CN105699635A - Simple and reliable hemocyte five-class analysis method, system and device - Google Patents
Simple and reliable hemocyte five-class analysis method, system and device Download PDFInfo
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Abstract
The invention discloses a simple and reliable hemocyte five-class analysis method, system and device. The system comprises a white blood cell counting chamber, an erythrocyte and blood platelet counting chamber, a five-class detecting device, a hemoglobin detecting device and an erythrocyte detecting device, wherein a blood dividing device and a reagent distributing device are respectively used for injecting the blood sample and the corresponding reagent into the two counting chambers for reaction. The system is characterized in that two hemolytic agents, namely a first hemolytic agent and a second hemolytic agent, are adopted; the white blood cell four-class counting and the basicyte counting are respectively measured in two times by the five-class detecting device; the first hemolytic agent and the blood sample are mixed for realizing the white blood cell four-class counting and the total amount counting of white blood cell; the second hemolytic agent and the blood sample added with a diluent are mixed for realizing the basicyte counting. According to the system provided by the invention, low cost and small volume are realized, the accuracy of basicyte can reach the level of high-end five-class blood cell analyzer and the system is more fit for primary hospitals.
Description
Technical field
The present invention relates to blood cell analysis field, in particular a kind of simple and reliable hemocyte five classifying and analyzing method, system and device。
Background technology
Hemocyte in blood of human body includes the compositions such as leukocyte, erythrocyte, platelet, wherein leukocyte is divided into again lymphocyte, mononuclear cell, basophil, neutrophilic granulocyte, this five classes leukocyte of eosinophilic granulocyte, and in hospital, blood routine examination is the inspection project that hemocyte in blood is counted。Before owing to technology is limited, cellanalyzer fails five class leukocyte are carried out Accurate classification counting, leukocyte can only be divided into three groups: lymphocyte populations, intermediate group cell, neutrophilic granulocyte group, fail to be supplied to the comprehensive diagnostic message of doctor and inconvenient doctor carries out clinical diagnosis。Therefore the cellanalyzer that can carry out leukocyte five classification is more aobvious important。
The analytical plan that current five classification cellanalyzers adopt has:
Scheme one: referring to Fig. 1, instrument contains four differential counting ponds 4, basophil counting chamber 5, hemoglobinometry pond 6, erythrocyte and platelet count pond 7, by a point blood device, blood sample is divided into respectively each counting chamber and measuring cell after blood sampling, it is subsequently adding corresponding reaction reagent to react, after having reacted, in four differential counting ponds, sample feeding detecting device 1 is counted by laser scattering method, in basophil counting chamber, sample is also fed into detecting device 1 and is counted by laser scattering method, in hemoglobinometry pond, sample is measured by detecting device 2, erythrocyte and sample in platelet count pond are sent into detecting device 3 and are carried out erythrocyte and platelet count by impedance method。Due to four differential countings and basophil counting common detection device 1, it is necessary to staggering time section counts, and total white blood cells can obtain from sample standard deviation in four differential counting ponds and basophil counting chamber。In four differential counting ponds, added hemolytic agent is the four independent special hemolytic agents 22 of differential counting, in basophil counting chamber, added hemolytic agent is that basophil counts independent special hemolytic agent 23, and in hemoglobinometry pond, added hemolytic agent is the independent special hemolytic agent 24 of hemoglobinometry。
The shortcoming of the program is to have employed four counting chambers, and machine system is complicated, and cost is high, and volume takes up room greatly greatly, it is difficult to cellanalyzer of the type five being classified is popularized for township hospital Ji Shekang center。
Scheme two: referring to Fig. 2, instrument contains four differential counting ponds 12, numeration of leukocyte pond 13, erythrocyte and platelet count pond 14, reacted to each counting chamber addition blood sample and corresponding reagent respectively by point blood device and reagent dispensing apparatus, in four differential counting ponds, sample is transported to detecting device 8 and carries out four differential countings by laser scattering method, in numeration of leukocyte pond, sample is used for carrying out total white blood cells counting, hemoglobinometry and basophil counting, wherein hemoglobinometry measures in detecting device 9, total white blood cells counting and basophil counting are sent into detecting device 10 and are counted by impedance method。Erythrocyte and sample input in platelet count pond to detecting device 11 carry out erythrocyte and platelet count by impedance method。In four differential counting ponds, added hemolytic agent is the four independent special hemolytic agents 25 of differential counting, and adding another kind of hemolytic agent 26 in numeration of leukocyte pond can count total white blood cells, hemoglobinometry and basophil simultaneously。
Though the shortcoming of the program is only with three counting chambers, system complexity is medium, and volume is bigger than normal, and therefore cost is also higher, and need to take bigger working top space, it is also difficult to cellanalyzer of the type five being classified is popularized for township hospital Ji Shekang center。Additionally basophil is counted by impedance method, compares and laser scattering method, and accuracy is poor。
Scheme three: referring to Fig. 3, instrument contains numeration of leukocyte pond 20, erythrocyte and platelet count pond 21, by point blood device with reagent dispensing apparatus is divided into blood sample to two counting chambers respectively and corresponding reagent reacts, in numeration of leukocyte pond, part sample is transported to detecting device 17 and is counted by laser scattering method, carry out total white blood cells counting simultaneously, four differential countings and basophil counting, in numeration of leukocyte pond, part sample carries out hemoglobinometry at detecting device 18, erythrocyte carries out erythrocyte and platelet count with sample input in platelet count pond to detecting device 19。Numeration of leukocyte pond only adds a kind of hemolytic agent 27 to realize total white blood cells counting, four differential countings, basophil counting, hemoglobinometry。
Program system is the simplest, volume is also minimum, it is suitable for suitable in township hospital Ji Shekang center, but owing to only adding a kind of hemolytic agent to realize total white blood cells counting, four differential countings, basophil counting, hemoglobinometry, a kind of scatterplot can only be got by laser scattering method, in this scatterplot, four classification leucocytes and basophil do not have boundary clearly, basophil can only incorporate experience into Preliminary Determination count results, therefore basophil poor accuracy, especially special sample, its accuracy is relatively difficult to ensure card。
Therefore, prior art has yet to be improved and developed。
Summary of the invention
It is an object of the invention to provide a kind of simple and reliable hemocyte five classifying and analyzing method, system and device, it is intended to solve existing system complexity, volume is big, cost is high, and the problem of the poor accuracy of basophil。
Technical scheme is as follows:
A kind of simple and reliable hemocyte five classifying and analyzing method, it comprises the following steps:
S1: draw sample from machine by a point blood device；
S2: emptying numeration of leukocyte pond, adds the first hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond；
S3: by point blood device, the part sample of absorption is added numeration of leukocyte pond and react with the first hemolytic agent；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveyer device and carries out leukocyte four differential counting；
S5: emptying numeration of leukocyte pond again, then passes through cleanout fluid and numeration of leukocyte pond is carried out；
S6: add diluent by reagent dispensing apparatus toward numeration of leukocyte pond, then again through point blood device, the part sample drawn is added numeration of leukocyte pond, form mixed liquor；
S8: after having drawn part mixed liquor, adds the second hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond and reacts；Reacted solution is sent into five classification and Detection devices by conveyer device and can carry out basophil counting, complete five classification and Detection。
Described method, wherein, can count total white blood cells while carrying out four differential countings in step s 4。
Described method, wherein, counts total white blood cells while carrying out basophil counting in step s 8。
Described method, wherein, after carrying out step S6, can will carry out step S7: by dividing blood device to draw part mixed liquor from numeration of leukocyte pond, then mixed liquor is added erythrocyte and platelet count pond, is carried out erythrocyte and platelet count by erythrocyte detecting device。
Described method, wherein, is simultaneously fed into hemoglobin detection device by solution reacted in step S8 by conveyer device and carries out hemoglobinometry。
A kind of simple and reliable hemocyte five classification analysis system, including numeration of leukocyte pond, erythrocyte and platelet count pond, five classification and Detection devices, hemoglobin detection device, erythrocyte detecting device, by point blood device and reagent dispensing apparatus respectively to two counting chambers being divided into blood sample and corresponding reagent reacts, its system is provided with two kinds of hemolytic agents, respectively the first hemolytic agent and the second hemolytic agent, and leukocyte four differential counting measures respectively through five classification and Detection devices at twice with basophil counting, mixed with blood sample by the first hemolytic agent and realize leukocyte four differential counting and total white blood cells counting；Mixed with the blood sample being added with diluent by the second hemolytic agent and realize basophil counting。
Described system, wherein, total white blood cells counts and four differential countings or basophil count synchronization。
Described system, wherein, adopts the blood sample of direct mixed diluting liquid to be realized erythrocyte and platelet count by erythrocyte detecting device。
Described system, wherein, adopts hemoglobin detection device that the sample in numeration of leukocyte pond is carried out hemoglobinometry while basophil counts。
A kind of simple and reliable hemocyte five classification analysis device, wherein, including sampling needle, erythrocyte detecting device, erythrocyte and platelet count pond, hemoglobin detection device, numeration of leukocyte pond, diluent, first hemolytic agent, second hemolytic agent, five classification and Detection devices, tapping equipment and conveyor module, described sampling needle connects conveyor module, sample sampling and distribution has been coordinated by conveyor module, described erythrocyte detecting device connects erythrocyte and platelet count pond, described hemoglobin detection device connects numeration of leukocyte pond, described diluent connects numeration of leukocyte pond, erythrocyte and platelet count pond and conveyor module, described first hemolytic agent connects numeration of leukocyte pond and conveyor module, described second hemolytic agent connects numeration of leukocyte pond and conveyor module, described five classification and Detection devices connect conveyor module, described tapping equipment connects numeration of leukocyte pond and erythrocyte and platelet count pond。
Beneficial effects of the present invention: the present invention, by adopting leukocyte Double channel scheme, has independent hemolytic agent that leukocyte is processed, it is possible to basophil difference widens, better assures that the count results of basophil；Leukocyte four classifies hemolytic agent that passage uses and the hemolytic agent that basophil classification passage uses does not intersect mixing each other, independent reaction, will not have influence on the classification stability of each classification passage because mixing intersection；Leukocyte four classify passage, basophil classification passage share a numeration of leukocyte pond, and common detection device, counting chamber used by hemoglobinometry still shares same counting chamber with upper two passages simultaneously, whole system is simple and reliable, cost is low, volume is little, and the accuracy of basophil can reach high-end five classification cellanalyzer levels, is more suitable for basic hospital and is used。
Accompanying drawing explanation
Fig. 1 is existing hemocyte five classification analysis system block diagram；
Fig. 2 is existing hemocyte five classification analysis system block diagram；
Fig. 3 is existing hemocyte five classification analysis system block diagram；
Fig. 4 is hemocyte five classification analysis system block diagram provided by the invention；
Fig. 5 is hemocyte five classifying and analyzing method flow chart provided by the invention；
Fig. 6 is hemocyte five classification analysis apparatus structure schematic diagram provided by the invention。
Detailed description of the invention
For making the purpose of the present invention, technical scheme and advantage clearly, clearly, developing simultaneously referring to accompanying drawing, the present invention is described in more detail for embodiment。
Referring to Fig. 4, the system of the present invention contains numeration of leukocyte pond, erythrocyte and platelet count pond, five classification and Detection devices, hemoglobin detection device, erythrocyte detecting device, by point blood device (not shown) and reagent dispensing apparatus (not shown) respectively to being divided into blood sample in two counting chambers and corresponding reagent reacts。This system and existing system are distinctive in that and are provided with two kinds of hemolytic agents, respectively the first hemolytic agent and the second hemolytic agent, and leukocyte four differential counting carries out respectively through five classification and Detection devices at twice with basophil counting；Total white blood cells counting can with four differential countings or basophil count synchronization。Mixed with blood sample by the first hemolytic agent and realize leukocyte four differential counting and total white blood cells counting；Mixed with the blood sample being added with diluent by the second hemolytic agent and realize basophil counting；The blood sample adopting direct mixed diluting liquid realizes erythrocyte and platelet count by erythrocyte detecting device；Adopt hemoglobin detection device that the sample in numeration of leukocyte pond is carried out hemoglobinometry while basophil counts。
Referring to Fig. 5, the flow process of method provided by the invention is as follows:
S1: draw sample from machine by a point blood device；
S2: emptying numeration of leukocyte pond, adds the first hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond；
S3: by point blood device, the part sample of absorption is added numeration of leukocyte pond and react with the first hemolytic agent；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveyer device and carries out leukocyte four differential counting, total white blood cells can be counted simultaneously；
S5: emptying numeration of leukocyte pond again, then passes through cleanout fluid and numeration of leukocyte pond is carried out；
S6: add diluent by reagent dispensing apparatus toward numeration of leukocyte pond, then again through point blood device, the part sample drawn is added numeration of leukocyte pond, form mixed liquor；
S7: draw part mixed liquor by point blood device from numeration of leukocyte pond, then mixed liquor is added erythrocyte and platelet count pond；
S8: after having drawn part mixed liquor, adds the second hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond and reacts；Reacted solution is sent into by conveyer device hemoglobin detection device and five classification and Detection devices；
S9: carry out three countings parallel: being carried out erythrocyte and platelet count by erythrocyte detecting device, carried out hemoglobinometry by hemoglobin detection device in numeration of leukocyte pond and carry out basophil counting by five classification and Detection devices, total white blood cells can be counted by while；
S10: finally all results are calculated, and export each cytometry result。
Wherein, if only completing five classification and Detection, method step is as follows:
S1: draw sample from machine by a point blood device；
S2: emptying numeration of leukocyte pond, adds the first hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond；
S3: by point blood device, the part sample of absorption is added numeration of leukocyte pond and react with the first hemolytic agent；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveyer device and carries out leukocyte four differential counting；
S5: emptying numeration of leukocyte pond again, then passes through cleanout fluid and numeration of leukocyte pond is carried out；
S6: add diluent by reagent dispensing apparatus toward numeration of leukocyte pond, then again through point blood device, the part sample drawn is added numeration of leukocyte pond, form mixed liquor；
S8: after having drawn part mixed liquor, adds the second hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond and reacts；Reacted solution is sent into five classification and Detection devices by conveyer device and can carry out basophil counting, complete five classification and Detection。
Further:
Total white blood cells can be counted while carrying out four differential countings in step s 4；Also while can carrying out basophil counting in step s 8, total white blood cells is counted。
After carrying out step S6, can will carry out step S7 flow process, it may be assumed that draw part mixed liquor by point blood device from numeration of leukocyte pond, then mixed liquor is added erythrocyte and platelet count pond, is carried out erythrocyte and platelet count by erythrocyte detecting device。
Only reacted solution in step S8 need to be added in hemoglobin detection device when needing to measure hemoglobin。
By point blood device by after the part sample drawn and the first hemolytic agent hybrid reaction, erythrocyte is dissolved, lymphocyte in leukocyte, mononuclear cell, neutrophilic granulocyte, eosinophilic granulocyte have obvious difference characteristic, pass through laser scattering method, leukocyte four can be classified, also leukocyte can be carried out total counting number simultaneously。
React after numeration of leukocyte pond adds diluent, part blood sample and the second hemolytic agent, after reaction, erythrocyte is dissolved, leukocyte medium-sized lymphocyte, mononuclear cell, neutrophilic granulocyte, eosinophilic granulocyte are processed into same feature, and basophil and this four classes leukocyte have obvious difference characteristic and can accurately make a distinction counting, therefore by laser scattering method, basophil can be carried out accurate counting, also leukocyte can be carried out total counting number simultaneously。
Add hemolytic agent reaction treatment by twice, carry out leukocyte four differential counting and basophil counting respectively, thus exactly to leukocyte five differential counting。
As shown in Figure 6, the present invention provides a kind of preferred device to implement said method, and a kind of simple and reliable hemocyte five sorter includes sampling needle 41, erythrocyte detecting device 42, erythrocyte and platelet count pond 43, hemoglobin detection device 44, numeration of leukocyte pond 45, diluent the 46, first hemolytic agent the 47, second hemolytic agent 48, five classification and Detection device 49, tapping equipment 50 and conveyor module 51。
Described sampling needle 41 is used for taking sample and distribution sample, connects conveyor module 51, has been coordinated sample sampling and distribution by conveyor module 51。
Described erythrocyte detecting device 42 connects erythrocyte and platelet count pond 43, is used for erythrocyte and platelet are counted。Described erythrocyte is used for the place providing erythrocyte to mix with diluent with platelet with platelet count pond 43。
Described hemoglobin detection device 44 connects numeration of leukocyte pond 45, is used for measuring hemoglobin。Described numeration of leukocyte pond 45 is used for providing the reaction tank needed for leukocyte four classification reaction, basophil classification reaction and hemoglobinometry。
Described diluent 46 connects numeration of leukocyte pond 45, erythrocyte and platelet count pond 43 and conveyor module 51, for diluted sample and washer parts。
Described first hemolytic agent 47 connects numeration of leukocyte pond 45 and conveyor module 51, for lysed erythrocyte, the lymphocyte in leukocyte, mononuclear cell, neutrophilic granulocyte, eosinophilic granulocyte four class cell is pulled open difference and is conveniently used for four differential countings。
Described second hemolytic agent 48 connects numeration of leukocyte pond 45 and conveyor module 51, for lysed erythrocyte (new blend of generation can be utilized for hemoglobinometry), basophil in leukocyte is carried out specific process, other four classes leukocyte and basophil and notable difference, thus realizing the differential counting of basophil。
Described five classification and Detection devices 49 connect conveyor module 51, are devices leukocyte classified (including leukocyte four classification and basophil classification) by laser scattering method。
Described tapping equipment 50 connects numeration of leukocyte pond 45 and erythrocyte and platelet count pond 43, is used for discharging the waste liquid that counting process produces；Described conveyor module 51 is used for drawing sample, distribution sample, distribution diluent, distribution hemolytic agent, conveying detection sample enter five classification and Detection devices 49 and detect。
Wherein, described conveyor module 51 can be distributed by syringe or dosing pump or carry respective sample, it is possible to the mode being combined by different number of syringe and dosing pump is realized。
The detection process of this device is as follows:
1) quantitative sample is drawn by conveyor module 50 and sampling needle 41；
2) by tapping equipment 50, liquid in numeration of leukocyte pond 45 is drained, then again through conveyor module 51, first hemolytic agent 47 is added numeration of leukocyte pond 50；
3) divide sample to enter numeration of leukocyte pond 45 by conveyor module 51 and sampling needle 41 branch and the first hemolytic agent 47 carries out hybrid reaction；
4), after reaction certain time, by conveyor module 51, mixed liquor is delivered to detecting device 49 and carries out leukocyte four differential counting, thus lymphocyte, mononuclear cell, neutrophilic granulocyte, eosinophilic granulocyte are carried out accurate counting；
5) by tapping equipment 50, remaining mixed liquor in numeration of leukocyte pond 45 is emptied；
6) by conveyor module 51, diluent 46 is added numeration of leukocyte pond 45, then again through conveyor module 51 and sampling needle 41, sample is divided into numeration of leukocyte pond and mixes；
7) extract part mixing sample by conveyor module 51 and sampling needle 41 from numeration of leukocyte pond 45 after having mixed, then this part mixing sample is divided into erythrocyte and platelet count pond 43；
9) in remaining mixing sample in numeration of leukocyte pond 45, add the second hemolytic agent 48 to react；
10) after reaction, carry out hemoglobinometry by detecting device 44, and by conveyor module 51, reacted mixing sample is delivered to detecting device 49 and carries out basophil differential counting；Meanwhile by detecting device 42, erythrocyte and platelet are counted；
11) complete all countings, export result。
Total white blood cells counting can pass through step 4 or step 10, and these two steps all can obtain total white blood cells result。
The present invention, by adopting leukocyte Double channel scheme, has independent hemolytic agent that leukocyte is processed, it is possible to basophil difference widens, better assures that the count results of basophil；Leukocyte four classifies hemolytic agent that passage uses and the hemolytic agent that basophil classification passage uses does not intersect mixing each other, independent reaction, will not have influence on the classification stability of each classification passage because mixing intersection；Leukocyte four classify passage, basophil classification passage share a numeration of leukocyte pond, and common detection device, counting chamber used by hemoglobinometry still shares same counting chamber with upper two passages simultaneously, whole system is simple and reliable, cost is low, volume is little, and the accuracy of basophil can reach high-end five classification cellanalyzer levels, is more suitable for basic hospital and is used。
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, it is possible to improved according to the above description or convert, all these improve and convert the protection domain that all should belong to claims of the present invention。
Claims (10)
1. hemocyte five classifying and analyzing method one kind simple and reliable, it is characterised in that comprise the following steps:
S1: draw sample from machine by a point blood device；
S2: emptying numeration of leukocyte pond, adds the first hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond；
S3: by point blood device, the part sample of absorption is added numeration of leukocyte pond and react with the first hemolytic agent；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveyer device and carries out leukocyte four differential counting；
S5: emptying numeration of leukocyte pond again, then passes through cleanout fluid and numeration of leukocyte pond is carried out；
S6: add diluent by reagent dispensing apparatus toward numeration of leukocyte pond, then again through point blood device, the part sample drawn is added numeration of leukocyte pond, form mixed liquor；
S8: after having drawn part mixed liquor, adds the second hemolytic agent by reagent dispensing apparatus toward numeration of leukocyte pond and reacts；Reacted solution is sent into five classification and Detection devices by conveyer device and can carry out basophil counting, complete five classification and Detection。
2. method according to claim 1, it is characterised in that total white blood cells can be counted while carrying out four differential countings in step s 4。
3. method according to claim 1, it is characterised in that while carrying out basophil counting in step s 8, total white blood cells is counted。
4. method according to claim 1, it is characterized in that, after carrying out step S6, can will carry out step S7: by dividing blood device to draw part mixed liquor from numeration of leukocyte pond, then mixed liquor is added erythrocyte and platelet count pond, is carried out erythrocyte and platelet count by erythrocyte detecting device。
5. method according to claim 1, it is characterised in that solution reacted in step S8 is simultaneously fed into hemoglobin detection device by conveyer device and carries out hemoglobinometry。
6. hemocyte five classification analysis system one kind simple and reliable, including numeration of leukocyte pond, erythrocyte and platelet count pond, five classification and Detection devices, hemoglobin detection device, erythrocyte detecting device, by point blood device and reagent dispensing apparatus respectively to two counting chambers being divided into blood sample and corresponding reagent reacts, it is characterized in that, system is provided with two kinds of hemolytic agents, respectively the first hemolytic agent and the second hemolytic agent, and leukocyte four differential counting measures respectively through five classification and Detection devices at twice with basophil counting, mixed with blood sample by the first hemolytic agent and realize leukocyte four differential counting and total white blood cells counting；Mixed with the blood sample being added with diluent by the second hemolytic agent and realize basophil counting。
7. system according to claim 6, it is characterised in that total white blood cells counting and four differential countings or basophil count synchronization。
8. system according to claim 7, it is characterised in that adopt the blood sample of direct mixed diluting liquid to be realized erythrocyte and platelet count by erythrocyte detecting device。
9. system according to claim 8, it is characterised in that adopt hemoglobin detection device that the sample in numeration of leukocyte pond is carried out hemoglobinometry while basophil counts。
10. hemocyte five classification analysis device one kind simple and reliable, it is characterized in that, including sampling needle, erythrocyte detecting device, erythrocyte and platelet count pond, hemoglobin detection device, numeration of leukocyte pond, diluent, first hemolytic agent, second hemolytic agent, five classification and Detection devices, tapping equipment and conveyor module, described sampling needle connects conveyor module, sample sampling and distribution has been coordinated by conveyor module, described erythrocyte detecting device connects erythrocyte and platelet count pond, described hemoglobin detection device connects numeration of leukocyte pond, described diluent connects numeration of leukocyte pond, erythrocyte and platelet count pond and conveyor module, described first hemolytic agent connects numeration of leukocyte pond and conveyor module, described second hemolytic agent connects numeration of leukocyte pond and conveyor module, described five classification and Detection devices connect conveyor module, described tapping equipment connects numeration of leukocyte pond and erythrocyte and platelet count pond。
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