four-classification measurement system 5b and the nucleated red blood cell measurement system 5c corresponding to the step S303.
S305: the driving means 7 drives the sampling needle 1 to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle 1.
Specifically, the step is the same as step S205 in the above embodiment, and is not described herein again.
S306: the inner and outer walls of the sampling needle 1 are further cleaned by means of a cleaning device 6.
Specifically, this step is the same as step S206 in the above embodiment, and is not described herein again.
S307: the driving device 7 drives the sampling needle 1 to draw the second sample from the part of the first cavity 30 to which the hemolytic agent is not added into the second cavity, wherein the second cavity comprises the erythrocyte/platelet counting cell.
S308: the inner and outer walls of the sampling needle 1 are cleaned by means of a cleaning device 6.
Specifically, this step is the same as step S206 in the above embodiment, and is not described herein again.
S309: the drive means 7 drives the sampling needle 1 back to the initial position.
Of course, in other embodiments, the step S306 may be omitted, and the omitted method flow is included in the scope of the present application.
Fig. 7 is a schematic flow chart of another embodiment of the cleaning method for the sampling needle of the present application, and the method includes:
s401: the driving device 7 drives the sampling needle 1 to suck the first sample stored in the sample tube 2 from the initial position to the sample tube 2.
Specifically, this step is the same as step S301 in the above embodiment, and is not described herein again.
S402: the outer wall of the sampling needle 1 is cleaned by means of a cleaning device 6.
Specifically, the step is the same as step S302 in the above embodiment, and is not described herein again.
S403: the driving device 7 drives the sampling needle 1 to add the first sample into the first cavity 30 to form a second sample, wherein a part of the first cavity 30 is not added with the hemolytic agent.
Specifically, this step is the same as step S303 in the above embodiment, and is not described herein again.
S404: the inner and outer walls of the sampling needle 1 are cleaned by means of a cleaning device 6.
Specifically, the step is the same as step S306 or S308 in the above embodiment, and is not described again here.
S405: the driving device 7 drives the sampling needle 1 to draw the second sample from the part of the first cavity 30 to which the hemolytic agent is not added into the second cavity, wherein the second cavity comprises the erythrocyte/platelet counting cell.
Specifically, the step is the same as step S307 in the above embodiment, and is not described herein again. For example, the first chamber 30 to which no hemolytic agent is added may be the leukocyte/hemoglobin reaction cell 30 a.
S406: a hemolytic agent is added to the first chamber 30 in the chamber where no hemolytic agent is added.
Specifically, in the present embodiment, the first cavity 30 includes a leukocyte/hemoglobin reaction chamber 30a, a leukocyte four-classification reaction chamber 30b, or a nucleated red blood cell reaction chamber 30c, and a hemolytic agent is added in the first cavity 30.
S407: during the operation of the first measuring system 5 connected to the first cavity 30, the driving device 7 drives the sampling needle 1 to move above the first cavity 30 and suck the liquid in the first cavity 30, wherein the first cavity 30 contains the hemolytic agent.
Specifically, the step is similar to the step S304 in the above embodiment, and is not described herein again.
S408: the driving means 7 drives the sampling needle 1 to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle 1.
Specifically, the step is the same as step S305 in the above embodiment, and is not described herein again.
S409: the inner and outer walls of the sampling needle 1 are further cleaned by means of a cleaning device 6.
Specifically, the step is the same as step S308 in the above embodiment, and is not described herein again.
S410: the drive means 7 drives the sampling needle 1 back to the initial position.
Specifically, this step is the same as step S309 in the above embodiment, and is not described herein again.
Of course, in other embodiments, at least one of the steps S404, S406, and S409 may be omitted, and the omitted method flow is included in the scope of the present application.
In general, the step of washing the sampling needle 1 with the liquid containing the hemolytic agent component in the first method occurs before the second pipetting, and the step of washing the sampling needle 1 with the liquid containing the hemolytic agent component in the second method occurs after the second pipetting. Of course, in other embodiments, the step of washing the sampling needle 1 with a liquid containing a hemolytic agent component is employed both before and after the second pipetting.
In summary, unlike the prior art, the blood cell analyzer provided in the present application drives the sampling needle 1 to move above the first cavity 30 containing the liquid of the hemolytic component and suck and spit the liquid in the first cavity 30 during the operation of the first measurement system 5 connected to the first cavity 30. Compared with the traditional method of utilizing diluent to clean, the liquid containing hemolytic agent components can clean residues such as lipid or protein on the inner wall of the sampling needle 1, the cleaning effect is good, and the problem that counting of red blood cells, platelets and the like is abnormally high due to the fact that the diluent is not cleaned completely can be avoided. Compare with current cleaning solution washing that utilizes, commonly used hemolytic agent washs in this application utilizes the blood cell analysis appearance, need not professional cleaning solution, and the washing cost is lower.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (10)
1. A method of cleaning a sampling needle for use with a blood cell analyzer, the method comprising:
in the working process of a first measuring system connected with a first cavity, a driving device drives a sampling needle to move above the first cavity and suck liquid in the first cavity, wherein the liquid is liquid containing hemolytic agent components;
the driving means drives the sampling needle to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle.
2. The cleaning method according to claim 1,
the first cavity comprises at least one of a leukocyte/hemoglobin reaction tank, a leukocyte four-classification reaction tank and a nucleated erythrocyte reaction tank, and the liquid containing the hemolytic agent component is the liquid obtained after the sample reacts with the hemolytic agent.
3. The cleaning method according to claim 2,
the first measurement system comprises a leukocyte/hemoglobin measurement system and/or a leukocyte four-classification measurement system and/or a nucleated red blood cell measurement system corresponding to the first cavity.
4. The cleaning method according to claim 1,
in the working process of a first measuring system connected with a first cavity, a driving device drives a sampling needle to move above the first cavity and suck liquid in the first cavity, and before the working process, the cleaning method comprises the following steps: the driving device drives the sampling needle to add the first sample into a plurality of cavities, wherein the plurality of cavities comprise a first cavity and a second cavity, and the second cavity comprises a red blood cell/platelet counting cell.
5. The cleaning method according to claim 1, wherein during the operation of the first measurement system connected with the first cavity, the driving device drives the sampling needle to move above the first cavity and suck the liquid in the first cavity, and before the cleaning method comprises the following steps: the driving device drives the sampling needle to add a first sample into the first cavity to form a second sample, wherein a part of the first cavity is not added with a hemolytic agent;
the driving device drives the sampling needle to suck the second sample from the first cavity without the hemolytic agent added into the second cavity, wherein the second cavity comprises a red blood cell/platelet counting pool.
6. The cleaning method according to claim 1,
in the working process of a first measuring system connected with a first cavity, a driving device drives a sampling needle to move above the first cavity and suck liquid in the first cavity, and before the working process, the cleaning method comprises the following steps: the driving device drives the sampling needle to add the first sample into the first cavity to form a second sample, wherein a part of the first cavity is not added with a hemolytic agent;
the driving means drives the sampling needle to discharge the aspirated liquid containing the hemolytic agent component to wash the sampling needle, and thereafter, the washing method includes: the driving device drives the sampling needle to suck the second sample from the first cavity without the hemolytic agent added into the second cavity, wherein the second cavity comprises a red blood cell/platelet counting pool.
7. The cleaning method according to claim 1,
the blood cell analyzer further comprises a cleaning device, wherein the cleaning device comprises a swab, a liquid inlet pipeline and a liquid outlet pipeline and is used for further cleaning the inner wall and the outer wall of the sampling needle;
the swab comprises a body provided with a containing cavity, and a liquid inlet and a liquid outlet which are positioned on the side wall of the body and communicated with the containing cavity, wherein the liquid outlet is positioned above the liquid inlet; the liquid inlet pipeline is connected with the liquid inlet so that diluent enters the cavity through the liquid inlet pipeline; the liquid outlet pipeline is connected with the liquid outlet so as to enable the liquid in the containing cavity to flow out.
8. The washing method according to claim 7, wherein the driving means drives the sampling needle to discharge the aspirated liquid containing the hemolytic agent component includes:
the driving device drives the needle head of the sampling needle to move towards the liquid outlet until the needle head of the sampling needle is close to the liquid outlet, the driving device enables liquid containing hemolytic agent components in the sampling needle to flow out, and the liquid containing hemolytic agent components flows into the liquid outlet pipeline through the liquid outlet.
9. A hematology analyzer, comprising:
a sampling needle for aspirating a sample;
a plurality of chambers including a first chamber for containing a liquid containing a hemolytic agent component;
the first measuring system is connected with the first cavity and used for detecting liquid contained in the first cavity;
and the driving device is connected with one end of the sampling needle and used for driving the sampling needle to absorb the liquid containing hemolytic agent components in the first cavity in the working process of the first measuring system so as to clean the sampling needle.
10. The hematology analyzer of claim 9,
the first cavity comprises at least one of a leukocyte/hemoglobin reaction tank, a leukocyte four-classification reaction tank and a nucleated erythrocyte reaction tank, and the liquid containing the hemolytic agent component is the liquid obtained after the sample reacts with the hemolytic agent;
the first measurement system comprises a leukocyte/hemoglobin measurement system, a leukocyte four-classification measurement system and a nucleated red blood cell measurement system which correspond to the first cavity.
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| CN201811012023.9A CN110871202A (en) | 2018-08-31 | 2018-08-31 | Sampling needle cleaning method and blood cell analyzer |
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| CN201811012023.9A CN110871202A (en) | 2018-08-31 | 2018-08-31 | Sampling needle cleaning method and blood cell analyzer |
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