CN111707601A - Whole blood biochemical detection method and device - Google Patents
Whole blood biochemical detection method and device Download PDFInfo
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- CN111707601A CN111707601A CN202010582419.8A CN202010582419A CN111707601A CN 111707601 A CN111707601 A CN 111707601A CN 202010582419 A CN202010582419 A CN 202010582419A CN 111707601 A CN111707601 A CN 111707601A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 59
- 239000008280 blood Substances 0.000 title claims abstract description 59
- 238000001514 detection method Methods 0.000 title claims abstract description 42
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 28
- 239000012895 dilution Substances 0.000 claims abstract description 25
- 238000010790 dilution Methods 0.000 claims abstract description 25
- 238000012360 testing method Methods 0.000 claims abstract description 20
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 13
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 13
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 11
- 238000002834 transmittance Methods 0.000 claims description 9
- 241000190070 Sarracenia purpurea Species 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 3
- 239000003219 hemolytic agent Substances 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 230000007547 defect Effects 0.000 abstract description 2
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/018—Platelets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1022—Measurement of deformation of individual particles by non-optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
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- General Health & Medical Sciences (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of medical detection, and particularly relates to a whole blood biochemical detection method, which specifically comprises the following steps: sucking a whole blood sample and physiological saline into a dilution container and conveying the whole blood sample and the physiological saline into a counting cup; constant current is conducted between two electrodes in the counting cup, resistance between the electrodes changes under the condition of constant negative pressure, so that pulse signals are generated, the pulse signals are collected by the detection unit, and red blood cells and platelets are classified and counted respectively; sucking whole blood sample and physiological saline and conveying the whole blood sample and the physiological saline into a colorimetric pool; a light source emitter irradiates a light source to a test sample in a colorimetric pool, then a detector respectively detects the fluorescence intensity and the scattered light intensity emitted by the test blood sample under the irradiation of the light source, and white blood cells in the test blood sample are counted and classified; the defect of the prior art is overcome, the dual-channel detection equipment is designed, red blood cells, platelets and white blood cells can be detected simultaneously, the detection efficiency is improved, and the treatment of a patient is facilitated.
Description
Technical Field
The invention belongs to the technical field of medical detection, and particularly relates to a whole blood biochemical detection method and a whole blood biochemical detection device.
Background
The biochemical analysis and detection of blood samples is an important index of clinical diagnosis, and the biochemical detection is required to be completed quickly under emergency situations in clinical work, so that precious time is saved for patients to cure.
The existing whole blood biochemical detection equipment generally adopts a single-channel method, only comprises one leukocyte classification detection channel, has low detection efficiency and influences the treatment of patients.
Disclosure of Invention
The invention aims to provide a whole blood biochemical detection method and a whole blood biochemical detection device, overcomes the defects of the prior art, designs a double-channel detection device, can simultaneously detect red blood cells, platelets and white blood cells, improves the detection efficiency, and is beneficial to the treatment of patients.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a whole blood biochemical detection method specifically comprises the following steps:
(1) preparing a blood sample: transferring a whole blood sample to be detected into a sample cup, adding a hemolytic agent into the whole blood sample, and simultaneously filling physiological saline into a physiological saline cup;
(2) sample feeding: sucking a certain amount of whole blood sample and physiological saline into a dilution container, uniformly mixing by vibration of a vibrator, and conveying to a counting cup;
(3) counting and detecting: constant current is conducted between two electrodes in the counting cup, resistance between the electrodes changes under the condition of constant negative pressure, so that pulse signals are generated, the detection unit collects the pulse signals, and the collected electric pulses are amplified and then compared with a channel voltage threshold value corresponding to a normal red blood cell/platelet volume range, so that red blood cells and platelets are classified and counted respectively;
(4) secondary sample introduction: after the sample introduction operation in the step (2) is finished, sucking a quantitative whole blood sample and physiological saline again, uniformly mixing the whole blood sample and the physiological saline through the vibration of the vibrator, and conveying the whole blood sample and the physiological saline into the colorimetric pool;
(5) colorimetric detection: the method comprises the following steps that a light source emitter irradiates a light source to a test sample in a colorimetric pool, then a transmittance turbidimetric detector and a dispersive turbidimetric detector respectively detect the fluorescence intensity and the scattered light intensity emitted by the test blood sample under the irradiation of the light source, and the white blood cells in the test blood sample are counted and classified according to the fluorescence intensity and the scattered light intensity emitted by the test blood sample respectively detected by the transmittance turbidimetric detector and the dispersive turbidimetric detector;
(6) and (4) outputting a result: the information of the detected red blood cells, platelets and white blood cells is classified and stored, and is output to a display device and a printing device.
Further, the dilution ratio of the whole blood sample to the physiological saline in the step (2) is 1:5-1: 20.
Further, the dilution ratio of the whole blood sample subjected to secondary sample injection in the step (4) to the physiological saline is 1:20-1: 100.
Further, in the step (4), before secondary sample injection, a certain amount of physiological saline is firstly absorbed, and the dilution cup is cleaned through vibration of the vibrator.
A whole blood biochemical detection device comprises a sample introduction system and a detection system;
the sample introduction system comprises a sample cup, a physiological salt water cup and a dilution cup with a vibrator, flow valves are arranged on connecting pipelines of the dilution cup, the sample cup and the physiological salt water cup, and a delivery pump assembly is arranged on an output pipeline of the dilution cup;
the detection system comprises a counting cup and a cuvette, wherein the counting cup is internally provided with two electrodes serving as a positive electrode and a negative electrode and a gem hole arranged between the two electrodes, the two sides of the cuvette are respectively provided with a light source emitter and a detector system, the detector system comprises a turbidimetric transmittance detector and a turbidimetric dispersion detector, and a stirrer is arranged in the cuvette.
Furthermore, a water outlet and a valve for discharging waste water are arranged on the dilution cup.
Further, still include control system and output system, output system includes display screen and printing device, control system is used for controlling opening and close of sampling system and detecting system.
Compared with the prior art, the invention has the following beneficial effects:
according to the whole blood biochemical detection method and device, the double-channel detection equipment is designed, so that the red blood cells, the platelets and the white blood cells can be detected simultaneously, the detection efficiency is improved, the treatment of a patient is facilitated, the design is novel, the operation is convenient and fast, and the practicability is strong.
Drawings
FIG. 1 is a schematic structural diagram of a whole blood biochemical detection device.
In the figure: 1. a sample cup; 2. a physiological saline cup; 3. a dilution cup; 4. a delivery pump assembly; 5. a counting cup; 51. an electrode; 52. a gem hole; 6. a cuvette; 61. a light source emitter; 62. a stirring assembly; 63. a detector system; 7. an output system; 8. and (5) controlling the system.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIG. 1, the whole blood biochemical detection device of the present invention comprises a sample introduction system and a detection system;
the sample introduction system comprises a sample cup 1, a physiological salt water cup 2 and a dilution cup 3 with a vibrator, flow valves are arranged on connecting pipelines of the dilution cup 3, the sample cup 1 and the physiological salt water cup 2, and a delivery pump assembly 4 is arranged on an output pipeline of the dilution cup 3;
the detection system comprises a counting cup 5 and a cuvette 6, wherein two electrodes 51 which are respectively used as a positive electrode and a negative electrode and a gem hole 52 arranged between the two electrodes 51 are arranged in the counting cup 5, a light source emitter 61 and a detector system 63 are respectively arranged on two sides of the cuvette 6, the detector system 63 comprises a transmittance turbidity detector and a dispersion turbidity detector, and a stirrer is arranged in the cuvette 6.
The dilution cup 3 is also provided with a water outlet and a valve for discharging waste water.
The automatic sample feeding device further comprises a control system 8 and an output system 7, wherein the output system 7 comprises a display screen and a printing device, and the control system 8 is used for controlling the opening and closing of the sample feeding system and the detection system.
The specific operation method of the equipment is as follows:
(1) preparing a blood sample: transferring a whole blood sample to be detected into a sample cup 1, adding a hemolytic agent into the whole blood sample, and simultaneously filling physiological saline into a physiological saline cup 2;
(2) sample feeding: sucking a whole blood sample and physiological saline into a dilution container according to a dilution ratio of 1:5-1:20, uniformly mixing by vibration of a vibrator, and conveying into a counting cup 5;
(3) counting and detecting: constant current is conducted between two electrodes 51 in the counting cup 5, resistance between the electrodes 51 is changed under the condition of constant negative pressure, so that pulse signals are generated, the detection unit collects the pulse signals, the collected electric pulses are amplified and then are compared with a channel voltage threshold corresponding to a normal red blood cell/platelet volume range, and classification and counting of red blood cells and platelets are realized;
(4) secondary sample introduction: after the sample introduction operation in the step (2) is finished, firstly absorbing quantitative physiological saline, cleaning the dilution cup 3 by the vibration of a vibrator, after the cleaned physiological saline is discharged, absorbing a whole blood sample and the physiological saline according to the dilution ratio of 1:20-1:100, uniformly mixing by the vibration of the vibrator, and conveying to a colorimetric pool;
(5) colorimetric detection: the light source emitter 61 irradiates a light source to a test sample in the cuvette, then the transmittance turbidimetric detector and the scattering turbidimetric detector respectively detect the fluorescence intensity and the scattered light intensity emitted by the test blood sample under the irradiation of the light source, and the white blood cells in the test blood sample are counted and classified according to the fluorescence intensity and the scattered light intensity emitted by the test blood sample respectively detected by the transmittance turbidimetric detector and the scattering turbidimetric detector;
(6) and (4) outputting a result: the information of the detected red blood cells, platelets and white blood cells is classified and stored, and is output to a display device and a printing device.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. A whole blood biochemical detection method is characterized in that: the method specifically comprises the following steps:
(1) preparing a blood sample: transferring a whole blood sample to be detected into a sample cup, adding a hemolytic agent into the whole blood sample, and simultaneously filling physiological saline into a physiological saline cup;
(2) sample feeding: sucking a certain amount of whole blood sample and physiological saline into a dilution container, uniformly mixing by vibration of a vibrator, and conveying to a counting cup;
(3) counting and detecting: constant current is conducted between two electrodes in the counting cup, resistance between the electrodes changes under the condition of constant negative pressure, so that pulse signals are generated, the detection unit collects the pulse signals, and the collected electric pulses are amplified and then compared with a channel voltage threshold value corresponding to a normal red blood cell/platelet volume range, so that red blood cells and platelets are classified and counted respectively;
(4) secondary sample introduction: after the sample introduction operation in the step (2) is finished, sucking a quantitative whole blood sample and physiological saline again, uniformly mixing the whole blood sample and the physiological saline through the vibration of the vibrator, and conveying the whole blood sample and the physiological saline into the colorimetric pool;
(5) colorimetric detection: the method comprises the following steps that a light source emitter irradiates a light source to a test sample in a colorimetric pool, then a transmittance turbidimetric detector and a dispersive turbidimetric detector respectively detect the fluorescence intensity and the scattered light intensity emitted by the test blood sample under the irradiation of the light source, and the white blood cells in the test blood sample are counted and classified according to the fluorescence intensity and the scattered light intensity emitted by the test blood sample respectively detected by the transmittance turbidimetric detector and the dispersive turbidimetric detector;
(6) and (4) outputting a result: the information of the detected red blood cells, platelets and white blood cells is classified and stored, and is output to a display device and a printing device.
2. The biochemical whole blood testing method according to claim 1, wherein: the dilution ratio of the whole blood sample to the physiological saline in the step (2) is 1:5-1: 20.
3. The biochemical whole blood testing method according to claim 1, wherein: the dilution ratio of the whole blood sample subjected to secondary sample introduction in the step (4) to the physiological saline is 1:20-1: 100.
4. The biochemical whole blood testing method according to claim 1 or 3, wherein: and (4) absorbing quantitative normal saline before secondary sample injection in the step (4), and cleaning the dilution cup through vibration of the vibrator.
5. A whole blood biochemical detection device is characterized in that: comprises a sample introduction system and a detection system;
the sample introduction system comprises a sample cup, a physiological salt water cup and a dilution cup with a vibrator, flow valves are arranged on connecting pipelines of the dilution cup, the sample cup and the physiological salt water cup, and a delivery pump assembly is arranged on an output pipeline of the dilution cup;
the detection system comprises a counting cup and a cuvette, wherein the counting cup is internally provided with two electrodes serving as a positive electrode and a negative electrode and a gem hole arranged between the two electrodes, the two sides of the cuvette are respectively provided with a light source emitter and a detector system, the detector system comprises a turbidimetric transmittance detector and a turbidimetric dispersion detector, and a stirrer is arranged in the cuvette.
6. The biochemical whole blood testing device according to claim 5, wherein: the diluting cup is also provided with a water outlet and a valve for discharging waste water.
7. The biochemical whole blood testing device according to claim 5, wherein: the automatic sample feeding device is characterized by further comprising a control system and an output system, wherein the output system comprises a display screen and a printing device, and the control system is used for controlling the opening and closing of the sample feeding system and the detection system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010582419.8A CN111707601A (en) | 2020-06-23 | 2020-06-23 | Whole blood biochemical detection method and device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010582419.8A CN111707601A (en) | 2020-06-23 | 2020-06-23 | Whole blood biochemical detection method and device |
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| CN111707601A true CN111707601A (en) | 2020-09-25 |
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| CN202010582419.8A Pending CN111707601A (en) | 2020-06-23 | 2020-06-23 | Whole blood biochemical detection method and device |
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Citations (8)
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| CN106404640A (en) * | 2016-10-28 | 2017-02-15 | 徐廷宽 | System for detecting and analyzing blood cells |
| EP3141885A1 (en) * | 2015-09-14 | 2017-03-15 | Sysmex Corporation | Blood analyzer and blood analyzing method |
| US20190049383A1 (en) * | 2017-08-10 | 2019-02-14 | Sysmex Corporation | Blood analyzer, blood analyzing method, and program |
-
2020
- 2020-06-23 CN CN202010582419.8A patent/CN111707601A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656499A (en) * | 1994-08-01 | 1997-08-12 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| CN1967244A (en) * | 2005-11-15 | 2007-05-23 | 希森美康株式会社 | Blood analyzer and blood analyzing method |
| CN103941026A (en) * | 2008-10-28 | 2014-07-23 | 希森美康株式会社 | Sample analyzer |
| CN105004641A (en) * | 2015-08-27 | 2015-10-28 | 苏州柯尔医疗器械有限公司 | Blood analyzer and analysis method |
| EP3141885A1 (en) * | 2015-09-14 | 2017-03-15 | Sysmex Corporation | Blood analyzer and blood analyzing method |
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Application publication date: 20200925 |