CN105699380A - Analysis equipment and method for simultaneously measuring CRP (C-Reactive Protein) and blood routine - Google Patents
Analysis equipment and method for simultaneously measuring CRP (C-Reactive Protein) and blood routine Download PDFInfo
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- 238000012360 testing method Methods 0.000 abstract description 31
- 230000036647 reaction Effects 0.000 abstract 3
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- 238000009666 routine test Methods 0.000 abstract 1
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Abstract
The invention discloses analysis equipment and method for simultaneously measuring CRP (C-Reactive Protein) and blood routine. The method comprises the following steps: sucking a sample through a sampling needle; evacuating a CRP measuring tank; then adding a first hemolytic agent into the CRP measuring tank; moving the sampling needle to the CRP measuring tank and adding one part of the blood sample into the CRP measuring tank; evacuating a white blood cell reaction tank, and adding diluting liquid into the white blood cell reaction tank; adding one part of the blood sample into the white blood cell reaction tank and sufficiently and uniformly mixing; sucking one part of a mixed sample from the uniformly mixed sample; evacuating a red blood cell counting tank, and adding the diluting liquid into the red blood cell counting tank; adding the mixed sample into the red blood cell counting tank; counting up red blood cells and blood platelets, and outputting a measuring result. With the adoption of the analysis equipment and method, the CRP test and five types of blood routine tests can be performed simultaneously through the same tube of whole blood, so that the operation is very convenient; a system is simple and reliable, low in cost and small in size, and is very suitable for primary hospitals.
Description
Technical field
The present invention relates to a kind of blood analyser, a kind of in particular analytical equipment and method that can simultaneously measure CRP and routine blood test。
Background technology
CRP (C reactive protein) is a part for body nonspecific immunity mechanism, can the classical pathway of activating complement, strengthen the phagocytosis of leukocyte, regulate lymphocyte or monokaryon/huge and bite systemic-function, promote the generation of macrophage tissue factor, atheromatous plaque also can detect that CRP。After inflammation starts a few hours, CRP just quickly raises, and within 48 hours, just reaches peak value, along with pathological changes disappears, organizes and the recovery CRP of function is down to normal level accordingly。Therefore CRP testing result has extremely important clinical value in hospital。
Current CRP detection mainly has two ways: 1, detecting CRP in biochemistry analyzer, be first centrifuged by blood sample, take out serum, serum, reaction reagent are added reaction tank, measures CRP content by transmittance purifying method, the method is suitable for the hospital of large sample amount；2, using specific protein analyser individually to detect CRP, blood sample, reaction reagent are added reaction tank by craft quantitative appliances, measure CRP content, the method complex operation by scattering turbidimetry method, are suitable for outpatient service and emergency department。
For large hospital, owing to being all equipped with biochemistry analyzer, therefore detect in biochemistry analyzer together with other biochemical class parameters at large hospital CRP。For being not equipped with the small hospital of biochemistry analyzer and part Medium Sized Hospitals for emergency treatment requirement, carry out CRP detection typically via specific protein analyser。
At present a lot of hospitals need in conjunction with WBC (leukocyte) with two parameters of CRP to do clinical diagnosis reference, though biochemistry analyzer can survey CRP but can not survey WBC, specific protein analyser can only survey CRP can not survey WBC, therefore there is the demand simultaneously measuring WBC and CRP in same blood sample；Simultaneously for the demand of urgent diagnosis, needing to measure CRP quickly, easily to use to do clinical diagnosis, existing specific protein analyser needs manual application of sample, complex operation, and biochemistry analyzer is generally adapted that great amount of samples is unified to be analyzed together, therefore simple operation, become a kind of new demand of test CRP flexibly。
Therefore, prior art has yet to be improved and developed。
Summary of the invention
It is an object of the invention to provide a kind of analytical equipment and method that can simultaneously measure CRP and routine blood test, it is intended to solve existing technology and can not measure the problem of CRP and routine blood test and the complex operation of conventional sense CRP, the problems such as speed is slow simultaneously。
Technical scheme is as follows:
A kind of analysis method that can simultaneously measure CRP and routine blood test, it comprises the following steps that
Step S1: draw sample by sampling needle；
Step S2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S3: sampling needle moves to CRP measuring cell, adds part blood sample toward CRP measuring cell；
Step S4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S5: be divided into all of part-blood toward leukocytoreaction pond, and fully mix；
Step S6: part mixing sample drawn by sample after mixing；
Step S7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S8: add mixing sample toward red blood cell count(RBC) pond；
Step S9: erythrocyte and platelet are counted, exports measurement result。
Described method, its also has parallel with step S4:
Step A1: continue to add antibody reagent toward CRP measuring cell；
Step A2: carry out CRP measurement, exports measurement result。
Described method, its with step S7, the parallel leukocyte channel counts process of step S8, step S9 be:
Step B1: add the second hemolytic agent toward leukocytoreaction pond；
Step B2: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out leukocyte DIFF measurement；
Step B3: add the 3rd hemolytic agent toward leukocytoreaction pond；
Step B4: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out leukocyte Baso measurement, carry out hemoglobinometry simultaneously, export measurement result。
The another kind of analysis method that can simultaneously measure CRP and routine blood test, it includes below scheme:
Step S ' 1: draw sample by sampling needle；
Step S ' 2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S ' 3: sampling needle moves to CRP measuring cell, adds part blood sample toward CRP measuring cell；
Step S ' 4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S ' 5: be divided into all of part-blood toward leukocytoreaction pond, and fully mix；
Step S ' 6: part mixing sample drawn by sample after mixing；
Step S ' 7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S ' 8: add mixing sample toward red blood cell count(RBC) pond；
Step S ' 9: erythrocyte and platelet are counted, exports result of calculation。
Described method, its also has parallel with step S ' 4:
Step A ' 1: continue to add reagent toward CRP measuring cell；
Step A ' 2: carry out CRP measurement, exports result of calculation。
Described method, it with step S ' 7, step S ' 8, leukocyte channel counts process parallel for step S ' 9 is:
Step B ' 1: add the second hemolytic agent toward leukocytoreaction pond；
Step B ' 2: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out DIFF, Baso measurement simultaneously, meanwhile can carry out hemoglobinometry by hemoglobinometry device, export result of calculation。
The another kind of analysis method that can simultaneously measure CRP and routine blood test, it comprises the following steps:
Step S " 1: draw sample by sampling needle；
Step S " 2: emptying leukocytoreaction pond, diluent is then added toward leukocytoreaction pond；
Step S " 3: it is divided into all of part-blood toward leukocytoreaction pond, and fully mixes；
Step S " 4: after mixing, part mixing sample drawn by sample；
Step S " 5: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S " 6: mixing sample is added toward red blood cell count(RBC) pond；
Step S " 7: erythrocyte and platelet are counted。
Described method, it is with step S " 5, step S " 6, step S " 7 parallel leukocyte channel counts processes are:
Step B " 1: add the 4th hemolytic agent toward leukocytoreaction pond, form mixing sample；
Step B " 2: the mixing sample of part is drawn by sampling needle；
Step B " 3: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out DIFF, Baso measurement simultaneously, meanwhile can carry out hemoglobinometry by hemoglobinometry device。
Described method, it is with step B " 3 parallel processes also have:
Step A " 1: add antibody reagent toward CRP measuring cell,
Step A " 2: then carry out CRP measurement, export result of calculation。
A kind of analytical equipment that can simultaneously measure CRP and routine blood test, comprising: laser measuring device for measuring, sampling needle, CRP measurement apparatus, CRP reaction tank, red blood cell count(RBC) pond, hemoglobinometry device, leukocytoreaction pond, diluent, the first hemolytic agent, antibody reagent, the second hemolytic agent, the 3rd hemolytic agent, waste liquid discharge device and conveyer device；
Described laser measuring device for measuring entrance connects conveyer device, and outlet connects waste liquid discharge device, sends in laser measuring device for measuring by conveyer device by the sample in leukocytoreaction pond, then adopts laser scattering method leukocyte is classified and counts；Described CRP measurement apparatus connects CRP reaction tank, by turbidimetry, CRP is measured；Described hemoglobinometry device connects leukocytoreaction pond, by colorimetry, the concentration of hemoglobin is measured；Described sampling needle is used for taking blood sample, and connects conveyer device, by conveyer device, the blood sample time segment of collection is injected separately into CRP reaction tank, leukocytoreaction pond and red blood cell count(RBC) pond；The place that described CRP reaction tank reacts together with antibody reagent for blood sample, the first hemolytic agent；The place that described leukocytoreaction pond reacts for blood sample and the second hemolytic agent and the 3rd hemolytic agent one；Described red blood cell count(RBC) pond is used for the erythrocyte in blood and platelet cell are counted；
The pipe connecting structure of described equipment is: described first hemolytic agent connects conveyer device and CRP reaction tank respectively by three-way pipeline；Described antibody reagent connects conveyer device and CRP reaction tank respectively by three-way pipeline；Described second hemolytic agent connects conveyer device and leukocytoreaction pond respectively by three-way pipeline；Described 3rd hemolytic agent connects conveyer device and leukocytoreaction pond respectively by three-way pipeline, described diluent connects CRP reaction tank, red blood cell count(RBC) pond and leukocytoreaction pond respectively by pipeline, and the leakage fluid dram pipeline in described CRP reaction tank, red blood cell count(RBC) pond and leukocytoreaction pond connects waste liquid discharge device。
Beneficial effects of the present invention: the present invention is by providing a kind of simple analysis system that can measure CRP and routine blood test simultaneously, and available same whole blood tests CRP (c reactive protein) and five classification routine blood test tests simultaneously, and operation is very easy；Band CRP reagent refrigerating function simultaneously, puts back to medical refrigerator without individually taking out reagent after testing；And system is simple and reliable, cost is low, volume is little, is highly suitable for basic hospital。Without manual operations, full-automation completes whole measurement process。
Accompanying drawing explanation
Fig. 1 is the principle assumption diagram of the test equipment of embodiment one provided by the invention。
Fig. 2 is embodiment one method flow diagram provided by the invention。
Fig. 3 is the measurement modular construction schematic diagram on CRP measuring cell provided by the invention。
Fig. 4 is reagent refrigerating cellular construction schematic diagram provided by the invention。
Fig. 5 is the principle assumption diagram of the test equipment of embodiment two provided by the invention。
Fig. 6 is embodiment two method flow diagram provided by the invention。
Fig. 7 is the principle assumption diagram of the test equipment of embodiment three provided by the invention。
Fig. 8 is embodiment three method flow diagram provided by the invention。
Fig. 9 is Comparative result schematic diagram provided by the invention。
Detailed description of the invention
Hospitals at Present needs to do clinical judgment in conjunction with WBC and CRP, and current major part instrument can only be surveyed the blood routine parameters such as WBC and maybe can only survey CRP, when especially CRP surveyed by specific protein analyser, needs manual operations, complex operation。The present invention provides a kind of available same whole blood, measures the test Apparatus and method for of the blood routine parameters such as WBC and CRP, it is not necessary to manual operations, full-automation completes whole measurement process simultaneously。
For making the purpose of the present invention, technical scheme and advantage clearly, clearly, developing simultaneously referring to accompanying drawing, the present invention is described in more detail for embodiment。
Embodiment one
As it is shown in figure 1, the present invention provides a kind of analytical equipment that can measure CRP and routine blood test to include simultaneously: laser measuring device for measuring 1, sampling needle 2, CRP measurement apparatus 3, CRP reaction tank 4, red blood cell count(RBC) pond 5, hemoglobinometry device 6, leukocytoreaction pond 7, diluent the 8, first hemolytic agent 9, antibody reagent the 10, second hemolytic agent the 11, the 3rd hemolytic agent 12, waste liquid discharge device 13 and conveyer device 14。
Wherein, described diluent 8 is diluted sample and the reagent cleaned；First hemolytic agent 9 is for CRP Measurement channel lysed erythrocyte and provides the CRP buffer of reaction；Antibody reagent 10 is the reagent participating in CRP antigen antibody reaction；Second hemolytic agent 11 is DIFF (leukocyte differential count passage) hemolytic agent, and function is dissolve the erythrocyte of DIFF classification passage, four class leukocyte is carried out specific process and realizes differentiation；3rd hemolytic agent 12 is Baso/HGB (basophilic granulocyte/hemoglobin) hemolytic agent, is used for dissolving Baso classification passage erythrocyte, measures HGB simultaneously。
Described laser measuring device for measuring 1 entrance connects conveyer device 14, and outlet connects waste liquid discharge device 13, sends in laser measuring device for measuring 1 by conveyer device 14 by the sample in leukocytoreaction pond 7, then adopts laser scattering method leukocyte is classified and counts；
Described CRP measurement apparatus 3 connects CRP reaction tank 4, by turbidimetry, CRP is measured；
Described hemoglobinometry device 6 connects leukocytoreaction pond 7, by colorimetry, the concentration of hemoglobin is measured；
Described sampling needle 2 is used for taking blood sample, and connects conveyer device 14, by conveyer device 14, the blood sample time segment of collection is injected separately into CRP reaction tank 4, leukocytoreaction pond 7 and red blood cell count(RBC) pond 5；
The place that described CRP reaction tank 4 reacts together with antibody reagent 10 for blood sample, the first hemolytic agent 9；
The place that described leukocytoreaction pond 7 reacts for blood sample and the second hemolytic agent 11 and the 3rd hemolytic agent 12 1；
Described red blood cell count(RBC) pond 5 is used for the erythrocyte in blood and platelet cell are counted。
The equipment of survey simultaneously five classification blood routine parameters provided by the invention and CRP is without independent source of the gas and gas circuit unit, whole equipment is provided with a conveyer device 14, whole simple and compact device, good reliability, cost are low, volume is little, very convenient are popularized for basic hospital。The pipe connecting structure of described equipment is: described first hemolytic agent 9 connects conveyer device 14 and CRP reaction tank 4 respectively by three-way pipeline；Described antibody reagent 10 connects conveyer device 14 and CRP reaction tank 4 respectively by three-way pipeline；Described second hemolytic agent 11 connects conveyer device 14 and leukocytoreaction pond 7 respectively by three-way pipeline；Described 3rd hemolytic agent 12 connects conveyer device 14 and leukocytoreaction pond 7 respectively by three-way pipeline。Sent into measuring liquid used in the reaction of correspondence, counting or measurement apparatus by described conveyer device 14；Described diluent 8 connects CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 respectively by pipeline。The leakage fluid dram pipeline in described CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 connects waste liquid discharge device 13。
As in figure 2 it is shown, the method for testing step of the analytical equipment measuring CRP and routine blood test provided by the invention is as follows simultaneously:
Step S1: draw sample by sampling needle；
Step S2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S3: sampling needle moves to CRP measuring cell, adds part blood sample toward CRP measuring cell；
Step S4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S5: be divided into all of part-blood toward leukocytoreaction pond, and fully mix；
Step S6: part mixing sample drawn by sample after mixing；
Step S7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S8: add mixing sample 18 toward red blood cell count(RBC) pond 5；
Step S9: erythrocyte and platelet are counted, exports measurement result。
Parallel with step S4 also has:
Step A1: continue to add antibody reagent toward CRP measuring cell；
Step A2: carry out CRP measurement, exports measurement result。
The leukocyte channel counts process parallel with step S7, step S8, step S9 is:
Step B1: add the second hemolytic agent 11 toward leukocytoreaction pond 7；
Step B2: by conveyer device 14, sample after reaction is sent into laser measuring device for measuring 1 and carry out leukocyte DIFF measurement；
Step B3: add the 3rd hemolytic agent 12 toward leukocytoreaction pond 7；
Step B4: by conveyer device 14, sample after reaction is sent into laser measuring device for measuring 1 and carry out leukocyte Baso measurement, carry out hemoglobinometry simultaneously, export measurement result。
Finally carry out each passage to be carried out。
Shown in Figure 3, CRP measuring cell 4 provided by the invention is provided with measurement assembly: light source 19 and photelectric receiver 20。
Referring to Fig. 4, the present invention also provides for the antibody reagent 10 that CRP tests by reagent refrigerating unit and carries out cold preservation, is made up of fan 20, cooling piece 21, conduction of heat matrix 22 and upper cover 23。After CRP has tested, it is not necessary to independent taking-up antibody reagent 10 is put refrigerator and carried out cold preservation, easy and simple to handle。This reagent refrigerating unit is expansible puts into the many bottles of reagent needing cold preservation。During specific protein analyser test CRP, first need to take out CRP reagent from refrigerator, test again after recovering room temperature。Need again to put back to CRP reagent refrigerator after testing to carry out cold preservation and prevent reagent from going bad。The conduction of heat matrix 22 of antibody reagent 10 preheating tested with refrigerating unit and CRP in test CRP equipment provided by the invention, puts back to refrigerator without manually taking out CRP reagent after testing, and without wait, can carry out CRP test at any time。
Conventional biochemistry analyzer measure CRP can only test sera sample, if to do routine blood test and two projects of CRP, it is necessary to take out secondary blood, bring misery to patient。Also need first blood sample to be centrifuged out sera components when testing CRP by biochemistry analyzer to detect, bring the triviality of operation to operator。Additionally biochemistry analyzer can not detect with peripheral blood, is not suitable for the CRP detection adopting the patient of venous blood difficulty。Method provided by the invention only need to take out a blood, can complete routine blood test and CRP test result in same whole blood sample, convenient and swift。
When using whole blood sample to detect on specific protein analyser, owing to the cells such as erythrocyte can affect the testing result of CRP, therefore the CRP result of whole blood sample has certain deviation。Method provided by the invention can measure hemocyte simultaneously as a result, it is possible to CRP is corrected by the result according to hemocyte, thus ensure that the accuracy of whole blood sample CRP。
In order to add fast response, preheating apparatus can be added in the middle of reagent bottle to the pipeline of reaction tank, reagent is heated rapidly to reaction temperature, course of reaction can be accelerated, shorten gate time。According to specificity of reagent, one or more reagent can be preheated simultaneously, during multichannel reagent, the preheating apparatus of optional each road reagent independence, it is possible to select to share a set of preheating apparatus。
Embodiment two
Referring to Fig. 5, embodiment two and the distinctive points of embodiment one are in that to carry out leukocyte DIFF measurement, leukocyte Baso measures and hemoglobinometry shares a kind of hemolytic agent, save the 3rd hemolytic agent 12 in embodiment one, only the second hemolytic agent 11 need to be individually added into leukocytoreaction pond 7。
The analytical equipment that the present embodiment two provides includes: laser measuring device for measuring 1, sampling needle 2, CRP measurement apparatus 3, CRP reaction tank 4, red blood cell count(RBC) pond 5, hemoglobinometry device 6, leukocytoreaction pond 7, diluent the 8, first hemolytic agent 9, antibody reagent the 10, second hemolytic agent 11, waste liquid discharge device 13 and conveyer device 14。
Described laser measuring device for measuring 1 entrance connects conveyer device 14, and outlet connects waste liquid discharge device 13, sends in laser measuring device for measuring 1 by conveyer device 14 by the sample in leukocytoreaction pond 7, then adopts laser scattering method leukocyte is classified and counts；
Described CRP measurement apparatus 3 connects CRP reaction tank 4, by turbidimetry, CRP is measured；
Described hemoglobinometry device 6 connects leukocytoreaction pond 7, by colorimetry, the concentration of hemoglobin is measured；
Described sampling needle 2 is used for taking blood sample, and connects conveyer device 14, by conveyer device 14, the blood sample time segment of collection is injected separately into CRP reaction tank 4, leukocytoreaction pond 7 and red blood cell count(RBC) pond 5；
The place that described CRP reaction tank 4 reacts together with antibody reagent 10 for blood sample, the first hemolytic agent 9；
The place that described leukocytoreaction pond 7 reacts for blood sample and the second hemolytic agent 11 1；
Described red blood cell count(RBC) pond 5 is used for the erythrocyte in blood and platelet cell are counted。
The pipe connecting structure of described equipment is: described first hemolytic agent 9 connects conveyer device 14 and CRP reaction tank 4 respectively by three-way pipeline；Described antibody reagent 10 connects CRP reaction tank 4；Described second hemolytic agent 11 connects conveyer device 14 and leukocytoreaction pond 7 respectively by three-way pipeline。Sent into measuring liquid used in the reaction of correspondence, counting or measurement apparatus by described conveyer device 14；Described diluent 8 connects CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 respectively by pipeline。The leakage fluid dram pipeline in described CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 connects waste liquid discharge device 13。
As shown in Figure 6, the testing process of embodiment two correspondence is:
Step S ' 1: draw sample by sampling needle；
Step S ' 2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S ' 3: sampling needle moves to CRP measuring cell, adds part blood sample 15 toward CRP measuring cell；
Step S ' 4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S ' 5: be divided into part-blood all 16 toward leukocytoreaction pond, and fully mix；
Step S ' 6: part mixing sample drawn by sample after mixing；
Step S ' 7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S ' 8: add mixing sample toward red blood cell count(RBC) pond；
Step S ' 9: erythrocyte and platelet are counted, exports result of calculation。
Parallel with step S ' 4 also has:
Step A ' 1: continue to add antibody reagent toward CRP measuring cell；
Step A ' 2: carry out CRP measurement, exports result of calculation。
With step S ' 7, step S ' 8, leukocyte channel counts process parallel for step S ' 9 it is:
Step B ' 1: add the second hemolytic agent toward leukocytoreaction pond 7；
Step B ' 2: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out DIFF, Baso measurement simultaneously, meanwhile can pass through hemoglobinometry device 6 and carry out hemoglobinometry, export result of calculation。
Finally carry out each passage to be carried out；And measurement result output, terminate counting。
The requirement of a pair second hemolytic agents 11 of scheme is higher, it is necessary to leukocyte five class cell can be carried out difference process thus ensure that can carry out DIFF measurement on a sample measures with Baso simultaneously simultaneously simultaneously, and hemolytic agent 11 also needs to take into account hemoglobinometry function。
Embodiment three
Referring to Fig. 7, the present invention also provides for the distinctive points of another embodiment, the program and embodiment one and is: cancel the first hemolytic agent 9, only adds a kind of antibody reagent 10 toward a CRP measuring cell 4。Second hemolytic agent 11 replaces with the 4th hemolytic agent 13；Need to take into account the haemolysis function of CRP Measurement channel。The function of the 4th hemolytic agent 13 has: 1, lysed erythrocyte, leukocyte various types of cells difference is processed, can carry out DIFF category measurement；2, lysed erythrocyte, processes leukocyte various types of cells difference, can carry out Baso category measurement；3, lysed erythrocyte, can carry out hemoglobinometry；4, lysed erythrocyte, measures ready for CRP。
The analytical equipment that the present embodiment two provides includes: laser measuring device for measuring 1, sampling needle 2, CRP measurement apparatus 3, CRP reaction tank 4, red blood cell count(RBC) pond 5, hemoglobinometry device 6, leukocytoreaction pond 7, diluent 8, antibody reagent the 10, the 4th hemolytic agent 13, waste liquid discharge device 13 and conveyer device 14。
Described laser measuring device for measuring 1 entrance connects conveyer device 14, and outlet connects waste liquid discharge device 13, sends in laser measuring device for measuring 1 by conveyer device 14 by the sample in leukocytoreaction pond 7, then adopts laser scattering method leukocyte is classified and counts；
Described CRP measurement apparatus 3 connects CRP reaction tank 4, by turbidimetry, CRP is measured；
Described hemoglobinometry device 6 connects leukocytoreaction pond 7, by colorimetry, the concentration of hemoglobin is measured；
Described sampling needle 2 is used for taking blood sample, and connects conveyer device 14, by conveyer device 14, the blood sample time segment of collection is injected separately into CRP reaction tank 4, leukocytoreaction pond 7 and red blood cell count(RBC) pond 5；
The place that described CRP reaction tank 4 reacts together with antibody reagent 10 for blood sample；
The place that described leukocytoreaction pond 7 reacts for blood sample and the 4th hemolytic agent 13 1；
Described red blood cell count(RBC) pond 5 is used for the erythrocyte in blood and platelet cell are counted。
The pipe connecting structure of described equipment is: described antibody reagent 10 connects conveyer device 14 and CRP reaction tank 4 respectively by three-way pipeline；Described 4th hemolytic agent 13 connects conveyer device 14 and leukocytoreaction pond 7 respectively by three-way pipeline。Sent into measuring liquid used in the reaction of correspondence, counting or measurement apparatus by described conveyer device 14；Described diluent 8 connects CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 respectively by pipeline。The leakage fluid dram pipeline in described CRP reaction tank 4, red blood cell count(RBC) pond 5 and leukocytoreaction pond 7 connects waste liquid discharge device 13。
As shown in Figure 8, the testing process of embodiment three correspondence is:
Step S " 1: draw sample by sampling needle 2；
Step S " 2: emptying leukocytoreaction pond 7, then add diluent 8 toward leukocytoreaction pond 7；
Step S " 3: it is divided into part-blood all 16 toward leukocytoreaction pond 7, and fully mixes；
Step S " 4: after mixing, part mixing sample 18 drawn by sample；
Step S " 5: emptying red blood cell count(RBC) pond 5, and add diluent toward red blood cell count(RBC) pond 5；
Step S " 6: add mixing sample 18 toward red blood cell count(RBC) pond 5；
Step S " 7: erythrocyte and platelet are counted, exports result of calculation。
With step S " 5, step S " 6, step S " 7 parallel leukocyte channel counts processes are:
Step B " 1: add the 4th hemolytic agent 13 toward leukocytoreaction pond 7, form mixing sample 25；
Step B " 2: the mixing sample 25 of part is drawn by sampling needle 2；
Step B " 3: by conveyer device 14, sample after reaction is sent into laser measuring device for measuring 1 and carry out DIFF, Baso measurement simultaneously, meanwhile can pass through hemoglobinometry device 6 and carry out hemoglobinometry, export result of calculation。
With step B " 3 parallel processes also have:
Step A " 1: add antibody reagent 10 toward CRP measuring cell 4,
Step A " 2: then carry out CRP measurement, export result of calculation。
Finally carry out each passage to be carried out；And measurement result output, terminate counting。
Advantages of the present invention is as follows: the 1) cellanalyzer of CRP and the fives' classification routine blood test of detection simultaneously provided by the invention, each sense channel parallel detection, test speed is fast, and can export CRP and fives' classification routine blood test result simultaneously；2) band CRP reagent refrigerating system, without individually taking out CRP reagent and put into refrigerator cold-storage, facilitating hospital operation librarian use after having detected；3) illustrating that the machine system of scheme is simple, reliable, system bulk is little, is well suited for basic hospital and carries out routine blood test test and CRP test simultaneously；4) CRP Measurement channel is not affected by routine blood test measurement, CRP measures range of linearity width, can reach 300mg/L, compare with the main brand instrument serum sample result individually testing CRP, there is good dependency, correlation coefficient r reaches 0.998 (as it is shown in figure 9, vertical coordinate is the numerical value of the solution of the present invention, abscissa is the numerical value of existing conventional CRP tester)；5) (first serum sample needs carry out high speed centrifugation acquisition for biochemistry analyzer and the applicable serum sample of specific protein analyser, troublesome poeration) test, hemocyte in whole blood sample can produce impact to measuring, and biochemistry analyzer and specific protein analyser do not have blood cell analysis result, therefore can not carry out affecting correction and draw reliable whole blood CRP result。The present invention program directly carries out routine blood test and CRP test with whole blood, without centrifugal sample, simple to operate, the hemocyte result in routine blood test and CRP result can be obtained due to the program simultaneously, therefore according to the hemocyte situation of whole blood sample, CRP can be modified, it is ensured that CRP result is accurately and reliably。This scheme of the invention also compatible serum sample carries out CRP test, flexible operation, strong adaptability simultaneously。
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, it is possible to improved according to the above description or convert, for instance possible replacement scheme is:
1, CRP reaction tank 4 and leukocytoreaction pond 7 being merged into a reaction tank, this integrated reaction tank has taken into account following functions: 1, the reaction of CRP sample and measurement；2, the reaction of leukocyte DIFF sample and measurement；3, the reaction of leukocyte Baso sample and measurement；4, hemoglobinometry。Owing to CRP reaction and DIFF reaction and Baso reaction can influence each other, the program needs timesharing that projects are individually tested, and projects are tested by serial one by one, and test speed is slow。All these improve and convert the protection domain that all should belong to claims of the present invention。
Claims (10)
1. the analysis method that can simultaneously measure CRP and routine blood test, it is characterised in that comprise the following steps that
Step S1: draw sample by sampling needle；
Step S2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S3: sampling needle moves to CRP measuring cell, adds part blood sample toward CRP measuring cell；
Step S4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S5: be divided into all of part-blood toward leukocytoreaction pond, and fully mix；
Step S6: part mixing sample drawn by sample after mixing；
Step S7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S8: add mixing sample toward red blood cell count(RBC) pond；
Step S9: erythrocyte and platelet are counted, exports measurement result。
2. method according to claim 1, it is characterised in that parallel with step S4 also has:
Step A1: continue to add antibody reagent toward CRP measuring cell；
Step A2: carry out CRP measurement, exports measurement result。
3. method according to claim 2, it is characterised in that the leukocyte channel counts process parallel with step S7, step S8, step S9 is:
Step B1: add the second hemolytic agent toward leukocytoreaction pond；
Step B2: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out leukocyte DIFF measurement；
Step B3: add the 3rd hemolytic agent toward leukocytoreaction pond；
Step B4: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out leukocyte Baso measurement, carry out hemoglobinometry simultaneously, export measurement result。
4. the analysis method that can simultaneously measure CRP and routine blood test, it is characterised in that include below scheme:
Step S ' 1: draw sample by sampling needle；
Step S ' 2: emptying CRP measuring cell, then adds the first hemolytic agent toward CRP measuring cell；
Step S ' 3: sampling needle moves to CRP measuring cell, adds part blood sample toward CRP measuring cell；
Step S ' 4: emptying leukocytoreaction pond, then adds diluent toward leukocytoreaction pond；
Step S ' 5: be divided into all of part-blood toward leukocytoreaction pond, and fully mix；
Step S ' 6: part mixing sample drawn by sample after mixing；
Step S ' 7: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S ' 8: add mixing sample toward red blood cell count(RBC) pond；
Step S ' 9: erythrocyte and platelet are counted, exports result of calculation。
5. method according to claim 4, it is characterised in that parallel with step S ' 4 also has:
Step A ' 1: continue to add reagent toward CRP measuring cell；
Step A ' 2: carry out CRP measurement, exports result of calculation。
6. method according to claim 5, it is characterised in that be with step S ' 7, step S ' 8, leukocyte channel counts process parallel for step S ' 9:
Step B ' 1: add the second hemolytic agent toward leukocytoreaction pond；
Step B ' 2: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out DIFF, Baso measurement simultaneously, meanwhile can carry out hemoglobinometry by hemoglobinometry device, export result of calculation。
7. the analysis method that can simultaneously measure CRP and routine blood test, it is characterised in that comprise the following steps:
Step S " 1: draw sample by sampling needle；
Step S " 2: emptying leukocytoreaction pond, diluent is then added toward leukocytoreaction pond；
Step S " 3: it is divided into all of part-blood toward leukocytoreaction pond, and fully mixes；
Step S " 4: after mixing, part mixing sample drawn by sample；
Step S " 5: emptying red blood cell count(RBC) pond, and diluent is added toward red blood cell count(RBC) pond；
Step S " 6: mixing sample is added toward red blood cell count(RBC) pond；
Step S " 7: erythrocyte and platelet are counted。
8. method according to claim 6, it is characterised in that with step S " 5, step S " 6, step S " 7 parallel leukocyte channel counts processes are:
Step B " 1: add the 4th hemolytic agent toward leukocytoreaction pond, form mixing sample；
Step B " 2: the mixing sample of part is drawn by sampling needle；
Step B " 3: by conveyer device, sample after reaction is sent into laser measuring device for measuring and carry out DIFF, Baso measurement simultaneously, meanwhile can carry out hemoglobinometry by hemoglobinometry device。
9. method according to claim 8, it is characterised in that with step B " 3 parallel processes also have:
Step A " 1: add antibody reagent toward CRP measuring cell,
Step A " 2: then carry out CRP measurement, export result of calculation。
10. the analytical equipment that can simultaneously measure CRP and routine blood test, it is characterized in that, including: laser measuring device for measuring, sampling needle, CRP measurement apparatus, CRP reaction tank, red blood cell count(RBC) pond, hemoglobinometry device, leukocytoreaction pond, diluent, the first hemolytic agent, antibody reagent, the second hemolytic agent, the 3rd hemolytic agent, waste liquid discharge device and conveyer device；
Described laser measuring device for measuring entrance connects conveyer device, and outlet connects waste liquid discharge device, sends in laser measuring device for measuring by conveyer device by the sample in leukocytoreaction pond, then adopts laser scattering method leukocyte is classified and counts；Described CRP measurement apparatus connects CRP reaction tank, by turbidimetry, CRP is measured；Described hemoglobinometry device connects leukocytoreaction pond, by colorimetry, the concentration of hemoglobin is measured；Described sampling needle is used for taking blood sample, and connects conveyer device, by conveyer device, the blood sample time segment of collection is injected separately into CRP reaction tank, leukocytoreaction pond and red blood cell count(RBC) pond；The place that described CRP reaction tank reacts together with antibody reagent for blood sample, the first hemolytic agent；The place that described leukocytoreaction pond reacts for blood sample and the second hemolytic agent and the 3rd hemolytic agent one；Described red blood cell count(RBC) pond is used for the erythrocyte in blood and platelet cell are counted；
The pipe connecting structure of described equipment is: described first hemolytic agent connects conveyer device and CRP reaction tank respectively by three-way pipeline；Described antibody reagent connects conveyer device and CRP reaction tank respectively by three-way pipeline；Described second hemolytic agent connects conveyer device and leukocytoreaction pond respectively by three-way pipeline；Described 3rd hemolytic agent connects conveyer device and leukocytoreaction pond respectively by three-way pipeline, described diluent connects CRP reaction tank, red blood cell count(RBC) pond and leukocytoreaction pond respectively by pipeline, and the leakage fluid dram pipeline in described CRP reaction tank, red blood cell count(RBC) pond and leukocytoreaction pond connects waste liquid discharge device。
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470108A (en) * | 2007-12-24 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Reagent and method for classifying leukocyte |
| JP2012127916A (en) * | 2010-12-17 | 2012-07-05 | Horiba Ltd | Blood analyzer and blood analysis method |
| CN103336130A (en) * | 2013-06-21 | 2013-10-02 | 嘉善加斯戴克医疗器械有限公司 | Whole-blood immunoassay device and blood analyzer with same |
| CN104165834A (en) * | 2013-05-17 | 2014-11-26 | 株式会社堀场制作所 | Blood analysis apparatus |
| CN104297497A (en) * | 2013-07-16 | 2015-01-21 | 成都深迈瑞医疗电子技术研究院有限公司 | Blood analytic method, control device and blood cell analyzing instrument |
| CN104698196A (en) * | 2015-03-04 | 2015-06-10 | 深圳开立生物医疗科技股份有限公司 | Counting pond assembly for five differential hematology analyzers and blood sample distribution method |
| CN104713816A (en) * | 2015-02-04 | 2015-06-17 | 深圳市帝迈生物技术有限公司 | Whole blood CRP detection apparatus, method thereof, and blood cell analyzer |
| CN104833813A (en) * | 2015-05-12 | 2015-08-12 | 江苏英诺华医疗技术有限公司 | Analyzer with blood routine and biochemical detection functions |
-
2016
- 2016-03-23 CN CN201610169537.XA patent/CN105699380A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470108A (en) * | 2007-12-24 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Reagent and method for classifying leukocyte |
| JP2012127916A (en) * | 2010-12-17 | 2012-07-05 | Horiba Ltd | Blood analyzer and blood analysis method |
| CN104165834A (en) * | 2013-05-17 | 2014-11-26 | 株式会社堀场制作所 | Blood analysis apparatus |
| CN103336130A (en) * | 2013-06-21 | 2013-10-02 | 嘉善加斯戴克医疗器械有限公司 | Whole-blood immunoassay device and blood analyzer with same |
| CN104297497A (en) * | 2013-07-16 | 2015-01-21 | 成都深迈瑞医疗电子技术研究院有限公司 | Blood analytic method, control device and blood cell analyzing instrument |
| CN104713816A (en) * | 2015-02-04 | 2015-06-17 | 深圳市帝迈生物技术有限公司 | Whole blood CRP detection apparatus, method thereof, and blood cell analyzer |
| CN104698196A (en) * | 2015-03-04 | 2015-06-10 | 深圳开立生物医疗科技股份有限公司 | Counting pond assembly for five differential hematology analyzers and blood sample distribution method |
| CN104833813A (en) * | 2015-05-12 | 2015-08-12 | 江苏英诺华医疗技术有限公司 | Analyzer with blood routine and biochemical detection functions |
Cited By (35)
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| CN106370584A (en) * | 2016-08-17 | 2017-02-01 | 江苏英诺华医疗技术有限公司 | Blood corpuscle detection and biochemical detection instrument and detection method thereof |
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| CN106473757A (en) * | 2016-09-26 | 2017-03-08 | 北京华为应运科技发展有限公司 | Healthy detecting system |
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